Rh1AG037400

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0004242
Physical Location & Seq
Forward (+)
6591218 .. 6596996
5779 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG037400.1

Sequence Viewer

Length: 4305 bp
ATGCCTATTGTTCGAGCACTTGAATCTGCTTCTGTAAGCATCCTCCTTAACAAGTTGGCCACTCAAGATGTCATAGATTTCTTCCTCAAGTGGGAAATCGATGGCTCCCTGCTAGCAAAGCTCAAGAGAAACCTACTTGTAATTCATGCAGTTCTCAATCATGCTGAGGAGAAGCAAGTCCAGGACTCAAATGTCAAAGCTTGGCTTGAGGACGTCAGAGTTGCAGCTTATGATGCCGAGGACATATTGGATGACATCGCAAATGATGCTCTTGAATCACAAAACAGCAAACATGAGGTATGGAATTCTAAGATCAAAGAAGGGATAGACTTTAAGATCAGAGACATTGCTAATACTCTTAATCCTTTTAGAGAACGTGTAGAGTCGAAAATGATAAAGATTATAGATATATTTGAAGAGATTGCAAAACAGAAAGATATTCTTCGTTTGAGAGAAGATTCTGGCGGTATAGAATCAGGTTTTGACAGATTGCCCACGACTCCAATGTTGGGTGATGAATCTCATGTTTATGGTAGAGAATCTGATAAGGAGGAGATAATTACTTTGCTGAATTTGGGTGGAAATGATGATGGGGTTTCTATAATCCCAATTGTAGGCATGGGAGGTATTGGGAAGACTACACTTGCTCAGCTTGTGTATAATGATGCTAATGTGAGTAGCCATTTTGATTTGAAAGCCTGGGCTTGTGTCTCTGACGTATTTGATGTGCAAAGGATAACAAAAACACTTGTTGAGTCTGCGACTAAATCAAGTTGTAGCACAAACAATTTGGAGTTACTTCAAGAACAACTGAAAATACGGTTGAAGGATACGAAATTTCTTTTTGTTCTGGATGATGTCTGGAACGAGAAGTATGAAAGCTGGGATAAGTTAAGAGTCCCCTTCACAGTTGGTGCACCAGGAAGTAAAATCATAATCACGACACGAAACAAAACAGTTGCATCACTTATGGGGACTGTTCCTACTTACTGTTTGGAGGGGCTTTCAGACAGTGATTGTTGGTTATTATTCGAACAAATTGTGTTTCAGAACAGGAATCTAGATGCCTATCCAAAGTTGAAAGTGATTGGCAAGAAAATAGTGGACAAGTGTAAAGGGTTGCCTTTGGCTGCAAAGGCACTAGGAGGTCTCCTGCGTGCTGAACCGGAACTGGATGAGAATTACTGGAATGATATCTTGAATAGCAAACTTTGGAAACTGGCAGACAACACTATTCTACCAGCTCTAAGATTAAGCTACCATCACCTCCCTGGGCATTTGAAGCGCTGCTTTGCTTATTGCTCGATGTTTCCTAAGGACTATGAATATGAAAGAGAGATATTAATCTTGTTATGGATGGCAGAGGGTTTTGTGCCAGAGCCAGAAGGAAATCAACAAATCGAAGATGTAGCTGGAAGATATGTTTCAGATTTTTTATCAAGGTCCTTCTTCCAACACACCAGTGACAAGTCACGACTGGTAATGCATGACCTCATACATGATCTTGCACAATCTGTTTCTGGCAAAACATTTGTTAGGTTGGAGGAAAATTCAGAGAACCATAAAAAGTCTACAAAAGCTCGGCACTTGTCATATACCCGTGGATCTGAAGATGTATTTCAGAAATTTGAGGCATTCAGTGAGGTGGATTGTTTAAGAACCTTTCTACCACTAGACCCGTTGCAAGGATTCAATATGAGTAGCTTAACTGACAAGGTTCCTTGTGATATGATGCCAAAATTAAGATTCCTGCGGGTGCTATCCTTTAGCGGTTATCTCATAGAAAAGTTGCCAGATTCAATTGGCAACTTGAAGCATCTGCGATACCTCAATCTTTCTCATTGTCAAATAGAAGAGCTACCTGAGCCAACAAGCAGTCTATACAATCTGCAGACATTAATTTTATTCAGATGTCGATTGCTTACCATGCTGCCTGCAGACATAGGAAACCTAAGGAATTTACGGCATCTCAATATTCAGGGAACTAGGCTGAAAAGTATGCCTTTGGGAATGGGAAGATTGGTTAACCTTCAAATACTGTCAGATTTTATTGTGGGGAAAGCCATGGGAAAAGGGATTGCAGAATTGAAGGACTTGTCACATCTTCGAGGATCAGTTTCTATTTTGTGTTTGCATGATGTCGGCAGTGTTACGGATGCAATTAAGGCCAGATTAGAGGTTAAGAAGCACCTGGATGATTTAGTTTTGGAATGGAGCAGCAGTACTGATGGTTCACGAAATGAAGGGATCGAGACAGAGGTACTTGATGCACTGCAGCCTCATGAAAACCTGAAGAAACTCACCATTAAGTATTATGGAGGTACAGAATTTCCATATTGGATGGGGAATCCTTTGTTCACTAACATGGCGTACTTGCATCTCTATGGTTGCATAAAATGCCCATCTTTACCACCCCTTGGCCAACTACCTTCTCTTAAAGATCTCATAATTGAAGCAATGCATGGAATACAGTGTGTAGGAATGGATTTTTTTGGGGATGATGATGGTTCCACAATTTTTTTCCCCTCTTTGGAGACTTTGAAATTTGAGGACATGAAAGAATGGGAGAAGTGGTCATCTTCAGAAGATGAAAGGCTTAAAGGATTTCCCAGCCTTCGGGAGCTTTCCATATTTAGATGTCCCAAGTTAACAAAGTTTACACATGGATTTGCTTCTCTAGAAAAGCTGCGCATCAAGAACTGCAGAGCAATAACTACCTTCTCACAGAATCCTGCACTTGGGAATTTGGAACCTGTAGAATTCCCTAGCCTGCAACTGCTTGTTCTTGTTGGTTGCAGTGAGCTTGAAAACCTTCCTTTTTCCCTCCCTTCGTTGAAAGTTCTTGAGATAGATGGTTGTGAAAAATTGGCTGCACTTCCAAGGATGGTGGAACTTAGTACATTGTACCTGATCGATTCAAATGCTGAATTACTTGGATGGATTATGGAATTTAGCTCGCTGACCTCTTTGCACCTAACTCATATTCCACATTTGAAGCATCTACCTGAAGGCTTTATCCAACAGTCTGCAAAACTTGAAGAATTGAGCCTTGCAGGCTTTCCTGACCTTGAATATTTGGTAGATGACCGAGTTGGACTAGCACACCTTGTATCCCTTCAACGCTTGACAATATCCCATTGTCCTGAGTTTATTGCTTTGCCTGATGAAGATGATCAGCTCCCACCTGTACTTAAATATTTGGATTTGAAACATTGTCCTAGCCTGATGAAGCTGCCGGGTAAACTTCACAACCTTGAGTGTCTTGCTGAGCTGCAGATTGAATGGTGTTCAAAGATTGAAGCATTTCCAGATGTGGGGTTGCCTGACAAACTGAAGCGTCTTGTAGTCCGCGAATGTGGGGCTCTGAAGACCCTTCCTCAGGAGCTTCTCTGCAATAACAATTTTCTCGAGTATTTAGAAGTTCATAAATGTCCTTCTCTTGTGTCAGTTCTTGAAGAAGGAAATTTGCCATCAACTCTAACACATATGAAATTTTACTATTGCAAAATTCTGGGATCACTGCCTGAGGGTTTGATGCACAAAGACAATATGACTCTAAAGTACTTGGAGATAGACAACTGTTCCTCTCTCATGTCCTTTCCAAATGGTGAGTTACCAACTAGTCTTGAGCGTCTTGAGATTAGTGATTGCTCTAATTTCCAGACATTGCCATTGAGCCTTCTCAACCTCACGAATCTTGTGACCTTGGAAGTAAAGAGCTGCCCCCTTCTTCAGGACTTTCCAGAGGGTGGATTGCCACCCAACATAAAGTTTGTCACAATTTTCGAATGTGAGAATCTCAAGTCTCTACCTAAATTATTTCATAAACTCAAATGTCTTCAAAAGTTGGAGATTTCTGGCTGTCCTAGTCTCCTGTCCTTGCCAAAGCAGGGTCTGCCCAGCAGCTTAAGAGTACTTACAGTTACTGATTGTGAAAAGCTCAATCCCATAGATCAGTGGAGACTTCACAAACTACCCTCTCTAGATGTTTTCACCATTGGTGGGTTTCCTGGCCTGAAGTCCTTTTCAAAAAGTTATCCTCTTCCTCACACTTTAACCTCTTTAAGCATTCAAAGACTCCCAGATCTTGAATCTATATCGGAGGTGCTAGAGAATCTCACCTCACTTGAAAAGCTGAGCATCAGGGATTGTGATAAGCTCCAATCTTTGCCAGAGACGGGGCTACCAGCGACAATTTCTACCTTGACAATTCGGTATTGTTTGCTTCTAGAACATCGATGTGAAAAGGACAAAGGAGAAGATTGGTCCAAGATCAAAAACATCCCCTTTGTGAATTCACGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

