RchiOBHm_Chr1g0318941

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0004242
Physical Location & Seq
Forward (+)
6569018 .. 6573801
4784 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ54919

Sequence Viewer

Length: 4326 bp
ATGCCTTTTCGTCGAGCACTTGAATCTGCTTCTGTAAGCATCCTCCTTAACAAGTTGGCCACTCAAGATGTCATAGATTTCTTCCTCAAGTGGAAAATCGATGATTCCCTGCGAGAAAAGCTCAAGAGAAACCTGCTTGTCATTCATGCGGTTCTCAATCATGCTGAGGAGAAGCAAGTCCGGGACTCAAATGTCAAAGCTTGGCTCGAGGACGTCAGAGTTGCAGCTTATGATGCCGAGGACATATTGGGTGGCATCGCCAATGATGCTCTCGAATCACAAAACAGCAAACATGAGGTATGGAATTCTAAGATCAAAGAAGGGATAGACTTTAAGATGAAAGACATTGCTAATACTCTTAATCCCTTTAGGGAACGTGTAGAGTCGAAAATGATAAAGATTATAGATGTATTGGAAGAGATTGCAAAGCAGAAAGATGTTCTTCGTTTGAGAGAAGATTCTGGCGGTATAGAATCAGGTTTTGACGGATTGCCCACGACTCCAATGTTGGGTGATAAATCTCATGTTTATGGTAGAGAATCTGATAAGAAGGAGATAATTGCTTTGCTGAAATCGGGTAAAAATGATCGTGAGGTTTCTATAATTCCAATTGTAGGCATGGGAGGCATTGGGAAGACTACTCTTGCTCAGCTTGTGTATAATGATGCCAAAGTGAGTAGCTATTTTAATTTGAGAGCCTGGGCTTGTGTCTCTGATGTATTTGATGTGCAAAGGATAACAAAAACACTAGTTGATTCCGCCACAAAATCAAGTTGTAGCACAAACAATTTGGAGTTACTTCAAGAACAGCTGAAAATACGGTTGAAGAACAAGAAATTTCTTTTTGTTCTGGATGATGTCTGGAACGACAAGTATGAAAGCTGGGATAAGTTAAGAGTCCCCTTCACAGTTGGTGCACCAGGGAGTAAAATCATAATCACGACACGAAACAAAACAGTTGCATCACTTATGGGGACTGTTCCTACTTACTGTTTGGAGGGGCTTTCGGACAGTGATTGTTGGTTATTATTCGAACAAATTGTGTTTCAGAACAGGAATTTAGATGCCTATCCAAAGTTGAAAGTGATTGGCAAGAAAATAGCAGATAAGTGTAAAGGGTTGCCTTTAGCTGCAAAGGCACTAGGAGGTCTCCTGCGTGCTGAACCGGAACTAGATGAGAATTACTGGAATGATATCTTGAATAGCAAACTTTGGAAACTGGCAGACAGCACTATTCTTCCAGCTCTAAGATTAAGCTACCATCACCTCCCTGGGCATTTGAAGCGCTGCTTTGCTTATTGCTCGATGTTTCCTAAGGACTATGAATATGAGAGAGAGATGTTAATCTTGTTATGGATGGCAGAGGGTTTTGTGCCAGAGCCAGAAGGAAATCAACGGATCGAAGATGTAGCTGGAAGATATGTTTCAGATTTTTTATCAAGGTCCTTCTTCCAACACACCAGTGACAAGTCACGACTGGTAATGCATGACCTCATACATGATCTTGCACAATCTGTTTCTGGCAAAACATTTGTTAGGTTGGAGGAAAATTCAGAGAACCATAAAAAGTCTACAAAGGCTCGGCACTTGTCATATACCCGTGGATCTAAAGATGTATTTCAAAAATTTGAGGCATTCAGTGAGGTGGATTGTTTACGAACCTTTCTACCACTAGACCCGTTGCAAGGATTCAATGTGAGTGGCTTATCTGACAAGGTTCCTTGTGATATGATGCCAAAATTAAGACTCCTGCGGGTGCTATCCTTTAGTGGTTATCTCATAGAAAAGTTGCCAGATTCAATTGGCAACTTGAAGCATCTGCGATACCTCAATCTTTCTTATTGTCAAATAGAAGAGCTACCTGAGCCAACAAGCAGTCTATACAATCTGCAGACATTAATTTTATTCAGATGTCGATCGCTTACCATGCTGCCTGCAGACATAGGAAACCTAAGGAATTTACGGCATCTCAATATTCAGGGAACTAGGCTGAAAAGCATGCCTTTGGGAATGGGAAGATTGGTTAACCTTCAAATACTGTCAGATTTTATTGTGGGGAAAGCCATGGGAAAAGGGATTGCAGAATTGAAGGACTTGTCACATCTTCGAGGCTCAGTTTCTATTTTGTGTTTGCATGATGTCAGCAGTGTTAGGGATGCAATTGAGGCCAGATTAGAGGTTAAGAAGCACCTTGATGATTTAGTTTTGGAATGGAGCAGCAGTACTGATGGTTCGCGAAATGAAGAGATCGAGACGGAGGTACTTGATGCACTGCAGCCTCATGAAAACCTGAAGAAACTCACCATTAAGTATTATGGAGGTACAGAATTTCCATATTGGATGGGGAATCCTTTGTTCACTAACATGGCGTACTTGCATCTCTATGGTTGCATAAAATGCACATCTTTACCACCCCTTGGCCAGCTACCTTCTCTTAAAGATCTCATAATTGAAGCAATGCATGGAATACAGCGTGTAGGAATGGATTTTTTTGGGGATGATGATGGTTCCACAATTTTTTTCCCCTCTTTAGAGACTTTGAAATTTGAGGACATGAAAGAATGGGAGAAATGGTCATCTTCAGAAGATGAAAGGCTTAAAGGATTTCCCAGCCTTCGGGAGCTTTCCATATTTAGATGTCCCAAGTTAACAAAGTTTACACGTGGATTTGCTTCTCTAGAAAAGCTGCTCATCAAGAACTGCGGAGCAATAACTACCTTCTCACAGAATCCTGCACTTGGGAATTTGGAACCTGTAGAATTCCCTAGCCTGAATTTGGAACCTGTAGAATTCCCTAGCCTGCAACTGCTTGTTCTTGTTGGTTGCAGTGAGCTTGAAAACCTTCCTTTTTCCCTCCCTTTGTTGAAAATGCTTGACATAGAGGGTTGTAAAAAATTGGCTGCACTTCCGAGGATGGTGGAACTTAGTACATTGTACCTGATCGATTCAAATGCTGAATTACTTGGATCCAAGATGGAATTTAGCTCGCTGACCTCTTTGCACCTAACTGATATTCCAGATGTGAAGCATCTACCTGAAGGCTTTATCCAACAGTCTGCAAAACTTGAAAAATTGAGTCTTACAAGCTTTCCTGACCTTGAATATTTGGCAGATGACCGAGTTGGACTAGCACACCTTGTATCCCTTCAACGCTTGACAATATCCCATTGTCCTAAGTTTGTTGCTTTGCCTGATGAGCTCCCACCTGTACTTAAATATTTGGATTTGAAACATTGTCCTAGCCTGATGAAGCTGCCTGGTAAACTTCACAACCTTGAGTGTCTTGCAGAGCTGCAGATTGAATGGTGTTCAAAGATTGAAGCATTTCCAGATGTGGGGTTGCCTGACAAACTGAAGCGTCTTGTAATCCGCGAATGTGGTGCTCTGAAGACCCTTCCTCAGGAGCTTCTCTGCAATAACAATTTTCTCGAGTATTTAGAAGTTCATAAATGTCCTTCTCTTGTGTCAGTTCTTGAAGAAGGAAACTTGCCATCAACTCTAACACATATGAAATTTTACTATTGCAAAATTCTGGGATCACTGCCTGAGGGTTTGATGCACAAAGACAATATGACTCTAAAGTACTTGGAGATAGACAACTGTTCCTCTCTCATGTCCTTTCCAAATGGTGAGTTACCAACAAGCCTTGAGCGTCTTGAGATTAGTGATTGCTCTAATTTCCAGACATTGCCATTGAGCCTTCTCAACCTCACGAATCTTGAGACCTTGGAAGTAAAGAGCTGCCCCCTTCTTCAGGACTTTCCAGAGGGTGGATTGCCACCCAACATAAAGTTTGTCACAATTTCCGAATGTAAGAATCTCAAGTCTCTACCTAAATTCTTTCATAAACTCAAATGTCTTCAGAACTTGGAGATTTCTGGCTGTCCTAGTCTCCTGTCCTTGCCAAAGCAGGGTCTGCCCAGCAGCTTAAGACTACTTACAGTTACTGATTGTGAAAAGCTTAATCCCATTCATCAGTGGAGACTTCACAAACTACCCTCTCTAGATGTTTTCACCATTGGTGGATTTCCTGGCCTGAAGTCCTTTTCAAAAGATTATCCTCTTCCTCACACTTTAACCTCTTTAAGCATTCAAAGATTCCCAGATCTTGAATCCATATCGGAGGTGCTTGAGAATCTCACCTCACTTGAAAACCTGAGTATCAGGGAGTGTGATAAGCTCCAATCTTTGCCAGAGACGGGGCTACCAGCGACAATTTCTACCTTGACAATTCGGAATTGTTTGCTTCTAGAACCTCGATGTGAAAAGGACAAAGGAGAAGATTGGTCCAAGATCAAAAACATCCCCTTCGTGAGTTCACGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

