Probably involved in the RNA silencing pathway and required for the generation of small interfering RNAs (siRNAs)
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 4 cut(s) | 534, 864, 1980, 3021 |
| Acc65I | GGTACC | 1 cut(s) | 4200 |
| AccB1I | GGYRCC | 3 cut(s) | 262, 518, 4200 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 125, 3346 |
| AccI | GTMKAC | 4 cut(s) | 77, 1050, 1080, 3678 |
| AccII | CGCG | 2 cut(s) | 1213, 2254 |
| AccIII | TCCGGA | 2 cut(s) | 731, 2560 |
| AcsI | RAATTY | 9 cut(s) | 62, 736, 808, 1345, 1589, 2064, 2386, 2630, 3105 |
| AcuI | CTGAAG | 5 cut(s) | 731, 1155, 2196, 4020, 4371 |
| AcvI | CACGTG | 1 cut(s) | 479 |
| AdeI | CACNNNGTG | 1 cut(s) | 3346 |
| AfaI | GTAC | 6 cut(s) | 977, 986, 1527, 1778, 2819, 4202 |
| AflII | CTTAAG | 1 cut(s) | 968 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 4066 |
| AjiI | CACGTC | 1 cut(s) | 3325 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 1541, 1573, 2582, 2614 |
| AloI | GAACNNNNNNTCC | 6 cut(s) | 908, 940, 1916, 1948, 2957, 2989 |
| Alw21I | GWGCWC | 3 cut(s) | 396, 438, 652 |
| Alw26I | GTCTC | 7 cut(s) | 48, 1392, 1475, 2433, 2516, 3441, 4251 |
| Alw44I | GTGCAC | 2 cut(s) | 392, 648 |
| Ama87I | CYCGRG | 3 cut(s) | 240, 836, 3400 |
| Aor13HI | TCCGGA | 2 cut(s) | 731, 2560 |
| AoxI | GGCC | 6 cut(s) | 208, 1375, 2416, 3380, 3764, 4127 |
| ApaLI | GTGCAC | 2 cut(s) | 392, 648 |
| ApeKI | GCWGC | 7 cut(s) | 466, 600, 942, 1016, 1445, 2486, 4181 |
| ApoI | RAATTY | 9 cut(s) | 62, 736, 808, 1345, 1589, 2064, 2386, 2630, 3105 |
| ArsI | GACNNNNNNTTYG | 4 cut(s) | 1691, 1723, 2732, 2764 |
| Asp700I | GAANNNNTTC | 5 cut(s) | 1515, 1887, 2556, 2928, 3800 |
| Asp718I | GGTACC | 1 cut(s) | 4200 |
| AspA2I | CCTAGG | 2 cut(s) | 661, 769 |
| AspLEI | GCGC | 2 cut(s) | 131, 911 |
| AspS9I | GGNCC | 2 cut(s) | 1091, 4127 |
| AsuC2I | CCSGG | 2 cut(s) | 837, 838 |
| AsuII | TTCGAA | 1 cut(s) | 4290 |
| AvaI | CYCGRG | 3 cut(s) | 240, 836, 3400 |
| AvaII | GGWCC | 1 cut(s) | 1091 |
| AvrII | CCTAGG | 2 cut(s) | 661, 769 |
| AxyI | CCTNAGG | 1 cut(s) | 640 |
| BaeGI | GKGCMC | 4 cut(s) | 396, 523, 652, 4114 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 4017, 4050 |
| BamHI | GGATCC | 1 cut(s) | 874 |
| BanI | GGYRCC | 3 cut(s) | 262, 518, 4200 |
| BanII | GRGCYC | 1 cut(s) | 438 |
| BarI | GAAGNNNNNNTAC | 4 cut(s) | 948, 980, 3993, 4025 |
