Rorug01G0025600

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0004242
Physical Location & Seq
Forward (+)
4276507 .. 4279506
3000 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0025600.1

Sequence Viewer

Length: 978 bp
ATGAAGGCGGTAGTGGTAACGACCCCCGGCGGACCGGAGGTCCTCCGAGTACAAGAAGTAGAAGACCCAGAAATCAAGGACGACGAGGTTCTTATCAGAGTGGAGGCCACCGCGCTGAACGGAGCCGACACTTTTCAGAGGAAGGGACTGTACCCACCACCTCCGGGGTCCAGTCACTACCTGGGCGTCGAATGTTCTGGAACCATCGAGGCCGTCGGGAAACACGTGTCAAGATGGAAAGTGGGTGATCAGGTGTGTGCTTTACTTAATGGAGGAGGGTATGCTGAGAAGGTGGCTGTTCCGGCGGGACAGGTTCTACCGGTTCCACCTGGGATTTCTTTGAAGGATGCTGCGAGCTTTCCGGAGGTGGCGTGCACTGTCTGGTCCACCATTTTTATGATGAGCCGGCTATCTTCGGGCGAAACACTTTTGGTTCATGGGGGTTCCAGCGGAATCGGTACATTTGCTATTCAGATTGCTAAATACTGGGGAGTAAGGGTATTTGTCACAGCAGGTAGTGAGGAAAAGTTAGCAGCTTGTAAGGAGCTTGGGGCTGACGTAGGTATCAATTACAAGACGGAGGACTTTGTTACACGGGTAAAGGAAGAGACAGGCGGCAAAGGTGTTGAAGTCATCTTGGATAGCATTGGGCAAGCCTATTTTAAATGTAACCTTGACAGCTTAGCTTTTGATGGGAGGCTTTTTGTCATTGGGACACTGAGTGGCGCTGTCTCAGAAATAGATCTTCGTATGGTGCTTTCAAAGCGCCTCACCATCCAAGCGGCTGGTTTGCGATTCAGAAGTCCAGAAAACAAAGCAGTAATTGTTAGAGAGGTGGAGGAGAACGTGTGGCCTGCAATTGTGGCTGGCAAGGTGAAAACTGTTGTCTACAAGCGTTTCCCATTATCTGAAGCCGCTGAGGCTCACCAGCTATTGGAAACTAGCCAGCATATTGGAAAGATACTACTTTTTCCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

325

Amino Acids

34.84

Weight (kDa)

5.85

Isoelectric Point (pI)

