RLG00000010835

Auxilin-like protein 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0002396
Physical Location & Seq
Reverse (-)
4679290 .. 4680137
848 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000010835

Sequence Viewer

Length: 423 bp
ATGTGTCCGACAAAGCAGAAGCACCGGAGTTCCGGAGTCAATCACGCTGCAAATGCTCTAGATCCGGAGAAGCCCGATGAAGATGAAGACGAAGAAGAAAACAAATCTTCGCTGGAGGGGAACAGGCTTCGCGCCTCCTCAATTTCGGTGCTTGAGGTCCCCGTCGCCGCTGTCGCCGGTGACAATGAGGAGTTCTTCGATGTGCACGGCTTCGGCTTCGACTACTTCGAGGTCTTTGGAGGCTTCAACGACCTGGATTTCACCGTCGCGTACGACAACCTAGTCAAGCAACCTTATGGCGGAGACGATGGTTCCTTTGACGAAGCTTGGACTCTCAGCAGAATCTGGGTCTCTCTCAATAAAGTCGAATCATTCTGTGAAGAACCAAAGCTTTACAGATGGAGATCCTTACTATTCATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

141

Amino Acids

15.85

Weight (kDa)

4.4

Isoelectric Point (pI)

53.72

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 281
AccII CGCG 2 cut(s) 132, 269
AccIII TCCGGA 2 cut(s) 32, 64
AciI CCGC 2 cut(s) 168, 300
AclWI GGATC 2 cut(s) 56, 399
AfaI GTAC 1 cut(s) 272
AfiI CCNNNNNNNGG 1 cut(s) 299
AgsI TTSAA 1 cut(s) 247
AjnI CCWGG 1 cut(s) 252
AluBI AGCT 2 cut(s) 326, 391
AluI AGCT 2 cut(s) 326, 391
Alw21I GWGCWC 1 cut(s) 207
Alw26I GTCTC 2 cut(s) 297, 355
Alw44I GTGCAC 1 cut(s) 203
AlwI GGATC 2 cut(s) 56, 399
AlwNI CAGNNNCTG 1 cut(s) 345
Aor13HI TCCGGA 2 cut(s) 32, 64
ApaLI GTGCAC 1 cut(s) 203
ApeKI GCWGC 1 cut(s) 47
AspLEI GCGC 1 cut(s) 134
AspS9I GGNCC 1 cut(s) 157
AsuHPI GGTGA 2 cut(s) 191, 253
AvaII GGWCC 1 cut(s) 157
BaeGI GKGCMC 1 cut(s) 207
BbsI GAAGAC 1 cut(s) 93
Bbv12I GWGCWC 1 cut(s) 207
BbvI GCAGC 1 cut(s) 34
BccI CCATC 2 cut(s) 302, 393
BceAI ACGGC 1 cut(s) 223
BciT130I CCWGG 1 cut(s) 254
BcoDI GTCTC 2 cut(s) 297, 355
BfaI CTAG 2 cut(s) 59, 281
BisI GCNGC 2 cut(s) 48, 168
BlsI GCNGC 2 cut(s) 49, 169
Bme1390I CCNGG 1 cut(s) 254
Bme18I GGWCC 1 cut(s) 157
BmgT120I GGNCC 1 cut(s) 157
BmiI GGNNCC 2 cut(s) 159, 313
BmrFI CCNGG 1 cut(s) 254
BpiI GAAGAC 1 cut(s) 93
BpmI CTGGAG 1 cut(s) 134
BpuEI CTTGAG 1 cut(s) 173
BsaBI GATNNNNATC 1 cut(s) 403
BsaI GGTCTC 1 cut(s) 355
BsaWI WCCGGW 3 cut(s) 24, 32, 64
Bsc4I CCNNNNNNNGG 1 cut(s) 299
Bse118I RCCGGY 1 cut(s) 176
Bse8I GATNNNNATC 1 cut(s) 403
BseAI TCCGGA 2 cut(s) 32, 64
BseBI CCWGG 1 cut(s) 254
BseJI GATNNNNATC 1 cut(s) 403
BseLI CCNNNNNNNGG 1 cut(s) 299
BseMII CTCAG 1 cut(s) 349