| BbrPI | CACGTG | 1 cut(s) | 479 |
| Bbv12I | GWGCWC | 3 cut(s) | 396, 438, 652 |
| BbvCI | CCTCAGC | 1 cut(s) | 3490 |
| BbvI | GCAGC | 7 cut(s) | 453, 587, 929, 1028, 1457, 2498, 4193 |
| BceAI | ACGGC | 3 cut(s) | 1981, 3022, 3330 |
| BcgI | CGANNNNNNTGC | 8 cut(s) | 1308, 1318, 1342, 1352, 2349, 2359, 2383, 2393 |
| BciVI | GTATCC | 2 cut(s) | 1254, 2295 |
| BclI | TGATCA | 3 cut(s) | 481, 1806, 2847 |
| BcnI | CCSGG | 2 cut(s) | 837, 838 |
| BcoDI | GTCTC | 7 cut(s) | 48, 1392, 1475, 2433, 2516, 3441, 4251 |
| BfmI | CTRYAG | 4 cut(s) | 165, 1197, 2238, 3461 |
| BfrI | CTTAAG | 1 cut(s) | 968 |
| BfuI | GTATCC | 2 cut(s) | 1254, 2295 |
| BglII | AGATCT | 2 cut(s) | 2084, 3125 |
| BisI | GCNGC | 8 cut(s) | 467, 601, 943, 1017, 1446, 2487, 3713, 4182 |
| BlnI | CCTAGG | 2 cut(s) | 661, 769 |
| BlsI | GCNGC | 8 cut(s) | 468, 602, 944, 1018, 1447, 2488, 3714, 4183 |
| Bme18I | GGWCC | 1 cut(s) | 1091 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 4066 |
| BmeT110I | CYCGRG | 3 cut(s) | 240, 836, 3400 |
| BmgBI | CACGTC | 1 cut(s) | 3325 |
| BmgT120I | GGNCC | 2 cut(s) | 1091, 4127 |
| BmiI | GGNNCC | 8 cut(s) | 264, 520, 762, 876, 1546, 2587, 4128, 4202 |
| BmrI | ACTGGG | 2 cut(s) | 577, 4237 |
| BmuI | ACTGGG | 2 cut(s) | 577, 4237 |
| BpmI | CTGGAG | 3 cut(s) | 30, 89, 3315 |
| Bpu10I | CCTNAGC | 1 cut(s) | 3490 |
| Bpu14I | TTCGAA | 1 cut(s) | 4290 |
| BpuEI | CTTGAG | 1 cut(s) | 452 |
| BpuMI | CCSGG | 2 cut(s) | 837, 838 |
| BsaAI | YACGTR | 2 cut(s) | 479, 515 |
| BsaI | GGTCTC | 2 cut(s) | 3441, 4251 |
| BsaWI | WCCGGW | 3 cut(s) | 80, 731, 2560 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 713, 743 |
| Bse118I | RCCGGY | 1 cut(s) | 3328 |
| Bse1I | ACTGG | 9 cut(s) | 508, 572, 1406, 1926, 2447, 2967, 3214, 3697, 4232 |
| Bse21I | CCTNAGG | 1 cut(s) | 640 |
| Bse3DI | GCAATG | 2 cut(s) | 1875, 2916 |
| BseAI | TCCGGA | 2 cut(s) | 731, 2560 |
| BseMI | GCAATG | 2 cut(s) | 1875, 2916 |
| BseMII | CTCAG | 7 cut(s) | 312, 1026, 2553, 3492, 3504, 4004, 4259 |
| BseNI | ACTGG | 9 cut(s) | 508, 572, 1406, 1926, 2447, 2967, 3214, 3697, 4232 |
| BseRI | GAGGAG | 5 cut(s) | 86, 338, 1865, 2906, 3554 |
| BseSI | GKGCMC | 4 cut(s) | 396, 523, 652, 4114 |
| BseXI | GCAGC | 7 cut(s) | 453, 587, 929, 1028, 1457, 2498, 4193 |
| BseYI | CCCAGC | 2 cut(s) | 3438, 3964 |
| BsgI | GTGCAG | 4 cut(s) | 1245, 1368, 2286, 2409 |
