RLG00000016048

B3 domain-containing transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0002871
Physical Location & Seq
Forward (+)
3483764 .. 3485012
1249 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000016048

Sequence Viewer

Length: 528 bp
ATGGTCTTGGAGTACATTGGACGTGCAAAGTTCAATGTTGAAATTTATGGGCTCAATCACTGCAATAAGATGTTAAAGGCTAGGAAGAGTGACAGGCTTCTTGATGATCATGATGTGATTATTGTGGACTCTGATTCAGATTCTGAAATTGAGTTTCCTAGGGAACCGGTTTGTGGAAGAAAATTATCAAGGAGAGGTAGACCTCGTAATCTAGCCACCGTAAACCGTTCCGGAAATCCTTGCTTCAGTGTCCAGATGTGCCAGACTCATGTGACCAAGGGACCATTGCTCACACCGAGGAGCTTCATGAGGAACACACACAAGCCAAAGAAAGTTGAGCTTCAAGTTGGAGACATGGTCTGGGATGTGAACTGGATCCATCCTTACCAGAAGTCTAGTAGGTTCAGTGGAGGCTGGCTACGTTTTGTGAGCCATAACGGATTGCAAGAGGGAGATTTCTGTGACTTTGAGCTAATATCGAAGACAAATGAGGTTGCGGTGATGAACGTTTGTATTAGGAAAATGTAG

Protein Analysis

176

Amino Acids

20.2

Weight (kDa)

9.23

Isoelectric Point (pI)

