Rw1G002890

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0004242
Physical Location & Seq
Forward (+)
5326015 .. 5330312
4298 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G002890.1

Sequence Viewer

Length: 4095 bp
ATGCCTATTGTTCGAGCACTTGAATCTGCTTCTGTAAGCATCCTTCTTAACAAGTTGGCCACTCAAGATGTCATAGATTTCTTCCTCAAGTGGAAAATCGATGATTCCCTGCGAGAAAAGCTCAAGAGAAACCTGCTTGTCATTCATGCAGTTCTCAATCATGCTGAGGAGAAGCAAGTCCGGGACTCAAATGTCAAAGCTTGGCTGGAGGACGTCAGAGTTGCAGCTTATGATGCCGAGGACATATTGGGTGACATCGCCAATGATGCTCTCGAATCACAAAACAGCAAACATGAGGTATGGAATTCTAAGATCAAAGAAGGGATAGACTTTAAGATCAAAGACATTGCTAATACTCTTAATCCCTTTAGGGAACGTGTAGAGTCGAAAATGATAAAGATTATAGATGTATTGGAAGAGATTGCAAAGCAGAAAGATGTTCTTCGTTTGAGAGAAGATTCTGGCGGTATAGAATCAGGTTTTGACGGATTGCCCACGACTCCAATGTTGGGTGATAAATCTCATATTTATGGTAGAGAATCTGATAAGAAGGAGATAATTGCTTTGCTGAAATCGGGTAAAAATGATCGTGAGGTATCTATAATTCCAATTGTAGGCATGGGAGGCATTGGGAAGACTACTCTTGCTCAGCTTGTGTATAATGATGCCAAAGTGAGTAGCTATTTTAATTTGAGAGCATGGGCTTGTGTCTCTGATGTATTTGATGTGCAAAGGATAACAAAAACACTAGTTGAGTCCGCCACAAAATCAAGTTGTAGCACAAACAATTTGGAGTTACTTCAAGAACAGCTGAAAATACGGTTGAAGAACAAGAAATTTCTTTTTGTTCTGGATGATGTCTGGAACGACAAGTATGAAAGCTGGGATAAGTTAAGAGTCCCCTTCACAGTTGGTGCACCAGGGAGTAAAATCATAATCACGACACGAAACAAAACAGTTGCATCACTTATGGGGACTGTTCCTACTTACTGTTTGGAGGGGCTTTCGGACAGTGATTGTTGGTTATTATTCGAACAAATTTTGAAAGTGATTGGCAAGAAAATAGCATACAAGTGTAAAGGGTTGCCTTTGGCTGCAAAGGCACTAGGAGGTCTCCTGCGTGCTGAACCGGAACTAGATGAGAATTACTGGAATGATATCTTGAATAGCAAACTTTGGAAACTGGCAGACAACACTATTCTTCCAGCTCTAAGATTAAGCTACCATCACCTCCCTGGGCATTTGAAGCGCTGCTTTGCTTATTGCTCGATGTTTCCTAAGGACTATGAATATGAGAGAGAGATGTTAATCTTGTTATGGATGGCAGAGGGTTTTGTGCCAGAGCCAGAAGGAAATCAACGGATCGAAGATGTAGCTGGAAGATATGTTTCAGATTTTTTATCAAGGTCCTTCTTCCAACACACCAGTGACAAGTCACGACTGGTAATGCATGACCTCATACATGATCTTGCACAATCTGTTTCTGGCAAAACATTTGTTAGGTTGGAGGAAAATTCAGAGAACCATAAAAAGTCTACAAAGGCTCGGCACTTGTCATATACCCGTGGATCTGAAGATGTATTTCAGAAATTTGAGGCATTCAGTGAGGTGGATTGTTTACGAACCTTTCTACCGCTAGACCCGTTGCAAGGATTCAATGTGAGTGGCTTATCTGACAAGGTTCCTTGTGATATGATGCCAAAATTAAGACTCCTGCGGGTGCTATCCTTTAGTGGTTATCTCATAGAAAAGTTGCCAGATTCAATTGGCAACTTGAAGCATCTGCGATACCTCAATCTTTCTTATTGTCAAATAGAAGAGCTACCTGAGCCAACAAGCAGTCTATACAATCTGCAGACATTAATTTTATTCAGATGTCGATCGCTTACCATGCTGCCTGCAGACATAGGAAACCTAAGGAATTTACGGCATCTCAATATTCAGGGAACTAGGCTGAAAAGTATGCCTTTGGGAATGGGAAGATTGGTTAACCTTCAAATACTGTCAGATTTTATTGTGGGGAAAGCAATGGGAAAAGGGATTGCAGAATTGAAGGACTTGTCACATCTTCGAGGCTCAGTTTCTATTTTGTGTTTGCATGATGTCAGCAGTGTTAGGGATGCAATTGAGGCCAGATTAGATGTTAAGATGCACCTTGATGATTTAGTTTTGGAATGGAGCAGCAGTACTGATGGTACAGAATTTCCATATTGGATGGGGAATCCTTTGTTCACTAACATGGCGTACTTGCATCTCTATGGTTGCATAAAATGCACATCTTTACCACCCCTTGGCCAACTACCTTCTCTTAAAGATCTCATAATTGAAGCAATGCATGGAATACAGTGTGTAGGAATGGATTTTTTTGGGGATGATGATGGTTCCACAATTTTTTTCCCCTATTTAGAGACTTTGAAATTTGAGGACATGAAAGAATGGGAGAAATGGTCATCTTCAGAAGATGAAAGGCTTAAACGATTTCCCAGCCTTCGGGAGCTGTCCATATTTAGATGTCCCAAGTTAACAAAGTTTACACATGGATTTGCTTCTCTAGAAAAGCTGCGCATCAAGAACTGCGGAGCAATAACTACCTTCTCACAGAATCCTGCACTTGGGAATTTGGAACCTGTAGAATTCCCTAGCCTGAATTCGGAACCTGTAGAATTCCCTAGCCTGCAACTGCTTGTTCTTGTTGGTTGCAGTGAGCTTGAAAACCTTCCTTTTTCCCTCCCTTTGTTGAAAATGCTTGAGATAGATGGTTGTGAAAAATTGGCTGCACTTCCGAGGATGATGGAATTTAGCTCGCTGACCTCTTTGCACCTAACTCATATTCCAGATGTGAAGCATCTACGTGAAGGCTTTATCCAACGGTCTGCAAAACTTGAAAAATTGAGTCTTACAAGCTTTCGTGACCTTGAATATTTGGCAGATGACCGAGTTGGACTAGCACACCTTGTATCCCTTCAACGCTTGACAATATCCCATTGTCCTGAGTTTGTTGCTTTGCCTGATGAGCTCCCACCTGTACTTAAATATTTGGATTTGAAACATTGTCCTAGCCTGATGAAGCTGCCTGGTAAACTTCACAAGCTTGAGTGTCTTGCAGAGCTGCAGATTGAATGGTGTTCAAAGATTGAAGCATTTCCAGATGTGGGGTTGCCTGACAAACTGAAGCGTCTTGTAATCCGCGAATGTGGTGCTCTGAAGACCCTTCCTCAGGAGCTTCTCTGCAATAACAATTTTCTCGAGTATTTAGAAGTTCATAAATGTCCTTCTCTTGTGTCAGTTCTTGAAGAAGGAAACTTGCCATCAACTCTAACACATATGAAATTTTACTATTGCAAAATTCTGGGATCACTGCCTGAGGGTTTGATGCACAAAGACAATATGACTCTAAAGTACTTGGAGATAGACAACTGTTCCTCTCTCAAGTCCTTTCCAAATGGTGAGTTACCAACAAGCCTTGAGCGTCTTGAGATTAGTGAATGCTCTAATTTCCAGACATTGCCATTGAGCCTTCTCAACCTCACGAATCTTGAGACCTTGGAAGTAAAGAGCTGCCCCCTTCTTCAGGACTTTCCAGAGAATCTCAAGTCTCTACCTAAATTCTTTCATAAACTCAAATGTCTTCAGAAGTTGGAGATTTCTGGCTGTCCTAGTCTCCTGTCCTTGCCAAAGCAGGGTCTGCCCAGCAGCTTAAGACTACTTACAGTTACTGATTGTGAAAAGCTTAATCCCATACATCAGTGGAGACTTCACAAACTACCCTCTCTAGATGTTTTCACCATTGGTGGATTTCCTGGCCTGAAGTCCTTTTCGAAAGATTATCCTCTTCCTCACACTTTAACCTCTTTAAGCATTCAAAGATTCCCAGATCTTGAATCCATATCGGAGGTGCTTGAGAATCTCACCTCACTTGAAAACCTGAGTGTCAGGGAGTGTGATAAGCTCCAATCTTTGCCAGAGACGGGGCTACCAGCGACAATTTCTACCTTGACAATTCGGAATTGTTTACTTCTAGAACCTCGATGTGAAAAGGACAAAGGGGAAGATTGGTCCATTATCAAAAACATCCCCTTTGTGAGTTCACGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

