Rh1DG034000

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0004242
Physical Location & Seq
Reverse (-)
4965065 .. 4969604
4540 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG034000.1

Sequence Viewer

Length: 1695 bp
ATGCCTATTGTTCGAGCACTTGAATCTGCTTCTGTAAGCATCCTCCTTAACAAGTTGGCCACTCAAGATGTCATAGATTTCTTCCTCAAGTGGAAAATTGATGACTCCCTGCGAGAAAAGCTCAAGAGAAACCTGCTTGTCATTCATGCAGTTCTCAATCATGCTGAGGAGAAGCAAGTCCGGGACTCAAATGTCAAAGCTTGGCTCGAGGACGTCAGAGTTGCAGCTTATGATGCCGAGGACATATTGGGTGACATCGCCAATGATGCTCTCGAATCACAAAACAGCAAACATGAGGTATGGAATTCTAAGATCAAAGAAGGGATAGACTTTAAGATGAAAGACATTGCTAATACTCTTAATCCCTTTAGGGAACGTGTAGAGTCGAAAATGATAAAGATTATAGATGTATTGGAAGAGATTGCAAAGCAGAAAGATGTTCTTCGTTTGAGAGAAGATTCTGGCGGTATAGAATCAGGTTTTGACGGATTGCCCACGACTCCAATGTTGGGTGATGAATCTCATGTTTATGGTAGAGAATCTGATAAGAAGGAGATAATTACTTTGCTGAAATCGGATGACCGAGTTGGACTAGCACACCTTGTATCCCTTCAACGCTTGACAATATCCCATTGTCCTAAGTTTGTTGCTTTGCCTGATGAGCTCCCACCTGTACTTAAATATTTGGATTTGAAACATTGTCCTAGCCTGATGAAGCTGCCTGGTAAACTTCACAACCTTGAGTGTCTTGCAGAGCTGCAGATTGAATGGTGTTCAAAGATTGAAGCATTTCCAGATGTGGGGTTGCCTGACAAACTGAAGCGTCTTCTAATCCGCGAATGTGGTGCTCTGAAGACCCTTCCTCAGGAGCTTCTCTGCAATAACAATTTTCTCGAGTATTTAGAAGTTCATAAATGTCCTTCTCTTGTGTCAGTTCTTGAAGAAGGAAACTTGCCATCAACTCTAACACATATGAAATTTTACTATTGCAAAATTCTGGGATCATTGCCTGAGGGTTTGATGCACAAAGACAATATGACTCTAAAGTACTTGGAGATAGACAACTGTTCCTCTCTCATGTCCTTTCCAAATGGTGAGTTACCAACAAGCCTTGAGCGTCTTGAGATTAGTGATTGCTCTAATTTCCAGACATTGCCATTGAGCCTTCTCAACCTCACGAATCTTGAGACCTTGGAAGTAAAGAGCTGCCCCCTTCTTCAGGACTTTCCAGAGGGTGGATTGCCACCCAACATAAAGTTTGTCACAATTTCCGAATGTAAGAATCTCAAGTCTCTACCTAAATTCTTTCATAAACTCAAATGTCTTCAGAAGTTGGAGATTTCTGGCTGTCCTAGTCTCCTGTCCTTGCCAAAGCAGGGTCTGCCCGGCAGCTTAAGACTACTTACAGTTACTGATTGTGAAAAGCTTAATCCCATTCATCAGTGGAGACTTCACAAACTACCCTCTCTAGATGTTTTCCCCATTGGTGGATTTCCTGGCCTGAAGTCCTTTTCAAAAGATTATCCTCTTCCTCACACTTTAACCTCTTTAAGCATTCAAAGATTCCCAGATCTTGAATCCATATCGGAGATCATTAGAAACAGAGAAGTTGTGTCAGTTACTACATCTTTCATGATCAATCCAGAAGTACTTGCTAGATCCCATTCTGTACCAGAATCTATAGCTGGAGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

564

Amino Acids

63.52

Weight (kDa)

5.95

Isoelectric Point (pI)

49.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 12 - 98 9.2e-16 Rx N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 191
