Rmu_sc0010612.1_g000001

Auxilin-like protein 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0010612.1
OG0002396
Physical Location & Seq
Forward (+)
1 .. 1513
1513 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0010612.1_g000001.1.cds

Sequence Viewer

Length: 598 bp
acaaatgctccagatccggaaagtccacaacctccaccaccaattggattttcaagacaatccaaggatgatccttaccccacgtcaaccttcacactggaacgaacggctgggcttggcagagaacttggcgcttggcgccgaggaagacaatgatggcgggtggtgcctcaaccaagagcccgataaagatgaagacaaagaagaaaacgattctccgctggaggggaataggcttcgtgcctcctcaattcccgtgctcgaggtccctgtcgccgtcgacaacgacaatgaggagttcttcaatgtgcacgactccgacttcaactactccgaggtcttcggatgcttcaacggcctagattccgccgtcgagtacgacaaccttgtcaagcaaccctacggcggagacggtggagagtcaaatcattatgtgaagaacaaaagctttacagatggagatccttactattcatttgatggcagctctaagtttgacatatcctataacaaagctcttaaaaaaagacatcaggaggaagaagcggggaaggttgcagaggagatggagggaggtttctggagaagagagagctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

198

Amino Acids

22.65

Weight (kDa)

4.56

Isoelectric Point (pI)