1441

Amino Acids

162.82

Weight (kDa)

6.18

Isoelectric Point (pI)

46.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 12 - 98 7.2e-15 Rx N-terminal domain
NB-ARC PF00931 181 - 352 2.6e-35 NB-ARC domain
WHD_DRP PF23559 436 - 503 1.1e-24 Disease resistance protein Winged helix domain
LRR_14 PF23598 578 - 850 5.1e-31 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 693 - 820 2.7e-40 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 191
AatII GACGTC 1 cut(s) 216
Acc36I ACCTGC 1 cut(s) 141
AccB7I CCANNNNNTGG 2 cut(s) 2417, 3770
AccI GTMKAC 1 cut(s) 1571
AccII CGCG 2 cut(s) 2236, 3372
AciI CCGC 6 cut(s) 149, 465, 759, 1753, 2703, 3370
AclWI GGATC 5 cut(s) 1406, 1612, 2961, 2974, 3544
AcoI YGGCCR 2 cut(s) 57, 2419
AcuI CTGAAG 8 cut(s) 2312, 2565, 3057, 3374, 3407, 3737, 3845, 4058
AcvI CACGTG 1 cut(s) 2663
AcyI GRCGYC 1 cut(s) 213
AfaI GTAC 8 cut(s) 2224, 2262, 2323, 2372, 2929, 2936, 3210, 3584
AfeI AGCGCT 1 cut(s) 1286
AflII CTTAAG 1 cut(s) 3928
AflIII ACRYGT 2 cut(s) 376, 2660
AhlI ACTAGT 1 cut(s) 748
AjnI CCWGG 5 cut(s) 698, 919, 1270, 3256, 4030
AleI CACNNNNGTG 1 cut(s) 1461
Alw21I GWGCWC 4 cut(s) 19, 919, 3201, 3385
Alw26I GTCTC 9 cut(s) 715, 1154, 2246, 2528, 3716, 3831, 3896, 3976, 4190
Alw44I GTGCAC 1 cut(s) 915
AlwI GGATC 5 cut(s) 1406, 1612, 2961, 2974, 3544
AlwNI CAGNNNCTG 2 cut(s) 3916, 3947
Ama87I CYCGRG 2 cut(s) 206, 3428
Aor51HI AGCGCT 1 cut(s) 1286
AoxI GGCC 4 cut(s) 57, 2166, 2419, 4033
ApaLI GTGCAC 1 cut(s) 915
AseI ATTAAT 1 cut(s) 1898
Asp700I GAANNNNTTC 2 cut(s) 2841, 3324
AspLEI GCGC 1 cut(s) 1287
AspS9I GGNCC 2 cut(s) 1443, 4287
AsuC2I CCSGG 1 cut(s) 182
AsuHPI GGTGA 6 cut(s) 524, 1256, 2293, 3641, 4006, 4132
AsuII TTCGAA 1 cut(s) 1032
AvaI CYCGRG 2 cut(s) 206, 3428
AvaII GGWCC 2 cut(s) 1443, 4287
AxyI CCTNAGG 4 cut(s) 1314, 1952, 3399, 3546
BaeGI GKGCMC 1 cut(s) 919
BaeI ACNNNNGTAYC 2 cut(s) 4144, 4177
BalI TGGCCA 2 cut(s) 59, 2421
BamHI GGATCC 1 cut(s) 2966
BanII GRGCYC 1 cut(s) 3201
BbrPI CACGTG 1 cut(s) 2663
BbsI GAAGAC 3 cut(s) 641, 3395, 3851
Bbv12I GWGCWC 4 cut(s) 19, 919, 3201, 3385
BbvCI CCTCAGC 1 cut(s) 165
BccI CCATC 8 cut(s) 1269, 1351, 2222, 2335, 2498, 2908, 2968, 3499
BceAI ACGGC 1 cut(s) 1979
BcgI CGANNNNNNTGC 4 cut(s) 2933, 2967, 3362, 3396
BciT130I CCWGG 5 cut(s) 700, 921, 1272, 3258, 4032
BciVI GTATCC 1 cut(s) 3151