40.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 84 - 170 1.5e-12 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 199
AccIII TCCGGA 1 cut(s) 230
AciI CCGC 1 cut(s) 497
AclI AACGTT 1 cut(s) 507
AclWI GGATC 2 cut(s) 370, 383
AcsI RAATTY 1 cut(s) 42
AcuI CTGAAG 1 cut(s) 229
AfaI GTAC 1 cut(s) 14
AfiI CCNNNNNNNGG 1 cut(s) 173
AgeI ACCGGT 1 cut(s) 166
AgsI TTSAA 3 cut(s) 34, 41, 344
AjiI CACGTC 1 cut(s) 23
AluBI AGCT 3 cut(s) 303, 340, 472
AluI AGCT 3 cut(s) 303, 340, 472
Alw26I GTCTC 1 cut(s) 345
AlwI GGATC 2 cut(s) 370, 383
AlwNI CAGNNNCTG 1 cut(s) 143
Aor13HI TCCGGA 1 cut(s) 230
ApoI RAATTY 1 cut(s) 42
AsiGI ACCGGT 1 cut(s) 166
AspA2I CCTAGG 1 cut(s) 158
AspS9I GGNCC 1 cut(s) 281
AsuHPI GGTGA 1 cut(s) 511
AvaII GGWCC 1 cut(s) 281
AvrII CCTAGG 1 cut(s) 158
BamHI GGATCC 1 cut(s) 375
BanII GRGCYC 1 cut(s) 54
BbsI GAAGAC 1 cut(s) 488
BccI CCATC 1 cut(s) 387
BclI TGATCA 1 cut(s) 106
BcoDI GTCTC 1 cut(s) 345
BfaI CTAG 4 cut(s) 81, 159, 212, 396
BlnI CCTAGG 1 cut(s) 158
Bme18I GGWCC 1 cut(s) 281
BmgBI CACGTC 1 cut(s) 23
BmgT120I GGNCC 1 cut(s) 281
BmiI GGNNCC 3 cut(s) 165, 282, 377
BpiI GAAGAC 1 cut(s) 488
BsaJI CCNNGG 3 cut(s) 158, 276, 296
BsaWI WCCGGW 2 cut(s) 166, 230
BsaXI ACNNNNNCTCC 2 cut(s) 342, 372
Bsc4I CCNNNNNNNGG 1 cut(s) 173
Bse118I RCCGGY 1 cut(s) 166
Bse1I ACTGG 1 cut(s) 377
Bse3DI GCAATG 1 cut(s) 284
BseAI TCCGGA 1 cut(s) 230
BseDI CCNNGG 3 cut(s) 158, 276, 296
BseGI GGATG 2 cut(s) 370, 379
BseLI CCNNNNNNNGG 1 cut(s) 173
BseMI GCAATG 1 cut(s) 284
BseNI ACTGG 1 cut(s) 377
BseRI GAGGAG 1 cut(s) 313
BshTI ACCGGT 1 cut(s) 166
BsiSI CCGG 2 cut(s) 167, 231
BslFI GGGAC 1 cut(s) 294
BslI CCNNNNNNNGG 1 cut(s) 173
BsmAI GTCTC 1 cut(s) 345
BsmFI GGGAC 1 cut(s) 294
Bsp1286I GDGCHC 1 cut(s) 54
Bsp13I TCCGGA 1 cut(s) 230
Bsp143I GATC 2 cut(s) 106, 375
BspACI CCGC 1 cut(s) 497
BspEI TCCGGA 1 cut(s) 230
BspHI TCATGA 2 cut(s) 109, 306
BspLI GGNNCC 3 cut(s) 165, 282, 377
BspPI GGATC 2 cut(s) 370, 383
BsrDI GCAATG 1 cut(s) 284
BsrFI RCCGGY 1 cut(s) 166
BsrI ACTGG 1 cut(s) 377
BssAI RCCGGY 1 cut(s) 166
BssECI CCNNGG 3 cut(s) 158, 276, 296
BssMI GATC 2 cut(s) 106, 375
BssT1I CCWWGG 2 cut(s) 158, 276
Bst4CI ACNGT 2 cut(s) 220, 227
Bst6I CTCTTC 1 cut(s) 80
BstC8I GCNNGC 1 cut(s) 416
BstF5I GGATG 2 cut(s) 370, 379
BstKTI GATC 2 cut(s) 109, 378
BstMAI GTCTC 1 cut(s) 345
BstMBI GATC 2 cut(s) 106, 375
BstV2I GAAGAC 1 cut(s) 488
BstX2I RGATCY 1 cut(s) 375
BstYI RGATCY 1 cut(s) 375
BtrI CACGTC 1 cut(s) 23
BtsCI GGATG 2 cut(s) 370, 379
BtsI GCAGTG 1 cut(s) 58
BtsIMutI CAGTG 3 cut(s) 58, 253, 412
Cac8I GCNNGC 1 cut(s) 416
CaiI CAGNNNCTG 1 cut(s) 143
CciI TCATGA 2 cut(s) 109, 306
Cfr10I RCCGGY 1 cut(s) 166
Cfr13I GGNCC 1 cut(s) 281
Csp6I GTAC 1 cut(s) 13
CspAI ACCGGT 1 cut(s) 166
CviAII CATG 4 cut(s) 110, 269, 307, 355
CviQI GTAC 1 cut(s) 13
DpnI GATC 2 cut(s) 108, 377
DpnII GATC 2 cut(s) 106, 375
Eam1104I CTCTTC 1 cut(s) 80
EarI CTCTTC 1 cut(s) 80
Eco130I CCWWGG 2 cut(s) 158, 276
Eco24I GRGCYC 1 cut(s) 54
Eco47I GGWCC 1 cut(s) 281
Eco57I CTGAAG 1 cut(s) 229