57.59

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 387
AccB1I GGYRCC 2 cut(s) 138, 166
AccB7I CCANNNNNTGG 1 cut(s) 44
AccI GTMKAC 1 cut(s) 280
AccIII TCCGGA 1 cut(s) 16
AciI CCGC 5 cut(s) 160, 219, 367, 406, 546
AclWI GGATC 3 cut(s) 8, 65, 456
AcyI GRCGYC 1 cut(s) 139
AfaI GTAC 1 cut(s) 378
AfiI CCNNNNNNNGG 3 cut(s) 44, 225, 405
AgsI TTSAA 4 cut(s) 54, 305, 326, 353
AjiI CACGTC 1 cut(s) 84
AjuI GAANNNNNNNTTGG 2 cut(s) 34, 66
AluBI AGCT 4 cut(s) 448, 487, 516, 595
AluI AGCT 4 cut(s) 448, 487, 516, 595
Alw21I GWGCWC 2 cut(s) 262, 313
Alw26I GTCTC 1 cut(s) 403
Alw44I GTGCAC 1 cut(s) 309
AlwI GGATC 3 cut(s) 8, 65, 456
Ama87I CYCGRG 1 cut(s) 261
Aor13HI TCCGGA 1 cut(s) 16
AoxI GGCC 1 cut(s) 356
ApaLI GTGCAC 1 cut(s) 309
ApeKI GCWGC 1 cut(s) 484
AspLEI GCGC 2 cut(s) 134, 141
AspS9I GGNCC 1 cut(s) 266
AvaI CYCGRG 1 cut(s) 261
AvaII GGWCC 1 cut(s) 266
BaeGI GKGCMC 1 cut(s) 313
BanI GGYRCC 2 cut(s) 138, 166
BanII GRGCYC 1 cut(s) 184
BbsI GAAGAC 3 cut(s) 154, 202, 332
Bbv12I GWGCWC 2 cut(s) 262, 313
BbvI GCAGC 1 cut(s) 496
BccI CCATC 4 cut(s) 150, 450, 474, 560
BceAI ACGGC 5 cut(s) 123, 261, 354, 371, 419
BcoDI GTCTC 1 cut(s) 403
BfaI CTAG 1 cut(s) 360
BfoI RGCGCY 2 cut(s) 135, 142
BisI GCNGC 1 cut(s) 485
BlsI GCNGC 1 cut(s) 486
Bme18I GGWCC 1 cut(s) 266
BmeT110I CYCGRG 1 cut(s) 261
BmgBI CACGTC 1 cut(s) 84
BmgT120I GGNCC 1 cut(s) 266
BmiI GGNNCC 3 cut(s) 140, 168, 268
BmsI GCATC 1 cut(s) 336
BpiI GAAGAC 3 cut(s) 154, 202, 332
BpmI CTGGAG 1 cut(s) 243
BsaBI GATNNNNATC 1 cut(s) 460
BsaHI GRCGYC 1 cut(s) 139
BsaJI CCNNGG 3 cut(s) 63, 142, 334
BsaWI WCCGGW 1 cut(s) 16
BsaXI ACNNNNNCTCC 1 cut(s) 22
Bsc4I CCNNNNNNNGG 3 cut(s) 44, 225, 405
Bse1I ACTGG 1 cut(s) 102
Bse8I GATNNNNATC 1 cut(s) 460
BseAI TCCGGA 1 cut(s) 16
BseDI CCNNGG 3 cut(s) 63, 142, 334
BseGI GGATG 2 cut(s) 73, 351
BseJI GATNNNNATC 1 cut(s) 460
BseLI CCNNNNNNNGG 3 cut(s) 44, 225, 405
BseNI ACTGG 1 cut(s) 102
BseRI GAGGAG 3 cut(s) 236, 309, 576
BseSI GKGCMC 1 cut(s) 313
BseXI GCAGC 1 cut(s) 496
BseYI CCCAGC 1 cut(s) 110
BshFI GGCC 1 cut(s) 358
BshNI GGYRCC 2 cut(s) 138, 166
BsiHKAI GWGCWC 2 cut(s) 262, 313
BsiHKCI CYCGRG 1 cut(s) 261
BsiSI CCGG 1 cut(s) 17
BslFI GGGAC 1 cut(s) 252
BslI CCNNNNNNNGG 3 cut(s) 44, 225, 405
BsmAI GTCTC 1 cut(s) 403
BsmBI CGTCTC 1 cut(s) 403
BsmFI GGGAC 1 cut(s) 252
BsnI GGCC 1 cut(s) 358
BsoBI CYCGRG 1 cut(s) 261
Bsp1286I GDGCHC 3 cut(s) 184, 262, 313
Bsp13I TCCGGA 1 cut(s) 16
Bsp143I GATC 3 cut(s) 13, 70, 461
BspACI CCGC 5 cut(s) 160, 219, 367, 406, 546
BspANI GGCC 1 cut(s) 358
BspEI TCCGGA 1 cut(s) 16
