RchiOBHm_Chr5g0043091

DUF21 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0001961
Physical Location & Seq
Reverse (-)
37945445 .. 37952228
6784 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ32142

Sequence Viewer

Length: 576 bp
ATGGATCTCCTGGATTATGTTCAGAAGATGGATTCAATGTCAGTCTGGAATCTTATTAGAGAATTCCGCATTTGGAAGGTTCACATGACTGTGGTTCTCAATGAATATGGTGGAACTGTAGGGATAATTACCCTACAAGATGTGGTGGAGGAAATTGTTGGTAAAATATTTGATGAAAATGATTCCAAGGAGGTGATTCAGAAAAAAGGGTACATCGTAATGCGTGCAGAGGGAGTATTTGATGTGGATGCAAACACATCTATAGACCAGCTCTCTGAAGATCTAAATGTGAAAATGCCTGAGGTGCGTGAGTTAGCTGAGAAAGAGCCTGAAAATGGCGTCTTCGGATTGCTTCGCAATGATGTGACTCGGTTCTTGGCAACCATACTTGTTGGCACAACTGTTGTCAATATTGGAGCAACTGCTTTAGTTACAGAGGCTGCAACAGCGATATTTGGTGAAGCTGGTGTTAGTGCAGCAACTGGAGTAATGACTGTTCTTATTTTGCTCCTCACGAAAATAACTCCCAAAAGTGGTGCTGTTCACAATCCCACAGAAGTTGCTAGGTTTGTGTGA

Protein Analysis

191

Amino Acids

20.94

Weight (kDa)

4.68

Isoelectric Point (pI)

