pycom17g24640

Bifunctional epoxide hydrolase 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0001763
Physical Location & Seq
Reverse (-)
22884108 .. 22885719
1612 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 942 bp
ATGTCACCTTGGACCCACCCCACAACATCATCTCATGAACCCGGATCCCCGCCGGATCCGGGAATCTGTCCCAAAGTGGTGCTGTTCTTGCATGGATTTCCAGAGATATGGTACACATGGAGGCATCAAATGGTTGCTGTCGCAGACAAAGGCTACCGGGCGATAGCCGTTGACTTCAGGGGCTTTGGGCTTTCCGAGCAGCCAGCTGAACCCGAAAAGGCAACCTTGAAAGATCTTGTTGACGATGTCGTTGACCTTCTTGATTCTTTGAGCATTAACAAGGTTGTTCTTGTTGGGAAGGATGTTGGAGCTCTGCCTGCATACCTAACACCTGCTCTCCACCCAGAGCGTGTATCCGGCATGATAACAGTAGGTGTGCCTTTCCTCAACCCGGCTAACTCTCCTGTCCGATTTGATCTCTTACCCGCAGGCTTCTACGTATTAAGATGGAAGGAGCCGGGGCGGGCAGAAGCAGATTTTGGCCGCTTTGATATCAAGACCGTGATACGAAACATCTACATTCTCTTCTCCGGGAGTGAGCTCCAAGTAGCCGCTCCTGATCAAGAAATCATGGACTTGGTTGACCCTGCCACTCCTCTACCACCATGGTTTTCGGAGGAAGATCTTGCAGTCTATGCATCTCTATACGAGAACTCTGGATTCCGTTATCCCATCCAAGTTACATACCAAACTAAGGTCGTGGATTACGGATTAAGCAGCGATCCAAAAGTGCCTGCTCCATCACTGCTTATCATGGGTGAGAAGGACTACTTCCTGAAATTTCCGGGAGTGGAAGACTACTTACGGACTGGAGCAGTGAAGCATTTTGTACCTGATTTGGACATTGCATATATTGCAGAAGGGAGCCACTTTGTGCACGAACAATTTCCTGATCAAGTTAATCAGCTCATCATCAGTTTCCTCGAAAAACATGGTATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000287 GO:0001676 GO:0003008 GO:0003013 GO:0003018 GO:0003674 GO:0003824 GO:0004301 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005782 GO:0005829 GO:0006082 GO:0006605 GO:0006625 GO:0006629 GO:0006631 GO:0006633 GO:0006690 GO:0006725 GO:0006793 GO:0006796 GO:0006805 GO:0006810 GO:0006873 GO:0006874 GO:0006875 GO:0006886 GO:0006950 GO:0006952 GO:0006954 GO:0006996 GO:0007031 GO:0008015 GO:0008104 GO:0008150 GO:0008152 GO:0008217 GO:0008610 GO:0009056 GO:0009058 GO:0009410 GO:0009636 GO:0009810 GO:0009893 GO:0009987 GO:0010468 GO:0010604 GO:0010628 GO:0015031 GO:0015643 GO:0015833 GO:0016043 GO:0016053 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0016801 GO:0016803 GO:0017144 GO:0018904 GO:0019216 GO:0019218 GO:0019222 GO:0019369 GO:0019373 GO:0019439 GO:0019725 GO:0019752 GO:0030003 GO:0030258 GO:0031907 GO:0031974 GO:0032501 GO:0032787 GO:0033036 GO:0033365 GO:0033559 GO:0034613 GO:0035150 GO:0035296 GO:0042221 GO:0042577 GO:0042578 GO:0042579 GO:0042592 GO:0042632 GO:0042759 GO:0042802 GO:0042803 GO:0042886 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043574 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0046272 GO:0046394 GO:0046483 GO:0046839 GO:0046872 GO:0046907 