RchiOBHm_Chr4g0403521

zf-MYND-like zinc finger, mRNA-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0003129
Physical Location & Seq
Forward (+)
22821520 .. 22823362
1843 bp
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UTR
Exon/CDS
Intron
PRQ37525

Sequence Viewer

Length: 540 bp
ATGGCCGACGTCGCCACCTTGTCGTGTGCTCATTGCAGCAAACCCGCCAATCTTCAGTGTCCCAAGTGTGTGGAATTGAAGCTTCCTCGTGAAGATGCCCTTTCTGGTTTCACCCAAGATTGTTTTAAGGCTTCGTGGAGCTTGCATAAATTGGTACATTTAAAGGCCAAGCAGTCCAATGGGGCGGGAAATCCAGAGAACGAAGGCTGGCAATATTGCATGAAGAAAGGACAGGGTCGAACTCCAAAACTTCCTTATTTTGATTGGACAGGGACATTGAGACCATATCCTATATCTAGTAAGCGTGTGGTACCTTCTCATATTGATCTACCTGATTGGGCAGCTGATATGTTGCATTGTGTTGGTACTTCTAGATGCAAATATGTCGTCATGGTTTTGACCTTTAAAAAATTGCAAGGAACCCCAAAAGAGGAACCCAGTAGTGACCTGCAGCGTGTTGTTGAGATCAAAACTCCGGATCAAATTGAAAGGATGCGAGAAACTTGTCGATGCTTTTTCACTTTATCCTGTGGACCTTAA

Protein Analysis

179

Amino Acids

20.15

Weight (kDa)

8.86

Isoelectric Point (pI)