44.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N PF08240 27 - 109 2.3e-18 Alcohol dehydrogenase GroES-like domain
ADH_zinc_N PF00107 151 - 263 1.9e-30 Zinc-binding dehydrogenase
ADH_zinc_N_2 PF13602 183 - 323 1.7e-22 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 503
AccB7I CCANNNNNTGG 1 cut(s) 934
AccI GTMKAC 1 cut(s) 888
AccII CGCG 1 cut(s) 113
AccIII TCCGGA 1 cut(s) 361
AciI CCGC 8 cut(s) 8, 30, 111, 305, 450, 615, 782, 915
AcuI CTGAAG 1 cut(s) 930
AcvI CACGTG 1 cut(s) 226
AcyI GRCGYC 1 cut(s) 186
AdeI CACNNNGTG 1 cut(s) 722
AfaI GTAC 3 cut(s) 51, 152, 460
AfiI CCNNNNNNNGG 2 cut(s) 164, 934
AflIII ACRYGT 3 cut(s) 223, 225, 846
AgeI ACCGGT 1 cut(s) 319
AgsI TTSAA 3 cut(s) 343, 629, 762
AhdI GACNNNNNGTC 1 cut(s) 38
AjnI CCWGG 2 cut(s) 180, 328
AluBI AGCT 6 cut(s) 357, 536, 547, 681, 686, 931
AluI AGCT 6 cut(s) 357, 536, 547, 681, 686, 931
Alw21I GWGCWC 1 cut(s) 377
Alw26I GTCTC 2 cut(s) 602, 736
Alw44I GTGCAC 1 cut(s) 373
Aor13HI TCCGGA 1 cut(s) 361
AoxI GGCC 3 cut(s) 105, 210, 851
ApaLI GTGCAC 1 cut(s) 373
ApeKI GCWGC 2 cut(s) 350, 533
AsiGI ACCGGT 1 cut(s) 319
AspLEI GCGC 3 cut(s) 115, 728, 768
AspS9I GGNCC 4 cut(s) 32, 40, 168, 384
AsuC2I CCSGG 2 cut(s) 27, 165
AsuHPI GGTGA 4 cut(s) 257, 763, 886, 917
AvaII GGWCC 4 cut(s) 32, 40, 168, 384
BaeGI GKGCMC 1 cut(s) 377
BaeI ACNNNNGTAYC 2 cut(s) 547, 580
BarI GAAGNNNNNNTAC 2 cut(s) 134, 166
BbrPI CACGTG 1 cut(s) 226
BbsI GAAGAC 1 cut(s) 69
Bbv12I GWGCWC 1 cut(s) 377
BbvCI CCTCAGC 1 cut(s) 918
BbvI GCAGC 2 cut(s) 337, 545
BccI CCATC 4 cut(s) 212, 228, 686, 782
BceAI ACGGC 1 cut(s) 197
BciT130I CCWGG 2 cut(s) 182, 330
BclI TGATCA 1 cut(s) 247
BcnI CCSGG 2 cut(s) 27, 165
BcoDI GTCTC 2 cut(s) 602, 736
BfaI CTAG 1 cut(s) 942
BfoI RGCGCY 2 cut(s) 729, 769
BfuAI ACCTGC 1 cut(s) 503
BglI GCCNNNNNGGC 1 cut(s) 920
BglII AGATCT 1 cut(s) 742
BisI GCNGC 5 cut(s) 351, 534, 616, 783, 915
BlpI GCTNAGC 1 cut(s) 682
BlsI GCNGC 5 cut(s) 352, 535, 617, 784, 916
Bme1390I CCNGG 4 cut(s) 27, 165, 182, 330
Bme18I GGWCC 4 cut(s) 32, 40, 168, 384
BmeRI GACNNNNNGTC 1 cut(s) 38
BmgT120I GGNCC 4 cut(s) 32, 40, 168, 384
BmiI GGNNCC 5 cut(s) 124, 169, 202, 324, 445
BmrFI CCNGG 4 cut(s) 27, 165, 182, 330
BmrI ACTGGG 1 cut(s) 496
BmsI GCATC 1 cut(s) 337
BmuI ACTGGG 1 cut(s) 496
BpiI GAAGAC 1 cut(s) 69
Bpu10I CCTNAGC 1 cut(s) 918
Bpu1102I GCTNAGC 1 cut(s) 682
BpuMI CCSGG 2 cut(s) 27, 165
BsaAI YACGTR 1 cut(s) 226
BsaHI GRCGYC 1 cut(s) 186
BsaJI CCNNGG 4 cut(s) 25, 164, 181, 329
BsaWI WCCGGW 3 cut(s) 34, 319, 361