GO:0046983 GO:0048518 GO:0048878 GO:0050789 GO:0050801 GO:0050880 GO:0050896 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0051716 GO:0055065 GO:0055074 GO:0055080 GO:0055082 GO:0055088 GO:0055092 GO:0060255 GO:0062012 GO:0065007 GO:0065008 GO:0070013 GO:0070727 GO:0070887 GO:0071466 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072330 GO:0072503 GO:0072507 GO:0072593 GO:0072594 GO:0072662 GO:0072663 GO:0080090 GO:0090066 GO:0090181 GO:0097176 GO:0097746 GO:0097755 GO:0098771 GO:1900673 GO:1901360 GO:1901361 GO:1901568 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

314

Amino Acids

34.87

Weight (kDa)

4.89

Isoelectric Point (pI)

38.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 25 - 132 1.2e-10 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 26 - 143 3.1e-22 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 27 - 302 1.7e-16 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 340
Acc36I ACCTGC 1 cut(s) 340
AccBSI CCGCTC 1 cut(s) 554
AciI CCGC 5 cut(s) 50, 426, 463, 484, 552
AclWI GGATC 5 cut(s) 39, 50, 52, 63, 716
AcoI YGGCCR 1 cut(s) 481
AcsI RAATTY 1 cut(s) 779
AcuI CTGAAG 1 cut(s) 160
AdeI CACNNNGTG 1 cut(s) 874
AfaI GTAC 2 cut(s) 113, 831
AfiI CCNNNNNNNGG 3 cut(s) 59, 391, 694
AgsI TTSAA 1 cut(s) 229
AjuI GAANNNNNNNTTGG 2 cut(s) 462, 494
AluBI AGCT 4 cut(s) 206, 311, 541, 907
AluI AGCT 4 cut(s) 206, 311, 541, 907
Alw21I GWGCWC 3 cut(s) 313, 543, 879
Alw44I GTGCAC 1 cut(s) 875
AlwI GGATC 5 cut(s) 39, 50, 52, 63, 716
AoxI GGCC 1 cut(s) 481
ApaLI GTGCAC 1 cut(s) 875
ApeKI GCWGC 2 cut(s) 199, 717
ApoI RAATTY 1 cut(s) 779
Asp700I GAANNNNTTC 1 cut(s) 885
AspS9I GGNCC 1 cut(s) 12
AsuC2I CCSGG 7 cut(s) 42, 60, 158, 392, 459, 532, 786
AsuHPI GGTGA 1 cut(s) 770
AvaII GGWCC 1 cut(s) 12
BaeGI GKGCMC 1 cut(s) 879
BamHI GGATCC 2 cut(s) 44, 55
BanII GRGCYC 2 cut(s) 313, 543
BarI GAAGNNNNNNTAC 2 cut(s) 786, 818
BbsI GAAGAC 1 cut(s) 801
Bbv12I GWGCWC 3 cut(s) 313, 543, 879
BbvI GCAGC 2 cut(s) 211, 729
BccI CCATC 3 cut(s) 441, 680, 748
BceAI ACGGC 1 cut(s) 152
BciVI GTATCC 1 cut(s) 364
BclI TGATCA 2 cut(s) 559, 892
BcnI CCSGG 7 cut(s) 42, 60, 158, 392, 459, 532, 786
BfuAI ACCTGC 1 cut(s) 340
BfuI GTATCC 1 cut(s) 364
BglII AGATCT 2 cut(s) 232, 622
BisI GCNGC 4 cut(s) 200, 484, 552, 718
BlsI GCNGC 4 cut(s) 201, 485, 553, 719
Bme1390I CCNGG 7 cut(s) 42, 60, 158, 392, 459, 532, 786
Bme18I GGWCC 1 cut(s) 12
BmgT120I GGNCC 1 cut(s) 12
BmiI GGNNCC 5 cut(s) 14, 46, 57, 456, 866
