Rorug01G0223100

B3 DNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0002871
Physical Location & Seq
Reverse (-)
32071181 .. 32071810
630 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0223100.1

Sequence Viewer

Length: 630 bp
ATGGAACCACAAACCCGGAAACCAACCGAGGATGGAGGAAGCAACAAAGCAGCCGGGAGTAGTGCTAACATGCTTTATAAGCGGAGTAGGTGGACTCCCACTACAGATCAGATAAGAATCCTCAAGGAGCTTTTCTACAATAAGGGAGTTAGGTCCCCAACTGTAGAGCAGGTTGAGAGGATCTATCTCCAGCTGAAATGGTACGGCAAGATCGAGTTCAAGAACGTCTATTTTTGGTTCATGAACCAAAGGGCTCGGGAGAAGCAGAAGAAGAAGTCCACTTCGGATGTTCATGTGCCCATGCAAAGATCAGGGCTTGTTGGTGATGATAATGTTGCCAATTCATCTGCACCTGCTTCTGCTTCTTCTGCTGGTGTGATGATTGCTGTTAACGGGCAGATGGGGAACTATGGTGGTTATGGATCTATGAACATGGAGATTGAAACCCTTCCTCTGTTCCCCATGCATGGTGAGGACATCTTTGGCAACATGAAGACTACTTCCGATGGAGGTGGCGGTCACGGTGGTGGCTCTCACATTTCCCTTGAGCTCAGCCTTTCCTGCGAGGGCAAAGCTCCAGAAGGCCACGGGGGAGGCCATCTACGCTTCACTCCAAGCTCAGATGCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

209

Amino Acids

22.78

Weight (kDa)

8.91

Isoelectric Point (pI)

