pycom17g12030

CYSTM1 family protein A-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0001954
Physical Location & Seq
Forward (+)
9806224 .. 9807116
893 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 216 bp
ATGAGTTCTACTAAGACAGCAGCAGCATACCCCGCACTAGCTTCCTCTGCCGCACAGGGACCGTATCATGTCCAAGCTCCGCCACCTGCCGGCTACCCAACAAGAGATGAACCCCAAAACGCCGTTTCTGTAGAAACCAAGTCAAAGGGTGATGGCTTCTGGAAGGGCTGCTGCGCTGCCGTCTGTTGCTGTTGTATGCTGGATGCATGCTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CYSTM

Protein Analysis

72

Amino Acids

7.32

Weight (kDa)

6.5

Isoelectric Point (pI)

52.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CYSTM PF12734 31 - 71 3.1e-11 Cysteine-rich TM module stress tolerance
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 94
Acc36I ACCTGC 1 cut(s) 94
AciI CCGC 3 cut(s) 33, 51, 80
AfiI CCNNNNNNNGG 1 cut(s) 89
AluBI AGCT 2 cut(s) 41, 77
AluI AGCT 2 cut(s) 41, 77
ApeKI GCWGC 5 cut(s) 20, 23, 168, 171, 176
AspLEI GCGC 1 cut(s) 176
AspS9I GGNCC 1 cut(s) 59
AsuHPI GGTGA 1 cut(s) 161
AvaII GGWCC 1 cut(s) 59
BbvI GCAGC 5 cut(s) 32, 35, 155, 158, 163
BccI CCATC 1 cut(s) 146
BceAI ACGGC 2 cut(s) 107, 164
BfaI CTAG 1 cut(s) 38
BfmI CTRYAG 1 cut(s) 129
BfuAI ACCTGC 1 cut(s) 94
BisI GCNGC 6 cut(s) 21, 24, 51, 169, 172, 177
BlsI GCNGC 6 cut(s) 22, 25, 52, 170, 173, 178
Bme18I GGWCC 1 cut(s) 59
BmgT120I GGNCC 1 cut(s) 59
BmiI GGNNCC 1 cut(s) 60
BmsI GCATC 1 cut(s) 193
Bsc4I CCNNNNNNNGG 1 cut(s) 89
Bse118I RCCGGY 1 cut(s) 89
BseGI GGATG 1 cut(s) 208
BseLI CCNNNNNNNGG 1 cut(s) 89
BseXI GCAGC 5 cut(s) 32, 35, 155, 158, 163
BsiSI CCGG 1 cut(s) 90
BslFI GGGAC 1 cut(s) 72
BslI CCNNNNNNNGG 1 cut(s) 89
BsmFI GGGAC 1 cut(s) 72
BspACI CCGC 3 cut(s) 33, 51, 80
BspLI GGNNCC 1 cut(s) 60
BspMI ACCTGC 1 cut(s) 94
BsrFI RCCGGY 1 cut(s) 89
BssAI RCCGGY 1 cut(s) 89
Bst4CI ACNGT 1 cut(s) 63
BstC8I GCNNGC 2 cut(s) 91, 208
BstDEI CTNAG 1 cut(s) 12
BstF5I GGATG 1 cut(s) 208
BstHHI GCGC 1 cut(s) 176
BstMWI GCNNNNNNNGC 2 cut(s) 32, 47
BstNSI RCATGY 1 cut(s) 210
BstSFI CTRYAG 1 cut(s) 129
BstV1I GCAGC 5 cut(s) 32, 35, 155, 158, 163
BtsCI GGATG 1 cut(s) 208
BveI ACCTGC 1 cut(s) 94
Cac8I GCNNGC 2 cut(s) 91, 208
CfoI GCGC 1 cut(s) 176
Cfr10I RCCGGY 1 cut(s) 89
Cfr13I GGNCC 1 cut(s) 59
