pycom15g26110

NEMP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0001753
Physical Location & Seq
Reverse (-)
20799195 .. 20803393
4199 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1440 bp
ATGGCTTCTGCAAATTCGGGTCGGATCTTCGCCTGCATTCTCTGGCTTTTTTTCCTCTCGCTCCCGCTTTCATCCGCCGTCGAACCCTCCAATGAGCATTCCTTAGTCATTTCCGAGTCGGATAGGGTACAATTATCTCATGGCGCGCCAGTAAAAAATTCTCCTGGCTCCAAGCCTGGCATTTCAGTGCTGTGTGAAAGAGTTCACATCCGTGGACTGTCAAGGCTTAAAAATATTGGGAAGTTTGCCAACACGGTGAAGGTGAAGGTATCAGGAACAAATTCAAGCACTCGTATACCGACCTTTGAGGTTTGTTTCCACAGAAATACAACTCTTGGGCTAGGGATGTGCCCTCAAAGCCGTTGGCAGAAGGTTCCCAAGGGCTCCTGGTCAAGGTCCATGTCACCTTTTGAGCACCAACTCTTAGATATACGAACATATGGTTCACCCTTAGAGAGCTTGGAGGTGTCCATTGAAGAAGAATTTGTTAAATATCGCGTGACGTTTTTGATATTGGGCATAATCATGATGAGCTTGGCGTCCCTCCTGAGCAATTCATTAGTATTTTATTACGGCAGTGGAATGGCAATTGGGGTGGTCCTTGTAATATTGATTGTACTTTTTCAGGGGATGAAGCTTCTTCCAACTGGTCGGAAGAATTCTCTTGCAATTTTTGTGTACTCATCTGTGGTTGGTCTGGGATCTTTTCTCCTTAGCTACCTACCTGGGTTATTGCGTACAGTACTCATAGAGATTGGAATCAGCGAAGATATGTATAATCCTTTGGCTATATTTCTTCTGGCTTTTCTTTTTCTGGCTGGAGCATGGTTGGGCTTCTGGGCAGTTCACAAACTCATCCTTACAGAAGATGGATCAATCGATATAATGACATCTCAGTTTGTTAATTGGTCCATCAAAATTCTGGGTGCTACTGTGATTTTTCAGAGTTCTGTCGATCACCTACTGGCAACAGGAGCTATAGTATTTGGATTCATCGTCTCTGCTATACTAAAGAAAATCTTTAGATGGAGATTCCTTCGTCGCGTGTGCAAGATTATGTCTTTCCAATTCTGGAGCTTAGTCATGGCCTTCCAGTATCTGCCAACATTTCCTTCGTCGATATCTACCGGGAAATCGCTGAAAACACCCAAAAAGAACCGCCGCAGATTGGAAATTCCTGATTCTCCGCCTTCTCCACCTTCAGATCCATGGTTATTTCCTTCTACCTTCCACACCACACCTGAAAGGAGAAAATACTCGAAAGAAGAGTGGGACGCATTCACCAGAGAGACAACAACAAAAGCCTTGCAGGAACTTGCTTCTTCTCCTGAGTATCACAGATGGTGCTCCTCAAATGTAGAAAGAATCAGTGTCACTCCCCGGAGCACTAGAAAAGTTGCTGATCAACCGCGCCACTGGTGGCTCCTTTGGTTGTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NEMP

Protein Analysis

480

Amino Acids

54.03

Weight (kDa)

9.82

Isoelectric Point (pI)

54.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NEMP PF10225 155 - 448 2.3e-34 NEMP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 295
AccII CGCG 4 cut(s) 146, 498, 1044, 1411
AciI CCGC 6 cut(s) 65, 75, 1159, 1162, 1187, 1409
AclWI GGATC 4 cut(s) 32, 709, 880, 1199