BsaXI ACNNNNNCTCC 6 cut(s) 356, 386, 483, 513, 833, 863
Bsc4I CCNNNNNNNGG 2 cut(s) 164, 934
Bse118I RCCGGY 2 cut(s) 319, 405
Bse1I ACTGG 2 cut(s) 171, 491
BseAI TCCGGA 1 cut(s) 361
BseBI CCWGG 2 cut(s) 182, 330
BseDI CCNNGG 4 cut(s) 25, 164, 181, 329
BseGI GGATG 2 cut(s) 352, 774
BseLI CCNNNNNNNGG 2 cut(s) 164, 934
BseMII CTCAG 4 cut(s) 276, 710, 747, 909
BseNI ACTGG 2 cut(s) 171, 491
BseRI GAGGAG 2 cut(s) 288, 854
BseSI GKGCMC 1 cut(s) 377
BseXI GCAGC 2 cut(s) 337, 545
Bsh1236I CGCG 1 cut(s) 113
BshFI GGCC 3 cut(s) 107, 212, 853
BshTI ACCGGT 1 cut(s) 319
BsiHKAI GWGCWC 1 cut(s) 377
BsiSI CCGG 7 cut(s) 27, 35, 164, 302, 320, 362, 406
BslFI GGGAC 3 cut(s) 159, 321, 727
BslI CCNNNNNNNGG 2 cut(s) 164, 934
BsmAI GTCTC 2 cut(s) 602, 736
BsmFI GGGAC 3 cut(s) 159, 321, 727
BsnI GGCC 3 cut(s) 107, 212, 853
Bsp1286I GDGCHC 1 cut(s) 377
Bsp13I TCCGGA 1 cut(s) 361
Bsp143I GATC 2 cut(s) 247, 742
Bsp1720I GCTNAGC 1 cut(s) 682
BspACI CCGC 8 cut(s) 8, 30, 111, 305, 450, 615, 782, 915
BspANI GGCC 3 cut(s) 107, 212, 853
BspCNI CTCAG 4 cut(s) 277, 711, 746, 910
BspEI TCCGGA 1 cut(s) 361
BspFNI CGCG 1 cut(s) 113
BspLI GGNNCC 5 cut(s) 124, 169, 202, 324, 445
BspMI ACCTGC 1 cut(s) 503
BsrFI RCCGGY 2 cut(s) 319, 405
BsrI ACTGG 2 cut(s) 171, 491
BssAI RCCGGY 2 cut(s) 319, 405
BssECI CCNNGG 4 cut(s) 25, 164, 181, 329
BssMI GATC 2 cut(s) 247, 742
BssNI GRCGYC 1 cut(s) 186
Bst2UI CCWGG 2 cut(s) 182, 330
Bst4CI ACNGT 3 cut(s) 150, 379, 883
Bst6I CTCTTC 1 cut(s) 600
BstACI GRCGYC 1 cut(s) 186
BstBAI YACGTR 1 cut(s) 226
BstC8I GCNNGC 7 cut(s) 355, 373, 407, 654, 855, 868, 947
BstDEI CTNAG 5 cut(s) 285, 682, 719, 733, 918
BstF5I GGATG 2 cut(s) 352, 774
BstFNI CGCG 1 cut(s) 113
BstH2I RGCGCY 2 cut(s) 729, 769
BstHHI GCGC 3 cut(s) 115, 728, 768
BstKTI GATC 2 cut(s) 250, 745
BstMAI GTCTC 2 cut(s) 602, 736
BstMBI GATC 2 cut(s) 247, 742
BstMWI GCNNNNNNNGC 4 cut(s) 302, 763, 863, 920
BstNI CCWGG 2 cut(s) 182, 330
BstSCI CCNGG 4 cut(s) 25, 163, 180, 328
BstSLI GKGCMC 1 cut(s) 377
BstUI CGCG 1 cut(s) 113
BstV1I GCAGC 2 cut(s) 337, 545
BstV2I GAAGAC 1 cut(s) 69
BstX2I RGATCY 1 cut(s) 742
BstXI CCANNNNNNTGG 2 cut(s) 785, 953
BstYI RGATCY 1 cut(s) 742
BsuRI GGCC 3 cut(s) 107, 212, 853
BtsCI GGATG 2 cut(s) 352, 774
BtsIMutI CAGTG 2 cut(s) 375, 716
BveI ACCTGC 1 cut(s) 503
Cac8I GCNNGC 7 cut(s) 355, 373, 407, 654, 855, 868, 947
CfoI GCGC 3 cut(s) 115, 728, 768
Cfr10I RCCGGY 2 cut(s) 319, 405
Cfr13I GGNCC 4 cut(s) 32, 40, 168, 384
CpoI CGGWCCG 1 cut(s) 32
CseI GACGC 1 cut(s) 175
