Rorug04G0316700

B3 DNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0002871
Physical Location & Seq
Forward (+)
49095121 .. 49096294
1174 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0316700.1

Sequence Viewer

Length: 903 bp
ATGGCAGCTGAGTTACAGCCGCTGGAGGCTACAATCCCCGCCGGCCCAACCGCCACCGCCACGTCGACACCGTCACCTCTTATAACCCCAACGGCGACGGCCGCAATAGCAACCACAACAGCAGCAGCAGCAGCAACAGCAACGGCTCTACACTCGGGCGAAAACCCGGGCCCCCAATTCCAACTGGGCCAGAAACGGCAGCGCCGGCCCAGCGTCCGGTTAGGCGAGATCGGCGACCAGCCCGCCGCCACCGTCTCAATCGACTCACACATGCGAAGGGCCAAGTACCCCTGGAAGCTCCCGAAGGACTCTAGTAAATCCGTCAAGGCCAGGTCGATTACGAACCTCGTCAACGGCCACGATTCGACTGAGATAGATGATCGGAACCAGAACGGCGACGGGGGATTCGAGTTCGGGAGCAGGAAGATCAAATCGAAGCGGGCGGCGACGACGAAGAGAGTCCGATCGAATTGGATTTCGAATTCGAAGATTGACGAGGGAGGGGATAGCCGGGAGGAGGGGGAGGGGTGGATGAGCGCAGATCCCGATAGGGACAGTCCGGTGAGGGATGAGAGTCCGGTGCAGTCGGCGGAGAACGAGGCGCACAATTGGGAGGAGCAGAGGAGGGTTGGGGGGAACAATTGGGGGAGGGGATCGGAGAGCAGGGAAAACGACGCCGTTGAGGCGGATAATATGCCGGAGTCGAATTGGAGCGGAGTGAGGTCGTGGCTAATAGAGCTGGGGCTGAGTCGGTATACGCCGGTGTTCGAGATACATGAGGTGGATGATGAGGTGTTGCCGATGCTGACATTGGAGGATCTTAAAGATATGGGGATAAACGCCGTCGGATCCAGGCGGAAAATGTACAGTGCTATCCAGAAGCTTCGCAAGGGGTTTTCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

300

Amino Acids

32.77

Weight (kDa)

5.85

Isoelectric Point (pI)

