RchiOBHm_Chr2g0138051

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0004046
Physical Location & Seq
Reverse (-)
55849615 .. 55850436
822 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 822 bp
ATGGCGTCAGGCCACGAAGTCCAAGTGAAGCTCTCCTCCGCCAGAGACCTCAAGAACGTGAACTGGAGACACGGCCCCCTCAAGCCGTACGCCGTTGGGTGGGTCGAGCCGAGGAACAAGCTCTCCACCAAGGTGGACGAGGATGGCGACACGTCGCCCGAATGGGACGAGACGCTGACAATCCCTATCCCCGGTCCTGTCAACGACGACACCATGCTCCACATCGACATCGTCCATGCCGGAGCTGAGCCCGACACCAAACAGCTGGTGGGATCTGCTCGGCTCCGGCTCCGCGACGTTGTCGATGACGTCGGATATGAAGAACGCGCCAGCCGCACTCTTCACCTCAAAAGGCCTTCCGGTAGGCCACAGGGAAAGGTGGACGTCAAGGTTACAGTGAGGGAGCCGCGCTATCGTGTGCCGGAGCCGTATTATGTGCCGCCTTATGGGGTTCCACCGGCAGGCTCTGGAGGTTACCATGCTCCACCACCACCGCCGGCTTATGGCGACCCATACGCCGCACCACCACAGCCTCCGCCGGTGTATGGTGGAACGTACGCTGCACCGCCTTCCGGGTATCCCTACAATGCGACTCCGGTGGCGTACAGTCAGCCAGCTCAGCCAAGTTATGGTCAAGTGCAGTACGGGCAGGAGGAGAAGAAGAAGAGCAAGTTTGGTGGGATGGGAACGGGATTGGCTGTGGGGGCGGTAGCGGGGGCGCTGGGTGCATTTGCGTTGGCTGAGGGTGTTGATTATGTAGAGGACAAAATTGCAGACGACGTGGTGGATAATCTGAGCTACTATGATGGCGATGACTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

273

Amino Acids

29.44

Weight (kDa)

5.17

Isoelectric Point (pI)

