Rroxscaffold_4G00328790

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0004242
Physical Location & Seq
Reverse (-)
61866421 .. 61870746
4326 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00328790.1

Sequence Viewer

Length: 4326 bp
ATGCCTATTGTTCGAGCACTTGAATCTGCTTCTGTAAGCATCCTCCTTAACAAGTTGGCCACTCAAGATGTCATAGATTTCTTCCTCAAGTGGAAAATTGATGACTCCCTGCGAGAAAAGCTCAAGAGAAACCTGCTTGTCATTCATGCAGTTCTCAATCATGCTGAGGAGAAGCAAGTCCGGGACTCAAATGTCAAAGCCTGGCTCGAGGACGTCAGAGTTGCAGCTTATGATGCCGAGGACATATTGGATGACATCGCCAATGATGCTCTCGAATCACAAAACAGCAAACATGAGGTATGGAATTCTAACATCAAAGAAGGGATAGACTTTAAGATCAAAGACATTGCTAATACTCTTAATCCTTTTAGAGAACGTATAGAGTCGAAAATGATAAAGATTATAGATGTATTGGAAGAGATTGCAAAGCAGAAAGATATTCTTCGTTTGAGAGAAGATTCTGGCGGTATAGAATCAGGTTTTGACGGATTGCCCACGACTCCAATGTTGGGTGATAAATCTCATGTTTATGGTAGAGAATCTGATAAGAAGGAGATAATTGCTTTGCTGAAATCGGGTAAAAACGATCGTGAGGTTTCTATAATTCCAATTGTAGGCATGGGAGGCATTGGGAAGACTACTCTTGCTCAACTTGTGTATAATGATGCTAAAGTGAGTAGCTATTTTAATTTGAGAGCCTGGGCTTGTGTCTCTGATGTATTTGATGTGCAAAGGATAACAAAAACACTTGTTGAGTCCGCGACAAAATCAAGTTGTAGCACAAACAATTTGGAGTTACTTCAAGAACAGCTGAAAATACGGTTGAAGAACAAGAAATTTCTTTTTGTTCTGGATGATGTCTGGAACGAGAAGTATGAAAGCTGGGATAAGTTAAGAGTCCCCTTCACAGTTGGTGCACCAGGGAGTAAAATCATAATCACGACACGAAACAAAACAGTTGCATCACTTATGGGGACTGTTCCTACTTACTGTTTGGAGGGGCTTTCGGACAGTGATTGTTGGTTATTATTCGAACAAATTGTGTTTCAGAACAGGAATCTAGATGCCTATCCAAAGTTGAAAGTGATTGGCAAGAAAATAGTGGGCAAGTGTAAAGGGCTGCCTTTGGCTGCAAAGGCACTAGGAGGTCTCCTGCGTGCTGAACCGGAACTAGATGAGAATTACTGGAATGATATCTTGAATAGCAAACTTTGGAAACTGGCAGACAACACTATTCTTCCAGCTCTAAGATTAAGCTACCATCACCTCCCTGGGCATTTGAAGCGCTGCTTTGCTTATTGCTCGATGTTTCCTAAGGACTATGAATATGAGAGAGAGATGTTAATCTTGTTATGGATGGCAGAGGGTTTTGTGCCAGAGCCAGAAGGAAATCAACGAATCGAAGATGTAGCTGGAAGATATGTTTCAGATTTTTTATCAAGGTCCTTCTTCCAACACACCAGTGACAAGTCACGACTGGTAATGCATGACCTCATCCATGATCTTGCACAATCTGTTTCTGGCAAAACATTTGTTAGGTTGGAGGAAAATTCAGAGAACCATAAAAAGTCTACAAAGGCTCGGCACTTGTCATATACCCGTGGATCTGAAGATGTATTTCAGAAATTTGAGGCATTCAGTGAGGTGGATTGTTTACGAACCTTTCTACCACTAGACCCGTTGCAAGGATTCAATGTGAGTGGCTTATCTGACAAGGTTCCTTGTGATATGATGCCAAGATTAAGACTCCTGCGGGTGCTATCCTTTAGTGGTTATCTCATAGAAAAGTTGCCAGATTCAATTGGCAACTTGAAGCATCTGCGATACCTCAATCTTTCTTATTGTCAAATAGAAGAGCTACCTGAGCCAACAAGCAGTCTATACAATCTGCAGACATTAATTTTATTCAGATGTCAATCGCTTACCATGCTGCCTGCAGACATAGGAAACCTAAGGAATTTACGGCATCTCAATATTCAGGGAACTAGGCTGAAAACTATGCCTTTGGGAATGGGAAGATTGGTTAACCTTCAAATACTGTCAGATTTTATTGTGGGGAAAGCCATGGGAAAAGGGATTGCAGAATTGAAGGACTTGTCACATCTTCGAGGCTCAGTTTCTATTTTGTGTTTGCATGATGTCAGCAGTGTTACGGATGCAATTAAGGCCAGATTAGAGGTTAAGAAGCACCTTGATGATTTAGTTTTGGAATGGAGCAGCAGTACTGATGGTTTGCGAAATGAAGAGATCGAGACAGAGGTACTTGATGCACTGCAGCCTCATGAAAACCTGAAGAAACTCACCATTAAGTATTATGGAGGTACAGAATTTCCATATTGGATGGGGAATCCTTTGTTCACTAACATGGCGTACTTGCATCTCTATGGTTGCATAAAATGCACATCTTTACCACCCCTTGGCCAACTACCTTCTCTTAAAGATCTCATAATTGAAGCAATGCATGGAATACAGTGTGTAGGAATGGATTTTTTTGGGGATGATGATGGTTCCACAATTTTTTTCCCCTATTTAGAGACTTTGAAATTTGAGGACATGAAAGAATGGGAGAAATGGTCATCTTCAGAAGATGAAAGGCTTAAAGGATTTCCCAGCCTTCGGGAGCTGTCCATATTTAGATGTCCCAAGTTAACAAAGTTTACACATGGATTTGCTTCTCTAGAAAAGCTGCGCATCAAGAACTGCAGAGCAATAACTACCTTCTCACAGAATCCTGCACTTGGGAAATTGGAACCTGTAGAATTCCCTAGCCTGAATTCGGAACCTGTAGAATTCCCTAGCCTGCAACTACTTGTTCTTGTTGGTTGCAGTGAGCTTGAAAACCTTCCTTTTTCCCTCCCTTTGTTGAAAATGCTTGAGATAGATGGTTGTGAAAAATTGGCTGCACTTCCGAGGATGGTGGAACTTAGTACATTGTACCTGATCGATTCAAATGCTGAATTACTTGGATCCAAGACGGAATTTAGCTCGCTGACCTCTTTGCACCTAACTCATATTCCAGATGTGAAGCATCTACCTGAAGGCTTTATCCAACAGTCTGCAAAACTTGAAAAATTGAGTCTTACAAGCTTTCCTGACCTTGAATATTTGGCAGATGACCGAGTTGGACTAGCACACCTTGTATCCCTTCAACGCTTGACAATATCCCATTGTCCTAAGTTTGTTGCTTTGCCTGATGAGCTCCCACCTGTACTCAAATATTTGGATTTGAAACATTGTCCTAGCCTGATGAAGCTGCCTGGTAAACTTCACAACCTTGAGTGTCTTGCAGAGCTGCAGATTGAATGGTGTTCAAAGATTGAAGCATTTCCAGATGTGGGGTTGCCTGACAAACTGAAGCGTCTTGTAATCCGCGAATGTGGTGCTCTGAAGACTCTTCCTCAGGAGCTTCTCTGCAATAACAATTTTCTCGAGTATTTAGAAGTTCATAAATGTCCTTCTCTTGTGTCAGTTCTTGAAGAAGGAAACATGCCATCAACTCTAACAAATATGAAATTTTGCTATTGCAAAATTCTGGGATCACTGCCTGAGGGTTTGATGCACAAAGACAATATGACTCTAAAGTACTTGGAGATAGACAACTGTTCCTCTCTCATGTCCTTTCCCAATGGTGAGTTACCAACAAGTCTTGAGCGTCTTGAGATTAGTGATTGCTCTAATTTCCAGACATTGCCATTGAGCCTTCTCAACCTCACGAATCTTGAGACCTTGGAAGTAAAGAGCTGCCCCCTTCTTCAGGACTTTCCAGAGGGTGGATTGCCACCCAACATAAAGTTTGTCACAATTTCCGAATGTGAGAATCTCAAGTCTCTACCTAAATTCTTTCATAAACTCAAATGTCTTCAGAAGTTGGAGATTTCTGGCTGTCCTAGTCTCCTGTCCTTGCCAAAGCAGGGTCTGCCCAGCAGCTTAAGACTACTTACAGTTACTGATTGTGAAAAGCTTAATCCCATACATCAGTGGAGACTTCACAAACTACCCTCTCTAGATGTTTTCACCATTGGTGGATTTCCTGGCCTGAAGTCCTTTTCAAAAGATTATCCTCTTCCTCACACTTTAACCTCTTTAAGCATTCAAAGATTCCCAGATCTTGAATCCATATCGGAGGTGCTTGAGAATCTCACCTCACTTGAAAACCTGAGTATCAGGGAGTGTGATAAGCTCCAATCTTTGCCAGAGACGGGGCTACCAGCGACAATTTCTACCTTGACAATTCGGAATTGTTTGCTTCTAGAACCTCGATGTGAAAAGGACAAAGGAGAAGATTGGTTCAAGATCAAAAACATCCCCTTCGTGAGTTCACGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

