RchiOBHm_Chr1g0368991

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0003719
Physical Location & Seq
Forward (+)
58942868 .. 58943777
910 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 471 bp
ATGGCTCTCAGTCTCTTTGGCACCGGCCGACGCAGCAACGTCTTCGACCCATTTTCCCTCGACATCTGGGACCCCTTCCAGGGCATAGGCTCTCTCGTCAACTCCTCCTCCACTGCCGCCGACACCTCCGCCTTCGCTCAGACCCGAATCGACTGGAAGGAGACCCCGGAGGCCCACATCTTCAAGGCGGACCTCCCCGGACTGAAGAAGGAAGAAGTGAAGGTTGAGCTGGAGGAAGGGAATGTCCTTCAGATCAGCGGAGAGAGGAGCAGGGAGCAGGAGGAGAAGAATGACAAGTGGCACAGGGTGGAGAGGAGCAGCGGCAAGTTCCTGAGGAGGTTCAGGCTGCCGGATAATGCAAAGGTGGAACATGTCAAGGCTTCTATGGAGAATGGGGTTCTCACTGTGACCGTGCCCAAAGCAGAGGAGCAGAAGCCCCAGGTCAAGAGCATCGACATTTCTGGAGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0008150 GO:0009266 GO:0009408 GO:0009628 GO:0009987 GO:0016043 GO:0022607 GO:0043933 GO:0044085 GO:0050896 GO:0051259 GO:0065003 GO:0071840
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

156

Amino Acids

17.48

Weight (kDa)

5.97

Isoelectric Point (pI)

