RchiOBHm_Chr1g0346771

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0002350
Physical Location & Seq
Forward (+)
39311473 .. 39312069
597 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 597 bp
ATGAACTCAATGGACTCGAACCAGGCGAAATCCAGCCTACTGTGTTCCCCAGAAGCCTCTCCGTCTCAGAGAACACTGAACCCCTACGTGACTGGCACGTCTGTTGTGGCTCTCAAATACAAAGATGGAGTTTTGATGGCTGCTGATATGGGAGGTTCCTATGGATCGACCCTCCGATACAAGAGCATCGAACGAATGAAGCCTATTGGAAAGCATTCTCTTCTCGGCGCAAGTGGAGAAATCAGTGATTTCCAGGAGCTGCTACGTTATCTTGACGAGCTCACCCAACAGGACAGCATGTGTGACGATGGAAACTCTTTGGGACCTAAAGAGATCCACAGCTACTTGATCCGTGTGATGTACAACAGGCGTAACAAGTTTGATCCGCTGTGGAACTCGCTTGTGCTTGGTGGAGTGAAGAACGGTAAGACATACCTCGGAATCGTTACTATGATAGGTTTGAACTTTGAGGACAATCATGTCGCTACCGGATTTGGAAACCACCTTGCAAGGCCTATTCTTCGTGATGAATGGCGCAAGGACTTGAGTTTCGAAGAAGGTGTTAGACTTCTGGAGAAATGCATGCGTGTATCATGA

Protein Analysis

198

Amino Acids

22.12

Weight (kDa)

7.71

Isoelectric Point (pI)

