Rorug04G0316600

B3 DNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0002871
Physical Location & Seq
Forward (+)
49092062 .. 49093501
1440 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0316600.1

Sequence Viewer

Length: 714 bp
ATGTATCTTTACATTCGAATTGTGAAGGCGTTCCTAAAAGCATGGGAGGCTTTATGTGGATCAAGTGATGAAAGAACCCCTTCCCTGCTAATTCCAAAGGAAAGAACATTCTTGTTAAAACCTTTGGTTTTTAAAGGGCCTTGCAAGCCTGAAACCCTTAATTTCCAGCTCCAAGGGAGACTAGTTGCACCAGATTATAACAGTAAAGCATGGGAGTCAGACAAGGAAAAATGGGTTCGATCAGTTCTCAGATGTAGATGGTCTCATAATCATTGGATGAGCGCTCCTGATGAAAGTCCGAACACAGATGGGATTCAGATCATTTCATCAACTAATGTTAACATTGACAATTCTGCTATAGAAACTGGTGATGACTGCATTGCCATCAGTAGTGGTTCTTCTCAGATCCAAGTTAGTGGTGTTCGATGTGGACCTGGCCATGGTATAAGCATTGGAAGTCTTGGGAAAAATGGAGCTGACGCCACAGTGGAAGACGTGAACGTGAGAAATTGCACCTTAAGAGGAACACAAAATGGACTACGTATCAAGACATGGCCAGGTGGAAGAGGTTATGCTAGGAATATCACATATGAGGGAATCCGACTTATAGGGTCCAAGAACCCTATCATAATAAACCAGCACTATGTCGATAAAGGCCTGTCCGGACTCCGGACCAAAAGAAGCTGGCCGGAAAAGCTCAGTACAAGTGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

237

Amino Acids

26.26

Weight (kDa)

9.25

Isoelectric Point (pI)