1364

Amino Acids

154.51

Weight (kDa)

6.2

Isoelectric Point (pI)

46.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 12 - 98 2.2e-15 Rx N-terminal domain
NB-ARC PF00931 181 - 347 1.8e-33 NB-ARC domain
WHD_DRP PF23559 424 - 491 1e-24 Disease resistance protein Winged helix domain
LRR_14 PF23598 566 - 776 1.7e-22 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 681 - 730 1.4e-08 R13L1/DRL21 LRRs
LRR_R13L1-DRL21 PF25019 731 - 778 7.4e-11 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 191
AatII GACGTC 1 cut(s) 216
Acc16I TGCGCA 1 cut(s) 2564
Acc36I ACCTGC 1 cut(s) 141
AccB7I CCANNNNNTGG 1 cut(s) 2291
AccI GTMKAC 1 cut(s) 1535
AccII CGCG 1 cut(s) 3189
AciI CCGC 6 cut(s) 465, 759, 1634, 1717, 2577, 3187
AclWI GGATC 3 cut(s) 1370, 1576, 3361
AcoI YGGCCR 2 cut(s) 57, 2293
AcuI CTGAAG 7 cut(s) 1593, 2439, 3191, 3224, 3554, 3614, 3827
AcyI GRCGYC 1 cut(s) 213
AfaI GTAC 5 cut(s) 2188, 2197, 2246, 3027, 3401
AfeI AGCGCT 1 cut(s) 1250
AflII CTTAAG 1 cut(s) 3697
AflIII ACRYGT 1 cut(s) 376
AhlI ACTAGT 1 cut(s) 748
AjnI CCWGG 4 cut(s) 919, 1234, 3073, 3799
AleI CACNNNNGTG 1 cut(s) 1425
Alw21I GWGCWC 4 cut(s) 19, 919, 3018, 3202
Alw26I GTCTC 8 cut(s) 715, 1118, 2402, 3533, 3600, 3665, 3745, 3959
Alw44I GTGCAC 1 cut(s) 915
AlwI GGATC 3 cut(s) 1370, 1576, 3361
AlwNI CAGNNNCTG 2 cut(s) 3685, 3716
Ama87I CYCGRG 1 cut(s) 3245
Aor51HI AGCGCT 1 cut(s) 1250
AoxI GGCC 4 cut(s) 57, 2130, 2293, 3802
ApaLI GTGCAC 1 cut(s) 915
AseI ATTAAT 1 cut(s) 1862
Asp700I GAANNNNTTC 2 cut(s) 2715, 3141
AspLEI GCGC 2 cut(s) 1251, 2565
AspS9I GGNCC 2 cut(s) 1407, 4056
AsuC2I CCSGG 1 cut(s) 182
AsuHPI GGTGA 6 cut(s) 263, 524, 1220, 3458, 3775, 3901
AsuII TTCGAA 2 cut(s) 1032, 3818
AvaI CYCGRG 1 cut(s) 3245
AvaII GGWCC 2 cut(s) 1407, 4056
AxyI CCTNAGG 4 cut(s) 1278, 1916, 3216, 3363
BaeGI GKGCMC 1 cut(s) 919
BalI TGGCCA 2 cut(s) 59, 2295
BanII GRGCYC 1 cut(s) 3018
BarI GAAGNNNNNNTAC 2 cut(s) 27, 59
BbsI GAAGAC 3 cut(s) 641, 3212, 3620
Bbv12I GWGCWC 4 cut(s) 19, 919, 3018, 3202
BbvCI CCTCAGC 1 cut(s) 165
BccI CCATC 8 cut(s) 1233, 1315, 2186, 2209, 2372, 2750, 2785, 3316
BceAI ACGGC 1 cut(s) 1943
BcgI CGANNNNNNTGC 2 cut(s) 3179, 3213
