RLG00000034159

DUF21 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0001961
Physical Location & Seq
Reverse (-)
37523314 .. 37524601
1288 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000034159

Sequence Viewer

Length: 696 bp
ATGGCGCTTGACTCTTCAATTCTAAGCCCACCCAGATTCATTTCTAGTTCAAAATCAATGATTTTCTTGTACTACCATAAGCAGAAGAATTTCCCCACCAAGGTTTTGGTACAAAATAATCTATACCCATTTCGATTGGTATCGAATTGCATTGGCAATAGCGATTTTGGGAGGTTCTTAGGTCCAAGATGCGAGGGAGTTGACGTGTTTGCTAATAGAGCTCGTTTGAGAACTGGAGGTAATGATAGTGAGAGCTCTAGTGGTACAAATGCTGTTTCGGGTTTGAGTTTTGATTTTGTTAGGGTAATGGGGAAGTGTGGATTTGTTTTAGCAGCTGTGGTTTGTGGGGTTTGTACGTGGAGAGCTTTTGCTGTGGAGGGTGTTGTCAATGCAAGCTATGGGCTTTTTTCTCAATGGCAGAGACTTCGATTACCTCGCTATGGCCTTGGAAGGCACGAACTTTGTGTTTTCGCCTTGTGGGTGCGTGAGTTGGCTGAGAAGGAGCCTGAAAATGGTGTCTTCGGATTGCTTCGCTATGATGTGACTCGGTTCTTGACAACCATACTTATTGGCACAACTGTTGTCAATATTGGAGCAACTGCTTTAGTTACAGAGGCTGCAACAACGATATTTGGTGAAGTTGGTGTTAGTTCAGCCACTGGAGTAACGACTGTTTGTCTCATTTTCCCTGCCTGA

Protein Analysis

232

Amino Acids

25.18

Weight (kDa)

9.06

Isoelectric Point (pI)

