Probably involved in the RNA silencing pathway and required for the generation of small interfering RNAs (siRNAs)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 3 cut(s) | 327, 657, 1761 |
| Acc65I | GGTACC | 1 cut(s) | 2940 |
| AccB1I | GGYRCC | 3 cut(s) | 4, 311, 2940 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 2086, 2403 |
| AccI | GTMKAC | 1 cut(s) | 864 |
| AccII | CGCG | 1 cut(s) | 2203 |
| AccIII | TCCGGA | 1 cut(s) | 1300 |
| AclWI | GGATC | 9 cut(s) | 172, 662, 675, 1460, 1626, 1673, 2578, 2621, 2876 |
| AcsI | RAATTY | 4 cut(s) | 284, 601, 1126, 1370 |
| AcuI | CTGAAG | 4 cut(s) | 524, 939, 1772, 2760 |
| AcvI | CACGTG | 1 cut(s) | 221 |
| AdeI | CACNNNGTG | 1 cut(s) | 2086 |
| AfaI | GTAC | 5 cut(s) | 770, 1006, 1559, 2439, 2942 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 563, 2086, 2111, 2403, 2432, 2601 |
| AflIII | ACRYGT | 3 cut(s) | 257, 765, 1841 |
| AgeI | ACCGGT | 1 cut(s) | 2432 |
| AhdI | GACNNNNNGTC | 2 cut(s) | 2465, 2806 |
| AhlI | ACTAGT | 1 cut(s) | 2440 |
| AjnI | CCWGG | 5 cut(s) | 705, 1229, 1256, 1542, 2032 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 1322, 1354, 2297, 2329 |
| Alw21I | GWGCWC | 2 cut(s) | 138, 445 |
| Alw26I | GTCTC | 3 cut(s) | 1173, 1256, 2991 |
| Alw44I | GTGCAC | 2 cut(s) | 134, 441 |
| AlwI | GGATC | 9 cut(s) | 172, 662, 675, 1460, 1626, 1673, 2578, 2621, 2876 |
| Ama87I | CYCGRG | 1 cut(s) | 629 |
| Aor13HI | TCCGGA | 1 cut(s) | 1300 |
| AoxI | GGCC | 3 cut(s) | 820, 1156, 2120 |
| ApaLI | GTGCAC | 2 cut(s) | 134, 441 |
| ApeKI | GCWGC | 5 cut(s) | 208, 1226, 2145, 2310, 2921 |
| ApoI | RAATTY | 4 cut(s) | 284, 601, 1126, 1370 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1472, 1504 |
| AseI | ATTAAT | 1 cut(s) | 2664 |
| AsiGI | ACCGGT | 1 cut(s) | 2432 |
| Asp700I | GAANNNNTTC | 3 cut(s) | 1296, 1668, 2540 |
| Asp718I | GGTACC | 1 cut(s) | 2940 |
| AspS9I | GGNCC | 3 cut(s) | 875, 1156, 2406 |
| AsuC2I | CCSGG | 3 cut(s) | 630, 631, 1894 |
| AsuHPI | GGTGA | 6 cut(s) | 789, 1088, 1202, 1925, 2198, 2758 |
| AsuII | TTCGAA | 2 cut(s) | 1515, 3030 |
| AvaI | CYCGRG | 1 cut(s) | 629 |
| AvaII | GGWCC | 2 cut(s) | 875, 2406 |
| BaeGI | GKGCMC | 3 cut(s) | 138, 316, 445 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 2757, 2790 |
| BamHI | GGATCC | 1 cut(s) | 667 |
| BanI | GGYRCC | 3 cut(s) | 4, 311, 2940 |
| BarI | GAAGNNNNNNTAC | 4 cut(s) | 1144, 1176, 2733, 2765 |
| BbrPI | CACGTG | 1 cut(s) | 221 |
| Bbv12I | GWGCWC | 2 cut(s) | 138, 445 |