BciT130I CCWGG 4 cut(s) 921, 1236, 3075, 3801
BciVI GTATCC 1 cut(s) 2968
BcnI CCSGG 1 cut(s) 182
BcoDI GTCTC 8 cut(s) 715, 1118, 2402, 3533, 3600, 3665, 3745, 3959
BcuI ACTAGT 1 cut(s) 748
BfmI CTRYAG 5 cut(s) 1853, 1899, 2628, 2658, 3110
BfoI RGCGCY 1 cut(s) 1252
BfrI CTTAAG 1 cut(s) 3697
BfuAI ACCTGC 1 cut(s) 141
BfuI GTATCC 1 cut(s) 2968
BglII AGATCT 2 cut(s) 2314, 3874
BlpI GCTNAGC 1 cut(s) 648
BmcAI AGTACT 2 cut(s) 2188, 3401
Bme1390I CCNGG 5 cut(s) 182, 921, 1236, 3075, 3801
Bme18I GGWCC 2 cut(s) 1407, 4056
BmeT110I CYCGRG 1 cut(s) 3245
BmgT120I GGNCC 2 cut(s) 1407, 4056
BmiI GGNNCC 4 cut(s) 1683, 2383, 2625, 2655
BmrFI CCNGG 5 cut(s) 182, 921, 1236, 3075, 3801
BpiI GAAGAC 3 cut(s) 641, 3212, 3620
BplI GAGNNNNNCTC 2 cut(s) 105, 137
BpmI CTGGAG 1 cut(s) 227
Bpu10I CCTNAGC 2 cut(s) 165, 1827
Bpu1102I GCTNAGC 1 cut(s) 648
Bpu14I TTCGAA 2 cut(s) 1032, 3818
BpuMI CCSGG 1 cut(s) 182
Bsa29I ATCGAT 1 cut(s) 99
BsaAI YACGTR 1 cut(s) 2852
BsaBI GATNNNNATC 2 cut(s) 1307, 1879
BsaHI GRCGYC 1 cut(s) 213
BsaI GGTCTC 2 cut(s) 1118, 3533
BsaJI CCNNGG 8 cut(s) 237, 920, 1234, 1235, 1564, 2290, 2783, 3543
BsaWI WCCGGW 1 cut(s) 1129
BsaXI ACNNNNNCTCC 4 cut(s) 2170, 2200, 2432, 2462
Bse1I ACTGG 4 cut(s) 1154, 1188, 1425, 1446
Bse21I CCTNAGG 4 cut(s) 1278, 1916, 3216, 3363
Bse3DI GCAATG 4 cut(s) 345, 2034, 2337, 3503
Bse8I GATNNNNATC 2 cut(s) 1307, 1879
BseBI CCWGG 4 cut(s) 921, 1236, 3075, 3801
BseCI ATCGAT 1 cut(s) 99
BseDI CCNNGG 8 cut(s) 237, 920, 1234, 1235, 1564, 2290, 2783, 3543
BseGI GGATG 8 cut(s) 39, 859, 1326, 2125, 2220, 2377, 2793, 4071
BseJI GATNNNNATC 2 cut(s) 1307, 1879
BseMI GCAATG 4 cut(s) 345, 2034, 2337, 3503
BseMII CTCAG 8 cut(s) 156, 662, 1818, 2091, 2982, 3230, 3354, 3917
BseNI ACTGG 4 cut(s) 1154, 1188, 1425, 1446
BseRI GAGGAG 1 cut(s) 182
BseSI GKGCMC 1 cut(s) 919
BseYI CCCAGC 3 cut(s) 882, 2483, 3689
BsgI GTGCAG 2 cut(s) 2592, 2760
Bsh1236I CGCG 1 cut(s) 3189
Bsh1285I CGRYCG 1 cut(s) 1883
BshFI GGCC 4 cut(s) 59, 2132, 2295, 3804
BshVI ATCGAT 1 cut(s) 99
BsiEI CGRYCG 1 cut(s) 1883
BsiHKAI GWGCWC 4 cut(s) 19, 919, 3018, 3202
BsiHKCI CYCGRG 1 cut(s) 3245
BsiSI CCGG 2 cut(s) 181, 1130
BslFI GGGAC 4 cut(s) 197, 884, 988, 2499