BmrFI CCNGG 7 cut(s) 42, 60, 158, 392, 459, 532, 786
BmsI GCATC 2 cut(s) 133, 647
BpiI GAAGAC 1 cut(s) 801
BpmI CTGGAG 1 cut(s) 831
BpuMI CCSGG 7 cut(s) 42, 60, 158, 392, 459, 532, 786
BsaAI YACGTR 1 cut(s) 439
BsaJI CCNNGG 3 cut(s) 8, 458, 605
BsaXI ACNNNNNCTCC 2 cut(s) 321, 351
Bsc4I CCNNNNNNNGG 3 cut(s) 59, 391, 694
Bse1I ACTGG 1 cut(s) 814
Bse3DI GCAATG 1 cut(s) 843
BseDI CCNNGG 3 cut(s) 8, 458, 605
BseGI GGATG 2 cut(s) 307, 672
BseLI CCNNNNNNNGG 3 cut(s) 59, 391, 694
BseMI GCAATG 1 cut(s) 843
BseNI ACTGG 1 cut(s) 814
BseRI GAGGAG 1 cut(s) 585
BseSI GKGCMC 1 cut(s) 879
BseXI GCAGC 2 cut(s) 211, 729
BshFI GGCC 1 cut(s) 483
BsiHKAI GWGCWC 3 cut(s) 313, 543, 879
BsiSI CCGG 9 cut(s) 42, 53, 59, 157, 357, 392, 458, 531, 785
BslFI GGGAC 1 cut(s) 54
BslI CCNNNNNNNGG 3 cut(s) 59, 391, 694
BsmFI GGGAC 1 cut(s) 54
BsnI GGCC 1 cut(s) 483
Bsp1286I GDGCHC 3 cut(s) 313, 543, 879
Bsp143I GATC 8 cut(s) 44, 55, 232, 415, 559, 622, 721, 892
Bsp19I CCATGG 1 cut(s) 605
BspACI CCGC 5 cut(s) 50, 426, 463, 484, 552
BspANI GGCC 1 cut(s) 483
BspHI TCATGA 1 cut(s) 34
BspLI GGNNCC 5 cut(s) 14, 46, 57, 456, 866
BspMI ACCTGC 1 cut(s) 340
BspPI GGATC 5 cut(s) 39, 50, 52, 63, 716
BsrBI CCGCTC 1 cut(s) 554
BsrDI GCAATG 1 cut(s) 843
BsrI ACTGG 1 cut(s) 814
BssECI CCNNGG 3 cut(s) 8, 458, 605
BssMI GATC 8 cut(s) 44, 55, 232, 415, 559, 622, 721, 892
BssT1I CCWWGG 2 cut(s) 8, 605
Bst4CI ACNGT 2 cut(s) 370, 502
Bst6I CTCTTC 1 cut(s) 530
BstAPI GCANNNNNTGC 2 cut(s) 635, 854
BstBAI YACGTR 1 cut(s) 439
BstC8I GCNNGC 5 cut(s) 204, 318, 430, 465, 735
BstDEI CTNAG 1 cut(s) 693
BstDSI CCRYGG 1 cut(s) 605
BstF5I GGATG 2 cut(s) 307, 672
BstKTI GATC 8 cut(s) 47, 58, 235, 418, 562, 625, 724, 895
BstMBI GATC 8 cut(s) 44, 55, 232, 415, 559, 622, 721, 892
BstMWI GCNNNNNNNGC 5 cut(s) 88, 196, 317, 635, 854
BstSCI CCNGG 7 cut(s) 40, 58, 156, 390, 457, 530, 784
BstSLI GKGCMC 1 cut(s) 879
BstSNI TACGTA 1 cut(s) 439
BstV1I GCAGC 2 cut(s) 211, 729
BstV2I GAAGAC 1 cut(s) 801
BstX2I RGATCY 4 cut(s) 44, 55, 232, 622
BstXI CCANNNNNNTGG 1 cut(s) 108
BstYI RGATCY 4 cut(s) 44, 55, 232, 622
BsuI GTATCC 1 cut(s) 364
BsuRI GGCC 1 cut(s) 483
BtgI CCRYGG 1 cut(s) 605
BtsCI GGATG 2 cut(s) 307, 672
BtsI GCAGTG 2 cut(s) 743, 822
BtsIMutI CAGTG 2 cut(s) 743, 822
BveI ACCTGC 1 cut(s) 340
Cac8I GCNNGC 5 cut(s) 204, 318, 430, 465, 735
CciI TCATGA 1 cut(s) 34
Cfr13I GGNCC 1 cut(s) 12
Csp6I GTAC 2 cut(s) 112, 830
CviAII CATG 8 cut(s) 35, 92, 117, 361, 571, 606, 754, 932