| Bsh1236I | CGCG | 2 cut(s) | 1213, 2254 |
| BshFI | GGCC | 6 cut(s) | 210, 1377, 2418, 3382, 3766, 4129 |
| BshNI | GGYRCC | 3 cut(s) | 262, 518, 4200 |
| BsiHKAI | GWGCWC | 3 cut(s) | 396, 438, 652 |
| BsiHKCI | CYCGRG | 3 cut(s) | 240, 836, 3400 |
| BsiSI | CCGG | 6 cut(s) | 81, 457, 732, 837, 2561, 3329 |
| BsmAI | GTCTC | 7 cut(s) | 48, 1392, 1475, 2433, 2516, 3441, 4251 |
| BsmI | GAATGC | 1 cut(s) | 3401 |
| BsnI | GGCC | 6 cut(s) | 210, 1377, 2418, 3382, 3766, 4129 |
| Bso31I | GGTCTC | 2 cut(s) | 3441, 4251 |
| BsoBI | CYCGRG | 3 cut(s) | 240, 836, 3400 |
| Bsp119I | TTCGAA | 1 cut(s) | 4290 |
| Bsp1286I | GDGCHC | 5 cut(s) | 396, 438, 523, 652, 4114 |
| Bsp13I | TCCGGA | 2 cut(s) | 731, 2560 |
| Bsp1407I | TGTACA | 1 cut(s) | 1525 |
| Bsp19I | CCATGG | 1 cut(s) | 4277 |
| BspANI | GGCC | 6 cut(s) | 210, 1377, 2418, 3382, 3766, 4129 |
| BspCNI | CTCAG | 7 cut(s) | 313, 1025, 2552, 3493, 3503, 4005, 4260 |
| BspEI | TCCGGA | 2 cut(s) | 731, 2560 |
| BspFNI | CGCG | 2 cut(s) | 1213, 2254 |
| BspHI | TCATGA | 1 cut(s) | 3514 |
| BspLI | GGNNCC | 8 cut(s) | 264, 520, 762, 876, 1546, 2587, 4128, 4202 |
| BspMAI | CTGCAG | 2 cut(s) | 169, 3465 |
| BspQI | GCTCTTC | 3 cut(s) | 1477, 2518, 4347 |
| BspT104I | TTCGAA | 1 cut(s) | 4290 |
| BspT107I | GGYRCC | 3 cut(s) | 262, 518, 4200 |
| BspTI | CTTAAG | 1 cut(s) | 968 |
| BspTNI | GGTCTC | 2 cut(s) | 3441, 4251 |
| BsrDI | GCAATG | 2 cut(s) | 1875, 2916 |
| BsrFI | RCCGGY | 1 cut(s) | 3328 |
| BsrGI | TGTACA | 1 cut(s) | 1525 |
| BsrI | ACTGG | 9 cut(s) | 508, 572, 1406, 1926, 2447, 2967, 3214, 3697, 4232 |
| BssAI | RCCGGY | 1 cut(s) | 3328 |
| BssNAI | GTATAC | 3 cut(s) | 78, 1051, 1081 |
| BssT1I | CCWWGG | 8 cut(s) | 661, 769, 933, 1825, 1936, 2866, 2977, 4277 |
| Bst1107I | GTATAC | 3 cut(s) | 78, 1051, 1081 |
| Bst6I | CTCTTC | 8 cut(s) | 622, 707, 1224, 1477, 2130, 2265, 2518, 4347 |
| BstAFI | CTTAAG | 1 cut(s) | 968 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 173 |
| BstAUI | TGTACA | 1 cut(s) | 1525 |
| BstBAI | YACGTR | 2 cut(s) | 479, 515 |
| BstBI | TTCGAA | 1 cut(s) | 4290 |
| BstC8I | GCNNGC | 5 cut(s) | 843, 994, 998, 3232, 3330 |
| BstDSI | CCRYGG | 3 cut(s) | 1211, 2252, 4277 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 2135, 3859 |
| BstFNI | CGCG | 2 cut(s) | 1213, 2254 |
| BstHHI | GCGC | 2 cut(s) | 131, 911 |
