Rh1CG206800

Removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0003129
Physical Location & Seq
Forward (+)
44831601 .. 44835368
3768 bp
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UTR
Exon/CDS
Intron
Rh1CG206800.1

Sequence Viewer

Length: 432 bp
ATGGCGGGATTTTCTGTGGTGAAATCATATTGTGGTCATGGTATAGGGGAGCTCTTTCATTGTGCACCAAATATTCCTCACTATGCAAGAAATAAAGCAGTTGGAGTGATGAAAGCTGGACAAACTTTTACGATTGAACCAATGATCAATGCAGGAATTTGGCGTGATCGAATGTGGCCTGACGGGTGGACTGATGTTACCGCAGATGGAAAACGAAGTGCTCAATTTGAGCACACACTTCTGGTGACTGAGACCGGAGTTGAAGTTCTTACAGCACGGTTGCCTTCATCGCCAAAAGTGTACCCTTGTATATATAGGTTCGTCTCGGGGCTTCTTCCATGGATACTCAGTTCTCATGATTCTCAACCATGCATTCAGATACAACCCATTTTCTCCATTGACATACATTGCAGATTGATAGACATATATTGA

Protein Analysis

143

Amino Acids

16.01

Weight (kDa)

6.99

Isoelectric Point (pI)

39.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M24 PF00557 3 - 84 2.7e-12 Metallopeptidase family M24
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 5, 201
AcsI RAATTY 1 cut(s) 156
AfaI GTAC 1 cut(s) 302
AgsI TTSAA 2 cut(s) 137, 263
AloI GAACNNNNNNTCC 4 cut(s) 249, 281, 334, 366
AluBI AGCT 2 cut(s) 52, 116
AluI AGCT 2 cut(s) 52, 116
Alw21I GWGCWC 4 cut(s) 54, 67, 223, 234
Alw26I GTCTC 2 cut(s) 245, 328
Alw44I GTGCAC 1 cut(s) 63
Ama87I CYCGRG 1 cut(s) 325
AoxI GGCC 1 cut(s) 176
ApaLI GTGCAC 1 cut(s) 63
ApoI RAATTY 1 cut(s) 156
AsuHPI GGTGA 2 cut(s) 31, 256
AvaI CYCGRG 1 cut(s) 325
BaeGI GKGCMC 1 cut(s) 67
BanII GRGCYC 1 cut(s) 54
Bbv12I GWGCWC 4 cut(s) 54, 67, 223, 234
BccI CCATC 1 cut(s) 200
BciVI GTATCC 1 cut(s) 336
BclI TGATCA 1 cut(s) 144
BcoDI GTCTC 2 cut(s) 245, 328
BfuI GTATCC 1 cut(s) 336
BmeT110I CYCGRG 1 cut(s) 325
BsaI GGTCTC 1 cut(s) 245
BsaJI CCNNGG 1 cut(s) 338
BsaWI WCCGGW 1 cut(s) 254
BsaXI ACNNNNNCTCC 2 cut(s) 249, 279
Bse3DI GCAATG 1 cut(s) 406
BseDI CCNNGG 1 cut(s) 338
BseMI GCAATG 1 cut(s) 406
BseMII CTCAG 2 cut(s) 240, 361
BseSI GKGCMC 1 cut(s) 67
BshFI GGCC 1 cut(s) 178
BsiHKAI GWGCWC 4 cut(s) 54, 67, 223, 234
BsiHKCI CYCGRG 1 cut(s) 325
BsiSI CCGG 1 cut(s) 255
BsmAI GTCTC 2 cut(s) 245, 328
BsmBI CGTCTC 1 cut(s) 328
BsmI GAATGC 1 cut(s) 372
BsnI GGCC 1 cut(s) 178
Bso31I GGTCTC 1 cut(s) 245
BsoBI CYCGRG 1 cut(s) 325
Bsp1286I GDGCHC 4 cut(s) 54, 67, 223, 234
Bsp143I GATC 2 cut(s) 144, 166
Bsp19I CCATGG 1 cut(s) 338
BspACI CCGC 2 cut(s) 5, 201
BspANI GGCC 1 cut(s) 178
BspCNI CTCAG 2 cut(s) 241, 360
BspHI TCATGA 1 cut(s) 355
BspTNI GGTCTC 1 cut(s) 245
BsrDI GCAATG 1 cut(s) 406
BssECI CCNNGG 1 cut(s) 338
BssMI GATC 2 cut(s) 144, 166
BssT1I CCWWGG 1 cut(s) 338
Bst4CI ACNGT 1 cut(s) 279
BstDEI CTNAG 2 cut(s) 249, 347
BstDSI CCRYGG 1 cut(s) 338
BstKTI GATC 2 cut(s) 147, 169
BstMAI GTCTC 2 cut(s) 245, 328
BstMBI GATC 2 cut(s) 144, 166