AcsI RAATTY 7 cut(s) 13, 157, 280, 482, 658, 918, 1173
AcuI CTGAAG 1 cut(s) 1185
AcyI GRCGYC 1 cut(s) 539
AfaI GTAC 5 cut(s) 129, 618, 680, 739, 744
AfiI CCNNNNNNNGG 2 cut(s) 393, 1168
AgsI TTSAA 2 cut(s) 285, 476
AjnI CCWGG 4 cut(s) 163, 175, 386, 724
AjuI GAANNNNNNNTTGG 2 cut(s) 1096, 1128
AleI CACNNNNGTG 1 cut(s) 210
AluBI AGCT 6 cut(s) 459, 534, 637, 717, 977, 1077
AluI AGCT 6 cut(s) 459, 534, 637, 717, 977, 1077
Alw21I GWGCWC 3 cut(s) 417, 1349, 1388
Alw26I GTCTC 2 cut(s) 1003, 1283
AlwI GGATC 4 cut(s) 32, 709, 880, 1199
AlwNI CAGNNNCTG 1 cut(s) 1099
AoxI GGCC 1 cut(s) 1086
ApoI RAATTY 7 cut(s) 13, 157, 280, 482, 658, 918, 1173
AscI GGCGCGCC 1 cut(s) 144
Asp700I GAANNNNTTC 2 cut(s) 201, 280
AspLEI GCGC 3 cut(s) 146, 148, 1413
AspS9I GGNCC 3 cut(s) 396, 598, 909
AsuC2I CCSGG 2 cut(s) 1129, 1381
AsuHPI GGTGA 6 cut(s) 268, 274, 396, 438, 950, 1273
AvaII GGWCC 3 cut(s) 396, 598, 909
BaeGI GKGCMC 1 cut(s) 353
BanII GRGCYC 1 cut(s) 386
Bbv12I GWGCWC 3 cut(s) 417, 1349, 1388
BccI CCATC 4 cut(s) 863, 920, 1020, 1335
BceAI ACGGC 3 cut(s) 62, 345, 589
BciT130I CCWGG 4 cut(s) 165, 177, 388, 726
BclI TGATCA 1 cut(s) 1402
BcnI CCSGG 2 cut(s) 1129, 1381
BcoDI GTCTC 2 cut(s) 1003, 1283
BfaI CTAG 2 cut(s) 341, 1389
BfmI CTRYAG 1 cut(s) 978
BisI GCNGC 1 cut(s) 1162
BlsI GCNGC 1 cut(s) 1163
BmcAI AGTACT 1 cut(s) 744
Bme1390I CCNGG 6 cut(s) 165, 177, 388, 726, 1129, 1381
Bme18I GGWCC 3 cut(s) 396, 598, 909
BmgT120I GGNCC 3 cut(s) 396, 598, 909
BmiI GGNNCC 4 cut(s) 169, 375, 385, 1424
BmrFI CCNGG 6 cut(s) 165, 177, 388, 726, 1129, 1381
BpmI CTGGAG 2 cut(s) 840, 1093
Bpu10I CCTNAGC 2 cut(s) 548, 713
BpuMI CCSGG 2 cut(s) 1129, 1381
Bsa29I ATCGAT 1 cut(s) 879
BsaBI GATNNNNATC 1 cut(s) 758
BsaHI GRCGYC 1 cut(s) 539
BsaJI CCNNGG 5 cut(s) 211, 378, 725, 1208, 1379
BsaXI ACNNNNNCTCC 2 cut(s) 966, 996
Bsc4I CCNNNNNNNGG 2 cut(s) 393, 1168
Bse1I ACTGG 5 cut(s) 149, 652, 969, 1093, 1421
Bse8I GATNNNNATC 1 cut(s) 758
BseBI CCWGG 4 cut(s) 165, 177, 388, 726
BseCI ATCGAT 1 cut(s) 879
BseDI CCNNGG 5 cut(s) 211, 378, 725, 1208, 1379
BseGI GGATG 5 cut(s) 71, 207, 351, 636, 855