1441

Amino Acids

162.99

Weight (kDa)

5.98

Isoelectric Point (pI)

45.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 12 - 98 9.7e-17 Rx N-terminal domain
NB-ARC PF00931 181 - 352 6.8e-36 NB-ARC domain
WHD_DRP PF23559 436 - 503 1.1e-24 Disease resistance protein Winged helix domain
LRR_14 PF23598 578 - 850 2.6e-30 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 693 - 820 1.3e-39 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 191
AatII GACGTC 1 cut(s) 216
Acc16I TGCGCA 1 cut(s) 2690
Acc36I ACCTGC 1 cut(s) 141
AccB7I CCANNNNNTGG 2 cut(s) 2417, 3770
AccI GTMKAC 1 cut(s) 1571
AccII CGCG 2 cut(s) 761, 3372
AciI CCGC 4 cut(s) 465, 759, 1753, 3370
AclWI GGATC 4 cut(s) 1612, 2961, 2974, 3544
AcoI YGGCCR 2 cut(s) 57, 2419
AcuI CTGAAG 9 cut(s) 1629, 2312, 2565, 3057, 3374, 3407, 3737, 3845, 4058
AcyI GRCGYC 1 cut(s) 213
AfaI GTAC 8 cut(s) 2224, 2262, 2323, 2372, 2929, 2936, 3210, 3584
AfeI AGCGCT 1 cut(s) 1286
AflII CTTAAG 1 cut(s) 3928
AjnI CCWGG 6 cut(s) 200, 698, 919, 1270, 3256, 4030
AleI CACNNNNGTG 1 cut(s) 1461
Alw21I GWGCWC 4 cut(s) 19, 919, 3201, 3385
Alw26I GTCTC 9 cut(s) 715, 1154, 2246, 2528, 3716, 3831, 3896, 3976, 4190
Alw44I GTGCAC 1 cut(s) 915
AlwI GGATC 4 cut(s) 1612, 2961, 2974, 3544
AlwNI CAGNNNCTG 2 cut(s) 3916, 3947
Ama87I CYCGRG 2 cut(s) 206, 3428
Aor51HI AGCGCT 1 cut(s) 1286
AoxI GGCC 4 cut(s) 57, 2166, 2419, 4033
ApaLI GTGCAC 1 cut(s) 915
AseI ATTAAT 1 cut(s) 1898
Asp700I GAANNNNTTC 2 cut(s) 2841, 3324
AspLEI GCGC 2 cut(s) 1287, 2691
AspS9I GGNCC 1 cut(s) 1443
AsuC2I CCSGG 1 cut(s) 182
AsuHPI GGTGA 6 cut(s) 524, 1256, 2293, 3641, 4006, 4132
AsuII TTCGAA 1 cut(s) 1032
AvaI CYCGRG 2 cut(s) 206, 3428
AvaII GGWCC 1 cut(s) 1443
AxyI CCTNAGG 4 cut(s) 1314, 1952, 3399, 3546
BaeGI GKGCMC 1 cut(s) 919
BaeI ACNNNNGTAYC 2 cut(s) 4144, 4177
BalI TGGCCA 2 cut(s) 59, 2421
