Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr17

Orthogroup ID

OG0001061

Physical Location & Seq

Genomic Coordinates Reverse (-)

6978294 .. 6982357

Gene Structure 4064 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2820 bp

ATGAACTGGAGGCCTAGCCATGTATGCAAGACAAGAACACTGCTGGGATTGGTCTTTTGCATGTGGGTCTCCATGGAAGTGATGGCTGGGACTGAAAATGCCACCCACTCGTCTGCAAGGCCGAGTACTCTGAATATTGGGGCGCTGTTTACTTTCCATTCGGTTATTGGAAGGGCGGCCAAGCCAGCGATTTTAGCTGCAATTGAGGATGTCAATTCTGATCAGAGCGTTCTTCGCGGGACTAAATTGAACGTTATTTTCCATGATACGAATTGCAGCGGATTTCTTGGAACTGTTGAAGCTCTGCAGCTGATTGAAAACGATGTGGTTGCTGCAATTGGCCCGCAATCCTCCGGAATAGCTCATGTCATATCCCATGTTGTTAATGAGCTCCATGTACCACTTCTATCATTTGGAGCAACAGACCCCTCCCTTGCTGCTCTCCAGTACCCATATTTTGTCCGAACCACACAGAGTGACCATTTCCAAGTGTATGCAGTTGCTGATTTAGTTGAGTATTTTGGATGGAGAGAAGTAATTGCAATCTTCGTAGACGATGATTATGGCAGGAATGGGATTGCTATACTAGGTGATGCCTTGGCGAAGAAGCGTTCCAAGATCTCATACAAGGCTGCCTTTTCTCCTGGAGCCTCCAAAAGTGATATCAGTGAATTGTTGGTGGGAGTGAACCTCATGGAATCTCGCGTTTATATTGTTCATGTTAATCCTGACTCCGGTTTAACAATTTTTTCCATTGCCAAGGCACTTGGAATGATGACCAGTGGCTATGTTTGGATTGCAACGGATTGGCTTCCTTCTCATTTAGATTCTCTAAATCCCCCTGGCCCCGACACAATGAATCTCTTGCAAGGGGTTGTTGCTCTTCGTCATCATACCCCAGATACCGATCTCAAAAAGCGCTTTATGTCTAGATGGAGCAAACTGAAACATGAAGGTAGTCCAAGCTTCAATTCTTATGCGCTCTATGCGTATGACTCTGTTTGGTTAGCTGCCCGTGCGCTTGATGATTTTTTCAATGAAGGTGGGAAAGTATCTTTCTCTGATGACCCAAGGTTGAAAGACACCAATAGAAGCACACTGCACTTAACATCACTCCGTATTTTTGATGGGGGCCAAAAATATCTACAGACGATTCTTAAGACGAACTTCACAGGTGTAAGTGGTCAGATTGAGTTTGATCAGGATAAATATCTTGTTCGTCCAGCATATGACATTCTGAATATTGGAGGAACTGGTTCCCGTAGAATTGGCTATTGGTCAAATTCTACTGGTCTCTCAGTCATTGCTCCTGAGATATTATATAAGAAGCCATTCAATAAGAATACCACTGCTCAACTTTATAGCGTTATATGGCCTGGTGAAGTTACAGCTACACCTCGTGGATGGGTGTTTCCCAACAATGGCAGGCCACTTCGAATTGGAGTACCTTATCGGGTAAGTTACCAAGATTTTGTGGCTAAAGACAAGAGCCCTCCAGGTGTCAGAGGATACTGTATTGATGTCTTTGAAGCTGCTGTAAACTTGTTGCCATATGCTGTGCCAAGGACGTATGTGTTGTATGGAAATGGTAAGAGGAATCCTGAGTACAGCGATCTTGTATTCCAGGTTGCGCAAAATAACTTTGATGCAGCTGTTGGAGATGTTACAATTACTACTAATAGGACAAGAATAGTTGATTTTACACAGCCTTACATGGAATCGGGACTTGTCGTAGTTGTTCCTGTCAAACAGGCAAAATCAAAACCTTGGGCTTTCCTCAAGCCATTTACATATCAAATGTGGTTGGTCACCGGCGCCTTCTTCCTTTTTGTGGGAGTTGTTGTTTGGATTCTTGAGCACCGGATGAATCACGAGTTCCGTGGTCCACCGAGGCAACAGCTCATAACGATTTGTTGGTTTAGTTTCTCCACAATGTTTTTCTCACACAGAGAGAACACTGTGAGCACCCTGGGACGGCTGGTGCTGGTTATATGGTTGTTTGTAGTGTTAATTATCAATTCCAGCTACACCGCTAGTTTGACTTCAATTCTCACGGTGCAGCAGCTGACATCACGGATTGAAGGGATTAATAGCTTGGTATCAAGTAATGATCCAATCGGAGTTCAAGACGGGTCCTTCATTTGGAAGTATTTGGTTGATGAGCTAAACATAGCAGAATCTAGACTCGTTAAGTTGAAAGACATGGAAGCCTATATTAAAGCCCTTACGGATGGACCAAGACGTGGTGGGGTAGCTGCCATCGTTGATGAGCTTCCTTACATTGAGTTGTTTATGTCCAGCACCAAATGCGCATTCAGGACTGTGGGGCAGGAGTTTACCAAAAGCGGATGGGGATTCGCCTTCCAAAGGGATTCTCCTCTTGCTGTCGACTTGTCGACAGCGATTCTTCAACTATCAGAGAATGGTGATCTCCAAAAGATACATAATAAGTGGCTTACGCAGAACGAGTGCTCTATTCAAATCAATGACGATGAAGACGACCGGCTGTCTCTAACAAGCTTTTGGGGCCTGTTTCTTATCTGCGGCATTGCATGCTTCCTTGCTCTTGCGGTTTTCTTCTGCAGAATCCTTTTTCAATACCGCAGATTTACTCCAGAGCCTGTGGAAGTGGACGTTGAGGAGATTGGACCTCCAAACCCAAGATCTAGACGCTCACTCCGGTCAACTAGTTTCAAGGATCTTATTGATTTTGTAGATAAGAAAGAAACTGAGATCAAGCACATGCTTAAGCGAAAGAGTAATGATGGGAAGGACGAAGCTAGCCCCAGCACTGATCGGCAGCCCCGTTCGCGCTCGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0004888 GO:0005215 GO:0005216 GO:0005261 GO:0005262 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0006950 GO:0007154 GO:0007165 GO:0008066 GO:0008150 GO:0008324 GO:0009266 GO:0009409 GO:0009507 GO:0009536 GO:0009605 GO:0009611 GO:0009612 GO:0009628 GO:0009719 GO:0009987 GO:0010033 GO:0010034 GO:0010243 GO:0015075 GO:0015085 GO:0015267 GO:0015276 GO:0015318 GO:0016020 GO:0019722 GO:0019932 GO:0022803 GO:0022834 GO:0022836 GO:0022838 GO:0022839 GO:0022857 GO:0022890 GO:0023052 GO:0030001 GO:0033554 GO:0034220 GO:0035556 GO:0038023 GO:0042221 GO:0043200 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0046677 GO:0046873 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051716 GO:0055085 GO:0060089 GO:0065007 GO:0070417 GO:0070588 GO:0070838 GO:0070887 GO:0071214 GO:0071229 GO:0071230 GO:0071236 GO:0071260 GO:0071310 GO:0071311 GO:0071417 GO:0071495 GO:0071496 GO:0071944 GO:0072511 GO:0098655 GO:0098660 GO:0098662 GO:0104004 GO:1901698 GO:1901699 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ANF_receptor Lig_chan SBP_bac_3

