RchiOBHm_Chr7g0216591

Ribosome biogenesis protein NSA2 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0002786
Physical Location & Seq
Forward (+)
34201960 .. 34202605
646 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 447 bp
ATGATCACAAAAGCCACATTTATGGGACCTGGTTTTACAAGGAAACCTCCAAAATATGAGCCATTTATCCGGCCCAGTGGATTGCGAATCACTAAAGCTCATGTGACTCACCCTGAACTCAAATGTACTTTCAACCTCGAGATCATTGGTGTGAAGAAAAACCCTAATGGTCAAATGTATACCTCTTTTGGTGTCTTGACCAAGGGAACCATCATTGACCCATCTCAGTCTTGTGTGAATGTAAGTGAACTTGGTCTAGTCACACCTAACGGGAAAGTTGTGACAAATAATCCAAAGAATGATGCATGGATGCGTAAATGCAGTTCTACTTGTTTAAGAGGATTAACTGTCGAGCTTTCGGGTGACTCTTCTGATCTTGCATTGCACGCCTCTGCCTTATCATGCGATAGCCAACTAGCCTTCACCCAAAATATGGCTTTAATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribosomal_S8e

Protein Analysis

148

Amino Acids

16.06

Weight (kDa)

9.22

Isoelectric Point (pI)