BcnI CCSGG 1 cut(s) 182
BcoDI GTCTC 9 cut(s) 715, 1154, 2246, 2528, 3716, 3831, 3896, 3976, 4190
BcuI ACTAGT 1 cut(s) 748
BfmI CTRYAG 6 cut(s) 1889, 1935, 2273, 2754, 2784, 3293
BfoI RGCGCY 1 cut(s) 1288
BfrI CTTAAG 1 cut(s) 3928
BfuAI ACCTGC 1 cut(s) 141
BfuI GTATCC 1 cut(s) 3151
BglII AGATCT 2 cut(s) 2440, 4105
BlpI GCTNAGC 1 cut(s) 648
BmcAI AGTACT 2 cut(s) 2224, 3584
Bme1390I CCNGG 6 cut(s) 182, 700, 921, 1272, 3258, 4032
Bme18I GGWCC 2 cut(s) 1443, 4287
BmeT110I CYCGRG 2 cut(s) 206, 3428
BmgT120I GGNCC 2 cut(s) 1443, 4287
BmiI GGNNCC 5 cut(s) 1719, 2509, 2751, 2781, 2968
BmrFI CCNGG 6 cut(s) 182, 700, 921, 1272, 3258, 4032
BpiI GAAGAC 3 cut(s) 641, 3395, 3851
BplI GAGNNNNNCTC 2 cut(s) 105, 137
Bpu10I CCTNAGC 2 cut(s) 165, 1863
Bpu1102I GCTNAGC 1 cut(s) 648
Bpu14I TTCGAA 1 cut(s) 1032
BpuEI CTTGAG 9 cut(s) 48, 71, 107, 3296, 3668, 3677, 3740, 3806, 4151
BpuMI CCSGG 1 cut(s) 182
Bsa29I ATCGAT 2 cut(s) 99, 2943
BsaAI YACGTR 1 cut(s) 2663
BsaBI GATNNNNATC 3 cut(s) 1068, 1343, 1915
BsaHI GRCGYC 1 cut(s) 213
BsaI GGTCTC 2 cut(s) 1154, 3716
BsaWI WCCGGW 1 cut(s) 1165
BsaXI ACNNNNNCTCC 4 cut(s) 2206, 2236, 2558, 2588
Bse1I ACTGG 4 cut(s) 1190, 1224, 1461, 1482
Bse21I CCTNAGG 4 cut(s) 1314, 1952, 3399, 3546
Bse3DI GCAATG 3 cut(s) 345, 2463, 3686
Bse8I GATNNNNATC 3 cut(s) 1068, 1343, 1915
BseBI CCWGG 5 cut(s) 700, 921, 1272, 3258, 4032
BseCI ATCGAT 2 cut(s) 99, 2943
BseGI GGATG 8 cut(s) 39, 859, 1362, 2161, 2346, 2503, 2919, 4302
BseJI GATNNNNATC 3 cut(s) 1068, 1343, 1915
BseMI GCAATG 3 cut(s) 345, 2463, 3686
BseMII CTCAG 7 cut(s) 156, 662, 1854, 2127, 3413, 3537, 4148
BseNI ACTGG 4 cut(s) 1190, 1224, 1461, 1482
BseRI GAGGAG 1 cut(s) 182
BseSI GKGCMC 1 cut(s) 919
BseYI CCCAGC 3 cut(s) 882, 2609, 3920
BsgI GTGCAG 2 cut(s) 2718, 2886
Bsh1236I CGCG 2 cut(s) 2236, 3372
Bsh1285I CGRYCG 1 cut(s) 1919
BshFI GGCC 4 cut(s) 59, 2168, 2421, 4035
BshVI ATCGAT 2 cut(s) 99, 2943
BsiEI CGRYCG 1 cut(s) 1919
BsiHKAI GWGCWC 4 cut(s) 19, 919, 3201, 3385
BsiHKCI CYCGRG 2 cut(s) 206, 3428
BsiSI CCGG 2 cut(s) 181, 1166
BslFI GGGAC 4 cut(s) 197, 884, 988, 2625
BsmAI GTCTC 9 cut(s) 715, 1154, 2246, 2528, 3716, 3831, 3896, 3976, 4190
BsmBI CGTCTC 2 cut(s) 2246, 4190
BsmFI GGGAC 4 cut(s) 197, 884, 988, 2625
BsmI GAATGC 2 cut(s) 1634, 4089
BsnI GGCC 4 cut(s) 59, 2168, 2421, 4035
Bso31I GGTCTC 2 cut(s) 1154, 3716