Protein Analysis

940

Amino Acids

105.11

Weight (kDa)

7.91

Isoelectric Point (pI)

40.28

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peripla_BP_6	PF13458	45 - 342	7.7e-21	Periplasmic binding protein
ANF_receptor	PF01094	60 - 417	7.8e-93	Receptor family ligand binding region
Lig_chan-Glu_bd	PF10613	499 - 582	2.7e-11	Ligated ion channel L-glutamate- and glycine-binding site
SBP_bac_3	PF00497	536 - 820	2.2e-11	Bacterial extracellular solute-binding proteins, family 3
Lig_chan	PF00060	598 - 852	1.9e-39	Ligand-gated ion channel

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001061)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G05200 AT1G05200 AT1G05200 AT1G05200 AT1G05200 AT1G05200 AT1G05200 AT2G32390 AT2G32390 AT2G32390 AT2G32390 AT2G32390 AT2G32390 AT2G32390
fragaria_vesca	FvH4_6g43291 FvH4_6g43291 FvH4_6g43291 FvH4_6g43291 FvH4_6g43291 FvH4_6g43291 FvH4_6g43291 FvH4_6g43291 FvH4_6g43291 FvH4_6g43291
malus_domestica	MD09G1069900.v1.1 MD09G1070100.v1.1 MD09G1107700.v1.1 MD09G1108100.v1.1 MD17G1096100.v1.1
prunus_persica	Prupe.3G217900_v2.0.a1 Prupe.3G217900_v2.0.a1 Prupe.3G217900_v2.0.a1 Prupe.3G217900_v2.0.a1 Prupe.3G217900_v2.0.a1 Prupe.3G217900_v2.0.a1 Prupe.3G217900_v2.0.a1 Prupe.3G217900_v2.0.a1
pyrus_communis	pycom09g03000 pycom09g03030 pycom09g03050 pycom09g03070 pycom15g20430 pycom17g09220
rosa_chinensis	RchiOBHm_Chr1g0324131 RchiOBHm_Chr2g0159571 RchiOBHm_Chr2g0159581
rosa_laevigata	RLG00000021167
rosa_multiflora	Rmu_co8397097.1_g000001 Rmu_sc0004966.1_g000017 Rmu_sc0020031.1_g000001
rosa_roxburghii	Rroxscaffold_2G00090390
rosa_rugosa	Rorug02G0476900 Rorug02G0477000 Rorug02G0477100 Rorug02G0477200
rosa_samantha	Rh2AG544200 Rh2BG557000 Rh2CG527200 Rh2DG566000
rosa_wichuraiana	Rw2G044990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	2 cut(s)	1632, 2311
AccB1I	GGYRCC	1 cut(s)	1814
AccB7I	CCANNNNNTGG	1 cut(s)	2243
AccI	GTMKAC	3 cut(s)	552, 2388, 2396
AccII	CGCG	3 cut(s)	237, 705, 2812
AccIII	TCCGGA	1 cut(s)	353
AciI	CCGC	9 cut(s)	176, 237, 279, 344, 2031, 2346, 2544, 2570, 2602
AclI	AACGTT	1 cut(s)	252
AclWI	GGATC	2 cut(s)	2105, 2706
AcoI	YGGCCR	1 cut(s)	177
AcsI	RAATTY	1 cut(s)	1282
AcyI	GRCGYC	1 cut(s)	1815
AdeI	CACNNNGTG	2 cut(s)	476, 1400
AfaI	GTAC	5 cut(s)	127, 399, 449, 1446, 1607
AfeI	AGCGCT	1 cut(s)	920
AfiI	CCNNNNNNNGG	7 cut(s)	734, 1421, 1831, 1974, 2142, 2243, 2367
AflII	CTTAAG	2 cut(s)	1157, 2747
AhdI	GACNNNNNGTC	1 cut(s)	2506
AhlI	ACTAGT	1 cut(s)	2687
AjiI	CACGTC	1 cut(s)	2243
AjnI	CCWGG	6 cut(s)	643, 841, 1375, 1495, 1623, 1968
AjuI	GAANNNNNNNTTGG	6 cut(s)	955, 987, 1258, 1290, 2297, 2329
Alw21I	GWGCWC	4 cut(s)	393, 1860, 1967, 2474