32.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CNNM PF01595 162 - 228 1.1e-08 Cyclin M transmembrane N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 88
AfaI GTAC 4 cut(s) 71, 111, 265, 355
AfiI CCNNNNNNNGG 3 cut(s) 100, 440, 512
AflIII ACRYGT 1 cut(s) 204
AgsI TTSAA 2 cut(s) 18, 51
AhdI GACNNNNNGTC 1 cut(s) 675
AjiI CACGTC 1 cut(s) 205
AluBI AGCT 5 cut(s) 221, 255, 335, 365, 396
AluI AGCT 5 cut(s) 221, 255, 335, 365, 396
Alw21I GWGCWC 2 cut(s) 223, 257
Alw26I GTCTC 2 cut(s) 415, 683
AlwNI CAGNNNCTG 2 cut(s) 617, 659
AoxI GGCC 1 cut(s) 442
ApeKI GCWGC 2 cut(s) 332, 617
ApoI RAATTY 1 cut(s) 88
Asp700I GAANNNNTTC 1 cut(s) 89
AspLEI GCGC 1 cut(s) 7
AspS9I GGNCC 1 cut(s) 182
AsuHPI GGTGA 1 cut(s) 647
AvaII GGWCC 1 cut(s) 182
BanII GRGCYC 2 cut(s) 223, 257
BbsI GAAGAC 1 cut(s) 511
Bbv12I GWGCWC 2 cut(s) 223, 257
BbvI GCAGC 2 cut(s) 344, 604
BcgI CGANNNNNNTGC 2 cut(s) 407, 441
BcoDI GTCTC 2 cut(s) 415, 683
BfaI CTAG 2 cut(s) 45, 258
BfoI RGCGCY 1 cut(s) 8
BisI GCNGC 2 cut(s) 333, 618
BlsI GCNGC 2 cut(s) 334, 619
Bme18I GGWCC 1 cut(s) 182
BmeRI GACNNNNNGTC 1 cut(s) 675
BmgBI CACGTC 1 cut(s) 205
BmgT120I GGNCC 1 cut(s) 182
BmiI GGNNCC 1 cut(s) 504
BmsI GCATC 1 cut(s) 179
BpiI GAAGAC 1 cut(s) 511
BpmI CTGGAG 2 cut(s) 255, 681
BsaAI YACGTR 1 cut(s) 357
BsaBI GATNNNNATC 1 cut(s) 139
BsaJI CCNNGG 2 cut(s) 99, 445
BsaXI ACNNNNNCTCC 2 cut(s) 189, 219
Bsc4I CCNNNNNNNGG 3 cut(s) 100, 440, 512
Bse1I ACTGG 2 cut(s) 238, 664
Bse8I GATNNNNATC 1 cut(s) 139
BseDI CCNNGG 2 cut(s) 99, 445
BseJI GATNNNNATC 1 cut(s) 139
BseLI CCNNNNNNNGG 3 cut(s) 100, 440, 512
BseMII CTCAG 1 cut(s) 486
BseNI ACTGG 2 cut(s) 238, 664
BseXI GCAGC 2 cut(s) 344, 604
BshFI GGCC 1 cut(s) 444
BsiHKAI GWGCWC 2 cut(s) 223, 257
BslI CCNNNNNNNGG 3 cut(s) 100, 440, 512
BsmAI GTCTC 2 cut(s) 415, 683
BsnI GGCC 1 cut(s) 444
Bsp1286I GDGCHC 2 cut(s) 223, 257
BspANI GGCC 1 cut(s) 444
BspCNI CTCAG 1 cut(s) 487
BspLI GGNNCC 1 cut(s) 504
BsrI ACTGG 2 cut(s) 238, 664
BssECI CCNNGG 2 cut(s) 99, 445
BssT1I CCWWGG 2 cut(s) 99, 445
Bst4CI ACNGT 2 cut(s) 580, 673
Bst6I CTCTTC 1 cut(s) 19
BstBAI YACGTR 1 cut(s) 357
BstC8I GCNNGC 1 cut(s) 394
BstDEI CTNAG 3 cut(s) 23, 178, 495
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 1 cut(s) 7
BstMAI GTCTC 2 cut(s) 415, 683
BstMWI GCNNNNNNNGC 1 cut(s) 218
BstV1I GCAGC 2 cut(s) 344, 604
BstV2I GAAGAC 1 cut(s) 511
BstXI CCANNNNNNTGG 1 cut(s) 106
BsuRI GGCC 1 cut(s) 444
BtrI CACGTC 1 cut(s) 205
BtsIMutI CAGTG 1 cut(s) 657
Cac8I GCNNGC 1 cut(s) 394
CaiI CAGNNNCTG 2 cut(s) 617, 659
CfoI GCGC 1 cut(s) 7
Cfr13I GGNCC 1 cut(s) 182
Csp6I GTAC 4 cut(s) 70, 110, 264, 354
CviQI GTAC 4 cut(s) 70, 110, 264, 354
DdeI CTNAG 3 cut(s) 23, 178, 495
DriI GACNNNNNGTC 1 cut(s) 675
Eam1104I CTCTTC 1 cut(s) 19
Eam1105I GACNNNNNGTC 1 cut(s) 675
EarI CTCTTC 1 cut(s) 19
Ecl136II GAGCTC 2 cut(s) 221, 255
Eco130I CCWWGG 2 cut(s) 99, 445