40.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C6H2 PF15801 10 - 54 1.8e-10 zf-MYND-like zinc finger, mRNA-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 12
Acc36I ACCTGC 1 cut(s) 456
Acc65I GGTACC 1 cut(s) 310
AccB1I GGYRCC 1 cut(s) 310
AccIII TCCGGA 1 cut(s) 475
AciI CCGC 2 cut(s) 45, 185
AclWI GGATC 1 cut(s) 486
AcoI YGGCCR 1 cut(s) 3
AcuI CTGAAG 1 cut(s) 38
AcyI GRCGYC 1 cut(s) 9
AfaI GTAC 3 cut(s) 156, 312, 367
AfiI CCNNNNNNNGG 1 cut(s) 430
AgsI TTSAA 2 cut(s) 79, 488
AluBI AGCT 3 cut(s) 82, 141, 344
AluI AGCT 3 cut(s) 82, 141, 344
Alw21I GWGCWC 1 cut(s) 31
Alw26I GTCTC 1 cut(s) 274
AlwI GGATC 1 cut(s) 486
Aor13HI TCCGGA 1 cut(s) 475
AoxI GGCC 2 cut(s) 3, 165
ApeKI GCWGC 3 cut(s) 36, 341, 451
ArsI GACNNNNNNTTYG 2 cut(s) 372, 404
Asp718I GGTACC 1 cut(s) 310
AspS9I GGNCC 1 cut(s) 533
AsuHPI GGTGA 1 cut(s) 103
AvaII GGWCC 1 cut(s) 533
BanI GGYRCC 1 cut(s) 310
BauI CACGAG 1 cut(s) 87
Bbv12I GWGCWC 1 cut(s) 31
BbvI GCAGC 3 cut(s) 48, 353, 463
BcgI CGANNNNNNTGC 2 cut(s) 367, 401
BcoDI GTCTC 1 cut(s) 274
BfaI CTAG 2 cut(s) 297, 372
BfmI CTRYAG 1 cut(s) 449
BfuAI ACCTGC 1 cut(s) 456
BisI GCNGC 3 cut(s) 37, 342, 452
BlsI GCNGC 3 cut(s) 38, 343, 453
Bme18I GGWCC 1 cut(s) 533
BmgT120I GGNCC 1 cut(s) 533
BmiI GGNNCC 3 cut(s) 312, 421, 435
BmrI ACTGGG 1 cut(s) 432
BmsI GCATC 4 cut(s) 85, 365, 483, 500
BmuI ACTGGG 1 cut(s) 432
BsaHI GRCGYC 1 cut(s) 9
BsaI GGTCTC 1 cut(s) 274
BsaWI WCCGGW 1 cut(s) 475
Bsc4I CCNNNNNNNGG 1 cut(s) 430
Bse1I ACTGG 1 cut(s) 438
Bse3DI GCAATG 1 cut(s) 31
BseAI TCCGGA 1 cut(s) 475
BseGI GGATG 1 cut(s) 498
BseLI CCNNNNNNNGG 1 cut(s) 430
BseMI GCAATG 1 cut(s) 31
BseNI ACTGG 1 cut(s) 438
BseXI GCAGC 3 cut(s) 48, 353, 463
BshFI GGCC 2 cut(s) 5, 167
BshNI GGYRCC 1 cut(s) 310
BsiHKAI GWGCWC 1 cut(s) 31
BsiSI CCGG 1 cut(s) 476
BslFI GGGAC 2 cut(s) 45, 286
BslI CCNNNNNNNGG 1 cut(s) 430
BsmAI GTCTC 1 cut(s) 274
BsmFI GGGAC 2 cut(s) 45, 286
BsnI GGCC 2 cut(s) 5, 167
Bso31I GGTCTC 1 cut(s) 274
Bsp1286I GDGCHC 1 cut(s) 31
Bsp13I TCCGGA 1 cut(s) 475
Bsp143I GATC 3 cut(s) 325, 465, 478
BspACI CCGC 2 cut(s) 45, 185
BspANI GGCC 2 cut(s) 5, 167
BspEI TCCGGA 1 cut(s) 475
BspLI GGNNCC 3 cut(s) 312, 421, 435
BspMAI CTGCAG 1 cut(s) 453
BspMI ACCTGC 1 cut(s) 456
BspPI GGATC 1 cut(s) 486
BspT107I GGYRCC 1 cut(s) 310
BspTNI GGTCTC 1 cut(s) 274
BsrDI GCAATG 1 cut(s) 31
BsrI ACTGG 1 cut(s) 438
BssMI GATC 3 cut(s) 325, 465, 478
BssNI GRCGYC 1 cut(s) 9
BssSI CACGAG 1 cut(s) 87
Bst2BI CACGAG 1 cut(s) 87
BstACI GRCGYC 1 cut(s) 9
BstC8I GCNNGC 2 cut(s) 143, 209
BstF5I GGATG 1 cut(s) 498
BstKTI GATC 3 cut(s) 328, 468, 481
BstMAI GTCTC 1 cut(s) 274
BstMBI GATC 3 cut(s) 325, 465, 478
BstMWI GCNNNNNNNGC 1 cut(s) 11
BstSFI CTRYAG 1 cut(s) 449
BstV1I GCAGC 3 cut(s) 48, 353, 463
BstXI CCANNNNNNTGG 1 cut(s) 70
BsuRI GGCC 2 cut(s) 5, 167
BtsCI GGATG 1 cut(s) 498
BtsIMutI CAGTG 1 cut(s) 62
BveI ACCTGC 1 cut(s) 456
Cac8I GCNNGC 2 cut(s) 143, 209
Cfr13I GGNCC 1 cut(s) 533
Csp6I GTAC 3 cut(s) 155, 311, 366
CviAII CATG 2 cut(s) 220, 391
CviJI RGCY 7 cut(s) 5, 82, 131, 141, 167, 207, 344
CviKI_1 RGCY 7 cut(s) 5, 82, 131, 141, 167, 207, 344