BseJI GATNNNNATC 1 cut(s) 758
BseLI CCNNNNNNNGG 2 cut(s) 393, 1168
BseMII CTCAG 3 cut(s) 539, 908, 1320
BseNI ACTGG 5 cut(s) 149, 652, 969, 1093, 1421
BsePI GCGCGC 1 cut(s) 144
BseRI GAGGAG 1 cut(s) 1339
BseSI GKGCMC 1 cut(s) 353
Bsh1236I CGCG 4 cut(s) 146, 498, 1044, 1411
BshFI GGCC 1 cut(s) 1088
BshVI ATCGAT 1 cut(s) 879
BsiHKAI GWGCWC 3 cut(s) 417, 1349, 1388
BsiSI CCGG 2 cut(s) 1128, 1381
BslFI GGGAC 2 cut(s) 526, 1286
BslI CCNNNNNNNGG 2 cut(s) 393, 1168
BsmAI GTCTC 2 cut(s) 1003, 1283
BsmBI CGTCTC 1 cut(s) 1003
BsmFI GGGAC 2 cut(s) 526, 1286
BsmI GAATGC 3 cut(s) 36, 97, 1277
BsnI GGCC 1 cut(s) 1088
Bsp1286I GDGCHC 5 cut(s) 353, 386, 417, 1349, 1388
Bsp143I GATC 6 cut(s) 24, 701, 872, 955, 1204, 1402
Bsp19I CCATGG 1 cut(s) 1208
BspACI CCGC 6 cut(s) 65, 75, 1159, 1162, 1187, 1409
BspANI GGCC 1 cut(s) 1088
BspCNI CTCAG 3 cut(s) 540, 907, 1321
BspDI ATCGAT 1 cut(s) 879
BspFNI CGCG 4 cut(s) 146, 498, 1044, 1411
BspHI TCATGA 1 cut(s) 525
BspLI GGNNCC 4 cut(s) 169, 375, 385, 1424
BspPI GGATC 4 cut(s) 32, 709, 880, 1199
BsrI ACTGG 5 cut(s) 149, 652, 969, 1093, 1421
BssECI CCNNGG 5 cut(s) 211, 378, 725, 1208, 1379
BssHII GCGCGC 1 cut(s) 144
BssMI GATC 6 cut(s) 24, 701, 872, 955, 1204, 1402
BssNAI GTATAC 1 cut(s) 296
BssNI GRCGYC 1 cut(s) 539
BssT1I CCWWGG 2 cut(s) 378, 1208
Bst1107I GTATAC 1 cut(s) 296
Bst2UI CCWGG 4 cut(s) 165, 177, 388, 726
Bst4CI ACNGT 4 cut(s) 219, 256, 742, 934
Bst6I CTCTTC 1 cut(s) 1260
BstACI GRCGYC 1 cut(s) 539
BstC8I GCNNGC 2 cut(s) 34, 146
BstDEI CTNAG 8 cut(s) 103, 424, 451, 548, 713, 894, 1078, 1329
BstDSI CCRYGG 2 cut(s) 211, 1208
BstENI CCTNNNNNAGG 1 cut(s) 391
BstF5I GGATG 5 cut(s) 71, 207, 351, 636, 855
BstFNI CGCG 4 cut(s) 146, 498, 1044, 1411
BstHHI GCGC 3 cut(s) 146, 148, 1413
BstKTI GATC 6 cut(s) 27, 704, 875, 958, 1207, 1405
BstMAI GTCTC 2 cut(s) 1003, 1283
BstMBI GATC 6 cut(s) 24, 701, 872, 955, 1204, 1402
BstMWI GCNNNNNNNGC 2 cut(s) 357, 974
BstNI CCWGG 4 cut(s) 165, 177, 388, 726
BstSCI CCNGG 6 cut(s) 163, 175, 386, 724, 1127, 1379
BstSFI CTRYAG 1 cut(s) 978
BstSLI GKGCMC 1 cut(s) 353