| BstMAI | GTCTC | 7 cut(s) | 48, 1392, 1475, 2433, 2516, 3441, 4251 |
| BstNSI | RCATGY | 4 cut(s) | 996, 1049, 1726, 2767 |
| BstSFI | CTRYAG | 4 cut(s) | 165, 1197, 2238, 3461 |
| BstSLI | GKGCMC | 4 cut(s) | 396, 523, 652, 4114 |
| BstUI | CGCG | 2 cut(s) | 1213, 2254 |
| BstV1I | GCAGC | 7 cut(s) | 453, 587, 929, 1028, 1457, 2498, 4193 |
| BstX2I | RGATCY | 3 cut(s) | 874, 2084, 3125 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 515, 3160 |
| BstYI | RGATCY | 3 cut(s) | 874, 2084, 3125 |
| BstZ17I | GTATAC | 3 cut(s) | 78, 1051, 1081 |
| Bsu36I | CCTNAGG | 1 cut(s) | 640 |
| BsuI | GTATCC | 2 cut(s) | 1254, 2295 |
| BsuRI | GGCC | 6 cut(s) | 210, 1377, 2418, 3382, 3766, 4129 |
| BtgI | CCRYGG | 3 cut(s) | 1211, 2252, 4277 |
| BtrI | CACGTC | 1 cut(s) | 3325 |
| BtsI | GCAGTG | 1 cut(s) | 3412 |
| BtsIMutI | CAGTG | 8 cut(s) | 33, 342, 565, 1919, 2960, 3207, 3412, 4391 |
| Cac8I | GCNNGC | 5 cut(s) | 843, 994, 998, 3232, 3330 |
| CciI | TCATGA | 1 cut(s) | 3514 |
| CfoI | GCGC | 2 cut(s) | 131, 911 |
| Cfr10I | RCCGGY | 1 cut(s) | 3328 |
| Cfr13I | GGNCC | 2 cut(s) | 1091, 4127 |
| Cfr42I | CCGCGG | 2 cut(s) | 1214, 2255 |
| Cfr9I | CCCGGG | 1 cut(s) | 836 |
| Csp6I | GTAC | 6 cut(s) | 976, 985, 1526, 1777, 2818, 4201 |
| CviQI | GTAC | 6 cut(s) | 976, 985, 1526, 1777, 2818, 4201 |
| DraIII | CACNNNGTG | 1 cut(s) | 3346 |
| DriI | GACNNNNNGTC | 1 cut(s) | 4066 |
| Eam1104I | CTCTTC | 8 cut(s) | 622, 707, 1224, 1477, 2130, 2265, 2518, 4347 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 4066 |
| EarI | CTCTTC | 8 cut(s) | 622, 707, 1224, 1477, 2130, 2265, 2518, 4347 |
| EciI | GGCGGA | 2 cut(s) | 1557, 2598 |
| Ecl136II | GAGCTC | 1 cut(s) | 436 |
| Eco130I | CCWWGG | 8 cut(s) | 661, 769, 933, 1825, 1936, 2866, 2977, 4277 |
| Eco147I | AGGCCT | 4 cut(s) | 210, 1377, 2418, 3382 |
| Eco24I | GRGCYC | 1 cut(s) | 438 |
| Eco31I | GGTCTC | 2 cut(s) | 3441, 4251 |
| Eco32I | GATATC | 7 cut(s) | 318, 1141, 1581, 2182, 2622, 3820, 4165 |
| Eco47I | GGWCC | 1 cut(s) | 1091 |
| Eco53kI | GAGCTC | 1 cut(s) | 436 |
| Eco57I | CTGAAG | 5 cut(s) | 731, 1155, 2196, 4020, 4371 |
| Eco72I | CACGTG | 1 cut(s) | 479 |
| Eco81I | CCTNAGG | 1 cut(s) | 640 |
| Eco88I | CYCGRG | 3 cut(s) | 240, 836, 3400 |
| EcoICRI | GAGCTC | 1 cut(s) | 436 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 2135, 3859 |