| BbvCI | CCTCAGC | 1 cut(s) | 1011 |
| BbvI | GCAGC | 5 cut(s) | 195, 1238, 2157, 2322, 2933 |
| BceAI | ACGGC | 1 cut(s) | 1762 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 1089, 1099, 1123, 1133 |
| BciT130I | CCWGG | 5 cut(s) | 707, 1231, 1258, 1544, 2034 |
| BciVI | GTATCC | 1 cut(s) | 1035 |
| BcnI | CCSGG | 3 cut(s) | 630, 631, 1894 |
| BcoDI | GTCTC | 3 cut(s) | 1173, 1256, 2991 |
| BcuI | ACTAGT | 1 cut(s) | 2440 |
| BfaI | CTAG | 7 cut(s) | 662, 675, 1097, 1883, 2231, 2441, 2601 |
| BfmI | CTRYAG | 1 cut(s) | 981 |
| BfuI | GTATCC | 1 cut(s) | 1035 |
| BglII | AGATCT | 1 cut(s) | 1865 |
| BisI | GCNGC | 7 cut(s) | 209, 1227, 2146, 2204, 2311, 2453, 2922 |
| BlsI | GCNGC | 7 cut(s) | 210, 1228, 2147, 2205, 2312, 2454, 2923 |
| BmcAI | AGTACT | 1 cut(s) | 1006 |
| Bme1390I | CCNGG | 8 cut(s) | 630, 631, 707, 1231, 1258, 1544, 1894, 2034 |
| Bme18I | GGWCC | 2 cut(s) | 875, 2406 |
| BmeRI | GACNNNNNGTC | 2 cut(s) | 2465, 2806 |
| BmeT110I | CYCGRG | 1 cut(s) | 629 |
| BmgT120I | GGNCC | 3 cut(s) | 875, 1156, 2406 |
| BmiI | GGNNCC | 9 cut(s) | 6, 313, 348, 555, 669, 1157, 1327, 2408, 2942 |
| BmrFI | CCNGG | 8 cut(s) | 630, 631, 707, 1231, 1258, 1544, 1894, 2034 |
| BmrI | ACTGGG | 1 cut(s) | 2977 |
| BmsI | GCATC | 8 cut(s) | 41, 405, 963, 1782, 1820, 2328, 2659, 2939 |
| BmuI | ACTGGG | 1 cut(s) | 2977 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 167, 199 |
| BpmI | CTGGAG | 1 cut(s) | 2055 |
| Bpu10I | CCTNAGC | 2 cut(s) | 1011, 2140 |
| Bpu14I | TTCGAA | 2 cut(s) | 1515, 3030 |
| BpuEI | CTTGAG | 1 cut(s) | 194 |
| BpuMI | CCSGG | 3 cut(s) | 630, 631, 1894 |
| BsaAI | YACGTR | 4 cut(s) | 221, 258, 308, 1844 |
| BsaI | GGTCTC | 1 cut(s) | 2991 |
| BsaWI | WCCGGW | 2 cut(s) | 1300, 2432 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 331, 361, 1012, 1042 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 563, 2086, 2111, 2403, 2432, 2601 |
| Bse118I | RCCGGY | 2 cut(s) | 2068, 2432 |
| Bse1I | ACTGG | 8 cut(s) | 301, 449, 1026, 1187, 1954, 2253, 2761, 2972 |
| Bse3DI | GCAATG | 2 cut(s) | 1656, 1931 |
| BseAI | TCCGGA | 1 cut(s) | 1300 |
| BseBI | CCWGG | 5 cut(s) | 707, 1231, 1258, 1544, 2034 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 563, 2086, 2111, 2403, 2432, 2601 |
| BseMI | GCAATG | 2 cut(s) | 1656, 1931 |
| BseMII | CTCAG | 6 cut(s) | 54, 806, 1002, 1293, 2154, 2999 |
| BseNI | ACTGG | 8 cut(s) | 301, 449, 1026, 1187, 1954, 2253, 2761, 2972 |
| BseRI | GAGGAG | 3 cut(s) | 80, 1646, 2294 |