39.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 6 - 104 1.9e-10 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 312, 387
AccB7I CCANNNNNTGG 1 cut(s) 629
AccII CGCG 3 cut(s) 294, 327, 409
AclWI GGATC 1 cut(s) 280
AcyI GRCGYC 3 cut(s) 5, 309, 384
AfaI GTAC 4 cut(s) 89, 557, 605, 644
AfiI CCNNNNNNNGG 8 cut(s) 99, 191, 446, 461, 503, 545, 572, 629
AflIII ACRYGT 1 cut(s) 150
AjiI CACGTC 2 cut(s) 153, 781
AleI CACNNNNGTG 1 cut(s) 131
AloI GAACNNNNNNTCC 2 cut(s) 107, 139
AluBI AGCT 6 cut(s) 31, 121, 245, 265, 617, 798
AluI AGCT 6 cut(s) 31, 121, 245, 265, 617, 798
Alw26I GTCTC 3 cut(s) 39, 61, 164
AlwI GGATC 1 cut(s) 280
AlwNI CAGNNNCTG 1 cut(s) 467
AoxI GGCC 4 cut(s) 10, 73, 353, 365
ApeKI GCWGC 1 cut(s) 560
AspLEI GCGC 3 cut(s) 329, 411, 721
AspS9I GGNCC 2 cut(s) 74, 194
AsuC2I CCSGG 2 cut(s) 192, 574
AsuHPI GGTGA 1 cut(s) 335
AvaII GGWCC 1 cut(s) 194
BanII GRGCYC 1 cut(s) 252
BbvCI CCTCAGC 1 cut(s) 741
BbvI GCAGC 1 cut(s) 547
BccI CCATC 3 cut(s) 137, 676, 800
BceAI ACGGC 4 cut(s) 70, 77, 88, 412
BciVI GTATCC 1 cut(s) 588
BcnI CCSGG 2 cut(s) 192, 574
BcoDI GTCTC 3 cut(s) 39, 61, 164
BfoI RGCGCY 1 cut(s) 722
BfuI GTATCC 1 cut(s) 588
BisI GCNGC 5 cut(s) 334, 407, 440, 519, 561
BlpI GCTNAGC 2 cut(s) 246, 618
BlsI GCNGC 5 cut(s) 335, 408, 441, 520, 562
Bme1390I CCNGG 2 cut(s) 192, 574
Bme18I GGWCC 1 cut(s) 194
BmgBI CACGTC 2 cut(s) 153, 781
BmgT120I GGNCC 2 cut(s) 74, 194
BmiI GGNNCC 6 cut(s) 76, 284, 290, 405, 426, 453
BmrFI CCNGG 2 cut(s) 192, 574
BpmI CTGGAG 2 cut(s) 85, 489
Bpu10I CCTNAGC 1 cut(s) 741
Bpu1102I GCTNAGC 2 cut(s) 246, 618
BpuEI CTTGAG 2 cut(s) 35, 65
BpuMI CCSGG 2 cut(s) 192, 574
BsaHI GRCGYC 3 cut(s) 5, 309, 384
BsaI GGTCTC 1 cut(s) 39
BsaJI CCNNGG 3 cut(s) 110, 129, 190
BsaWI WCCGGW 2 cut(s) 359, 595
BsaXI ACNNNNNCTCC 2 cut(s) 107, 137
Bsc4I CCNNNNNNNGG 8 cut(s) 99, 191, 446, 461, 503, 545, 572, 629
Bse118I RCCGGY 3 cut(s) 457, 496, 538
Bse1I ACTGG 1 cut(s) 68
BseDI CCNNGG 3 cut(s) 110, 129, 190
BseGI GGATG 2 cut(s) 148, 687
BseLI CCNNNNNNNGG 8 cut(s) 99, 191, 446, 461, 503, 545, 572, 629
BseMII CTCAG 4 cut(s) 237, 632, 732, 785
BseNI ACTGG 1 cut(s) 68
BseRI GAGGAG 2 cut(s) 25, 668
BseXI GCAGC 1 cut(s) 547
BseYI CCCAGC 1 cut(s) 721
BsgI GTGCAG 2 cut(s) 546, 659
Bsh1236I CGCG 3 cut(s) 294, 327, 409
BshFI GGCC 4 cut(s) 12, 75, 355, 367
BsiWI CGTACG 2 cut(s) 87, 555
BslFI GGGAC 1 cut(s) 179
BslI CCNNNNNNNGG 8 cut(s) 99, 191, 446, 461, 503, 545, 572, 629
BsmAI GTCTC 3 cut(s) 39, 61, 164
BsmBI CGTCTC 1 cut(s) 164
BsmFI GGGAC 1 cut(s) 179
BsnI GGCC 4 cut(s) 12, 75, 355, 367
Bso31I GGTCTC 1 cut(s) 39
Bsp1286I GDGCHC 1 cut(s) 252
Bsp143I GATC 1 cut(s) 272
Bsp1720I GCTNAGC 2 cut(s) 246, 618
BspANI GGCC 4 cut(s) 12, 75, 355, 367
BspCNI CTCAG 4 cut(s) 238, 631, 733, 786
BspFNI CGCG 3 cut(s) 294, 327, 409
BspLI GGNNCC 6 cut(s) 76, 284, 290, 405, 426, 453
BspPI GGATC 1 cut(s) 280
BspQI GCTCTTC 1 cut(s) 659
BspTNI GGTCTC 1 cut(s) 39
