Probably involved in the RNA silencing pathway and required for the generation of small interfering RNAs (siRNAs)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 588, 2034 |
| Acc36I | ACCTGC | 1 cut(s) | 1818 |
| Acc65I | GGTACC | 2 cut(s) | 1830, 3216 |
| AccB1I | GGYRCC | 3 cut(s) | 572, 1830, 3216 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 125, 2356 |
| AccI | GTMKAC | 4 cut(s) | 77, 1134, 1874, 2728 |
| AccII | CGCG | 2 cut(s) | 1267, 2473 |
| AccIII | TCCGGA | 2 cut(s) | 46, 785 |
| AciI | CCGC | 9 cut(s) | 197, 1265, 1267, 1692, 1705, 2104, 2415, 2471, 2473 |
| AcsI | RAATTY | 6 cut(s) | 62, 155, 790, 862, 2118, 2251 |
| AcuI | CTGAAG | 3 cut(s) | 785, 1209, 3036 |
| AcyI | GRCGYC | 1 cut(s) | 208 |
| AdeI | CACNNNGTG | 1 cut(s) | 2356 |
| AfaI | GTAC | 6 cut(s) | 1031, 1040, 1104, 1832, 2709, 3218 |
| AfiI | CCNNNNNNNGG | 8 cut(s) | 125, 716, 824, 1271, 1996, 2356, 2381, 2702 |
| AflII | CTTAAG | 1 cut(s) | 1022 |
| AflIII | ACRYGT | 1 cut(s) | 289 |
| AhdI | GACNNNNNGTC | 2 cut(s) | 2735, 3082 |
| AhlI | ACTAGT | 1 cut(s) | 2710 |
| AjnI | CCWGG | 5 cut(s) | 966, 1041, 1502, 1529, 1815 |
| AjuI | GAANNNNNNNTTGG | 6 cut(s) | 242, 274, 1595, 1627, 2567, 2599 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1970, 2002 |
| Alw21I | GWGCWC | 3 cut(s) | 396, 438, 706 |
| Alw26I | GTCTC | 4 cut(s) | 48, 1446, 1529, 3267 |
| Alw44I | GTGCAC | 2 cut(s) | 392, 702 |
| Ama87I | CYCGRG | 4 cut(s) | 240, 621, 890, 2302 |
| Aor13HI | TCCGGA | 2 cut(s) | 46, 785 |
| AoxI | GGCC | 3 cut(s) | 1429, 2390, 3143 |
| ApaLI | GTGCAC | 2 cut(s) | 392, 702 |
| ApeKI | GCWGC | 7 cut(s) | 466, 654, 996, 1487, 1499, 2226, 3197 |
| ApoI | RAATTY | 6 cut(s) | 62, 155, 790, 862, 2118, 2251 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1551, 1583 |
| Asp700I | GAANNNNTTC | 4 cut(s) | 1318, 1569, 1941, 2810 |
| Asp718I | GGTACC | 2 cut(s) | 1830, 3216 |
| AspA2I | CCTAGG | 2 cut(s) | 715, 823 |
| AspLEI | GCGC | 1 cut(s) | 131 |
| AspS9I | GGNCC | 3 cut(s) | 1145, 1429, 3143 |
| AsuC2I | CCSGG | 6 cut(s) | 622, 623, 891, 892, 2303, 2304 |
| AsuHPI | GGTGA | 7 cut(s) | 1059, 1361, 1475, 2198, 2833, 3034, 3356 |
| AsuII | TTCGAA | 1 cut(s) | 2928 |
| AvaI | CYCGRG | 4 cut(s) | 240, 621, 890, 2302 |
| AvaII | GGWCC | 1 cut(s) | 1145 |
| AvrII | CCTAGG | 2 cut(s) | 715, 823 |
| BaeGI | GKGCMC | 5 cut(s) | 396, 577, 706, 967, 3130 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 3033, 3066 |
| BamHI | GGATCC | 1 cut(s) | 928 |