Alw26I	GTCTC	3 cut(s)	73, 1298, 2514
AlwI	GGATC	2 cut(s)	2105, 2706
AlwNI	CAGNNNCTG	3 cut(s)	503, 2065, 2621
Aor13HI	TCCGGA	1 cut(s)	353
Aor51HI	AGCGCT	1 cut(s)	920
AoxI	GGCC	9 cut(s)	11, 119, 177, 340, 844, 1132, 1373, 1427, 2527
ApoI	RAATTY	1 cut(s)	1282
AseI	ATTAAT	1 cut(s)	2088
Asp700I	GAANNNNTTC	2 cut(s)	1255, 1331
AspLEI	GCGC	8 cut(s)	145, 921, 982, 1021, 1633, 1817, 2312, 2814
AspS9I	GGNCC	8 cut(s)	341, 845, 1132, 1883, 2133, 2234, 2527, 2648
AsuHPI	GGTGA	4 cut(s)	602, 1391, 1801, 2438
AsuII	TTCGAA	1 cut(s)	1435
AsuNHI	GCTAGC	1 cut(s)	2780
AvaII	GGWCC	4 cut(s)	1883, 2133, 2234, 2648
BanI	GGYRCC	1 cut(s)	1814
BanII	GRGCYC	2 cut(s)	393, 1493
BauI	CACGAG	3 cut(s)	1398, 1871, 2815
BbsI	GAAGAC	1 cut(s)	2502
Bbv12I	GWGCWC	4 cut(s)	393, 1860, 1967, 2474
BccI	CCATC	9 cut(s)	76, 519, 927, 1121, 1398, 2225, 2267, 2343, 2759
BceAI	ACGGC	1 cut(s)	1991
BcgI	CGANNNNNNTGC	2 cut(s)	311, 345
BciT130I	CCWGG	6 cut(s)	645, 843, 1377, 1497, 1625, 1970
BciVI	GTATCC	1 cut(s)	1502
BclI	TGATCA	2 cut(s)	220, 1198
BcoDI	GTCTC	3 cut(s)	73, 1298, 2514
BcuI	ACTAGT	1 cut(s)	2687
BfaI	CTAG	8 cut(s)	15, 587, 930, 2034, 2181, 2667, 2688, 2781
BfmI	CTRYAG	3 cut(s)	305, 1145, 2581
BfoI	RGCGCY	3 cut(s)	146, 922, 1818
BfrI	CTTAAG	2 cut(s)	1157, 2747
BfuI	GTATCC	1 cut(s)	1502
BglII	AGATCT	2 cut(s)	618, 2663
BmcAI	AGTACT	1 cut(s)	127
Bme1390I	CCNGG	6 cut(s)	645, 843, 1377, 1497, 1625, 1970
Bme18I	GGWCC	4 cut(s)	1883, 2133, 2234, 2648
BmeRI	GACNNNNNGTC	1 cut(s)	2506
BmgBI	CACGTC	1 cut(s)	2243
BmgT120I	GGNCC	8 cut(s)	341, 845, 1132, 1883, 2133, 2234, 2527, 2648
BmiI	GGNNCC	7 cut(s)	649, 847, 1133, 1258, 1816, 2134, 2528
BmrFI	CCNGG	6 cut(s)	645, 843, 1377, 1497, 1625, 1970
BmsI	GCATC	2 cut(s)	583, 1636
BmtI	GCTAGC	1 cut(s)	2784
BpiI	GAAGAC	1 cut(s)	2502
BplI	GAGNNNNNCTC	2 cut(s)	675, 707
BpmI	CTGGAG	5 cut(s)	28, 428, 666, 1479, 2598
Bpu14I	TTCGAA	1 cut(s)	1435
BpuEI	CTTGAG	2 cut(s)	1763, 1874
BsaBI	GATNNNNATC	2 cut(s)	906, 1209
BsaHI	GRCGYC	1 cut(s)	1815
BsaI	GGTCTC	2 cut(s)	73, 1298
BsaWI	WCCGGW	4 cut(s)	353, 734, 1860, 2679
BsaXI	ACNNNNNCTCC	4 cut(s)	1827, 1857, 2661, 2691
Bsc4I	CCNNNNNNNGG	7 cut(s)	734, 1421, 1831, 1974, 2142, 2243, 2367
Bse118I	RCCGGY	2 cut(s)	1811, 2502
Bse1I	ACTGG	5 cut(s)	11, 445, 780, 1258, 1294
Bse3DI	GCAATG	3 cut(s)	753, 1302, 2547
Bse8I	GATNNNNATC	2 cut(s)	906, 1209
BseAI	TCCGGA	1 cut(s)	353
BseBI	CCWGG	6 cut(s)	645, 843, 1377, 1497, 1625, 1970
BseGI	GGATG	6 cut(s)	214, 530, 1409, 1869, 2236, 2354
BseJI	GATNNNNATC	2 cut(s)	906, 1209
BseLI	CCNNNNNNNGG	7 cut(s)	734, 1421, 1831, 1974, 2142, 2243, 2367
BseMI	GCAATG	3 cut(s)	753, 1302, 2547
BseMII	CTCAG	4 cut(s)	1302, 1311, 1593, 2721