BsrFI RCCGGY 3 cut(s) 457, 496, 538
BsrI ACTGG 1 cut(s) 68
BssAI RCCGGY 3 cut(s) 457, 496, 538
BssECI CCNNGG 3 cut(s) 110, 129, 190
BssMI GATC 1 cut(s) 272
BssNI GRCGYC 3 cut(s) 5, 309, 384
BssT1I CCWWGG 1 cut(s) 129
Bst4CI ACNGT 2 cut(s) 397, 608
Bst6I CTCTTC 2 cut(s) 345, 659
BstACI GRCGYC 3 cut(s) 5, 309, 384
BstC8I GCNNGC 4 cut(s) 331, 463, 498, 615
BstDEI CTNAG 4 cut(s) 246, 618, 741, 794
BstEII GGTNACC 1 cut(s) 473
BstF5I GGATG 2 cut(s) 148, 687
BstFNI CGCG 3 cut(s) 294, 327, 409
BstH2I RGCGCY 1 cut(s) 722
BstHHI GCGC 3 cut(s) 329, 411, 721
BstKTI GATC 1 cut(s) 275
BstMAI GTCTC 3 cut(s) 39, 61, 164
BstMBI GATC 1 cut(s) 272
BstMWI GCNNNNNNNGC 5 cut(s) 333, 619, 646, 704, 725
BstPI GGTNACC 1 cut(s) 473
BstSCI CCNGG 2 cut(s) 190, 572
BstUI CGCG 3 cut(s) 294, 327, 409
BstV1I GCAGC 1 cut(s) 547
BstX2I RGATCY 1 cut(s) 272
BstXI CCANNNNNNTGG 2 cut(s) 133, 265
BstYI RGATCY 1 cut(s) 272
BsuI GTATCC 1 cut(s) 588
BsuRI GGCC 4 cut(s) 12, 75, 355, 367
BtrI CACGTC 2 cut(s) 153, 781
BtsCI GGATG 2 cut(s) 148, 687
BtsIMutI CAGTG 1 cut(s) 402
Cac8I GCNNGC 4 cut(s) 331, 463, 498, 615
CaiI CAGNNNCTG 1 cut(s) 467
CfoI GCGC 3 cut(s) 329, 411, 721
Cfr10I RCCGGY 3 cut(s) 457, 496, 538
Cfr13I GGNCC 2 cut(s) 74, 194
CseI GACGC 1 cut(s) 181
Csp6I GTAC 4 cut(s) 88, 556, 604, 643
CspCI CAANNNNNGTGG 2 cut(s) 658, 693
CviAII CATG 3 cut(s) 214, 236, 479
CviQI GTAC 4 cut(s) 88, 556, 604, 643
DdeI CTNAG 4 cut(s) 246, 618, 741, 794
DpnI GATC 1 cut(s) 274
DpnII GATC 1 cut(s) 272
Eam1104I CTCTTC 2 cut(s) 345, 659
EarI CTCTTC 2 cut(s) 345, 659
EciI GGCGGA 2 cut(s) 28, 525
Eco130I CCWWGG 1 cut(s) 129
Eco147I AGGCCT 1 cut(s) 355
Eco24I GRGCYC 1 cut(s) 252
Eco31I GGTCTC 1 cut(s) 39
Eco47I GGWCC 1 cut(s) 194
Eco91I GGTNACC 1 cut(s) 473
EcoO65I GGTNACC 1 cut(s) 473
EcoT14I CCWWGG 1 cut(s) 129
EcoT38I GRGCYC 1 cut(s) 252
ErhI CCWWGG 1 cut(s) 129
Esp3I CGTCTC 1 cut(s) 164
FaeI CATG 3 cut(s) 217, 239, 482
FaqI GGGAC 1 cut(s) 179
FatI CATG 3 cut(s) 213, 235, 478
FauI CCCGC 1 cut(s) 706
Fnu4HI GCNGC 5 cut(s) 334, 407, 440, 519, 561
FokI GGATG 2 cut(s) 155, 694
FriOI GRGCYC 1 cut(s) 252
Fsp4HI GCNGC 5 cut(s) 334, 407, 440, 519, 561
GlaI GCGC 3 cut(s) 328, 410, 720
GluI GCNGC 5 cut(s) 334, 407, 440, 519, 561
GsaI CCCAGC 1 cut(s) 725
GsuI CTGGAG 2 cut(s) 85, 489
HaeII RGCGCY 1 cut(s) 722
HaeIII GGCC 4 cut(s) 12, 75, 355, 367
HgaI GACGC 1 cut(s) 181
HhaI GCGC 3 cut(s) 329, 411, 721
Hin1I GRCGYC 3 cut(s) 5, 309, 384
Hin1II CATG 3 cut(s) 217, 239, 482
Hin6I GCGC 3 cut(s) 327, 409, 719
HinP1I GCGC 3 cut(s) 327, 409, 719
HincII GTYRAC 1 cut(s) 202
HindII GTYRAC 1 cut(s) 202
HinfI GANTC 1 cut(s) 592
HphI GGTGA 1 cut(s) 335
Hpy166II GTNNAC 4 cut(s) 61, 136, 202, 382
Hpy188I TCNGA 2 cut(s) 314, 795
Hpy188III TCNNGA 2 cut(s) 52, 468
Hpy8I GTNNAC 4 cut(s) 61, 136, 202, 382
Hpy99I CGWCG 5 cut(s) 157, 209, 299, 314, 782
HpyAV CCTTC 2 cut(s) 366, 579
HpyCH4III ACNGT 2 cut(s) 397, 608