| BanI | GGYRCC | 3 cut(s) | 572, 1830, 3216 |
| BanII | GRGCYC | 1 cut(s) | 438 |
| BarI | GAAGNNNNNNTAC | 4 cut(s) | 1417, 1449, 3009, 3041 |
| BbsI | GAAGAC | 2 cut(s) | 773, 1095 |
| Bbv12I | GWGCWC | 3 cut(s) | 396, 438, 706 |
| BbvI | GCAGC | 7 cut(s) | 453, 641, 983, 1499, 1511, 2238, 3209 |
| BciT130I | CCWGG | 5 cut(s) | 968, 1043, 1504, 1531, 1817 |
| BcnI | CCSGG | 6 cut(s) | 622, 623, 891, 892, 2303, 2304 |
| BcoDI | GTCTC | 4 cut(s) | 48, 1446, 1529, 3267 |
| BcuI | ACTAGT | 1 cut(s) | 2710 |
| BfmI | CTRYAG | 1 cut(s) | 1251 |
| BfrI | CTTAAG | 1 cut(s) | 1022 |
| BfuAI | ACCTGC | 1 cut(s) | 1818 |
| BglII | AGATCT | 1 cut(s) | 2138 |
| BisI | GCNGC | 9 cut(s) | 467, 655, 997, 1488, 1500, 2227, 2416, 2474, 3198 |
| BlnI | CCTAGG | 2 cut(s) | 715, 823 |
| BlsI | GCNGC | 9 cut(s) | 468, 656, 998, 1489, 1501, 2228, 2417, 2475, 3199 |
| Bme18I | GGWCC | 1 cut(s) | 1145 |
| BmeRI | GACNNNNNGTC | 2 cut(s) | 2735, 3082 |
| BmeT110I | CYCGRG | 4 cut(s) | 240, 621, 890, 2302 |
| BmgT120I | GGNCC | 3 cut(s) | 1145, 1429, 3143 |
| BmiI | GGNNCC | 7 cut(s) | 574, 816, 930, 1430, 1832, 3144, 3218 |
| BmrI | ACTGGG | 1 cut(s) | 3253 |
| BmuI | ACTGGG | 1 cut(s) | 3253 |
| BoxI | GACNNNNGTC | 1 cut(s) | 3360 |
| BpiI | GAAGAC | 2 cut(s) | 773, 1095 |
| BpmI | CTGGAG | 2 cut(s) | 89, 102 |
| Bpu10I | CCTNAGC | 1 cut(s) | 2410 |
| Bpu14I | TTCGAA | 1 cut(s) | 2928 |
| BpuMI | CCSGG | 6 cut(s) | 622, 623, 891, 892, 2303, 2304 |
| BsaAI | YACGTR | 2 cut(s) | 569, 2726 |
| BsaHI | GRCGYC | 1 cut(s) | 208 |
| BsaI | GGTCTC | 1 cut(s) | 3267 |
| BsaWI | WCCGGW | 2 cut(s) | 46, 785 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 767, 797 |
| Bsc4I | CCNNNNNNNGG | 8 cut(s) | 125, 716, 824, 1271, 1996, 2356, 2381, 2702 |
| Bse1I | ACTGG | 7 cut(s) | 85, 508, 562, 1460, 1980, 2707, 3248 |
| Bse3DI | GCAATG | 2 cut(s) | 1929, 2204 |
| BseAI | TCCGGA | 2 cut(s) | 46, 785 |
| BseBI | CCWGG | 5 cut(s) | 968, 1043, 1504, 1531, 1817 |
| BseLI | CCNNNNNNNGG | 8 cut(s) | 125, 716, 824, 1271, 1996, 2356, 2381, 2702 |
| BseMI | GCAATG | 2 cut(s) | 1929, 2204 |
| BseMII | CTCAG | 5 cut(s) | 312, 1076, 2424, 3020, 3275 |
| BseNI | ACTGG | 7 cut(s) | 85, 508, 562, 1460, 1980, 2707, 3248 |
| BseRI | GAGGAG | 5 cut(s) | 86, 338, 1301, 1306, 2564 |
| BseSI | GKGCMC | 5 cut(s) | 396, 577, 706, 967, 3130 |
| BseXI | GCAGC | 7 cut(s) | 453, 641, 983, 1499, 1511, 2238, 3209 |
| BseYI | CCCAGC | 3 cut(s) | 2448, 2500, 2980 |
| BsgI | GTGCAG | 2 cut(s) | 1299, 1422 |
