pycom15g06400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0003210
Physical Location & Seq
Reverse (-)
3919021 .. 3919859
839 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 543 bp
ATGAATATATCACCATCACAATGCAATAGTGGATGTGAATCTGGATGGACAAGTTACTTGGATCAGTCCTCTTATCTTTCTCAAAGTCAGGTCCAAAAGCCTGGGGGTTTTGCTGATTATGTGGAGACAAAAGAAGAAGAAGAAGAGGAAGAGGATCTTTCAATGGTTTCTGATGCTTCTTCTGGACCTCCACATTACCACGACTTTGACGAAGACTGCGTGTACGAAAACGGATCTTCTTTTTCGGTTTCGTGGGGTTCCAAATTGGGAAAGAAAAGCAAGAAGAAGAGCAAAGGAAATGGCAGCAGAGAGCTCCACCTTGATGACACCGCCAGCTCCCCAGTCCTCAACTATTCCAAGAAGAAGTTGAGTACTAATTTCTCAAAAGATGAATCTTCAATGCAGAATGTATTGGGTTACTCTGAGGGTTTTTCTGCAACACACAAGAAGGGAAAATCTGCATTGCTGCAGCACTTTGGTTTCTTGAAGTCATCTCTAGCCAAAAGCCCAGCTTCACAAAAACCAGGTGAAAGTTGGGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

181

Amino Acids

19.72

Weight (kDa)

5.42

Isoelectric Point (pI)