| BseSI | GKGCMC | 3 cut(s) | 138, 316, 445 |
| BseXI | GCAGC | 5 cut(s) | 195, 1238, 2157, 2322, 2933 |
| BseYI | CCCAGC | 3 cut(s) | 360, 2178, 2704 |
| BsgI | GTGCAG | 1 cut(s) | 1149 |
| Bsh1236I | CGCG | 1 cut(s) | 2203 |
| BshFI | GGCC | 3 cut(s) | 822, 1158, 2122 |
| BshNI | GGYRCC | 3 cut(s) | 4, 311, 2940 |
| BshTI | ACCGGT | 1 cut(s) | 2432 |
| BsiHKAI | GWGCWC | 2 cut(s) | 138, 445 |
| BsiHKCI | CYCGRG | 1 cut(s) | 629 |
| BsiSI | CCGG | 7 cut(s) | 199, 525, 630, 1301, 1893, 2069, 2433 |
| BslFI | GGGAC | 3 cut(s) | 248, 1909, 2392 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 563, 2086, 2111, 2403, 2432, 2601 |
| BsmAI | GTCTC | 3 cut(s) | 1173, 1256, 2991 |
| BsmFI | GGGAC | 3 cut(s) | 248, 1909, 2392 |
| BsmI | GAATGC | 1 cut(s) | 2141 |
| BsnI | GGCC | 3 cut(s) | 822, 1158, 2122 |
| Bso31I | GGTCTC | 1 cut(s) | 2991 |
| BsoBI | CYCGRG | 1 cut(s) | 629 |
| Bsp119I | TTCGAA | 2 cut(s) | 1515, 3030 |
| Bsp1286I | GDGCHC | 3 cut(s) | 138, 316, 445 |
| Bsp13I | TCCGGA | 1 cut(s) | 1300 |
| Bsp1407I | TGTACA | 1 cut(s) | 768 |
| Bsp19I | CCATGG | 1 cut(s) | 3017 |
| BspANI | GGCC | 3 cut(s) | 822, 1158, 2122 |
| BspCNI | CTCAG | 6 cut(s) | 55, 805, 1003, 1292, 2153, 3000 |
| BspEI | TCCGGA | 1 cut(s) | 1300 |
| BspFNI | CGCG | 1 cut(s) | 2203 |
| BspLI | GGNNCC | 9 cut(s) | 6, 313, 348, 555, 669, 1157, 1327, 2408, 2942 |
| BspPI | GGATC | 9 cut(s) | 172, 662, 675, 1460, 1626, 1673, 2578, 2621, 2876 |
| BspQI | GCTCTTC | 1 cut(s) | 1258 |
| BspT104I | TTCGAA | 2 cut(s) | 1515, 3030 |
| BspT107I | GGYRCC | 3 cut(s) | 4, 311, 2940 |
| BspTNI | GGTCTC | 1 cut(s) | 2991 |
| BsrDI | GCAATG | 2 cut(s) | 1656, 1931 |
| BsrFI | RCCGGY | 2 cut(s) | 2068, 2432 |
| BsrGI | TGTACA | 1 cut(s) | 768 |
| BsrI | ACTGG | 8 cut(s) | 301, 449, 1026, 1187, 1954, 2253, 2761, 2972 |
| BssAI | RCCGGY | 2 cut(s) | 2068, 2432 |
| BssNAI | GTATAC | 1 cut(s) | 865 |
| BssT1I | CCWWGG | 4 cut(s) | 726, 1606, 2566, 3017 |
| Bst1107I | GTATAC | 1 cut(s) | 865 |
| Bst2UI | CCWGG | 5 cut(s) | 707, 1231, 1258, 1544, 2034 |
| Bst4CI | ACNGT | 9 cut(s) | 495, 620, 699, 717, 1273, 1439, 1990, 2242, 2856 |
| Bst6I | CTCTTC | 3 cut(s) | 415, 500, 1258 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 2461 |
| BstAUI | TGTACA | 1 cut(s) | 768 |
| BstBAI | YACGTR | 4 cut(s) | 221, 258, 308, 1844 |
| BstBI | TTCGAA | 2 cut(s) | 1515, 3030 |
| BstC8I | GCNNGC | 5 cut(s) | 636, 778, 782, 1972, 2070 |
| BstDEI | CTNAG | 8 cut(s) | 63, 792, 1011, 1055, 1279, 2140, 2613, 3008 |
