RLG00000028372

B3 DNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0002871
Physical Location & Seq
Reverse (-)
21973847 .. 21975648
1802 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028372

Sequence Viewer

Length: 540 bp
ATGAAGACTCTGAATTTCCAGGCTTCTACAAGCGTGTTGATTACTCTGTTCTTCAAAAAGGGAAGCTTTAAGAAAAGTGACAGGCTTCATGATGTTACAATTGTTGACTCTGATTCTGATTGTGAACCGCAAAACACTGAAATAAGCTTGGAGTCTGAAATTGAGTGTGCTAGGGAATTGGGTCGTCGAACACATTTTTCAAGGAGAGGTAGAGCTCGTAACCTAGCCACCGTAAGCTGTTCCGGAAATCCTTGCTTCAGTGTCCAGATGTGCCAAACTCATGTGACCAAGGGACCATTGCTCACACCAAGGAGCTTCATGAGGAACATCTACAAGCCAAAGAAAGTCGAGCTTCAAGTTGGAAATATGTCCTGGGATGTGAACTGGATCCATCTTTACCAGAGATCTAGTAGGTTCAGTGGAGGTTGGCTACAGTTTGTGAAACAGAATGGGTTGCAAGAGGGAGATATCTGTGACTTTGAGCTGATTTCAAAGACATTTGAGGTTGCGGTGATGAAGGTTTCGATTAGGAAAGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

180

Amino Acids

20.49

Weight (kDa)

9.36

Isoelectric Point (pI)

