pycom17g00940

B2 protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0002574
Physical Location & Seq
Forward (+)
681822 .. 683458
1637 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 882 bp
ATGGAGAGCTTGAGCAGCTTTTGGCAGTTGGGTGATGAGCTTCGAGGGCAGTCAAAAGTCGCAGAAGATAACAAATGGTTAATGGCCGCTTCAAAATTGGCTGAGCAGACAAGGGTAAAGGGCGAGCGTATGAATAACCTTGATCTTTCAAAGGGCCCAGTTGAACAAAGGGCGAGGGATAAATTTGGATTCCAGGAAGATAACAAGTTTGAAAGCCAATACTTTAACATGCTGAGCTTGGATTCTAAAGTAAATGAGAATATGTGCAAAAGTTCCTTCAGGAATGGTATGTACAACATGAATGCAGTTTACCATAAGAACAATGCAAGCATTGCGGGAAACATGACTGGTAACAAGTATAACAACAAAGAAATCAACAACAGCAACAATAACAACAACGAATCTGCAAATACAGTTGAGAAAAGGTTCAAGACCTTGCCGGCTACTGAGACGCTCCCAAGAAATGAGGTGCTTGGAGGTTACATCTTTGTATGTAACAATGACACAATGCAGGAAGATTTGAAGCGACAACTATTTGGTTTACCTCCAAGGTATAGAGATTCTGTTCGGGCAATAACACCAGGGTTGCCATTGTTTCTCTACAATTATACAACACACCAGCTGCATGGCATTTTCGAGGCAGCGAGCTTTGGTGGTTCAAACATTGATCGAACTGCTTGGGAAGACAAGAAGTGTAAAGGCGAATCTAGGTTTCCTGCTCAGGTAAGGATCCGTGTGAGAAAAATCTGCAAGGCTTTGGAAGAAGATTCCTTCAGGCCTGTCTTGCACCACTATGATGGTCCCAAGTTCCGTCTTGAGCTGTCAGTTCCTGAGACCCTGAATCTATTGGACCTATGCGAACAAGCGGGCTCTGCAGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dev_Cell_Death

Protein Analysis

294

Amino Acids

33.44

Weight (kDa)

8.48

Isoelectric Point (pI)