BseNI	ACTGG	5 cut(s)	11, 445, 780, 1258, 1294
BseRI	GAGGAG	2 cut(s)	2367, 2654
BseYI	CCCAGC	3 cut(s)	43, 86, 2786
BsgI	GTGCAG	2 cut(s)	1085, 2078
Bsh1236I	CGCG	3 cut(s)	237, 705, 2812
Bsh1285I	CGRYCG	1 cut(s)	2503
BshFI	GGCC	9 cut(s)	13, 121, 179, 342, 846, 1134, 1375, 1429, 2529
BshNI	GGYRCC	1 cut(s)	1814
BsiEI	CGRYCG	1 cut(s)	2503
BsiHKAI	GWGCWC	4 cut(s)	393, 1860, 1967, 2474
BsiSI	CCGG	6 cut(s)	354, 735, 1812, 1861, 2503, 2680
BslFI	GGGAC	4 cut(s)	103, 253, 1737, 1986
BslI	CCNNNNNNNGG	7 cut(s)	734, 1421, 1831, 1974, 2142, 2243, 2367
BsmAI	GTCTC	3 cut(s)	73, 1298, 2514
BsmFI	GGGAC	4 cut(s)	103, 253, 1737, 1986
BsmI	GAATGC	1 cut(s)	2312
BsnI	GGCC	9 cut(s)	13, 121, 179, 342, 846, 1134, 1375, 1429, 2529
Bso31I	GGTCTC	2 cut(s)	73, 1298
Bsp119I	TTCGAA	1 cut(s)	1435
Bsp1286I	GDGCHC	5 cut(s)	393, 1493, 1860, 1967, 2474
Bsp13I	TCCGGA	1 cut(s)	353
Bsp19I	CCATGG	1 cut(s)	72
BspACI	CCGC	9 cut(s)	176, 237, 279, 344, 2031, 2346, 2544, 2570, 2602
BspANI	GGCC	9 cut(s)	13, 121, 179, 342, 846, 1134, 1375, 1429, 2529
BspCNI	CTCAG	4 cut(s)	1303, 1310, 1594, 2722
BspEI	TCCGGA	1 cut(s)	353
BspFNI	CGCG	3 cut(s)	237, 705, 2812
BspLI	GGNNCC	7 cut(s)	649, 847, 1133, 1258, 1816, 2134, 2528
BspMAI	CTGCAG	2 cut(s)	309, 2585
BspOI	GCTAGC	1 cut(s)	2784
BspPI	GGATC	2 cut(s)	2105, 2706
BspQI	GCTCTTC	1 cut(s)	888
BspT104I	TTCGAA	1 cut(s)	1435
BspT107I	GGYRCC	1 cut(s)	1814
BspTI	CTTAAG	2 cut(s)	1157, 2747
BspTNI	GGTCTC	2 cut(s)	73, 1298
BsrDI	GCAATG	3 cut(s)	753, 1302, 2547
BsrFI	RCCGGY	2 cut(s)	1811, 2502
BsrI	ACTGG	5 cut(s)	11, 445, 780, 1258, 1294
BssAI	RCCGGY	2 cut(s)	1811, 2502
BssNI	GRCGYC	1 cut(s)	1815
BssSI	CACGAG	3 cut(s)	1398, 1871, 2815
BssT1I	CCWWGG	6 cut(s)	72, 597, 759, 1070, 1562, 1766
Bst2BI	CACGAG	3 cut(s)	1398, 1871, 2815
Bst2UI	CCWGG	6 cut(s)	645, 843, 1377, 1497, 1625, 1970
Bst4CI	ACNGT	5 cut(s)	295, 1514, 1960, 2056, 2323
Bst6I	CTCTTC	1 cut(s)	888
BstACI	GRCGYC	1 cut(s)	1815
BstAFI	CTTAAG	2 cut(s)	1157, 2747
BstAPI	GCANNNNNTGC	2 cut(s)	2307, 2553
BstBI	TTCGAA	1 cut(s)	1435
BstC8I	GCNNGC	5 cut(s)	186, 344, 1427, 2554, 2782
BstDEI	CTNAG	4 cut(s)	1297, 1311, 1602, 2730
BstDSI	CCRYGG	2 cut(s)	72, 1879
BstEII	GGTNACC	1 cut(s)	1807
BstENI	CCTNNNNNAGG	1 cut(s)	2365
BstF5I	GGATG	6 cut(s)	214, 530, 1409, 1869, 2236, 2354
BstFNI	CGCG	3 cut(s)	237, 705, 2812
BstH2I	RGCGCY	3 cut(s)	146, 922, 1818
BstHHI	GCGC	8 cut(s)	145, 921, 982, 1021, 1633, 1817, 2312, 2814
BstMAI	GTCTC	3 cut(s)	73, 1298, 2514
BstMCI	CGRYCG	1 cut(s)	2503
BstNI	CCWGG	6 cut(s)	645, 843, 1377, 1497, 1625, 1970
BstNSI	RCATGY	3 cut(s)	64, 2556, 2746
BstPI	GGTNACC	1 cut(s)	1807
BstSCI	CCNGG	6 cut(s)	643, 841, 1375, 1495, 1623, 1968