| Bsh1236I | CGCG | 2 cut(s) | 1267, 2473 |
| BshFI | GGCC | 3 cut(s) | 1431, 2392, 3145 |
| BshNI | GGYRCC | 3 cut(s) | 572, 1830, 3216 |
| BsiHKAI | GWGCWC | 3 cut(s) | 396, 438, 706 |
| BsiHKCI | CYCGRG | 4 cut(s) | 240, 621, 890, 2302 |
| BsiSI | CCGG | 6 cut(s) | 47, 457, 622, 786, 891, 2303 |
| BslFI | GGGAC | 1 cut(s) | 2182 |
| BslI | CCNNNNNNNGG | 8 cut(s) | 125, 716, 824, 1271, 1996, 2356, 2381, 2702 |
| BsmAI | GTCTC | 4 cut(s) | 48, 1446, 1529, 3267 |
| BsmFI | GGGAC | 1 cut(s) | 2182 |
| BsmI | GAATGC | 1 cut(s) | 2411 |
| BsnI | GGCC | 3 cut(s) | 1431, 2392, 3145 |
| Bso31I | GGTCTC | 1 cut(s) | 3267 |
| BsoBI | CYCGRG | 4 cut(s) | 240, 621, 890, 2302 |
| Bsp119I | TTCGAA | 1 cut(s) | 2928 |
| Bsp1286I | GDGCHC | 6 cut(s) | 396, 438, 577, 706, 967, 3130 |
| Bsp13I | TCCGGA | 2 cut(s) | 46, 785 |
| Bsp1407I | TGTACA | 1 cut(s) | 1102 |
| Bsp19I | CCATGG | 2 cut(s) | 1990, 3293 |
| BspACI | CCGC | 9 cut(s) | 197, 1265, 1267, 1692, 1705, 2104, 2415, 2471, 2473 |
| BspANI | GGCC | 3 cut(s) | 1431, 2392, 3145 |
| BspCNI | CTCAG | 5 cut(s) | 313, 1075, 2423, 3021, 3276 |
| BspEI | TCCGGA | 2 cut(s) | 46, 785 |
| BspFNI | CGCG | 2 cut(s) | 1267, 2473 |
| BspLI | GGNNCC | 7 cut(s) | 574, 816, 930, 1430, 1832, 3144, 3218 |
| BspMI | ACCTGC | 1 cut(s) | 1818 |
| BspQI | GCTCTTC | 2 cut(s) | 1531, 2816 |
| BspT104I | TTCGAA | 1 cut(s) | 2928 |
| BspT107I | GGYRCC | 3 cut(s) | 572, 1830, 3216 |
| BspTI | CTTAAG | 1 cut(s) | 1022 |
| BspTNI | GGTCTC | 1 cut(s) | 3267 |
| BsrDI | GCAATG | 2 cut(s) | 1929, 2204 |
| BsrGI | TGTACA | 1 cut(s) | 1102 |
| BsrI | ACTGG | 7 cut(s) | 85, 508, 562, 1460, 1980, 2707, 3248 |
| BssNAI | GTATAC | 2 cut(s) | 78, 1135 |
| BssNI | GRCGYC | 1 cut(s) | 208 |
| BssT1I | CCWWGG | 5 cut(s) | 715, 823, 987, 1990, 3293 |
| Bst1107I | GTATAC | 2 cut(s) | 78, 1135 |
| Bst2UI | CCWGG | 5 cut(s) | 968, 1043, 1504, 1531, 1817 |
| Bst4CI | ACNGT | 8 cut(s) | 13, 37, 94, 756, 978, 2260, 2512, 3132 |
| Bst6I | CTCTTC | 5 cut(s) | 559, 676, 1278, 1531, 2816 |
| BstACI | GRCGYC | 1 cut(s) | 208 |
| BstAFI | CTTAAG | 1 cut(s) | 1022 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 173 |
| BstAUI | TGTACA | 1 cut(s) | 1102 |
| BstBAI | YACGTR | 2 cut(s) | 569, 2726 |
| BstBI | TTCGAA | 1 cut(s) | 2928 |
| BstC8I | GCNNGC | 8 cut(s) | 897, 1048, 1052, 1714, 1761, 1920, 2242, 2340 |
| BstDEI | CTNAG | 7 cut(s) | 321, 1062, 1208, 1328, 2410, 3029, 3284 |
| BstDSI | CCRYGG | 4 cut(s) | 1265, 1990, 2471, 3293 |
