pycom16g03350

protein phosphatase 2C 15

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0002720
Physical Location & Seq
Forward (+)
2085855 .. 2086646
792 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 411 bp
ATGGGCATGCAGCTCAATCCAGGAAGGGGGAGGATTATTTCCTCGATCAAGACTGATGGTCAGCGAGTACCCGGGAATTCTTCATCAGCATTCTCCGCATTTGCGATCTTTGATGGGCACAATGGAAATGCAGCAGCAGTATATACAAGGGAGAATTTGTTAAAGCATGTGTTGGGTGCTATTCCCCGTGGGCTAGGAAGGGAGGAGTGGCTTCAAGCTTTACCTCGAGCCTTGGTTGCTGGCTTTGTGAAGACTGATAAGGATTTCCAGAGCAGAGGTGAAACTTCTGGAACAACAGCTACCTTTGTAATAGTTGATGGATGGACCGTGACAGTTGCATCAGTAGGAGACTCCCGTTGTATCTTAGATACTCAGGGTGGTGCTGTTTCCACCTTAACTGTTCGATCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

137

Amino Acids

14.34

Weight (kDa)

9.5

Isoelectric Point (pI)

20.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 26 - 134 8.2e-14 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 96
AcsI RAATTY 2 cut(s) 76, 154
AfaI GTAC 1 cut(s) 69
AfiI CCNNNNNNNGG 1 cut(s) 26
AgsI TTSAA 1 cut(s) 215
AhdI GACNNNNNGTC 1 cut(s) 57
AjnI CCWGG 1 cut(s) 19
AluBI AGCT 3 cut(s) 13, 218, 299
AluI AGCT 3 cut(s) 13, 218, 299
Alw26I GTCTC 1 cut(s) 342
Ama87I CYCGRG 2 cut(s) 71, 225
ApeKI GCWGC 3 cut(s) 10, 131, 134
ApoI RAATTY 2 cut(s) 76, 154
AspS9I GGNCC 1 cut(s) 324
AsuC2I CCSGG 2 cut(s) 72, 73
AsuHPI GGTGA 1 cut(s) 290
AvaI CYCGRG 2 cut(s) 71, 225
AvaII GGWCC 1 cut(s) 324
BaeGI GKGCMC 1 cut(s) 120
BbsI GAAGAC 1 cut(s) 257
BbvI GCAGC 3 cut(s) 22, 143, 146
BccI CCATC 4 cut(s) 50, 107, 311, 315
BciT130I CCWGG 1 cut(s) 21
BcnI CCSGG 2 cut(s) 72, 73
BcoDI GTCTC 1 cut(s) 342
BfaI CTAG 1 cut(s) 194
BisI GCNGC 3 cut(s) 11, 132, 135
BlsI GCNGC 3 cut(s) 12, 133, 136
Bme1390I CCNGG 3 cut(s) 21, 72, 73
Bme18I GGWCC 1 cut(s) 324
BmeRI GACNNNNNGTC 1 cut(s) 57
BmeT110I CYCGRG 2 cut(s) 71, 225
BmgT120I GGNCC 1 cut(s) 324
BmrFI CCNGG 3 cut(s) 21, 72, 73
BmsI GCATC 1 cut(s) 347
BpiI GAAGAC 1 cut(s) 257
BpuMI CCSGG 2 cut(s) 72, 73
BsaJI CCNNGG 3 cut(s) 71, 187, 231
BsaXI ACNNNNNCTCC 2 cut(s) 143, 173
Bsc4I CCNNNNNNNGG 1 cut(s) 26
BseBI CCWGG 1 cut(s) 21
BseDI CCNNGG 3 cut(s) 71, 187, 231
BseGI GGATG 1 cut(s) 326
BseLI CCNNNNNNNGG 1 cut(s) 26
BseMII CTCAG 1 cut(s) 386
BseRI GAGGAG 1 cut(s) 218