BstUI CGCG 4 cut(s) 146, 498, 1044, 1411
BstX2I RGATCY 3 cut(s) 24, 701, 1204
BstYI RGATCY 3 cut(s) 24, 701, 1204
BstZ17I GTATAC 1 cut(s) 296
Bsu15I ATCGAT 1 cut(s) 879
BsuRI GGCC 1 cut(s) 1088
BsuTUI ATCGAT 1 cut(s) 879
BtgI CCRYGG 2 cut(s) 211, 1208
BtsCI GGATG 5 cut(s) 71, 207, 351, 636, 855
BtsI GCAGTG 1 cut(s) 583
BtsIMutI CAGTG 4 cut(s) 192, 583, 1375, 1414
Cac8I GCNNGC 2 cut(s) 34, 146
CaiI CAGNNNCTG 1 cut(s) 1099
CciI TCATGA 1 cut(s) 525
CfoI GCGC 3 cut(s) 146, 148, 1413
Cfr13I GGNCC 3 cut(s) 396, 598, 909
ClaI ATCGAT 1 cut(s) 879
CseI GACGC 2 cut(s) 528, 1283
Csp6I GTAC 5 cut(s) 128, 617, 679, 738, 743
CspCI CAANNNNNGTGG 2 cut(s) 576, 611
CviAII CATG 6 cut(s) 140, 400, 526, 825, 1084, 1209
CviQI GTAC 5 cut(s) 128, 617, 679, 738, 743
DdeI CTNAG 8 cut(s) 103, 424, 451, 548, 713, 894, 1078, 1329
DpnI GATC 6 cut(s) 26, 703, 874, 957, 1206, 1404
DpnII GATC 6 cut(s) 24, 701, 872, 955, 1204, 1402
Eam1104I CTCTTC 1 cut(s) 1260
EarI CTCTTC 1 cut(s) 1260
EciI GGCGGA 2 cut(s) 64, 1176
Eco130I CCWWGG 2 cut(s) 378, 1208
Eco24I GRGCYC 1 cut(s) 386
Eco32I GATATC 1 cut(s) 1122
Eco47I GGWCC 3 cut(s) 396, 598, 909
Eco57I CTGAAG 1 cut(s) 1185
EcoNI CCTNNNNNAGG 1 cut(s) 391
EcoRI GAATTC 1 cut(s) 658
EcoRII CCWGG 4 cut(s) 163, 175, 386, 724
EcoRV GATATC 1 cut(s) 1122
EcoT14I CCWWGG 2 cut(s) 378, 1208
EcoT38I GRGCYC 1 cut(s) 386
ErhI CCWWGG 2 cut(s) 378, 1208
Esp3I CGTCTC 1 cut(s) 1003
FaeI CATG 6 cut(s) 143, 403, 529, 828, 1087, 1212
FalI AAGNNNNNCTT 2 cut(s) 1004, 1036
FaqI GGGAC 2 cut(s) 526, 1286
FatI CATG 6 cut(s) 139, 399, 525, 824, 1083, 1208
FauI CCCGC 1 cut(s) 72
FauNDI CATATG 1 cut(s) 439
FbaI TGATCA 1 cut(s) 1402
FblI GTMKAC 1 cut(s) 295
Fnu4HI GCNGC 1 cut(s) 1162
FokI GGATG 5 cut(s) 58, 194, 358, 643, 842
FriOI GRGCYC 1 cut(s) 386
Fsp4HI GCNGC 1 cut(s) 1162
FspBI CTAG 2 cut(s) 341, 1389
GlaI GCGC 3 cut(s) 145, 147, 1412
GluI GCNGC 1 cut(s) 1162
GsuI CTGGAG 2 cut(s) 840, 1093
HaeIII GGCC 1 cut(s) 1088
HapII CCGG 2 cut(s) 1128, 1381
HgaI GACGC 2 cut(s) 528, 1283
HhaI GCGC 3 cut(s) 146, 148, 1413
Hin1I GRCGYC 1 cut(s) 539
Hin1II CATG 6 cut(s) 143, 403, 529, 828, 1087, 1212