| BstFNI | CGCG | 2 cut(s) | 1267, 2473 |
| BstHHI | GCGC | 1 cut(s) | 131 |
| BstMAI | GTCTC | 4 cut(s) | 48, 1446, 1529, 3267 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 173, 1024, 1496, 1713, 1970, 2415, 3203 |
| BstNI | CCWGG | 5 cut(s) | 968, 1043, 1504, 1531, 1817 |
| BstNSI | RCATGY | 4 cut(s) | 293, 1050, 1780, 3313 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 3360 |
| BstSFI | CTRYAG | 1 cut(s) | 1251 |
| BstSLI | GKGCMC | 5 cut(s) | 396, 577, 706, 967, 3130 |
| BstUI | CGCG | 2 cut(s) | 1267, 2473 |
| BstV1I | GCAGC | 7 cut(s) | 453, 641, 983, 1499, 1511, 2238, 3209 |
| BstV2I | GAAGAC | 2 cut(s) | 773, 1095 |
| BstX2I | RGATCY | 2 cut(s) | 928, 2138 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 569 |
| BstYI | RGATCY | 2 cut(s) | 928, 2138 |
| BstZ17I | GTATAC | 2 cut(s) | 78, 1135 |
| BsuRI | GGCC | 3 cut(s) | 1431, 2392, 3145 |
| BtgI | CCRYGG | 4 cut(s) | 1265, 1990, 2471, 3293 |
| BtsI | GCAGTG | 1 cut(s) | 60 |
| BtsIMutI | CAGTG | 4 cut(s) | 33, 60, 1973, 3367 |
| BveI | ACCTGC | 1 cut(s) | 1818 |
| Cac8I | GCNNGC | 8 cut(s) | 897, 1048, 1052, 1714, 1761, 1920, 2242, 2340 |
| CfoI | GCGC | 1 cut(s) | 131 |
| Cfr13I | GGNCC | 3 cut(s) | 1145, 1429, 3143 |
| Cfr42I | CCGCGG | 2 cut(s) | 1268, 2474 |
| Cfr9I | CCCGGG | 3 cut(s) | 621, 890, 2302 |
| CseI | GACGC | 2 cut(s) | 216, 512 |
| Csp6I | GTAC | 6 cut(s) | 1030, 1039, 1103, 1831, 2708, 3217 |
| CviQI | GTAC | 6 cut(s) | 1030, 1039, 1103, 1831, 2708, 3217 |
| DdeI | CTNAG | 7 cut(s) | 321, 1062, 1208, 1328, 2410, 3029, 3284 |
| DraI | TTTAAA | 1 cut(s) | 844 |
| DraIII | CACNNNGTG | 1 cut(s) | 2356 |
| DriI | GACNNNNNGTC | 2 cut(s) | 2735, 3082 |
| Eam1104I | CTCTTC | 5 cut(s) | 559, 676, 1278, 1531, 2816 |
| Eam1105I | GACNNNNNGTC | 2 cut(s) | 2735, 3082 |
| EarI | CTCTTC | 5 cut(s) | 559, 676, 1278, 1531, 2816 |
| EciI | GGCGGA | 1 cut(s) | 1681 |
| Ecl136II | GAGCTC | 1 cut(s) | 436 |
| Eco130I | CCWWGG | 5 cut(s) | 715, 823, 987, 1990, 3293 |
| Eco147I | AGGCCT | 1 cut(s) | 2392 |
| Eco24I | GRGCYC | 1 cut(s) | 438 |
| Eco31I | GGTCTC | 1 cut(s) | 3267 |
| Eco32I | GATATC | 2 cut(s) | 318, 1635 |
| Eco47I | GGWCC | 1 cut(s) | 1145 |
| Eco53kI | GAGCTC | 1 cut(s) | 436 |
| Eco57I | CTGAAG | 3 cut(s) | 785, 1209, 3036 |
| Eco88I | CYCGRG | 4 cut(s) | 240, 621, 890, 2302 |
| EcoICRI | GAGCTC | 1 cut(s) | 436 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1429 |
| EcoRI | GAATTC | 1 cut(s) | 155 |