29.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CNNM PF01595 97 - 191 4.4e-18 Cyclin M transmembrane N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 67
AclWI GGATC 1 cut(s) 12
AcsI RAATTY 1 cut(s) 62
AcuI CTGAAG 1 cut(s) 297
AcyI GRCGYC 1 cut(s) 339
AfaI GTAC 1 cut(s) 212
AfiI CCNNNNNNNGG 2 cut(s) 335, 533
AgsI TTSAA 1 cut(s) 36
AjnI CCWGG 1 cut(s) 9
AluBI AGCT 3 cut(s) 271, 317, 464
AluI AGCT 3 cut(s) 271, 317, 464
AlwI GGATC 1 cut(s) 12
AlwNI CAGNNNCTG 2 cut(s) 440, 482
ApeKI GCWGC 2 cut(s) 440, 476
ApoI RAATTY 1 cut(s) 62
AsuHPI GGTGA 2 cut(s) 205, 470
AxyI CCTNAGG 1 cut(s) 300
BbsI GAAGAC 1 cut(s) 334
BbvI GCAGC 2 cut(s) 427, 488
BccI CCATC 1 cut(s) 22
BciT130I CCWGG 1 cut(s) 11
BfaI CTAG 1 cut(s) 564
BfmI CTRYAG 2 cut(s) 117, 261
BglII AGATCT 1 cut(s) 280
BisI GCNGC 2 cut(s) 441, 477
BlsI GCNGC 2 cut(s) 442, 478
Bme1390I CCNGG 1 cut(s) 11
BmrFI CCNGG 1 cut(s) 11
BmsI GCATC 1 cut(s) 238
BpiI GAAGAC 1 cut(s) 334
BpmI CTGGAG 1 cut(s) 504
BsaHI GRCGYC 1 cut(s) 339
BsaJI CCNNGG 1 cut(s) 186
Bsc4I CCNNNNNNNGG 2 cut(s) 335, 533
Bse1I ACTGG 1 cut(s) 487
Bse21I CCTNAGG 1 cut(s) 300
Bse3DI GCAATG 1 cut(s) 364
BseBI CCWGG 1 cut(s) 11
BseDI CCNNGG 1 cut(s) 186
BseGI GGATG 1 cut(s) 253
BseLI CCNNNNNNNGG 2 cut(s) 335, 533
BseMI GCAATG 1 cut(s) 364
BseMII CTCAG 2 cut(s) 291, 309
BseNI ACTGG 1 cut(s) 487
BseRI GAGGAG 1 cut(s) 500
BseXI GCAGC 2 cut(s) 427, 488
BsgI GTGCAG 2 cut(s) 246, 495
BslI CCNNNNNNNGG 2 cut(s) 335, 533
Bsp143I GATC 2 cut(s) 4, 280
BspACI CCGC 1 cut(s) 67
BspCNI CTCAG 2 cut(s) 292, 310
BspPI GGATC 1 cut(s) 12
BsrDI GCAATG 1 cut(s) 364
BsrI ACTGG 1 cut(s) 487
BssECI CCNNGG 1 cut(s) 186
BssMI GATC 2 cut(s) 4, 280
BssNI GRCGYC 1 cut(s) 339
BssT1I CCWWGG 1 cut(s) 186
Bst2UI CCWGG 1 cut(s) 11
Bst4CI ACNGT 4 cut(s) 91, 118, 403, 496
BstACI GRCGYC 1 cut(s) 339
BstC8I GCNNGC 1 cut(s) 225
BstDEI CTNAG 2 cut(s) 300, 318
BstF5I GGATG 1 cut(s) 253
BstKTI GATC 2 cut(s) 7, 283
BstMBI GATC 2 cut(s) 4, 280
BstMWI GCNNNNNNNGC 2 cut(s) 304, 446
BstNI CCWGG 1 cut(s) 11
BstSCI CCNGG 1 cut(s) 9
BstSFI CTRYAG 2 cut(s) 117, 261
BstV1I GCAGC 2 cut(s) 427, 488
BstV2I GAAGAC 1 cut(s) 334
BstX2I RGATCY 2 cut(s) 4, 280
BstYI RGATCY 2 cut(s) 4, 280
Bsu36I CCTNAGG 1 cut(s) 300
BtsCI GGATG 1 cut(s) 253
Cac8I GCNNGC 1 cut(s) 225
CaiI CAGNNNCTG 2 cut(s) 440, 482
CseI GACGC 1 cut(s) 328
Csp6I GTAC 1 cut(s) 211
CspCI CAANNNNNGTGG 2 cut(s) 541, 576
CviAII CATG 1 cut(s) 85
CviJI RGCY 5 cut(s) 271, 317, 328, 440, 464
CviKI_1 RGCY 5 cut(s) 271, 317, 328, 440, 464
CviQI GTAC 1 cut(s) 211
DdeI CTNAG 2 cut(s) 300, 318
DpnI GATC 2 cut(s) 6, 282
DpnII GATC 2 cut(s) 4, 280
Eco130I CCWWGG 1 cut(s) 186