37.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S8e PF01201 26 - 101 1.9e-17 Ribosomal protein S8e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 433
AccI GTMKAC 1 cut(s) 179
AfaI GTAC 1 cut(s) 127
AfiI CCNNNNNNNGG 1 cut(s) 433
AgsI TTSAA 1 cut(s) 133
AjnI CCWGG 1 cut(s) 28
AluBI AGCT 2 cut(s) 98, 355
AluI AGCT 2 cut(s) 98, 355
Ama87I CYCGRG 1 cut(s) 137
AoxI GGCC 1 cut(s) 71
AspS9I GGNCC 2 cut(s) 26, 72
AsuHPI GGTGA 3 cut(s) 101, 374, 415
AvaI CYCGRG 1 cut(s) 137
AvaII GGWCC 1 cut(s) 26
BccI CCATC 2 cut(s) 218, 229
BciT130I CCWGG 1 cut(s) 30
BclI TGATCA 1 cut(s) 3
BfaI CTAG 2 cut(s) 257, 416
Bme1390I CCNGG 1 cut(s) 30
Bme18I GGWCC 1 cut(s) 26
BmeT110I CYCGRG 1 cut(s) 137
BmgT120I GGNCC 2 cut(s) 26, 72
BmiI GGNNCC 2 cut(s) 27, 208
BmrFI CCNGG 1 cut(s) 30
BmrI ACTGGG 1 cut(s) 69
BmsI GCATC 2 cut(s) 292, 300
BmuI ACTGGG 1 cut(s) 69
BsaJI CCNNGG 1 cut(s) 201
Bsc4I CCNNNNNNNGG 1 cut(s) 433
Bse1I ACTGG 1 cut(s) 75
Bse3DI GCAATG 1 cut(s) 380
BseBI CCWGG 1 cut(s) 30
BseDI CCNNGG 1 cut(s) 201
BseGI GGATG 1 cut(s) 315
BseLI CCNNNNNNNGG 1 cut(s) 433
BseMI GCAATG 1 cut(s) 380
BseMII CTCAG 1 cut(s) 239
BseNI ACTGG 1 cut(s) 75
BshFI GGCC 1 cut(s) 73
BsiHKCI CYCGRG 1 cut(s) 137
BsiSI CCGG 1 cut(s) 70
BslFI GGGAC 1 cut(s) 39
BslI CCNNNNNNNGG 1 cut(s) 433
BsmFI GGGAC 1 cut(s) 39
BsnI GGCC 1 cut(s) 73
BsoBI CYCGRG 1 cut(s) 137
Bsp143I GATC 3 cut(s) 3, 141, 373
BspANI GGCC 1 cut(s) 73
BspCNI CTCAG 1 cut(s) 238
BspLI GGNNCC 2 cut(s) 27, 208
BsrDI GCAATG 1 cut(s) 380
BsrI ACTGG 1 cut(s) 75
BssECI CCNNGG 1 cut(s) 201
BssMI GATC 3 cut(s) 3, 141, 373
BssNAI GTATAC 1 cut(s) 180
BssT1I CCWWGG 1 cut(s) 201
Bst1107I GTATAC 1 cut(s) 180
Bst2UI CCWGG 1 cut(s) 30
Bst4CI ACNGT 1 cut(s) 349
Bst6I CTCTTC 1 cut(s) 373
BstC8I GCNNGC 1 cut(s) 387
BstDEI CTNAG 1 cut(s) 225
BstF5I GGATG 1 cut(s) 315
BstKTI GATC 3 cut(s) 6, 144, 376
BstMBI GATC 3 cut(s) 3, 141, 373
BstMWI GCNNNNNNNGC 1 cut(s) 386
BstNI CCWGG 1 cut(s) 30
BstSCI CCNGG 1 cut(s) 28
BstXI CCANNNNNNTGG 1 cut(s) 22
BstZ17I GTATAC 1 cut(s) 180
BsuRI GGCC 1 cut(s) 73
BtsCI GGATG 1 cut(s) 315
BtsIMutI CAGTG 1 cut(s) 82
Cac8I GCNNGC 1 cut(s) 387
Cfr13I GGNCC 2 cut(s) 26, 72
CsiI ACCWGGT 1 cut(s) 28
Csp6I GTAC 1 cut(s) 126
CviAII CATG 3 cut(s) 101, 306, 402
CviJI RGCY 8 cut(s) 14, 61, 73, 98, 355, 411, 419, 437
CviKI_1 RGCY 8 cut(s) 14, 61, 73, 98, 355, 411, 419, 437
CviQI GTAC 1 cut(s) 126
DdeI CTNAG 1 cut(s) 225
DpnI GATC 3 cut(s) 5, 143, 375
DpnII GATC 3 cut(s) 3, 141, 373
Eam1104I CTCTTC 1 cut(s) 373
EarI CTCTTC 1 cut(s) 373
Eco130I CCWWGG 1 cut(s) 201
Eco47I GGWCC 1 cut(s) 26
Eco88I CYCGRG 1 cut(s) 137
EcoO109I RGGNCCY 1 cut(s) 26
EcoRII CCWGG 1 cut(s) 28
EcoT14I CCWWGG 1 cut(s) 201
EcoT22I ATGCAT 1 cut(s) 307
ErhI CCWWGG 1 cut(s) 201
FaeI CATG 3 cut(s) 104, 309, 405
FaiI YATR 7 cut(s) 23, 57, 102, 180, 307, 403, 434
FaqI GGGAC 1 cut(s) 39