AatII GACGTC 1 cut(s) 216
Acc36I ACCTGC 1 cut(s) 141
AccB7I CCANNNNNTGG 1 cut(s) 1235
AccII CGCG 1 cut(s) 837
AciI CCGC 2 cut(s) 465, 835
AclWI GGATC 2 cut(s) 1009, 1653
AcoI YGGCCR 1 cut(s) 57
AcsI RAATTY 4 cut(s) 304, 977, 993, 1301
AcuI CTGAAG 5 cut(s) 839, 872, 1202, 1310, 1523
AcyI GRCGYC 1 cut(s) 213
AfaI GTAC 4 cut(s) 675, 1049, 1650, 1671
AfiI CCNNNNNNNGG 7 cut(s) 509, 800, 865, 1219, 1235, 1376, 1487
AflII CTTAAG 1 cut(s) 1393
AflIII ACRYGT 1 cut(s) 376
AjnI CCWGG 2 cut(s) 721, 1495
Alw21I GWGCWC 3 cut(s) 19, 666, 850
Alw26I GTCTC 4 cut(s) 1181, 1296, 1361, 1441
AlwI GGATC 2 cut(s) 1009, 1653
AlwNI CAGNNNCTG 2 cut(s) 1381, 1412
Ama87I CYCGRG 2 cut(s) 206, 893
AoxI GGCC 2 cut(s) 57, 1498
ApeKI GCWGC 5 cut(s) 224, 718, 757, 1206, 1389
ApoI RAATTY 4 cut(s) 304, 977, 993, 1301
Asp700I GAANNNNTTC 1 cut(s) 789
AsuC2I CCSGG 2 cut(s) 182, 1386
AsuHPI GGTGA 3 cut(s) 263, 524, 1106
AvaI CYCGRG 2 cut(s) 206, 893
AxyI CCTNAGG 2 cut(s) 864, 1011
BalI TGGCCA 1 cut(s) 59
BanII GRGCYC 1 cut(s) 666
BbsI GAAGAC 3 cut(s) 818, 860, 1316
Bbv12I GWGCWC 3 cut(s) 19, 666, 850
BbvCI CCTCAGC 1 cut(s) 165
BbvI GCAGC 5 cut(s) 236, 705, 744, 1193, 1401
BccI CCATC 1 cut(s) 964
BcgI CGANNNNNNTGC 2 cut(s) 827, 861
BciT130I CCWGG 2 cut(s) 723, 1497
BciVI GTATCC 1 cut(s) 616
BclI TGATCA 1 cut(s) 1635
BcnI CCSGG 2 cut(s) 182, 1386
BcoDI GTCTC 4 cut(s) 1181, 1296, 1361, 1441
BfaI CTAG 5 cut(s) 593, 705, 1353, 1469, 1656
BfmI CTRYAG 2 cut(s) 758, 1680
BfrI CTTAAG 1 cut(s) 1393
BfuAI ACCTGC 1 cut(s) 141
BfuI GTATCC 1 cut(s) 616
BglII AGATCT 1 cut(s) 1570
BisI GCNGC 5 cut(s) 225, 719, 758, 1207, 1390
BlsI GCNGC 5 cut(s) 226, 720, 759, 1208, 1391
BmcAI AGTACT 2 cut(s) 1049, 1650
Bme1390I CCNGG 4 cut(s) 182, 723, 1386, 1497
BmeT110I CYCGRG 2 cut(s) 206, 893
BmrFI CCNGG 4 cut(s) 182, 723, 1386, 1497
BmsI GCATC 4 cut(s) 48, 223, 256, 1011
BpiI GAAGAC 3 cut(s) 818, 860, 1316
BplI GAGNNNNNCTC 2 cut(s) 105, 137
Bpu10I CCTNAGC 1 cut(s) 165
BpuEI CTTGAG 8 cut(s) 48, 71, 107, 761, 1133, 1142, 1205, 1271
BpuMI CCSGG 2 cut(s) 182, 1386
BsaHI GRCGYC 1 cut(s) 213