1434

Amino Acids

161.97

Weight (kDa)

5.63

Isoelectric Point (pI)

44.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 12 - 98 7.9e-17 Rx N-terminal domain
NB-ARC PF00931 180 - 352 5e-36 NB-ARC domain
WHD_DRP PF23559 436 - 503 2.7e-24 Disease resistance protein Winged helix domain
LRR_14 PF23598 577 - 850 2e-30 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 693 - 820 1.6e-38 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 191
AatII GACGTC 1 cut(s) 216
Acc16I TGCGCA 1 cut(s) 2690
AccB7I CCANNNNNTGG 2 cut(s) 2417, 3749
AccI GTMKAC 1 cut(s) 1571
AccII CGCG 1 cut(s) 3351
AciI CCGC 4 cut(s) 465, 1753, 1770, 3349
AclWI GGATC 4 cut(s) 1612, 2119, 2255, 3523
AcoI YGGCCR 2 cut(s) 57, 2419
AcuI CTGAAG 8 cut(s) 1629, 2312, 2565, 3027, 3353, 3386, 3716, 4037
AcyI GRCGYC 1 cut(s) 213
AfaI GTAC 9 cut(s) 2224, 2262, 2323, 2372, 2899, 2906, 3189, 3563, 3915
AfeI AGCGCT 1 cut(s) 1286
AflII CTTAAG 1 cut(s) 3907
AflIII ACRYGT 1 cut(s) 376
AhlI ACTAGT 1 cut(s) 3620
AjnI CCWGG 6 cut(s) 180, 698, 919, 1270, 2190, 4009
AleI CACNNNNGTG 1 cut(s) 1461
Alw21I GWGCWC 2 cut(s) 19, 919
Alw26I GTCTC 9 cut(s) 336, 715, 1154, 2246, 2528, 3810, 3875, 3955, 4169
Alw44I GTGCAC 1 cut(s) 915
AlwI GGATC 4 cut(s) 1612, 2119, 2255, 3523
AlwNI CAGNNNCTG 2 cut(s) 3895, 3926
Ama87I CYCGRG 1 cut(s) 3407
Aor51HI AGCGCT 1 cut(s) 1286
AoxI GGCC 4 cut(s) 57, 2166, 2419, 4012
ApaLI GTGCAC 1 cut(s) 915
AseI ATTAAT 2 cut(s) 1343, 1898
Asp700I GAANNNNTTC 2 cut(s) 2811, 3303
AspLEI GCGC 2 cut(s) 1287, 2691
AspS9I GGNCC 2 cut(s) 1443, 4266
AsuC2I CCSGG 1 cut(s) 3237
AsuHPI GGTGA 6 cut(s) 524, 1256, 2293, 3620, 3985, 4111
AsuII TTCGAA 2 cut(s) 1032, 3786
AsuNHI GCTAGC 1 cut(s) 112
AvaI CYCGRG 1 cut(s) 3407
AvaII GGWCC 2 cut(s) 1443, 4266
AxyI CCTNAGG 4 cut(s) 1314, 1952, 3378, 3525
BaeGI GKGCMC 1 cut(s) 919
BalI TGGCCA 2 cut(s) 59, 2421
BanII GRGCYC 1 cut(s) 3364
BbsI GAAGAC 3 cut(s) 641, 3374, 3830
Bbv12I GWGCWC 2 cut(s) 19, 919
BbvCI CCTCAGC 1 cut(s) 165
BceAI ACGGC 1 cut(s) 1979
BcgI CGANNNNNNTGC 2 cut(s) 2903, 2937