35.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 93 - 224 2.5e-49 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 198
AccIII TCCGGA 2 cut(s) 662, 669
AclWI GGATC 2 cut(s) 67, 400
AcoI YGGCCR 3 cut(s) 436, 554, 686
AcyI GRCGYC 1 cut(s) 480
AfaI GTAC 1 cut(s) 703
AfeI AGCGCT 1 cut(s) 283
AfiI CCNNNNNNNGG 2 cut(s) 440, 669
AflII CTTAAG 1 cut(s) 517
AhlI ACTAGT 1 cut(s) 181
AjiI CACGTC 1 cut(s) 496
AjnI CCWGG 2 cut(s) 433, 556
AluBI AGCT 4 cut(s) 169, 476, 684, 697
AluI AGCT 4 cut(s) 169, 476, 684, 697
Alw26I GTCTC 2 cut(s) 172, 267
AlwI GGATC 2 cut(s) 67, 400
Aor13HI TCCGGA 2 cut(s) 662, 669
Aor51HI AGCGCT 1 cut(s) 283
AoxI GGCC 5 cut(s) 137, 436, 554, 655, 686
Asp700I GAANNNNTTC 2 cut(s) 29, 79
AspLEI GCGC 1 cut(s) 284
AspS9I GGNCC 4 cut(s) 137, 431, 612, 672
AsuHPI GGTGA 1 cut(s) 380
AsuII TTCGAA 1 cut(s) 16
AvaII GGWCC 3 cut(s) 431, 612, 672
BalI TGGCCA 2 cut(s) 438, 556
BarI GAAGNNNNNNTAC 2 cut(s) 382, 414
BbsI GAAGAC 1 cut(s) 498
BccI CCATC 3 cut(s) 252, 302, 392
BciT130I CCWGG 2 cut(s) 435, 558
BcoDI GTCTC 2 cut(s) 172, 267
BcuI ACTAGT 1 cut(s) 181
BfaI CTAG 2 cut(s) 182, 576
BfmI CTRYAG 1 cut(s) 357
BfoI RGCGCY 1 cut(s) 285
BfrI CTTAAG 1 cut(s) 517
Bme1390I CCNGG 2 cut(s) 435, 558
Bme18I GGWCC 3 cut(s) 431, 612, 672
BmgBI CACGTC 1 cut(s) 496
BmgT120I GGNCC 4 cut(s) 137, 431, 612, 672
BmiI GGNNCC 1 cut(s) 613
BmrFI CCNGG 2 cut(s) 435, 558
BpiI GAAGAC 1 cut(s) 498
Bpu14I TTCGAA 1 cut(s) 16
BsaAI YACGTR 1 cut(s) 542
BsaBI GATNNNNATC 1 cut(s) 317
BsaHI GRCGYC 1 cut(s) 480
BsaI GGTCTC 1 cut(s) 267
BsaJI CCNNGG 2 cut(s) 172, 439
BsaWI WCCGGW 2 cut(s) 662, 669
Bsc4I CCNNNNNNNGG 2 cut(s) 440, 669
Bse1I ACTGG 1 cut(s) 370
Bse3DI GCAATG 1 cut(s) 378
Bse8I GATNNNNATC 1 cut(s) 317
BseAI TCCGGA 2 cut(s) 662, 669
BseBI CCWGG 2 cut(s) 435, 558
BseDI CCNNGG 2 cut(s) 172, 439
BseGI GGATG 1 cut(s) 282
BseJI GATNNNNATC 1 cut(s) 317
BseLI CCNNNNNNNGG 2 cut(s) 440, 669
BseMI GCAATG 1 cut(s) 378
BseMII CTCAG 3 cut(s) 262, 416, 712
BseNI ACTGG 1 cut(s) 370
BshFI GGCC 5 cut(s) 139, 438, 556, 657, 688
BsiSI CCGG 3 cut(s) 663, 670, 689
BslI CCNNNNNNNGG 2 cut(s) 440, 669
BsmAI GTCTC 2 cut(s) 172, 267
BsnI GGCC 5 cut(s) 139, 438, 556, 657, 688
Bso31I GGTCTC 1 cut(s) 267
Bsp119I TTCGAA 1 cut(s) 16
Bsp13I TCCGGA 2 cut(s) 662, 669
Bsp143I GATC 4 cut(s) 59, 239, 318, 405
Bsp19I CCATGG 1 cut(s) 439
BspANI GGCC 5 cut(s) 139, 438, 556, 657, 688
BspCNI CTCAG 3 cut(s) 261, 415, 711
BspEI TCCGGA 2 cut(s) 662, 669
BspLI GGNNCC 1 cut(s) 613
BspPI GGATC 2 cut(s) 67, 400
BspT104I TTCGAA 1 cut(s) 16
BspTI CTTAAG 1 cut(s) 517
BspTNI GGTCTC 1 cut(s) 267
BsrDI GCAATG 1 cut(s) 378
BsrI ACTGG 1 cut(s) 370
BssECI CCNNGG 2 cut(s) 172, 439
BssMI GATC 4 cut(s) 59, 239, 318, 405