| EcoRII | CCWGG | 5 cut(s) | 966, 1041, 1502, 1529, 1815 |
| EcoRV | GATATC | 2 cut(s) | 318, 1635 |
| EcoT14I | CCWWGG | 5 cut(s) | 715, 823, 987, 1990, 3293 |
| EcoT38I | GRGCYC | 1 cut(s) | 438 |
| ErhI | CCWWGG | 5 cut(s) | 715, 823, 987, 1990, 3293 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 242, 274 |
| FaqI | GGGAC | 1 cut(s) | 2182 |
| FblI | GTMKAC | 4 cut(s) | 77, 1134, 1874, 2728 |
| Fnu4HI | GCNGC | 9 cut(s) | 467, 655, 997, 1488, 1500, 2227, 2416, 2474, 3198 |
| FriOI | GRGCYC | 1 cut(s) | 438 |
| Fsp4HI | GCNGC | 9 cut(s) | 467, 655, 997, 1488, 1500, 2227, 2416, 2474, 3198 |
| GlaI | GCGC | 1 cut(s) | 130 |
| GluI | GCNGC | 9 cut(s) | 467, 655, 997, 1488, 1500, 2227, 2416, 2474, 3198 |
| GsaI | CCCAGC | 3 cut(s) | 2452, 2504, 2984 |
| GsuI | CTGGAG | 2 cut(s) | 89, 102 |
| HaeIII | GGCC | 3 cut(s) | 1431, 2392, 3145 |
| HapII | CCGG | 6 cut(s) | 47, 457, 622, 786, 891, 2303 |
| HgaI | GACGC | 2 cut(s) | 216, 512 |
| HhaI | GCGC | 1 cut(s) | 131 |
| Hin1I | GRCGYC | 1 cut(s) | 208 |
| Hin6I | GCGC | 1 cut(s) | 129 |
| HinP1I | GCGC | 1 cut(s) | 129 |
| HincII | GTYRAC | 1 cut(s) | 3355 |
| HindII | GTYRAC | 1 cut(s) | 3355 |
| HpaII | CCGG | 6 cut(s) | 47, 457, 622, 786, 891, 2303 |
| HphI | GGTGA | 7 cut(s) | 1059, 1361, 1475, 2198, 2833, 3034, 3356 |
| Hpy99I | CGWCG | 1 cut(s) | 210 |
| HpyAV | CCTTC | 7 cut(s) | 80, 743, 1419, 1931, 2140, 2629, 2881 |
| HpyCH4III | ACNGT | 8 cut(s) | 13, 37, 94, 756, 978, 2260, 2512, 3132 |
| HpyCH4IV | ACGT | 2 cut(s) | 568, 2725 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 173, 1024, 1496, 1713, 1970, 2415, 3203 |
| HpyF3I | CTNAG | 7 cut(s) | 321, 1062, 1208, 1328, 2410, 3029, 3284 |
| HpySE526I | ACGT | 2 cut(s) | 568, 2725 |
| Hsp92I | GRCGYC | 1 cut(s) | 208 |
| HspAI | GCGC | 1 cut(s) | 129 |
| Kpn2I | TCCGGA | 2 cut(s) | 46, 785 |
| KpnI | GGTACC | 2 cut(s) | 1834, 3220 |
| KspI | CCGCGG | 2 cut(s) | 1268, 2474 |
| LguI | GCTCTTC | 2 cut(s) | 1531, 2816 |
| LmnI | GCTCC | 5 cut(s) | 441, 685, 820, 1824, 2676 |
| Lsp1109I | GCAGC | 7 cut(s) | 453, 641, 983, 1499, 1511, 2238, 3209 |
| MaeII | ACGT | 2 cut(s) | 568, 2725 |
| MfeI | CAATTG | 3 cut(s) | 1388, 1856, 1931 |
| MflI | RGATCY | 2 cut(s) | 928, 2138 |
| MhlI | GDGCHC | 6 cut(s) | 396, 438, 577, 706, 967, 3130 |
| MlyI | GAGTC | 5 cut(s) | 50, 233, 2561, 2795, 3092 |
| MmeI | TCCRAC | 2 cut(s) | 1528, 2839 |
| MroI | TCCGGA | 2 cut(s) | 46, 785 |
| MroXI | GAANNNNTTC | 4 cut(s) | 1318, 1569, 1941, 2810 |