| BstDSI | CCRYGG | 2 cut(s) | 2201, 3017 |
| BstEII | GGTNACC | 1 cut(s) | 2186 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 2599 |
| BstFNI | CGCG | 1 cut(s) | 2203 |
| BstMAI | GTCTC | 3 cut(s) | 1173, 1256, 2991 |
| BstMWI | GCNNNNNNNGC | 8 cut(s) | 1223, 1373, 1440, 1697, 2145, 2316, 2461, 2927 |
| BstNI | CCWGG | 5 cut(s) | 707, 1231, 1258, 1544, 2034 |
| BstNSI | RCATGY | 4 cut(s) | 769, 780, 1507, 1738 |
| BstPI | GGTNACC | 1 cut(s) | 2186 |
| BstSCI | CCNGG | 8 cut(s) | 628, 629, 705, 1229, 1256, 1542, 1892, 2032 |
| BstSFI | CTRYAG | 1 cut(s) | 981 |
| BstSLI | GKGCMC | 3 cut(s) | 138, 316, 445 |
| BstUI | CGCG | 1 cut(s) | 2203 |
| BstV1I | GCAGC | 5 cut(s) | 195, 1238, 2157, 2322, 2933 |
| BstX2I | RGATCY | 3 cut(s) | 177, 667, 1865 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 308 |
| BstYI | RGATCY | 3 cut(s) | 177, 667, 1865 |
| BstZ17I | GTATAC | 1 cut(s) | 865 |
| BsuI | GTATCC | 1 cut(s) | 1035 |
| BsuRI | GGCC | 3 cut(s) | 822, 1158, 2122 |
| BtgI | CCRYGG | 2 cut(s) | 2201, 3017 |
| BtsIMutI | CAGTG | 3 cut(s) | 1033, 1700, 1947 |
| Cac8I | GCNNGC | 5 cut(s) | 636, 778, 782, 1972, 2070 |
| Cfr10I | RCCGGY | 2 cut(s) | 2068, 2432 |
| Cfr13I | GGNCC | 3 cut(s) | 875, 1156, 2406 |
| Cfr42I | CCGCGG | 1 cut(s) | 2204 |
| Cfr9I | CCCGGG | 1 cut(s) | 629 |
| Csp6I | GTAC | 5 cut(s) | 769, 1005, 1558, 2438, 2941 |
| CspAI | ACCGGT | 1 cut(s) | 2432 |
| CviQI | GTAC | 5 cut(s) | 769, 1005, 1558, 2438, 2941 |
| DdeI | CTNAG | 8 cut(s) | 63, 792, 1011, 1055, 1279, 2140, 2613, 3008 |
| DraI | TTTAAA | 2 cut(s) | 232, 1717 |
| DraIII | CACNNNGTG | 1 cut(s) | 2086 |
| DriI | GACNNNNNGTC | 2 cut(s) | 2465, 2806 |
| Eam1104I | CTCTTC | 3 cut(s) | 415, 500, 1258 |
| Eam1105I | GACNNNNNGTC | 2 cut(s) | 2465, 2806 |
| EarI | CTCTTC | 3 cut(s) | 415, 500, 1258 |
| EciI | GGCGGA | 2 cut(s) | 41, 1338 |
| Eco130I | CCWWGG | 4 cut(s) | 726, 1606, 2566, 3017 |
| Eco147I | AGGCCT | 2 cut(s) | 822, 2122 |
| Eco31I | GGTCTC | 1 cut(s) | 2991 |
| Eco32I | GATATC | 4 cut(s) | 60, 925, 1362, 2905 |
| Eco47I | GGWCC | 2 cut(s) | 875, 2406 |
| Eco57I | CTGAAG | 4 cut(s) | 524, 939, 1772, 2760 |
| Eco72I | CACGTG | 1 cut(s) | 221 |
| Eco88I | CYCGRG | 1 cut(s) | 629 |
| Eco91I | GGTNACC | 1 cut(s) | 2186 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 2599 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1156 |
| EcoO65I | GGTNACC | 1 cut(s) | 2186 |
| EcoRI | GAATTC | 1 cut(s) | 1126 |
| EcoRII | CCWGG | 5 cut(s) | 705, 1229, 1256, 1542, 2032 |