BseSI GKGCMC 1 cut(s) 120
BseXI GCAGC 3 cut(s) 22, 143, 146
BsiHKCI CYCGRG 2 cut(s) 71, 225
BsiSI CCGG 1 cut(s) 72
BslI CCNNNNNNNGG 1 cut(s) 26
BsmAI GTCTC 1 cut(s) 342
BsmI GAATGC 1 cut(s) 89
BsoBI CYCGRG 2 cut(s) 71, 225
Bsp1286I GDGCHC 1 cut(s) 120
Bsp143I GATC 3 cut(s) 45, 105, 404
BspACI CCGC 1 cut(s) 96
BspCNI CTCAG 1 cut(s) 385
BssECI CCNNGG 3 cut(s) 71, 187, 231
BssMI GATC 3 cut(s) 45, 105, 404
BssT1I CCWWGG 1 cut(s) 231
Bst2UI CCWGG 1 cut(s) 21
Bst4CI ACNGT 3 cut(s) 328, 334, 400
BstC8I GCNNGC 2 cut(s) 8, 241
BstDEI CTNAG 2 cut(s) 364, 372
BstDSI CCRYGG 1 cut(s) 187
BstF5I GGATG 1 cut(s) 326
BstKTI GATC 3 cut(s) 48, 108, 407
BstMAI GTCTC 1 cut(s) 342
BstMBI GATC 3 cut(s) 45, 105, 404
BstMWI GCNNNNNNNGC 2 cut(s) 95, 236
BstNI CCWGG 1 cut(s) 21
BstNSI RCATGY 2 cut(s) 10, 170
BstSCI CCNGG 3 cut(s) 19, 70, 71
BstSLI GKGCMC 1 cut(s) 120
BstV1I GCAGC 3 cut(s) 22, 143, 146
BstV2I GAAGAC 1 cut(s) 257
BtgI CCRYGG 1 cut(s) 187
BtsCI GGATG 1 cut(s) 326
Cac8I GCNNGC 2 cut(s) 8, 241
Cfr13I GGNCC 1 cut(s) 324
Cfr9I CCCGGG 1 cut(s) 71
Csp6I GTAC 1 cut(s) 68
CviAII CATG 2 cut(s) 7, 167
CviJI RGCY 7 cut(s) 13, 193, 211, 218, 230, 243, 299
CviKI_1 RGCY 7 cut(s) 13, 193, 211, 218, 230, 243, 299
CviQI GTAC 1 cut(s) 68
DdeI CTNAG 2 cut(s) 364, 372
DpnI GATC 3 cut(s) 47, 107, 406
DpnII GATC 3 cut(s) 45, 105, 404
DriI GACNNNNNGTC 1 cut(s) 57
Eam1105I GACNNNNNGTC 1 cut(s) 57
Eco130I CCWWGG 1 cut(s) 231
Eco47I GGWCC 1 cut(s) 324
Eco88I CYCGRG 2 cut(s) 71, 225
EcoRI GAATTC 1 cut(s) 76
EcoRII CCWGG 1 cut(s) 19
EcoT14I CCWWGG 1 cut(s) 231
ErhI CCWWGG 1 cut(s) 231
FaeI CATG 2 cut(s) 10, 170
FaiI YATR 5 cut(s) 8, 142, 144, 168, 409
FatI CATG 2 cut(s) 6, 166
Fnu4HI GCNGC 3 cut(s) 11, 132, 135
FokI GGATG 1 cut(s) 333
Fsp4HI GCNGC 3 cut(s) 11, 132, 135
FspBI CTAG 1 cut(s) 194
GluI GCNGC 3 cut(s) 11, 132, 135
HapII CCGG 1 cut(s) 72
Hin1II CATG 2 cut(s) 10, 170
HindIII AAGCTT 1 cut(s) 216
HinfI GANTC 1 cut(s) 350
HpaII CCGG 1 cut(s) 72
HphI GGTGA 1 cut(s) 290
Hpy188III TCNNGA 3 cut(s) 49, 268, 288
HpyAV CCTTC 2 cut(s) 18, 192
HpyCH4III ACNGT 3 cut(s) 328, 334, 400
HpyCH4V TGCA 3 cut(s) 10, 131, 338
HpyF10VI GCNNNNNNNGC 2 cut(s) 95, 236
HpyF3I CTNAG 2 cut(s) 364, 372