BamHI GGATCC 1 cut(s) 2966
BanII GRGCYC 1 cut(s) 3201
BbsI GAAGAC 3 cut(s) 641, 3395, 3851
Bbv12I GWGCWC 4 cut(s) 19, 919, 3201, 3385
BbvCI CCTCAGC 1 cut(s) 165
BccI CCATC 8 cut(s) 1269, 1351, 2222, 2335, 2498, 2876, 2908, 3499
BceAI ACGGC 1 cut(s) 1979
BcgI CGANNNNNNTGC 4 cut(s) 2933, 2967, 3362, 3396
BciT130I CCWGG 6 cut(s) 202, 700, 921, 1272, 3258, 4032
BciVI GTATCC 1 cut(s) 3151
BcnI CCSGG 1 cut(s) 182
BcoDI GTCTC 9 cut(s) 715, 1154, 2246, 2528, 3716, 3831, 3896, 3976, 4190
BfmI CTRYAG 7 cut(s) 1889, 1935, 2273, 2701, 2754, 2784, 3293
BfoI RGCGCY 1 cut(s) 1288
BfrI CTTAAG 1 cut(s) 3928
BfuAI ACCTGC 1 cut(s) 141
BfuI GTATCC 1 cut(s) 3151
BglII AGATCT 2 cut(s) 2440, 4105
BmcAI AGTACT 2 cut(s) 2224, 3584
Bme1390I CCNGG 7 cut(s) 182, 202, 700, 921, 1272, 3258, 4032
Bme18I GGWCC 1 cut(s) 1443
BmeT110I CYCGRG 2 cut(s) 206, 3428
BmgT120I GGNCC 1 cut(s) 1443
BmiI GGNNCC 5 cut(s) 1719, 2509, 2751, 2781, 2968
BmrFI CCNGG 7 cut(s) 182, 202, 700, 921, 1272, 3258, 4032
BpiI GAAGAC 3 cut(s) 641, 3395, 3851
BplI GAGNNNNNCTC 2 cut(s) 105, 137
Bpu10I CCTNAGC 2 cut(s) 165, 1863
Bpu14I TTCGAA 1 cut(s) 1032
BpuMI CCSGG 1 cut(s) 182
Bsa29I ATCGAT 1 cut(s) 2943
BsaBI GATNNNNATC 3 cut(s) 1068, 1343, 1915
BsaHI GRCGYC 1 cut(s) 213
BsaI GGTCTC 2 cut(s) 1154, 3716
BsaWI WCCGGW 1 cut(s) 1165
BsaXI ACNNNNNCTCC 4 cut(s) 2206, 2236, 2558, 2588
Bse1I ACTGG 4 cut(s) 1190, 1224, 1461, 1482
Bse21I CCTNAGG 4 cut(s) 1314, 1952, 3399, 3546
Bse3DI GCAATG 3 cut(s) 345, 2463, 3686
Bse8I GATNNNNATC 3 cut(s) 1068, 1343, 1915
BseBI CCWGG 6 cut(s) 202, 700, 921, 1272, 3258, 4032
BseCI ATCGAT 1 cut(s) 2943
BseJI GATNNNNATC 3 cut(s) 1068, 1343, 1915
BseMI GCAATG 3 cut(s) 345, 2463, 3686
BseMII CTCAG 6 cut(s) 156, 1854, 2127, 3413, 3537, 4148
BseNI ACTGG 4 cut(s) 1190, 1224, 1461, 1482
BseRI GAGGAG 1 cut(s) 182
BseSI GKGCMC 1 cut(s) 919
BseYI CCCAGC 3 cut(s) 882, 2609, 3920
BsgI GTGCAG 2 cut(s) 2718, 2886
Bsh1236I CGCG 2 cut(s) 761, 3372
Bsh1285I CGRYCG 1 cut(s) 589
BshFI GGCC 4 cut(s) 59, 2168, 2421, 4035
BshVI ATCGAT 1 cut(s) 2943
BsiEI CGRYCG 1 cut(s) 589
BsiHKAI GWGCWC 4 cut(s) 19, 919, 3201, 3385
BsiHKCI CYCGRG 2 cut(s) 206, 3428
BsiSI CCGG 2 cut(s) 181, 1166