43.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 88 - 172 8.3e-13 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 242
AciI CCGC 2 cut(s) 128, 509
AclWI GGATC 2 cut(s) 382, 395
AcsI RAATTY 1 cut(s) 13
AcuI CTGAAG 1 cut(s) 241
AgsI TTSAA 4 cut(s) 55, 201, 356, 492
AjnI CCWGG 2 cut(s) 18, 371
AluBI AGCT 7 cut(s) 66, 147, 215, 237, 315, 352, 484
AluI AGCT 7 cut(s) 66, 147, 215, 237, 315, 352, 484
Alw21I GWGCWC 1 cut(s) 217
AlwI GGATC 2 cut(s) 382, 395
Aor13HI TCCGGA 1 cut(s) 242
ApoI RAATTY 1 cut(s) 13
AspS9I GGNCC 1 cut(s) 293
AsuHPI GGTGA 1 cut(s) 523
AvaII GGWCC 1 cut(s) 293
BamHI GGATCC 1 cut(s) 387
BanII GRGCYC 1 cut(s) 217
BbsI GAAGAC 1 cut(s) 11
Bbv12I GWGCWC 1 cut(s) 217
BccI CCATC 1 cut(s) 399
BciT130I CCWGG 2 cut(s) 20, 373
BfaI CTAG 3 cut(s) 171, 224, 408
BfmI CTRYAG 1 cut(s) 431
BglII AGATCT 1 cut(s) 404
Bme1390I CCNGG 2 cut(s) 20, 373
Bme18I GGWCC 1 cut(s) 293
BmgT120I GGNCC 1 cut(s) 293
BmiI GGNNCC 2 cut(s) 294, 389
BmrFI CCNGG 2 cut(s) 20, 373
BpiI GAAGAC 1 cut(s) 11
BsaJI CCNNGG 3 cut(s) 288, 308, 372
BsaWI WCCGGW 1 cut(s) 242
Bse1I ACTGG 1 cut(s) 389
Bse3DI GCAATG 1 cut(s) 296
BseAI TCCGGA 1 cut(s) 242
BseBI CCWGG 2 cut(s) 20, 373
BseDI CCNNGG 3 cut(s) 288, 308, 372
BseGI GGATG 1 cut(s) 382
BseMI GCAATG 1 cut(s) 296
BseNI ACTGG 1 cut(s) 389
BsiHKAI GWGCWC 1 cut(s) 217
BsiSI CCGG 1 cut(s) 243
BslFI GGGAC 1 cut(s) 306
BsmFI GGGAC 1 cut(s) 306
Bsp1286I GDGCHC 1 cut(s) 217
Bsp13I TCCGGA 1 cut(s) 242
Bsp143I GATC 2 cut(s) 387, 404
BspACI CCGC 2 cut(s) 128, 509
BspEI TCCGGA 1 cut(s) 242
BspHI TCATGA 2 cut(s) 88, 318
BspLI GGNNCC 2 cut(s) 294, 389
BspPI GGATC 2 cut(s) 382, 395
BsrDI GCAATG 1 cut(s) 296
BsrI ACTGG 1 cut(s) 389
BssECI CCNNGG 3 cut(s) 288, 308, 372
BssMI GATC 2 cut(s) 387, 404
BssT1I CCWWGG 2 cut(s) 288, 308
Bst2UI CCWGG 2 cut(s) 20, 373
Bst4CI ACNGT 2 cut(s) 232, 435
BstF5I GGATG 1 cut(s) 382
BstKTI GATC 2 cut(s) 390, 407
BstMBI GATC 2 cut(s) 387, 404
BstNI CCWGG 2 cut(s) 20, 373
BstSCI CCNGG 2 cut(s) 18, 371
BstSFI CTRYAG 1 cut(s) 431
BstV2I GAAGAC 1 cut(s) 11
BstX2I RGATCY 2 cut(s) 387, 404
BstYI RGATCY 2 cut(s) 387, 404
BtsCI GGATG 1 cut(s) 382
BtsIMutI CAGTG 3 cut(s) 135, 265, 424
CciI TCATGA 2 cut(s) 88, 318
Cfr13I GGNCC 1 cut(s) 293
CviAII CATG 3 cut(s) 89, 281, 319
DpnI GATC 2 cut(s) 389, 406
DpnII GATC 2 cut(s) 387, 404
Ecl136II GAGCTC 1 cut(s) 215
Eco130I CCWWGG 2 cut(s) 288, 308
Eco24I GRGCYC 1 cut(s) 217
Eco32I GATATC 1 cut(s) 469
Eco47I GGWCC 1 cut(s) 293
Eco53kI GAGCTC 1 cut(s) 215
Eco57I CTGAAG 1 cut(s) 241
EcoICRI GAGCTC 1 cut(s) 215
EcoRII CCWGG 2 cut(s) 18, 371
EcoRV GATATC 1 cut(s) 469
EcoT14I CCWWGG 2 cut(s) 288, 308
EcoT38I GRGCYC 1 cut(s) 217
ErhI CCWWGG 2 cut(s) 288, 308
FaeI CATG 3 cut(s) 92, 284, 322