| EcoRV | GATATC | 4 cut(s) | 60, 925, 1362, 2905 |
| EcoT14I | CCWWGG | 4 cut(s) | 726, 1606, 2566, 3017 |
| ErhI | CCWWGG | 4 cut(s) | 726, 1606, 2566, 3017 |
| FaqI | GGGAC | 3 cut(s) | 248, 1909, 2392 |
| FblI | GTMKAC | 1 cut(s) | 864 |
| Fnu4HI | GCNGC | 7 cut(s) | 209, 1227, 2146, 2204, 2311, 2453, 2922 |
| Fsp4HI | GCNGC | 7 cut(s) | 209, 1227, 2146, 2204, 2311, 2453, 2922 |
| FspBI | CTAG | 7 cut(s) | 662, 675, 1097, 1883, 2231, 2441, 2601 |
| GluI | GCNGC | 7 cut(s) | 209, 1227, 2146, 2204, 2311, 2453, 2922 |
| GsaI | CCCAGC | 3 cut(s) | 364, 2182, 2708 |
| GsuI | CTGGAG | 1 cut(s) | 2055 |
| HaeIII | GGCC | 3 cut(s) | 822, 1158, 2122 |
| HapII | CCGG | 7 cut(s) | 199, 525, 630, 1301, 1893, 2069, 2433 |
| HincII | GTYRAC | 1 cut(s) | 1583 |
| HindII | GTYRAC | 1 cut(s) | 1583 |
| HindIII | AAGCTT | 2 cut(s) | 170, 2537 |
| HpaII | CCGG | 7 cut(s) | 199, 525, 630, 1301, 1893, 2069, 2433 |
| HphI | GGTGA | 6 cut(s) | 789, 1088, 1202, 1925, 2198, 2758 |
| Hpy166II | GTNNAC | 9 cut(s) | 136, 218, 443, 472, 865, 1583, 1602, 2027, 2438 |
| Hpy8I | GTNNAC | 9 cut(s) | 136, 218, 443, 472, 865, 1583, 1602, 2027, 2438 |
| Hpy99I | CGWCG | 1 cut(s) | 1111 |
| HpyAV | CCTTC | 8 cut(s) | 276, 464, 812, 1146, 1658, 1690, 2359, 2605 |
| HpyCH4III | ACNGT | 9 cut(s) | 495, 620, 699, 717, 1273, 1439, 1990, 2242, 2856 |
| HpyCH4IV | ACGT | 4 cut(s) | 220, 257, 307, 1843 |
| HpyF10VI | GCNNNNNNNGC | 8 cut(s) | 1223, 1373, 1440, 1697, 2145, 2316, 2461, 2927 |
| HpyF3I | CTNAG | 8 cut(s) | 63, 792, 1011, 1055, 1279, 2140, 2613, 3008 |
| HpySE526I | ACGT | 4 cut(s) | 220, 257, 307, 1843 |
| Kpn2I | TCCGGA | 1 cut(s) | 1300 |
| KpnI | GGTACC | 1 cut(s) | 2944 |
| KroI | GCCGGC | 1 cut(s) | 2068 |
| KroNI | GCCGGC | 1 cut(s) | 2070 |
| KspI | CCGCGG | 1 cut(s) | 2204 |
| LguI | GCTCTTC | 1 cut(s) | 1258 |
| LmnI | GCTCC | 4 cut(s) | 352, 424, 559, 1551 |
| Lsp1109I | GCAGC | 5 cut(s) | 195, 1238, 2157, 2322, 2933 |
| LweI | GCATC | 8 cut(s) | 41, 405, 963, 1782, 1820, 2328, 2659, 2939 |
| MaeI | CTAG | 7 cut(s) | 662, 675, 1097, 1883, 2231, 2441, 2601 |
| MaeII | ACGT | 4 cut(s) | 220, 257, 307, 1843 |
| MflI | RGATCY | 3 cut(s) | 177, 667, 1865 |
| MhlI | GDGCHC | 3 cut(s) | 138, 316, 445 |
| MlyI | GAGTC | 5 cut(s) | 1120, 2291, 2525, 2816, 2875 |
| MmeI | TCCRAC | 2 cut(s) | 1493, 2569 |
| MroI | TCCGGA | 1 cut(s) | 1300 |
| MroNI | GCCGGC | 1 cut(s) | 2068 |
| MroXI | GAANNNNTTC | 3 cut(s) | 1296, 1668, 2540 |
| MslI | CAYNNNNRTG | 3 cut(s) | 436, 2130, 2935 |