BseRI GAGGAG 2 cut(s) 127, 203
BseSI GKGCMC 1 cut(s) 207
BseXI GCAGC 1 cut(s) 34
Bsh1236I CGCG 2 cut(s) 132, 269
BsiHKAI GWGCWC 1 cut(s) 207
BsiSI CCGG 4 cut(s) 25, 33, 65, 177
BsiWI CGTACG 1 cut(s) 270
BslFI GGGAC 1 cut(s) 143
BslI CCNNNNNNNGG 1 cut(s) 299
BsmAI GTCTC 2 cut(s) 297, 355
BsmBI CGTCTC 1 cut(s) 297
BsmFI GGGAC 1 cut(s) 143
Bso31I GGTCTC 1 cut(s) 355
Bsp1286I GDGCHC 1 cut(s) 207
Bsp13I TCCGGA 2 cut(s) 32, 64
Bsp143I GATC 2 cut(s) 61, 404
BspACI CCGC 2 cut(s) 168, 300
BspCNI CTCAG 1 cut(s) 348
BspEI TCCGGA 2 cut(s) 32, 64
BspFNI CGCG 2 cut(s) 132, 269
BspLI GGNNCC 2 cut(s) 159, 313
BspPI GGATC 2 cut(s) 56, 399
BspTNI GGTCTC 1 cut(s) 355
BsrFI RCCGGY 1 cut(s) 176
BssAI RCCGGY 1 cut(s) 176
BssMI GATC 2 cut(s) 61, 404
Bst2UI CCWGG 1 cut(s) 254
Bst4CI ACNGT 1 cut(s) 265
BstDEI CTNAG 1 cut(s) 335
BstFNI CGCG 2 cut(s) 132, 269
BstHHI GCGC 1 cut(s) 134
BstKTI GATC 2 cut(s) 64, 407
BstMAI GTCTC 2 cut(s) 297, 355
BstMBI GATC 2 cut(s) 61, 404
BstMWI GCNNNNNNNGC 2 cut(s) 53, 173
BstNI CCWGG 1 cut(s) 254
BstSCI CCNGG 1 cut(s) 252
BstSLI GKGCMC 1 cut(s) 207
BstUI CGCG 2 cut(s) 132, 269
BstV1I GCAGC 1 cut(s) 34
BstV2I GAAGAC 1 cut(s) 93
BstX2I RGATCY 2 cut(s) 61, 404
BstYI RGATCY 2 cut(s) 61, 404
CaiI CAGNNNCTG 1 cut(s) 345
CfoI GCGC 1 cut(s) 134
Cfr10I RCCGGY 1 cut(s) 176
Cfr13I GGNCC 1 cut(s) 157
Csp6I GTAC 1 cut(s) 271
CviJI RGCY 7 cut(s) 73, 127, 210, 216, 243, 326, 391
CviKI_1 RGCY 7 cut(s) 73, 127, 210, 216, 243, 326, 391
CviQI GTAC 1 cut(s) 271
DdeI CTNAG 1 cut(s) 335
DpnI GATC 2 cut(s) 63, 406
DpnII GATC 2 cut(s) 61, 404
DrdI GACNNNNNNGTC 1 cut(s) 281
DseDI GACNNNNNNGTC 1 cut(s) 281
EciI GGCGGA 1 cut(s) 315
Eco31I GGTCTC 1 cut(s) 355
Eco47I GGWCC 1 cut(s) 157
EcoO109I RGGNCCY 1 cut(s) 157
EcoRII CCWGG 1 cut(s) 252
Esp3I CGTCTC 1 cut(s) 297
FaiI YATR 1 cut(s) 297
FaqI GGGAC 1 cut(s) 143
Fnu4HI GCNGC 2 cut(s) 48, 168
Fsp4HI GCNGC 2 cut(s) 48, 168
FspBI CTAG 2 cut(s) 59, 281
GlaI GCGC 1 cut(s) 133
GluI GCNGC 2 cut(s) 48, 168
GsuI CTGGAG 1 cut(s) 134
HapII CCGG 4 cut(s) 25, 33, 65, 177
HhaI GCGC 1 cut(s) 134
Hin6I GCGC 1 cut(s) 132
HinP1I GCGC 1 cut(s) 132
HindIII AAGCTT 2 cut(s) 324, 389
HinfI GANTC 4 cut(s) 36, 331, 342, 368
HpaII CCGG 4 cut(s) 25, 33, 65, 177
HphI GGTGA 2 cut(s) 191, 253
Hpy166II GTNNAC 1 cut(s) 205
Hpy188I TCNGA 1 cut(s) 9
Hpy188III TCNNGA 3 cut(s) 33, 59, 65