BslFI GGGAC 4 cut(s) 197, 884, 988, 2625
BsmAI GTCTC 9 cut(s) 715, 1154, 2246, 2528, 3716, 3831, 3896, 3976, 4190
BsmBI CGTCTC 1 cut(s) 4190
BsmFI GGGAC 4 cut(s) 197, 884, 988, 2625
BsmI GAATGC 2 cut(s) 1634, 4089
BsnI GGCC 4 cut(s) 59, 2168, 2421, 4035
Bso31I GGTCTC 2 cut(s) 1154, 3716
BsoBI CYCGRG 2 cut(s) 206, 3428
Bsp119I TTCGAA 1 cut(s) 1032
Bsp1286I GDGCHC 4 cut(s) 19, 919, 3201, 3385
Bsp19I CCATGG 1 cut(s) 2064
BspACI CCGC 4 cut(s) 465, 759, 1753, 3370
BspANI GGCC 4 cut(s) 59, 2168, 2421, 4035
BspCNI CTCAG 6 cut(s) 157, 1855, 2126, 3412, 3538, 4149
BspDI ATCGAT 1 cut(s) 2943
BspFNI CGCG 2 cut(s) 761, 3372
BspHI TCATGA 1 cut(s) 2281
BspLI GGNNCC 5 cut(s) 1719, 2509, 2751, 2781, 2968
BspMAI CTGCAG 5 cut(s) 1893, 1939, 2277, 2705, 3297
BspMI ACCTGC 1 cut(s) 141
BspPI GGATC 4 cut(s) 1612, 2961, 2974, 3544
BspQI GCTCTTC 1 cut(s) 1848
BspT104I TTCGAA 1 cut(s) 1032
BspTI CTTAAG 1 cut(s) 3928
BspTNI GGTCTC 2 cut(s) 1154, 3716
BsrDI GCAATG 3 cut(s) 345, 2463, 3686
BsrI ACTGG 4 cut(s) 1190, 1224, 1461, 1482
BssNI GRCGYC 1 cut(s) 213
BssT1I CCWWGG 3 cut(s) 2064, 2416, 3726
Bst2UI CCWGG 6 cut(s) 202, 700, 921, 1272, 3258, 4032
Bst6I CTCTTC 5 cut(s) 411, 1848, 2238, 3399, 4068
BstACI GRCGYC 1 cut(s) 213
BstAFI CTTAAG 1 cut(s) 3928
BstAPI GCANNNNNTGC 2 cut(s) 2397, 3916
BstBI TTCGAA 1 cut(s) 1032
BstC8I GCNNGC 4 cut(s) 1158, 1935, 2801, 2987
BstDSI CCRYGG 2 cut(s) 1600, 2064
BstENI CCTNNNNNAGG 1 cut(s) 3752
BstFNI CGCG 2 cut(s) 761, 3372
BstH2I RGCGCY 1 cut(s) 1288
BstHHI GCGC 2 cut(s) 1287, 2691
BstMAI GTCTC 9 cut(s) 715, 1154, 2246, 2528, 3716, 3831, 3896, 3976, 4190
BstMCI CGRYCG 1 cut(s) 589
BstNI CCWGG 6 cut(s) 202, 700, 921, 1272, 3258, 4032
BstNSI RCATGY 1 cut(s) 3490
BstSCI CCNGG 7 cut(s) 180, 200, 698, 919, 1270, 3256, 4030
BstSFI CTRYAG 7 cut(s) 1889, 1935, 2273, 2701, 2754, 2784, 3293
BstSLI GKGCMC 1 cut(s) 919
BstUI CGCG 2 cut(s) 761, 3372
BstV2I GAAGAC 3 cut(s) 641, 3395, 3851
BstX2I RGATCY 4 cut(s) 1604, 2440, 2966, 4105
BstYI RGATCY 4 cut(s) 1604, 2440, 2966, 4105
Bsu15I ATCGAT 1 cut(s) 2943
Bsu36I CCTNAGG 4 cut(s) 1314, 1952, 3399, 3546
BsuI GTATCC 1 cut(s) 3151
BsuRI GGCC 4 cut(s) 59, 2168, 2421, 4035
