pycom15g03760

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0002206
Physical Location & Seq
Forward (+)
2287771 .. 2293291
5521 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3261 bp
ATGAGCAGCAAGGGAGAAAGTTTTACAAATGGTGTTGACATGGACATCGACGATAGTGACTATGATGATGAACACGATTACTCGACTAGCCTTAACGACCTGGGGGAAGGACTTCTGATGAATTTCTGTAAGGAGGCAGCAACGTCATTCTTCGTCGAGTATGGCCTCATTAGTCATCAGATCAACTCATACAATGACTTTATAAAAAATGGCATACAAAGGGTCTTCAGTTCTTTTGGTGAGATTGTGGTTGAGCCTGGCTATGACCCGTCGAAGAAGGGAGATAACGAATGGCGCTATGCTTTAGTGAAGTTTGGGACTGTTACCCTTGGCAGGCCAAGTTTTTGGGGTGGTTCTGACAATGATAAGGAGTATAATATGTTGCCTAGGCATGCTCGACTTCAGAACATGACATACTGCTCTAAGATGAAAGTGAATATCACGGTCGAGGTATATAATCAACGACTTGTCAGCAGCGATAAGTTTAAGACTGGAAAAGAACGATTTCTTGACAAGGAAATTTTAAGTACAGATACCATAGACATTACAATTGGTAGTGTACATGTGATGGTGAAGTCTGACTTATGCTGGATGAAGGACGTTGAGAAAGGTGACTGTGATTTTGATCACGGAGGCTATTTTATAATCAAGGGTGCAGAGAAGACATTTATTGCACAAGAACAGAGCTGTCTGAAGAAACTTCTCATCACAAATAATCAGGGTCTGACAGTAGCATATAGGTCAGAGGTGAAGAGGCATAGGTTAATAATTAGACTAGTTGGAATTTCTAAACTTGAAAACATTGAAGGAGTAGAGAAAGTCCTTACCGTTTACTTCATGTCGACAGAAATCCCTGTGTGGGTATGGTTTTTTGCGCTTGGCGTTTCATCGGATAGAGAAGTTATTGATCTGATTGATTATGGTAGTGAAGATGCCAGCATTTTGAACATACTTTTTGCCTCAATTCGCGATGCCGATAAAGCTACTGAAAAAGTGGATGGAGGCTTCCGTAGGGGGAAGAATGCCCTCAAATATGTGGATGATGTGATAAAGAAAACTGCATTTCCACCTGGGGAAAGCATCGAAGAGTGCATTAGCTTGTATCTTTTTCCCAATCTCAGAGGCCTAAAGCAAAAGGCTCGCTTTCTTGGGTATATGGTGAAGTCCCTGTTACAAGCCTCTGCTGGTCGCAGGAAATGCGACAATAGGGATGACTTTAGGAACAAGAGGTTGGACTTGGCAGGTGAGCTTCTCGAACGAGAGCTAAGGGCGCATATCGGACATGCGAGGCGGCGCATGGCAAAGGCCTTGCAGAGAGACCTTTACGGTGATCGGGTTGTGCGGCCAATTGAACACTACCTAGATGCCTCCATAGTTACTAATGGCATCTCGAGAGCATTCTCAACTGGAGCATGGTGTCATCCCTTCAAGAGAATGGAGAGGATGTCTGGTGTAGTGGCTAATGTTGGGCGAGCGAATCCGCTGCAGACAATGATTGATATGCGGAAGACAAGGCAGCAGGTTCAATATACAGGGAAGGTTGGAGATGCAAGATACCCGCATCCTTCTCACTGGGGTAAAGTTTGCTTTCTCTCAACCCCAGATGGTGAGAATTGTGGGCTTGTAAAAAATTTGGCTACCACTGGATTAGTAAGTACGAATATATTGGAATCTCTACTACCTGAATTGTCTCTCTGTGGAATGGAGAAATTGGTGGATGATACTTCTACCTCACTGCAAGGGAAGCTCAAAGTTTTTCTGAATGGGGACTGGGTTGGAGTTTGTGAAGATTCCCTCTCATTTGTCTCGGAACTCAGGAGGATGCGACGTAGGAAAAAGTTGCCACCTCAGGTGGAAATCAAAAGAGATGAAAAGCAAGGAGAAGTACGAGTATATTCTGATGCTGGAACGATCCTACGCCCTCTCTTGGTAGTTGAGAATTTGAACAAGATAAAAGCATTTAAAGGGAGAAAGTATCCATTCCAGTATTTTCTTGACAAAGGAATAATCGAGCTTATTGGAGCTGAAGAAGAGGAAGACTGTAGTACTGCATGGGGCATTAGATATCTATTCATGGAAGAAAAGGGAAAATCAGCTGTAAAGTACACACACTGTGAGCTGGACATGTCATTCCTGTTGGGTTTAAGCTGTAGCATTATCCCATTTGCGAATCATGACCATGCAAGGAGGGTCCTCTACCAGGCCCAGAAGCACTCGGCGCAGGCTATTGGGTTTTCTACGACAAATCCTAACCTTCGAGTGGATACGCTGTCTCACCAACTGCACTACCCCCAGAGGCCACTTTTTCAGACAATGACGTCTGACTGCCTTGGCAAACCAGGACACCAACTGGGTCAAAACAGAGTTTTGCCAAAGCCCGAATTGCACAATGGTCAGAATGCGATTGTGGCAGTCAATGTTCACTTGGGATTCAACCAAGAAGACAGCATAGTAATGAACCGGGCATCATTGGAACGTGGTATGTTCCGATCTGATCATATCAGAAGTTACAAGGCTGAGGTTGACAACAAGGACTCCCTGGAAAAAAGGCGGAAGCCTGATGATTGTGTCAATTTTGGCAAGATGCAGAGCAAGTTTGGCCGGGTTGACAATCTCGATGAGGACGGCTTTCCATACGTCGGTGCAAACTTGCAGAGTGGTGATATCATCATCGGGAGGTGCAGTGAATCAGGAGCTGATCATAGCATCAAACTGAAGCACACCGAAAGGGGAATGGTTCAGAAGGTTGTGCTTTCTTCTAATGACGACGGAAAAAACTTTGCTGTTGTTTCTATGAGACAGGTTCGCTCACCGTGTCTTGGAGACAAGTTCTCGAGCATGCATGGGCAAAAGGGGGTTCTAGGTTTTCTGGAGTCCCAAGAGAACTTCCCTTTCACAGTACAAGGGATAGTTCCAGATATTGTTATAAATCCCCATGCATTTCCGTCAAGACAAACTCCTGGTCAACTCTTAGAAGCTGCTTTAGGGAAGGGAATCGCCTCTGGTGGTCAGAAGAGATATGCCACCCCATTCTCTACTCTCTCTGTCGATGACATCGCAAAACAACTGCATAGGGCTGGATTTTCAAGATGCGGAAAAGAGAGAGTATACAGTGGTGGAACCGGTCAAATGGTTCGTTCCCTCATATTCATGGGCCCCACCTTTTATCAGCGCCTGATTCACATGTCTGAAGACAAAGTAAAGTTCCGGAACACTGGGCCAGTCCACCCTCTCACCCGTCAGCCGGTGGCAGATCGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000228 GO:0000418 GO:0000419 GO:0000428 GO:0000785 GO:0000790 GO:0000792 GO:0002682 GO:0003674 GO:0003824 GO:0003899 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005720 GO:0005730 GO:0005736 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009605 GO:0009607 GO:0009620 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016020 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019222 GO:0019438 GO:0030422 GO:0030880 GO:0031047 GO:0031050 GO:0031974 GO:0031981 GO:0032259 GO:0032774 GO:0032991 GO:0034062 GO:0034641 GO:0034654 GO:0035194 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043331 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044451 GO:0044452 GO:0044454 GO:0044464 GO:0044728 GO:0046483 GO:0048519 GO:0048583 GO:0050776 GO:0050789 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0055029 GO:0060255 GO:0061695 GO:0065007 GO:0070013 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0097747 GO:0098542 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1901698 GO:1901699 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko01100 ko03020 ko05016 ko05169 map00230 map00240 map01100 map03020 map05016 map05169
Pfam Domains
Protein Families
RNA_pol_Rpb2_1 RNA_pol_Rpb2_2 RNA_pol_Rpb2_3 RNA_pol_Rpb2_4 RNA_pol_Rpb2_5 RNA_pol_Rpb2_6 RNA_pol_Rpb2_7

Protein Analysis

1087

Amino Acids

122.36

Weight (kDa)

8.75

Isoelectric Point (pI)

38.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNA_pol_Rpb2_1 PF04563 55 - 443 3e-30 RNA polymerase beta subunit
RNA_pol_Rpb2_2 PF04561 268 - 412 3.3e-12 RNA polymerase Rpb2, domain 2