Hpy8I GTNNAC 1 cut(s) 205
Hpy99I CGWCG 2 cut(s) 167, 269
HpyCH4III ACNGT 1 cut(s) 265
HpyCH4V TGCA 2 cut(s) 50, 205
HpyF10VI GCNNNNNNNGC 2 cut(s) 53, 173
HpyF3I CTNAG 1 cut(s) 335
HspAI GCGC 1 cut(s) 132
Kpn2I TCCGGA 2 cut(s) 32, 64
Kzo9I GATC 2 cut(s) 61, 404
LpnPI CCDG 9 cut(s) 38, 46, 78, 98, 109, 190, 239, 266, 331
Lsp1109I GCAGC 1 cut(s) 34
MaeI CTAG 2 cut(s) 59, 281
MaeIII GTNAC 1 cut(s) 179
MalI GATC 2 cut(s) 63, 406
MboI GATC 2 cut(s) 61, 404
MboII GAAGA 7 cut(s) 92, 98, 99, 104, 107, 187, 392
MflI RGATCY 2 cut(s) 61, 404
MhlI GDGCHC 1 cut(s) 207
MluCI AATT 1 cut(s) 141
MlyI GAGTC 2 cut(s) 45, 325
MmeI TCCRAC 1 cut(s) 32
MnlI CCTC 7 cut(s) 109, 145, 148, 148, 181, 223, 233
MroI TCCGGA 2 cut(s) 32, 64
MspA1I CMGCKG 1 cut(s) 170
MspI CCGG 4 cut(s) 25, 33, 65, 177
MspR9I CCNGG 1 cut(s) 254
MvaI CCWGG 1 cut(s) 254
MvnI CGCG 2 cut(s) 132, 269
MwoI GCNNNNNNNGC 2 cut(s) 53, 173
NdeII GATC 2 cut(s) 61, 404
NlaIV GGNNCC 2 cut(s) 159, 313
NmuCI GTSAC 1 cut(s) 179
PcsI WCGNNNNNNNCGW 1 cut(s) 225
PfeI GAWTC 2 cut(s) 342, 368
Pfl23II CGTACG 1 cut(s) 270
PkrI GCNGC 2 cut(s) 49, 169
PleI GAGTC 2 cut(s) 44, 325
PpsI GAGTC 2 cut(s) 44, 325
PpuMI RGGWCCY 1 cut(s) 157
Psp5II RGGWCCY 1 cut(s) 157
Psp6I CCWGG 1 cut(s) 252
PspGI CCWGG 1 cut(s) 252
PspLI CGTACG 1 cut(s) 270
PspN4I GGNNCC 2 cut(s) 159, 313
PspPI GGNCC 1 cut(s) 157
PspPPI RGGWCCY 1 cut(s) 157
PstNI CAGNNNCTG 1 cut(s) 345
PsuI RGATCY 2 cut(s) 61, 404
RsaI GTAC 1 cut(s) 272
RsaNI GTAC 1 cut(s) 271
SatI GCNGC 2 cut(s) 48, 168
Sau3AI GATC 2 cut(s) 61, 404
Sau96I GGNCC 1 cut(s) 157
SchI GAGTC 2 cut(s) 45, 325
ScrFI CCNGG 1 cut(s) 254
SduI GDGCHC 1 cut(s) 207
SetI ASST 7 cut(s) 159, 234, 255, 282, 295, 328, 393
SgrAI CRCCGGYG 1 cut(s) 176
SinI GGWCC 1 cut(s) 157
SmlI CTYRAG 1 cut(s) 152
SmoI CTYRAG 1 cut(s) 152
Sse9I AATT 1 cut(s) 141
SsiI CCGC 2 cut(s) 168, 300
SspMI CTAG 2 cut(s) 59, 281
StyD4I CCNGG 1 cut(s) 252
TaaI ACNGT 1 cut(s) 265
TaqI TCGA 4 cut(s) 198, 219, 228, 366
TasI AATT 1 cut(s) 141
TauI GCSGC 1 cut(s) 170
TfiI GAWTC 2 cut(s) 342, 368
TseFI GTSAC 1 cut(s) 179
TseI GCWGC 1 cut(s) 47
Tsp45I GTSAC 1 cut(s) 179
TspDTI ATGAA 3 cut(s) 93, 99, 406
VneI GTGCAC 1 cut(s) 203
VpaK11BI GGWCC 1 cut(s) 157
XbaI TCTAGA 1 cut(s) 58
XspI CTAG 2 cut(s) 59, 281
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.