Csp6I GTAC 3 cut(s) 50, 151, 459
CspAI ACCGGT 1 cut(s) 319
CspI CGGWCCG 1 cut(s) 32
CviAII CATG 1 cut(s) 437
CviQI GTAC 3 cut(s) 50, 151, 459
DdeI CTNAG 5 cut(s) 285, 682, 719, 733, 918
DpnI GATC 2 cut(s) 249, 744
DpnII GATC 2 cut(s) 247, 742
DraI TTTAAA 1 cut(s) 664
DraIII CACNNNGTG 1 cut(s) 722
DriI GACNNNNNGTC 1 cut(s) 38
Eam1104I CTCTTC 1 cut(s) 600
Eam1105I GACNNNNNGTC 1 cut(s) 38
EarI CTCTTC 1 cut(s) 600
EciI GGCGGA 1 cut(s) 45
Eco47I GGWCC 4 cut(s) 32, 40, 168, 384
Eco57I CTGAAG 1 cut(s) 930
Eco72I CACGTG 1 cut(s) 226
EcoO109I RGGNCCY 1 cut(s) 40
EcoRII CCWGG 2 cut(s) 180, 328
FaeI CATG 1 cut(s) 440
FaiI YATR 5 cut(s) 282, 398, 438, 752, 951
FaqI GGGAC 3 cut(s) 159, 321, 727
FatI CATG 1 cut(s) 436
FauI CCCGC 1 cut(s) 298
FbaI TGATCA 1 cut(s) 247
FblI GTMKAC 1 cut(s) 888
Fnu4HI GCNGC 5 cut(s) 351, 534, 616, 783, 915
FokI GGATG 2 cut(s) 359, 761
Fsp4HI GCNGC 5 cut(s) 351, 534, 616, 783, 915
FspBI CTAG 1 cut(s) 942
GlaI GCGC 3 cut(s) 114, 727, 767
GluI GCNGC 5 cut(s) 351, 534, 616, 783, 915
HaeII RGCGCY 2 cut(s) 729, 769
HaeIII GGCC 3 cut(s) 107, 212, 853
HapII CCGG 7 cut(s) 27, 35, 164, 302, 320, 362, 406
HgaI GACGC 1 cut(s) 175
HhaI GCGC 3 cut(s) 115, 728, 768
Hin1I GRCGYC 1 cut(s) 186
Hin1II CATG 1 cut(s) 440
Hin6I GCGC 3 cut(s) 113, 726, 766
HinP1I GCGC 3 cut(s) 113, 726, 766
HinfI GANTC 2 cut(s) 453, 795
HpaII CCGG 7 cut(s) 27, 35, 164, 302, 320, 362, 406
HphI GGTGA 4 cut(s) 257, 763, 886, 917
Hpy166II GTNNAC 3 cut(s) 375, 387, 889
Hpy188I TCNGA 7 cut(s) 47, 98, 138, 474, 736, 800, 910
Hpy188III TCNNGA 5 cut(s) 198, 217, 231, 362, 806
Hpy8I GTNNAC 3 cut(s) 375, 387, 889
Hpy99I CGWCG 3 cut(s) 86, 191, 218
HpyAV CCTTC 3 cut(s) 136, 283, 337
HpyCH4III ACNGT 3 cut(s) 150, 379, 883
HpyCH4IV ACGT 3 cut(s) 225, 558, 846
HpyCH4V TGCA 2 cut(s) 375, 857
HpyF10VI GCNNNNNNNGC 4 cut(s) 302, 763, 863, 920
HpyF3I CTNAG 5 cut(s) 285, 682, 719, 733, 918
HpySE526I ACGT 3 cut(s) 225, 558, 846
Hsp92I GRCGYC 1 cut(s) 186
Hsp92II CATG 1 cut(s) 440
HspAI GCGC 3 cut(s) 113, 726, 766
Kpn2I TCCGGA 1 cut(s) 361
KroI GCCGGC 1 cut(s) 405
KroNI GCCGGC 1 cut(s) 407
Ksp22I TGATCA 1 cut(s) 247
Kzo9I GATC 2 cut(s) 247, 742
LmnI GCTCC 2 cut(s) 122, 544
Lsp1109I GCAGC 2 cut(s) 337, 545
LweI GCATC 1 cut(s) 337
MaeI CTAG 1 cut(s) 942
MaeII ACGT 3 cut(s) 225, 558, 846
MaeIII GTNAC 5 cut(s) 16, 173, 505, 589, 668
MalI GATC 2 cut(s) 249, 744
MboI GATC 2 cut(s) 247, 742
MboII GAAGA 4 cut(s) 74, 405, 617, 737
MfeI CAATTG 1 cut(s) 858
MflI RGATCY 1 cut(s) 742
