pycom16g03360

protein phosphatase 2C 15

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0002720
Physical Location & Seq
Forward (+)
2087001 .. 2088101
1101 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 633 bp
ATGGATGTTGGGGAGTTTATAGTTCCAATACCATATGTGAAACAAGTCAAGCTTTCAAATGCCGGTGGGAGGCTTATTATTGCTTCTGATGGCATCTGGGATGCTATATCTTCTGAAATGGCAGCACAGTCTTGCCGTGGGTTGCCAGCTGAACTTGCTGCCGGACAAGTTGTGAAGGAGGCATTAAGGTCAAGGGGTCTAAAGGATGATACGACCTGCATAGTGGTTGACATAATCCCACCTGATACTACCGAACAGCCTTCAACTCCGCCAAAAAAGATTAACAAGCTTAGGACCCTGTTGTTCAGAAAGAAGTCGCGTGATTCTGCTAATAAACTATCCAAGAAGCTTTCGGCTGTGGGTATTGTGGAAGAGTTATTTGAAGAAGGGTCTGCGATGCTTGCTGAAAGGTTGGGCAGTGATGAGTGCACACCACAATCAACAACATCTGGGCTATTCATGTGTGCTGTATGCCAGGTTGACCTTGCTCCGAGCGAGGGCATATCGGTTCACGCAGGTTCTATCTTCTCAACCAGTTCAAAGCCTTGGCAAGGGCCGTTCCTCTGTTCTGATTGTCGTAACAAGAAGGATGCCATGGAAGGAAAACGCCCTAGCGGAGTCAGAGTAGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

211

Amino Acids

22.48

Weight (kDa)

8.14

Isoelectric Point (pI)