CviQI GTAC 2 cut(s) 112, 830
DdeI CTNAG 1 cut(s) 693
DpnI GATC 8 cut(s) 46, 57, 234, 417, 561, 624, 723, 894
DpnII GATC 8 cut(s) 44, 55, 232, 415, 559, 622, 721, 892
DraIII CACNNNGTG 1 cut(s) 874
EaeI YGGCCR 1 cut(s) 481
Eam1104I CTCTTC 1 cut(s) 530
EarI CTCTTC 1 cut(s) 530
Ecl136II GAGCTC 2 cut(s) 311, 541
Eco105I TACGTA 1 cut(s) 439
Eco130I CCWWGG 2 cut(s) 8, 605
Eco24I GRGCYC 2 cut(s) 313, 543
Eco32I GATATC 1 cut(s) 493
Eco47I GGWCC 1 cut(s) 12
Eco53kI GAGCTC 2 cut(s) 311, 541
Eco57I CTGAAG 1 cut(s) 160
EcoICRI GAGCTC 2 cut(s) 311, 541
EcoRV GATATC 1 cut(s) 493
EcoT14I CCWWGG 2 cut(s) 8, 605
EcoT22I ATGCAT 1 cut(s) 640
EcoT38I GRGCYC 2 cut(s) 313, 543
ErhI CCWWGG 2 cut(s) 8, 605
FaeI CATG 8 cut(s) 38, 95, 120, 364, 574, 609, 757, 935
FalI AAGNNNNNCTT 4 cut(s) 209, 241, 755, 787
FaqI GGGAC 1 cut(s) 54
FatI CATG 8 cut(s) 34, 91, 116, 360, 570, 605, 753, 931
FauI CCCGC 3 cut(s) 57, 433, 456
FbaI TGATCA 2 cut(s) 559, 892
Fnu4HI GCNGC 4 cut(s) 200, 484, 552, 718
FokI GGATG 2 cut(s) 314, 659
FriOI GRGCYC 2 cut(s) 313, 543
Fsp4HI GCNGC 4 cut(s) 200, 484, 552, 718
GluI GCNGC 4 cut(s) 200, 484, 552, 718
GsuI CTGGAG 1 cut(s) 831
HaeIII GGCC 1 cut(s) 483
HapII CCGG 9 cut(s) 42, 53, 59, 157, 357, 392, 458, 531, 785
Hin1II CATG 8 cut(s) 38, 95, 120, 364, 574, 609, 757, 935
HincII GTYRAC 4 cut(s) 172, 241, 253, 583
HindII GTYRAC 4 cut(s) 172, 241, 253, 583
HinfI GANTC 3 cut(s) 63, 263, 660
HpaII CCGG 9 cut(s) 42, 53, 59, 157, 357, 392, 458, 531, 785
HphI GGTGA 1 cut(s) 770
Hpy166II GTNNAC 6 cut(s) 114, 172, 241, 253, 583, 877
Hpy188I TCNGA 3 cut(s) 196, 410, 616
Hpy188III TCNNGA 9 cut(s) 35, 101, 260, 496, 557, 563, 657, 775, 890
Hpy8I GTNNAC 6 cut(s) 114, 172, 241, 253, 583, 877
HpyAV CCTTC 5 cut(s) 266, 292, 445, 757, 854
HpyCH4III ACNGT 2 cut(s) 370, 502
HpyCH4IV ACGT 1 cut(s) 438
HpyCH4V TGCA 7 cut(s) 91, 320, 629, 638, 848, 857, 877
HpyF10VI GCNNNNNNNGC 5 cut(s) 88, 196, 317, 635, 854
HpyF3I CTNAG 1 cut(s) 693
HpySE526I ACGT 1 cut(s) 438
Hsp92II CATG 8 cut(s) 38, 95, 120, 364, 574, 609, 757, 935
Ksp22I TGATCA 2 cut(s) 559, 892
Kzo9I GATC 8 cut(s) 44, 55, 232, 415, 559, 622, 721, 892
LmnI GCTCC 7 cut(s) 308, 454, 546, 559, 742, 812, 864
Lsp1109I GCAGC 2 cut(s) 211, 729
LweI GCATC 2 cut(s) 133, 647
MaeII ACGT 1 cut(s) 438
MaeIII GTNAC 2 cut(s) 3, 679
MalI GATC 8 cut(s) 46, 57, 234, 417, 561, 624, 723, 894
MbiI CCGCTC 1 cut(s) 554
MboI GATC 8 cut(s) 44, 55, 232, 415, 559, 622, 721, 892
MboII GAAGA 3 cut(s) 517, 632, 806