53.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Homeodomain PF00046 27 - 89 5.8e-10 Homeodomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 78
AarI CACCTGC 1 cut(s) 361
Acc36I ACCTGC 2 cut(s) 160, 361
AciI CCGC 2 cut(s) 82, 516
AclWI GGATC 2 cut(s) 188, 430
AfaI GTAC 1 cut(s) 203
AfiI CCNNNNNNNGG 1 cut(s) 467
AgsI TTSAA 2 cut(s) 220, 443
AleI CACNNNNGTG 1 cut(s) 525
AluBI AGCT 5 cut(s) 130, 193, 550, 575, 618
AluI AGCT 5 cut(s) 130, 193, 550, 575, 618
Alw21I GWGCWC 1 cut(s) 552
AlwI GGATC 2 cut(s) 188, 430
Ama87I CYCGRG 1 cut(s) 255
AoxI GGCC 2 cut(s) 583, 595
ApeKI GCWGC 1 cut(s) 50
Asp700I GAANNNNTTC 1 cut(s) 447
AspS9I GGNCC 1 cut(s) 153
AsuC2I CCSGG 2 cut(s) 16, 55
AsuHPI GGTGA 2 cut(s) 335, 482
AvaI CYCGRG 1 cut(s) 255
AvaII GGWCC 1 cut(s) 153
BaeGI GKGCMC 1 cut(s) 300
BanII GRGCYC 2 cut(s) 256, 552
BbsI GAAGAC 1 cut(s) 500
Bbv12I GWGCWC 1 cut(s) 552
BbvI GCAGC 1 cut(s) 62
BccI CCATC 4 cut(s) 26, 394, 500, 606
BceAI ACGGC 1 cut(s) 220
BcnI CCSGG 2 cut(s) 16, 55
BfmI CTRYAG 2 cut(s) 102, 162
BfuAI ACCTGC 2 cut(s) 160, 361
BisI GCNGC 1 cut(s) 51
BlpI GCTNAGC 1 cut(s) 551
BlsI GCNGC 1 cut(s) 52
Bme1390I CCNGG 2 cut(s) 16, 55
Bme18I GGWCC 1 cut(s) 153
BmeT110I CYCGRG 1 cut(s) 255
BmgT120I GGNCC 1 cut(s) 153
BmiI GGNNCC 2 cut(s) 6, 155
BmrFI CCNGG 2 cut(s) 16, 55
BmsI GCATC 1 cut(s) 613
BpiI GAAGAC 1 cut(s) 500
BpmI CTGGAG 2 cut(s) 173, 561
Bpu1102I GCTNAGC 1 cut(s) 551
BpuEI CTTGAG 2 cut(s) 107, 566
BpuMI CCSGG 2 cut(s) 16, 55
BsaBI GATNNNNATC 1 cut(s) 116
BsaJI CCNNGG 2 cut(s) 27, 586
Bsc4I CCNNNNNNNGG 1 cut(s) 467
Bse8I GATNNNNATC 1 cut(s) 116
BseDI CCNNGG 2 cut(s) 27, 586
BseGI GGATG 2 cut(s) 37, 292
BseJI GATNNNNATC 1 cut(s) 116
BseLI CCNNNNNNNGG 1 cut(s) 467
BseMII CTCAG 1 cut(s) 565
BseSI GKGCMC 1 cut(s) 300
BseXI GCAGC 1 cut(s) 62
BsgI GTGCAG 1 cut(s) 333
BshFI GGCC 2 cut(s) 585, 597
BsiHKAI GWGCWC 1 cut(s) 552
BsiHKCI CYCGRG 1 cut(s) 255
BsiSI CCGG 2 cut(s) 16, 54
BslFI GGGAC 1 cut(s) 139
BslI CCNNNNNNNGG 1 cut(s) 467
BsmFI GGGAC 1 cut(s) 139
BsnI GGCC 2 cut(s) 585, 597
BsoBI CYCGRG 1 cut(s) 255
Bsp1286I GDGCHC 3 cut(s) 256, 300, 552
Bsp143I GATC 5 cut(s) 106, 180, 210, 308, 422
Bsp1720I GCTNAGC 1 cut(s) 551
BspACI CCGC 2 cut(s) 82, 516
BspANI GGCC 2 cut(s) 585, 597
BspCNI CTCAG 1 cut(s) 564
BspHI TCATGA 1 cut(s) 240
BspLI GGNNCC 2 cut(s) 6, 155
BspMI ACCTGC 2 cut(s) 160, 361
BspPI GGATC 2 cut(s) 188, 430
BssECI CCNNGG 2 cut(s) 27, 586