BspLI GGNNCC 3 cut(s) 140, 168, 268
BspPI GGATC 3 cut(s) 8, 65, 456
BspT107I GGYRCC 2 cut(s) 138, 166
BsrI ACTGG 1 cut(s) 102
BssECI CCNNGG 3 cut(s) 63, 142, 334
BssMI GATC 3 cut(s) 13, 70, 461
BssNI GRCGYC 1 cut(s) 139
BssT1I CCWWGG 1 cut(s) 63
Bst4CI ACNGT 1 cut(s) 414
Bst6I CTCTTC 1 cut(s) 581
BstACI GRCGYC 1 cut(s) 139
BstDEI CTNAG 1 cut(s) 490
BstF5I GGATG 2 cut(s) 73, 351
BstH2I RGCGCY 2 cut(s) 135, 142
BstHHI GCGC 2 cut(s) 134, 141
BstKTI GATC 3 cut(s) 16, 73, 464
BstMAI GTCTC 1 cut(s) 403
BstMBI GATC 3 cut(s) 13, 70, 461
BstMWI GCNNNNNNNGC 3 cut(s) 138, 166, 355
BstSLI GKGCMC 1 cut(s) 313
BstV1I GCAGC 1 cut(s) 496
BstV2I GAAGAC 3 cut(s) 154, 202, 332
BstX2I RGATCY 2 cut(s) 13, 461
BstYI RGATCY 2 cut(s) 13, 461
BsuRI GGCC 1 cut(s) 358
BtrI CACGTC 1 cut(s) 84
BtsCI GGATG 2 cut(s) 73, 351
BtsIMutI CAGTG 1 cut(s) 95
CfoI GCGC 2 cut(s) 134, 141
Cfr13I GGNCC 1 cut(s) 266
Csp6I GTAC 1 cut(s) 377
CviJI RGCY 9 cut(s) 110, 115, 182, 236, 358, 448, 487, 516, 595
CviKI_1 RGCY 9 cut(s) 110, 115, 182, 236, 358, 448, 487, 516, 595
CviQI GTAC 1 cut(s) 377
DdeI CTNAG 1 cut(s) 490
DinI GGCGCC 1 cut(s) 140
DpnI GATC 3 cut(s) 15, 72, 463
DpnII GATC 3 cut(s) 13, 70, 461
DrdI GACNNNNNNGTC 1 cut(s) 387
DseDI GACNNNNNNGTC 1 cut(s) 387
Eam1104I CTCTTC 1 cut(s) 581
EarI CTCTTC 1 cut(s) 581
EciI GGCGGA 2 cut(s) 356, 421
Eco130I CCWWGG 1 cut(s) 63
Eco24I GRGCYC 1 cut(s) 184
Eco47I GGWCC 1 cut(s) 266
Eco88I CYCGRG 1 cut(s) 261
EcoO109I RGGNCCY 1 cut(s) 266
EcoT14I CCWWGG 1 cut(s) 63
EcoT38I GRGCYC 1 cut(s) 184
EgeI GGCGCC 1 cut(s) 140
EheI GGCGCC 1 cut(s) 140
ErhI CCWWGG 1 cut(s) 63
Esp3I CGTCTC 1 cut(s) 403
FaiI YATR 3 cut(s) 433, 501, 508
FaqI GGGAC 1 cut(s) 252
FauI CCCGC 2 cut(s) 153, 539
FblI GTMKAC 1 cut(s) 280
Fnu4HI GCNGC 1 cut(s) 485
FokI GGATG 2 cut(s) 80, 358
FriOI GRGCYC 1 cut(s) 184
Fsp4HI GCNGC 1 cut(s) 485
FspBI CTAG 1 cut(s) 360
GlaI GCGC 2 cut(s) 133, 140
GluI GCNGC 1 cut(s) 485
GsaI CCCAGC 1 cut(s) 114
GsuI CTGGAG 1 cut(s) 243
HaeII RGCGCY 2 cut(s) 135, 142
HaeIII GGCC 1 cut(s) 358
HapII CCGG 1 cut(s) 17
HhaI GCGC 2 cut(s) 134, 141
Hin1I GRCGYC 1 cut(s) 139
Hin6I GCGC 2 cut(s) 132, 139
HinP1I GCGC 2 cut(s) 132, 139
HincII GTYRAC 2 cut(s) 87, 281
HindII GTYRAC 2 cut(s) 87, 281
HindIII AAGCTT 1 cut(s) 446
HinfI GANTC 4 cut(s) 213, 315, 363, 420
HpaII CCGG 1 cut(s) 17
Hpy166II GTNNAC 4 cut(s) 26, 87, 281, 311
Hpy188I TCNGA 3 cut(s) 320, 335, 345
Hpy188III TCNNGA 5 cut(s) 11, 17, 54, 534, 581
Hpy8I GTNNAC 4 cut(s) 26, 87, 281, 311
Hpy99I CGWCG 2 cut(s) 282, 375
HpyAV CCTTC 2 cut(s) 100, 545
HpyCH4III ACNGT 1 cut(s) 414