Hsp92II CATG 2 cut(s) 10, 170
Kzo9I GATC 3 cut(s) 45, 105, 404
LpnPI CCDG 7 cut(s) 6, 33, 85, 225, 273, 281, 359
Lsp1109I GCAGC 3 cut(s) 22, 143, 146
LweI GCATC 1 cut(s) 347
MaeI CTAG 1 cut(s) 194
MaeIII GTNAC 1 cut(s) 328
MalI GATC 3 cut(s) 47, 107, 406
MboI GATC 3 cut(s) 45, 105, 404
MboII GAAGA 2 cut(s) 72, 262
MhlI GDGCHC 1 cut(s) 120
MluCI AATT 2 cut(s) 76, 154
MlyI GAGTC 1 cut(s) 344
MnlI CCTC 5 cut(s) 24, 52, 196, 234, 269
MseI TTAA 2 cut(s) 161, 395
MspI CCGG 1 cut(s) 72
MspR9I CCNGG 3 cut(s) 21, 72, 73
Mva1269I GAATGC 1 cut(s) 89
MvaI CCWGG 1 cut(s) 21
MwoI GCNNNNNNNGC 2 cut(s) 95, 236
NciI CCSGG 2 cut(s) 72, 73
NdeII GATC 3 cut(s) 45, 105, 404
NlaIII CATG 2 cut(s) 10, 170
NmuCI GTSAC 1 cut(s) 328
NspI RCATGY 2 cut(s) 10, 170
PaeI GCATGC 1 cut(s) 10
PaeR7I CTCGAG 1 cut(s) 225
PctI GAATGC 1 cut(s) 89
PfoI TCCNGGA 1 cut(s) 19
PkrI GCNGC 3 cut(s) 12, 133, 136
PleI GAGTC 1 cut(s) 344
PpsI GAGTC 1 cut(s) 344
Psp6I CCWGG 1 cut(s) 19
PspGI CCWGG 1 cut(s) 19
PspPI GGNCC 1 cut(s) 324
PspXI VCTCGAGB 1 cut(s) 225
PsrI GAACNNNNNNTAC 2 cut(s) 283, 315
RsaI GTAC 1 cut(s) 69
RsaNI GTAC 1 cut(s) 68
SaqAI TTAA 2 cut(s) 161, 395
SatI GCNGC 3 cut(s) 11, 132, 135
Sau3AI GATC 3 cut(s) 45, 105, 404
Sau96I GGNCC 1 cut(s) 324
SchI GAGTC 1 cut(s) 344
ScrFI CCNGG 3 cut(s) 21, 72, 73
SduI GDGCHC 1 cut(s) 120
SetI ASST 7 cut(s) 15, 220, 226, 280, 301, 305, 395
SfaNI GCATC 1 cut(s) 347
Sfr274I CTCGAG 1 cut(s) 225
SinI GGWCC 1 cut(s) 324
SlaI CTCGAG 1 cut(s) 225
SmaI CCCGGG 1 cut(s) 73
SmlI CTYRAG 1 cut(s) 225
SmoI CTYRAG 1 cut(s) 225
SphI GCATGC 1 cut(s) 10
Sse9I AATT 2 cut(s) 76, 154
SsiI CCGC 1 cut(s) 96
SspMI CTAG 1 cut(s) 194
StyD4I CCNGG 3 cut(s) 19, 70, 71
StyI CCWWGG 1 cut(s) 231
TaaI ACNGT 3 cut(s) 328, 334, 400
TaqI TCGA 3 cut(s) 44, 226, 403
TasI AATT 2 cut(s) 76, 154
Tru1I TTAA 2 cut(s) 161, 395
Tru9I TTAA 2 cut(s) 161, 395
TseFI GTSAC 1 cut(s) 328
TseI GCWGC 3 cut(s) 10, 131, 134
Tsp45I GTSAC 1 cut(s) 328
TspDTI ATGAA 1 cut(s) 72
TspMI CCCGGG 1 cut(s) 71
VpaK11BI GGWCC 1 cut(s) 324
XapI RAATTY 2 cut(s) 76, 154
XceI RCATGY 2 cut(s) 10, 170
XhoI CTCGAG 1 cut(s) 225
XmaI CCCGGG 1 cut(s) 71
XspI CTAG 1 cut(s) 194
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.