47.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 51 - 154 2.3e-32 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 20
AciI CCGC 5 cut(s) 117, 129, 188, 258, 321
AcoI YGGCCR 1 cut(s) 25
AcuI CTGAAG 2 cut(s) 224, 233
AdeI CACNNNGTG 1 cut(s) 307
AfiI CCNNNNNNNGG 2 cut(s) 79, 80
AflIII ACRYGT 1 cut(s) 370
AgsI TTSAA 1 cut(s) 184
AjnI CCWGG 2 cut(s) 78, 438
AluBI AGCT 1 cut(s) 229
AluI AGCT 1 cut(s) 229
Alw26I GTCTC 2 cut(s) 17, 155
AoxI GGCC 2 cut(s) 25, 171
ApeKI GCWGC 3 cut(s) 33, 318, 346
AspS9I GGNCC 3 cut(s) 70, 172, 190
AsuC2I CCSGG 2 cut(s) 167, 198
AvaII GGWCC 2 cut(s) 70, 190
AxyI CCTNAGG 1 cut(s) 332
BaeGI GKGCMC 1 cut(s) 417
BanI GGYRCC 1 cut(s) 20
BbsI GAAGAC 1 cut(s) 34
BbvI GCAGC 3 cut(s) 45, 330, 333
BcgI CGANNNNNNTGC 2 cut(s) 25, 59
BciT130I CCWGG 2 cut(s) 80, 440
BcnI CCSGG 2 cut(s) 167, 198
BcoDI GTCTC 2 cut(s) 17, 155
BisI GCNGC 5 cut(s) 34, 117, 319, 322, 347
BlsI GCNGC 5 cut(s) 35, 118, 320, 323, 348
Bme1390I CCNGG 4 cut(s) 80, 167, 198, 440
Bme18I GGWCC 2 cut(s) 70, 190
BmgT120I GGNCC 3 cut(s) 70, 172, 190
BmiI GGNNCC 4 cut(s) 22, 71, 72, 466
BmrFI CCNGG 4 cut(s) 80, 167, 198, 440
BmsI GCATC 1 cut(s) 459
BpiI GAAGAC 1 cut(s) 34
BpmI CTGGAG 1 cut(s) 251
BpuMI CCSGG 2 cut(s) 167, 198
BsaI GGTCTC 1 cut(s) 155
BsaJI CCNNGG 4 cut(s) 79, 165, 196, 438
BsaXI ACNNNNNCTCC 2 cut(s) 92, 122
Bsc4I CCNNNNNNNGG 2 cut(s) 79, 80
Bse118I RCCGGY 1 cut(s) 23
Bse1I ACTGG 1 cut(s) 158
Bse21I CCTNAGG 1 cut(s) 332
BseBI CCWGG 2 cut(s) 80, 440
BseDI CCNNGG 4 cut(s) 79, 165, 196, 438
BseLI CCNNNNNNNGG 2 cut(s) 79, 80
BseMII CTCAG 3 cut(s) 22, 152, 323
BseNI ACTGG 1 cut(s) 158
BseRI GAGGAG 7 cut(s) 94, 97, 280, 296, 328, 349, 440
BseSI GKGCMC 1 cut(s) 417
BseX3I CGGCCG 1 cut(s) 25
BseXI GCAGC 3 cut(s) 45, 330, 333
Bsh1285I CGRYCG 1 cut(s) 28
BshFI GGCC 2 cut(s) 27, 173
BshNI GGYRCC 1 cut(s) 20
BsiEI CGRYCG 1 cut(s) 28
BsiSI CCGG 4 cut(s) 24, 167, 198, 350
BslFI GGGAC 1 cut(s) 83
BslI CCNNNNNNNGG 2 cut(s) 79, 80
BsmAI GTCTC 2 cut(s) 17, 155
BsmFI GGGAC 1 cut(s) 83
BsnI GGCC 2 cut(s) 27, 173
Bso31I GGTCTC 1 cut(s) 155
Bsp1286I GDGCHC 1 cut(s) 417
Bsp143I GATC 1 cut(s) 252
BspACI CCGC 5 cut(s) 117, 129, 188, 258, 321
BspANI GGCC 2 cut(s) 27, 173
BspCNI CTCAG 3 cut(s) 21, 151, 324
BspLI GGNNCC 4 cut(s) 22, 71, 72, 466
BspT107I GGYRCC 1 cut(s) 20
BspTNI GGTCTC 1 cut(s) 155
BsrFI RCCGGY 1 cut(s) 23
BsrI ACTGG 1 cut(s) 158
BssAI RCCGGY 1 cut(s) 23
BssECI CCNNGG 4 cut(s) 79, 165, 196, 438
BssMI GATC 1 cut(s) 252
Bst2UI CCWGG 2 cut(s) 80, 440
Bst4CI ACNGT 2 cut(s) 406, 412
BstDEI CTNAG 3 cut(s) 8, 138, 332
BstKTI GATC 1 cut(s) 255
BstMAI GTCTC 2 cut(s) 17, 155
BstMBI GATC 1 cut(s) 252
BstMCI CGRYCG 1 cut(s) 28
BstMWI GCNNNNNNNGC 1 cut(s) 33
BstNI CCWGG 2 cut(s) 80, 440
BstNSI RCATGY 1 cut(s) 374
BstSCI CCNGG 4 cut(s) 78, 165, 196, 438
BstSLI GKGCMC 1 cut(s) 417
BstV1I GCAGC 3 cut(s) 45, 330, 333
BstV2I GAAGAC 1 cut(s) 34
BstZI CGGCCG 1 cut(s) 25
Bsu36I CCTNAGG 1 cut(s) 332
BsuRI GGCC 2 cut(s) 27, 173
BtsI GCAGTG 1 cut(s) 111
BtsIMutI CAGTG 2 cut(s) 111, 402
Cfr10I RCCGGY 1 cut(s) 23
Cfr13I GGNCC 3 cut(s) 70, 172, 190
CseI GACGC 1 cut(s) 39
CviAII CATG 1 cut(s) 371