BciT130I CCWGG 6 cut(s) 182, 700, 921, 1272, 2192, 4011
BciVI GTATCC 2 cut(s) 823, 3121
BclI TGATCA 1 cut(s) 3172
BcnI CCSGG 1 cut(s) 3237
BcoDI GTCTC 9 cut(s) 336, 715, 1154, 2246, 2528, 3810, 3875, 3955, 4169
BcuI ACTAGT 1 cut(s) 3620
BfmI CTRYAG 6 cut(s) 1889, 1935, 2273, 2701, 2754, 3272
BfoI RGCGCY 1 cut(s) 1288
BfrI CTTAAG 1 cut(s) 3907
BfuI GTATCC 2 cut(s) 823, 3121
BglI GCCNNNNNGGC 1 cut(s) 3054
BglII AGATCT 2 cut(s) 2440, 4084
BlpI GCTNAGC 3 cut(s) 648, 3267, 4136
BmcAI AGTACT 3 cut(s) 2224, 3563, 3915
Bme1390I CCNGG 7 cut(s) 182, 700, 921, 1272, 2192, 3237, 4011
Bme18I GGWCC 2 cut(s) 1443, 4266
BmeT110I CYCGRG 1 cut(s) 3407
BmgT120I GGNCC 2 cut(s) 1443, 4266
BmiI GGNNCC 4 cut(s) 106, 1719, 2509, 2751
BmrFI CCNGG 7 cut(s) 182, 700, 921, 1272, 2192, 3237, 4011
BmtI GCTAGC 1 cut(s) 116
BpiI GAAGAC 3 cut(s) 641, 3374, 3830
Bpu10I CCTNAGC 2 cut(s) 165, 1863
Bpu1102I GCTNAGC 3 cut(s) 648, 3267, 4136
Bpu14I TTCGAA 2 cut(s) 1032, 3786
BpuEI CTTGAG 9 cut(s) 48, 71, 107, 227, 2864, 3275, 3647, 3656, 3785
BpuMI CCSGG 1 cut(s) 3237
Bsa29I ATCGAT 3 cut(s) 99, 2913, 4237
BsaBI GATNNNNATC 2 cut(s) 1068, 1343
BsaHI GRCGYC 1 cut(s) 213
BsaI GGTCTC 1 cut(s) 1154
BsaJI CCNNGG 9 cut(s) 237, 699, 1270, 1271, 1600, 2064, 2416, 2879, 3705
BsaWI WCCGGW 1 cut(s) 1165
BsaXI ACNNNNNCTCC 4 cut(s) 2206, 2236, 2558, 2588
Bse1I ACTGG 5 cut(s) 1176, 1190, 1224, 1461, 1482
Bse21I CCTNAGG 4 cut(s) 1314, 1952, 3378, 3525
Bse3DI GCAATG 3 cut(s) 345, 2463, 3665
Bse8I GATNNNNATC 2 cut(s) 1068, 1343
BseBI CCWGG 6 cut(s) 182, 700, 921, 1272, 2192, 4011
BseCI ATCGAT 3 cut(s) 99, 2913, 4237
BseDI CCNNGG 9 cut(s) 237, 699, 1270, 1271, 1600, 2064, 2416, 2879, 3705
BseJI GATNNNNATC 2 cut(s) 1068, 1343
BseMI GCAATG 3 cut(s) 345, 2463, 3665
BseMII CTCAG 8 cut(s) 156, 662, 1854, 3135, 3258, 3392, 3516, 4127
BseNI ACTGG 5 cut(s) 1176, 1190, 1224, 1461, 1482
BseRI GAGGAG 2 cut(s) 182, 566
BseSI GKGCMC 1 cut(s) 919
BseYI CCCAGC 3 cut(s) 882, 2609, 3899
BsgI GTGCAG 2 cut(s) 2718, 2856
Bsh1236I CGCG 1 cut(s) 3351
BshFI GGCC 4 cut(s) 59, 2168, 2421, 4014
BshVI ATCGAT 3 cut(s) 99, 2913, 4237
BsiHKAI GWGCWC 2 cut(s) 19, 919
BsiHKCI CYCGRG 1 cut(s) 3407
BsiSI CCGG 2 cut(s) 1166, 3236
BslFI GGGAC 3 cut(s) 884, 988, 2625