BssNI GRCGYC 1 cut(s) 480
BssT1I CCWWGG 2 cut(s) 172, 439
Bst2UI CCWGG 2 cut(s) 435, 558
Bst4CI ACNGT 2 cut(s) 203, 487
Bst6I CTCTTC 1 cut(s) 559
BstACI GRCGYC 1 cut(s) 480
BstAFI CTTAAG 1 cut(s) 517
BstBAI YACGTR 1 cut(s) 542
BstBI TTCGAA 1 cut(s) 16
BstC8I GCNNGC 2 cut(s) 146, 686
BstDEI CTNAG 3 cut(s) 248, 402, 698
BstDSI CCRYGG 1 cut(s) 439
BstF5I GGATG 1 cut(s) 282
BstH2I RGCGCY 1 cut(s) 285
BstHHI GCGC 1 cut(s) 284
BstKTI GATC 4 cut(s) 62, 242, 321, 408
BstMAI GTCTC 2 cut(s) 172, 267
BstMBI GATC 4 cut(s) 59, 239, 318, 405
BstMWI GCNNNNNNNGC 3 cut(s) 47, 145, 694
BstNI CCWGG 2 cut(s) 435, 558
BstSCI CCNGG 2 cut(s) 433, 556
BstSFI CTRYAG 1 cut(s) 357
BstSNI TACGTA 1 cut(s) 542
BstV2I GAAGAC 1 cut(s) 498
BstX2I RGATCY 1 cut(s) 405
BstXI CCANNNNNNTGG 1 cut(s) 416
BstYI RGATCY 1 cut(s) 405
BsuRI GGCC 5 cut(s) 139, 438, 556, 657, 688
BtgI CCRYGG 1 cut(s) 439
BtrI CACGTC 1 cut(s) 496
BtsCI GGATG 1 cut(s) 282
BtsIMutI CAGTG 1 cut(s) 492
Cac8I GCNNGC 2 cut(s) 146, 686
CfoI GCGC 1 cut(s) 284
Cfr13I GGNCC 4 cut(s) 137, 431, 612, 672
CseI GACGC 1 cut(s) 488
Csp6I GTAC 1 cut(s) 702
CviAII CATG 4 cut(s) 42, 210, 440, 552
CviQI GTAC 1 cut(s) 702
DdeI CTNAG 3 cut(s) 248, 402, 698
DpnI GATC 4 cut(s) 61, 241, 320, 407
DpnII GATC 4 cut(s) 59, 239, 318, 405
DraI TTTAAA 1 cut(s) 133
EaeI YGGCCR 3 cut(s) 436, 554, 686
Eam1104I CTCTTC 1 cut(s) 559
EarI CTCTTC 1 cut(s) 559
Eco105I TACGTA 1 cut(s) 542
Eco130I CCWWGG 2 cut(s) 172, 439
Eco147I AGGCCT 1 cut(s) 657
Eco31I GGTCTC 1 cut(s) 267
Eco47I GGWCC 3 cut(s) 431, 612, 672
Eco47III AGCGCT 1 cut(s) 283
EcoO109I RGGNCCY 1 cut(s) 137
EcoRII CCWGG 2 cut(s) 433, 556
EcoT14I CCWWGG 2 cut(s) 172, 439
ErhI CCWWGG 2 cut(s) 172, 439
FaeI CATG 4 cut(s) 45, 213, 443, 555
FalI AAGNNNNNCTT 2 cut(s) 64, 96
FatI CATG 4 cut(s) 41, 209, 439, 551
FauNDI CATATG 1 cut(s) 589
FokI GGATG 1 cut(s) 289
FspBI CTAG 2 cut(s) 182, 576
GlaI GCGC 1 cut(s) 283
HaeII RGCGCY 1 cut(s) 285
HaeIII GGCC 5 cut(s) 139, 438, 556, 657, 688
HapII CCGG 3 cut(s) 663, 670, 689
HgaI GACGC 1 cut(s) 488
HhaI GCGC 1 cut(s) 284
Hin1I GRCGYC 1 cut(s) 480
Hin1II CATG 4 cut(s) 45, 213, 443, 555
Hin6I GCGC 1 cut(s) 282
HinP1I GCGC 1 cut(s) 282
HincII GTYRAC 1 cut(s) 340
HindII GTYRAC 1 cut(s) 340
HinfI GANTC 4 cut(s) 215, 313, 597, 666
HpaI GTTAAC 1 cut(s) 340
HpaII CCGG 3 cut(s) 663, 670, 689
HphI GGTGA 1 cut(s) 380
Hpy166II GTNNAC 3 cut(s) 340, 431, 499
Hpy188I TCNGA 6 cut(s) 220, 251, 300, 318, 405, 602
Hpy188III TCNNGA 4 cut(s) 287, 547, 663, 670
Hpy8I GTNNAC 3 cut(s) 340, 431, 499
HpyAV CCTTC 2 cut(s) 19, 90
HpyCH4III ACNGT 2 cut(s) 203, 487
HpyCH4IV ACGT 3 cut(s) 495, 501, 541
HpyCH4V TGCA 4 cut(s) 144, 188, 378, 513
HpyF10VI GCNNNNNNNGC 3 cut(s) 47, 145, 694