| MseI | TTAA | 8 cut(s) | 695, 843, 981, 1023, 1479, 2658, 2859, 3090 |
| MslI | CAYNNNNRTG | 3 cut(s) | 2400, 2679, 3211 |
| MspA1I | CMGCKG | 2 cut(s) | 1267, 2473 |
| MspCI | CTTAAG | 1 cut(s) | 1022 |
| MspI | CCGG | 6 cut(s) | 47, 457, 622, 786, 891, 2303 |
| MunI | CAATTG | 3 cut(s) | 1388, 1856, 1931 |
| Mva1269I | GAATGC | 1 cut(s) | 2411 |
| MvaI | CCWGG | 5 cut(s) | 968, 1043, 1504, 1531, 1817 |
| MvnI | CGCG | 2 cut(s) | 1267, 2473 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 173, 1024, 1496, 1713, 1970, 2415, 3203 |
| NciI | CCSGG | 6 cut(s) | 622, 623, 891, 892, 2303, 2304 |
| NcoI | CCATGG | 2 cut(s) | 1990, 3293 |
| NlaIV | GGNNCC | 7 cut(s) | 574, 816, 930, 1430, 1832, 3144, 3218 |
| NmuCI | GTSAC | 9 cut(s) | 55, 254, 292, 364, 524, 1065, 1472, 2456, 3362 |
| NspI | RCATGY | 4 cut(s) | 293, 1050, 1780, 3313 |
| NspV | TTCGAA | 1 cut(s) | 2928 |
| PaeI | GCATGC | 1 cut(s) | 1050 |
| PaeR7I | CTCGAG | 1 cut(s) | 240 |
| PceI | AGGCCT | 1 cut(s) | 2392 |
| PciI | ACATGT | 1 cut(s) | 289 |
| PciSI | GCTCTTC | 2 cut(s) | 1531, 2816 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 1174, 2731 |
| PctI | GAATGC | 1 cut(s) | 2411 |
| PdmI | GAANNNNTTC | 4 cut(s) | 1318, 1569, 1941, 2810 |
| PfeI | GAWTC | 6 cut(s) | 1057, 1927, 1942, 2464, 2690, 2925 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 125, 2356 |
| PkrI | GCNGC | 9 cut(s) | 468, 656, 998, 1489, 1501, 2228, 2417, 2475, 3199 |
| PleI | GAGTC | 5 cut(s) | 49, 232, 2560, 2794, 3091 |
| PpsI | GAGTC | 5 cut(s) | 49, 232, 2560, 2794, 3091 |
| Ppu21I | YACGTR | 2 cut(s) | 569, 2726 |
| PscI | ACATGT | 1 cut(s) | 289 |
| PshAI | GACNNNNGTC | 1 cut(s) | 3360 |
| PsiI | TTATAA | 2 cut(s) | 588, 2034 |
| Psp124BI | GAGCTC | 1 cut(s) | 438 |
| Psp6I | CCWGG | 5 cut(s) | 966, 1041, 1502, 1529, 1815 |
| PspFI | CCCAGC | 3 cut(s) | 2448, 2500, 2980 |
| PspGI | CCWGG | 5 cut(s) | 966, 1041, 1502, 1529, 1815 |
| PspN4I | GGNNCC | 7 cut(s) | 574, 816, 930, 1430, 1832, 3144, 3218 |
| PspPI | GGNCC | 3 cut(s) | 1145, 1429, 3143 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 1095, 1127 |
| PsuI | RGATCY | 2 cut(s) | 928, 2138 |
| RsaI | GTAC | 6 cut(s) | 1031, 1040, 1104, 1832, 2709, 3218 |
| RsaNI | GTAC | 6 cut(s) | 1030, 1039, 1103, 1831, 2708, 3217 |
| RseI | CAYNNNNRTG | 3 cut(s) | 2400, 2679, 3211 |
| SacI | GAGCTC | 1 cut(s) | 438 |
| SacII | CCGCGG | 2 cut(s) | 1268, 2474 |
| SapI | GCTCTTC | 2 cut(s) | 1531, 2816 |