36.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proteasome PF00227 31 - 196 1e-30 Proteasome subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 97, 479
AciI CCGC 1 cut(s) 386
AclWI GGATC 4 cut(s) 172, 328, 343, 377
AfaI GTAC 1 cut(s) 362
AgsI TTSAA 1 cut(s) 463
AjiI CACGTC 1 cut(s) 99
AjnI CCWGG 2 cut(s) 21, 252
AluBI AGCT 3 cut(s) 259, 280, 342
AluI AGCT 3 cut(s) 259, 280, 342
Alw21I GWGCWC 1 cut(s) 282
Alw26I GTCTC 1 cut(s) 69
AlwI GGATC 4 cut(s) 172, 328, 343, 377
AlwNI CAGNNNCTG 1 cut(s) 259
AoxI GGCC 1 cut(s) 512
ApeKI GCWGC 2 cut(s) 140, 259
ArsI GACNNNNNNTTYG 2 cut(s) 533, 565
Asp700I GAANNNNTTC 1 cut(s) 214
AspLEI GCGC 2 cut(s) 230, 537
AspS9I GGNCC 1 cut(s) 323
AsuHPI GGTGA 1 cut(s) 274
AsuII TTCGAA 1 cut(s) 552
AvaII GGWCC 1 cut(s) 323
BanII GRGCYC 1 cut(s) 282
Bbv12I GWGCWC 1 cut(s) 282
BbvI GCAGC 2 cut(s) 127, 246
BccI CCATC 3 cut(s) 119, 130, 302
BciT130I CCWGG 2 cut(s) 23, 254
BcoDI GTCTC 1 cut(s) 69
BisI GCNGC 2 cut(s) 141, 260
BlsI GCNGC 2 cut(s) 142, 261
Bme1390I CCNGG 2 cut(s) 23, 254
Bme18I GGWCC 1 cut(s) 323
BmgBI CACGTC 1 cut(s) 99
BmgT120I GGNCC 1 cut(s) 323
BmiI GGNNCC 2 cut(s) 157, 324
BmrFI CCNGG 2 cut(s) 23, 254
BmsI GCATC 1 cut(s) 195
BpmI CTGGAG 1 cut(s) 593
Bpu14I TTCGAA 1 cut(s) 552
BpuEI CTTGAG 1 cut(s) 565
BsaAI YACGTR 1 cut(s) 88
BsaJI CCNNGG 1 cut(s) 436
BsaWI WCCGGW 1 cut(s) 488
Bse1I ACTGG 1 cut(s) 97
BseBI CCWGG 2 cut(s) 23, 254
BseDI CCNNGG 1 cut(s) 436
BseMII CTCAG 1 cut(s) 80
BseNI ACTGG 1 cut(s) 97
BseXI GCAGC 2 cut(s) 127, 246
BshFI GGCC 1 cut(s) 514
BsiHKAI GWGCWC 1 cut(s) 282
BsiSI CCGG 1 cut(s) 489
BslFI GGGAC 1 cut(s) 336
BsmAI GTCTC 1 cut(s) 69
BsmBI CGTCTC 1 cut(s) 69
BsmFI GGGAC 1 cut(s) 336
BsmI GAATGC 1 cut(s) 214
BsnI GGCC 1 cut(s) 514
Bsp119I TTCGAA 1 cut(s) 552
Bsp1286I GDGCHC 1 cut(s) 282
Bsp1407I TGTACA 1 cut(s) 360
Bsp143I GATC 4 cut(s) 164, 333, 348, 382
BspACI CCGC 1 cut(s) 386
BspANI GGCC 1 cut(s) 514
BspCNI CTCAG 1 cut(s) 79
BspHI TCATGA 1 cut(s) 593
BspLI GGNNCC 2 cut(s) 157, 324
BspPI GGATC 4 cut(s) 172, 328, 343, 377
BspT104I TTCGAA 1 cut(s) 552
BsrGI TGTACA 1 cut(s) 360
BsrI ACTGG 1 cut(s) 97
BssECI CCNNGG 1 cut(s) 436
BssMI GATC 4 cut(s) 164, 333, 348, 382
Bst2UI CCWGG 2 cut(s) 23, 254
Bst4CI ACNGT 2 cut(s) 42, 425
Bst6I CTCTTC 1 cut(s) 225
BstAUI TGTACA 1 cut(s) 360
BstBAI YACGTR 1 cut(s) 88
BstBI TTCGAA 1 cut(s) 552
BstC8I GCNNGC 1 cut(s) 584
BstDEI CTNAG 1 cut(s) 66
BstHHI GCGC 2 cut(s) 230, 537
BstKTI GATC 4 cut(s) 167, 336, 351, 385
BstMAI GTCTC 1 cut(s) 69
BstMBI GATC 4 cut(s) 164, 333, 348, 382
BstNI CCWGG 2 cut(s) 23, 254
BstNSI RCATGY 2 cut(s) 301, 586
BstSCI CCNGG 2 cut(s) 21, 252
BstV1I GCAGC 2 cut(s) 127, 246
BstX2I RGATCY 1 cut(s) 333
BstYI RGATCY 1 cut(s) 333
BsuRI GGCC 1 cut(s) 514
BtrI CACGTC 1 cut(s) 99
BtsIMutI CAGTG 2 cut(s) 74, 250
Cac8I GCNNGC 1 cut(s) 584
CaiI CAGNNNCTG 1 cut(s) 259
CciI TCATGA 1 cut(s) 593
CfoI GCGC 2 cut(s) 230, 537
Cfr13I GGNCC 1 cut(s) 323
Csp6I GTAC 1 cut(s) 361
CviAII CATG 4 cut(s) 298, 479, 583, 594
CviJI RGCY 9 cut(s) 36, 56, 110, 140, 202, 259, 280, 342, 514
CviKI_1 RGCY 9 cut(s) 36, 56, 110, 140, 202, 259, 280, 342, 514
CviQI GTAC 1 cut(s) 361
DdeI CTNAG 1 cut(s) 66
DpnI GATC 4 cut(s) 166, 335, 350, 384
DpnII GATC 4 cut(s) 164, 333, 348, 382
DrdI GACNNNNNNGTC 2 cut(s) 97, 479
DseDI GACNNNNNNGTC 2 cut(s) 97, 479
Eam1104I CTCTTC 1 cut(s) 225
EarI CTCTTC 1 cut(s) 225
Ecl136II GAGCTC 1 cut(s) 280
Eco147I AGGCCT 1 cut(s) 514
Eco24I GRGCYC 1 cut(s) 282
Eco47I GGWCC 1 cut(s) 323
Eco53kI GAGCTC 1 cut(s) 280
EcoICRI GAGCTC 1 cut(s) 280
EcoO109I RGGNCCY 1 cut(s) 323