BsuTUI ATCGAT 1 cut(s) 2943
BtgI CCRYGG 2 cut(s) 1600, 2064
BtgZI GCGATG 1 cut(s) 241
BtsI GCAGTG 4 cut(s) 2152, 2270, 2833, 3539
BtsIMutI CAGTG 9 cut(s) 1018, 1468, 1645, 2152, 2270, 2477, 2833, 3539, 3983
BveI ACCTGC 1 cut(s) 141
Cac8I GCNNGC 4 cut(s) 1158, 1935, 2801, 2987
CaiI CAGNNNCTG 2 cut(s) 3916, 3947
CciI TCATGA 1 cut(s) 2281
CfoI GCGC 2 cut(s) 1287, 2691
Cfr13I GGNCC 1 cut(s) 1443
ClaI ATCGAT 1 cut(s) 2943
CseI GACGC 2 cut(s) 3347, 3641
Csp6I GTAC 8 cut(s) 2223, 2261, 2322, 2371, 2928, 2935, 3209, 3583
CspCI CAANNNNNGTGG 2 cut(s) 1681, 1716
CviQI GTAC 8 cut(s) 2223, 2261, 2322, 2371, 2928, 2935, 3209, 3583
DrdI GACNNNNNNGTC 1 cut(s) 191
DseDI GACNNNNNNGTC 1 cut(s) 191
EaeI YGGCCR 2 cut(s) 57, 2419
Eam1104I CTCTTC 5 cut(s) 411, 1848, 2238, 3399, 4068
EarI CTCTTC 5 cut(s) 411, 1848, 2238, 3399, 4068
Ecl136II GAGCTC 1 cut(s) 3199
Eco130I CCWWGG 3 cut(s) 2064, 2416, 3726
Eco24I GRGCYC 1 cut(s) 3201
Eco31I GGTCTC 2 cut(s) 1154, 3716
Eco32I GATATC 1 cut(s) 1195
Eco47I GGWCC 1 cut(s) 1443
Eco47III AGCGCT 1 cut(s) 1286
Eco53kI GAGCTC 1 cut(s) 3199
Eco57I CTGAAG 9 cut(s) 1629, 2312, 2565, 3057, 3374, 3407, 3737, 3845, 4058
Eco81I CCTNAGG 4 cut(s) 1314, 1952, 3399, 3546
Eco88I CYCGRG 2 cut(s) 206, 3428
EcoICRI GAGCTC 1 cut(s) 3199
EcoNI CCTNNNNNAGG 1 cut(s) 3752
EcoO109I RGGNCCY 1 cut(s) 1443
EcoRI GAATTC 4 cut(s) 304, 2759, 2773, 2789
EcoRII CCWGG 6 cut(s) 200, 698, 919, 1270, 3256, 4030
EcoRV GATATC 1 cut(s) 1195
EcoT14I CCWWGG 3 cut(s) 2064, 2416, 3726
EcoT22I ATGCAT 2 cut(s) 1488, 2463
EcoT38I GRGCYC 1 cut(s) 3201
ErhI CCWWGG 3 cut(s) 2064, 2416, 3726
Esp3I CGTCTC 1 cut(s) 4190
FalI AAGNNNNNCTT 4 cut(s) 426, 458, 1274, 1306
FaqI GGGAC 4 cut(s) 197, 884, 988, 2625
FauI CCCGC 1 cut(s) 1746
FblI GTMKAC 1 cut(s) 1571
FriOI GRGCYC 1 cut(s) 3201
FspI TGCGCA 1 cut(s) 2690
GlaI GCGC 2 cut(s) 1286, 2690
GsaI CCCAGC 3 cut(s) 886, 2613, 3924
HaeII RGCGCY 1 cut(s) 1288
HaeIII GGCC 4 cut(s) 59, 2168, 2421, 4035
HapII CCGG 2 cut(s) 181, 1166
HgaI GACGC 2 cut(s) 3347, 3641
HhaI GCGC 2 cut(s) 1287, 2691
Hin1I GRCGYC 1 cut(s) 213
Hin6I GCGC 2 cut(s) 1285, 2689
HinP1I GCGC 2 cut(s) 1285, 2689
HincII GTYRAC 2 cut(s) 2026, 2649