BsmAI GTCTC 9 cut(s) 336, 715, 1154, 2246, 2528, 3810, 3875, 3955, 4169
BsmBI CGTCTC 1 cut(s) 4169
BsmFI GGGAC 3 cut(s) 884, 988, 2625
BsmI GAATGC 2 cut(s) 1634, 4068
BsnI GGCC 4 cut(s) 59, 2168, 2421, 4014
Bso31I GGTCTC 1 cut(s) 1154
BsoBI CYCGRG 1 cut(s) 3407
Bsp119I TTCGAA 2 cut(s) 1032, 3786
Bsp1286I GDGCHC 3 cut(s) 19, 919, 3364
Bsp1720I GCTNAGC 3 cut(s) 648, 3267, 4136
Bsp19I CCATGG 1 cut(s) 2064
BspACI CCGC 4 cut(s) 465, 1753, 1770, 3349
BspANI GGCC 4 cut(s) 59, 2168, 2421, 4014
BspCNI CTCAG 8 cut(s) 157, 661, 1855, 3136, 3259, 3391, 3517, 4128
BspDI ATCGAT 3 cut(s) 99, 2913, 4237
BspFNI CGCG 1 cut(s) 3351
BspHI TCATGA 1 cut(s) 2281
BspLI GGNNCC 4 cut(s) 106, 1719, 2509, 2751
BspMAI CTGCAG 5 cut(s) 1893, 1939, 2277, 2705, 3276
BspOI GCTAGC 1 cut(s) 116
BspPI GGATC 4 cut(s) 1612, 2119, 2255, 3523
BspQI GCTCTTC 1 cut(s) 1848
BspT104I TTCGAA 2 cut(s) 1032, 3786
BspTI CTTAAG 1 cut(s) 3907
BspTNI GGTCTC 1 cut(s) 1154
BsrDI GCAATG 3 cut(s) 345, 2463, 3665
BsrI ACTGG 5 cut(s) 1176, 1190, 1224, 1461, 1482
BssECI CCNNGG 9 cut(s) 237, 699, 1270, 1271, 1600, 2064, 2416, 2879, 3705
BssNI GRCGYC 1 cut(s) 213
BssT1I CCWWGG 4 cut(s) 2064, 2416, 2879, 3705
Bst2UI CCWGG 6 cut(s) 182, 700, 921, 1272, 2192, 4011
Bst6I CTCTTC 3 cut(s) 411, 1848, 4047
BstACI GRCGYC 1 cut(s) 213
BstAFI CTTAAG 1 cut(s) 3907
BstAPI GCANNNNNTGC 3 cut(s) 266, 2397, 3895
BstBI TTCGAA 2 cut(s) 1032, 3786
BstC8I GCNNGC 6 cut(s) 114, 1158, 1935, 2771, 2957, 3055
BstDSI CCRYGG 2 cut(s) 1600, 2064
BstENI CCTNNNNNAGG 2 cut(s) 3377, 3731
BstFNI CGCG 1 cut(s) 3351
BstH2I RGCGCY 1 cut(s) 1288
BstHHI GCGC 2 cut(s) 1287, 2691
BstMAI GTCTC 9 cut(s) 336, 715, 1154, 2246, 2528, 3810, 3875, 3955, 4169
BstNI CCWGG 6 cut(s) 182, 700, 921, 1272, 2192, 4011
BstSCI CCNGG 7 cut(s) 180, 698, 919, 1270, 2190, 3235, 4009
BstSFI CTRYAG 6 cut(s) 1889, 1935, 2273, 2701, 2754, 3272
BstSLI GKGCMC 1 cut(s) 919
BstUI CGCG 1 cut(s) 3351
BstV2I GAAGAC 3 cut(s) 641, 3374, 3830
BstX2I RGATCY 3 cut(s) 1604, 2440, 4084
BstYI RGATCY 3 cut(s) 1604, 2440, 4084
Bsu15I ATCGAT 3 cut(s) 99, 2913, 4237
Bsu36I CCTNAGG 4 cut(s) 1314, 1952, 3378, 3525
BsuI GTATCC 2 cut(s) 823, 3121
BsuRI GGCC 4 cut(s) 59, 2168, 2421, 4014