BsaI GGTCTC 1 cut(s) 1181
BsaJI CCNNGG 2 cut(s) 237, 1191
Bsc4I CCNNNNNNNGG 7 cut(s) 509, 800, 865, 1219, 1235, 1376, 1487
Bse21I CCTNAGG 2 cut(s) 864, 1011
Bse3DI GCAATG 3 cut(s) 345, 1004, 1151
BseBI CCWGG 2 cut(s) 723, 1497
BseDI CCNNGG 2 cut(s) 237, 1191
BseGI GGATG 2 cut(s) 39, 583
BseLI CCNNNNNNNGG 7 cut(s) 509, 800, 865, 1219, 1235, 1376, 1487
BseMI GCAATG 3 cut(s) 345, 1004, 1151
BseMII CTCAG 3 cut(s) 156, 878, 1002
BseRI GAGGAG 1 cut(s) 182
BseXI GCAGC 5 cut(s) 236, 705, 744, 1193, 1401
Bsh1236I CGCG 1 cut(s) 837
BshFI GGCC 2 cut(s) 59, 1500
BsiHKAI GWGCWC 3 cut(s) 19, 666, 850
BsiHKCI CYCGRG 2 cut(s) 206, 893
BsiSI CCGG 2 cut(s) 181, 1386
BslFI GGGAC 1 cut(s) 197
BslI CCNNNNNNNGG 7 cut(s) 509, 800, 865, 1219, 1235, 1376, 1487
BsmAI GTCTC 4 cut(s) 1181, 1296, 1361, 1441
BsmFI GGGAC 1 cut(s) 197
BsmI GAATGC 1 cut(s) 1554
BsnI GGCC 2 cut(s) 59, 1500
Bso31I GGTCTC 1 cut(s) 1181
BsoBI CYCGRG 2 cut(s) 206, 893
Bsp1286I GDGCHC 3 cut(s) 19, 666, 850
Bsp143I GATC 6 cut(s) 312, 1001, 1570, 1590, 1635, 1658
BspACI CCGC 2 cut(s) 465, 835
BspANI GGCC 2 cut(s) 59, 1500
BspCNI CTCAG 3 cut(s) 157, 877, 1003
BspFNI CGCG 1 cut(s) 837
BspHI TCATGA 1 cut(s) 1632
BspMAI CTGCAG 1 cut(s) 762
BspMI ACCTGC 1 cut(s) 141
BspPI GGATC 2 cut(s) 1009, 1653
BspTI CTTAAG 1 cut(s) 1393
BspTNI GGTCTC 1 cut(s) 1181
BsrDI GCAATG 3 cut(s) 345, 1004, 1151
BssECI CCNNGG 2 cut(s) 237, 1191
BssMI GATC 6 cut(s) 312, 1001, 1570, 1590, 1635, 1658
BssNI GRCGYC 1 cut(s) 213
BssT1I CCWWGG 1 cut(s) 1191
Bst2UI CCWGG 2 cut(s) 723, 1497
Bst4CI ACNGT 2 cut(s) 1067, 1408
Bst6I CTCTTC 2 cut(s) 411, 1533
BstACI GRCGYC 1 cut(s) 213
BstAFI CTTAAG 1 cut(s) 1393
BstAPI GCANNNNNTGC 1 cut(s) 1381
BstDEI CTNAG 5 cut(s) 165, 309, 639, 864, 1011
BstENI CCTNNNNNAGG 2 cut(s) 863, 1217
BstF5I GGATG 2 cut(s) 39, 583
BstFNI CGCG 1 cut(s) 837
BstKTI GATC 6 cut(s) 315, 1004, 1573, 1593, 1638, 1661
BstMAI GTCTC 4 cut(s) 1181, 1296, 1361, 1441
BstMBI GATC 6 cut(s) 312, 1001, 1570, 1590, 1635, 1658
BstMWI GCNNNNNNNGC 5 cut(s) 118, 233, 266, 661, 1381
BstNI CCWGG 2 cut(s) 723, 1497
BstSCI CCNGG 4 cut(s) 180, 721, 1384, 1495