RNA_pol_Rpb2_3 PF04565 491 - 548 1.9e-14 RNA polymerase Rpb2, domain 3
RNA_pol_Rpb2_4 PF04566 585 - 646 9.6e-22 RNA polymerase Rpb2, domain 4
RNA_pol_Rpb2_5 PF04567 661 - 708 1.3e-06 RNA polymerase Rpb2, domain 5
RNA_pol_Rpb2_6 PF00562 717 - 1086 9.4e-109 RNA polymerase Rpb2, domain 6
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 203, 644, 2927
AarI CACCTGC 1 cut(s) 1232
AasI GACNNNNNNGTC 1 cut(s) 2317
AatII GACGTC 1 cut(s) 2321
Acc36I ACCTGC 2 cut(s) 1232, 1510
AccI GTMKAC 2 cut(s) 842, 3108
AccII CGCG 1 cut(s) 969
AccIII TCCGGA 1 cut(s) 3207
AciI CCGC 7 cut(s) 1291, 1342, 1481, 1504, 1559, 2551, 3093
AclWI GGATC 1 cut(s) 1906
AcoI YGGCCR 2 cut(s) 1343, 2599
AcsI RAATTY 5 cut(s) 121, 519, 783, 1630, 1939
AcuI CTGAAG 6 cut(s) 211, 386, 713, 2046, 2735, 3210
AcyI GRCGYC 1 cut(s) 2318
AdeI CACNNNGTG 1 cut(s) 2114
AfaI GTAC 7 cut(s) 529, 561, 1657, 1887, 2047, 2105, 2901
AfiI CCNNNNNNNGG 8 cut(s) 333, 859, 1927, 2200, 2260, 2639, 2819, 3244
AflIII ACRYGT 3 cut(s) 562, 2124, 3183
AgeI ACCGGT 1 cut(s) 3122
AgsI TTSAA 9 cut(s) 797, 806, 946, 1352, 1429, 1526, 1945, 2434, 3087
AhlI ACTAGT 1 cut(s) 775
AjnI CCWGG 7 cut(s) 99, 256, 1069, 2199, 2338, 2538, 2959
AjuI GAANNNNNNNTTGG 2 cut(s) 1214, 1246
Alw26I GTCTC 6 cut(s) 1311, 1695, 1810, 2277, 2791, 2817
AlwI GGATC 1 cut(s) 1906
AlwNI CAGNNNCTG 3 cut(s) 724, 2696, 3175
Ama87I CYCGRG 2 cut(s) 1390, 2833
Aor13HI TCCGGA 1 cut(s) 3207
ApaI GGGCCC 1 cut(s) 3158
ApeKI GCWGC 6 cut(s) 6, 137, 474, 1483, 1516, 2978
ApoI RAATTY 5 cut(s) 121, 519, 783, 1630, 1939
ArsI GACNNNNNNTTYG 4 cut(s) 605, 637, 2947, 2979
AsiGI ACCGGT 1 cut(s) 3122
Asp700I GAANNNNTTC 2 cut(s) 111, 504
AspA2I CCTAGG 1 cut(s) 386
AspLEI GCGC 6 cut(s) 297, 877, 1273, 1296, 2221, 3174
AspS9I GGNCC 5 cut(s) 2191, 2203, 3154, 3155, 3218
AsuC2I CCSGG 2 cut(s) 2462, 2603
AvaI CYCGRG 2 cut(s) 1390, 2833
AvaII GGWCC 1 cut(s) 2191
AvrII CCTAGG 1 cut(s) 386
AxyI CCTNAGG 1 cut(s) 1848
BaeGI GKGCMC 1 cut(s) 3158
BanII GRGCYC 1 cut(s) 3158
BbsI GAAGAC 6 cut(s) 217, 668, 1514, 2043, 2448, 3198
BbvCI CCTCAGC 1 cut(s) 2517
BbvI GCAGC 6 cut(s) 18, 149, 486, 1470, 1528, 2965
BccI CCATC 3 cut(s) 562, 992, 1598
BceAI ACGGC 1 cut(s) 2641
BcgI CGANNNNNNTGC 4 cut(s) 1121, 1155, 1465, 1499
BciT130I CCWGG 7 cut(s) 101, 258, 1071, 2201, 2340, 2540, 2961
BciVI GTATCC 2 cut(s) 1986, 2257
BclI TGATCA 3 cut(s) 625, 2494, 2698
BcnI CCSGG 2 cut(s) 2462, 2603
BcoDI GTCTC 6 cut(s) 1311, 1695, 1810, 2277, 2791, 2817
BcuI ACTAGT 1 cut(s) 775
BfaI CTAG 5 cut(s) 87, 387, 776, 1361, 2861
BfmI CTRYAG 3 cut(s) 1484, 2041, 2149
