Rw0G005440

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00216
OG0004242
Physical Location & Seq
Reverse (-)
571 .. 3275
2705 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G005440.1

Sequence Viewer

Length: 2670 bp
ATGCCTATTGTTCGAGCACTTGAATCTGCTTCTGTAAGCATCCTCCTTAACAAGTTGGCCACTCAAGATGTCATAGATTTCTTCCTCAAGTGGAAAATCGATGATTCCCTGCGAGAAAAGCTCAAGAGAAACCTGCTTGTCATTCATGCAGTTCTCAATCATGCTGAGGAGAAGCAAGTCCGGGACTCAAATGTCAAAGCTTGGCTGGAGGACGTCAGAGTTGCAGCTTATGATGCCGAGGACATATTGGGTGACATCGCCAATGATGCTCTCGAATCACAAAACAGCAAACATGAGGTATGGAATTCTAAGATCAAAGAAGGGATAGACTTTAAGATCAAAGACATTGCTAATACTCTTAATCCCTTTAGGGAACGTGTAGAGTCGAAAATGATAAAGATTATAGATGTATTGGAAGAGATTGCAAAGCAGAAAGATGTTCTTCGTTTGAGAGAAGATTCTGGCGGTATAGAATCAGGTTTTGACGGATTGCCCACGACTCCAATGTTGGGTGATAAATCTCATATTTATGGTAGAGAATCTGATAAGAAGGAGATAATTGCTTTGCTGAAATCGGGTAAAAATGATCGTGAGGTATCTATAATTCCAATTGTAGGCATGGGAGGCATTGGGAAGACTACTCTTGCTCAGCTTGTGTATAATGATGCCAAAGTGAGTAGCTATTTTAATTTGAGAGCATGGGCTTGTGTCTCTGATGTATTTGATGTGCAAAGGATAACAAAAACACTAGTTGAGTCCGCCACAAAATCAAGTTGTAGCACAAACAATTTGGAGTTACTTCAAGAACAGCTGAAAATACGGTTGAAGAACAAGAAATTTCTTTTTGTTCTGGATGATGTCTGGAACGACAAGTATGAAAGCTGGGATAAGTTAAGAGTCCCCTTCACAGTTGGTGCACCAGGGAGTAAAATCATAATCACGACACGAAACAAAACAGTTGCATCACTTATGGGGACTGTTCCTACTTACTGTTTGGAGGGGCTTTCGGACAGTGATTGTTGGTTATTATTCGAACAAATTTTGAAAGTGATTGGCAAGAAAATAGCATACAAGTGTAAAGGGTTGCCTTTGGCTGCAAAGGCACTAGGAGGTCTCCTGCGTGCTGAACCGGAACTAGATGAGAATTACTGGAATGATATCTTGAATAGCAAACTTTGGAAACTGGCAGACAACACTATTCTTCCAGCTCTAAGATTAAGCTACCATCACCTCCCTGGGCATTTGAAGCGCTGCTTTGCTTATTGCTCGATGTTTCCTAAGGACTATGAATATGAGAGAGAGATGTTAATCTTGTTATGGATGGCAGAGGGTTTTGTGCCAGAGCCAGAAGGAAATCAACGGATCGAAGATGTAGCTGGAAGATATGTTTCAGATTTTTTATCAAGGTCCTTCTTCCAACACACCAGTGACAAGTCACGACTGGTAATGCATGACCTCATACATGATCTTGCACAATCTGTTTCTGGCAAAACATTTGTTAGGTTGGAGGAAAATTCAGAGAACCATAAAAAGTCTACAAAGGCTCGGCACTTGTCATATACCCGTGGATCTGAAGATGTATTTCAGAAATTTGAGGCATTCAGTGAGGTGGATTGTTTACGAACCTTTCTACCGCTAGACCCGTTGCAAGGATTCAATGTGAGTGGCTTATCTGACAAGGTTCCTTGTGATATGATGCCAAAATTAAGACTCCTGCGGGTGCTATCCTTTAGTGGTTATCTCATAAAAAAGTTGCCAGATTCAATTGGCAACTTGAGGCATCTGCGATACCTCAATCTTTCTCATTGTCAAATAGAAGAGCTACCTGAGCCAACAAGCAGTCTATACAATCTGCAGACATTAATTTTATTCAGTTGTCGATCGCTTACCATGCTGCCTGCAGACATGGGAAACCTAAGGAATTTACGGCATCTCAATATTCAGGGAACTAGGCTGAAAAGTATGCCTTTGGGAATGGGAAGATTGGTTAACCTTCAAATACTGTCAGATTTTATTGTGGGGAAAGCCATGGGAAAAGGGATTGCAGAATTGAAGGACTTGTCACATCTTCGAGGCTCAGTTTCTATTTTGTGTTTGCATGATGTCGGCAGTGTTAGGGATGCAATTGAGGCCAGATTAGAGGTTAAGAAGCACCTTGATGATTTAGTTTTGGAATGGAGCAGCAGTACTGATGGTTCGCGAAATGAAGAGATCGAGACAGAGGTACTTGATGCACTGCAGCCTCATGAAAACCTGAAGAAACTCACCATTAAGTATTATGGAGGTACAGAATTTCCATATTGGATGGGGAATCCTTTGTTCACTAACATGGCGTACTTGCATCTCTATGGTTGCATAAAATGCACCTCTTTACCACCCCTTGGCCAACTACCTTCTCTTAAAGATCTCATAATTGAAGCAATGCATGGAATACAGCGTGTAGGAATGGATTTTTTTGGGGATGATGATGGTTCCATAATTTTTTTCCCCTCTTTAGAGACTTTGAAATTTGAGGACATGAAAGAATGGGAGAAATGGTCATCTTCAGAAGATGAAAGGCTTAAAGGATTTCCCAGCCTTCGGGAGCTTTCCATATTTAGATGTCCCAAGTTAACAAAGTTTACACATGGATTTGCTTCTCTAGAAAAGCTGCGCATCAAGAACTGCAGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

890

Amino Acids

101.18

Weight (kDa)

6.9

Isoelectric Point (pI)

43.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 12 - 98 1.4e-15 Rx N-terminal domain
NB-ARC PF00931 181 - 347 9.4e-34 NB-ARC domain
WHD_DRP PF23559 424 - 491 5.9e-25 Disease resistance protein Winged helix domain
LRR_14 PF23598 566 - 841 1.2e-30 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 681 - 808 3.6e-40 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 191
AatII GACGTC 1 cut(s) 216
Acc16I TGCGCA 1 cut(s) 2654
Acc36I ACCTGC 1 cut(s) 141
AccB7I CCANNNNNTGG 1 cut(s) 2381
AccI GTMKAC 1 cut(s) 1535
AccII CGCG 1 cut(s) 2200
AciI CCGC 4 cut(s) 465, 759, 1634, 1717
AclWI GGATC 2 cut(s) 1370, 1576
AcoI YGGCCR 2 cut(s) 57, 2383
AcsI RAATTY 8 cut(s) 304, 836, 1038, 1513, 1589, 1921, 2291, 2507
AcuI CTGAAG 3 cut(s) 1593, 2276, 2529
AcyI GRCGYC 1 cut(s) 213
AfaI GTAC 4 cut(s) 2188, 2226, 2287, 2336
AfeI AGCGCT 1 cut(s) 1250
AfiI CCNNNNNNNGG 5 cut(s) 509, 614, 1649, 2381, 2580
AflIII ACRYGT 1 cut(s) 376
AhlI ACTAGT 1 cut(s) 748
AjnI CCWGG 2 cut(s) 919, 1234
AleI CACNNNNGTG 1 cut(s) 1425
Alw21I GWGCWC 2 cut(s) 19, 919