40.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dev_Cell_Death PF10539 159 - 285 1.2e-39 Development and cell death domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 87, 335, 866
AclWI GGATC 2 cut(s) 724, 737
AcoI YGGCCR 1 cut(s) 84
AcsI RAATTY 1 cut(s) 182
AcuI CTGAAG 2 cut(s) 262, 757
AfaI GTAC 1 cut(s) 293
AgsI TTSAA 7 cut(s) 93, 150, 164, 212, 430, 523, 660
AjnI CCWGG 2 cut(s) 192, 580
AluBI AGCT 7 cut(s) 9, 18, 40, 237, 622, 648, 820
AluI AGCT 7 cut(s) 9, 18, 40, 237, 622, 648, 820
Alw26I GTCTC 2 cut(s) 443, 827
AlwI GGATC 2 cut(s) 724, 737
AlwNI CAGNNNCTG 1 cut(s) 830
AoxI GGCC 3 cut(s) 84, 154, 776
ApaI GGGCCC 1 cut(s) 158
ApeKI GCWGC 4 cut(s) 15, 622, 641, 875
ApoI RAATTY 1 cut(s) 182
Asp700I GAANNNNTTC 1 cut(s) 425
AspS9I GGNCC 4 cut(s) 154, 155, 800, 850
AsuHPI GGTGA 1 cut(s) 44
AvaII GGWCC 2 cut(s) 800, 850
BaeGI GKGCMC 1 cut(s) 158
BamHI GGATCC 1 cut(s) 729
BanII GRGCYC 2 cut(s) 158, 872
BbsI GAAGAC 1 cut(s) 690
BbvI GCAGC 3 cut(s) 27, 609, 653
BccI CCATC 1 cut(s) 791
BciT130I CCWGG 2 cut(s) 194, 582
BcoDI GTCTC 2 cut(s) 443, 827
BfaI CTAG 1 cut(s) 708
BfmI CTRYAG 1 cut(s) 873
BisI GCNGC 5 cut(s) 16, 87, 623, 642, 876
BlpI GCTNAGC 2 cut(s) 102, 233
BlsI GCNGC 5 cut(s) 17, 88, 624, 643, 877
Bme1390I CCNGG 2 cut(s) 194, 582
Bme18I GGWCC 2 cut(s) 800, 850
BmgT120I GGNCC 4 cut(s) 154, 155, 800, 850
BmiI GGNNCC 3 cut(s) 156, 731, 802
BmrFI CCNGG 2 cut(s) 194, 582
BmrI ACTGGG 1 cut(s) 152
BmuI ACTGGG 1 cut(s) 152
BpiI GAAGAC 1 cut(s) 690
Bpu10I CCTNAGC 1 cut(s) 720
Bpu1102I GCTNAGC 2 cut(s) 102, 233
BpuEI CTTGAG 2 cut(s) 31, 836
BsaI GGTCTC 1 cut(s) 827
BsaJI CCNNGG 2 cut(s) 548, 581
Bse118I RCCGGY 1 cut(s) 439
Bse1I ACTGG 2 cut(s) 158, 352
Bse3DI GCAATG 1 cut(s) 330
BseBI CCWGG 2 cut(s) 194, 582
BseDI CCNNGG 2 cut(s) 548, 581
BseMI GCAATG 1 cut(s) 330
BseMII CTCAG 5 cut(s) 93, 224, 438, 734, 822
BseNI ACTGG 2 cut(s) 158, 352
BseSI GKGCMC 1 cut(s) 158
BseXI GCAGC 3 cut(s) 27, 609, 653
BshFI GGCC 3 cut(s) 86, 156, 778
BsiSI CCGG 1 cut(s) 440
BslFI GGGAC 1 cut(s) 786
BsmAI GTCTC 2 cut(s) 443, 827
BsmBI CGTCTC 1 cut(s) 443
BsmFI GGGAC 1 cut(s) 786
BsmI GAATGC 1 cut(s) 307
BsnI GGCC 3 cut(s) 86, 156, 778
Bso31I GGTCTC 1 cut(s) 827
Bsp120I GGGCCC 1 cut(s) 154
Bsp1286I GDGCHC 2 cut(s) 158, 872
Bsp1407I TGTACA 1 cut(s) 291
Bsp143I GATC 3 cut(s) 142, 667, 729
Bsp1720I GCTNAGC 2 cut(s) 102, 233
BspACI CCGC 3 cut(s) 87, 335, 866
BspANI GGCC 3 cut(s) 86, 156, 778
BspCNI CTCAG 5 cut(s) 94, 225, 439, 733, 823
BspLI GGNNCC 3 cut(s) 156, 731, 802
BspMAI CTGCAG 1 cut(s) 877
BspPI GGATC 2 cut(s) 724, 737
BspTNI GGTCTC 1 cut(s) 827