FaiI YATR 4 cut(s) 90, 282, 320, 368
FalI AAGNNNNNCTT 4 cut(s) 50, 82, 336, 368
FaqI GGGAC 1 cut(s) 306
FatI CATG 3 cut(s) 88, 280, 318
FokI GGATG 1 cut(s) 389
FriOI GRGCYC 1 cut(s) 217
FspBI CTAG 3 cut(s) 171, 224, 408
HapII CCGG 1 cut(s) 243
Hin1II CATG 3 cut(s) 92, 284, 322
HincII GTYRAC 1 cut(s) 106
HindII GTYRAC 1 cut(s) 106
HindIII AAGCTT 2 cut(s) 64, 145
HinfI GANTC 4 cut(s) 7, 107, 113, 152
HpaII CCGG 1 cut(s) 243
HphI GGTGA 1 cut(s) 523
Hpy166II GTNNAC 3 cut(s) 106, 125, 382
Hpy188I TCNGA 4 cut(s) 12, 112, 118, 157
Hpy188III TCNNGA 4 cut(s) 89, 243, 265, 319
Hpy8I GTNNAC 3 cut(s) 106, 125, 382
Hpy99I CGWCG 1 cut(s) 189
HpyAV CCTTC 1 cut(s) 511
HpyCH4III ACNGT 2 cut(s) 232, 435
HpyCH4V TGCA 1 cut(s) 457
Hsp92II CATG 3 cut(s) 92, 284, 322
Kpn2I TCCGGA 1 cut(s) 242
Kzo9I GATC 2 cut(s) 387, 404
LmnI GCTCC 1 cut(s) 312
LpnPI CCDG 9 cut(s) 5, 32, 67, 256, 278, 358, 370, 385, 413
MaeI CTAG 3 cut(s) 171, 224, 408
MaeIII GTNAC 5 cut(s) 77, 94, 218, 283, 473
MalI GATC 2 cut(s) 389, 406
MboI GATC 2 cut(s) 387, 404
MboII GAAGA 2 cut(s) 16, 43
MfeI CAATTG 1 cut(s) 99
MflI RGATCY 2 cut(s) 387, 404
MhlI GDGCHC 1 cut(s) 217
MluCI AATT 4 cut(s) 13, 99, 159, 176
MlyI GAGTC 2 cut(s) 101, 161
MmeI TCCRAC 1 cut(s) 340
MnlI CCTC 5 cut(s) 200, 315, 416, 454, 496
MroI TCCGGA 1 cut(s) 242
MseI TTAA 1 cut(s) 69
MspI CCGG 1 cut(s) 243
MspR9I CCNGG 2 cut(s) 20, 373
MunI CAATTG 1 cut(s) 99
MvaI CCWGG 2 cut(s) 20, 373
NdeII GATC 2 cut(s) 387, 404
NlaIII CATG 3 cut(s) 92, 284, 322
NlaIV GGNNCC 2 cut(s) 294, 389
NmuCI GTSAC 3 cut(s) 77, 283, 473
PagI TCATGA 2 cut(s) 88, 318
PfeI GAWTC 1 cut(s) 113
PleI GAGTC 2 cut(s) 101, 160
PpsI GAGTC 2 cut(s) 101, 160
Psp124BI GAGCTC 1 cut(s) 217
Psp6I CCWGG 2 cut(s) 18, 371
PspGI CCWGG 2 cut(s) 18, 371
PspN4I GGNNCC 2 cut(s) 294, 389
PspPI GGNCC 1 cut(s) 293
PsuI RGATCY 2 cut(s) 387, 404
SacI GAGCTC 1 cut(s) 217
SaqAI TTAA 1 cut(s) 69
Sau3AI GATC 2 cut(s) 387, 404
Sau96I GGNCC 1 cut(s) 293
SchI GAGTC 2 cut(s) 101, 161
ScrFI CCNGG 2 cut(s) 20, 373
SduI GDGCHC 1 cut(s) 217
SfcI CTRYAG 1 cut(s) 431
SinI GGWCC 1 cut(s) 293
Sse9I AATT 4 cut(s) 13, 99, 159, 176
SsiI CCGC 2 cut(s) 128, 509
SspMI CTAG 3 cut(s) 171, 224, 408
SstI GAGCTC 1 cut(s) 217
StyD4I CCNGG 2 cut(s) 18, 371
StyI CCWWGG 2 cut(s) 288, 308
TaaI ACNGT 2 cut(s) 232, 435
TaqI TCGA 3 cut(s) 187, 348, 524
TasI AATT 4 cut(s) 13, 99, 159, 176
TfiI GAWTC 1 cut(s) 113
Tru1I TTAA 1 cut(s) 69
Tru9I TTAA 1 cut(s) 69
TscAI CASTG 3 cut(s) 142, 265, 424
TseFI GTSAC 3 cut(s) 77, 283, 473
Tsp45I GTSAC 3 cut(s) 77, 283, 473
TspDTI ATGAA 4 cut(s) 17, 77, 307, 530
TspRI CASTG 3 cut(s) 142, 265, 424
VpaK11BI GGWCC 1 cut(s) 293
XapI RAATTY 1 cut(s) 13
XspI CTAG 3 cut(s) 171, 224, 408
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.