BsoBI CYCGRG 2 cut(s) 206, 3428
Bsp119I TTCGAA 1 cut(s) 1032
Bsp1286I GDGCHC 4 cut(s) 19, 919, 3201, 3385
Bsp1720I GCTNAGC 1 cut(s) 648
Bsp19I CCATGG 1 cut(s) 2064
Bsp68I TCGCGA 1 cut(s) 2236
BspACI CCGC 6 cut(s) 149, 465, 759, 1753, 2703, 3370
BspANI GGCC 4 cut(s) 59, 2168, 2421, 4035
BspCNI CTCAG 7 cut(s) 157, 661, 1855, 2126, 3412, 3538, 4149
BspDI ATCGAT 2 cut(s) 99, 2943
BspFNI CGCG 2 cut(s) 2236, 3372
BspHI TCATGA 1 cut(s) 2281
BspLI GGNNCC 5 cut(s) 1719, 2509, 2751, 2781, 2968
BspMAI CTGCAG 4 cut(s) 1893, 1939, 2277, 3297
BspMI ACCTGC 1 cut(s) 141
BspPI GGATC 5 cut(s) 1406, 1612, 2961, 2974, 3544
BspQI GCTCTTC 1 cut(s) 1848
BspT104I TTCGAA 1 cut(s) 1032
BspTI CTTAAG 1 cut(s) 3928
BspTNI GGTCTC 2 cut(s) 1154, 3716
BsrDI GCAATG 3 cut(s) 345, 2463, 3686
BsrI ACTGG 4 cut(s) 1190, 1224, 1461, 1482
BssNI GRCGYC 1 cut(s) 213
BssT1I CCWWGG 3 cut(s) 2064, 2416, 3726
Bst2UI CCWGG 5 cut(s) 700, 921, 1272, 3258, 4032
Bst6I CTCTTC 4 cut(s) 411, 1848, 2238, 4068
BstACI GRCGYC 1 cut(s) 213
BstAFI CTTAAG 1 cut(s) 3928
BstAPI GCANNNNNTGC 2 cut(s) 2397, 3916
BstBAI YACGTR 1 cut(s) 2663
BstBI TTCGAA 1 cut(s) 1032
BstC8I GCNNGC 6 cut(s) 1158, 1935, 2000, 2423, 2801, 2987
BstDSI CCRYGG 2 cut(s) 1600, 2064
BstENI CCTNNNNNAGG 2 cut(s) 3398, 3752
BstF5I GGATG 8 cut(s) 39, 859, 1362, 2161, 2346, 2503, 2919, 4302
BstFNI CGCG 2 cut(s) 2236, 3372
BstH2I RGCGCY 1 cut(s) 1288
BstHHI GCGC 1 cut(s) 1287
BstMAI GTCTC 9 cut(s) 715, 1154, 2246, 2528, 3716, 3831, 3896, 3976, 4190
BstMCI CGRYCG 1 cut(s) 1919
BstNI CCWGG 5 cut(s) 700, 921, 1272, 3258, 4032
BstNSI RCATGY 1 cut(s) 2002
BstSCI CCNGG 6 cut(s) 180, 698, 919, 1270, 3256, 4030
BstSFI CTRYAG 6 cut(s) 1889, 1935, 2273, 2754, 2784, 3293
BstSLI GKGCMC 1 cut(s) 919
BstUI CGCG 2 cut(s) 2236, 3372
BstV2I GAAGAC 3 cut(s) 641, 3395, 3851
BstX2I RGATCY 4 cut(s) 1604, 2440, 2966, 4105
BstYI RGATCY 4 cut(s) 1604, 2440, 2966, 4105
Bsu15I ATCGAT 2 cut(s) 99, 2943
Bsu36I CCTNAGG 4 cut(s) 1314, 1952, 3399, 3546
BsuI GTATCC 1 cut(s) 3151
BsuRI GGCC 4 cut(s) 59, 2168, 2421, 4035
BsuTUI ATCGAT 2 cut(s) 99, 2943
BtgI CCRYGG 2 cut(s) 1600, 2064
BtgZI GCGATG 1 cut(s) 241
BtsCI GGATG 8 cut(s) 39, 859, 1362, 2161, 2346, 2503, 2919, 4302
BtsI GCAGTG 4 cut(s) 2152, 2270, 2833, 3539
BtsIMutI CAGTG 8 cut(s) 1018, 1468, 1645, 2152, 2270, 2833, 3539, 3983