| EcoRI | GAATTC | 2 cut(s) | 1345, 2386 |
| EcoRV | GATATC | 7 cut(s) | 318, 1141, 1581, 2182, 2622, 3820, 4165 |
| EcoT14I | CCWWGG | 8 cut(s) | 661, 769, 933, 1825, 1936, 2866, 2977, 4277 |
| EcoT38I | GRGCYC | 1 cut(s) | 438 |
| ErhI | CCWWGG | 8 cut(s) | 661, 769, 933, 1825, 1936, 2866, 2977, 4277 |
| FbaI | TGATCA | 3 cut(s) | 481, 1806, 2847 |
| FblI | GTMKAC | 4 cut(s) | 77, 1050, 1080, 3678 |
| Fnu4HI | GCNGC | 8 cut(s) | 467, 601, 943, 1017, 1446, 2487, 3713, 4182 |
| FriOI | GRGCYC | 1 cut(s) | 438 |
| Fsp4HI | GCNGC | 8 cut(s) | 467, 601, 943, 1017, 1446, 2487, 3713, 4182 |
| GlaI | GCGC | 2 cut(s) | 130, 910 |
| GluI | GCNGC | 8 cut(s) | 467, 601, 943, 1017, 1446, 2487, 3713, 4182 |
| GsaI | CCCAGC | 2 cut(s) | 3442, 3968 |
| GsuI | CTGGAG | 3 cut(s) | 30, 89, 3315 |
| HaeIII | GGCC | 6 cut(s) | 210, 1377, 2418, 3382, 3766, 4129 |
| HapII | CCGG | 6 cut(s) | 81, 457, 732, 837, 2561, 3329 |
| HhaI | GCGC | 2 cut(s) | 131, 911 |
| Hin6I | GCGC | 2 cut(s) | 129, 909 |
| HinP1I | GCGC | 2 cut(s) | 129, 909 |
| HincII | GTYRAC | 3 cut(s) | 1802, 2843, 3679 |
| HindII | GTYRAC | 3 cut(s) | 1802, 2843, 3679 |
| HindIII | AAGCTT | 3 cut(s) | 428, 1036, 3797 |
| HpaII | CCGG | 6 cut(s) | 81, 457, 732, 837, 2561, 3329 |
| Hpy99I | CGWCG | 2 cut(s) | 1330, 2371 |
| HpyAV | CCTTC | 8 cut(s) | 80, 532, 689, 1367, 1877, 2408, 2918, 3865 |
| HpyCH4IV | ACGT | 3 cut(s) | 478, 514, 3324 |
| HpySE526I | ACGT | 3 cut(s) | 478, 514, 3324 |
| HspAI | GCGC | 2 cut(s) | 129, 909 |
| Kpn2I | TCCGGA | 2 cut(s) | 731, 2560 |
| KpnI | GGTACC | 1 cut(s) | 4204 |
| KroI | GCCGGC | 1 cut(s) | 3328 |
| KroNI | GCCGGC | 1 cut(s) | 3330 |
| Ksp22I | TGATCA | 3 cut(s) | 481, 1806, 2847 |
| KspI | CCGCGG | 2 cut(s) | 1214, 2255 |
| LguI | GCTCTTC | 3 cut(s) | 1477, 2518, 4347 |
| LmnI | GCTCC | 6 cut(s) | 441, 631, 766, 1770, 2811, 3666 |
| Lsp1109I | GCAGC | 7 cut(s) | 453, 587, 929, 1028, 1457, 2498, 4193 |
| MaeII | ACGT | 3 cut(s) | 478, 514, 3324 |
| MfeI | CAATTG | 1 cut(s) | 369 |
| MflI | RGATCY | 3 cut(s) | 874, 2084, 3125 |
| MhlI | GDGCHC | 5 cut(s) | 396, 438, 523, 652, 4114 |
| MlyI | GAGTC | 8 cut(s) | 50, 233, 1339, 2380, 3551, 3674, 3785, 4076 |
| MmeI | TCCRAC | 4 cut(s) | 1474, 1712, 2753, 3829 |
| MroI | TCCGGA | 2 cut(s) | 731, 2560 |
| MroNI | GCCGGC | 1 cut(s) | 3328 |
| MroXI | GAANNNNTTC | 5 cut(s) | 1515, 1887, 2556, 2928, 3800 |