41.29

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 224, 506
AccII CGCG 1 cut(s) 319
AciI CCGC 2 cut(s) 269, 615
AfiI CCNNNNNNNGG 3 cut(s) 69, 497, 551
AgsI TTSAA 4 cut(s) 57, 264, 383, 540
AjnI CCWGG 1 cut(s) 474
AluBI AGCT 4 cut(s) 52, 149, 289, 349
AluI AGCT 4 cut(s) 52, 149, 289, 349
Alw21I GWGCWC 1 cut(s) 431
Alw44I GTGCAC 1 cut(s) 427
AoxI GGCC 1 cut(s) 554
ApaLI GTGCAC 1 cut(s) 427
ApeKI GCWGC 2 cut(s) 122, 158
AspS9I GGNCC 2 cut(s) 294, 554
AvaII GGWCC 1 cut(s) 294
BaeGI GKGCMC 1 cut(s) 431
Bbv12I GWGCWC 1 cut(s) 431
BbvI GCAGC 2 cut(s) 134, 145
BccI CCATC 1 cut(s) 83
BceAI ACGGC 2 cut(s) 120, 541
BciT130I CCWGG 1 cut(s) 476
BfaI CTAG 1 cut(s) 612
BfuAI ACCTGC 2 cut(s) 224, 506
BisI GCNGC 2 cut(s) 123, 159
BlsI GCNGC 2 cut(s) 124, 160
Bme1390I CCNGG 1 cut(s) 476
Bme18I GGWCC 1 cut(s) 294
BmgT120I GGNCC 2 cut(s) 294, 554
BmiI GGNNCC 1 cut(s) 296
BmrFI CCNGG 1 cut(s) 476
BmsI GCATC 4 cut(s) 91, 102, 387, 580
Bpu10I CCTNAGC 1 cut(s) 290
BsaJI CCNNGG 3 cut(s) 136, 545, 594
BsaXI ACNNNNNCTCC 1 cut(s) 609
Bsc4I CCNNNNNNNGG 3 cut(s) 69, 497, 551
Bse118I RCCGGY 1 cut(s) 62
Bse1I ACTGG 1 cut(s) 534
BseBI CCWGG 1 cut(s) 476
BseDI CCNNGG 3 cut(s) 136, 545, 594
BseGI GGATG 4 cut(s) 10, 106, 211, 595
BseLI CCNNNNNNNGG 3 cut(s) 69, 497, 551
BseNI ACTGG 1 cut(s) 534
BseSI GKGCMC 1 cut(s) 431
BseXI GCAGC 2 cut(s) 134, 145
Bsh1236I CGCG 1 cut(s) 319
BshFI GGCC 1 cut(s) 556
BsiHKAI GWGCWC 1 cut(s) 431
BsiSI CCGG 2 cut(s) 63, 162
BslI CCNNNNNNNGG 3 cut(s) 69, 497, 551
BsnI GGCC 1 cut(s) 556
Bsp1286I GDGCHC 1 cut(s) 431
Bsp19I CCATGG 1 cut(s) 594
BspACI CCGC 2 cut(s) 269, 615
BspANI GGCC 1 cut(s) 556
BspFNI CGCG 1 cut(s) 319
BspLI GGNNCC 1 cut(s) 296
BspMI ACCTGC 2 cut(s) 224, 506
BsrFI RCCGGY 1 cut(s) 62
BsrI ACTGG 1 cut(s) 534
BssAI RCCGGY 1 cut(s) 62
BssECI CCNNGG 3 cut(s) 136, 545, 594
BssT1I CCWWGG 2 cut(s) 545, 594
Bst2UI CCWGG 1 cut(s) 476
Bst4CI ACNGT 1 cut(s) 129
Bst6I CTCTTC 1 cut(s) 366
BstC8I GCNNGC 2 cut(s) 147, 402
BstDEI CTNAG 1 cut(s) 290
BstDSI CCRYGG 2 cut(s) 136, 594
BstENI CCTNNNNNAGG 1 cut(s) 549
BstF5I GGATG 4 cut(s) 10, 106, 211, 595
BstFNI CGCG 1 cut(s) 319
BstMWI GCNNNNNNNGC 2 cut(s) 155, 401
BstNI CCWGG 1 cut(s) 476
BstSCI CCNGG 1 cut(s) 474
BstSLI GKGCMC 1 cut(s) 431
BstUI CGCG 1 cut(s) 319
BstV1I GCAGC 2 cut(s) 134, 145
BsuRI GGCC 1 cut(s) 556
BtgI CCRYGG 2 cut(s) 136, 594
BtgZI GCGATG 1 cut(s) 410
BtsCI GGATG 4 cut(s) 10, 106, 211, 595
BtsI GCAGTG 1 cut(s) 424
BtsIMutI CAGTG 1 cut(s) 424
BveI ACCTGC 2 cut(s) 224, 506
Cac8I GCNNGC 2 cut(s) 147, 402
Cfr10I RCCGGY 1 cut(s) 62
Cfr13I GGNCC 2 cut(s) 294, 554
CviAII CATG 2 cut(s) 460, 595
DdeI CTNAG 1 cut(s) 290
Eam1104I CTCTTC 1 cut(s) 366
EarI CTCTTC 1 cut(s) 366