Eco57I CTGAAG 1 cut(s) 297
Eco81I CCTNAGG 1 cut(s) 300
EcoRI GAATTC 1 cut(s) 62
EcoRII CCWGG 1 cut(s) 9
EcoT14I CCWWGG 1 cut(s) 186
ErhI CCWWGG 1 cut(s) 186
FaeI CATG 1 cut(s) 88
FaiI YATR 5 cut(s) 18, 86, 108, 263, 386
FatI CATG 1 cut(s) 84
Fnu4HI GCNGC 2 cut(s) 441, 477
FokI GGATG 1 cut(s) 260
Fsp4HI GCNGC 2 cut(s) 441, 477
FspBI CTAG 1 cut(s) 564
GluI GCNGC 2 cut(s) 441, 477
GsuI CTGGAG 1 cut(s) 504
HgaI GACGC 1 cut(s) 328
Hin1I GRCGYC 1 cut(s) 339
Hin1II CATG 1 cut(s) 88
HinfI GANTC 5 cut(s) 32, 49, 182, 196, 367
HphI GGTGA 2 cut(s) 205, 470
Hpy166II GTNNAC 2 cut(s) 82, 544
Hpy188I TCNGA 4 cut(s) 24, 201, 277, 347
Hpy188III TCNNGA 2 cut(s) 46, 514
Hpy8I GTNNAC 2 cut(s) 82, 544
HpyAV CCTTC 1 cut(s) 70
HpyCH4III ACNGT 4 cut(s) 91, 118, 403, 496
HpyCH4V TGCA 4 cut(s) 227, 251, 443, 476
HpyF10VI GCNNNNNNNGC 2 cut(s) 304, 446
HpyF3I CTNAG 2 cut(s) 300, 318
Hsp92I GRCGYC 1 cut(s) 339
Hsp92II CATG 1 cut(s) 88
Kzo9I GATC 2 cut(s) 4, 280
LmnI GCTCC 2 cut(s) 416, 513
LpnPI CCDG 7 cut(s) 23, 31, 281, 312, 342, 450, 468
Lsp1109I GCAGC 2 cut(s) 427, 488
LweI GCATC 1 cut(s) 238
MaeI CTAG 1 cut(s) 564
MaeIII GTNAC 2 cut(s) 364, 430
MalI GATC 2 cut(s) 6, 282
MboI GATC 2 cut(s) 4, 280
MboII GAAGA 3 cut(s) 37, 290, 334
MflI RGATCY 2 cut(s) 4, 280
MluCI AATT 3 cut(s) 62, 126, 153
MlyI GAGTC 1 cut(s) 361
MnlI CCTC 6 cut(s) 142, 184, 223, 295, 430, 521
MslI CAYNNNNRTG 2 cut(s) 89, 218
MspR9I CCNGG 1 cut(s) 11
MvaI CCWGG 1 cut(s) 11
MwoI GCNNNNNNNGC 2 cut(s) 304, 446
NdeII GATC 2 cut(s) 4, 280
NlaIII CATG 1 cut(s) 88
NmuCI GTSAC 1 cut(s) 364
PfeI GAWTC 4 cut(s) 32, 49, 182, 196
PfoI TCCNGGA 1 cut(s) 9
PkrI GCNGC 2 cut(s) 442, 478
PleI GAGTC 1 cut(s) 361
PpsI GAGTC 1 cut(s) 361
Psp6I CCWGG 1 cut(s) 9
PspGI CCWGG 1 cut(s) 9
PsrI GAACNNNNNNTAC 2 cut(s) 480, 512
PstNI CAGNNNCTG 2 cut(s) 440, 482
PsuI RGATCY 2 cut(s) 4, 280
RsaI GTAC 1 cut(s) 212
RsaNI GTAC 1 cut(s) 211
RseI CAYNNNNRTG 2 cut(s) 89, 218
SatI GCNGC 2 cut(s) 441, 477
Sau3AI GATC 2 cut(s) 4, 280
SchI GAGTC 1 cut(s) 361
ScrFI CCNGG 1 cut(s) 11
SetI ASST 7 cut(s) 81, 195, 273, 306, 319, 466, 569
SfaNI GCATC 1 cut(s) 238
SfcI CTRYAG 2 cut(s) 117, 261
SmiMI CAYNNNNRTG 2 cut(s) 89, 218
Sse9I AATT 3 cut(s) 62, 126, 153
SsiI CCGC 1 cut(s) 67
SspI AATATT 2 cut(s) 168, 412
SspMI CTAG 1 cut(s) 564
StyD4I CCNGG 1 cut(s) 9
StyI CCWWGG 1 cut(s) 186
TaaI ACNGT 4 cut(s) 91, 118, 403, 496
TasI AATT 3 cut(s) 62, 126, 153
TfiI GAWTC 4 cut(s) 32, 49, 182, 196
TseFI GTSAC 1 cut(s) 364
TseI GCWGC 2 cut(s) 440, 476
Tsp45I GTSAC 1 cut(s) 364
TspDTI ATGAA 2 cut(s) 117, 189
XapI RAATTY 1 cut(s) 62
XspI CTAG 1 cut(s) 564
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.