Alw26I GTCTC 4 cut(s) 715, 1118, 2210, 2492
Alw44I GTGCAC 1 cut(s) 915
AlwI GGATC 2 cut(s) 1370, 1576
Aor51HI AGCGCT 1 cut(s) 1250
AoxI GGCC 3 cut(s) 57, 2130, 2383
ApaLI GTGCAC 1 cut(s) 915
ApeKI GCWGC 7 cut(s) 224, 1094, 1251, 1894, 2181, 2239, 2650
ApoI RAATTY 8 cut(s) 304, 836, 1038, 1513, 1589, 1921, 2291, 2507
AseI ATTAAT 1 cut(s) 1862
AspLEI GCGC 2 cut(s) 1251, 2655
AspS9I GGNCC 1 cut(s) 1407
AsuC2I CCSGG 1 cut(s) 182
AsuHPI GGTGA 4 cut(s) 263, 524, 1220, 2257
AsuII TTCGAA 1 cut(s) 1032
AvaII GGWCC 1 cut(s) 1407
AxyI CCTNAGG 2 cut(s) 1278, 1916
BaeGI GKGCMC 1 cut(s) 919
BalI TGGCCA 2 cut(s) 59, 2385
BbsI GAAGAC 1 cut(s) 641
Bbv12I GWGCWC 2 cut(s) 19, 919
BbvCI CCTCAGC 1 cut(s) 165
BbvI GCAGC 7 cut(s) 236, 1081, 1238, 1881, 2193, 2251, 2637
BccI CCATC 5 cut(s) 1233, 1315, 2186, 2299, 2462
BceAI ACGGC 1 cut(s) 1943
BciT130I CCWGG 2 cut(s) 921, 1236
BcnI CCSGG 1 cut(s) 182
BcoDI GTCTC 4 cut(s) 715, 1118, 2210, 2492
BcuI ACTAGT 1 cut(s) 748
BfaI CTAG 6 cut(s) 749, 1106, 1136, 1637, 1950, 2642
BfmI CTRYAG 4 cut(s) 1853, 1899, 2237, 2665
BfoI RGCGCY 1 cut(s) 1252
BfuAI ACCTGC 1 cut(s) 141
BglII AGATCT 1 cut(s) 2404
BisI GCNGC 7 cut(s) 225, 1095, 1252, 1895, 2182, 2240, 2651
BlpI GCTNAGC 1 cut(s) 648
BlsI GCNGC 7 cut(s) 226, 1096, 1253, 1896, 2183, 2241, 2652
BmcAI AGTACT 1 cut(s) 2188
Bme1390I CCNGG 3 cut(s) 182, 921, 1236
Bme18I GGWCC 1 cut(s) 1407
BmgT120I GGNCC 1 cut(s) 1407
BmiI GGNNCC 2 cut(s) 1683, 2473
BmrFI CCNGG 3 cut(s) 182, 921, 1236
BpiI GAAGAC 1 cut(s) 641
BplI GAGNNNNNCTC 2 cut(s) 105, 137
BpmI CTGGAG 1 cut(s) 227
Bpu10I CCTNAGC 2 cut(s) 165, 1827
Bpu1102I GCTNAGC 1 cut(s) 648
Bpu14I TTCGAA 1 cut(s) 1032
BpuEI CTTGAG 4 cut(s) 48, 71, 107, 1795
BpuMI CCSGG 1 cut(s) 182
Bsa29I ATCGAT 1 cut(s) 99
BsaBI GATNNNNATC 1 cut(s) 1307
BsaHI GRCGYC 1 cut(s) 213
BsaI GGTCTC 1 cut(s) 1118
BsaJI CCNNGG 7 cut(s) 237, 920, 1234, 1235, 1564, 2028, 2380
BsaWI WCCGGW 1 cut(s) 1129
BsaXI ACNNNNNCTCC 4 cut(s) 2170, 2200, 2522, 2552
Bsc4I CCNNNNNNNGG 5 cut(s) 509, 614, 1649, 2381, 2580
Bse1I ACTGG 4 cut(s) 1154, 1188, 1425, 1446