| MspA1I | CMGCKG | 3 cut(s) | 360, 1660, 2203 |
| MspI | CCGG | 7 cut(s) | 199, 525, 630, 1301, 1893, 2069, 2433 |
| MspR9I | CCNGG | 8 cut(s) | 630, 631, 707, 1231, 1258, 1544, 1894, 2034 |
| Mva1269I | GAATGC | 1 cut(s) | 2141 |
| MvaI | CCWGG | 5 cut(s) | 707, 1231, 1258, 1544, 2034 |
| MvnI | CGCG | 1 cut(s) | 2203 |
| MwoI | GCNNNNNNNGC | 8 cut(s) | 1223, 1373, 1440, 1697, 2145, 2316, 2461, 2927 |
| NaeI | GCCGGC | 1 cut(s) | 2070 |
| NciI | CCSGG | 3 cut(s) | 630, 631, 1894 |
| NcoI | CCATGG | 1 cut(s) | 3017 |
| NgoMIV | GCCGGC | 1 cut(s) | 2068 |
| NlaIV | GGNNCC | 9 cut(s) | 6, 313, 348, 555, 669, 1157, 1327, 2408, 2942 |
| NmeAIII | GCCGAG | 1 cut(s) | 2000 |
| NmuCI | GTSAC | 7 cut(s) | 34, 106, 512, 795, 2186, 2498, 2678 |
| NspI | RCATGY | 4 cut(s) | 769, 780, 1507, 1738 |
| NspV | TTCGAA | 2 cut(s) | 1515, 3030 |
| PaeI | GCATGC | 1 cut(s) | 780 |
| PceI | AGGCCT | 2 cut(s) | 822, 2122 |
| PciI | ACATGT | 1 cut(s) | 765 |
| PciSI | GCTCTTC | 1 cut(s) | 1258 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 904 |
| PctI | GAATGC | 1 cut(s) | 2141 |
| PdiI | GCCGGC | 1 cut(s) | 2070 |
| PdmI | GAANNNNTTC | 3 cut(s) | 1296, 1668, 2540 |
| PfeI | GAWTC | 8 cut(s) | 585, 787, 1517, 1654, 1669, 2194, 2420, 2649 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 2086, 2403 |
| PinAI | ACCGGT | 1 cut(s) | 2432 |
| PkrI | GCNGC | 7 cut(s) | 210, 1228, 2147, 2205, 2312, 2454, 2923 |
| PleI | GAGTC | 5 cut(s) | 1119, 2290, 2524, 2815, 2874 |
| PmaCI | CACGTG | 1 cut(s) | 221 |
| PmlI | CACGTG | 1 cut(s) | 221 |
| PpsI | GAGTC | 5 cut(s) | 1119, 2290, 2524, 2815, 2874 |
| Ppu21I | YACGTR | 4 cut(s) | 221, 258, 308, 1844 |
| PscI | ACATGT | 1 cut(s) | 765 |
| PshBI | ATTAAT | 1 cut(s) | 2664 |
| PsiI | TTATAA | 3 cut(s) | 327, 657, 1761 |
| Psp6I | CCWGG | 5 cut(s) | 705, 1229, 1256, 1542, 2032 |
| PspCI | CACGTG | 1 cut(s) | 221 |
| PspEI | GGTNACC | 1 cut(s) | 2186 |
| PspFI | CCCAGC | 3 cut(s) | 360, 2178, 2704 |
| PspGI | CCWGG | 5 cut(s) | 705, 1229, 1256, 1542, 2032 |
| PspN4I | GGNNCC | 9 cut(s) | 6, 313, 348, 555, 669, 1157, 1327, 2408, 2942 |
| PspPI | GGNCC | 3 cut(s) | 875, 1156, 2406 |
| PsuI | RGATCY | 3 cut(s) | 177, 667, 1865 |
| PvuII | CAGCTG | 1 cut(s) | 1660 |
| RsaI | GTAC | 5 cut(s) | 770, 1006, 1559, 2439, 2942 |
| RsaNI | GTAC | 5 cut(s) | 769, 1005, 1558, 2438, 2941 |
| RseI | CAYNNNNRTG | 3 cut(s) | 436, 2130, 2935 |
| SacII | CCGCGG | 1 cut(s) | 2204 |
| SapI | GCTCTTC | 1 cut(s) | 1258 |