BstSFI CTRYAG 2 cut(s) 758, 1680
BstUI CGCG 1 cut(s) 837
BstV1I GCAGC 5 cut(s) 236, 705, 744, 1193, 1401
BstV2I GAAGAC 3 cut(s) 818, 860, 1316
BstX2I RGATCY 2 cut(s) 1570, 1658
BstYI RGATCY 2 cut(s) 1570, 1658
Bsu36I CCTNAGG 2 cut(s) 864, 1011
BsuI GTATCC 1 cut(s) 616
BsuRI GGCC 2 cut(s) 59, 1500
BtgZI GCGATG 1 cut(s) 241
BtsCI GGATG 2 cut(s) 39, 583
BtsIMutI CAGTG 1 cut(s) 1448
BveI ACCTGC 1 cut(s) 141
CaiI CAGNNNCTG 2 cut(s) 1381, 1412
CciI TCATGA 1 cut(s) 1632
CseI GACGC 2 cut(s) 812, 1106
Csp6I GTAC 4 cut(s) 674, 1048, 1649, 1670
CviAII CATG 6 cut(s) 146, 161, 293, 524, 1078, 1633
CviQI GTAC 4 cut(s) 674, 1048, 1649, 1670
DdeI CTNAG 5 cut(s) 165, 309, 639, 864, 1011
DpnI GATC 6 cut(s) 314, 1003, 1572, 1592, 1637, 1660
DpnII GATC 6 cut(s) 312, 1001, 1570, 1590, 1635, 1658
DrdI GACNNNNNNGTC 1 cut(s) 191
DseDI GACNNNNNNGTC 1 cut(s) 191
EaeI YGGCCR 1 cut(s) 57
Eam1104I CTCTTC 2 cut(s) 411, 1533
EarI CTCTTC 2 cut(s) 411, 1533
Ecl136II GAGCTC 1 cut(s) 664
Eco130I CCWWGG 1 cut(s) 1191
Eco24I GRGCYC 1 cut(s) 666
Eco31I GGTCTC 1 cut(s) 1181
Eco53kI GAGCTC 1 cut(s) 664
Eco57I CTGAAG 5 cut(s) 839, 872, 1202, 1310, 1523
Eco81I CCTNAGG 2 cut(s) 864, 1011
Eco88I CYCGRG 2 cut(s) 206, 893
EcoICRI GAGCTC 1 cut(s) 664
EcoNI CCTNNNNNAGG 2 cut(s) 863, 1217
EcoRI GAATTC 1 cut(s) 304
EcoRII CCWGG 2 cut(s) 721, 1495
EcoT14I CCWWGG 1 cut(s) 1191
EcoT38I GRGCYC 1 cut(s) 666
ErhI CCWWGG 1 cut(s) 1191
FaeI CATG 6 cut(s) 149, 164, 296, 527, 1081, 1636
FalI AAGNNNNNCTT 2 cut(s) 426, 458
FaqI GGGAC 1 cut(s) 197
FatI CATG 6 cut(s) 145, 160, 292, 523, 1077, 1632
FauNDI CATATG 1 cut(s) 972
FbaI TGATCA 1 cut(s) 1635
Fnu4HI GCNGC 5 cut(s) 225, 719, 758, 1207, 1390
FokI GGATG 2 cut(s) 26, 590
FriOI GRGCYC 1 cut(s) 666
Fsp4HI GCNGC 5 cut(s) 225, 719, 758, 1207, 1390
FspBI CTAG 5 cut(s) 593, 705, 1353, 1469, 1656
GluI GCNGC 5 cut(s) 225, 719, 758, 1207, 1390
HaeIII GGCC 2 cut(s) 59, 1500
HapII CCGG 2 cut(s) 181, 1386
HgaI GACGC 2 cut(s) 812, 1106
Hin1I GRCGYC 1 cut(s) 213
Hin1II CATG 6 cut(s) 149, 164, 296, 527, 1081, 1636
HindIII AAGCTT 2 cut(s) 198, 1424
HpaII CCGG 2 cut(s) 181, 1386
HphI GGTGA 3 cut(s) 263, 524, 1106