58.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAM_1 PF00536 239 - 296 2.2e-13 SAM domain (Sterile alpha motif)
SAM_2 PF07647 240 - 296 1.3e-13 SAM domain (Sterile alpha motif)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 83
AccBSI CCGCTC 1 cut(s) 714
AccI GTMKAC 2 cut(s) 65, 755
AclWI GGATC 5 cut(s) 536, 661, 825, 843, 856
AcoI YGGCCR 2 cut(s) 99, 355
AcsI RAATTY 1 cut(s) 481
AcyI GRCGYC 1 cut(s) 675
AfaI GTAC 2 cut(s) 287, 866
AfiI CCNNNNNNNGG 2 cut(s) 216, 517
AjiI CACGTC 1 cut(s) 63
AjnI CCWGG 3 cut(s) 290, 329, 851
AluBI AGCT 4 cut(s) 8, 298, 739, 883
AluI AGCT 4 cut(s) 8, 298, 739, 883
Alw26I GTCTC 1 cut(s) 259
AlwI GGATC 5 cut(s) 536, 661, 825, 843, 856
AlwNI CAGNNNCTG 1 cut(s) 22
Ama87I CYCGRG 2 cut(s) 154, 166
AoxI GGCC 8 cut(s) 43, 99, 169, 187, 206, 279, 327, 355
ApaI GGGCCC 1 cut(s) 173
ApeKI GCWGC 6 cut(s) 5, 122, 125, 128, 131, 199
ApoI RAATTY 1 cut(s) 481
ArsI GACNNNNNNTTYG 2 cut(s) 389, 421
AspLEI GCGC 3 cut(s) 204, 539, 604
AspS9I GGNCC 6 cut(s) 44, 169, 170, 187, 207, 279
AsuC2I CCSGG 3 cut(s) 167, 168, 512
AsuHPI GGTGA 2 cut(s) 66, 574
AsuII TTCGAA 2 cut(s) 479, 485
AvaI CYCGRG 2 cut(s) 154, 166
BaeGI GKGCMC 1 cut(s) 173
BamHI GGATCC 1 cut(s) 848
BanII GRGCYC 1 cut(s) 173
BbvI GCAGC 6 cut(s) 17, 134, 137, 140, 143, 211
BceAI ACGGC 8 cut(s) 108, 114, 159, 212, 370, 409, 662, 827
BciT130I CCWGG 3 cut(s) 292, 331, 853
BcnI CCSGG 3 cut(s) 167, 168, 512
BcoDI GTCTC 1 cut(s) 259
BfaI CTAG 1 cut(s) 312
BfoI RGCGCY 1 cut(s) 205
BglI GCCNNNNNGGC 1 cut(s) 683
Bme1390I CCNGG 6 cut(s) 167, 168, 292, 331, 512, 853
BmeT110I CYCGRG 2 cut(s) 154, 166
BmgBI CACGTC 1 cut(s) 63
BmgT120I GGNCC 6 cut(s) 44, 169, 170, 187, 207, 279
BmiI GGNNCC 4 cut(s) 171, 172, 386, 850
BmrFI CCNGG 6 cut(s) 167, 168, 292, 331, 512, 853
BmrI ACTGGG 1 cut(s) 194
BmsI GCATC 1 cut(s) 792
BmuI ACTGGG 1 cut(s) 194
BpmI CTGGAG 1 cut(s) 44
Bpu14I TTCGAA 2 cut(s) 479, 485
BpuMI CCSGG 3 cut(s) 167, 168, 512
BsaHI GRCGYC 1 cut(s) 675
BsaJI CCNNGG 2 cut(s) 166, 290
BsaWI WCCGGW 3 cut(s) 216, 559, 577
Bsc4I CCNNNNNNNGG 2 cut(s) 216, 517
Bse118I RCCGGY 3 cut(s) 41, 204, 760
Bse1I ACTGG 1 cut(s) 189
BseBI CCWGG 3 cut(s) 292, 331, 853
BseDI CCNNGG 2 cut(s) 166, 290
BseGI GGATG 3 cut(s) 537, 574, 790
BseLI CCNNNNNNNGG 2 cut(s) 216, 517
BseMII CTCAG 2 cut(s) 360, 737
BseNI ACTGG 1 cut(s) 189
BseRI GAGGAG 3 cut(s) 530, 629, 637
BseSI GKGCMC 1 cut(s) 173
BseX3I CGGCCG 1 cut(s) 99
BseXI GCAGC 6 cut(s) 17, 134, 137, 140, 143, 211
BseYI CCCAGC 2 cut(s) 209, 739
BsgI GTGCAG 1 cut(s) 602
Bsh1285I CGRYCG 2 cut(s) 102, 467