BfoI RGCGCY 2 cut(s) 298, 3175
BfuAI ACCTGC 2 cut(s) 1232, 1510
BfuI GTATCC 2 cut(s) 1986, 2257
BisI GCNGC 8 cut(s) 7, 138, 475, 1292, 1343, 1484, 1517, 2979
BlnI CCTAGG 1 cut(s) 386
BlsI GCNGC 8 cut(s) 8, 139, 476, 1293, 1344, 1485, 1518, 2980
BmcAI AGTACT 1 cut(s) 2047
Bme1390I CCNGG 9 cut(s) 101, 258, 1071, 2201, 2340, 2462, 2540, 2603, 2961
Bme18I GGWCC 1 cut(s) 2191
BmeT110I CYCGRG 2 cut(s) 1390, 2833
BmgT120I GGNCC 5 cut(s) 2191, 2203, 3154, 3155, 3218
BmiI GGNNCC 4 cut(s) 2192, 3121, 3156, 3157
BmrFI CCNGG 9 cut(s) 101, 258, 1071, 2201, 2340, 2462, 2540, 2603, 2961
BmrI ACTGGG 4 cut(s) 1582, 1780, 2360, 3225
BmuI ACTGGG 4 cut(s) 1582, 1780, 2360, 3225
BpiI GAAGAC 6 cut(s) 217, 668, 1514, 2043, 2448, 3198
BpmI CTGGAG 2 cut(s) 1428, 2891
Bpu10I CCTNAGC 2 cut(s) 1265, 2517
BpuMI CCSGG 2 cut(s) 2462, 2603
Bsa29I ATCGAT 1 cut(s) 3256
BsaBI GATNNNNATC 1 cut(s) 624
BsaHI GRCGYC 1 cut(s) 2318
BsaI GGTCTC 1 cut(s) 1311
BsaJI CCNNGG 6 cut(s) 100, 328, 386, 1070, 2329, 2538
BsaWI WCCGGW 2 cut(s) 3122, 3207
BsaXI ACNNNNNCTCC 6 cut(s) 1430, 1460, 2519, 2549, 2668, 2698
Bsc4I CCNNNNNNNGG 8 cut(s) 333, 859, 1927, 2200, 2260, 2639, 2819, 3244
Bse118I RCCGGY 2 cut(s) 3122, 3244
Bse1I ACTGG 9 cut(s) 496, 1411, 1577, 1648, 1775, 1984, 2355, 3220, 3221
Bse21I CCTNAGG 1 cut(s) 1848
Bse8I GATNNNNATC 1 cut(s) 624
BseAI TCCGGA 1 cut(s) 3207
BseBI CCWGG 7 cut(s) 101, 258, 1071, 2201, 2340, 2540, 2961
BseCI ATCGAT 1 cut(s) 3256
BseDI CCNNGG 6 cut(s) 100, 328, 386, 1070, 2329, 2538
BseGI GGATG 9 cut(s) 597, 1003, 1045, 1216, 1420, 1449, 1561, 1723, 1827
BseJI GATNNNNATC 1 cut(s) 624
BseLI CCNNNNNNNGG 8 cut(s) 333, 859, 1927, 2200, 2260, 2639, 2819, 3244
BseMII CTCAG 4 cut(s) 1132, 1828, 1862, 2508
BseNI ACTGG 9 cut(s) 496, 1411, 1577, 1648, 1775, 1984, 2355, 3220, 3221
BseSI GKGCMC 1 cut(s) 3158
BseXI GCAGC 6 cut(s) 18, 149, 486, 1470, 1528, 2965
BsgI GTGCAG 3 cut(s) 675, 2267, 2701
Bsh1236I CGCG 1 cut(s) 969
Bsh1285I CGRYCG 1 cut(s) 447
BshTI ACCGGT 1 cut(s) 3122
BshVI ATCGAT 1 cut(s) 3256
BsiEI CGRYCG 1 cut(s) 447
BsiHKCI CYCGRG 2 cut(s) 1390, 2833
BsiSI CCGG 5 cut(s) 2461, 2602, 3123, 3208, 3245
BslFI GGGAC 4 cut(s) 331, 1150, 1781, 2860
BslI CCNNNNNNNGG 8 cut(s) 333, 859, 1927, 2200, 2260, 2639, 2819, 3244
BsmAI GTCTC 6 cut(s) 1311, 1695, 1810, 2277, 2791, 2817
BsmFI GGGAC 4 cut(s) 331, 1150, 1781, 2860
BsmI GAATGC 3 cut(s) 1027, 1397, 2404
Bso31I GGTCTC 1 cut(s) 1311
BsoBI CYCGRG 2 cut(s) 1390, 2833
Bsp120I GGGCCC 1 cut(s) 3154
Bsp1286I GDGCHC 1 cut(s) 3158
Bsp13I TCCGGA 1 cut(s) 3207
Bsp1407I TGTACA 1 cut(s) 559