BtuMI TCGCGA 1 cut(s) 2236
BveI ACCTGC 1 cut(s) 141
Cac8I GCNNGC 6 cut(s) 1158, 1935, 2000, 2423, 2801, 2987
CaiI CAGNNNCTG 2 cut(s) 3916, 3947
CciI TCATGA 1 cut(s) 2281
CfoI GCGC 1 cut(s) 1287
Cfr13I GGNCC 2 cut(s) 1443, 4287
ClaI ATCGAT 2 cut(s) 99, 2943
CseI GACGC 2 cut(s) 3347, 3641
Csp6I GTAC 8 cut(s) 2223, 2261, 2322, 2371, 2928, 2935, 3209, 3583
CspCI CAANNNNNGTGG 2 cut(s) 1681, 1716
CviQI GTAC 8 cut(s) 2223, 2261, 2322, 2371, 2928, 2935, 3209, 3583
DrdI GACNNNNNNGTC 1 cut(s) 191
DseDI GACNNNNNNGTC 1 cut(s) 191
EaeI YGGCCR 2 cut(s) 57, 2419
Eam1104I CTCTTC 4 cut(s) 411, 1848, 2238, 4068
EarI CTCTTC 4 cut(s) 411, 1848, 2238, 4068
EciI GGCGGA 1 cut(s) 748
Ecl136II GAGCTC 1 cut(s) 3199
Eco130I CCWWGG 3 cut(s) 2064, 2416, 3726
Eco24I GRGCYC 1 cut(s) 3201
Eco31I GGTCTC 2 cut(s) 1154, 3716
Eco32I GATATC 1 cut(s) 1195
Eco47I GGWCC 2 cut(s) 1443, 4287
Eco47III AGCGCT 1 cut(s) 1286
Eco53kI GAGCTC 1 cut(s) 3199
Eco57I CTGAAG 8 cut(s) 2312, 2565, 3057, 3374, 3407, 3737, 3845, 4058
Eco72I CACGTG 1 cut(s) 2663
Eco81I CCTNAGG 4 cut(s) 1314, 1952, 3399, 3546
Eco88I CYCGRG 2 cut(s) 206, 3428
EcoICRI GAGCTC 1 cut(s) 3199
EcoNI CCTNNNNNAGG 2 cut(s) 3398, 3752
EcoO109I RGGNCCY 1 cut(s) 1443
EcoRI GAATTC 3 cut(s) 304, 2759, 2789
EcoRII CCWGG 5 cut(s) 698, 919, 1270, 3256, 4030
EcoRV GATATC 1 cut(s) 1195
EcoT14I CCWWGG 3 cut(s) 2064, 2416, 3726
EcoT22I ATGCAT 2 cut(s) 1488, 2463
EcoT38I GRGCYC 1 cut(s) 3201
ErhI CCWWGG 3 cut(s) 2064, 2416, 3726
Esp3I CGTCTC 2 cut(s) 2246, 4190
FalI AAGNNNNNCTT 6 cut(s) 426, 458, 1274, 1306, 1987, 2019
FaqI GGGAC 4 cut(s) 197, 884, 988, 2625
FauI CCCGC 1 cut(s) 1746
FauNDI CATATG 1 cut(s) 3507
FblI GTMKAC 1 cut(s) 1571
FokI GGATG 8 cut(s) 26, 866, 1369, 2168, 2353, 2510, 2926, 4289
FriOI GRGCYC 1 cut(s) 3201
GlaI GCGC 1 cut(s) 1286
GsaI CCCAGC 3 cut(s) 886, 2613, 3924
HaeII RGCGCY 1 cut(s) 1288
HaeIII GGCC 4 cut(s) 59, 2168, 2421, 4035
HapII CCGG 2 cut(s) 181, 1166
HgaI GACGC 2 cut(s) 3347, 3641
HhaI GCGC 1 cut(s) 1287
Hin1I GRCGYC 1 cut(s) 213
Hin6I GCGC 1 cut(s) 1285
HinP1I GCGC 1 cut(s) 1285
HincII GTYRAC 2 cut(s) 2026, 2649
HindII GTYRAC 2 cut(s) 2026, 2649
HindIII AAGCTT 3 cut(s) 198, 3085, 3959
HpaI GTTAAC 2 cut(s) 2026, 2649
HpaII CCGG 2 cut(s) 181, 1166
HphI GGTGA 6 cut(s) 524, 1256, 2293, 3641, 4006, 4132