MhlI GDGCHC 1 cut(s) 377
MluCI AATT 3 cut(s) 568, 822, 858
MroI TCCGGA 1 cut(s) 361
MroNI GCCGGC 1 cut(s) 405
MseI TTAA 2 cut(s) 267, 663
MslI CAYNNNNRTG 1 cut(s) 395
MspA1I CMGCKG 2 cut(s) 450, 917
MspI CCGG 7 cut(s) 27, 35, 164, 302, 320, 362, 406
MspR9I CCNGG 4 cut(s) 27, 165, 182, 330
MunI CAATTG 1 cut(s) 858
MvaI CCWGG 2 cut(s) 182, 330
MvnI CGCG 1 cut(s) 113
MwoI GCNNNNNNNGC 4 cut(s) 302, 763, 863, 920
NaeI GCCGGC 1 cut(s) 407
NciI CCSGG 2 cut(s) 27, 165
NdeII GATC 2 cut(s) 247, 742
NgoMIV GCCGGC 1 cut(s) 405
NlaIII CATG 1 cut(s) 440
NlaIV GGNNCC 5 cut(s) 124, 169, 202, 324, 445
NmuCI GTSAC 2 cut(s) 173, 505
PcsI WCGNNNNNNNCGW 1 cut(s) 222
PdiI GCCGGC 1 cut(s) 407
PfeI GAWTC 2 cut(s) 453, 795
PflMI CCANNNNNTGG 1 cut(s) 934
PinAI ACCGGT 1 cut(s) 319
PkrI GCNGC 5 cut(s) 352, 535, 617, 784, 916
PmaCI CACGTG 1 cut(s) 226
PmlI CACGTG 1 cut(s) 226
Ppu21I YACGTR 1 cut(s) 226
PpuMI RGGWCCY 1 cut(s) 40
Psp5II RGGWCCY 1 cut(s) 40
Psp6I CCWGG 2 cut(s) 180, 328
PspCI CACGTG 1 cut(s) 226
PspGI CCWGG 2 cut(s) 180, 328
PspN4I GGNNCC 5 cut(s) 124, 169, 202, 324, 445
PspPI GGNCC 4 cut(s) 32, 40, 168, 384
PspPPI RGGWCCY 1 cut(s) 40
PsuI RGATCY 1 cut(s) 742
RsaI GTAC 3 cut(s) 51, 152, 460
RsaNI GTAC 3 cut(s) 50, 151, 459
RseI CAYNNNNRTG 1 cut(s) 395
Rsr2I CGGWCCG 1 cut(s) 32
RsrII CGGWCCG 1 cut(s) 32
SaqAI TTAA 2 cut(s) 267, 663
SatI GCNGC 5 cut(s) 351, 534, 616, 783, 915
Sau3AI GATC 2 cut(s) 247, 742
Sau96I GGNCC 4 cut(s) 32, 40, 168, 384
ScrFI CCNGG 4 cut(s) 27, 165, 182, 330
SduI GDGCHC 1 cut(s) 377
SfaNI GCATC 1 cut(s) 337
SinI GGWCC 4 cut(s) 32, 40, 168, 384
SmiMI CAYNNNNRTG 1 cut(s) 395
Sse9I AATT 3 cut(s) 568, 822, 858
SsiI CCGC 8 cut(s) 8, 30, 111, 305, 450, 615, 782, 915
SspMI CTAG 1 cut(s) 942
StyD4I CCNGG 4 cut(s) 25, 163, 180, 328
TaaI ACNGT 3 cut(s) 150, 379, 883
TaiI ACGT 3 cut(s) 228, 561, 849
TaqI TCGA 2 cut(s) 189, 207
TasI AATT 3 cut(s) 568, 822, 858
TatI WGTACW 1 cut(s) 49
TauI GCSGC 3 cut(s) 618, 785, 917
TfiI GAWTC 2 cut(s) 453, 795
Tru1I TTAA 2 cut(s) 267, 663
Tru9I TTAA 2 cut(s) 267, 663
TscAI CASTG 2 cut(s) 382, 723
TseFI GTSAC 2 cut(s) 173, 505
TseI GCWGC 2 cut(s) 350, 533
Tsp45I GTSAC 2 cut(s) 173, 505
TspDTI ATGAA 2 cut(s) 17, 425
TspGWI ACGGA 3 cut(s) 135, 593, 963
TspRI CASTG 2 cut(s) 382, 723
Van91I CCANNNNNTGG 1 cut(s) 934
VneI GTGCAC 1 cut(s) 373
VpaK11BI GGWCC 4 cut(s) 32, 40, 168, 384
XcmI CCANNNNNNNNNTGG 1 cut(s) 178
XmiI GTMKAC 1 cut(s) 888
XspI CTAG 1 cut(s) 942
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.