BstSFI	CTRYAG	3 cut(s)	305, 1145, 2581
BstUI	CGCG	3 cut(s)	237, 705, 2812
BstV2I	GAAGAC	1 cut(s)	2502
BstX2I	RGATCY	3 cut(s)	618, 2663, 2698
BstYI	RGATCY	3 cut(s)	618, 2663, 2698
BsuI	GTATCC	1 cut(s)	1502
BsuRI	GGCC	9 cut(s)	13, 121, 179, 342, 846, 1134, 1375, 1429, 2529
BtgI	CCRYGG	2 cut(s)	72, 1879
BtrI	CACGTC	1 cut(s)	2243
BtsCI	GGATG	6 cut(s)	214, 530, 1409, 1869, 2236, 2354
BtsI	GCAGTG	3 cut(s)	38, 1097, 1347
BtsIMutI	CAGTG	7 cut(s)	38, 673, 787, 1097, 1347, 1956, 2790
Cac8I	GCNNGC	5 cut(s)	186, 344, 1427, 2554, 2782
CaiI	CAGNNNCTG	3 cut(s)	503, 2065, 2621
CfoI	GCGC	8 cut(s)	145, 921, 982, 1021, 1633, 1817, 2312, 2814
Cfr10I	RCCGGY	2 cut(s)	1811, 2502
Cfr13I	GGNCC	8 cut(s)	341, 845, 1132, 1883, 2133, 2234, 2527, 2648
CseI	GACGC	1 cut(s)	2679
Csp6I	GTAC	5 cut(s)	126, 398, 448, 1445, 1606
CspCI	CAANNNNNGTGG	2 cut(s)	1024, 1059
CviQI	GTAC	5 cut(s)	126, 398, 448, 1445, 1606
DdeI	CTNAG	4 cut(s)	1297, 1311, 1602, 2730
DinI	GGCGCC	1 cut(s)	1816
DraIII	CACNNNGTG	2 cut(s)	476, 1400
DriI	GACNNNNNGTC	1 cut(s)	2506
EaeI	YGGCCR	1 cut(s)	177
Eam1104I	CTCTTC	1 cut(s)	888
Eam1105I	GACNNNNNGTC	1 cut(s)	2506
EarI	CTCTTC	1 cut(s)	888
Ecl136II	GAGCTC	1 cut(s)	391
Eco130I	CCWWGG	6 cut(s)	72, 597, 759, 1070, 1562, 1766
Eco147I	AGGCCT	1 cut(s)	13
Eco24I	GRGCYC	2 cut(s)	393, 1493
Eco31I	GGTCTC	2 cut(s)	73, 1298
Eco32I	GATATC	1 cut(s)	664
Eco47I	GGWCC	4 cut(s)	1883, 2133, 2234, 2648
Eco47III	AGCGCT	1 cut(s)	920
Eco53kI	GAGCTC	1 cut(s)	391
Eco91I	GGTNACC	1 cut(s)	1807
EcoICRI	GAGCTC	1 cut(s)	391
EcoNI	CCTNNNNNAGG	1 cut(s)	2365
EcoO109I	RGGNCCY	2 cut(s)	2133, 2527
EcoO65I	GGTNACC	1 cut(s)	1807
EcoRII	CCWGG	6 cut(s)	643, 841, 1375, 1495, 1623, 1968
EcoRV	GATATC	1 cut(s)	664
EcoT14I	CCWWGG	6 cut(s)	72, 597, 759, 1070, 1562, 1766
EcoT38I	GRGCYC	2 cut(s)	393, 1493
EgeI	GGCGCC	1 cut(s)	1816
EheI	GGCGCC	1 cut(s)	1816
ErhI	CCWWGG	6 cut(s)	72, 597, 759, 1070, 1562, 1766
FalI	AAGNNNNNCTT	4 cut(s)	620, 652, 1151, 1183
FaqI	GGGAC	4 cut(s)	103, 253, 1737, 1986
FauI	CCCGC	2 cut(s)	230, 351
FauNDI	CATATG	2 cut(s)	1228, 1552
FbaI	TGATCA	2 cut(s)	220, 1198
FblI	GTMKAC	3 cut(s)	552, 2388, 2396
FokI	GGATG	6 cut(s)	221, 537, 1416, 1876, 2243, 2361
FriOI	GRGCYC	2 cut(s)	393, 1493
FspAI	RTGCGCAY	1 cut(s)	2311
FspBI	CTAG	8 cut(s)	15, 587, 930, 2034, 2181, 2667, 2688, 2781
FspI	TGCGCA	2 cut(s)	1632, 2311
GlaI	GCGC	8 cut(s)	144, 920, 981, 1020, 1632, 1816, 2311, 2813
GsaI	CCCAGC	3 cut(s)	47, 90, 2790
GsuI	CTGGAG	5 cut(s)	28, 428, 666, 1479, 2598
HaeII	RGCGCY	3 cut(s)	146, 922, 1818
HaeIII	GGCC	9 cut(s)	13, 121, 179, 342, 846, 1134, 1375, 1429, 2529
HapII	CCGG	6 cut(s)	354, 735, 1812, 1861, 2503, 2680
HgaI	GACGC	1 cut(s)	2679