BssMI GATC 5 cut(s) 106, 180, 210, 308, 422
Bst4CI ACNGT 2 cut(s) 163, 524
BstDEI CTNAG 2 cut(s) 551, 619
BstDSI CCRYGG 1 cut(s) 586
BstF5I GGATG 2 cut(s) 37, 292
BstKTI GATC 5 cut(s) 109, 183, 213, 311, 425
BstMBI GATC 5 cut(s) 106, 180, 210, 308, 422
BstMWI GCNNNNNNNGC 4 cut(s) 79, 368, 561, 603
BstNSI RCATGY 1 cut(s) 73
BstSCI CCNGG 2 cut(s) 14, 53
BstSFI CTRYAG 2 cut(s) 102, 162
BstSLI GKGCMC 1 cut(s) 300
BstV1I GCAGC 1 cut(s) 62
BstV2I GAAGAC 1 cut(s) 500
BstX2I RGATCY 2 cut(s) 180, 422
BstYI RGATCY 2 cut(s) 180, 422
BsuRI GGCC 2 cut(s) 585, 597
BtgI CCRYGG 1 cut(s) 586
BtsCI GGATG 2 cut(s) 37, 292
BveI ACCTGC 2 cut(s) 160, 361
CciI TCATGA 1 cut(s) 240
Cfr13I GGNCC 1 cut(s) 153
Csp6I GTAC 1 cut(s) 202
CviAII CATG 8 cut(s) 70, 241, 293, 301, 433, 463, 467, 490
CviQI GTAC 1 cut(s) 202
DdeI CTNAG 2 cut(s) 551, 619
DpnI GATC 5 cut(s) 108, 182, 212, 310, 424
DpnII GATC 5 cut(s) 106, 180, 210, 308, 422
Ecl136II GAGCTC 1 cut(s) 550
Eco24I GRGCYC 2 cut(s) 256, 552
Eco47I GGWCC 1 cut(s) 153
Eco53kI GAGCTC 1 cut(s) 550
Eco88I CYCGRG 1 cut(s) 255
EcoICRI GAGCTC 1 cut(s) 550
EcoO109I RGGNCCY 1 cut(s) 153
EcoT22I ATGCAT 1 cut(s) 468
EcoT38I GRGCYC 2 cut(s) 256, 552
FaeI CATG 8 cut(s) 73, 244, 296, 304, 436, 466, 470, 493
FaqI GGGAC 1 cut(s) 139
FatI CATG 8 cut(s) 69, 240, 292, 300, 432, 462, 466, 489
Fnu4HI GCNGC 1 cut(s) 51
FokI GGATG 2 cut(s) 44, 299
FriOI GRGCYC 2 cut(s) 256, 552
Fsp4HI GCNGC 1 cut(s) 51
GluI GCNGC 1 cut(s) 51
GsuI CTGGAG 2 cut(s) 173, 561
HaeIII GGCC 2 cut(s) 585, 597
HapII CCGG 2 cut(s) 16, 54
Hin1II CATG 8 cut(s) 73, 244, 296, 304, 436, 466, 470, 493
HincII GTYRAC 1 cut(s) 391
HindII GTYRAC 1 cut(s) 391
HinfI GANTC 2 cut(s) 94, 117
HpaI GTTAAC 1 cut(s) 391
HpaII CCGG 2 cut(s) 16, 54
HphI GGTGA 2 cut(s) 335, 482
Hpy166II GTNNAC 3 cut(s) 93, 279, 391
Hpy188I TCNGA 4 cut(s) 111, 286, 505, 622
Hpy188III TCNNGA 4 cut(s) 220, 241, 257, 578
Hpy8I GTNNAC 3 cut(s) 93, 279, 391
HpyAV CCTTC 2 cut(s) 458, 575
HpyCH4III ACNGT 2 cut(s) 163, 524
HpyCH4IV ACGT 1 cut(s) 225
HpyCH4V TGCA 3 cut(s) 304, 350, 466
HpyF10VI GCNNNNNNNGC 4 cut(s) 79, 368, 561, 603
HpyF3I CTNAG 2 cut(s) 551, 619
HpySE526I ACGT 1 cut(s) 225
Hsp92II CATG 8 cut(s) 73, 244, 296, 304, 436, 466, 470, 493
KspAI GTTAAC 1 cut(s) 391
Kzo9I GATC 5 cut(s) 106, 180, 210, 308, 422
LmnI GCTCC 2 cut(s) 127, 580
LpnPI CCDG 9 cut(s) 29, 67, 155, 203, 297, 357, 366, 574, 591