Bse21I CCTNAGG 2 cut(s) 1278, 1916
Bse3DI GCAATG 2 cut(s) 345, 2427
Bse8I GATNNNNATC 1 cut(s) 1307
BseBI CCWGG 2 cut(s) 921, 1236
BseCI ATCGAT 1 cut(s) 99
BseDI CCNNGG 7 cut(s) 237, 920, 1234, 1235, 1564, 2028, 2380
BseGI GGATG 6 cut(s) 39, 859, 1326, 2125, 2310, 2467
BseJI GATNNNNATC 1 cut(s) 1307
BseLI CCNNNNNNNGG 5 cut(s) 509, 614, 1649, 2381, 2580
BseMI GCAATG 2 cut(s) 345, 2427
BseMII CTCAG 4 cut(s) 156, 662, 1818, 2091
BseNI ACTGG 4 cut(s) 1154, 1188, 1425, 1446
BseRI GAGGAG 1 cut(s) 182
BseSI GKGCMC 1 cut(s) 919
BseXI GCAGC 7 cut(s) 236, 1081, 1238, 1881, 2193, 2251, 2637
BseYI CCCAGC 2 cut(s) 882, 2573
Bsh1236I CGCG 1 cut(s) 2200
Bsh1285I CGRYCG 1 cut(s) 1883
BshFI GGCC 3 cut(s) 59, 2132, 2385
BshVI ATCGAT 1 cut(s) 99
BsiEI CGRYCG 1 cut(s) 1883
BsiHKAI GWGCWC 2 cut(s) 19, 919
BsiSI CCGG 2 cut(s) 181, 1130
BslFI GGGAC 4 cut(s) 197, 884, 988, 2589
BslI CCNNNNNNNGG 5 cut(s) 509, 614, 1649, 2381, 2580
BsmAI GTCTC 4 cut(s) 715, 1118, 2210, 2492
BsmFI GGGAC 4 cut(s) 197, 884, 988, 2589
BsmI GAATGC 1 cut(s) 1598
BsnI GGCC 3 cut(s) 59, 2132, 2385
Bso31I GGTCTC 1 cut(s) 1118
Bsp119I TTCGAA 1 cut(s) 1032
Bsp1286I GDGCHC 2 cut(s) 19, 919
Bsp143I GATC 9 cut(s) 312, 336, 586, 1362, 1465, 1568, 1880, 2211, 2404
Bsp1720I GCTNAGC 1 cut(s) 648
Bsp19I CCATGG 1 cut(s) 2028
Bsp68I TCGCGA 1 cut(s) 2200
BspACI CCGC 4 cut(s) 465, 759, 1634, 1717
BspANI GGCC 3 cut(s) 59, 2132, 2385
BspCNI CTCAG 4 cut(s) 157, 661, 1819, 2090
BspDI ATCGAT 1 cut(s) 99
BspFNI CGCG 1 cut(s) 2200
BspHI TCATGA 1 cut(s) 2245
BspLI GGNNCC 2 cut(s) 1683, 2473
BspMAI CTGCAG 4 cut(s) 1857, 1903, 2241, 2669
BspMI ACCTGC 1 cut(s) 141
BspPI GGATC 2 cut(s) 1370, 1576
BspQI GCTCTTC 1 cut(s) 1812
BspT104I TTCGAA 1 cut(s) 1032
BspTNI GGTCTC 1 cut(s) 1118
BsrDI GCAATG 2 cut(s) 345, 2427
BsrI ACTGG 4 cut(s) 1154, 1188, 1425, 1446
BssECI CCNNGG 7 cut(s) 237, 920, 1234, 1235, 1564, 2028, 2380
BssMI GATC 9 cut(s) 312, 336, 586, 1362, 1465, 1568, 1880, 2211, 2404
BssNI GRCGYC 1 cut(s) 213
BssT1I CCWWGG 2 cut(s) 2028, 2380
Bst2UI CCWGG 2 cut(s) 921, 1236