Hpy166II GTNNAC 1 cut(s) 728
Hpy188I TCNGA 7 cut(s) 218, 544, 577, 852, 1273, 1329, 1588
Hpy8I GTNNAC 1 cut(s) 728
HpyAV CCTTC 8 cut(s) 314, 544, 620, 869, 930, 938, 1175, 1223
HpyCH4III ACNGT 2 cut(s) 1067, 1408
HpyCH4IV ACGT 2 cut(s) 213, 376
HpyCH4V TGCA 8 cut(s) 149, 224, 425, 752, 760, 879, 990, 1024
HpyF10VI GCNNNNNNNGC 5 cut(s) 118, 233, 266, 661, 1381
HpyF3I CTNAG 5 cut(s) 165, 309, 639, 864, 1011
HpySE526I ACGT 2 cut(s) 213, 376
Hsp92I GRCGYC 1 cut(s) 213
Hsp92II CATG 6 cut(s) 149, 164, 296, 527, 1081, 1636
Ksp22I TGATCA 1 cut(s) 1635
Kzo9I GATC 6 cut(s) 312, 1001, 1570, 1590, 1635, 1658
LmnI GCTCC 2 cut(s) 669, 868
Lsp1109I GCAGC 5 cut(s) 236, 705, 744, 1193, 1401
LweI GCATC 4 cut(s) 48, 223, 256, 1011
MaeI CTAG 5 cut(s) 593, 705, 1353, 1469, 1656
MaeII ACGT 2 cut(s) 213, 376
MaeIII GTNAC 5 cut(s) 251, 1098, 1261, 1408, 1618
MalI GATC 6 cut(s) 314, 1003, 1572, 1592, 1637, 1660
MboI GATC 6 cut(s) 312, 1001, 1570, 1590, 1635, 1658
MflI RGATCY 2 cut(s) 1570, 1658
MhlI GDGCHC 3 cut(s) 19, 666, 850
MlsI TGGCCA 1 cut(s) 59
MluCI AATT 9 cut(s) 96, 304, 558, 886, 977, 993, 1141, 1266, 1301
MluNI TGGCCA 1 cut(s) 59
MlyI GAGTC 5 cut(s) 98, 179, 392, 493, 1033
MmeI TCCRAC 2 cut(s) 568, 1314
Mox20I TGGCCA 1 cut(s) 59
MroXI GAANNNNTTC 1 cut(s) 789
MscI TGGCCA 1 cut(s) 59
MseI TTAA 8 cut(s) 48, 333, 360, 678, 1394, 1428, 1541, 1550
MslI CAYNNNNRTG 1 cut(s) 528
Msp20I TGGCCA 1 cut(s) 59
MspCI CTTAAG 1 cut(s) 1393
MspI CCGG 2 cut(s) 181, 1386
MspR9I CCNGG 4 cut(s) 182, 723, 1386, 1497
Mva1269I GAATGC 1 cut(s) 1554
MvaI CCWGG 2 cut(s) 723, 1497
MvnI CGCG 1 cut(s) 837
MwoI GCNNNNNNNGC 5 cut(s) 118, 233, 266, 661, 1381
NciI CCSGG 2 cut(s) 182, 1386
NdeI CATATG 1 cut(s) 972
NdeII GATC 6 cut(s) 312, 1001, 1570, 1590, 1635, 1658
NlaIII CATG 6 cut(s) 149, 164, 296, 527, 1081, 1636
NmeAIII GCCGAG 1 cut(s) 262
NmuCI GTSAC 2 cut(s) 251, 1261
PaeR7I CTCGAG 2 cut(s) 206, 893
PagI TCATGA 1 cut(s) 1632
PctI GAATGC 1 cut(s) 1554
PdmI GAANNNNTTC 1 cut(s) 789
PflMI CCANNNNNTGG 1 cut(s) 1235
PfoI TCCNGGA 1 cut(s) 180
PkrI GCNGC 5 cut(s) 226, 720, 759, 1208, 1391
PleI GAGTC 5 cut(s) 98, 179, 391, 493, 1033