Lsp1109I GCAGC 1 cut(s) 62
LweI GCATC 1 cut(s) 613
MaeII ACGT 1 cut(s) 225
MaeIII GTNAC 1 cut(s) 518
MalI GATC 5 cut(s) 108, 182, 212, 310, 424
MboI GATC 5 cut(s) 106, 180, 210, 308, 422
MboII GAAGA 4 cut(s) 280, 283, 357, 505
MflI RGATCY 2 cut(s) 180, 422
MhlI GDGCHC 3 cut(s) 256, 300, 552
MluCI AATT 1 cut(s) 340
MlyI GAGTC 1 cut(s) 88
MnlI CCTC 9 cut(s) 22, 29, 131, 171, 462, 466, 503, 559, 587
Mph1103I ATGCAT 1 cut(s) 468
MroXI GAANNNNTTC 1 cut(s) 447
MseI TTAA 1 cut(s) 390
MslI CAYNNNNRTG 1 cut(s) 525
MspA1I CMGCKG 1 cut(s) 193
MspI CCGG 2 cut(s) 16, 54
MspR9I CCNGG 2 cut(s) 16, 55
MwoI GCNNNNNNNGC 4 cut(s) 79, 368, 561, 603
NciI CCSGG 2 cut(s) 16, 55
NdeII GATC 5 cut(s) 106, 180, 210, 308, 422
NlaIII CATG 8 cut(s) 73, 244, 296, 304, 436, 466, 470, 493
NlaIV GGNNCC 2 cut(s) 6, 155
NmuCI GTSAC 1 cut(s) 518
NsiI ATGCAT 1 cut(s) 468
NspI RCATGY 1 cut(s) 73
OliI CACNNNNGTG 1 cut(s) 525
PagI TCATGA 1 cut(s) 240
PaqCI CACCTGC 1 cut(s) 361
PcsI WCGNNNNNNNCGW 1 cut(s) 210
PdmI GAANNNNTTC 1 cut(s) 447
PfeI GAWTC 1 cut(s) 117
PkrI GCNGC 1 cut(s) 52
PleI GAGTC 1 cut(s) 88
PpsI GAGTC 1 cut(s) 88
PpuMI RGGWCCY 1 cut(s) 153
PsiI TTATAA 1 cut(s) 78
Psp124BI GAGCTC 1 cut(s) 552
Psp5II RGGWCCY 1 cut(s) 153
PspN4I GGNNCC 2 cut(s) 6, 155
PspPI GGNCC 1 cut(s) 153
PspPPI RGGWCCY 1 cut(s) 153
PsuI RGATCY 2 cut(s) 180, 422
PvuII CAGCTG 1 cut(s) 193
RsaI GTAC 1 cut(s) 203
RsaNI GTAC 1 cut(s) 202
RseI CAYNNNNRTG 1 cut(s) 525
SacI GAGCTC 1 cut(s) 552
SaqAI TTAA 1 cut(s) 390
SatI GCNGC 1 cut(s) 51
Sau3AI GATC 5 cut(s) 106, 180, 210, 308, 422
Sau96I GGNCC 1 cut(s) 153
SchI GAGTC 1 cut(s) 88
ScrFI CCNGG 2 cut(s) 16, 55
SduI GDGCHC 3 cut(s) 256, 300, 552
SfaNI GCATC 1 cut(s) 613
SfcI CTRYAG 2 cut(s) 102, 162
SinI GGWCC 1 cut(s) 153
SmiMI CAYNNNNRTG 1 cut(s) 525
SmlI CTYRAG 2 cut(s) 122, 545
SmoI CTYRAG 2 cut(s) 122, 545
Sse9I AATT 1 cut(s) 340
SsiI CCGC 2 cut(s) 82, 516
SstI GAGCTC 1 cut(s) 552
StyD4I CCNGG 2 cut(s) 14, 53
TaaI ACNGT 2 cut(s) 163, 524
TaiI ACGT 1 cut(s) 228
TaqI TCGA 1 cut(s) 213
TasI AATT 1 cut(s) 340
TfiI GAWTC 1 cut(s) 117
Tru1I TTAA 1 cut(s) 390
Tru9I TTAA 1 cut(s) 390
TseFI GTSAC 1 cut(s) 518
TseI GCWGC 1 cut(s) 50
Tsp45I GTSAC 1 cut(s) 518
TspDTI ATGAA 6 cut(s) 229, 257, 281, 333, 443, 506
VpaK11BI GGWCC 1 cut(s) 153
XceI RCATGY 1 cut(s) 73
XmnI GAANNNNTTC 1 cut(s) 447
Zsp2I ATGCAT 1 cut(s) 468
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.