Bst4CI ACNGT 7 cut(s) 822, 910, 958, 979, 992, 1013, 2004
Bst6I CTCTTC 3 cut(s) 411, 1812, 2202
BstACI GRCGYC 1 cut(s) 213
BstAPI GCANNNNNTGC 1 cut(s) 2361
BstBI TTCGAA 1 cut(s) 1032
BstC8I GCNNGC 2 cut(s) 1122, 1899
BstDEI CTNAG 8 cut(s) 165, 309, 648, 1211, 1278, 1827, 1916, 2077
BstDSI CCRYGG 2 cut(s) 1564, 2028
BstF5I GGATG 6 cut(s) 39, 859, 1326, 2125, 2310, 2467
BstFNI CGCG 1 cut(s) 2200
BstH2I RGCGCY 1 cut(s) 1252
BstHHI GCGC 2 cut(s) 1251, 2655
BstKTI GATC 9 cut(s) 315, 339, 589, 1365, 1468, 1571, 1883, 2214, 2407
BstMAI GTCTC 4 cut(s) 715, 1118, 2210, 2492
BstMBI GATC 9 cut(s) 312, 336, 586, 1362, 1465, 1568, 1880, 2211, 2404
BstMCI CGRYCG 1 cut(s) 1883
BstNI CCWGG 2 cut(s) 921, 1236
BstSCI CCNGG 3 cut(s) 180, 919, 1234
BstSFI CTRYAG 4 cut(s) 1853, 1899, 2237, 2665
BstSLI GKGCMC 1 cut(s) 919
BstUI CGCG 1 cut(s) 2200
BstV1I GCAGC 7 cut(s) 236, 1081, 1238, 1881, 2193, 2251, 2637
BstV2I GAAGAC 1 cut(s) 641
BstX2I RGATCY 2 cut(s) 1568, 2404
BstYI RGATCY 2 cut(s) 1568, 2404
Bsu15I ATCGAT 1 cut(s) 99
Bsu36I CCTNAGG 2 cut(s) 1278, 1916
BsuRI GGCC 3 cut(s) 59, 2132, 2385
BsuTUI ATCGAT 1 cut(s) 99
BtgI CCRYGG 2 cut(s) 1564, 2028
BtgZI GCGATG 1 cut(s) 241
BtsCI GGATG 6 cut(s) 39, 859, 1326, 2125, 2310, 2467
BtsI GCAGTG 2 cut(s) 2116, 2234
BtsIMutI CAGTG 5 cut(s) 1018, 1432, 1609, 2116, 2234
BtuMI TCGCGA 1 cut(s) 2200
BveI ACCTGC 1 cut(s) 141
Cac8I GCNNGC 2 cut(s) 1122, 1899
CciI TCATGA 1 cut(s) 2245
CfoI GCGC 2 cut(s) 1251, 2655
Cfr13I GGNCC 1 cut(s) 1407
ClaI ATCGAT 1 cut(s) 99
Csp6I GTAC 4 cut(s) 2187, 2225, 2286, 2335
CspCI CAANNNNNGTGG 2 cut(s) 1645, 1680
CviQI GTAC 4 cut(s) 2187, 2225, 2286, 2335
DdeI CTNAG 8 cut(s) 165, 309, 648, 1211, 1278, 1827, 1916, 2077
DpnI GATC 9 cut(s) 314, 338, 588, 1364, 1467, 1570, 1882, 2213, 2406
DpnII GATC 9 cut(s) 312, 336, 586, 1362, 1465, 1568, 1880, 2211, 2404
DrdI GACNNNNNNGTC 1 cut(s) 191
DseDI GACNNNNNNGTC 1 cut(s) 191
EaeI YGGCCR 2 cut(s) 57, 2383
Eam1104I CTCTTC 3 cut(s) 411, 1812, 2202
EarI CTCTTC 3 cut(s) 411, 1812, 2202
EciI GGCGGA 1 cut(s) 748