HhaI	GCGC	8 cut(s)	145, 921, 982, 1021, 1633, 1817, 2312, 2814
Hin1I	GRCGYC	1 cut(s)	1815
Hin6I	GCGC	8 cut(s)	143, 919, 980, 1019, 1631, 1815, 2310, 2812
HinP1I	GCGC	8 cut(s)	143, 919, 980, 1019, 1631, 1815, 2310, 2812
HincII	GTYRAC	3 cut(s)	2389, 2397, 2685
HindII	GTYRAC	3 cut(s)	2389, 2397, 2685
HindIII	AAGCTT	2 cut(s)	964, 2518
HpaII	CCGG	6 cut(s)	354, 735, 1812, 1861, 2503, 2680
HphI	GGTGA	4 cut(s)	602, 1391, 1801, 2438
HpyAV	CCTTC	9 cut(s)	165, 825, 947, 1034, 1828, 2075, 2146, 2371, 2764
HpyCH4III	ACNGT	5 cut(s)	295, 1514, 1960, 2056, 2323
HpyCH4IV	ACGT	4 cut(s)	252, 1568, 2242, 2634
HpyF3I	CTNAG	4 cut(s)	1297, 1311, 1602, 2730
HpySE526I	ACGT	4 cut(s)	252, 1568, 2242, 2634
Hsp92I	GRCGYC	1 cut(s)	1815
HspAI	GCGC	8 cut(s)	143, 919, 980, 1019, 1631, 1815, 2310, 2812
KasI	GGCGCC	1 cut(s)	1814
Kpn2I	TCCGGA	1 cut(s)	353
Ksp22I	TGATCA	2 cut(s)	220, 1198
LguI	GCTCTTC	1 cut(s)	888
LmnI	GCTCC	5 cut(s)	396, 416, 647, 936, 1312
LweI	GCATC	2 cut(s)	583, 1636
MaeI	CTAG	8 cut(s)	15, 587, 930, 2034, 2181, 2667, 2688, 2781
MaeII	ACGT	4 cut(s)	252, 1568, 2242, 2634
MaeIII	GTNAC	5 cut(s)	476, 1384, 1460, 1663, 1807
MboII	GAAGA	8 cut(s)	224, 538, 616, 875, 1813, 2399, 2507, 2569
MfeI	CAATTG	2 cut(s)	201, 336
MflI	RGATCY	3 cut(s)	618, 2663, 2698
MhlI	GDGCHC	5 cut(s)	393, 1493, 1860, 1967, 2474
Mly113I	GGCGCC	1 cut(s)	1815
MlyI	GAGTC	3 cut(s)	725, 989, 2178
MmeI	TCCRAC	1 cut(s)	1636
MroI	TCCGGA	1 cut(s)	353
MroXI	GAANNNNTTC	2 cut(s)	1255, 1331
MslI	CAYNNNNRTG	1 cut(s)	77
MspA1I	CMGCKG	4 cut(s)	279, 310, 1652, 2065
MspCI	CTTAAG	2 cut(s)	1157, 2747
MspI	CCGG	6 cut(s)	354, 735, 1812, 1861, 2503, 2680
MspR9I	CCNGG	6 cut(s)	645, 843, 1377, 1497, 1625, 1970
MunI	CAATTG	2 cut(s)	201, 336
Mva1269I	GAATGC	1 cut(s)	2312
MvaI	CCWGG	6 cut(s)	645, 843, 1377, 1497, 1625, 1970
MvnI	CGCG	3 cut(s)	237, 705, 2812
NarI	GGCGCC	1 cut(s)	1815
NcoI	CCATGG	1 cut(s)	72
NdeI	CATATG	2 cut(s)	1228, 1552
NheI	GCTAGC	1 cut(s)	2780
NlaIV	GGNNCC	7 cut(s)	649, 847, 1133, 1258, 1816, 2134, 2528
NmeAIII	GCCGAG	1 cut(s)	147
NmuCI	GTSAC	2 cut(s)	476, 1807
NsbI	TGCGCA	2 cut(s)	1632, 2311
NspI	RCATGY	3 cut(s)	64, 2556, 2746
NspV	TTCGAA	1 cut(s)	1435
PaeI	GCATGC	1 cut(s)	2556
PasI	CCCWGGG	1 cut(s)	1969
PceI	AGGCCT	1 cut(s)	13
PciSI	GCTCTTC	1 cut(s)	888
PcsI	WCGNNNNNNNCGW	2 cut(s)	2496, 2803
PctI	GAATGC	1 cut(s)	2312
PdmI	GAANNNNTTC	2 cut(s)	1255, 1331
PflMI	CCANNNNNTGG	1 cut(s)	2243
PfoI	TCCNGGA	1 cut(s)	643
PleI	GAGTC	3 cut(s)	725, 989, 2178
PluTI	GGCGCC	1 cut(s)	1818
PpsI	GAGTC	3 cut(s)	725, 989, 2178
PpuMI	RGGWCCY	1 cut(s)	2133
PshBI	ATTAAT	1 cut(s)	2088
Psp124BI	GAGCTC	1 cut(s)	393
Psp1406I	AACGTT	1 cut(s)	252
Psp5II	RGGWCCY	1 cut(s)	2133