BsuTUI ATCGAT 3 cut(s) 99, 2913, 4237
BtgI CCRYGG 2 cut(s) 1600, 2064
BtgZI GCGATG 1 cut(s) 241
BtsI GCAGTG 4 cut(s) 2152, 2270, 2803, 3518
BtsIMutI CAGTG 9 cut(s) 1018, 1468, 1645, 2152, 2270, 2477, 2803, 3518, 3962
Cac8I GCNNGC 6 cut(s) 114, 1158, 1935, 2771, 2957, 3055
CaiI CAGNNNCTG 2 cut(s) 3895, 3926
CciI TCATGA 1 cut(s) 2281
CfoI GCGC 2 cut(s) 1287, 2691
Cfr13I GGNCC 2 cut(s) 1443, 4266
ClaI ATCGAT 3 cut(s) 99, 2913, 4237
CseI GACGC 2 cut(s) 3326, 3620
Csp6I GTAC 9 cut(s) 2223, 2261, 2322, 2371, 2898, 2905, 3188, 3562, 3914
CspCI CAANNNNNGTGG 2 cut(s) 2868, 2903
CviQI GTAC 9 cut(s) 2223, 2261, 2322, 2371, 2898, 2905, 3188, 3562, 3914
DrdI GACNNNNNNGTC 1 cut(s) 191
DseDI GACNNNNNNGTC 1 cut(s) 191
EaeI YGGCCR 2 cut(s) 57, 2419
Eam1104I CTCTTC 3 cut(s) 411, 1848, 4047
EarI CTCTTC 3 cut(s) 411, 1848, 4047
Eco130I CCWWGG 4 cut(s) 2064, 2416, 2879, 3705
Eco24I GRGCYC 1 cut(s) 3364
Eco31I GGTCTC 1 cut(s) 1154
Eco32I GATATC 1 cut(s) 1195
Eco47I GGWCC 2 cut(s) 1443, 4266
Eco47III AGCGCT 1 cut(s) 1286
Eco57I CTGAAG 8 cut(s) 1629, 2312, 2565, 3027, 3353, 3386, 3716, 4037
Eco81I CCTNAGG 4 cut(s) 1314, 1952, 3378, 3525
Eco88I CYCGRG 1 cut(s) 3407
EcoNI CCTNNNNNAGG 2 cut(s) 3377, 3731
EcoO109I RGGNCCY 1 cut(s) 1443
EcoRI GAATTC 3 cut(s) 304, 2759, 4294
EcoRII CCWGG 6 cut(s) 180, 698, 919, 1270, 2190, 4009
EcoRV GATATC 1 cut(s) 1195
EcoT14I CCWWGG 4 cut(s) 2064, 2416, 2879, 3705
EcoT22I ATGCAT 2 cut(s) 1488, 2463
EcoT38I GRGCYC 1 cut(s) 3364
ErhI CCWWGG 4 cut(s) 2064, 2416, 2879, 3705
Esp3I CGTCTC 1 cut(s) 4169
FalI AAGNNNNNCTT 8 cut(s) 189, 221, 426, 458, 1274, 1306, 1987, 2019
FaqI GGGAC 3 cut(s) 884, 988, 2625
FauI CCCGC 1 cut(s) 1746
FauNDI CATATG 1 cut(s) 3486
FbaI TGATCA 1 cut(s) 3172
FblI GTMKAC 1 cut(s) 1571
FriOI GRGCYC 1 cut(s) 3364
FspI TGCGCA 1 cut(s) 2690
GlaI GCGC 2 cut(s) 1286, 2690
GsaI CCCAGC 3 cut(s) 886, 2613, 3903
HaeII RGCGCY 1 cut(s) 1288
HaeIII GGCC 4 cut(s) 59, 2168, 2421, 4014
HapII CCGG 2 cut(s) 1166, 3236
HgaI GACGC 2 cut(s) 3326, 3620
HhaI GCGC 2 cut(s) 1287, 2691
Hin1I GRCGYC 1 cut(s) 213
Hin6I GCGC 2 cut(s) 1285, 2689
HinP1I GCGC 2 cut(s) 1285, 2689
HincII GTYRAC 2 cut(s) 2026, 2649
HindII GTYRAC 2 cut(s) 2026, 2649
HindIII AAGCTT 1 cut(s) 198