MflI RGATCY 4 cut(s) 44, 55, 232, 622
MhlI GDGCHC 3 cut(s) 313, 543, 879
MluCI AATT 2 cut(s) 779, 884
MmeI TCCRAC 1 cut(s) 286
MnlI CCTC 5 cut(s) 114, 395, 606, 610, 932
Mph1103I ATGCAT 1 cut(s) 640
MroXI GAANNNNTTC 1 cut(s) 885
MseI TTAA 4 cut(s) 276, 443, 713, 900
MspA1I CMGCKG 1 cut(s) 206
MspI CCGG 9 cut(s) 42, 53, 59, 157, 357, 392, 458, 531, 785
MspR9I CCNGG 7 cut(s) 42, 60, 158, 392, 459, 532, 786
MwoI GCNNNNNNNGC 5 cut(s) 88, 196, 317, 635, 854
NciI CCSGG 7 cut(s) 42, 60, 158, 392, 459, 532, 786
NcoI CCATGG 1 cut(s) 605
NdeII GATC 8 cut(s) 44, 55, 232, 415, 559, 622, 721, 892
NlaIII CATG 8 cut(s) 38, 95, 120, 364, 574, 609, 757, 935
NlaIV GGNNCC 5 cut(s) 14, 46, 57, 456, 866
NmuCI GTSAC 1 cut(s) 3
NsiI ATGCAT 1 cut(s) 640
PagI TCATGA 1 cut(s) 34
PaqCI CACCTGC 1 cut(s) 340
PdmI GAANNNNTTC 1 cut(s) 885
PfeI GAWTC 3 cut(s) 63, 263, 660
PflFI GACNNNGTC 1 cut(s) 245
PfoI TCCNGGA 3 cut(s) 58, 530, 784
PkrI GCNGC 4 cut(s) 201, 485, 553, 719
Ppu21I YACGTR 1 cut(s) 439
Psp124BI GAGCTC 2 cut(s) 313, 543
PspN4I GGNNCC 5 cut(s) 14, 46, 57, 456, 866
PspPI GGNCC 1 cut(s) 12
PsuI RGATCY 4 cut(s) 44, 55, 232, 622
PsyI GACNNNGTC 1 cut(s) 245
PvuII CAGCTG 1 cut(s) 206
RsaI GTAC 2 cut(s) 113, 831
RsaNI GTAC 2 cut(s) 112, 830
SacI GAGCTC 2 cut(s) 313, 543
SaqAI TTAA 4 cut(s) 276, 443, 713, 900
SatI GCNGC 4 cut(s) 200, 484, 552, 718
Sau3AI GATC 8 cut(s) 44, 55, 232, 415, 559, 622, 721, 892
Sau96I GGNCC 1 cut(s) 12
ScrFI CCNGG 7 cut(s) 42, 60, 158, 392, 459, 532, 786
SduI GDGCHC 3 cut(s) 313, 543, 879
SfaNI GCATC 2 cut(s) 133, 647
SinI GGWCC 1 cut(s) 12
SnaBI TACGTA 1 cut(s) 439
Sse9I AATT 2 cut(s) 779, 884
SsiI CCGC 5 cut(s) 50, 426, 463, 484, 552
SstI GAGCTC 2 cut(s) 313, 543
StyD4I CCNGG 7 cut(s) 40, 58, 156, 390, 457, 530, 784
StyI CCWWGG 2 cut(s) 8, 605
TaaI ACNGT 2 cut(s) 370, 502
TaiI ACGT 1 cut(s) 441
TaqI TCGA 1 cut(s) 924
TasI AATT 2 cut(s) 779, 884
TauI GCSGC 2 cut(s) 486, 554
TfiI GAWTC 3 cut(s) 63, 263, 660
Tru1I TTAA 4 cut(s) 276, 443, 713, 900
Tru9I TTAA 4 cut(s) 276, 443, 713, 900
TscAI CASTG 2 cut(s) 750, 822
TseFI GTSAC 1 cut(s) 3
TseI GCWGC 2 cut(s) 199, 717
Tsp45I GTSAC 1 cut(s) 3
TspDTI ATGAA 1 cut(s) 51
TspGWI ACGGA 3 cut(s) 653, 723, 820
TspRI CASTG 2 cut(s) 750, 822
Tth111I GACNNNGTC 1 cut(s) 245
VneI GTGCAC 1 cut(s) 875
VpaK11BI GGWCC 1 cut(s) 12
XapI RAATTY 1 cut(s) 779
XmnI GAANNNNTTC 1 cut(s) 885
Zsp2I ATGCAT 1 cut(s) 640
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.