Bsp143I GATC 9 cut(s) 180, 625, 907, 1330, 1911, 2489, 2494, 2698, 3253
Bsp68I TCGCGA 1 cut(s) 969
BspACI CCGC 7 cut(s) 1291, 1342, 1481, 1504, 1559, 2551, 3093
BspCNI CTCAG 4 cut(s) 1131, 1827, 1861, 2509
BspDI ATCGAT 1 cut(s) 3256
BspEI TCCGGA 1 cut(s) 3207
BspFNI CGCG 1 cut(s) 969
BspHI TCATGA 1 cut(s) 2173
BspLI GGNNCC 4 cut(s) 2192, 3121, 3156, 3157
BspMAI CTGCAG 1 cut(s) 1488
BspMI ACCTGC 2 cut(s) 1232, 1510
BspPI GGATC 1 cut(s) 1906
BspTNI GGTCTC 1 cut(s) 1311
BsrFI RCCGGY 2 cut(s) 3122, 3244
BsrGI TGTACA 1 cut(s) 559
BsrI ACTGG 9 cut(s) 496, 1411, 1577, 1648, 1775, 1984, 2355, 3220, 3221
BssAI RCCGGY 2 cut(s) 3122, 3244
BssECI CCNNGG 6 cut(s) 100, 328, 386, 1070, 2329, 2538
BssMI GATC 9 cut(s) 180, 625, 907, 1330, 1911, 2489, 2494, 2698, 3253
BssNAI GTATAC 1 cut(s) 3109
BssNI GRCGYC 1 cut(s) 2318
BssT1I CCWWGG 3 cut(s) 328, 386, 2329
Bst1107I GTATAC 1 cut(s) 3109
Bst2UI CCWGG 7 cut(s) 101, 258, 1071, 2201, 2340, 2540, 2961
Bst6I CTCTTC 4 cut(s) 746, 1080, 2025, 3008
BstACI GRCGYC 1 cut(s) 2318
BstAPI GCANNNNNTGC 1 cut(s) 1197
BstAUI TGTACA 1 cut(s) 559
BstC8I GCNNGC 7 cut(s) 335, 393, 937, 1141, 1473, 2223, 2840
BstDEI CTNAG 7 cut(s) 423, 1118, 1265, 1814, 1848, 2517, 2971
BstENI CCTNNNNNAGG 1 cut(s) 2198
BstF5I GGATG 9 cut(s) 597, 1003, 1045, 1216, 1420, 1449, 1561, 1723, 1827
BstFNI CGCG 1 cut(s) 969
BstH2I RGCGCY 2 cut(s) 298, 3175
BstHHI GCGC 6 cut(s) 297, 877, 1273, 1296, 2221, 3174
BstKTI GATC 9 cut(s) 183, 628, 910, 1333, 1914, 2492, 2497, 2701, 3256
BstMAI GTCTC 6 cut(s) 1311, 1695, 1810, 2277, 2791, 2817
BstMBI GATC 9 cut(s) 180, 625, 907, 1330, 1911, 2489, 2494, 2698, 3253
BstMCI CGRYCG 1 cut(s) 447
BstMWI GCNNNNNNNGC 8 cut(s) 980, 1197, 1270, 1744, 2218, 2383, 2408, 2598
BstNI CCWGG 7 cut(s) 101, 258, 1071, 2201, 2340, 2540, 2961
BstNSI RCATGY 6 cut(s) 395, 566, 1286, 2128, 2842, 3187
BstSCI CCNGG 9 cut(s) 99, 256, 1069, 2199, 2338, 2460, 2538, 2601, 2959
BstSFI CTRYAG 3 cut(s) 1484, 2041, 2149
BstSLI GKGCMC 1 cut(s) 3158
BstUI CGCG 1 cut(s) 969
BstV1I GCAGC 6 cut(s) 18, 149, 486, 1470, 1528, 2965
BstV2I GAAGAC 6 cut(s) 217, 668, 1514, 2043, 2448, 3198
BstXI CCANNNNNNTGG 1 cut(s) 345
BstZ17I GTATAC 1 cut(s) 3109
Bsu15I ATCGAT 1 cut(s) 3256
Bsu36I CCTNAGG 1 cut(s) 1848
BsuI GTATCC 2 cut(s) 1986, 2257
BsuTUI ATCGAT 1 cut(s) 3256
BtgZI GCGATG 2 cut(s) 984, 3040
BtsCI GGATG 9 cut(s) 597, 1003, 1045, 1216, 1420, 1449, 1561, 1723, 1827
BtsI GCAGTG 2 cut(s) 1733, 2689
BtsIMutI CAGTG 7 cut(s) 1570, 1641, 1733, 2110, 2689, 3118, 3213
BtuMI TCGCGA 1 cut(s) 969
BveI ACCTGC 2 cut(s) 1232, 1510