Hin6I GCGC 3 cut(s) 144, 146, 1411
HinP1I GCGC 3 cut(s) 144, 146, 1411
HindIII AAGCTT 1 cut(s) 635
HinfI GANTC 6 cut(s) 116, 759, 990, 1032, 1181, 1365
HpaII CCGG 2 cut(s) 1128, 1381
HphI GGTGA 6 cut(s) 268, 274, 396, 438, 950, 1273
Hpy166II GTNNAC 6 cut(s) 205, 215, 296, 446, 679, 847
Hpy188I TCNGA 6 cut(s) 24, 115, 121, 654, 945, 1204
Hpy188III TCNNGA 6 cut(s) 273, 526, 547, 1072, 1178, 1328
Hpy8I GTNNAC 6 cut(s) 205, 215, 296, 446, 679, 847
Hpy99I CGWCG 3 cut(s) 83, 1044, 1120
HpyCH4III ACNGT 4 cut(s) 219, 256, 742, 934
HpyCH4IV ACGT 1 cut(s) 503
HpyCH4V TGCA 5 cut(s) 11, 36, 668, 1050, 1309
HpyF10VI GCNNNNNNNGC 2 cut(s) 357, 974
HpyF3I CTNAG 8 cut(s) 103, 424, 451, 548, 713, 894, 1078, 1329
HpySE526I ACGT 1 cut(s) 503
Hsp92I GRCGYC 1 cut(s) 539
Hsp92II CATG 6 cut(s) 143, 403, 529, 828, 1087, 1212
HspAI GCGC 3 cut(s) 144, 146, 1411
Ksp22I TGATCA 1 cut(s) 1402
Kzo9I GATC 6 cut(s) 24, 701, 872, 955, 1204, 1402
LmnI GCTCC 9 cut(s) 66, 173, 389, 821, 974, 1074, 1352, 1383, 1428
MaeI CTAG 2 cut(s) 341, 1389
MaeII ACGT 1 cut(s) 503
MaeIII GTNAC 3 cut(s) 402, 499, 1372
MalI GATC 6 cut(s) 26, 703, 874, 957, 1206, 1404
MboI GATC 6 cut(s) 24, 701, 872, 955, 1204, 1402
MfeI CAATTG 1 cut(s) 588
MflI RGATCY 3 cut(s) 24, 701, 1204
MhlI GDGCHC 5 cut(s) 353, 386, 417, 1349, 1388
MlyI GAGTC 1 cut(s) 125
MmeI TCCRAC 3 cut(s) 99, 632, 668
MnlI CCTC 7 cut(s) 65, 97, 301, 363, 457, 554, 1360
MroXI GAANNNNTTC 2 cut(s) 201, 280
MseI TTAA 3 cut(s) 228, 489, 903
MslI CAYNNNNRTG 3 cut(s) 185, 210, 524
MspI CCGG 2 cut(s) 1128, 1381
MspR9I CCNGG 6 cut(s) 165, 177, 388, 726, 1129, 1381
MunI CAATTG 1 cut(s) 588
Mva1269I GAATGC 3 cut(s) 36, 97, 1277
MvaI CCWGG 4 cut(s) 165, 177, 388, 726
MvnI CGCG 4 cut(s) 146, 498, 1044, 1411
MwoI GCNNNNNNNGC 2 cut(s) 357, 974
NciI CCSGG 2 cut(s) 1129, 1381
NcoI CCATGG 1 cut(s) 1208
NdeI CATATG 1 cut(s) 439
NdeII GATC 6 cut(s) 24, 701, 872, 955, 1204, 1402
NlaIII CATG 6 cut(s) 143, 403, 529, 828, 1087, 1212
NlaIV GGNNCC 4 cut(s) 169, 375, 385, 1424
NmuCI GTSAC 3 cut(s) 402, 499, 1372
OliI CACNNNNGTG 1 cut(s) 210