HpyCH4IV ACGT 1 cut(s) 83
HpyCH4V TGCA 2 cut(s) 311, 558
HpyF10VI GCNNNNNNNGC 3 cut(s) 138, 166, 355
HpyF3I CTNAG 1 cut(s) 490
HpySE526I ACGT 1 cut(s) 83
Hsp92I GRCGYC 1 cut(s) 139
HspAI GCGC 2 cut(s) 132, 139
KasI GGCGCC 1 cut(s) 138
Kpn2I TCCGGA 1 cut(s) 16
Kzo9I GATC 3 cut(s) 13, 70, 461
LmnI GCTCC 1 cut(s) 13
LpnPI CCDG 8 cut(s) 24, 30, 83, 96, 207, 283, 519, 566
Lsp1109I GCAGC 1 cut(s) 496
LweI GCATC 1 cut(s) 336
MaeI CTAG 1 cut(s) 360
MaeII ACGT 1 cut(s) 83
MalI GATC 3 cut(s) 15, 72, 463
MboI GATC 3 cut(s) 13, 70, 461
MboII GAAGA 8 cut(s) 159, 207, 216, 293, 332, 449, 552, 598
MfeI CAATTG 1 cut(s) 42
MflI RGATCY 2 cut(s) 13, 461
MhlI GDGCHC 3 cut(s) 184, 262, 313
MluCI AATT 2 cut(s) 42, 250
Mly113I GGCGCC 1 cut(s) 139
MlyI GAGTC 2 cut(s) 309, 429
MmeI TCCRAC 1 cut(s) 343
MroI TCCGGA 1 cut(s) 16
MseI TTAA 1 cut(s) 520
MspA1I CMGCKG 1 cut(s) 221
MspI CCGG 1 cut(s) 17
MunI CAATTG 1 cut(s) 42
MwoI GCNNNNNNNGC 3 cut(s) 138, 166, 355
NarI GGCGCC 1 cut(s) 139
NdeII GATC 3 cut(s) 13, 70, 461
NlaIV GGNNCC 3 cut(s) 140, 168, 268
NmeAIII GCCGAG 1 cut(s) 167
PaeR7I CTCGAG 1 cut(s) 261
PfeI GAWTC 2 cut(s) 213, 363
PflMI CCANNNNNTGG 1 cut(s) 44
PkrI GCNGC 1 cut(s) 486
PleI GAGTC 2 cut(s) 309, 428
PluTI GGCGCC 1 cut(s) 142
PpsI GAGTC 2 cut(s) 309, 428
PpuMI RGGWCCY 1 cut(s) 266
Psp5II RGGWCCY 1 cut(s) 266
PspFI CCCAGC 1 cut(s) 110
PspN4I GGNNCC 3 cut(s) 140, 168, 268
PspPI GGNCC 1 cut(s) 266
PspPPI RGGWCCY 1 cut(s) 266
PspXI VCTCGAGB 1 cut(s) 261
PsuI RGATCY 2 cut(s) 13, 461
RsaI GTAC 1 cut(s) 378
RsaNI GTAC 1 cut(s) 377
SalI GTCGAC 1 cut(s) 279
SaqAI TTAA 1 cut(s) 520
SatI GCNGC 1 cut(s) 485
Sau3AI GATC 3 cut(s) 13, 70, 461
Sau96I GGNCC 1 cut(s) 266
SchI GAGTC 2 cut(s) 309, 429
SduI GDGCHC 3 cut(s) 184, 262, 313
SfaNI GCATC 1 cut(s) 336
SfoI GGCGCC 1 cut(s) 140
Sfr274I CTCGAG 1 cut(s) 261
SinI GGWCC 1 cut(s) 266
SlaI CTCGAG 1 cut(s) 261
SmlI CTYRAG 1 cut(s) 261
SmoI CTYRAG 1 cut(s) 261
Sse9I AATT 2 cut(s) 42, 250
SsiI CCGC 5 cut(s) 160, 219, 367, 406, 546
SspDI GGCGCC 1 cut(s) 138
SspMI CTAG 1 cut(s) 360
StyI CCWWGG 1 cut(s) 63
TaaI ACNGT 1 cut(s) 414
TaiI ACGT 1 cut(s) 86
TaqI TCGA 3 cut(s) 262, 280, 373
TasI AATT 2 cut(s) 42, 250
TfiI GAWTC 2 cut(s) 213, 363
Tru1I TTAA 1 cut(s) 520
Tru9I TTAA 1 cut(s) 520
TscAI CASTG 1 cut(s) 102
TseI GCWGC 1 cut(s) 484
TspDTI ATGAA 2 cut(s) 208, 463
TspRI CASTG 1 cut(s) 102
Van91I CCANNNNNTGG 1 cut(s) 44
VneI GTGCAC 1 cut(s) 309
VpaK11BI GGWCC 1 cut(s) 266
XhoI CTCGAG 1 cut(s) 261
XmiI GTMKAC 1 cut(s) 280
XspI CTAG 1 cut(s) 360
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.