BsrDI GCAATG 1 cut(s) 330
BsrFI RCCGGY 1 cut(s) 439
BsrGI TGTACA 1 cut(s) 291
BsrI ACTGG 2 cut(s) 158, 352
BssAI RCCGGY 1 cut(s) 439
BssECI CCNNGG 2 cut(s) 548, 581
BssMI GATC 3 cut(s) 142, 667, 729
BssT1I CCWWGG 1 cut(s) 548
Bst2UI CCWGG 2 cut(s) 194, 582
Bst4CI ACNGT 1 cut(s) 415
BstAPI GCANNNNNTGC 1 cut(s) 332
BstAUI TGTACA 1 cut(s) 291
BstC8I GCNNGC 5 cut(s) 125, 328, 441, 646, 868
BstDEI CTNAG 5 cut(s) 102, 233, 447, 720, 831
BstKTI GATC 3 cut(s) 145, 670, 732
BstMAI GTCTC 2 cut(s) 443, 827
BstMBI GATC 3 cut(s) 142, 667, 729
BstMWI GCNNNNNNNGC 5 cut(s) 15, 46, 332, 784, 872
BstNI CCWGG 2 cut(s) 194, 582
BstNSI RCATGY 1 cut(s) 232
BstSCI CCNGG 2 cut(s) 192, 580
BstSFI CTRYAG 1 cut(s) 873
BstSLI GKGCMC 1 cut(s) 158
BstV1I GCAGC 3 cut(s) 27, 609, 653
BstV2I GAAGAC 1 cut(s) 690
BstX2I RGATCY 1 cut(s) 729
BstXI CCANNNNNNTGG 2 cut(s) 626, 797
BstYI RGATCY 1 cut(s) 729
BsuRI GGCC 3 cut(s) 86, 156, 778
Cac8I GCNNGC 5 cut(s) 125, 328, 441, 646, 868
CaiI CAGNNNCTG 1 cut(s) 830
Cfr10I RCCGGY 1 cut(s) 439
Cfr13I GGNCC 4 cut(s) 154, 155, 800, 850
CseI GACGC 1 cut(s) 460
Csp6I GTAC 1 cut(s) 292
CviAII CATG 4 cut(s) 229, 298, 343, 626
CviQI GTAC 1 cut(s) 292
DdeI CTNAG 5 cut(s) 102, 233, 447, 720, 831
DpnI GATC 3 cut(s) 144, 669, 731
DpnII GATC 3 cut(s) 142, 667, 729
EaeI YGGCCR 1 cut(s) 84
Eco130I CCWWGG 1 cut(s) 548
Eco147I AGGCCT 1 cut(s) 778
Eco24I GRGCYC 2 cut(s) 158, 872
Eco31I GGTCTC 1 cut(s) 827
Eco47I GGWCC 2 cut(s) 800, 850
Eco57I CTGAAG 2 cut(s) 262, 757
EcoO109I RGGNCCY 1 cut(s) 154
EcoRII CCWGG 2 cut(s) 192, 580
EcoT14I CCWWGG 1 cut(s) 548
EcoT38I GRGCYC 2 cut(s) 158, 872
ErhI CCWWGG 1 cut(s) 548
Esp3I CGTCTC 1 cut(s) 443
FaeI CATG 4 cut(s) 232, 301, 346, 629
FaqI GGGAC 1 cut(s) 786
FatI CATG 4 cut(s) 228, 297, 342, 625
FauI CCCGC 2 cut(s) 328, 859
Fnu4HI GCNGC 5 cut(s) 16, 87, 623, 642, 876
FriOI GRGCYC 2 cut(s) 158, 872
Fsp4HI GCNGC 5 cut(s) 16, 87, 623, 642, 876
FspBI CTAG 1 cut(s) 708
GluI GCNGC 5 cut(s) 16, 87, 623, 642, 876
HaeIII GGCC 3 cut(s) 86, 156, 778
HapII CCGG 1 cut(s) 440
HgaI GACGC 1 cut(s) 460
Hin1II CATG 4 cut(s) 232, 301, 346, 629
HinfI GANTC 7 cut(s) 189, 242, 401, 560, 704, 767, 841
HpaII CCGG 1 cut(s) 440
HphI GGTGA 1 cut(s) 44
Hpy166II GTNNAC 2 cut(s) 310, 542
Hpy188III TCNNGA 4 cut(s) 280, 430, 815, 830
Hpy8I GTNNAC 2 cut(s) 310, 542
HpyAV CCTTC 2 cut(s) 286, 781
HpyCH4III ACNGT 1 cut(s) 415
HpyCH4V TGCA 9 cut(s) 267, 305, 326, 407, 511, 625, 750, 787, 875
HpyF10VI GCNNNNNNNGC 5 cut(s) 15, 46, 332, 784, 872
HpyF3I CTNAG 5 cut(s) 102, 233, 447, 720, 831
Hsp92II CATG 4 cut(s) 232, 301, 346, 629
KroI GCCGGC 1 cut(s) 439