| SatI | GCNGC | 7 cut(s) | 209, 1227, 2146, 2204, 2311, 2453, 2922 |
| Sau96I | GGNCC | 3 cut(s) | 875, 1156, 2406 |
| ScaI | AGTACT | 1 cut(s) | 1006 |
| SchI | GAGTC | 5 cut(s) | 1120, 2291, 2525, 2816, 2875 |
| ScrFI | CCNGG | 8 cut(s) | 630, 631, 707, 1231, 1258, 1544, 1894, 2034 |
| SduI | GDGCHC | 3 cut(s) | 138, 316, 445 |
| SfaNI | GCATC | 8 cut(s) | 41, 405, 963, 1782, 1820, 2328, 2659, 2939 |
| SfcI | CTRYAG | 1 cut(s) | 981 |
| Sfr303I | CCGCGG | 1 cut(s) | 2204 |
| SfuI | TTCGAA | 2 cut(s) | 1515, 3030 |
| SgrBI | CCGCGG | 1 cut(s) | 2204 |
| SinI | GGWCC | 2 cut(s) | 875, 2406 |
| SmaI | CCCGGG | 1 cut(s) | 631 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 436, 2130, 2935 |
| SmlI | CTYRAG | 1 cut(s) | 173 |
| SmoI | CTYRAG | 1 cut(s) | 173 |
| SpeI | ACTAGT | 1 cut(s) | 2440 |
| SphI | GCATGC | 1 cut(s) | 780 |
| SseBI | AGGCCT | 2 cut(s) | 822, 2122 |
| SspI | AATATT | 1 cut(s) | 1334 |
| SspMI | CTAG | 7 cut(s) | 662, 675, 1097, 1883, 2231, 2441, 2601 |
| StuI | AGGCCT | 2 cut(s) | 822, 2122 |
| StyD4I | CCNGG | 8 cut(s) | 628, 629, 705, 1229, 1256, 1542, 1892, 2032 |
| StyI | CCWWGG | 4 cut(s) | 726, 1606, 2566, 3017 |
| TaaI | ACNGT | 9 cut(s) | 495, 620, 699, 717, 1273, 1439, 1990, 2242, 2856 |
| TaiI | ACGT | 4 cut(s) | 223, 260, 310, 1846 |
| TaqI | TCGA | 8 cut(s) | 367, 927, 1109, 1464, 1515, 2810, 2862, 3030 |
| TatI | WGTACW | 3 cut(s) | 768, 1004, 2437 |
| TauI | GCSGC | 2 cut(s) | 2206, 2455 |
| TfiI | GAWTC | 8 cut(s) | 585, 787, 1517, 1654, 1669, 2194, 2420, 2649 |
| TscAI | CASTG | 3 cut(s) | 1033, 1707, 1954 |
| TseFI | GTSAC | 7 cut(s) | 34, 106, 512, 795, 2186, 2498, 2678 |
| TseI | GCWGC | 5 cut(s) | 208, 1226, 2145, 2310, 2921 |
| Tsp45I | GTSAC | 7 cut(s) | 34, 106, 512, 795, 2186, 2498, 2678 |
| TspGWI | ACGGA | 1 cut(s) | 1362 |
| TspMI | CCCGGG | 1 cut(s) | 629 |
| TspRI | CASTG | 3 cut(s) | 1033, 1707, 1954 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 2086, 2403 |
| VneI | GTGCAC | 2 cut(s) | 134, 441 |
| VpaK11BI | GGWCC | 2 cut(s) | 875, 2406 |
| VspI | ATTAAT | 1 cut(s) | 2664 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 2599 |
| XapI | RAATTY | 4 cut(s) | 284, 601, 1126, 1370 |
| XbaI | TCTAGA | 1 cut(s) | 1882 |
| XceI | RCATGY | 4 cut(s) | 769, 780, 1507, 1738 |
| XmaI | CCCGGG | 1 cut(s) | 629 |
| XmiI | GTMKAC | 1 cut(s) | 864 |
| XmnI | GAANNNNTTC | 3 cut(s) | 1296, 1668, 2540 |
| XspI | CTAG | 7 cut(s) | 662, 675, 1097, 1883, 2231, 2441, 2601 |
| ZrmI | AGTACT | 1 cut(s) | 1006 |