BshFI GGCC 8 cut(s) 45, 101, 171, 189, 208, 281, 329, 357
BsiEI CGRYCG 2 cut(s) 102, 467
BsiHKCI CYCGRG 2 cut(s) 154, 166
BsiSI CCGG 9 cut(s) 42, 167, 205, 217, 511, 560, 578, 698, 761
BslFI GGGAC 1 cut(s) 566
BslI CCNNNNNNNGG 2 cut(s) 216, 517
BsmAI GTCTC 1 cut(s) 259
BsmBI CGTCTC 1 cut(s) 259
BsmFI GGGAC 1 cut(s) 566
BsnI GGCC 8 cut(s) 45, 101, 171, 189, 208, 281, 329, 357
BsoBI CYCGRG 2 cut(s) 154, 166
Bsp119I TTCGAA 2 cut(s) 479, 485
Bsp120I GGGCCC 1 cut(s) 169
Bsp1286I GDGCHC 1 cut(s) 173
Bsp1407I TGTACA 1 cut(s) 864
Bsp143I GATC 8 cut(s) 228, 379, 426, 464, 541, 653, 817, 848
BspANI GGCC 8 cut(s) 45, 101, 171, 189, 208, 281, 329, 357
BspCNI CTCAG 2 cut(s) 361, 738
BspLI GGNNCC 4 cut(s) 171, 172, 386, 850
BspPI GGATC 5 cut(s) 536, 661, 825, 843, 856
BspT104I TTCGAA 2 cut(s) 479, 485
BsrBI CCGCTC 1 cut(s) 714
BsrFI RCCGGY 3 cut(s) 41, 204, 760
BsrGI TGTACA 1 cut(s) 864
BsrI ACTGG 1 cut(s) 189
BssAI RCCGGY 3 cut(s) 41, 204, 760
BssECI CCNNGG 2 cut(s) 166, 290
BssMI GATC 8 cut(s) 228, 379, 426, 464, 541, 653, 817, 848
BssNAI GTATAC 1 cut(s) 756
BssNI GRCGYC 1 cut(s) 675
Bst1107I GTATAC 1 cut(s) 756
Bst2UI CCWGG 3 cut(s) 292, 331, 853
Bst4CI ACNGT 4 cut(s) 72, 253, 557, 869
Bst6I CTCTTC 1 cut(s) 449
BstACI GRCGYC 1 cut(s) 675
BstAUI TGTACA 1 cut(s) 864
BstBI TTCGAA 2 cut(s) 479, 485
BstC8I GCNNGC 4 cut(s) 43, 206, 243, 441
BstDEI CTNAG 3 cut(s) 9, 369, 746
BstF5I GGATG 3 cut(s) 537, 574, 790
BstH2I RGCGCY 1 cut(s) 205
BstHHI GCGC 3 cut(s) 204, 539, 604
BstKTI GATC 8 cut(s) 231, 382, 429, 467, 544, 656, 820, 851
BstMAI GTCTC 1 cut(s) 259
BstMBI GATC 8 cut(s) 228, 379, 426, 464, 541, 653, 817, 848
BstMCI CGRYCG 2 cut(s) 102, 467
BstNI CCWGG 3 cut(s) 292, 331, 853
BstNSI RCATGY 1 cut(s) 274
BstSCI CCNGG 6 cut(s) 165, 166, 290, 329, 510, 851
BstSLI GKGCMC 1 cut(s) 173
BstV1I GCAGC 6 cut(s) 17, 134, 137, 140, 143, 211
BstX2I RGATCY 3 cut(s) 541, 817, 848
BstYI RGATCY 3 cut(s) 541, 817, 848
BstZ17I GTATAC 1 cut(s) 756
BstZI CGGCCG 1 cut(s) 99
BsuRI GGCC 8 cut(s) 45, 101, 171, 189, 208, 281, 329, 357
BtrI CACGTC 1 cut(s) 63
BtsCI GGATG 3 cut(s) 537, 574, 790
BtsIMutI CAGTG 1 cut(s) 874
Cac8I GCNNGC 4 cut(s) 43, 206, 243, 441
CaiI CAGNNNCTG 1 cut(s) 22
CfoI GCGC 3 cut(s) 204, 539, 604
Cfr10I RCCGGY 3 cut(s) 41, 204, 760
Cfr13I GGNCC 6 cut(s) 44, 169, 170, 187, 207, 279
Cfr9I CCCGGG 1 cut(s) 166
CseI GACGC 2 cut(s) 202, 683
Csp6I GTAC 2 cut(s) 286, 865
CviAII CATG 2 cut(s) 271, 776
CviQI GTAC 2 cut(s) 286, 865
DdeI CTNAG 3 cut(s) 9, 369, 746