Cac8I GCNNGC 7 cut(s) 335, 393, 937, 1141, 1473, 2223, 2840
CaiI CAGNNNCTG 3 cut(s) 724, 2696, 3175
CciI TCATGA 1 cut(s) 2173
CfoI GCGC 6 cut(s) 297, 877, 1273, 1296, 2221, 3174
Cfr10I RCCGGY 2 cut(s) 3122, 3244
Cfr13I GGNCC 5 cut(s) 2191, 2203, 3154, 3155, 3218
ClaI ATCGAT 1 cut(s) 3256
Csp6I GTAC 7 cut(s) 528, 560, 1656, 1886, 2046, 2104, 2900
CspAI ACCGGT 1 cut(s) 3122
CviQI GTAC 7 cut(s) 528, 560, 1656, 1886, 2046, 2104, 2900
DdeI CTNAG 7 cut(s) 423, 1118, 1265, 1814, 1848, 2517, 2971
DpnI GATC 9 cut(s) 182, 627, 909, 1332, 1913, 2491, 2496, 2700, 3255
DpnII GATC 9 cut(s) 180, 625, 907, 1330, 1911, 2489, 2494, 2698, 3253
DraI TTTAAA 1 cut(s) 1963
DraIII CACNNNGTG 1 cut(s) 2114
DrdI GACNNNNNNGTC 1 cut(s) 2317
DseDI GACNNNNNNGTC 1 cut(s) 2317
EaeI YGGCCR 2 cut(s) 1343, 2599
Eam1104I CTCTTC 4 cut(s) 746, 1080, 2025, 3008
EarI CTCTTC 4 cut(s) 746, 1080, 2025, 3008
EciI GGCGGA 1 cut(s) 2566
Eco130I CCWWGG 3 cut(s) 328, 386, 2329
Eco147I AGGCCT 2 cut(s) 1125, 1307
Eco24I GRGCYC 1 cut(s) 3158
Eco31I GGTCTC 1 cut(s) 1311
Eco32I GATATC 2 cut(s) 2066, 2665
Eco47I GGWCC 1 cut(s) 2191
Eco57I CTGAAG 6 cut(s) 211, 386, 713, 2046, 2735, 3210
Eco81I CCTNAGG 1 cut(s) 1848
Eco88I CYCGRG 2 cut(s) 1390, 2833
EcoNI CCTNNNNNAGG 1 cut(s) 2198
EcoO109I RGGNCCY 2 cut(s) 2191, 3155
EcoRII CCWGG 7 cut(s) 99, 256, 1069, 2199, 2338, 2538, 2959
EcoRV GATATC 2 cut(s) 2066, 2665
EcoT14I CCWWGG 3 cut(s) 328, 386, 2329
EcoT22I ATGCAT 2 cut(s) 2844, 2941
EcoT38I GRGCYC 1 cut(s) 3158
ErhI CCWWGG 3 cut(s) 328, 386, 2329
FalI AAGNNNNNCTT 4 cut(s) 566, 598, 1127, 1159
FaqI GGGAC 4 cut(s) 331, 1150, 1781, 2860
FauI CCCGC 1 cut(s) 1566
FbaI TGATCA 3 cut(s) 625, 2494, 2698
FblI GTMKAC 2 cut(s) 842, 3108
Fnu4HI GCNGC 8 cut(s) 7, 138, 475, 1292, 1343, 1484, 1517, 2979
FokI GGATG 9 cut(s) 604, 1010, 1052, 1223, 1407, 1456, 1548, 1730, 1834
FriOI GRGCYC 1 cut(s) 3158
Fsp4HI GCNGC 8 cut(s) 7, 138, 475, 1292, 1343, 1484, 1517, 2979
FspBI CTAG 5 cut(s) 87, 387, 776, 1361, 2861
GlaI GCGC 6 cut(s) 296, 876, 1272, 1295, 2220, 3173
GluI GCNGC 8 cut(s) 7, 138, 475, 1292, 1343, 1484, 1517, 2979
GsuI CTGGAG 2 cut(s) 1428, 2891
HaeII RGCGCY 2 cut(s) 298, 3175
HapII CCGG 5 cut(s) 2461, 2602, 3123, 3208, 3245
HhaI GCGC 6 cut(s) 297, 877, 1273, 1296, 2221, 3174
Hin1I GRCGYC 1 cut(s) 2318
Hin6I GCGC 6 cut(s) 295, 875, 1271, 1294, 2219, 3172
HinP1I GCGC 6 cut(s) 295, 875, 1271, 1294, 2219, 3172
HincII GTYRAC 5 cut(s) 37, 843, 2524, 2608, 2966
HindII GTYRAC 5 cut(s) 37, 843, 2524, 2608, 2966
HpaII CCGG 5 cut(s) 2461, 2602, 3123, 3208, 3245
Hpy99I CGWCG 6 cut(s) 53, 158, 274, 1830, 2642, 2771