CviAII CATG 2 cut(s) 68, 207
CviJI RGCY 5 cut(s) 41, 77, 93, 156, 168
CviKI_1 RGCY 5 cut(s) 41, 77, 93, 156, 168
DdeI CTNAG 1 cut(s) 12
EciI GGCGGA 1 cut(s) 69
Eco47I GGWCC 1 cut(s) 59
EcoT22I ATGCAT 1 cut(s) 208
FaeI CATG 2 cut(s) 71, 210
FaiI YATR 4 cut(s) 28, 69, 197, 208
FaqI GGGAC 1 cut(s) 72
FatI CATG 2 cut(s) 67, 206
FauI CCCGC 1 cut(s) 40
Fnu4HI GCNGC 6 cut(s) 21, 24, 51, 169, 172, 177
Fsp4HI GCNGC 6 cut(s) 21, 24, 51, 169, 172, 177
FspBI CTAG 1 cut(s) 38
GlaI GCGC 1 cut(s) 175
GluI GCNGC 6 cut(s) 21, 24, 51, 169, 172, 177
HapII CCGG 1 cut(s) 90
HhaI GCGC 1 cut(s) 176
Hin1II CATG 2 cut(s) 71, 210
Hin6I GCGC 1 cut(s) 174
HinP1I GCGC 1 cut(s) 174
HpaII CCGG 1 cut(s) 90
HphI GGTGA 1 cut(s) 161
Hpy188III TCNNGA 1 cut(s) 160
HpyAV CCTTC 1 cut(s) 157
HpyCH4III ACNGT 1 cut(s) 63
HpyCH4V TGCA 1 cut(s) 206
HpyF10VI GCNNNNNNNGC 2 cut(s) 32, 47
HpyF3I CTNAG 1 cut(s) 12
Hsp92II CATG 2 cut(s) 71, 210
HspAI GCGC 1 cut(s) 174
KroI GCCGGC 1 cut(s) 89
KroNI GCCGGC 1 cut(s) 91
LmnI GCTCC 1 cut(s) 82
LpnPI CCDG 5 cut(s) 41, 99, 103, 145, 185
Lsp1109I GCAGC 5 cut(s) 32, 35, 155, 158, 163
LweI GCATC 1 cut(s) 193
MaeI CTAG 1 cut(s) 38
MnlI CCTC 1 cut(s) 55
Mph1103I ATGCAT 1 cut(s) 208
MroNI GCCGGC 1 cut(s) 89
MseI TTAA 1 cut(s) 214
MspI CCGG 1 cut(s) 90
MwoI GCNNNNNNNGC 2 cut(s) 32, 47
NaeI GCCGGC 1 cut(s) 91
NgoMIV GCCGGC 1 cut(s) 89
NlaIII CATG 2 cut(s) 71, 210
NlaIV GGNNCC 1 cut(s) 60
NsiI ATGCAT 1 cut(s) 208
NspI RCATGY 1 cut(s) 210
PaeI GCATGC 1 cut(s) 210
PaqCI CACCTGC 1 cut(s) 94
PdiI GCCGGC 1 cut(s) 91
PkrI GCNGC 6 cut(s) 22, 25, 52, 170, 173, 178
PspN4I GGNNCC 1 cut(s) 60
PspPI GGNCC 1 cut(s) 59
SaqAI TTAA 1 cut(s) 214
SatI GCNGC 6 cut(s) 21, 24, 51, 169, 172, 177
Sau96I GGNCC 1 cut(s) 59
SetI ASST 3 cut(s) 43, 79, 88
SfaNI GCATC 1 cut(s) 193
SfcI CTRYAG 1 cut(s) 129
SinI GGWCC 1 cut(s) 59
SphI GCATGC 1 cut(s) 210
SsiI CCGC 3 cut(s) 33, 51, 80
SspMI CTAG 1 cut(s) 38
TaaI ACNGT 1 cut(s) 63
TauI GCSGC 1 cut(s) 53
Tru1I TTAA 1 cut(s) 214
Tru9I TTAA 1 cut(s) 214
TseI GCWGC 5 cut(s) 20, 23, 168, 171, 176
TspDTI ATGAA 1 cut(s) 123
VpaK11BI GGWCC 1 cut(s) 59
XceI RCATGY 1 cut(s) 210
XspI CTAG 1 cut(s) 38
Zsp2I ATGCAT 1 cut(s) 208
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.