| SaqAI | TTAA | 8 cut(s) | 695, 843, 981, 1023, 1479, 2658, 2859, 3090 |
| SatI | GCNGC | 9 cut(s) | 467, 655, 997, 1488, 1500, 2227, 2416, 2474, 3198 |
| Sau96I | GGNCC | 3 cut(s) | 1145, 1429, 3143 |
| SchI | GAGTC | 5 cut(s) | 50, 233, 2561, 2795, 3092 |
| SduI | GDGCHC | 6 cut(s) | 396, 438, 577, 706, 967, 3130 |
| SfcI | CTRYAG | 1 cut(s) | 1251 |
| Sfr274I | CTCGAG | 1 cut(s) | 240 |
| Sfr303I | CCGCGG | 2 cut(s) | 1268, 2474 |
| SfuI | TTCGAA | 1 cut(s) | 2928 |
| SgrBI | CCGCGG | 2 cut(s) | 1268, 2474 |
| SinI | GGWCC | 1 cut(s) | 1145 |
| SlaI | CTCGAG | 1 cut(s) | 240 |
| SmaI | CCCGGG | 3 cut(s) | 623, 892, 2304 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 2400, 2679, 3211 |
| SmlI | CTYRAG | 2 cut(s) | 240, 1022 |
| SmoI | CTYRAG | 2 cut(s) | 240, 1022 |
| SpeI | ACTAGT | 1 cut(s) | 2710 |
| SphI | GCATGC | 1 cut(s) | 1050 |
| SseBI | AGGCCT | 1 cut(s) | 2392 |
| SsiI | CCGC | 9 cut(s) | 197, 1265, 1267, 1692, 1705, 2104, 2415, 2471, 2473 |
| SspI | AATATT | 2 cut(s) | 1607, 2933 |
| SstI | GAGCTC | 1 cut(s) | 438 |
| StuI | AGGCCT | 1 cut(s) | 2392 |
| StyI | CCWWGG | 5 cut(s) | 715, 823, 987, 1990, 3293 |
| TaaI | ACNGT | 8 cut(s) | 13, 37, 94, 756, 978, 2260, 2512, 3132 |
| TaiI | ACGT | 2 cut(s) | 571, 2728 |
| TaqI | TCGA | 7 cut(s) | 205, 241, 628, 2928, 3086, 3138, 3306 |
| TatI | WGTACW | 2 cut(s) | 1102, 2707 |
| TauI | GCSGC | 2 cut(s) | 2418, 2476 |
| TfiI | GAWTC | 6 cut(s) | 1057, 1927, 1942, 2464, 2690, 2925 |
| Tru1I | TTAA | 8 cut(s) | 695, 843, 981, 1023, 1479, 2658, 2859, 3090 |
| Tru9I | TTAA | 8 cut(s) | 695, 843, 981, 1023, 1479, 2658, 2859, 3090 |
| TscAI | CASTG | 3 cut(s) | 40, 60, 1980 |
| TseFI | GTSAC | 9 cut(s) | 55, 254, 292, 364, 524, 1065, 1472, 2456, 3362 |
| TseI | GCWGC | 7 cut(s) | 466, 654, 996, 1487, 1499, 2226, 3197 |
| Tsp45I | GTSAC | 9 cut(s) | 55, 254, 292, 364, 524, 1065, 1472, 2456, 3362 |
| TspMI | CCCGGG | 3 cut(s) | 621, 890, 2302 |
| TspRI | CASTG | 3 cut(s) | 40, 60, 1980 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 125, 2356 |
| Vha464I | CTTAAG | 1 cut(s) | 1022 |
| VneI | GTGCAC | 2 cut(s) | 392, 702 |
| VpaK11BI | GGWCC | 1 cut(s) | 1145 |
| XapI | RAATTY | 6 cut(s) | 62, 155, 790, 862, 2118, 2251 |
| XceI | RCATGY | 4 cut(s) | 293, 1050, 1780, 3313 |
| XhoI | CTCGAG | 1 cut(s) | 240 |
| XmaI | CCCGGG | 3 cut(s) | 621, 890, 2302 |
| XmaJI | CCTAGG | 2 cut(s) | 715, 823 |
| XmiI | GTMKAC | 4 cut(s) | 77, 1134, 1874, 2728 |
| XmnI | GAANNNNTTC | 4 cut(s) | 1318, 1569, 1941, 2810 |