PagI TCATGA 1 cut(s) 525
PalAI GGCGCGCC 1 cut(s) 144
PauI GCGCGC 1 cut(s) 144
PctI GAATGC 3 cut(s) 36, 97, 1277
PdmI GAANNNNTTC 2 cut(s) 201, 280
PfeI GAWTC 5 cut(s) 759, 990, 1032, 1181, 1365
PkrI GCNGC 1 cut(s) 1163
PleI GAGTC 1 cut(s) 124
PpsI GAGTC 1 cut(s) 124
Psp6I CCWGG 4 cut(s) 163, 175, 386, 724
PspGI CCWGG 4 cut(s) 163, 175, 386, 724
PspN4I GGNNCC 4 cut(s) 169, 375, 385, 1424
PspPI GGNCC 3 cut(s) 396, 598, 909
PstNI CAGNNNCTG 1 cut(s) 1099
PsuI RGATCY 3 cut(s) 24, 701, 1204
PteI GCGCGC 1 cut(s) 144
RsaI GTAC 5 cut(s) 129, 618, 680, 739, 744
RsaNI GTAC 5 cut(s) 128, 617, 679, 738, 743
RseI CAYNNNNRTG 3 cut(s) 185, 210, 524
SaqAI TTAA 3 cut(s) 228, 489, 903
SatI GCNGC 1 cut(s) 1162
Sau3AI GATC 6 cut(s) 24, 701, 872, 955, 1204, 1402
Sau96I GGNCC 3 cut(s) 396, 598, 909
ScaI AGTACT 1 cut(s) 744
SchI GAGTC 1 cut(s) 125
ScrFI CCNGG 6 cut(s) 165, 177, 388, 726, 1129, 1381
SduI GDGCHC 5 cut(s) 353, 386, 417, 1349, 1388
SfcI CTRYAG 1 cut(s) 978
SgsI GGCGCGCC 1 cut(s) 144
SinI GGWCC 3 cut(s) 396, 598, 909
SmiMI CAYNNNNRTG 3 cut(s) 185, 210, 524
SsiI CCGC 6 cut(s) 65, 75, 1159, 1162, 1187, 1409
SspI AATATT 2 cut(s) 235, 609
SspMI CTAG 2 cut(s) 341, 1389
StyD4I CCNGG 6 cut(s) 163, 175, 386, 724, 1127, 1379
StyI CCWWGG 2 cut(s) 378, 1208
TaaI ACNGT 4 cut(s) 219, 256, 742, 934
TaiI ACGT 1 cut(s) 506
TaqI TCGA 5 cut(s) 81, 879, 954, 1118, 1259
TatI WGTACW 3 cut(s) 616, 678, 742
TauI GCSGC 1 cut(s) 1164
TfiI GAWTC 5 cut(s) 759, 990, 1032, 1181, 1365
Tru1I TTAA 3 cut(s) 228, 489, 903
Tru9I TTAA 3 cut(s) 228, 489, 903
TscAI CASTG 4 cut(s) 192, 583, 1375, 1421
TseFI GTSAC 3 cut(s) 402, 499, 1372
Tsp45I GTSAC 3 cut(s) 402, 499, 1372
TspDTI ATGAA 4 cut(s) 60, 546, 647, 982
TspGWI ACGGA 1 cut(s) 200
TspRI CASTG 4 cut(s) 192, 583, 1375, 1421
VpaK11BI GGWCC 3 cut(s) 396, 598, 909
XagI CCTNNNNNAGG 1 cut(s) 391
XapI RAATTY 7 cut(s) 13, 157, 280, 482, 658, 918, 1173
XcmI CCANNNNNNNNNTGG 1 cut(s) 919
XmiI GTMKAC 1 cut(s) 295
XmnI GAANNNNTTC 2 cut(s) 201, 280
XspI CTAG 2 cut(s) 341, 1389
ZrmI AGTACT 1 cut(s) 744
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.