51.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 330
AclWI GGATC 3 cut(s) 69, 162, 241
AfaI GTAC 2 cut(s) 224, 373
AgsI TTSAA 3 cut(s) 162, 399, 487
AjnI CCWGG 2 cut(s) 100, 523
AluBI AGCT 3 cut(s) 313, 336, 512
AluI AGCT 3 cut(s) 313, 336, 512
Alw21I GWGCWC 1 cut(s) 315
Alw26I GTCTC 1 cut(s) 119
AlwI GGATC 3 cut(s) 69, 162, 241
ApeKI GCWGC 3 cut(s) 303, 466, 469
AspS9I GGNCC 2 cut(s) 91, 185
AsuHPI GGTGA 2 cut(s) 3, 539
AvaII GGWCC 2 cut(s) 91, 185
BanII GRGCYC 1 cut(s) 315
BbsI GAAGAC 1 cut(s) 219
Bbv12I GWGCWC 1 cut(s) 315
BbvI GCAGC 3 cut(s) 315, 453, 481
BccI CCATC 2 cut(s) 22, 39
BciT130I CCWGG 2 cut(s) 102, 525
BcoDI GTCTC 1 cut(s) 119
BfaI CTAG 1 cut(s) 497
BfmI CTRYAG 1 cut(s) 467
BisI GCNGC 3 cut(s) 304, 467, 470
BlsI GCNGC 3 cut(s) 305, 468, 471
BmcAI AGTACT 1 cut(s) 373
Bme1390I CCNGG 2 cut(s) 102, 525
Bme18I GGWCC 2 cut(s) 91, 185
BmgT120I GGNCC 2 cut(s) 91, 185
BmiI GGNNCC 1 cut(s) 259
BmrFI CCNGG 2 cut(s) 102, 525
BmrI ACTGGG 1 cut(s) 335
BmsI GCATC 1 cut(s) 163
BmuI ACTGGG 1 cut(s) 335
BpiI GAAGAC 1 cut(s) 219
BsaBI GATNNNNATC 1 cut(s) 37
BsaJI CCNNGG 1 cut(s) 101
Bse1I ACTGG 1 cut(s) 341
Bse3DI GCAATG 1 cut(s) 461
Bse8I GATNNNNATC 1 cut(s) 37
BseBI CCWGG 2 cut(s) 102, 525
BseDI CCNNGG 1 cut(s) 101
BseGI GGATG 2 cut(s) 38, 50
BseJI GATNNNNATC 1 cut(s) 37
BseMI GCAATG 1 cut(s) 461
BseMII CTCAG 1 cut(s) 414
BseNI ACTGG 1 cut(s) 341
BseXI GCAGC 3 cut(s) 315, 453, 481
BseYI CCCAGC 1 cut(s) 508
BsiHKAI GWGCWC 1 cut(s) 315
BsmAI GTCTC 1 cut(s) 119
Bsp1286I GDGCHC 1 cut(s) 315
Bsp143I GATC 3 cut(s) 61, 154, 233
BspACI CCGC 1 cut(s) 330
BspCNI CTCAG 1 cut(s) 415
BspLI GGNNCC 1 cut(s) 259
BspMAI CTGCAG 1 cut(s) 471
BspPI GGATC 3 cut(s) 69, 162, 241
BspQI GCTCTTC 1 cut(s) 281
BsrDI GCAATG 1 cut(s) 461
BsrI ACTGG 1 cut(s) 341
BssECI CCNNGG 1 cut(s) 101
BssMI GATC 3 cut(s) 61, 154, 233
Bst2UI CCWGG 2 cut(s) 102, 525
Bst6I CTCTTC 3 cut(s) 138, 144, 281
BstC8I GCNNGC 1 cut(s) 334
BstDEI CTNAG 1 cut(s) 423
BstF5I GGATG 2 cut(s) 38, 50
BstKTI GATC 3 cut(s) 64, 157, 236
BstMAI GTCTC 1 cut(s) 119
BstMBI GATC 3 cut(s) 61, 154, 233
BstNI CCWGG 2 cut(s) 102, 525
BstSCI CCNGG 2 cut(s) 100, 523
BstSFI CTRYAG 1 cut(s) 467
BstV1I GCAGC 3 cut(s) 315, 453, 481
BstV2I GAAGAC 1 cut(s) 219
BstX2I RGATCY 2 cut(s) 154, 233
BstXI CCANNNNNNTGG 1 cut(s) 101
BstYI RGATCY 2 cut(s) 154, 233
BtsCI GGATG 2 cut(s) 38, 50
Cac8I GCNNGC 1 cut(s) 334
Cfr13I GGNCC 2 cut(s) 91, 185
CsiI ACCWGGT 1 cut(s) 523
Csp6I GTAC 2 cut(s) 223, 372
CviJI RGCY 6 cut(s) 100, 313, 336, 500, 507, 512
CviKI_1 RGCY 6 cut(s) 100, 313, 336, 500, 507, 512
CviQI GTAC 2 cut(s) 223, 372
DdeI CTNAG 1 cut(s) 423
DpnI GATC 3 cut(s) 63, 156, 235
DpnII GATC 3 cut(s) 61, 154, 233
Eam1104I CTCTTC 3 cut(s) 138, 144, 281