DpnI GATC 8 cut(s) 230, 381, 428, 466, 543, 655, 819, 850
DpnII GATC 8 cut(s) 228, 379, 426, 464, 541, 653, 817, 848
EaeI YGGCCR 2 cut(s) 99, 355
EagI CGGCCG 1 cut(s) 99
Eam1104I CTCTTC 1 cut(s) 449
EarI CTCTTC 1 cut(s) 449
EciI GGCGGA 3 cut(s) 605, 701, 871
EclXI CGGCCG 1 cut(s) 99
Eco24I GRGCYC 1 cut(s) 173
Eco52I CGGCCG 1 cut(s) 99
Eco88I CYCGRG 2 cut(s) 154, 166
EcoO109I RGGNCCY 1 cut(s) 170
EcoRI GAATTC 1 cut(s) 481
EcoRII CCWGG 3 cut(s) 290, 329, 851
EcoT38I GRGCYC 1 cut(s) 173
Esp3I CGTCTC 1 cut(s) 259
FaeI CATG 2 cut(s) 274, 779
FaiI YATR 6 cut(s) 83, 272, 695, 756, 777, 830
FaqI GGGAC 1 cut(s) 566
FatI CATG 2 cut(s) 270, 775
FauI CCCGC 3 cut(s) 46, 250, 432
FblI GTMKAC 2 cut(s) 65, 755
FokI GGATG 3 cut(s) 544, 581, 797
FriOI GRGCYC 1 cut(s) 173
FspBI CTAG 1 cut(s) 312
GlaI GCGC 3 cut(s) 203, 538, 603
GsaI CCCAGC 2 cut(s) 213, 743
GsuI CTGGAG 1 cut(s) 44
HaeII RGCGCY 1 cut(s) 205
HaeIII GGCC 8 cut(s) 45, 101, 171, 189, 208, 281, 329, 357
HapII CCGG 9 cut(s) 42, 167, 205, 217, 511, 560, 578, 698, 761
HgaI GACGC 2 cut(s) 202, 683
HhaI GCGC 3 cut(s) 204, 539, 604
Hin1I GRCGYC 1 cut(s) 675
Hin1II CATG 2 cut(s) 274, 779
Hin6I GCGC 3 cut(s) 202, 537, 602
HinP1I GCGC 3 cut(s) 202, 537, 602
HincII GTYRAC 2 cut(s) 66, 352
HindII GTYRAC 2 cut(s) 66, 352
HindIII AAGCTT 1 cut(s) 881
HinfI GANTC 8 cut(s) 263, 308, 362, 405, 459, 574, 701, 748
HpaII CCGG 9 cut(s) 42, 167, 205, 217, 511, 560, 578, 698, 761
HphI GGTGA 2 cut(s) 66, 574
Hpy166II GTNNAC 3 cut(s) 66, 352, 756
Hpy188I TCNGA 4 cut(s) 384, 464, 658, 848
Hpy188III TCNNGA 6 cut(s) 301, 415, 545, 769, 877, 900
Hpy8I GTNNAC 3 cut(s) 66, 352, 756
Hpy99I CGWCG 7 cut(s) 67, 100, 401, 451, 454, 677, 848
HpyAV CCTTC 2 cut(s) 270, 298
HpyCH4III ACNGT 4 cut(s) 72, 253, 557, 869
HpyCH4IV ACGT 1 cut(s) 62
HpyCH4V TGCA 1 cut(s) 583
HpyF3I CTNAG 3 cut(s) 9, 369, 746
HpySE526I ACGT 1 cut(s) 62
Hsp92I GRCGYC 1 cut(s) 675
Hsp92II CATG 2 cut(s) 274, 779
HspAI GCGC 3 cut(s) 202, 537, 602
KroI GCCGGC 2 cut(s) 41, 204
KroNI GCCGGC 2 cut(s) 43, 206
Kzo9I GATC 8 cut(s) 228, 379, 426, 464, 541, 653, 817, 848
LmnI GCTCC 4 cut(s) 303, 417, 616, 711
Lsp1109I GCAGC 6 cut(s) 17, 134, 137, 140, 143, 211
LweI GCATC 1 cut(s) 792
MaeI CTAG 1 cut(s) 312
MaeII ACGT 1 cut(s) 62
MaeIII GTNAC 2 cut(s) 12, 72
MalI GATC 8 cut(s) 230, 381, 428, 466, 543, 655, 819, 850
MbiI CCGCTC 1 cut(s) 714
MboI GATC 8 cut(s) 228, 379, 426, 464, 541, 653, 817, 848
MboII GAAGA 3 cut(s) 436, 466, 499
MfeI CAATTG 2 cut(s) 607, 640
MflI RGATCY 3 cut(s) 541, 817, 848
MhlI GDGCHC 1 cut(s) 173