PpsI GAGTC 5 cut(s) 98, 179, 391, 493, 1033
Psp124BI GAGCTC 1 cut(s) 666
Psp6I CCWGG 2 cut(s) 721, 1495
PspGI CCWGG 2 cut(s) 721, 1495
PspXI VCTCGAGB 1 cut(s) 206
PstI CTGCAG 1 cut(s) 762
PstNI CAGNNNCTG 2 cut(s) 1381, 1412
PsuI RGATCY 2 cut(s) 1570, 1658
RsaI GTAC 4 cut(s) 675, 1049, 1650, 1671
RsaNI GTAC 4 cut(s) 674, 1048, 1649, 1670
RseI CAYNNNNRTG 1 cut(s) 528
SacI GAGCTC 1 cut(s) 666
SaqAI TTAA 8 cut(s) 48, 333, 360, 678, 1394, 1428, 1541, 1550
SatI GCNGC 5 cut(s) 225, 719, 758, 1207, 1390
Sau3AI GATC 6 cut(s) 312, 1001, 1570, 1590, 1635, 1658
ScaI AGTACT 2 cut(s) 1049, 1650
SchI GAGTC 5 cut(s) 98, 179, 392, 493, 1033
ScrFI CCNGG 4 cut(s) 182, 723, 1386, 1497
SduI GDGCHC 3 cut(s) 19, 666, 850
SfaNI GCATC 4 cut(s) 48, 223, 256, 1011
SfcI CTRYAG 2 cut(s) 758, 1680
Sfr274I CTCGAG 2 cut(s) 206, 893
SlaI CTCGAG 2 cut(s) 206, 893
SmiMI CAYNNNNRTG 1 cut(s) 528
Sse9I AATT 9 cut(s) 96, 304, 558, 886, 977, 993, 1141, 1266, 1301
SsiI CCGC 2 cut(s) 465, 835
SspI AATATT 1 cut(s) 683
SspMI CTAG 5 cut(s) 593, 705, 1353, 1469, 1656
SstI GAGCTC 1 cut(s) 666
StyD4I CCNGG 4 cut(s) 180, 721, 1384, 1495
StyI CCWWGG 1 cut(s) 1191
TaaI ACNGT 2 cut(s) 1067, 1408
TaiI ACGT 2 cut(s) 216, 379
TaqI TCGA 5 cut(s) 13, 207, 273, 386, 894
TaqII GACCGA 1 cut(s) 597
TasI AATT 9 cut(s) 96, 304, 558, 886, 977, 993, 1141, 1266, 1301
TatI WGTACW 3 cut(s) 673, 1047, 1648
Tru1I TTAA 8 cut(s) 48, 333, 360, 678, 1394, 1428, 1541, 1550
Tru9I TTAA 8 cut(s) 48, 333, 360, 678, 1394, 1428, 1541, 1550
TscAI CASTG 1 cut(s) 1448
TseFI GTSAC 2 cut(s) 251, 1261
TseI GCWGC 5 cut(s) 224, 718, 757, 1206, 1389
Tsp45I GTSAC 2 cut(s) 251, 1261
TspDTI ATGAA 9 cut(s) 134, 353, 531, 728, 899, 989, 1298, 1427, 1621
TspGWI ACGGA 1 cut(s) 501
TspRI CASTG 1 cut(s) 1448
Van91I CCANNNNNTGG 1 cut(s) 1235
Vha464I CTTAAG 1 cut(s) 1393
XagI CCTNNNNNAGG 2 cut(s) 863, 1217
XapI RAATTY 4 cut(s) 304, 977, 993, 1301
XbaI TCTAGA 1 cut(s) 1468
XhoI CTCGAG 2 cut(s) 206, 893
XmnI GAANNNNTTC 1 cut(s) 789
XspI CTAG 5 cut(s) 593, 705, 1353, 1469, 1656
ZraI GACGTC 1 cut(s) 214
ZrmI AGTACT 2 cut(s) 1049, 1650
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.