Eco24I GRGCYC 2 cut(s) 223, 257
Eco47I GGWCC 1 cut(s) 182
Eco53kI GAGCTC 2 cut(s) 221, 255
EcoICRI GAGCTC 2 cut(s) 221, 255
EcoT14I CCWWGG 2 cut(s) 99, 445
EcoT38I GRGCYC 2 cut(s) 223, 257
ErhI CCWWGG 2 cut(s) 99, 445
FaiI YATR 6 cut(s) 78, 124, 399, 441, 537, 563
Fnu4HI GCNGC 2 cut(s) 333, 618
FriOI GRGCYC 2 cut(s) 223, 257
Fsp4HI GCNGC 2 cut(s) 333, 618
FspBI CTAG 2 cut(s) 45, 258
GlaI GCGC 1 cut(s) 6
GluI GCNGC 2 cut(s) 333, 618
GsuI CTGGAG 2 cut(s) 255, 681
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 1 cut(s) 444
HhaI GCGC 1 cut(s) 7
Hin6I GCGC 1 cut(s) 5
HinP1I GCGC 1 cut(s) 5
HincII GTYRAC 1 cut(s) 202
HindII GTYRAC 1 cut(s) 202
HinfI GANTC 3 cut(s) 11, 36, 544
HphI GGTGA 1 cut(s) 647
Hpy166II GTNNAC 1 cut(s) 202
Hpy188I TCNGA 1 cut(s) 524
Hpy188III TCNNGA 1 cut(s) 553
Hpy8I GTNNAC 1 cut(s) 202
HpyAV CCTTC 2 cut(s) 444, 493
HpyCH4III ACNGT 2 cut(s) 580, 673
HpyCH4IV ACGT 2 cut(s) 204, 356
HpyCH4V TGCA 3 cut(s) 150, 392, 620
HpyF10VI GCNNNNNNNGC 1 cut(s) 218
HpyF3I CTNAG 3 cut(s) 23, 178, 495
HpySE526I ACGT 2 cut(s) 204, 356
HspAI GCGC 1 cut(s) 5
LmnI GCTCC 2 cut(s) 502, 593
LpnPI CCDG 4 cut(s) 46, 219, 519, 645
Lsp1109I GCAGC 2 cut(s) 344, 604
LweI GCATC 1 cut(s) 179
MaeI CTAG 2 cut(s) 45, 258
MaeII ACGT 2 cut(s) 204, 356
MaeIII GTNAC 3 cut(s) 541, 607, 664
MboII GAAGA 3 cut(s) 6, 97, 511
MhlI GDGCHC 2 cut(s) 223, 257
MluCI AATT 3 cut(s) 18, 88, 145
MlyI GAGTC 2 cut(s) 5, 538
MnlI CCTC 6 cut(s) 165, 187, 230, 370, 444, 607
MroXI GAANNNNTTC 1 cut(s) 89
MspA1I CMGCKG 1 cut(s) 335
MwoI GCNNNNNNNGC 1 cut(s) 218
NlaIV GGNNCC 1 cut(s) 504
NmuCI GTSAC 1 cut(s) 541
PdmI GAANNNNTTC 1 cut(s) 89
PfeI GAWTC 1 cut(s) 36
PkrI GCNGC 2 cut(s) 334, 619
PleI GAGTC 2 cut(s) 5, 538
PpsI GAGTC 2 cut(s) 5, 538
Ppu21I YACGTR 1 cut(s) 357
Psp124BI GAGCTC 2 cut(s) 223, 257
PspN4I GGNNCC 1 cut(s) 504
PspPI GGNCC 1 cut(s) 182
PstNI CAGNNNCTG 2 cut(s) 617, 659
PvuII CAGCTG 1 cut(s) 335
RsaI GTAC 4 cut(s) 71, 111, 265, 355
RsaNI GTAC 4 cut(s) 70, 110, 264, 354
SacI GAGCTC 2 cut(s) 223, 257
SatI GCNGC 2 cut(s) 333, 618
Sau96I GGNCC 1 cut(s) 182
SchI GAGTC 2 cut(s) 5, 538
SduI GDGCHC 2 cut(s) 223, 257
SfaNI GCATC 1 cut(s) 179
SinI GGWCC 1 cut(s) 182
Sse9I AATT 3 cut(s) 18, 88, 145
SspI AATATT 1 cut(s) 589
SspMI CTAG 2 cut(s) 45, 258
SstI GAGCTC 2 cut(s) 223, 257
StyI CCWWGG 2 cut(s) 99, 445
TaaI ACNGT 2 cut(s) 580, 673
TaiI ACGT 2 cut(s) 207, 359
TaqI TCGA 3 cut(s) 133, 143, 427
TasI AATT 3 cut(s) 18, 88, 145
TatI WGTACW 1 cut(s) 69
TfiI GAWTC 1 cut(s) 36
TscAI CASTG 1 cut(s) 664
TseFI GTSAC 1 cut(s) 541
TseI GCWGC 2 cut(s) 332, 617
Tsp45I GTSAC 1 cut(s) 541
TspDTI ATGAA 1 cut(s) 28
TspRI CASTG 1 cut(s) 664
VpaK11BI GGWCC 1 cut(s) 182
XapI RAATTY 1 cut(s) 88
XcmI CCANNNNNNNNNTGG 1 cut(s) 103
XmnI GAANNNNTTC 1 cut(s) 89
XspI CTAG 2 cut(s) 45, 258
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.