BsmAI GTCTC 8 cut(s) 715, 1118, 2402, 3533, 3600, 3665, 3745, 3959
BsmBI CGTCTC 1 cut(s) 3959
BsmFI GGGAC 4 cut(s) 197, 884, 988, 2499
BsmI GAATGC 3 cut(s) 1598, 3491, 3858
BsnI GGCC 4 cut(s) 59, 2132, 2295, 3804
Bso31I GGTCTC 2 cut(s) 1118, 3533
BsoBI CYCGRG 1 cut(s) 3245
Bsp119I TTCGAA 2 cut(s) 1032, 3818
Bsp1286I GDGCHC 4 cut(s) 19, 919, 3018, 3202
Bsp1720I GCTNAGC 1 cut(s) 648
BspACI CCGC 6 cut(s) 465, 759, 1634, 1717, 2577, 3187
BspANI GGCC 4 cut(s) 59, 2132, 2295, 3804
BspCNI CTCAG 8 cut(s) 157, 661, 1819, 2090, 2983, 3229, 3355, 3918
BspDI ATCGAT 1 cut(s) 99
BspFNI CGCG 1 cut(s) 3189
BspLI GGNNCC 4 cut(s) 1683, 2383, 2625, 2655
BspMAI CTGCAG 3 cut(s) 1857, 1903, 3114
BspMI ACCTGC 1 cut(s) 141
BspPI GGATC 3 cut(s) 1370, 1576, 3361
BspQI GCTCTTC 1 cut(s) 1812
BspT104I TTCGAA 2 cut(s) 1032, 3818
BspTI CTTAAG 1 cut(s) 3697
BspTNI GGTCTC 2 cut(s) 1118, 3533
BsrDI GCAATG 4 cut(s) 345, 2034, 2337, 3503
BsrI ACTGG 4 cut(s) 1154, 1188, 1425, 1446
BssECI CCNNGG 8 cut(s) 237, 920, 1234, 1235, 1564, 2290, 2783, 3543
BssNI GRCGYC 1 cut(s) 213
BssT1I CCWWGG 2 cut(s) 2290, 3543
Bst2UI CCWGG 4 cut(s) 921, 1236, 3075, 3801
Bst6I CTCTTC 3 cut(s) 411, 1812, 3837
BstACI GRCGYC 1 cut(s) 213
BstAFI CTTAAG 1 cut(s) 3697
BstAPI GCANNNNNTGC 2 cut(s) 2271, 3685
BstBAI YACGTR 1 cut(s) 2852
BstBI TTCGAA 2 cut(s) 1032, 3818
BstC8I GCNNGC 4 cut(s) 1122, 1899, 2675, 2804
BstDSI CCRYGG 1 cut(s) 1564
BstENI CCTNNNNNAGG 2 cut(s) 3215, 3569
BstF5I GGATG 8 cut(s) 39, 859, 1326, 2125, 2220, 2377, 2793, 4071
BstFNI CGCG 1 cut(s) 3189
BstH2I RGCGCY 1 cut(s) 1252
BstHHI GCGC 2 cut(s) 1251, 2565
BstMAI GTCTC 8 cut(s) 715, 1118, 2402, 3533, 3600, 3665, 3745, 3959
BstMCI CGRYCG 1 cut(s) 1883
BstNI CCWGG 4 cut(s) 921, 1236, 3075, 3801
BstSCI CCNGG 5 cut(s) 180, 919, 1234, 3073, 3799
BstSFI CTRYAG 5 cut(s) 1853, 1899, 2628, 2658, 3110
BstSLI GKGCMC 1 cut(s) 919
BstUI CGCG 1 cut(s) 3189
BstV2I GAAGAC 3 cut(s) 641, 3212, 3620
BstX2I RGATCY 3 cut(s) 1568, 2314, 3874
BstYI RGATCY 3 cut(s) 1568, 2314, 3874
Bsu15I ATCGAT 1 cut(s) 99
Bsu36I CCTNAGG 4 cut(s) 1278, 1916, 3216, 3363
BsuI GTATCC 1 cut(s) 2968
BsuRI GGCC 4 cut(s) 59, 2132, 2295, 3804
BsuTUI ATCGAT 1 cut(s) 99
BtgI CCRYGG 1 cut(s) 1564
BtgZI GCGATG 1 cut(s) 241
BtsCI GGATG 8 cut(s) 39, 859, 1326, 2125, 2220, 2377, 2793, 4071
BtsI GCAGTG 3 cut(s) 2116, 2707, 3356
BtsIMutI CAGTG 8 cut(s) 1018, 1432, 1609, 2116, 2351, 2707, 3356, 3752
BveI ACCTGC 1 cut(s) 141
Cac8I GCNNGC 4 cut(s) 1122, 1899, 2675, 2804
CaiI CAGNNNCTG 2 cut(s) 3685, 3716
CfoI GCGC 2 cut(s) 1251, 2565
Cfr13I GGNCC 2 cut(s) 1407, 4056
ClaI ATCGAT 1 cut(s) 99
CseI GACGC 2 cut(s) 3164, 3458
Csp6I GTAC 5 cut(s) 2187, 2196, 2245, 3026, 3400
CspCI CAANNNNNGTGG 2 cut(s) 1645, 1680
CviQI GTAC 5 cut(s) 2187, 2196, 2245, 3026, 3400
DrdI GACNNNNNNGTC 1 cut(s) 191
DseDI GACNNNNNNGTC 1 cut(s) 191
EaeI YGGCCR 2 cut(s) 57, 2293
Eam1104I CTCTTC 3 cut(s) 411, 1812, 3837
EarI CTCTTC 3 cut(s) 411, 1812, 3837
EciI GGCGGA 1 cut(s) 748
Ecl136II GAGCTC 1 cut(s) 3016
Eco130I CCWWGG 2 cut(s) 2290, 3543
Eco24I GRGCYC 1 cut(s) 3018
Eco31I GGTCTC 2 cut(s) 1118, 3533
Eco32I GATATC 1 cut(s) 1159
Eco47I GGWCC 2 cut(s) 1407, 4056
Eco47III AGCGCT 1 cut(s) 1250
Eco53kI GAGCTC 1 cut(s) 3016
Eco57I CTGAAG 7 cut(s) 1593, 2439, 3191, 3224, 3554, 3614, 3827
Eco81I CCTNAGG 4 cut(s) 1278, 1916, 3216, 3363
Eco88I CYCGRG 1 cut(s) 3245
EcoICRI GAGCTC 1 cut(s) 3016
EcoNI CCTNNNNNAGG 2 cut(s) 3215, 3569
EcoO109I RGGNCCY 1 cut(s) 1407
EcoRI GAATTC 4 cut(s) 304, 2633, 2647, 2663
EcoRII CCWGG 4 cut(s) 919, 1234, 3073, 3799
EcoRV GATATC 1 cut(s) 1159
EcoT14I CCWWGG 2 cut(s) 2290, 3543
EcoT22I ATGCAT 2 cut(s) 1452, 2337
EcoT38I GRGCYC 1 cut(s) 3018
ErhI CCWWGG 2 cut(s) 2290, 3543
Esp3I CGTCTC 1 cut(s) 3959
FalI AAGNNNNNCTT 6 cut(s) 426, 458, 1238, 1270, 1951, 1983
FaqI GGGAC 4 cut(s) 197, 884, 988, 2499
FauI CCCGC 1 cut(s) 1710
FauNDI CATATG 1 cut(s) 3324
FblI GTMKAC 1 cut(s) 1535
FokI GGATG 8 cut(s) 26, 866, 1333, 2132, 2227, 2384, 2800, 4058
FriOI GRGCYC 1 cut(s) 3018
FspI TGCGCA 1 cut(s) 2564
GlaI GCGC 2 cut(s) 1250, 2564
GsaI CCCAGC 3 cut(s) 886, 2487, 3693
GsuI CTGGAG 1 cut(s) 227
HaeII RGCGCY 1 cut(s) 1252
HaeIII GGCC 4 cut(s) 59, 2132, 2295, 3804
HapII CCGG 2 cut(s) 181, 1130
HgaI GACGC 2 cut(s) 3164, 3458
HhaI GCGC 2 cut(s) 1251, 2565
Hin1I GRCGYC 1 cut(s) 213
Hin6I GCGC 2 cut(s) 1249, 2563
HinP1I GCGC 2 cut(s) 1249, 2563
HincII GTYRAC 2 cut(s) 1990, 2523