| MslI | CAYNNNNRTG | 4 cut(s) | 3390, 3669, 4195, 4386 |
| MspA1I | CMGCKG | 4 cut(s) | 1213, 1879, 2254, 2920 |
| MspCI | CTTAAG | 1 cut(s) | 968 |
| MspI | CCGG | 6 cut(s) | 81, 457, 732, 837, 2561, 3329 |
| MunI | CAATTG | 1 cut(s) | 369 |
| Mva1269I | GAATGC | 1 cut(s) | 3401 |
| MvnI | CGCG | 2 cut(s) | 1213, 2254 |
| NaeI | GCCGGC | 1 cut(s) | 3330 |
| NciI | CCSGG | 2 cut(s) | 837, 838 |
| NcoI | CCATGG | 1 cut(s) | 4277 |
| NgoMIV | GCCGGC | 1 cut(s) | 3328 |
| NlaIV | GGNNCC | 8 cut(s) | 264, 520, 762, 876, 1546, 2587, 4128, 4202 |
| NmuCI | GTSAC | 6 cut(s) | 55, 254, 292, 364, 1418, 2459 |
| NspI | RCATGY | 4 cut(s) | 996, 1049, 1726, 2767 |
| NspV | TTCGAA | 1 cut(s) | 4290 |
| PaeI | GCATGC | 1 cut(s) | 996 |
| PaeR7I | CTCGAG | 1 cut(s) | 240 |
| PagI | TCATGA | 1 cut(s) | 3514 |
| PceI | AGGCCT | 4 cut(s) | 210, 1377, 2418, 3382 |
| PciSI | GCTCTTC | 3 cut(s) | 1477, 2518, 4347 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 1120, 2161 |
| PctI | GAATGC | 1 cut(s) | 3401 |
| PdiI | GCCGGC | 1 cut(s) | 3330 |
| PdmI | GAANNNNTTC | 5 cut(s) | 1515, 1887, 2556, 2928, 3800 |
| PfeI | GAWTC | 7 cut(s) | 1003, 1873, 1888, 2914, 2929, 3454, 3909 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 125, 3346 |
| PkrI | GCNGC | 8 cut(s) | 468, 602, 944, 1018, 1447, 2488, 3714, 4183 |
| PleI | GAGTC | 8 cut(s) | 49, 232, 1338, 2379, 3550, 3674, 3784, 4075 |
| PmaCI | CACGTG | 1 cut(s) | 479 |
| PmlI | CACGTG | 1 cut(s) | 479 |
| PpsI | GAGTC | 8 cut(s) | 49, 232, 1338, 2379, 3550, 3674, 3784, 4075 |
| Ppu21I | YACGTR | 2 cut(s) | 479, 515 |
| PsiI | TTATAA | 4 cut(s) | 534, 864, 1980, 3021 |
| Psp124BI | GAGCTC | 1 cut(s) | 438 |
| PspCI | CACGTG | 1 cut(s) | 479 |
| PspFI | CCCAGC | 2 cut(s) | 3438, 3964 |
| PspN4I | GGNNCC | 8 cut(s) | 264, 520, 762, 876, 1546, 2587, 4128, 4202 |
| PspPI | GGNCC | 2 cut(s) | 1091, 4127 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 1041, 1073 |
| PstI | CTGCAG | 2 cut(s) | 169, 3465 |
| PsuI | RGATCY | 3 cut(s) | 874, 2084, 3125 |
| PvuII | CAGCTG | 2 cut(s) | 1879, 2920 |
| RsaI | GTAC | 6 cut(s) | 977, 986, 1527, 1778, 2819, 4202 |
| RsaNI | GTAC | 6 cut(s) | 976, 985, 1526, 1777, 2818, 4201 |
| RseI | CAYNNNNRTG | 4 cut(s) | 3390, 3669, 4195, 4386 |
| SacI | GAGCTC | 1 cut(s) | 438 |
| SacII | CCGCGG | 2 cut(s) | 1214, 2255 |
| SalI | GTCGAC | 1 cut(s) | 3677 |