HpyCH4IV ACGT 7 cut(s) 57, 152, 297, 309, 384, 554, 780
HpyCH4V TGCA 4 cut(s) 563, 640, 728, 773
HpyF10VI GCNNNNNNNGC 5 cut(s) 333, 619, 646, 704, 725
HpyF3I CTNAG 4 cut(s) 246, 618, 741, 794
HpySE526I ACGT 7 cut(s) 57, 152, 297, 309, 384, 554, 780
Hsp92I GRCGYC 3 cut(s) 5, 309, 384
Hsp92II CATG 3 cut(s) 217, 239, 482
HspAI GCGC 3 cut(s) 327, 409, 719
KroI GCCGGC 1 cut(s) 496
KroNI GCCGGC 1 cut(s) 498
Kzo9I GATC 1 cut(s) 272
LguI GCTCTTC 1 cut(s) 659
LmnI GCTCC 7 cut(s) 222, 242, 288, 294, 403, 424, 487
Lsp1109I GCAGC 1 cut(s) 547
MaeII ACGT 7 cut(s) 57, 152, 297, 309, 384, 554, 780
MaeIII GTNAC 2 cut(s) 391, 473
MalI GATC 1 cut(s) 274
MboI GATC 1 cut(s) 272
MboII GAAGA 5 cut(s) 332, 332, 670, 673, 676
MflI RGATCY 1 cut(s) 272
MhlI GDGCHC 1 cut(s) 252
MluCI AATT 1 cut(s) 768
MlyI GAGTC 1 cut(s) 586
MmeI TCCRAC 1 cut(s) 292
MroNI GCCGGC 1 cut(s) 496
MslI CAYNNNNRTG 1 cut(s) 131
MspA1I CMGCKG 1 cut(s) 265
MspR9I CCNGG 2 cut(s) 192, 574
MvnI CGCG 3 cut(s) 294, 327, 409
MwoI GCNNNNNNNGC 5 cut(s) 333, 619, 646, 704, 725
NaeI GCCGGC 1 cut(s) 498
NciI CCSGG 2 cut(s) 192, 574
NdeII GATC 1 cut(s) 272
NgoMIV GCCGGC 1 cut(s) 496
NlaIII CATG 3 cut(s) 217, 239, 482
NlaIV GGNNCC 6 cut(s) 76, 284, 290, 405, 426, 453
NmeAIII GCCGAG 2 cut(s) 135, 259
OliI CACNNNNGTG 1 cut(s) 131
PceI AGGCCT 1 cut(s) 355
PciSI GCTCTTC 1 cut(s) 659
PcsI WCGNNNNNNNCGW 1 cut(s) 144
PdiI GCCGGC 1 cut(s) 498
Pfl23II CGTACG 2 cut(s) 87, 555
PflFI GACNNNGTC 2 cut(s) 230, 299
PflMI CCANNNNNTGG 1 cut(s) 629
PkrI GCNGC 5 cut(s) 335, 408, 441, 520, 562
PleI GAGTC 1 cut(s) 586
PpsI GAGTC 1 cut(s) 586
PspEI GGTNACC 1 cut(s) 473
PspFI CCCAGC 1 cut(s) 721
PspLI CGTACG 2 cut(s) 87, 555
PspN4I GGNNCC 6 cut(s) 76, 284, 290, 405, 426, 453
PspPI GGNCC 2 cut(s) 74, 194
PstNI CAGNNNCTG 1 cut(s) 467
PsuI RGATCY 1 cut(s) 272
PsyI GACNNNGTC 2 cut(s) 230, 299
PvuII CAGCTG 1 cut(s) 265
RsaI GTAC 4 cut(s) 89, 557, 605, 644
RsaNI GTAC 4 cut(s) 88, 556, 604, 643
RseI CAYNNNNRTG 1 cut(s) 131
SapI GCTCTTC 1 cut(s) 659
SatI GCNGC 5 cut(s) 334, 407, 440, 519, 561
Sau3AI GATC 1 cut(s) 272
Sau96I GGNCC 2 cut(s) 74, 194
SchI GAGTC 1 cut(s) 586
ScrFI CCNGG 2 cut(s) 192, 574
SduI GDGCHC 1 cut(s) 252
SgrAI CRCCGGYG 1 cut(s) 538
SinI GGWCC 1 cut(s) 194
SmiMI CAYNNNNRTG 1 cut(s) 131
SmlI CTYRAG 2 cut(s) 50, 80
SmoI CTYRAG 2 cut(s) 50, 80
Sse9I AATT 1 cut(s) 768
SseBI AGGCCT 1 cut(s) 355
StuI AGGCCT 1 cut(s) 355
StyD4I CCNGG 2 cut(s) 190, 572
StyI CCWWGG 1 cut(s) 129
TaaI ACNGT 2 cut(s) 397, 608
TaiI ACGT 7 cut(s) 60, 155, 300, 312, 387, 557, 783
TaqI TCGA 3 cut(s) 105, 225, 303
TasI AATT 1 cut(s) 768
TauI GCSGC 4 cut(s) 336, 409, 442, 521
TscAI CASTG 1 cut(s) 402
TseI GCWGC 1 cut(s) 560
TspDTI ATGAA 1 cut(s) 333
TspRI CASTG 1 cut(s) 402
Tth111I GACNNNGTC 2 cut(s) 230, 299
Van91I CCANNNNNTGG 1 cut(s) 629
VpaK11BI GGWCC 1 cut(s) 194
XcmI CCANNNNNNNNNTGG 1 cut(s) 265
ZraI GACGTC 2 cut(s) 310, 385
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.