CviJI RGCY 9 cut(s) 5, 27, 90, 173, 229, 346, 380, 436, 467
CviKI_1 RGCY 9 cut(s) 5, 27, 90, 173, 229, 346, 380, 436, 467
DdeI CTNAG 3 cut(s) 8, 138, 332
DpnI GATC 1 cut(s) 254
DpnII GATC 1 cut(s) 252
DraIII CACNNNGTG 1 cut(s) 307
EaeI YGGCCR 1 cut(s) 25
EagI CGGCCG 1 cut(s) 25
EciI GGCGGA 2 cut(s) 118, 203
EclXI CGGCCG 1 cut(s) 25
Eco31I GGTCTC 1 cut(s) 155
Eco47I GGWCC 2 cut(s) 70, 190
Eco52I CGGCCG 1 cut(s) 25
Eco57I CTGAAG 2 cut(s) 224, 233
Eco81I CCTNAGG 1 cut(s) 332
EcoO109I RGGNCCY 1 cut(s) 70
EcoRII CCWGG 2 cut(s) 78, 438
FaeI CATG 1 cut(s) 374
FaiI YATR 3 cut(s) 86, 372, 386
FaqI GGGAC 1 cut(s) 83
FatI CATG 1 cut(s) 370
Fnu4HI GCNGC 5 cut(s) 34, 117, 319, 322, 347
Fsp4HI GCNGC 5 cut(s) 34, 117, 319, 322, 347
GluI GCNGC 5 cut(s) 34, 117, 319, 322, 347
GsuI CTGGAG 1 cut(s) 251
HaeIII GGCC 2 cut(s) 27, 173
HapII CCGG 4 cut(s) 24, 167, 198, 350
HgaI GACGC 1 cut(s) 39
Hin1II CATG 1 cut(s) 374
HincII GTYRAC 1 cut(s) 100
HindII GTYRAC 1 cut(s) 100
HinfI GANTC 1 cut(s) 147
HpaII CCGG 4 cut(s) 24, 167, 198, 350
Hpy166II GTNNAC 1 cut(s) 100
Hpy188I TCNGA 2 cut(s) 141, 252
Hpy188III TCNNGA 3 cut(s) 331, 445, 462
Hpy8I GTNNAC 1 cut(s) 100
Hpy99I CGWCG 1 cut(s) 33
HpyAV CCTTC 7 cut(s) 85, 142, 151, 202, 214, 230, 257
HpyCH4III ACNGT 2 cut(s) 406, 412
HpyCH4IV ACGT 1 cut(s) 39
HpyCH4V TGCA 1 cut(s) 359
HpyF10VI GCNNNNNNNGC 1 cut(s) 33
HpyF3I CTNAG 3 cut(s) 8, 138, 332
HpySE526I ACGT 1 cut(s) 39
Hsp92II CATG 1 cut(s) 374
KflI GGGWCCC 1 cut(s) 70
Kzo9I GATC 1 cut(s) 252
LmnI GCTCC 5 cut(s) 267, 274, 315, 427, 464
Lsp1109I GCAGC 3 cut(s) 45, 330, 333
LweI GCATC 1 cut(s) 459
MaeII ACGT 1 cut(s) 39
MaeIII GTNAC 1 cut(s) 406
MalI GATC 1 cut(s) 254
MboI GATC 1 cut(s) 252
MboII GAAGA 5 cut(s) 34, 172, 217, 224, 298
MhlI GDGCHC 1 cut(s) 417
MspA1I CMGCKG 2 cut(s) 258, 321
MspI CCGG 4 cut(s) 24, 167, 198, 350
MspR9I CCNGG 4 cut(s) 80, 167, 198, 440
MvaI CCWGG 2 cut(s) 80, 440
MwoI GCNNNNNNNGC 1 cut(s) 33
NciI CCSGG 2 cut(s) 167, 198
NdeII GATC 1 cut(s) 252
NlaIII CATG 1 cut(s) 374
NlaIV GGNNCC 4 cut(s) 22, 71, 72, 466
NmuCI GTSAC 1 cut(s) 406
NspI RCATGY 1 cut(s) 374
PciI ACATGT 1 cut(s) 370
PfeI GAWTC 1 cut(s) 147
PkrI GCNGC 5 cut(s) 35, 118, 320, 323, 348
PpuMI RGGWCCY 1 cut(s) 70
PscI ACATGT 1 cut(s) 370
Psp5II RGGWCCY 1 cut(s) 70
Psp6I CCWGG 2 cut(s) 78, 438
PspGI CCWGG 2 cut(s) 78, 438
PspN4I GGNNCC 4 cut(s) 22, 71, 72, 466
PspPI GGNCC 3 cut(s) 70, 172, 190
PspPPI RGGWCCY 1 cut(s) 70
SatI GCNGC 5 cut(s) 34, 117, 319, 322, 347
Sau3AI GATC 1 cut(s) 252
Sau96I GGNCC 3 cut(s) 70, 172, 190
ScrFI CCNGG 4 cut(s) 80, 167, 198, 440
SduI GDGCHC 1 cut(s) 417
SetI ASST 8 cut(s) 42, 128, 195, 225, 231, 341, 366, 444
SfaNI GCATC 1 cut(s) 459
SinI GGWCC 2 cut(s) 70, 190
SsiI CCGC 5 cut(s) 117, 129, 188, 258, 321
StyD4I CCNGG 4 cut(s) 78, 165, 196, 438
TaaI ACNGT 2 cut(s) 406, 412
TaiI ACGT 1 cut(s) 42
TaqI TCGA 4 cut(s) 45, 60, 150, 453
TauI GCSGC 2 cut(s) 119, 324
TfiI GAWTC 1 cut(s) 147
TscAI CASTG 2 cut(s) 118, 409
TseFI GTSAC 1 cut(s) 406
TseI GCWGC 3 cut(s) 33, 318, 346
Tsp45I GTSAC 1 cut(s) 406
TspRI CASTG 2 cut(s) 118, 409
VpaK11BI GGWCC 2 cut(s) 70, 190
XceI RCATGY 1 cut(s) 374
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.