Eco130I CCWWGG 2 cut(s) 2028, 2380
Eco31I GGTCTC 1 cut(s) 1118
Eco32I GATATC 1 cut(s) 1159
Eco47I GGWCC 1 cut(s) 1407
Eco47III AGCGCT 1 cut(s) 1250
Eco57I CTGAAG 3 cut(s) 1593, 2276, 2529
Eco81I CCTNAGG 2 cut(s) 1278, 1916
EcoO109I RGGNCCY 1 cut(s) 1407
EcoRI GAATTC 1 cut(s) 304
EcoRII CCWGG 2 cut(s) 919, 1234
EcoRV GATATC 1 cut(s) 1159
EcoT14I CCWWGG 2 cut(s) 2028, 2380
EcoT22I ATGCAT 2 cut(s) 1452, 2427
ErhI CCWWGG 2 cut(s) 2028, 2380
FalI AAGNNNNNCTT 6 cut(s) 426, 458, 1238, 1270, 1951, 1983
FaqI GGGAC 4 cut(s) 197, 884, 988, 2589
FauI CCCGC 1 cut(s) 1710
FblI GTMKAC 1 cut(s) 1535
Fnu4HI GCNGC 7 cut(s) 225, 1095, 1252, 1895, 2182, 2240, 2651
FokI GGATG 6 cut(s) 26, 866, 1333, 2132, 2317, 2474
Fsp4HI GCNGC 7 cut(s) 225, 1095, 1252, 1895, 2182, 2240, 2651
FspBI CTAG 6 cut(s) 749, 1106, 1136, 1637, 1950, 2642
FspI TGCGCA 1 cut(s) 2654
GlaI GCGC 2 cut(s) 1250, 2654
GluI GCNGC 7 cut(s) 225, 1095, 1252, 1895, 2182, 2240, 2651
GsaI CCCAGC 2 cut(s) 886, 2577
GsuI CTGGAG 1 cut(s) 227
HaeII RGCGCY 1 cut(s) 1252
HaeIII GGCC 3 cut(s) 59, 2132, 2385
HapII CCGG 2 cut(s) 181, 1130
HhaI GCGC 2 cut(s) 1251, 2655
Hin1I GRCGYC 1 cut(s) 213
Hin6I GCGC 2 cut(s) 1249, 2653
HinP1I GCGC 2 cut(s) 1249, 2653
HincII GTYRAC 2 cut(s) 1990, 2613
HindII GTYRAC 2 cut(s) 1990, 2613
HindIII AAGCTT 1 cut(s) 198
HpaI GTTAAC 2 cut(s) 1990, 2613
HpaII CCGG 2 cut(s) 181, 1130
HphI GGTGA 4 cut(s) 263, 524, 1220, 2257
Hpy166II GTNNAC 7 cut(s) 917, 1536, 1619, 1990, 2322, 2613, 2622
Hpy8I GTNNAC 7 cut(s) 917, 1536, 1619, 1990, 2322, 2613, 2622
HpyAV CCTTC 9 cut(s) 314, 544, 913, 1343, 1420, 2003, 2047, 2403, 2588
HpyCH4III ACNGT 7 cut(s) 822, 910, 958, 979, 992, 1013, 2004
HpyCH4IV ACGT 2 cut(s) 213, 376
HpyF3I CTNAG 8 cut(s) 165, 309, 648, 1211, 1278, 1827, 1916, 2077
HpySE526I ACGT 2 cut(s) 213, 376
Hsp92I GRCGYC 1 cut(s) 213
HspAI GCGC 2 cut(s) 1249, 2653
KspAI GTTAAC 2 cut(s) 1990, 2613
Kzo9I GATC 9 cut(s) 312, 336, 586, 1362, 1465, 1568, 1880, 2211, 2404
LguI GCTCTTC 1 cut(s) 1812
LmnI GCTCC 2 cut(s) 2178, 2584
Lsp1109I GCAGC 7 cut(s) 236, 1081, 1238, 1881, 2193, 2251, 2637