Hpy166II GTNNAC 9 cut(s) 917, 1572, 1655, 2026, 2358, 2649, 2658, 3263, 4319
Hpy8I GTNNAC 9 cut(s) 917, 1572, 1655, 2026, 2358, 2649, 2658, 3263, 4319
Hpy99I CGWCG 1 cut(s) 15
HpyCH4IV ACGT 3 cut(s) 213, 376, 2662
HpySE526I ACGT 3 cut(s) 213, 376, 2662
Hsp92I GRCGYC 1 cut(s) 213
HspAI GCGC 1 cut(s) 1285
KspAI GTTAAC 2 cut(s) 2026, 2649
LguI GCTCTTC 1 cut(s) 1848
LmnI GCTCC 6 cut(s) 2214, 2620, 2705, 3204, 3403, 4185
MaeII ACGT 3 cut(s) 213, 376, 2662
MaeIII GTNAC 7 cut(s) 795, 1463, 1470, 2097, 3633, 3796, 3943
MfeI CAATTG 3 cut(s) 609, 1800, 2160
MflI RGATCY 4 cut(s) 1604, 2440, 2966, 4105
MhlI GDGCHC 4 cut(s) 19, 919, 3201, 3385
MlsI TGGCCA 2 cut(s) 59, 2421
MluNI TGGCCA 2 cut(s) 59, 2421
MlyI GAGTC 7 cut(s) 179, 392, 493, 906, 1740, 3085, 3568
MmeI TCCRAC 4 cut(s) 1477, 1521, 3073, 3103
Mox20I TGGCCA 2 cut(s) 59, 2421
Mph1103I ATGCAT 2 cut(s) 1488, 2463
MroXI GAANNNNTTC 2 cut(s) 2841, 3324
MscI TGGCCA 2 cut(s) 59, 2421
MslI CAYNNNNRTG 5 cut(s) 528, 1461, 2193, 2363, 2382
Msp20I TGGCCA 2 cut(s) 59, 2421
MspA1I CMGCKG 1 cut(s) 811
MspCI CTTAAG 1 cut(s) 3928
MspI CCGG 2 cut(s) 181, 1166
MspR9I CCNGG 6 cut(s) 182, 700, 921, 1272, 3258, 4032
MunI CAATTG 3 cut(s) 609, 1800, 2160
Mva1269I GAATGC 2 cut(s) 1634, 4089
MvaI CCWGG 5 cut(s) 700, 921, 1272, 3258, 4032
MvnI CGCG 2 cut(s) 2236, 3372
NciI CCSGG 1 cut(s) 182
NcoI CCATGG 1 cut(s) 2064
NdeI CATATG 1 cut(s) 3507
NlaIV GGNNCC 5 cut(s) 1719, 2509, 2751, 2781, 2968
NmeAIII GCCGAG 2 cut(s) 262, 1561
NmuCI GTSAC 4 cut(s) 1463, 1470, 2097, 3796
NruI TCGCGA 1 cut(s) 2236
NsiI ATGCAT 2 cut(s) 1488, 2463
NspI RCATGY 1 cut(s) 2002
NspV TTCGAA 1 cut(s) 1032
OliI CACNNNNGTG 1 cut(s) 1461
PaeI GCATGC 1 cut(s) 2002
PaeR7I CTCGAG 2 cut(s) 206, 3428
PagI TCATGA 1 cut(s) 2281
PasI CCCWGGG 1 cut(s) 1271
PciSI GCTCTTC 1 cut(s) 1848
PctI GAATGC 2 cut(s) 1634, 4089
PdmI GAANNNNTTC 2 cut(s) 2841, 3324
PflMI CCANNNNNTGG 2 cut(s) 2417, 3770
PfoI TCCNGGA 1 cut(s) 180
Ple19I CGATCG 1 cut(s) 1919
PleI GAGTC 7 cut(s) 179, 391, 493, 905, 1740, 3084, 3568
PmaCI CACGTG 1 cut(s) 2663
PmlI CACGTG 1 cut(s) 2663
PpsI GAGTC 7 cut(s) 179, 391, 493, 905, 1740, 3084, 3568
Ppu21I YACGTR 1 cut(s) 2663
PpuMI RGGWCCY 1 cut(s) 1443
PshBI ATTAAT 1 cut(s) 1898
Psp124BI GAGCTC 1 cut(s) 3201
Psp5II RGGWCCY 1 cut(s) 1443
Psp6I CCWGG 5 cut(s) 698, 919, 1270, 3256, 4030
PspCI CACGTG 1 cut(s) 2663