HpyF3I CTNAG 3 cut(s) 248, 402, 698
HpySE526I ACGT 3 cut(s) 495, 501, 541
Hsp92I GRCGYC 1 cut(s) 480
Hsp92II CATG 4 cut(s) 45, 213, 443, 555
HspAI GCGC 1 cut(s) 282
Kpn2I TCCGGA 2 cut(s) 662, 669
KspAI GTTAAC 1 cut(s) 340
Kzo9I GATC 4 cut(s) 59, 239, 318, 405
LmnI GCTCC 3 cut(s) 174, 289, 473
MaeI CTAG 2 cut(s) 182, 576
MaeII ACGT 3 cut(s) 495, 501, 541
MalI GATC 4 cut(s) 61, 241, 320, 407
MboI GATC 4 cut(s) 59, 239, 318, 405
MboII GAAGA 3 cut(s) 390, 503, 576
MflI RGATCY 1 cut(s) 405
MlsI TGGCCA 2 cut(s) 438, 556
MluCI AATT 5 cut(s) 18, 90, 160, 349, 508
MluNI TGGCCA 2 cut(s) 438, 556
MlyI GAGTC 2 cut(s) 224, 660
MmeI TCCRAC 1 cut(s) 625
MnlI CCTC 4 cut(s) 40, 515, 560, 586
Mox20I TGGCCA 2 cut(s) 438, 556
MroI TCCGGA 2 cut(s) 662, 669
MroXI GAANNNNTTC 2 cut(s) 29, 79
MscI TGGCCA 2 cut(s) 438, 556
MseI TTAA 5 cut(s) 116, 132, 159, 339, 518
Msp20I TGGCCA 2 cut(s) 438, 556
MspCI CTTAAG 1 cut(s) 517
MspI CCGG 3 cut(s) 663, 670, 689
MspR9I CCNGG 2 cut(s) 435, 558
MvaI CCWGG 2 cut(s) 435, 558
MwoI GCNNNNNNNGC 3 cut(s) 47, 145, 694
NcoI CCATGG 1 cut(s) 439
NdeI CATATG 1 cut(s) 589
NdeII GATC 4 cut(s) 59, 239, 318, 405
NlaIII CATG 4 cut(s) 45, 213, 443, 555
NlaIV GGNNCC 1 cut(s) 613
NspV TTCGAA 1 cut(s) 16
PceI AGGCCT 1 cut(s) 657
PdmI GAANNNNTTC 2 cut(s) 29, 79
PfeI GAWTC 2 cut(s) 313, 597
PleI GAGTC 2 cut(s) 223, 660
PpsI GAGTC 2 cut(s) 223, 660
Ppu21I YACGTR 1 cut(s) 542
PsiI TTATAA 1 cut(s) 198
Psp6I CCWGG 2 cut(s) 433, 556
PspGI CCWGG 2 cut(s) 433, 556
PspN4I GGNNCC 1 cut(s) 613
PspPI GGNCC 4 cut(s) 137, 431, 612, 672
PsuI RGATCY 1 cut(s) 405
RsaI GTAC 1 cut(s) 703
RsaNI GTAC 1 cut(s) 702
SaqAI TTAA 5 cut(s) 116, 132, 159, 339, 518
Sau3AI GATC 4 cut(s) 59, 239, 318, 405
Sau96I GGNCC 4 cut(s) 137, 431, 612, 672
SchI GAGTC 2 cut(s) 224, 660
ScrFI CCNGG 2 cut(s) 435, 558
SfcI CTRYAG 1 cut(s) 357
SfuI TTCGAA 1 cut(s) 16
SinI GGWCC 3 cut(s) 431, 612, 672
SmlI CTYRAG 1 cut(s) 517
SmoI CTYRAG 1 cut(s) 517
SnaBI TACGTA 1 cut(s) 542
SpeI ACTAGT 1 cut(s) 181
Sse9I AATT 5 cut(s) 18, 90, 160, 349, 508
SseBI AGGCCT 1 cut(s) 657
SspMI CTAG 2 cut(s) 182, 576
StuI AGGCCT 1 cut(s) 657
StyD4I CCNGG 2 cut(s) 433, 556
StyI CCWWGG 2 cut(s) 172, 439
TaaI ACNGT 2 cut(s) 203, 487
TaiI ACGT 3 cut(s) 498, 504, 544
TaqI TCGA 4 cut(s) 16, 238, 424, 648
TasI AATT 5 cut(s) 18, 90, 160, 349, 508
TatI WGTACW 1 cut(s) 701
TfiI GAWTC 2 cut(s) 313, 597
Tru1I TTAA 5 cut(s) 116, 132, 159, 339, 518
Tru9I TTAA 5 cut(s) 116, 132, 159, 339, 518
TscAI CASTG 1 cut(s) 492
TspDTI ATGAA 3 cut(s) 84, 306, 315
TspRI CASTG 1 cut(s) 492
Vha464I CTTAAG 1 cut(s) 517
VpaK11BI GGWCC 3 cut(s) 431, 612, 672
XmnI GAANNNNTTC 2 cut(s) 29, 79
XspI CTAG 2 cut(s) 182, 576
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.