HpaI GTTAAC 2 cut(s) 2026, 2649
HpaII CCGG 2 cut(s) 1166, 3236
HphI GGTGA 6 cut(s) 524, 1256, 2293, 3620, 3985, 4111
Hpy166II GTNNAC 9 cut(s) 917, 1105, 1572, 2026, 2234, 2358, 2649, 2658, 3242
Hpy8I GTNNAC 9 cut(s) 917, 1105, 1572, 2026, 2234, 2358, 2649, 2658, 3242
HpyCH4IV ACGT 3 cut(s) 213, 376, 717
HpySE526I ACGT 3 cut(s) 213, 376, 717
Hsp92I GRCGYC 1 cut(s) 213
HspAI GCGC 2 cut(s) 1285, 2689
Ksp22I TGATCA 1 cut(s) 3172
KspAI GTTAAC 2 cut(s) 2026, 2649
LguI GCTCTTC 1 cut(s) 1848
LmnI GCTCC 6 cut(s) 110, 2214, 2620, 3183, 3382, 4164
MaeII ACGT 3 cut(s) 213, 376, 717
MaeIII GTNAC 9 cut(s) 795, 1463, 1470, 2097, 2149, 3612, 3700, 3775, 3922
MfeI CAATTG 2 cut(s) 609, 1800
MflI RGATCY 3 cut(s) 1604, 2440, 4084
MhlI GDGCHC 3 cut(s) 19, 919, 3364
MlsI TGGCCA 2 cut(s) 59, 2421
MluNI TGGCCA 2 cut(s) 59, 2421
MlyI GAGTC 7 cut(s) 179, 392, 493, 764, 906, 3547, 4071
MmeI TCCRAC 5 cut(s) 1477, 1521, 3043, 3073, 3828
Mox20I TGGCCA 2 cut(s) 59, 2421
Mph1103I ATGCAT 2 cut(s) 1488, 2463
MroXI GAANNNNTTC 2 cut(s) 2811, 3303
MscI TGGCCA 2 cut(s) 59, 2421
MslI CAYNNNNRTG 6 cut(s) 528, 1461, 2193, 2363, 2382, 4239
Msp20I TGGCCA 2 cut(s) 59, 2421
MspCI CTTAAG 1 cut(s) 3907
MspI CCGG 2 cut(s) 1166, 3236
MspR9I CCNGG 7 cut(s) 182, 700, 921, 1272, 2192, 3237, 4011
MunI CAATTG 2 cut(s) 609, 1800
Mva1269I GAATGC 2 cut(s) 1634, 4068
MvaI CCWGG 6 cut(s) 182, 700, 921, 1272, 2192, 4011
MvnI CGCG 1 cut(s) 3351
NciI CCSGG 1 cut(s) 3237
NcoI CCATGG 1 cut(s) 2064
NdeI CATATG 1 cut(s) 3486
NheI GCTAGC 1 cut(s) 112
NlaIV GGNNCC 4 cut(s) 106, 1719, 2509, 2751
NmeAIII GCCGAG 2 cut(s) 262, 1561
NmuCI GTSAC 5 cut(s) 1463, 1470, 2097, 3700, 3775
NsbI TGCGCA 1 cut(s) 2690
NsiI ATGCAT 2 cut(s) 1488, 2463
NspV TTCGAA 2 cut(s) 1032, 3786
OliI CACNNNNGTG 1 cut(s) 1461
PaeR7I CTCGAG 1 cut(s) 3407
PagI TCATGA 1 cut(s) 2281
PasI CCCWGGG 1 cut(s) 1271
PciSI GCTCTTC 1 cut(s) 1848
PctI GAATGC 2 cut(s) 1634, 4068
PdmI GAANNNNTTC 2 cut(s) 2811, 3303
PflMI CCANNNNNTGG 2 cut(s) 2417, 3749
PfoI TCCNGGA 1 cut(s) 180
PleI GAGTC 7 cut(s) 179, 391, 493, 763, 905, 3547, 4071
PpsI GAGTC 7 cut(s) 179, 391, 493, 763, 905, 3547, 4071
PpuMI RGGWCCY 1 cut(s) 1443
PshBI ATTAAT 2 cut(s) 1343, 1898
Psp5II RGGWCCY 1 cut(s) 1443