EciI GGCGGA 1 cut(s) 258
Eco130I CCWWGG 2 cut(s) 545, 594
Eco47I GGWCC 1 cut(s) 294
EcoNI CCTNNNNNAGG 1 cut(s) 549
EcoO109I RGGNCCY 1 cut(s) 294
EcoRII CCWGG 1 cut(s) 474
EcoT14I CCWWGG 2 cut(s) 545, 594
ErhI CCWWGG 2 cut(s) 545, 594
FaeI CATG 2 cut(s) 463, 598
FalI AAGNNNNNCTT 2 cut(s) 36, 68
FatI CATG 2 cut(s) 459, 594
FauNDI CATATG 1 cut(s) 34
Fnu4HI GCNGC 2 cut(s) 123, 159
FokI GGATG 4 cut(s) 17, 113, 218, 602
Fsp4HI GCNGC 2 cut(s) 123, 159
FspBI CTAG 1 cut(s) 612
GluI GCNGC 2 cut(s) 123, 159
HaeIII GGCC 1 cut(s) 556
HapII CCGG 2 cut(s) 63, 162
Hin1II CATG 2 cut(s) 463, 598
HincII GTYRAC 2 cut(s) 229, 481
HindII GTYRAC 2 cut(s) 229, 481
HindIII AAGCTT 3 cut(s) 50, 287, 347
HinfI GANTC 2 cut(s) 323, 618
HpaII CCGG 2 cut(s) 63, 162
Hpy166II GTNNAC 4 cut(s) 229, 429, 481, 511
Hpy188I TCNGA 6 cut(s) 88, 115, 308, 492, 571, 623
Hpy8I GTNNAC 4 cut(s) 229, 429, 481, 511
HpyAV CCTTC 5 cut(s) 169, 270, 380, 580, 593
HpyCH4III ACNGT 1 cut(s) 129
HpyCH4V TGCA 2 cut(s) 219, 429
HpyF10VI GCNNNNNNNGC 2 cut(s) 155, 401
HpyF3I CTNAG 1 cut(s) 290
Hsp92II CATG 2 cut(s) 463, 598
LmnI GCTCC 1 cut(s) 493
Lsp1109I GCAGC 2 cut(s) 134, 145
LweI GCATC 4 cut(s) 91, 102, 387, 580
MaeI CTAG 1 cut(s) 612
MaeIII GTNAC 1 cut(s) 578
MboII GAAGA 4 cut(s) 102, 383, 395, 517
MhlI GDGCHC 1 cut(s) 431
MlyI GAGTC 1 cut(s) 627
MnlI CCTC 4 cut(s) 63, 172, 490, 572
MseI TTAA 2 cut(s) 185, 282
MspA1I CMGCKG 1 cut(s) 149
MspI CCGG 2 cut(s) 63, 162
MspR9I CCNGG 1 cut(s) 476
MvaI CCWGG 1 cut(s) 476
MvnI CGCG 1 cut(s) 319
MwoI GCNNNNNNNGC 2 cut(s) 155, 401
NcoI CCATGG 1 cut(s) 594
NdeI CATATG 1 cut(s) 34
NlaIII CATG 2 cut(s) 463, 598
NlaIV GGNNCC 1 cut(s) 296
PfeI GAWTC 1 cut(s) 323
PkrI GCNGC 2 cut(s) 124, 160
PleI GAGTC 1 cut(s) 626
PpsI GAGTC 1 cut(s) 626
PpuMI RGGWCCY 1 cut(s) 294
Psp5II RGGWCCY 1 cut(s) 294
Psp6I CCWGG 1 cut(s) 474
PspGI CCWGG 1 cut(s) 474
PspN4I GGNNCC 1 cut(s) 296
PspPI GGNCC 2 cut(s) 294, 554
PspPPI RGGWCCY 1 cut(s) 294
PvuII CAGCTG 1 cut(s) 149
SaqAI TTAA 2 cut(s) 185, 282
SatI GCNGC 2 cut(s) 123, 159
Sau96I GGNCC 2 cut(s) 294, 554
SchI GAGTC 1 cut(s) 627
ScrFI CCNGG 1 cut(s) 476
SduI GDGCHC 1 cut(s) 431
SfaNI GCATC 4 cut(s) 91, 102, 387, 580
SinI GGWCC 1 cut(s) 294
SsiI CCGC 2 cut(s) 269, 615
SspMI CTAG 1 cut(s) 612
StyD4I CCNGG 1 cut(s) 474
StyI CCWWGG 2 cut(s) 545, 594
TaaI ACNGT 1 cut(s) 129
TfiI GAWTC 1 cut(s) 323
Tru1I TTAA 2 cut(s) 185, 282
Tru9I TTAA 2 cut(s) 185, 282
TscAI CASTG 1 cut(s) 424
TseI GCWGC 2 cut(s) 122, 158
TspDTI ATGAA 1 cut(s) 448
TspRI CASTG 1 cut(s) 424
VneI GTGCAC 1 cut(s) 427
VpaK11BI GGWCC 1 cut(s) 294
XagI CCTNNNNNAGG 1 cut(s) 549
XspI CTAG 1 cut(s) 612
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.