BstMWI GCNNNNNNNGC 1 cut(s) 289
BstSLI GKGCMC 1 cut(s) 67
BsuI GTATCC 1 cut(s) 336
BsuRI GGCC 1 cut(s) 178
BtgI CCRYGG 1 cut(s) 338
BtgZI GCGATG 1 cut(s) 273
CciI TCATGA 1 cut(s) 355
Csp6I GTAC 1 cut(s) 301
CviAII CATG 4 cut(s) 38, 339, 356, 369
CviJI RGCY 4 cut(s) 52, 116, 178, 331
CviKI_1 RGCY 4 cut(s) 52, 116, 178, 331
CviQI GTAC 1 cut(s) 301
DdeI CTNAG 2 cut(s) 249, 347
DpnI GATC 2 cut(s) 146, 168
DpnII GATC 2 cut(s) 144, 166
Ecl136II GAGCTC 1 cut(s) 52
Eco130I CCWWGG 1 cut(s) 338
Eco24I GRGCYC 1 cut(s) 54
Eco31I GGTCTC 1 cut(s) 245
Eco53kI GAGCTC 1 cut(s) 52
Eco88I CYCGRG 1 cut(s) 325
EcoICRI GAGCTC 1 cut(s) 52
EcoT14I CCWWGG 1 cut(s) 338
EcoT22I ATGCAT 1 cut(s) 374
EcoT38I GRGCYC 1 cut(s) 54
ErhI CCWWGG 1 cut(s) 338
Esp3I CGTCTC 1 cut(s) 328
FaeI CATG 4 cut(s) 41, 342, 359, 372
FatI CATG 4 cut(s) 37, 338, 355, 368
FbaI TGATCA 1 cut(s) 144
FriOI GRGCYC 1 cut(s) 54
HaeIII GGCC 1 cut(s) 178
HapII CCGG 1 cut(s) 255
Hin1II CATG 4 cut(s) 41, 342, 359, 372
HinfI GANTC 1 cut(s) 359
HpaII CCGG 1 cut(s) 255
HphI GGTGA 2 cut(s) 31, 256
Hpy166II GTNNAC 3 cut(s) 65, 189, 301
Hpy188I TCNGA 1 cut(s) 378
Hpy188III TCNNGA 1 cut(s) 356
Hpy8I GTNNAC 3 cut(s) 65, 189, 301
HpyAV CCTTC 1 cut(s) 294
HpyCH4III ACNGT 1 cut(s) 279
HpyCH4V TGCA 5 cut(s) 65, 86, 152, 372, 411
HpyF10VI GCNNNNNNNGC 1 cut(s) 289
HpyF3I CTNAG 2 cut(s) 249, 347
Hsp92II CATG 4 cut(s) 41, 342, 359, 372
Ksp22I TGATCA 1 cut(s) 144
Kzo9I GATC 2 cut(s) 144, 166
LmnI GCTCC 1 cut(s) 49
LpnPI CCDG 5 cut(s) 102, 138, 192, 227, 268
MaeIII GTNAC 2 cut(s) 196, 244
MalI GATC 2 cut(s) 146, 168
MboI GATC 2 cut(s) 144, 166
MboII GAAGA 1 cut(s) 326
MhlI GDGCHC 4 cut(s) 54, 67, 223, 234
MluCI AATT 2 cut(s) 156, 224
MmeI TCCRAC 1 cut(s) 82
MnlI CCTC 1 cut(s) 87
Mph1103I ATGCAT 1 cut(s) 374
MspI CCGG 1 cut(s) 255
Mva1269I GAATGC 1 cut(s) 372
MwoI GCNNNNNNNGC 1 cut(s) 289
NcoI CCATGG 1 cut(s) 338
NdeII GATC 2 cut(s) 144, 166
NlaIII CATG 4 cut(s) 41, 342, 359, 372
NmuCI GTSAC 1 cut(s) 244
NsiI ATGCAT 1 cut(s) 374
PagI TCATGA 1 cut(s) 355
PctI GAATGC 1 cut(s) 372
PfeI GAWTC 1 cut(s) 359
Psp124BI GAGCTC 1 cut(s) 54
RsaI GTAC 1 cut(s) 302
RsaNI GTAC 1 cut(s) 301
SacI GAGCTC 1 cut(s) 54
Sau3AI GATC 2 cut(s) 144, 166
SduI GDGCHC 4 cut(s) 54, 67, 223, 234
SetI ASST 3 cut(s) 54, 118, 320
Sse9I AATT 2 cut(s) 156, 224
SsiI CCGC 2 cut(s) 5, 201
SspI AATATT 1 cut(s) 73
SstI GAGCTC 1 cut(s) 54
StyI CCWWGG 1 cut(s) 338
TaaI ACNGT 1 cut(s) 279
TaqI TCGA 1 cut(s) 169
TasI AATT 2 cut(s) 156, 224
TfiI GAWTC 1 cut(s) 359
TseFI GTSAC 1 cut(s) 244
Tsp45I GTSAC 1 cut(s) 244
TspDTI ATGAA 3 cut(s) 47, 125, 276
VneI GTGCAC 1 cut(s) 63
XapI RAATTY 1 cut(s) 156
Zsp2I ATGCAT 1 cut(s) 374
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.