HindII GTYRAC 2 cut(s) 2026, 2649
HindIII AAGCTT 2 cut(s) 3085, 3959
HpaI GTTAAC 2 cut(s) 2026, 2649
HpaII CCGG 2 cut(s) 181, 1166
HphI GGTGA 6 cut(s) 524, 1256, 2293, 3641, 4006, 4132
Hpy166II GTNNAC 9 cut(s) 917, 1572, 1655, 2026, 2358, 2649, 2658, 3263, 4319
Hpy8I GTNNAC 9 cut(s) 917, 1572, 1655, 2026, 2358, 2649, 2658, 3263, 4319
HpyCH4IV ACGT 2 cut(s) 213, 376
HpySE526I ACGT 2 cut(s) 213, 376
Hsp92I GRCGYC 1 cut(s) 213
HspAI GCGC 2 cut(s) 1285, 2689
KspAI GTTAAC 2 cut(s) 2026, 2649
LguI GCTCTTC 1 cut(s) 1848
LmnI GCTCC 5 cut(s) 2214, 2620, 3204, 3403, 4185
MaeII ACGT 2 cut(s) 213, 376
MaeIII GTNAC 8 cut(s) 795, 1463, 1470, 2097, 2149, 3633, 3796, 3943
MfeI CAATTG 2 cut(s) 609, 1800
MflI RGATCY 4 cut(s) 1604, 2440, 2966, 4105
MhlI GDGCHC 4 cut(s) 19, 919, 3201, 3385
MlsI TGGCCA 2 cut(s) 59, 2421
MluNI TGGCCA 2 cut(s) 59, 2421
MmeI TCCRAC 5 cut(s) 1477, 1521, 3073, 3103, 3849
Mox20I TGGCCA 2 cut(s) 59, 2421
Mph1103I ATGCAT 2 cut(s) 1488, 2463
MroXI GAANNNNTTC 2 cut(s) 2841, 3324
MscI TGGCCA 2 cut(s) 59, 2421
MslI CAYNNNNRTG 5 cut(s) 528, 1461, 2193, 2363, 2382
Msp20I TGGCCA 2 cut(s) 59, 2421
MspA1I CMGCKG 1 cut(s) 811
MspCI CTTAAG 1 cut(s) 3928
MspI CCGG 2 cut(s) 181, 1166
MspR9I CCNGG 7 cut(s) 182, 202, 700, 921, 1272, 3258, 4032
MunI CAATTG 2 cut(s) 609, 1800
Mva1269I GAATGC 2 cut(s) 1634, 4089
MvaI CCWGG 6 cut(s) 202, 700, 921, 1272, 3258, 4032
MvnI CGCG 2 cut(s) 761, 3372
NciI CCSGG 1 cut(s) 182
NcoI CCATGG 1 cut(s) 2064
NlaIV GGNNCC 5 cut(s) 1719, 2509, 2751, 2781, 2968
NmeAIII GCCGAG 2 cut(s) 262, 1561
NmuCI GTSAC 4 cut(s) 1463, 1470, 2097, 3796
NsbI TGCGCA 1 cut(s) 2690
NsiI ATGCAT 2 cut(s) 1488, 2463
NspI RCATGY 1 cut(s) 3490
NspV TTCGAA 1 cut(s) 1032
OliI CACNNNNGTG 1 cut(s) 1461
PaeR7I CTCGAG 2 cut(s) 206, 3428
PagI TCATGA 1 cut(s) 2281
PasI CCCWGGG 1 cut(s) 1271
PciSI GCTCTTC 1 cut(s) 1848
PctI GAATGC 2 cut(s) 1634, 4089
PdmI GAANNNNTTC 2 cut(s) 2841, 3324
PflMI CCANNNNNTGG 2 cut(s) 2417, 3770
PfoI TCCNGGA 1 cut(s) 180
Ple19I CGATCG 1 cut(s) 589
PpuMI RGGWCCY 1 cut(s) 1443
PshBI ATTAAT 1 cut(s) 1898
Psp124BI GAGCTC 1 cut(s) 3201
Psp5II RGGWCCY 1 cut(s) 1443
Psp6I CCWGG 6 cut(s) 200, 698, 919, 1270, 3256, 4030