KroNI GCCGGC 1 cut(s) 441
Kzo9I GATC 3 cut(s) 142, 667, 729
LmnI GCTCC 1 cut(s) 459
Lsp1109I GCAGC 3 cut(s) 27, 609, 653
MaeI CTAG 1 cut(s) 708
MaeIII GTNAC 3 cut(s) 350, 479, 494
MalI GATC 3 cut(s) 144, 669, 731
MboI GATC 3 cut(s) 142, 667, 729
MboII GAAGA 6 cut(s) 77, 209, 527, 695, 773, 776
MflI RGATCY 1 cut(s) 729
MhlI GDGCHC 2 cut(s) 158, 872
MluCI AATT 3 cut(s) 95, 182, 604
MnlI CCTC 6 cut(s) 38, 168, 460, 470, 555, 631
MroNI GCCGGC 1 cut(s) 439
MroXI GAANNNNTTC 1 cut(s) 425
MseI TTAA 2 cut(s) 80, 225
MslI CAYNNNNRTG 2 cut(s) 792, 795
MspA1I CMGCKG 1 cut(s) 622
MspI CCGG 1 cut(s) 440
MspR9I CCNGG 2 cut(s) 194, 582
Mva1269I GAATGC 1 cut(s) 307
MvaI CCWGG 2 cut(s) 194, 582
MwoI GCNNNNNNNGC 5 cut(s) 15, 46, 332, 784, 872
NaeI GCCGGC 1 cut(s) 441
NdeII GATC 3 cut(s) 142, 667, 729
NgoMIV GCCGGC 1 cut(s) 439
NlaIII CATG 4 cut(s) 232, 301, 346, 629
NlaIV GGNNCC 3 cut(s) 156, 731, 802
NspI RCATGY 1 cut(s) 232
PceI AGGCCT 1 cut(s) 778
PctI GAATGC 1 cut(s) 307
PdiI GCCGGC 1 cut(s) 441
PdmI GAANNNNTTC 1 cut(s) 425
PfeI GAWTC 7 cut(s) 189, 242, 401, 560, 704, 767, 841
PfoI TCCNGGA 1 cut(s) 192
PkrI GCNGC 5 cut(s) 17, 88, 624, 643, 877
Psp6I CCWGG 2 cut(s) 192, 580
PspGI CCWGG 2 cut(s) 192, 580
PspN4I GGNNCC 3 cut(s) 156, 731, 802
PspOMI GGGCCC 1 cut(s) 154
PspPI GGNCC 4 cut(s) 154, 155, 800, 850
PstI CTGCAG 1 cut(s) 877
PstNI CAGNNNCTG 1 cut(s) 830
PsuI RGATCY 1 cut(s) 729
PvuII CAGCTG 1 cut(s) 622
RsaI GTAC 1 cut(s) 293
RsaNI GTAC 1 cut(s) 292
RseI CAYNNNNRTG 2 cut(s) 792, 795
SaqAI TTAA 2 cut(s) 80, 225
SatI GCNGC 5 cut(s) 16, 87, 623, 642, 876
Sau3AI GATC 3 cut(s) 142, 667, 729
Sau96I GGNCC 4 cut(s) 154, 155, 800, 850
ScrFI CCNGG 2 cut(s) 194, 582
SduI GDGCHC 2 cut(s) 158, 872
SfcI CTRYAG 1 cut(s) 873
SinI GGWCC 2 cut(s) 800, 850
SmiMI CAYNNNNRTG 2 cut(s) 792, 795
SmlI CTYRAG 2 cut(s) 10, 815
SmoI CTYRAG 2 cut(s) 10, 815
Sse9I AATT 3 cut(s) 95, 182, 604
SseBI AGGCCT 1 cut(s) 778
SsiI CCGC 3 cut(s) 87, 335, 866
SspMI CTAG 1 cut(s) 708
StuI AGGCCT 1 cut(s) 778
StyD4I CCNGG 2 cut(s) 192, 580
StyI CCWWGG 1 cut(s) 548
TaaI ACNGT 1 cut(s) 415
TaqI TCGA 3 cut(s) 43, 636, 670
TasI AATT 3 cut(s) 95, 182, 604
TatI WGTACW 1 cut(s) 291
TauI GCSGC 1 cut(s) 89
TfiI GAWTC 7 cut(s) 189, 242, 401, 560, 704, 767, 841
Tru1I TTAA 2 cut(s) 80, 225
Tru9I TTAA 2 cut(s) 80, 225
TseI GCWGC 4 cut(s) 15, 622, 641, 875
TspDTI ATGAA 2 cut(s) 146, 314
TspGWI ACGGA 2 cut(s) 722, 800
VpaK11BI GGWCC 2 cut(s) 800, 850
XapI RAATTY 1 cut(s) 182
XceI RCATGY 1 cut(s) 232
XmnI GAANNNNTTC 1 cut(s) 425
XspI CTAG 1 cut(s) 708
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.