PspFI CCCAGC 3 cut(s) 882, 2609, 3920
PspGI CCWGG 5 cut(s) 698, 919, 1270, 3256, 4030
PspN4I GGNNCC 5 cut(s) 1719, 2509, 2751, 2781, 2968
PspPI GGNCC 2 cut(s) 1443, 4287
PspPPI RGGWCCY 1 cut(s) 1443
PspXI VCTCGAGB 1 cut(s) 206
PsrI GAACNNNNNNTAC 2 cut(s) 2215, 2247
PstI CTGCAG 4 cut(s) 1893, 1939, 2277, 3297
PstNI CAGNNNCTG 2 cut(s) 3916, 3947
PsuI RGATCY 4 cut(s) 1604, 2440, 2966, 4105
PvuI CGATCG 1 cut(s) 1919
PvuII CAGCTG 1 cut(s) 811
RruI TCGCGA 1 cut(s) 2236
RsaI GTAC 8 cut(s) 2224, 2262, 2323, 2372, 2929, 2936, 3210, 3584
RsaNI GTAC 8 cut(s) 2223, 2261, 2322, 2371, 2928, 2935, 3209, 3583
RseI CAYNNNNRTG 5 cut(s) 528, 1461, 2193, 2363, 2382
SacI GAGCTC 1 cut(s) 3201
SapI GCTCTTC 1 cut(s) 1848
Sau96I GGNCC 2 cut(s) 1443, 4287
ScaI AGTACT 2 cut(s) 2224, 3584
SchI GAGTC 7 cut(s) 179, 392, 493, 906, 1740, 3085, 3568
ScrFI CCNGG 6 cut(s) 182, 700, 921, 1272, 3258, 4032
SduI GDGCHC 4 cut(s) 19, 919, 3201, 3385
SfcI CTRYAG 6 cut(s) 1889, 1935, 2273, 2754, 2784, 3293
Sfr274I CTCGAG 2 cut(s) 206, 3428
SfuI TTCGAA 1 cut(s) 1032
SinI GGWCC 2 cut(s) 1443, 4287
SlaI CTCGAG 2 cut(s) 206, 3428
SmiMI CAYNNNNRTG 5 cut(s) 528, 1461, 2193, 2363, 2382
SpeI ACTAGT 1 cut(s) 748
SphI GCATGC 1 cut(s) 2002
SsiI CCGC 6 cut(s) 149, 465, 759, 1753, 2703, 3370
SspI AATATT 3 cut(s) 1975, 3104, 3218
SstI GAGCTC 1 cut(s) 3201
StyD4I CCNGG 6 cut(s) 180, 698, 919, 1270, 3256, 4030
StyI CCWWGG 3 cut(s) 2064, 2416, 3726
TaiI ACGT 3 cut(s) 216, 379, 2665
TaqII GACCGA 1 cut(s) 3132
TatI WGTACW 4 cut(s) 2222, 2927, 3208, 3582
TscAI CASTG 8 cut(s) 1018, 1468, 1645, 2152, 2277, 2833, 3546, 3983
TseFI GTSAC 4 cut(s) 1463, 1470, 2097, 3796
Tsp45I GTSAC 4 cut(s) 1463, 1470, 2097, 3796
TspGWI ACGGA 3 cut(s) 501, 1411, 2270
TspRI CASTG 8 cut(s) 1018, 1468, 1645, 2152, 2277, 2833, 3546, 3983
Van91I CCANNNNNTGG 2 cut(s) 2417, 3770
Vha464I CTTAAG 1 cut(s) 3928
VneI GTGCAC 1 cut(s) 915
VpaK11BI GGWCC 2 cut(s) 1443, 4287
VspI ATTAAT 1 cut(s) 1898
XagI CCTNNNNNAGG 2 cut(s) 3398, 3752
XbaI TCTAGA 3 cut(s) 2677, 4003, 4249
XceI RCATGY 1 cut(s) 2002
XcmI CCANNNNNNNNNTGG 1 cut(s) 1268
XhoI CTCGAG 2 cut(s) 206, 3428
XmiI GTMKAC 1 cut(s) 1571
XmnI GAANNNNTTC 2 cut(s) 2841, 3324
ZraI GACGTC 1 cut(s) 214
ZrmI AGTACT 2 cut(s) 2224, 3584
Zsp2I ATGCAT 2 cut(s) 1488, 2463
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.