EcoT14I CCWWGG 2 cut(s) 158, 276
EcoT38I GRGCYC 1 cut(s) 54
ErhI CCWWGG 2 cut(s) 158, 276
FaeI CATG 4 cut(s) 113, 272, 310, 358
FaiI YATR 6 cut(s) 48, 111, 270, 308, 356, 435
FalI AAGNNNNNCTT 2 cut(s) 324, 356
FaqI GGGAC 1 cut(s) 294
FatI CATG 4 cut(s) 109, 268, 306, 354
FbaI TGATCA 1 cut(s) 106
FblI GTMKAC 1 cut(s) 199
FokI GGATG 2 cut(s) 366, 377
FriOI GRGCYC 1 cut(s) 54
FspBI CTAG 4 cut(s) 81, 159, 212, 396
HapII CCGG 2 cut(s) 167, 231
Hin1II CATG 4 cut(s) 113, 272, 310, 358
HinfI GANTC 4 cut(s) 128, 134, 140, 265
HpaII CCGG 2 cut(s) 167, 231
HphI GGTGA 1 cut(s) 511
Hpy166II GTNNAC 4 cut(s) 127, 200, 223, 370
Hpy188I TCNGA 3 cut(s) 133, 139, 145
Hpy188III TCNNGA 5 cut(s) 101, 110, 231, 253, 307
Hpy8I GTNNAC 4 cut(s) 127, 200, 223, 370
HpyCH4III ACNGT 2 cut(s) 220, 227
HpyCH4IV ACGT 3 cut(s) 22, 421, 507
HpyCH4V TGCA 3 cut(s) 26, 63, 445
HpySE526I ACGT 3 cut(s) 22, 421, 507
Hsp92II CATG 4 cut(s) 113, 272, 310, 358
Kpn2I TCCGGA 1 cut(s) 230
Ksp22I TGATCA 1 cut(s) 106
Kzo9I GATC 2 cut(s) 106, 375
LmnI GCTCC 1 cut(s) 300
LpnPI CCDG 9 cut(s) 79, 180, 244, 266, 275, 346, 358, 400, 401
MaeI CTAG 4 cut(s) 81, 159, 212, 396
MaeII ACGT 3 cut(s) 22, 421, 507
MaeIII GTNAC 3 cut(s) 89, 271, 461
MalI GATC 2 cut(s) 108, 377
MboI GATC 2 cut(s) 106, 375
MboII GAAGA 3 cut(s) 97, 189, 493
MflI RGATCY 1 cut(s) 375
MhlI GDGCHC 1 cut(s) 54
MluCI AATT 3 cut(s) 42, 147, 182
MlyI GAGTC 2 cut(s) 122, 259
MmeI TCCRAC 1 cut(s) 328
MnlI CCTC 7 cut(s) 188, 213, 291, 303, 404, 442, 484
MroI TCCGGA 1 cut(s) 230
MseI TTAA 1 cut(s) 74
MspI CCGG 2 cut(s) 167, 231
NdeII GATC 2 cut(s) 106, 375
NlaIII CATG 4 cut(s) 113, 272, 310, 358
NlaIV GGNNCC 3 cut(s) 165, 282, 377
NmuCI GTSAC 3 cut(s) 89, 271, 461
PagI TCATGA 2 cut(s) 109, 306
PfeI GAWTC 2 cut(s) 134, 140
PflFI GACNNNGTC 1 cut(s) 356
PinAI ACCGGT 1 cut(s) 166
PleI GAGTC 2 cut(s) 122, 259
PpsI GAGTC 2 cut(s) 122, 259
Psp1406I AACGTT 1 cut(s) 507
PspN4I GGNNCC 3 cut(s) 165, 282, 377
PspPI GGNCC 1 cut(s) 281
PstNI CAGNNNCTG 1 cut(s) 143
PsuI RGATCY 1 cut(s) 375
PsyI GACNNNGTC 1 cut(s) 356
RsaI GTAC 1 cut(s) 14
RsaNI GTAC 1 cut(s) 13
SaqAI TTAA 1 cut(s) 74
Sau3AI GATC 2 cut(s) 106, 375
Sau96I GGNCC 1 cut(s) 281
SchI GAGTC 2 cut(s) 122, 259
SduI GDGCHC 1 cut(s) 54
SinI GGWCC 1 cut(s) 281
Sse9I AATT 3 cut(s) 42, 147, 182
SsiI CCGC 1 cut(s) 497
SspMI CTAG 4 cut(s) 81, 159, 212, 396
StyI CCWWGG 2 cut(s) 158, 276
TaaI ACNGT 2 cut(s) 220, 227
TaiI ACGT 3 cut(s) 25, 424, 510
TaqI TCGA 1 cut(s) 479
TasI AATT 3 cut(s) 42, 147, 182
TatI WGTACW 1 cut(s) 12
TfiI GAWTC 2 cut(s) 134, 140
Tru1I TTAA 1 cut(s) 74
Tru9I TTAA 1 cut(s) 74
TscAI CASTG 3 cut(s) 65, 253, 412
TseFI GTSAC 3 cut(s) 89, 271, 461
Tsp45I GTSAC 3 cut(s) 89, 271, 461
TspDTI ATGAA 2 cut(s) 295, 518
TspGWI ACGGA 1 cut(s) 453
TspRI CASTG 3 cut(s) 65, 253, 412
Tth111I GACNNNGTC 1 cut(s) 356
VpaK11BI GGWCC 1 cut(s) 281
XapI RAATTY 1 cut(s) 42
XmaJI CCTAGG 1 cut(s) 158
XmiI GTMKAC 1 cut(s) 199
XspI CTAG 4 cut(s) 81, 159, 212, 396
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.