CviQI GTAC 3 cut(s) 155, 311, 366
DpnI GATC 3 cut(s) 327, 467, 480
DpnII GATC 3 cut(s) 325, 465, 478
DraI TTTAAA 2 cut(s) 162, 406
EaeI YGGCCR 1 cut(s) 3
Eco31I GGTCTC 1 cut(s) 274
Eco47I GGWCC 1 cut(s) 533
Eco57I CTGAAG 1 cut(s) 38
FaeI CATG 2 cut(s) 223, 394
FaiI YATR 8 cut(s) 147, 221, 286, 293, 321, 350, 384, 392
FalI AAGNNNNNCTT 2 cut(s) 84, 116
FaqI GGGAC 2 cut(s) 45, 286
FatI CATG 2 cut(s) 219, 390
FauI CCCGC 2 cut(s) 52, 178
Fnu4HI GCNGC 3 cut(s) 37, 342, 452
FokI GGATG 1 cut(s) 505
Fsp4HI GCNGC 3 cut(s) 37, 342, 452
FspBI CTAG 2 cut(s) 297, 372
GluI GCNGC 3 cut(s) 37, 342, 452
HaeIII GGCC 2 cut(s) 5, 167
HapII CCGG 1 cut(s) 476
Hin1I GRCGYC 1 cut(s) 9
Hin1II CATG 2 cut(s) 223, 394
HindIII AAGCTT 1 cut(s) 80
HpaII CCGG 1 cut(s) 476
HphI GGTGA 1 cut(s) 103
Hpy166II GTNNAC 1 cut(s) 533
Hpy188III TCNNGA 4 cut(s) 89, 194, 372, 476
Hpy8I GTNNAC 1 cut(s) 533
Hpy99I CGWCG 2 cut(s) 11, 14
HpyAV CCTTC 2 cut(s) 197, 324
HpyCH4IV ACGT 1 cut(s) 9
HpyCH4V TGCA 7 cut(s) 36, 145, 219, 355, 378, 415, 451
HpyF10VI GCNNNNNNNGC 1 cut(s) 11
HpySE526I ACGT 1 cut(s) 9
Hsp92I GRCGYC 1 cut(s) 9
Hsp92II CATG 2 cut(s) 223, 394
Kpn2I TCCGGA 1 cut(s) 475
KpnI GGTACC 1 cut(s) 314
Kzo9I GATC 3 cut(s) 325, 465, 478
LmnI GCTCC 1 cut(s) 138
LpnPI CCDG 9 cut(s) 90, 193, 207, 218, 255, 345, 451, 461, 489
Lsp1109I GCAGC 3 cut(s) 48, 353, 463
LweI GCATC 4 cut(s) 85, 365, 483, 500
MaeI CTAG 2 cut(s) 297, 372
MaeII ACGT 1 cut(s) 9
MaeIII GTNAC 1 cut(s) 443
MalI GATC 3 cut(s) 327, 467, 480
MboI GATC 3 cut(s) 325, 465, 478
MboII GAAGA 3 cut(s) 44, 104, 235
MhlI GDGCHC 1 cut(s) 31
MluCI AATT 4 cut(s) 74, 149, 410, 483
MnlI CCTC 2 cut(s) 96, 424
MroI TCCGGA 1 cut(s) 475
MseI TTAA 4 cut(s) 126, 161, 405, 538
MspA1I CMGCKG 1 cut(s) 344
MspI CCGG 1 cut(s) 476
MwoI GCNNNNNNNGC 1 cut(s) 11
NdeII GATC 3 cut(s) 325, 465, 478
NlaIII CATG 2 cut(s) 223, 394
NlaIV GGNNCC 3 cut(s) 312, 421, 435
NmuCI GTSAC 1 cut(s) 443
PflFI GACNNNGTC 1 cut(s) 234
PkrI GCNGC 3 cut(s) 38, 343, 453
PspN4I GGNNCC 3 cut(s) 312, 421, 435
PspPI GGNCC 1 cut(s) 533
PstI CTGCAG 1 cut(s) 453
PsyI GACNNNGTC 1 cut(s) 234
PvuII CAGCTG 1 cut(s) 344
RsaI GTAC 3 cut(s) 156, 312, 367
RsaNI GTAC 3 cut(s) 155, 311, 366
SaqAI TTAA 4 cut(s) 126, 161, 405, 538
SatI GCNGC 3 cut(s) 37, 342, 452
Sau3AI GATC 3 cut(s) 325, 465, 478
Sau96I GGNCC 1 cut(s) 533
SduI GDGCHC 1 cut(s) 31
SfaNI GCATC 4 cut(s) 85, 365, 483, 500
SfcI CTRYAG 1 cut(s) 449
SinI GGWCC 1 cut(s) 533
Sse9I AATT 4 cut(s) 74, 149, 410, 483
SsiI CCGC 2 cut(s) 45, 185
SspI AATATT 1 cut(s) 215
SspMI CTAG 2 cut(s) 297, 372
TaiI ACGT 1 cut(s) 12
TaqI TCGA 2 cut(s) 238, 508
TasI AATT 4 cut(s) 74, 149, 410, 483
Tru1I TTAA 4 cut(s) 126, 161, 405, 538
Tru9I TTAA 4 cut(s) 126, 161, 405, 538
TscAI CASTG 1 cut(s) 62
TseFI GTSAC 1 cut(s) 443
TseI GCWGC 3 cut(s) 36, 341, 451
Tsp45I GTSAC 1 cut(s) 443
TspDTI ATGAA 1 cut(s) 236
TspRI CASTG 1 cut(s) 62
Tth111I GACNNNGTC 1 cut(s) 234
VpaK11BI GGWCC 1 cut(s) 533
XbaI TCTAGA 1 cut(s) 371
XspI CTAG 2 cut(s) 297, 372
ZraI GACGTC 1 cut(s) 10
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.