Psp6I CCWGG 6 cut(s) 180, 698, 919, 1270, 2190, 4009
PspFI CCCAGC 3 cut(s) 882, 2609, 3899
PspGI CCWGG 6 cut(s) 180, 698, 919, 1270, 2190, 4009
PspN4I GGNNCC 4 cut(s) 106, 1719, 2509, 2751
PspPI GGNCC 2 cut(s) 1443, 4266
PspPPI RGGWCCY 1 cut(s) 1443
PsrI GAACNNNNNNTAC 2 cut(s) 2215, 2247
PstI CTGCAG 5 cut(s) 1893, 1939, 2277, 2705, 3276
PstNI CAGNNNCTG 2 cut(s) 3895, 3926
PsuI RGATCY 3 cut(s) 1604, 2440, 4084
RsaI GTAC 9 cut(s) 2224, 2262, 2323, 2372, 2899, 2906, 3189, 3563, 3915
RsaNI GTAC 9 cut(s) 2223, 2261, 2322, 2371, 2898, 2905, 3188, 3562, 3914
RseI CAYNNNNRTG 6 cut(s) 528, 1461, 2193, 2363, 2382, 4239
SapI GCTCTTC 1 cut(s) 1848
Sau96I GGNCC 2 cut(s) 1443, 4266
ScaI AGTACT 3 cut(s) 2224, 3563, 3915
SchI GAGTC 7 cut(s) 179, 392, 493, 764, 906, 3547, 4071
ScrFI CCNGG 7 cut(s) 182, 700, 921, 1272, 2192, 3237, 4011
SduI GDGCHC 3 cut(s) 19, 919, 3364
SfcI CTRYAG 6 cut(s) 1889, 1935, 2273, 2701, 2754, 3272
Sfr274I CTCGAG 1 cut(s) 3407
SfuI TTCGAA 2 cut(s) 1032, 3786
SinI GGWCC 2 cut(s) 1443, 4266
SlaI CTCGAG 1 cut(s) 3407
SmiMI CAYNNNNRTG 6 cut(s) 528, 1461, 2193, 2363, 2382, 4239
SpeI ACTAGT 1 cut(s) 3620
SsiI CCGC 4 cut(s) 465, 1753, 1770, 3349
SspI AATATT 3 cut(s) 1975, 3074, 3197
StyD4I CCNGG 7 cut(s) 180, 698, 919, 1270, 2190, 3235, 4009
StyI CCWWGG 4 cut(s) 2064, 2416, 2879, 3705
TaiI ACGT 3 cut(s) 216, 379, 720
TaqII GACCGA 1 cut(s) 3102
TatI WGTACW 5 cut(s) 2222, 2897, 3187, 3561, 3913
TscAI CASTG 9 cut(s) 1018, 1468, 1645, 2152, 2277, 2477, 2803, 3525, 3962
TseFI GTSAC 5 cut(s) 1463, 1470, 2097, 3700, 3775
Tsp45I GTSAC 5 cut(s) 1463, 1470, 2097, 3700, 3775
TspGWI ACGGA 1 cut(s) 2168
TspRI CASTG 9 cut(s) 1018, 1468, 1645, 2152, 2277, 2477, 2803, 3525, 3962
Van91I CCANNNNNTGG 2 cut(s) 2417, 3749
Vha464I CTTAAG 1 cut(s) 3907
VneI GTGCAC 1 cut(s) 915
VpaK11BI GGWCC 2 cut(s) 1443, 4266
VspI ATTAAT 2 cut(s) 1343, 1898
XagI CCTNNNNNAGG 2 cut(s) 3377, 3731
XbaI TCTAGA 4 cut(s) 1060, 2677, 3982, 4228
XcmI CCANNNNNNNNNTGG 1 cut(s) 1268
XhoI CTCGAG 1 cut(s) 3407
XmiI GTMKAC 1 cut(s) 1571
XmnI GAANNNNTTC 2 cut(s) 2811, 3303
ZraI GACGTC 1 cut(s) 214
ZrmI AGTACT 3 cut(s) 2224, 3563, 3915
Zsp2I ATGCAT 2 cut(s) 1488, 2463
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.