HindII GTYRAC 2 cut(s) 1990, 2523
HindIII AAGCTT 4 cut(s) 198, 2902, 3089, 3728
HpaI GTTAAC 2 cut(s) 1990, 2523
HpaII CCGG 2 cut(s) 181, 1130
HphI GGTGA 6 cut(s) 263, 524, 1220, 3458, 3775, 3901
HpyCH4IV ACGT 3 cut(s) 213, 376, 2851
HpySE526I ACGT 3 cut(s) 213, 376, 2851
Hsp92I GRCGYC 1 cut(s) 213
HspAI GCGC 2 cut(s) 1249, 2563
KspAI GTTAAC 2 cut(s) 1990, 2523
LguI GCTCTTC 1 cut(s) 1812
LmnI GCTCC 6 cut(s) 2178, 2494, 2579, 3021, 3220, 3954
MaeII ACGT 3 cut(s) 213, 376, 2851
MaeIII GTNAC 8 cut(s) 251, 795, 1427, 1434, 2061, 2909, 3450, 3712
MfeI CAATTG 3 cut(s) 609, 1764, 2124
MflI RGATCY 3 cut(s) 1568, 2314, 3874
MhlI GDGCHC 4 cut(s) 19, 919, 3018, 3202
MlsI TGGCCA 2 cut(s) 59, 2295
MluNI TGGCCA 2 cut(s) 59, 2295
MlyI GAGTC 8 cut(s) 179, 392, 493, 764, 906, 1704, 2902, 3385
MmeI TCCRAC 5 cut(s) 1441, 1485, 2890, 2920, 3618
Mox20I TGGCCA 2 cut(s) 59, 2295
Mph1103I ATGCAT 2 cut(s) 1452, 2337
MroXI GAANNNNTTC 2 cut(s) 2715, 3141
MscI TGGCCA 2 cut(s) 59, 2295
MslI CAYNNNNRTG 7 cut(s) 528, 1072, 1425, 2157, 2237, 2256, 2850
Msp20I TGGCCA 2 cut(s) 59, 2295
MspA1I CMGCKG 1 cut(s) 811
MspCI CTTAAG 1 cut(s) 3697
MspI CCGG 2 cut(s) 181, 1130
MspR9I CCNGG 5 cut(s) 182, 921, 1236, 3075, 3801
MunI CAATTG 3 cut(s) 609, 1764, 2124
Mva1269I GAATGC 3 cut(s) 1598, 3491, 3858
MvaI CCWGG 4 cut(s) 921, 1236, 3075, 3801
MvnI CGCG 1 cut(s) 3189
NciI CCSGG 1 cut(s) 182
NdeI CATATG 1 cut(s) 3324
NlaIV GGNNCC 4 cut(s) 1683, 2383, 2625, 2655
NmeAIII GCCGAG 2 cut(s) 262, 1525
NmuCI GTSAC 5 cut(s) 251, 1427, 1434, 2061, 2909
NsbI TGCGCA 1 cut(s) 2564
NsiI ATGCAT 2 cut(s) 1452, 2337
NspV TTCGAA 2 cut(s) 1032, 3818
OliI CACNNNNGTG 1 cut(s) 1425
PaeR7I CTCGAG 1 cut(s) 3245
PasI CCCWGGG 1 cut(s) 1235
PciSI GCTCTTC 1 cut(s) 1812
PctI GAATGC 3 cut(s) 1598, 3491, 3858
PdmI GAANNNNTTC 2 cut(s) 2715, 3141
PflMI CCANNNNNTGG 1 cut(s) 2291
PfoI TCCNGGA 1 cut(s) 180
Ple19I CGATCG 1 cut(s) 1883
PleI GAGTC 8 cut(s) 179, 391, 493, 763, 905, 1704, 2901, 3385
PpsI GAGTC 8 cut(s) 179, 391, 493, 763, 905, 1704, 2901, 3385
Ppu21I YACGTR 1 cut(s) 2852
PpuMI RGGWCCY 1 cut(s) 1407
PshBI ATTAAT 1 cut(s) 1862
Psp124BI GAGCTC 1 cut(s) 3018
Psp5II RGGWCCY 1 cut(s) 1407
Psp6I CCWGG 4 cut(s) 919, 1234, 3073, 3799