HpyCH4IV ACGT 6 cut(s) 143, 600, 1828, 2318, 2476, 2637
HpyF10VI GCNNNNNNNGC 8 cut(s) 980, 1197, 1270, 1744, 2218, 2383, 2408, 2598
HpyF3I CTNAG 7 cut(s) 423, 1118, 1265, 1814, 1848, 2517, 2971
HpySE526I ACGT 6 cut(s) 143, 600, 1828, 2318, 2476, 2637
Hsp92I GRCGYC 1 cut(s) 2318
HspAI GCGC 6 cut(s) 295, 875, 1271, 1294, 2219, 3172
Kpn2I TCCGGA 1 cut(s) 3207
Ksp22I TGATCA 3 cut(s) 625, 2494, 2698
Kzo9I GATC 9 cut(s) 180, 625, 907, 1330, 1911, 2489, 2494, 2698, 3253
LmnI GCTCC 3 cut(s) 1409, 2021, 2693
Lsp1109I GCAGC 6 cut(s) 18, 149, 486, 1470, 1528, 2965
MaeI CTAG 5 cut(s) 87, 387, 776, 1361, 2861
MaeII ACGT 6 cut(s) 143, 600, 1828, 2318, 2476, 2637
MaeIII GTNAC 6 cut(s) 56, 322, 611, 1170, 1375, 2507
MalI GATC 9 cut(s) 182, 627, 909, 1332, 1913, 2491, 2496, 2700, 3255
MboI GATC 9 cut(s) 180, 625, 907, 1330, 1911, 2489, 2494, 2698, 3253
MfeI CAATTG 2 cut(s) 549, 1347
MhlI GDGCHC 1 cut(s) 3158
MlyI GAGTC 2 cut(s) 2528, 2882
MmeI TCCRAC 4 cut(s) 760, 1212, 1522, 1756
Mph1103I ATGCAT 2 cut(s) 2844, 2941
MroI TCCGGA 1 cut(s) 3207
MroXI GAANNNNTTC 2 cut(s) 111, 504
MseI TTAA 6 cut(s) 93, 486, 524, 764, 1962, 2144
MslI CAYNNNNRTG 3 cut(s) 2178, 2453, 3149
MspA1I CMGCKG 2 cut(s) 1483, 2096
MspI CCGG 5 cut(s) 2461, 2602, 3123, 3208, 3245
MspR9I CCNGG 9 cut(s) 101, 258, 1071, 2201, 2340, 2462, 2540, 2603, 2961
MunI CAATTG 2 cut(s) 549, 1347
Mva1269I GAATGC 3 cut(s) 1027, 1397, 2404
MvaI CCWGG 7 cut(s) 101, 258, 1071, 2201, 2340, 2540, 2961
MvnI CGCG 1 cut(s) 969
MwoI GCNNNNNNNGC 8 cut(s) 980, 1197, 1270, 1744, 2218, 2383, 2408, 2598
NciI CCSGG 2 cut(s) 2462, 2603
NdeII GATC 9 cut(s) 180, 625, 907, 1330, 1911, 2489, 2494, 2698, 3253
NlaIV GGNNCC 4 cut(s) 2192, 3121, 3156, 3157
NmeAIII GCCGAG 1 cut(s) 2195
NmuCI GTSAC 2 cut(s) 56, 611
NruI TCGCGA 1 cut(s) 969
NsiI ATGCAT 2 cut(s) 2844, 2941
NspI RCATGY 6 cut(s) 395, 566, 1286, 2128, 2842, 3187
PaeI GCATGC 2 cut(s) 395, 2842
PaeR7I CTCGAG 2 cut(s) 1390, 2833
PagI TCATGA 1 cut(s) 2173
PaqCI CACCTGC 1 cut(s) 1232
PceI AGGCCT 2 cut(s) 1125, 1307
PciI ACATGT 3 cut(s) 562, 2124, 3183
PctI GAATGC 3 cut(s) 1027, 1397, 2404
PdmI GAANNNNTTC 2 cut(s) 111, 504
PfeI GAWTC 8 cut(s) 1477, 1670, 1790, 2170, 2430, 2687, 2994, 3178
PinAI ACCGGT 1 cut(s) 3122
PkrI GCNGC 8 cut(s) 8, 139, 476, 1293, 1344, 1485, 1518, 2980
PleI GAGTC 2 cut(s) 2528, 2881
PpsI GAGTC 2 cut(s) 2528, 2881
PpuMI RGGWCCY 1 cut(s) 2191
PscI ACATGT 3 cut(s) 562, 2124, 3183
PsiI TTATAA 3 cut(s) 203, 644, 2927
Psp5II RGGWCCY 1 cut(s) 2191
Psp6I CCWGG 7 cut(s) 99, 256, 1069, 2199, 2338, 2538, 2959