EcoRII CCWGG 2 cut(s) 21, 252
EcoT22I ATGCAT 1 cut(s) 584
EcoT38I GRGCYC 1 cut(s) 282
Esp3I CGTCTC 1 cut(s) 69
FaeI CATG 4 cut(s) 301, 482, 586, 597
FaiI YATR 8 cut(s) 149, 162, 299, 433, 452, 480, 584, 595
FaqI GGGAC 1 cut(s) 336
FatI CATG 4 cut(s) 297, 478, 582, 593
Fnu4HI GCNGC 2 cut(s) 141, 260
FriOI GRGCYC 1 cut(s) 282
Fsp4HI GCNGC 2 cut(s) 141, 260
GlaI GCGC 2 cut(s) 229, 536
GluI GCNGC 2 cut(s) 141, 260
GsuI CTGGAG 1 cut(s) 593
HaeIII GGCC 1 cut(s) 514
HapII CCGG 1 cut(s) 489
HhaI GCGC 2 cut(s) 230, 537
Hin1II CATG 4 cut(s) 301, 482, 586, 597
Hin6I GCGC 2 cut(s) 228, 535
HinP1I GCGC 2 cut(s) 228, 535
HinfI GANTC 2 cut(s) 14, 441
HpaII CCGG 1 cut(s) 489
HphI GGTGA 1 cut(s) 274
Hpy188I TCNGA 3 cut(s) 69, 176, 440
Hpy188III TCNNGA 4 cut(s) 272, 524, 572, 594
HpyAV CCTTC 1 cut(s) 551
HpyCH4III ACNGT 2 cut(s) 42, 425
HpyCH4IV ACGT 3 cut(s) 87, 98, 265
HpyCH4V TGCA 2 cut(s) 509, 582
HpyF3I CTNAG 1 cut(s) 66
HpySE526I ACGT 3 cut(s) 87, 98, 265
Hsp92II CATG 4 cut(s) 301, 482, 586, 597
HspAI GCGC 2 cut(s) 228, 535
Kzo9I GATC 4 cut(s) 164, 333, 348, 382
LmnI GCTCC 1 cut(s) 256
Lsp1109I GCAGC 2 cut(s) 127, 246
LweI GCATC 1 cut(s) 195
MaeII ACGT 3 cut(s) 87, 98, 265
MaeIII GTNAC 4 cut(s) 88, 302, 371, 445
MalI GATC 4 cut(s) 166, 335, 350, 384
MboI GATC 4 cut(s) 164, 333, 348, 382
MboII GAAGA 4 cut(s) 212, 430, 512, 566
MflI RGATCY 1 cut(s) 333
MhlI GDGCHC 1 cut(s) 282
MlyI GAGTC 1 cut(s) 8
MnlI CCTC 5 cut(s) 67, 146, 182, 446, 463
Mph1103I ATGCAT 1 cut(s) 584
MroXI GAANNNNTTC 1 cut(s) 214
MspA1I CMGCKG 1 cut(s) 388
MspI CCGG 1 cut(s) 489
MspR9I CCNGG 2 cut(s) 23, 254
Mva1269I GAATGC 1 cut(s) 214
MvaI CCWGG 2 cut(s) 23, 254
NdeII GATC 4 cut(s) 164, 333, 348, 382
NlaIII CATG 4 cut(s) 301, 482, 586, 597
NlaIV GGNNCC 2 cut(s) 157, 324
NmeAIII GCCGAG 1 cut(s) 204
NmuCI GTSAC 2 cut(s) 88, 302
NsiI ATGCAT 1 cut(s) 584
NspI RCATGY 2 cut(s) 301, 586
NspV TTCGAA 1 cut(s) 552
PaeI GCATGC 1 cut(s) 586
PagI TCATGA 1 cut(s) 593
PceI AGGCCT 1 cut(s) 514
PcsI WCGNNNNNNNCGW 1 cut(s) 23
PctI GAATGC 1 cut(s) 214
PdmI GAANNNNTTC 1 cut(s) 214
PfeI GAWTC 1 cut(s) 441
PfoI TCCNGGA 1 cut(s) 252
PkrI GCNGC 2 cut(s) 142, 261
PleI GAGTC 1 cut(s) 8
PpsI GAGTC 1 cut(s) 8
Ppu21I YACGTR 1 cut(s) 88
PpuMI RGGWCCY 1 cut(s) 323
Psp124BI GAGCTC 1 cut(s) 282
Psp5II RGGWCCY 1 cut(s) 323
Psp6I CCWGG 2 cut(s) 21, 252
PspGI CCWGG 2 cut(s) 21, 252
PspN4I GGNNCC 2 cut(s) 157, 324
PspPI GGNCC 1 cut(s) 323
PspPPI RGGWCCY 1 cut(s) 323
PstNI CAGNNNCTG 1 cut(s) 259
PsuI RGATCY 1 cut(s) 333
RsaI GTAC 1 cut(s) 362
RsaNI GTAC 1 cut(s) 361
SacI GAGCTC 1 cut(s) 282
SatI GCNGC 2 cut(s) 141, 260
Sau3AI GATC 4 cut(s) 164, 333, 348, 382
Sau96I GGNCC 1 cut(s) 323
SchI GAGTC 1 cut(s) 8
ScrFI CCNGG 2 cut(s) 23, 254
SduI GDGCHC 1 cut(s) 282
SfaNI GCATC 1 cut(s) 195
SfuI TTCGAA 1 cut(s) 552
SinI GGWCC 1 cut(s) 323
SmlI CTYRAG 1 cut(s) 544
SmoI CTYRAG 1 cut(s) 544
SphI GCATGC 1 cut(s) 586
SseBI AGGCCT 1 cut(s) 514
SsiI CCGC 1 cut(s) 386
SstI GAGCTC 1 cut(s) 282
StuI AGGCCT 1 cut(s) 514
StyD4I CCNGG 2 cut(s) 21, 252
TaaI ACNGT 2 cut(s) 42, 425
TaiI ACGT 3 cut(s) 90, 101, 268
TaqI TCGA 4 cut(s) 17, 167, 189, 552
TatI WGTACW 1 cut(s) 360
TfiI GAWTC 1 cut(s) 441
TscAI CASTG 2 cut(s) 81, 250
TseFI GTSAC 2 cut(s) 88, 302
TseI GCWGC 2 cut(s) 140, 259
Tsp45I GTSAC 2 cut(s) 88, 302
TspDTI ATGAA 3 cut(s) 17, 212, 543
TspGWI ACGGA 2 cut(s) 51, 341
TspRI CASTG 2 cut(s) 81, 250
VpaK11BI GGWCC 1 cut(s) 323
XceI RCATGY 2 cut(s) 301, 586
XmnI GAANNNNTTC 1 cut(s) 214
Zsp2I ATGCAT 1 cut(s) 584
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.