FatI CATG 3 cut(s) 100, 305, 401
FbaI TGATCA 1 cut(s) 3
FblI GTMKAC 1 cut(s) 179
FokI GGATG 1 cut(s) 322
FspBI CTAG 2 cut(s) 257, 416
HaeIII GGCC 1 cut(s) 73
HapII CCGG 1 cut(s) 70
Hin1II CATG 3 cut(s) 104, 309, 405
HinfI GANTC 3 cut(s) 87, 106, 365
HpaII CCGG 1 cut(s) 70
HphI GGTGA 3 cut(s) 101, 374, 415
Hpy166II GTNNAC 2 cut(s) 180, 248
Hpy188I TCNGA 1 cut(s) 373
Hpy188III TCNNGA 2 cut(s) 139, 196
Hpy8I GTNNAC 2 cut(s) 180, 248
HpyAV CCTTC 1 cut(s) 430
HpyCH4III ACNGT 1 cut(s) 349
HpyCH4V TGCA 4 cut(s) 305, 321, 380, 385
HpyF10VI GCNNNNNNNGC 1 cut(s) 386
HpyF3I CTNAG 1 cut(s) 225
Hsp92II CATG 3 cut(s) 104, 309, 405
Ksp22I TGATCA 1 cut(s) 3
Kzo9I GATC 3 cut(s) 3, 141, 373
LpnPI CCDG 5 cut(s) 15, 42, 83, 88, 126
LweI GCATC 2 cut(s) 292, 300
MabI ACCWGGT 1 cut(s) 28
MaeI CTAG 2 cut(s) 257, 416
MaeIII GTNAC 4 cut(s) 103, 259, 280, 362
MalI GATC 3 cut(s) 5, 143, 375
MboI GATC 3 cut(s) 3, 141, 373
MboII GAAGA 2 cut(s) 166, 360
MlyI GAGTC 2 cut(s) 100, 359
MnlI CCTC 5 cut(s) 57, 146, 193, 332, 400
Mph1103I ATGCAT 1 cut(s) 307
MseI TTAA 3 cut(s) 335, 344, 440
MslI CAYNNNNRTG 2 cut(s) 20, 149
MspI CCGG 1 cut(s) 70
MspR9I CCNGG 1 cut(s) 30
MvaI CCWGG 1 cut(s) 30
MwoI GCNNNNNNNGC 1 cut(s) 386
NdeII GATC 3 cut(s) 3, 141, 373
NlaIII CATG 3 cut(s) 104, 309, 405
NlaIV GGNNCC 2 cut(s) 27, 208
NmuCI GTSAC 4 cut(s) 103, 259, 280, 362
NsiI ATGCAT 1 cut(s) 307
PaeR7I CTCGAG 1 cut(s) 137
PfeI GAWTC 1 cut(s) 87
PflMI CCANNNNNTGG 1 cut(s) 433
PleI GAGTC 2 cut(s) 100, 359
PpsI GAGTC 2 cut(s) 100, 359
PpuMI RGGWCCY 1 cut(s) 26
Psp5II RGGWCCY 1 cut(s) 26
Psp6I CCWGG 1 cut(s) 28
PspGI CCWGG 1 cut(s) 28
PspN4I GGNNCC 2 cut(s) 27, 208
PspPI GGNCC 2 cut(s) 26, 72
PspPPI RGGWCCY 1 cut(s) 26
PsrI GAACNNNNNNTAC 2 cut(s) 307, 339
RsaI GTAC 1 cut(s) 127
RsaNI GTAC 1 cut(s) 126
RseI CAYNNNNRTG 2 cut(s) 20, 149
SaqAI TTAA 3 cut(s) 335, 344, 440
Sau3AI GATC 3 cut(s) 3, 141, 373
Sau96I GGNCC 2 cut(s) 26, 72
SchI GAGTC 2 cut(s) 100, 359
ScrFI CCNGG 1 cut(s) 30
SetI ASST 7 cut(s) 31, 49, 100, 138, 185, 268, 357
SexAI ACCWGGT 1 cut(s) 28
SfaNI GCATC 2 cut(s) 292, 300
Sfr274I CTCGAG 1 cut(s) 137
SinI GGWCC 1 cut(s) 26
SlaI CTCGAG 1 cut(s) 137
SmiMI CAYNNNNRTG 2 cut(s) 20, 149
SmlI CTYRAG 1 cut(s) 137
SmoI CTYRAG 1 cut(s) 137
SspMI CTAG 2 cut(s) 257, 416
StyD4I CCNGG 1 cut(s) 28
StyI CCWWGG 1 cut(s) 201
TaaI ACNGT 1 cut(s) 349
TaqI TCGA 2 cut(s) 138, 351
TatI WGTACW 1 cut(s) 125
TfiI GAWTC 1 cut(s) 87
Tru1I TTAA 3 cut(s) 335, 344, 440
Tru9I TTAA 3 cut(s) 335, 344, 440
TscAI CASTG 1 cut(s) 82
TseFI GTSAC 4 cut(s) 103, 259, 280, 362
Tsp45I GTSAC 4 cut(s) 103, 259, 280, 362
TspRI CASTG 1 cut(s) 82
Van91I CCANNNNNTGG 1 cut(s) 433
VpaK11BI GGWCC 1 cut(s) 26
XhoI CTCGAG 1 cut(s) 137
XmiI GTMKAC 1 cut(s) 179
XspI CTAG 2 cut(s) 257, 416
Zsp2I ATGCAT 1 cut(s) 307
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.