EarI CTCTTC 3 cut(s) 138, 144, 281
Ecl136II GAGCTC 1 cut(s) 313
Eco24I GRGCYC 1 cut(s) 315
Eco47I GGWCC 2 cut(s) 91, 185
Eco53kI GAGCTC 1 cut(s) 313
EcoICRI GAGCTC 1 cut(s) 313
EcoRII CCWGG 2 cut(s) 100, 523
EcoT38I GRGCYC 1 cut(s) 315
FaiI YATR 2 cut(s) 8, 120
FalI AAGNNNNNCTT 4 cut(s) 141, 173, 496, 528
Fnu4HI GCNGC 3 cut(s) 304, 467, 470
FokI GGATG 2 cut(s) 45, 57
FriOI GRGCYC 1 cut(s) 315
Fsp4HI GCNGC 3 cut(s) 304, 467, 470
FspBI CTAG 1 cut(s) 497
GluI GCNGC 3 cut(s) 304, 467, 470
GsaI CCCAGC 1 cut(s) 512
HinfI GANTC 2 cut(s) 38, 392
HphI GGTGA 2 cut(s) 3, 539
Hpy166II GTNNAC 1 cut(s) 223
Hpy188I TCNGA 2 cut(s) 172, 424
Hpy188III TCNNGA 3 cut(s) 42, 183, 484
Hpy8I GTNNAC 1 cut(s) 223
HpyAV CCTTC 1 cut(s) 442
HpyCH4V TGCA 5 cut(s) 24, 403, 437, 461, 469
HpyF3I CTNAG 1 cut(s) 423
Kzo9I GATC 3 cut(s) 61, 154, 233
LguI GCTCTTC 1 cut(s) 281
LmnI GCTCC 2 cut(s) 318, 341
Lsp1109I GCAGC 3 cut(s) 315, 453, 481
LweI GCATC 1 cut(s) 163
MabI ACCWGGT 1 cut(s) 523
MaeI CTAG 1 cut(s) 497
MaeIII GTNAC 2 cut(s) 53, 416
MalI GATC 3 cut(s) 63, 156, 235
MboI GATC 3 cut(s) 61, 154, 233
MflI RGATCY 2 cut(s) 154, 233
MhlI GDGCHC 1 cut(s) 315
MluCI AATT 2 cut(s) 263, 376
MnlI CCTC 6 cut(s) 79, 139, 145, 198, 356, 418
MslI CAYNNNNRTG 2 cut(s) 19, 321
MspR9I CCNGG 2 cut(s) 102, 525
MvaI CCWGG 2 cut(s) 102, 525
NdeII GATC 3 cut(s) 61, 154, 233
NlaIV GGNNCC 1 cut(s) 259
PciSI GCTCTTC 1 cut(s) 281
PcsI WCGNNNNNNNCGW 2 cut(s) 207, 216
PfeI GAWTC 2 cut(s) 38, 392
PkrI GCNGC 3 cut(s) 305, 468, 471
Psp124BI GAGCTC 1 cut(s) 315
Psp6I CCWGG 2 cut(s) 100, 523
PspFI CCCAGC 1 cut(s) 508
PspGI CCWGG 2 cut(s) 100, 523
PspN4I GGNNCC 1 cut(s) 259
PspPI GGNCC 2 cut(s) 91, 185
PstI CTGCAG 1 cut(s) 471
PsuI RGATCY 2 cut(s) 154, 233
RsaI GTAC 2 cut(s) 224, 373
RsaNI GTAC 2 cut(s) 223, 372
RseI CAYNNNNRTG 2 cut(s) 19, 321
SacI GAGCTC 1 cut(s) 315
SapI GCTCTTC 1 cut(s) 281
SatI GCNGC 3 cut(s) 304, 467, 470
Sau3AI GATC 3 cut(s) 61, 154, 233
Sau96I GGNCC 2 cut(s) 91, 185
ScaI AGTACT 1 cut(s) 373
ScrFI CCNGG 2 cut(s) 102, 525
SduI GDGCHC 1 cut(s) 315
SetI ASST 7 cut(s) 93, 190, 315, 321, 338, 514, 529
SexAI ACCWGGT 1 cut(s) 523
SfaNI GCATC 1 cut(s) 163
SfcI CTRYAG 1 cut(s) 467
SinI GGWCC 2 cut(s) 91, 185
SmiMI CAYNNNNRTG 2 cut(s) 19, 321
Sse9I AATT 2 cut(s) 263, 376
SsiI CCGC 1 cut(s) 330
SspMI CTAG 1 cut(s) 497
SstI GAGCTC 1 cut(s) 315
StyD4I CCNGG 2 cut(s) 100, 523
TasI AATT 2 cut(s) 263, 376
TatI WGTACW 1 cut(s) 371
TfiI GAWTC 2 cut(s) 38, 392
TseI GCWGC 3 cut(s) 303, 466, 469
TspDTI ATGAA 2 cut(s) 17, 405
TspGWI ACGGA 1 cut(s) 246
VpaK11BI GGWCC 2 cut(s) 91, 185
XcmI CCANNNNNNNNNTGG 1 cut(s) 531
XspI CTAG 1 cut(s) 497
ZrmI AGTACT 1 cut(s) 373
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.