MluCI AATT 6 cut(s) 176, 469, 481, 607, 640, 706
MlyI GAGTC 6 cut(s) 257, 302, 468, 583, 710, 757
MmeI TCCRAC 2 cut(s) 205, 826
MroNI GCCGGC 2 cut(s) 41, 204
MseI TTAA 1 cut(s) 822
MspA1I CMGCKG 2 cut(s) 8, 22
MspI CCGG 9 cut(s) 42, 167, 205, 217, 511, 560, 578, 698, 761
MspR9I CCNGG 6 cut(s) 167, 168, 292, 331, 512, 853
MunI CAATTG 2 cut(s) 607, 640
MvaI CCWGG 3 cut(s) 292, 331, 853
NaeI GCCGGC 2 cut(s) 43, 206
NciI CCSGG 3 cut(s) 167, 168, 512
NdeII GATC 8 cut(s) 228, 379, 426, 464, 541, 653, 817, 848
NgoMIV GCCGGC 2 cut(s) 41, 204
NlaIII CATG 2 cut(s) 274, 779
NlaIV GGNNCC 4 cut(s) 171, 172, 386, 850
NmuCI GTSAC 1 cut(s) 72
NspI RCATGY 1 cut(s) 274
NspV TTCGAA 2 cut(s) 479, 485
PcsI WCGNNNNNNNCGW 2 cut(s) 68, 405
PdiI GCCGGC 2 cut(s) 43, 206
PfeI GAWTC 2 cut(s) 362, 405
PflFI GACNNNGTC 1 cut(s) 70
Ple19I CGATCG 1 cut(s) 467
PleI GAGTC 6 cut(s) 257, 302, 467, 582, 709, 756
PpsI GAGTC 6 cut(s) 257, 302, 467, 582, 709, 756
PsiI TTATAA 1 cut(s) 83
Psp6I CCWGG 3 cut(s) 290, 329, 851
PspFI CCCAGC 2 cut(s) 209, 739
PspGI CCWGG 3 cut(s) 290, 329, 851
PspN4I GGNNCC 4 cut(s) 171, 172, 386, 850
PspOMI GGGCCC 1 cut(s) 169
PspPI GGNCC 6 cut(s) 44, 169, 170, 187, 207, 279
PstNI CAGNNNCTG 1 cut(s) 22
PsuI RGATCY 3 cut(s) 541, 817, 848
PsyI GACNNNGTC 1 cut(s) 70
PvuI CGATCG 1 cut(s) 467
PvuII CAGCTG 1 cut(s) 8
RsaI GTAC 2 cut(s) 287, 866
RsaNI GTAC 2 cut(s) 286, 865
SalI GTCGAC 1 cut(s) 64
SaqAI TTAA 1 cut(s) 822
Sau3AI GATC 8 cut(s) 228, 379, 426, 464, 541, 653, 817, 848
Sau96I GGNCC 6 cut(s) 44, 169, 170, 187, 207, 279
SchI GAGTC 6 cut(s) 257, 302, 468, 583, 710, 757
ScrFI CCNGG 6 cut(s) 167, 168, 292, 331, 512, 853
SduI GDGCHC 1 cut(s) 173
SfaNI GCATC 1 cut(s) 792
SfuI TTCGAA 2 cut(s) 479, 485
SgrAI CRCCGGYG 1 cut(s) 760
SmaI CCCGGG 1 cut(s) 168
Sse9I AATT 6 cut(s) 176, 469, 481, 607, 640, 706
SspMI CTAG 1 cut(s) 312
StyD4I CCNGG 6 cut(s) 165, 166, 290, 329, 510, 851
TaaI ACNGT 4 cut(s) 72, 253, 557, 869
TaiI ACGT 1 cut(s) 65
TasI AATT 6 cut(s) 176, 469, 481, 607, 640, 706
TatI WGTACW 1 cut(s) 864
TauI GCSGC 4 cut(s) 22, 104, 248, 446
TfiI GAWTC 2 cut(s) 362, 405
Tru1I TTAA 1 cut(s) 822
Tru9I TTAA 1 cut(s) 822
TscAI CASTG 1 cut(s) 874
TseFI GTSAC 1 cut(s) 72
TseI GCWGC 6 cut(s) 5, 122, 125, 128, 131, 199
Tsp45I GTSAC 1 cut(s) 72
TspGWI ACGGA 1 cut(s) 310
TspMI CCCGGG 1 cut(s) 166
TspRI CASTG 1 cut(s) 874
Tth111I GACNNNGTC 1 cut(s) 70
XapI RAATTY 1 cut(s) 481
XceI RCATGY 1 cut(s) 274
XmaI CCCGGG 1 cut(s) 166
XmiI GTMKAC 2 cut(s) 65, 755
XspI CTAG 1 cut(s) 312
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.