PspFI CCCAGC 3 cut(s) 882, 2483, 3689
PspGI CCWGG 4 cut(s) 919, 1234, 3073, 3799
PspN4I GGNNCC 4 cut(s) 1683, 2383, 2625, 2655
PspPI GGNCC 2 cut(s) 1407, 4056
PspPPI RGGWCCY 1 cut(s) 1407
PstI CTGCAG 3 cut(s) 1857, 1903, 3114
PstNI CAGNNNCTG 2 cut(s) 3685, 3716
PsuI RGATCY 3 cut(s) 1568, 2314, 3874
PvuI CGATCG 1 cut(s) 1883
PvuII CAGCTG 1 cut(s) 811
RsaI GTAC 5 cut(s) 2188, 2197, 2246, 3027, 3401
RsaNI GTAC 5 cut(s) 2187, 2196, 2245, 3026, 3400
RseI CAYNNNNRTG 7 cut(s) 528, 1072, 1425, 2157, 2237, 2256, 2850
SacI GAGCTC 1 cut(s) 3018
SapI GCTCTTC 1 cut(s) 1812
Sau96I GGNCC 2 cut(s) 1407, 4056
ScaI AGTACT 2 cut(s) 2188, 3401
SchI GAGTC 8 cut(s) 179, 392, 493, 764, 906, 1704, 2902, 3385
ScrFI CCNGG 5 cut(s) 182, 921, 1236, 3075, 3801
SduI GDGCHC 4 cut(s) 19, 919, 3018, 3202
SfcI CTRYAG 5 cut(s) 1853, 1899, 2628, 2658, 3110
Sfr274I CTCGAG 1 cut(s) 3245
SfuI TTCGAA 2 cut(s) 1032, 3818
SinI GGWCC 2 cut(s) 1407, 4056
SlaI CTCGAG 1 cut(s) 3245
SmiMI CAYNNNNRTG 7 cut(s) 528, 1072, 1425, 2157, 2237, 2256, 2850
SpeI ACTAGT 1 cut(s) 748
SsiI CCGC 6 cut(s) 465, 759, 1634, 1717, 2577, 3187
SspI AATATT 3 cut(s) 1939, 2921, 3035
SstI GAGCTC 1 cut(s) 3018
StyD4I CCNGG 5 cut(s) 180, 919, 1234, 3073, 3799
StyI CCWWGG 2 cut(s) 2290, 3543
TaiI ACGT 3 cut(s) 216, 379, 2854
TaqII GACCGA 1 cut(s) 2949
TatI WGTACW 3 cut(s) 2186, 3025, 3399
TscAI CASTG 8 cut(s) 1018, 1432, 1609, 2116, 2351, 2707, 3363, 3752
TseFI GTSAC 5 cut(s) 251, 1427, 1434, 2061, 2909
Tsp45I GTSAC 5 cut(s) 251, 1427, 1434, 2061, 2909
TspDTI ATGAA 9 cut(s) 134, 891, 1302, 2444, 2478, 3080, 3251, 3341, 3602
TspGWI ACGGA 2 cut(s) 501, 1375
TspRI CASTG 8 cut(s) 1018, 1432, 1609, 2116, 2351, 2707, 3363, 3752
Van91I CCANNNNNTGG 1 cut(s) 2291
Vha464I CTTAAG 1 cut(s) 3697
VneI GTGCAC 1 cut(s) 915
VpaK11BI GGWCC 2 cut(s) 1407, 4056
VspI ATTAAT 1 cut(s) 1862
XagI CCTNNNNNAGG 2 cut(s) 3215, 3569
XbaI TCTAGA 3 cut(s) 2551, 3772, 4018
XcmI CCANNNNNNNNNTGG 1 cut(s) 1232
XhoI CTCGAG 1 cut(s) 3245
XmiI GTMKAC 1 cut(s) 1535
XmnI GAANNNNTTC 2 cut(s) 2715, 3141
ZraI GACGTC 1 cut(s) 214
ZrmI AGTACT 2 cut(s) 2188, 3401
Zsp2I ATGCAT 2 cut(s) 1452, 2337
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.