PspFI CCCAGC 3 cut(s) 882, 2609, 3920
PspGI CCWGG 6 cut(s) 200, 698, 919, 1270, 3256, 4030
PspN4I GGNNCC 5 cut(s) 1719, 2509, 2751, 2781, 2968
PspPI GGNCC 1 cut(s) 1443
PspPPI RGGWCCY 1 cut(s) 1443
PspXI VCTCGAGB 1 cut(s) 206
PstI CTGCAG 5 cut(s) 1893, 1939, 2277, 2705, 3297
PstNI CAGNNNCTG 2 cut(s) 3916, 3947
PsuI RGATCY 4 cut(s) 1604, 2440, 2966, 4105
PvuI CGATCG 1 cut(s) 589
PvuII CAGCTG 1 cut(s) 811
RsaI GTAC 8 cut(s) 2224, 2262, 2323, 2372, 2929, 2936, 3210, 3584
RsaNI GTAC 8 cut(s) 2223, 2261, 2322, 2371, 2928, 2935, 3209, 3583
RseI CAYNNNNRTG 5 cut(s) 528, 1461, 2193, 2363, 2382
SacI GAGCTC 1 cut(s) 3201
SapI GCTCTTC 1 cut(s) 1848
Sau96I GGNCC 1 cut(s) 1443
ScaI AGTACT 2 cut(s) 2224, 3584
ScrFI CCNGG 7 cut(s) 182, 202, 700, 921, 1272, 3258, 4032
SduI GDGCHC 4 cut(s) 19, 919, 3201, 3385
SfcI CTRYAG 7 cut(s) 1889, 1935, 2273, 2701, 2754, 2784, 3293
Sfr274I CTCGAG 2 cut(s) 206, 3428
SfuI TTCGAA 1 cut(s) 1032
SinI GGWCC 1 cut(s) 1443
SlaI CTCGAG 2 cut(s) 206, 3428
SmiMI CAYNNNNRTG 5 cut(s) 528, 1461, 2193, 2363, 2382
SsiI CCGC 4 cut(s) 465, 759, 1753, 3370
SspI AATATT 3 cut(s) 1975, 3104, 3218
SstI GAGCTC 1 cut(s) 3201
StyD4I CCNGG 7 cut(s) 180, 200, 698, 919, 1270, 3256, 4030
StyI CCWWGG 3 cut(s) 2064, 2416, 3726
TaiI ACGT 2 cut(s) 216, 379
TaqII GACCGA 1 cut(s) 3132
TatI WGTACW 4 cut(s) 2222, 2927, 3208, 3582
TscAI CASTG 9 cut(s) 1018, 1468, 1645, 2152, 2277, 2477, 2833, 3546, 3983
TseFI GTSAC 4 cut(s) 1463, 1470, 2097, 3796
Tsp45I GTSAC 4 cut(s) 1463, 1470, 2097, 3796
TspGWI ACGGA 3 cut(s) 501, 2168, 2990
TspRI CASTG 9 cut(s) 1018, 1468, 1645, 2152, 2277, 2477, 2833, 3546, 3983
Van91I CCANNNNNTGG 2 cut(s) 2417, 3770
Vha464I CTTAAG 1 cut(s) 3928
VneI GTGCAC 1 cut(s) 915
VpaK11BI GGWCC 1 cut(s) 1443
VspI ATTAAT 1 cut(s) 1898
XagI CCTNNNNNAGG 1 cut(s) 3752
XbaI TCTAGA 4 cut(s) 1060, 2677, 4003, 4249
XceI RCATGY 1 cut(s) 3490
XcmI CCANNNNNNNNNTGG 1 cut(s) 1268
XhoI CTCGAG 2 cut(s) 206, 3428
XmiI GTMKAC 1 cut(s) 1571
XmnI GAANNNNTTC 2 cut(s) 2841, 3324
ZraI GACGTC 1 cut(s) 214
ZrmI AGTACT 2 cut(s) 2224, 3584
Zsp2I ATGCAT 2 cut(s) 1488, 2463
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.