| SapI | GCTCTTC | 3 cut(s) | 1477, 2518, 4347 |
| SatI | GCNGC | 8 cut(s) | 467, 601, 943, 1017, 1446, 2487, 3713, 4182 |
| Sau96I | GGNCC | 2 cut(s) | 1091, 4127 |
| SchI | GAGTC | 8 cut(s) | 50, 233, 1339, 2380, 3551, 3674, 3785, 4076 |
| SduI | GDGCHC | 5 cut(s) | 396, 438, 523, 652, 4114 |
| SfcI | CTRYAG | 4 cut(s) | 165, 1197, 2238, 3461 |
| Sfr274I | CTCGAG | 1 cut(s) | 240 |
| Sfr303I | CCGCGG | 2 cut(s) | 1214, 2255 |
| SfuI | TTCGAA | 1 cut(s) | 4290 |
| SgrBI | CCGCGG | 2 cut(s) | 1214, 2255 |
| SinI | GGWCC | 1 cut(s) | 1091 |
| SlaI | CTCGAG | 1 cut(s) | 240 |
| SmaI | CCCGGG | 1 cut(s) | 838 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 3390, 3669, 4195, 4386 |
| SmlI | CTYRAG | 3 cut(s) | 240, 431, 968 |
| SmoI | CTYRAG | 3 cut(s) | 240, 431, 968 |
| SphI | GCATGC | 1 cut(s) | 996 |
| SseBI | AGGCCT | 4 cut(s) | 210, 1377, 2418, 3382 |
| SspI | AATATT | 3 cut(s) | 109, 1553, 2594 |
| SstI | GAGCTC | 1 cut(s) | 438 |
| StuI | AGGCCT | 4 cut(s) | 210, 1377, 2418, 3382 |
| StyI | CCWWGG | 8 cut(s) | 661, 769, 933, 1825, 1936, 2866, 2977, 4277 |
| TaiI | ACGT | 3 cut(s) | 481, 517, 3327 |
| TatI | WGTACW | 3 cut(s) | 975, 984, 1525 |
| TauI | GCSGC | 1 cut(s) | 3715 |
| TfiI | GAWTC | 7 cut(s) | 1003, 1873, 1888, 2914, 2929, 3454, 3909 |
| TscAI | CASTG | 8 cut(s) | 40, 342, 572, 1926, 2967, 3214, 3412, 4391 |
| TseFI | GTSAC | 6 cut(s) | 55, 254, 292, 364, 1418, 2459 |
| TseI | GCWGC | 7 cut(s) | 466, 600, 942, 1016, 1445, 2486, 4181 |
| Tsp45I | GTSAC | 6 cut(s) | 55, 254, 292, 364, 1418, 2459 |
| TspGWI | ACGGA | 3 cut(s) | 397, 582, 2622 |
| TspMI | CCCGGG | 1 cut(s) | 836 |
| TspRI | CASTG | 8 cut(s) | 40, 342, 572, 1926, 2967, 3214, 3412, 4391 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 125, 3346 |
| Vha464I | CTTAAG | 1 cut(s) | 968 |
| VneI | GTGCAC | 2 cut(s) | 392, 648 |
| VpaK11BI | GGWCC | 1 cut(s) | 1091 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 2135, 3859 |
| XapI | RAATTY | 9 cut(s) | 62, 736, 808, 1345, 1589, 2064, 2386, 2630, 3105 |
| XbaI | TCTAGA | 2 cut(s) | 2101, 3142 |
| XceI | RCATGY | 4 cut(s) | 996, 1049, 1726, 2767 |
| XhoI | CTCGAG | 1 cut(s) | 240 |
| XmaI | CCCGGG | 1 cut(s) | 836 |
| XmaJI | CCTAGG | 2 cut(s) | 661, 769 |
| XmiI | GTMKAC | 4 cut(s) | 77, 1050, 1080, 3678 |
| XmnI | GAANNNNTTC | 5 cut(s) | 1515, 1887, 2556, 2928, 3800 |