Psp6I	CCWGG	6 cut(s)	643, 841, 1375, 1495, 1623, 1968
PspEI	GGTNACC	1 cut(s)	1807
PspFI	CCCAGC	3 cut(s)	43, 86, 2786
PspGI	CCWGG	6 cut(s)	643, 841, 1375, 1495, 1623, 1968
PspN4I	GGNNCC	7 cut(s)	649, 847, 1133, 1258, 1816, 2134, 2528
PspPI	GGNCC	8 cut(s)	341, 845, 1132, 1883, 2133, 2234, 2527, 2648
PspPPI	RGGWCCY	1 cut(s)	2133
PstI	CTGCAG	2 cut(s)	309, 2585
PstNI	CAGNNNCTG	3 cut(s)	503, 2065, 2621
PsuI	RGATCY	3 cut(s)	618, 2663, 2698
PvuII	CAGCTG	3 cut(s)	310, 1652, 2065
RsaI	GTAC	5 cut(s)	127, 399, 449, 1446, 1607
RsaNI	GTAC	5 cut(s)	126, 398, 448, 1445, 1606
RseI	CAYNNNNRTG	1 cut(s)	77
SacI	GAGCTC	1 cut(s)	393
SalI	GTCGAC	2 cut(s)	2387, 2395
SapI	GCTCTTC	1 cut(s)	888
Sau96I	GGNCC	8 cut(s)	341, 845, 1132, 1883, 2133, 2234, 2527, 2648
ScaI	AGTACT	1 cut(s)	127
SchI	GAGTC	3 cut(s)	725, 989, 2178
ScrFI	CCNGG	6 cut(s)	645, 843, 1377, 1497, 1625, 1970
SduI	GDGCHC	5 cut(s)	393, 1493, 1860, 1967, 2474
SfaNI	GCATC	2 cut(s)	583, 1636
SfcI	CTRYAG	3 cut(s)	305, 1145, 2581
SfoI	GGCGCC	1 cut(s)	1816
SfuI	TTCGAA	1 cut(s)	1435
SgrAI	CRCCGGYG	1 cut(s)	1811
SinI	GGWCC	4 cut(s)	1883, 2133, 2234, 2648
SmiMI	CAYNNNNRTG	1 cut(s)	77
SmlI	CTYRAG	4 cut(s)	1157, 1778, 1853, 2747
SmoI	CTYRAG	4 cut(s)	1157, 1778, 1853, 2747
SpeI	ACTAGT	1 cut(s)	2687
SphI	GCATGC	1 cut(s)	2556
SseBI	AGGCCT	1 cut(s)	13
SsiI	CCGC	9 cut(s)	176, 237, 279, 344, 2031, 2346, 2544, 2570, 2602
SspDI	GGCGCC	1 cut(s)	1814
SspI	AATATT	2 cut(s)	136, 1243
SspMI	CTAG	8 cut(s)	15, 587, 930, 2034, 2181, 2667, 2688, 2781
SstI	GAGCTC	1 cut(s)	393
StuI	AGGCCT	1 cut(s)	13
StyD4I	CCNGG	6 cut(s)	643, 841, 1375, 1495, 1623, 1968
StyI	CCWWGG	6 cut(s)	72, 597, 759, 1070, 1562, 1766
TaaI	ACNGT	5 cut(s)	295, 1514, 1960, 2056, 2323
TaiI	ACGT	4 cut(s)	255, 1571, 2245, 2637
TaqI	TCGA	3 cut(s)	1435, 2388, 2396
TatI	WGTACW	2 cut(s)	125, 1605
TauI	GCSGC	2 cut(s)	179, 2547
TscAI	CASTG	7 cut(s)	45, 673, 787, 1104, 1354, 1963, 2797
TseFI	GTSAC	2 cut(s)	476, 1807
Tsp45I	GTSAC	2 cut(s)	476, 1807
TspDTI	ATGAA	8 cut(s)	17, 707, 872, 966, 1053, 1880, 2128, 2508
TspGWI	ACGGA	5 cut(s)	818, 1106, 1868, 2089, 2243
TspRI	CASTG	7 cut(s)	45, 673, 787, 1104, 1354, 1963, 2797
Van91I	CCANNNNNTGG	1 cut(s)	2243
Vha464I	CTTAAG	2 cut(s)	1157, 2747
VpaK11BI	GGWCC	4 cut(s)	1883, 2133, 2234, 2648
VspI	ATTAAT	1 cut(s)	2088
XagI	CCTNNNNNAGG	1 cut(s)	2365
XapI	RAATTY	1 cut(s)	1282
XbaI	TCTAGA	3 cut(s)	929, 2180, 2666
XceI	RCATGY	3 cut(s)	64, 2556, 2746
XcmI	CCANNNNNNNNNTGG	2 cut(s)	79, 164
XmiI	GTMKAC	3 cut(s)	552, 2388, 2396
XmnI	GAANNNNTTC	2 cut(s)	1255, 1331
XspI	CTAG	8 cut(s)	15, 587, 930, 2034, 2181, 2667, 2688, 2781
ZrmI	AGTACT	1 cut(s)	127

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.