MaeI CTAG 6 cut(s) 749, 1106, 1136, 1637, 1950, 2642
MaeII ACGT 2 cut(s) 213, 376
MaeIII GTNAC 5 cut(s) 251, 795, 1427, 1434, 2061
MalI GATC 9 cut(s) 314, 338, 588, 1364, 1467, 1570, 1882, 2213, 2406
MboI GATC 9 cut(s) 312, 336, 586, 1362, 1465, 1568, 1880, 2211, 2404
MfeI CAATTG 3 cut(s) 609, 1764, 2124
MflI RGATCY 2 cut(s) 1568, 2404
MhlI GDGCHC 2 cut(s) 19, 919
MlsI TGGCCA 2 cut(s) 59, 2385
MluNI TGGCCA 2 cut(s) 59, 2385
MlyI GAGTC 6 cut(s) 179, 392, 493, 764, 906, 1704
MmeI TCCRAC 2 cut(s) 1441, 1485
Mox20I TGGCCA 2 cut(s) 59, 2385
Mph1103I ATGCAT 2 cut(s) 1452, 2427
MscI TGGCCA 2 cut(s) 59, 2385
MslI CAYNNNNRTG 6 cut(s) 528, 1072, 1425, 2157, 2327, 2346
Msp20I TGGCCA 2 cut(s) 59, 2385
MspA1I CMGCKG 1 cut(s) 811
MspI CCGG 2 cut(s) 181, 1130
MspR9I CCNGG 3 cut(s) 182, 921, 1236
MunI CAATTG 3 cut(s) 609, 1764, 2124
Mva1269I GAATGC 1 cut(s) 1598
MvaI CCWGG 2 cut(s) 921, 1236
MvnI CGCG 1 cut(s) 2200
NciI CCSGG 1 cut(s) 182
NcoI CCATGG 1 cut(s) 2028
NdeII GATC 9 cut(s) 312, 336, 586, 1362, 1465, 1568, 1880, 2211, 2404
NlaIV GGNNCC 2 cut(s) 1683, 2473
NmeAIII GCCGAG 2 cut(s) 262, 1525
NmuCI GTSAC 4 cut(s) 251, 1427, 1434, 2061
NruI TCGCGA 1 cut(s) 2200
NsbI TGCGCA 1 cut(s) 2654
NsiI ATGCAT 2 cut(s) 1452, 2427
NspV TTCGAA 1 cut(s) 1032
OliI CACNNNNGTG 1 cut(s) 1425
PagI TCATGA 1 cut(s) 2245
PasI CCCWGGG 1 cut(s) 1235
PciSI GCTCTTC 1 cut(s) 1812
PctI GAATGC 1 cut(s) 1598
PfeI GAWTC 9 cut(s) 23, 104, 275, 458, 473, 539, 1653, 1760, 2311
PflMI CCANNNNNTGG 1 cut(s) 2381
PfoI TCCNGGA 1 cut(s) 180
PkrI GCNGC 7 cut(s) 226, 1096, 1253, 1896, 2183, 2241, 2652
Ple19I CGATCG 1 cut(s) 1883
PleI GAGTC 6 cut(s) 179, 391, 493, 763, 905, 1704
PpsI GAGTC 6 cut(s) 179, 391, 493, 763, 905, 1704
PpuMI RGGWCCY 1 cut(s) 1407
PshBI ATTAAT 1 cut(s) 1862
Psp5II RGGWCCY 1 cut(s) 1407
Psp6I CCWGG 2 cut(s) 919, 1234
PspFI CCCAGC 2 cut(s) 882, 2573
PspGI CCWGG 2 cut(s) 919, 1234
PspN4I GGNNCC 2 cut(s) 1683, 2473
PspPI GGNCC 1 cut(s) 1407
PspPPI RGGWCCY 1 cut(s) 1407
PsrI GAACNNNNNNTAC 2 cut(s) 2179, 2211