PspGI CCWGG 7 cut(s) 99, 256, 1069, 2199, 2338, 2538, 2959
PspN4I GGNNCC 4 cut(s) 2192, 3121, 3156, 3157
PspOMI GGGCCC 1 cut(s) 3154
PspPI GGNCC 5 cut(s) 2191, 2203, 3154, 3155, 3218
PspPPI RGGWCCY 1 cut(s) 2191
PsrI GAACNNNNNNTAC 2 cut(s) 398, 430
PstI CTGCAG 1 cut(s) 1488
PstNI CAGNNNCTG 3 cut(s) 724, 2696, 3175
PvuII CAGCTG 1 cut(s) 2096
RruI TCGCGA 1 cut(s) 969
RsaI GTAC 7 cut(s) 529, 561, 1657, 1887, 2047, 2105, 2901
RsaNI GTAC 7 cut(s) 528, 560, 1656, 1886, 2046, 2104, 2900
RseI CAYNNNNRTG 3 cut(s) 2178, 2453, 3149
SalI GTCGAC 1 cut(s) 841
SaqAI TTAA 6 cut(s) 93, 486, 524, 764, 1962, 2144
SatI GCNGC 8 cut(s) 7, 138, 475, 1292, 1343, 1484, 1517, 2979
Sau3AI GATC 9 cut(s) 180, 625, 907, 1330, 1911, 2489, 2494, 2698, 3253
Sau96I GGNCC 5 cut(s) 2191, 2203, 3154, 3155, 3218
ScaI AGTACT 1 cut(s) 2047
SchI GAGTC 2 cut(s) 2528, 2882
ScrFI CCNGG 9 cut(s) 101, 258, 1071, 2201, 2340, 2462, 2540, 2603, 2961
SduI GDGCHC 1 cut(s) 3158
SfcI CTRYAG 3 cut(s) 1484, 2041, 2149
Sfr274I CTCGAG 2 cut(s) 1390, 2833
SinI GGWCC 1 cut(s) 2191
SlaI CTCGAG 2 cut(s) 1390, 2833
SmiMI CAYNNNNRTG 3 cut(s) 2178, 2453, 3149
SmlI CTYRAG 2 cut(s) 1390, 2833
SmoI CTYRAG 2 cut(s) 1390, 2833
SpeI ACTAGT 1 cut(s) 775
SphI GCATGC 2 cut(s) 395, 2842
SseBI AGGCCT 2 cut(s) 1125, 1307
SsiI CCGC 7 cut(s) 1291, 1342, 1481, 1504, 1559, 2551, 3093
SspMI CTAG 5 cut(s) 87, 387, 776, 1361, 2861
StuI AGGCCT 2 cut(s) 1125, 1307
StyD4I CCNGG 9 cut(s) 99, 256, 1069, 2199, 2338, 2460, 2538, 2601, 2959
StyI CCWWGG 3 cut(s) 328, 386, 2329
TaiI ACGT 6 cut(s) 146, 603, 1831, 2321, 2479, 2640
TatI WGTACW 5 cut(s) 527, 559, 2045, 2103, 2899
TauI GCSGC 2 cut(s) 1294, 1345
TfiI GAWTC 8 cut(s) 1477, 1670, 1790, 2170, 2430, 2687, 2994, 3178
Tru1I TTAA 6 cut(s) 93, 486, 524, 764, 1962, 2144
Tru9I TTAA 6 cut(s) 93, 486, 524, 764, 1962, 2144
TscAI CASTG 7 cut(s) 1577, 1648, 1740, 2117, 2689, 3118, 3220
TseFI GTSAC 2 cut(s) 56, 611
TseI GCWGC 6 cut(s) 6, 137, 474, 1483, 1516, 2978
Tsp45I GTSAC 2 cut(s) 56, 611
TspGWI ACGGA 4 cut(s) 645, 998, 2784, 2934
TspRI CASTG 7 cut(s) 1577, 1648, 1740, 2117, 2689, 3118, 3220
VpaK11BI GGWCC 1 cut(s) 2191
XagI CCTNNNNNAGG 1 cut(s) 2198
XapI RAATTY 5 cut(s) 121, 519, 783, 1630, 1939
XceI RCATGY 6 cut(s) 395, 566, 1286, 2128, 2842, 3187
XhoI CTCGAG 2 cut(s) 1390, 2833
XmaJI CCTAGG 1 cut(s) 386
XmiI GTMKAC 2 cut(s) 842, 3108
XmnI GAANNNNTTC 2 cut(s) 111, 504
XspI CTAG 5 cut(s) 87, 387, 776, 1361, 2861
ZraI GACGTC 1 cut(s) 2319
ZrmI AGTACT 1 cut(s) 2047
Zsp2I ATGCAT 2 cut(s) 2844, 2941
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.