PstI CTGCAG 4 cut(s) 1857, 1903, 2241, 2669
PsuI RGATCY 2 cut(s) 1568, 2404
PvuI CGATCG 1 cut(s) 1883
PvuII CAGCTG 1 cut(s) 811
RruI TCGCGA 1 cut(s) 2200
RsaI GTAC 4 cut(s) 2188, 2226, 2287, 2336
RsaNI GTAC 4 cut(s) 2187, 2225, 2286, 2335
RseI CAYNNNNRTG 6 cut(s) 528, 1072, 1425, 2157, 2327, 2346
SapI GCTCTTC 1 cut(s) 1812
SatI GCNGC 7 cut(s) 225, 1095, 1252, 1895, 2182, 2240, 2651
Sau3AI GATC 9 cut(s) 312, 336, 586, 1362, 1465, 1568, 1880, 2211, 2404
Sau96I GGNCC 1 cut(s) 1407
ScaI AGTACT 1 cut(s) 2188
SchI GAGTC 6 cut(s) 179, 392, 493, 764, 906, 1704
ScrFI CCNGG 3 cut(s) 182, 921, 1236
SduI GDGCHC 2 cut(s) 19, 919
SfcI CTRYAG 4 cut(s) 1853, 1899, 2237, 2665
SfuI TTCGAA 1 cut(s) 1032
SinI GGWCC 1 cut(s) 1407
SmiMI CAYNNNNRTG 6 cut(s) 528, 1072, 1425, 2157, 2327, 2346
SmlI CTYRAG 4 cut(s) 63, 86, 122, 1774
SmoI CTYRAG 4 cut(s) 63, 86, 122, 1774
SpeI ACTAGT 1 cut(s) 748
SsiI CCGC 4 cut(s) 465, 759, 1634, 1717
SspI AATATT 1 cut(s) 1939
SspMI CTAG 6 cut(s) 749, 1106, 1136, 1637, 1950, 2642
StyD4I CCNGG 3 cut(s) 180, 919, 1234
StyI CCWWGG 2 cut(s) 2028, 2380
TaaI ACNGT 7 cut(s) 822, 910, 958, 979, 992, 1013, 2004
TaiI ACGT 2 cut(s) 216, 379
TatI WGTACW 1 cut(s) 2186
TfiI GAWTC 9 cut(s) 23, 104, 275, 458, 473, 539, 1653, 1760, 2311
TscAI CASTG 5 cut(s) 1018, 1432, 1609, 2116, 2241
TseFI GTSAC 4 cut(s) 251, 1427, 1434, 2061
TseI GCWGC 7 cut(s) 224, 1094, 1251, 1894, 2181, 2239, 2650
Tsp45I GTSAC 4 cut(s) 251, 1427, 1434, 2061
TspDTI ATGAA 7 cut(s) 134, 891, 1302, 2220, 2262, 2534, 2568
TspGWI ACGGA 2 cut(s) 501, 1375
TspRI CASTG 5 cut(s) 1018, 1432, 1609, 2116, 2241
Van91I CCANNNNNTGG 1 cut(s) 2381
VneI GTGCAC 1 cut(s) 915
VpaK11BI GGWCC 1 cut(s) 1407
VspI ATTAAT 1 cut(s) 1862
XapI RAATTY 8 cut(s) 304, 836, 1038, 1513, 1589, 1921, 2291, 2507
XbaI TCTAGA 1 cut(s) 2641
XcmI CCANNNNNNNNNTGG 1 cut(s) 1232
XmiI GTMKAC 1 cut(s) 1535
XspI CTAG 6 cut(s) 749, 1106, 1136, 1637, 1950, 2642
ZraI GACGTC 1 cut(s) 214
ZrmI AGTACT 1 cut(s) 2188
Zsp2I ATGCAT 2 cut(s) 1452, 2427
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.