Rh4BG379000

B3 DNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0002871
Physical Location & Seq
Forward (+)
53366924 .. 53370011
3088 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG379000.1

Sequence Viewer

Length: 657 bp
ATGGGTTCTTGCAGCAGAAGAAGAGAGGATGAAGACTCTGAATTTCCAGGCTTCTACAAGTGTGTTGATTATTCTGTTCTTCTAAAGGGGAAGCTTAAAATCCCCCGAGATTTTTGCGAGAACTTTGAAGGAAAGATGCCTCAGCAGTGCCGTCTTCAGACTCTTTCTGGAACTTGGCATGTTGATGTGAAAAATATTAGCGGGGAGTTCTTCTTGAAAAGGGGCTGGAAGGAATTTGTTGTGGGAAATCGTTTGAAGCTAGGTGAATGTGTAGTCTTTGATTACCCTGGACATGCAAAGTTCTATGTTGAGATCTATGGACACAATCACTGCAGCAAGAGTTCAAAGGCAATGAGAAGTGAGAGGCTTCTTGATGATGACTCTGATGATCATAATGTTGTTTTTGTTGACTCGGAATCTGATTCTGAACCTCAAAACAGTGAAATCAGCTTGGAGTCTGAAACTGAGTGTCCTAGGAAATCAGGTTATGGAAAAAAATTGTCAAGGAGAGGTAGAGCTGGTAACTCAGCCACCATAAGACGTTTTGGTAATCCTTGCTTCAGTGTTAAGATGTGCCAAACTCATGTGACCAAGGGACCACTGGTATGGAAACATGAACCCAATCTGTTGATGCATATATCAAATTTGTTTTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

218

Amino Acids

25.05

Weight (kDa)

8.33

Isoelectric Point (pI)

57.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 25 - 107 5.3e-14 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 201
AcsI RAATTY 3 cut(s) 41, 233, 643
AcuI CTGAAG 2 cut(s) 140, 544
AgsI TTSAA 4 cut(s) 128, 217, 256, 345
AjnI CCWGG 2 cut(s) 46, 286
AluBI AGCT 4 cut(s) 94, 259, 450, 518
AluI AGCT 4 cut(s) 94, 259, 450, 518
Ama87I CYCGRG 1 cut(s) 105
ApeKI GCWGC 2 cut(s) 12, 333
ApoI RAATTY 3 cut(s) 41, 233, 643
AspA2I CCTAGG 1 cut(s) 473
AspS9I GGNCC 1 cut(s) 596
AsuHPI GGTGA 1 cut(s) 275
AvaI CYCGRG 1 cut(s) 105
AvaII GGWCC 1 cut(s) 596
AvrII CCTAGG 1 cut(s) 473
BbsI GAAGAC 2 cut(s) 39, 146
BbvCI CCTCAGC 1 cut(s) 141
BbvI GCAGC 2 cut(s) 24, 345
BceAI ACGGC 1 cut(s) 135
BcgI CGANNNNNNTGC 2 cut(s) 96, 130
BciT130I CCWGG 2 cut(s) 48, 288
BclI TGATCA 1 cut(s) 388
BfaI CTAG 2 cut(s) 260, 474
BfmI CTRYAG 1 cut(s) 331
BglII AGATCT 1 cut(s) 312
BisI GCNGC 2 cut(s) 13, 334
BlnI CCTAGG 1 cut(s) 473
BlsI GCNGC 2 cut(s) 14, 335
Bme1390I CCNGG 2 cut(s) 48, 288
Bme18I GGWCC 1 cut(s) 596
BmeT110I CYCGRG 1 cut(s) 105
BmgT120I GGNCC 1 cut(s) 596
BmiI GGNNCC 1 cut(s) 597
BmrFI CCNGG 2 cut(s) 48, 288
BmsI GCATC 2 cut(s) 126, 621
BpiI GAAGAC 2 cut(s) 39, 146
Bpu10I CCTNAGC 1 cut(s) 141
BsaJI CCNNGG 3 cut(s) 286, 473, 591
Bse1I ACTGG 1 cut(s) 606
Bse3DI GCAATG 1 cut(s) 357
BseBI CCWGG 2 cut(s) 48, 288
BseDI CCNNGG 3 cut(s) 286, 473, 591
BseGI GGATG 1 cut(s) 34
BseMI GCAATG 1 cut(s) 357
BseMII CTCAG 3 cut(s) 155, 456, 540
BseNI ACTGG 1 cut(s) 606
BseXI GCAGC 2 cut(s) 24, 345
BsiHKCI CYCGRG 1 cut(s) 105
BslFI GGGAC 1 cut(s) 609
BsmFI GGGAC 1 cut(s) 609
BsoBI CYCGRG 1 cut(s) 105
Bsp143I GATC 2 cut(s) 312, 388
BspACI CCGC 1 cut(s) 201
BspCNI CTCAG 3 cut(s) 154, 457, 539
BspLI GGNNCC 1 cut(s) 597
BspMAI CTGCAG 1 cut(s) 335
BsrDI GCAATG 1 cut(s) 357
BsrI ACTGG 1 cut(s) 606
BssECI CCNNGG 3 cut(s) 286, 473, 591
BssMI GATC 2 cut(s) 312, 388
BssT1I CCWWGG 2 cut(s) 473, 591
Bst2UI CCWGG 2 cut(s) 48, 288
Bst4CI ACNGT 1 cut(s) 440
Bst6I CTCTTC 1 cut(s) 16
BstDEI CTNAG 3 cut(s) 141, 465, 526
BstF5I GGATG 1 cut(s) 34
BstKTI GATC 2 cut(s) 315, 391
BstMBI GATC 2 cut(s) 312, 388
BstNI CCWGG 2 cut(s) 48, 288
BstNSI RCATGY 2 cut(s) 182, 296
BstSCI CCNGG 2 cut(s) 46, 286
BstSFI CTRYAG 1 cut(s) 331
BstV1I GCAGC 2 cut(s) 24, 345
BstV2I GAAGAC 2 cut(s) 39, 146
BstX2I RGATCY 1 cut(s) 312
BstXI CCANNNNNNTGG 1 cut(s) 606
BstYI RGATCY 1 cut(s) 312
BtsCI GGATG 1 cut(s) 34
BtsI GCAGTG 2 cut(s) 152, 328
BtsIMutI CAGTG 5 cut(s) 152, 328, 445, 568, 599
Cfr13I GGNCC 1 cut(s) 596
CviAII CATG 4 cut(s) 179, 293, 584, 614
CviJI RGCY 8 cut(s) 51, 94, 225, 259, 367, 450, 518, 530
CviKI_1 RGCY 8 cut(s) 51, 94, 225, 259, 367, 450, 518, 530
DdeI CTNAG 3 cut(s) 141, 465, 526
DpnI GATC 2 cut(s) 314, 390
DpnII GATC 2 cut(s) 312, 388
Eam1104I CTCTTC 1 cut(s) 16
EarI CTCTTC 1 cut(s) 16
Eco130I CCWWGG 2 cut(s) 473, 591
Eco47I GGWCC 1 cut(s) 596
Eco57I CTGAAG 2 cut(s) 140, 544
Eco88I CYCGRG 1 cut(s) 105
EcoRII CCWGG 2 cut(s) 46, 286
EcoT14I CCWWGG 2 cut(s) 473, 591
EcoT22I ATGCAT 1 cut(s) 636
ErhI CCWWGG 2 cut(s) 473, 591
FaeI CATG 4 cut(s) 182, 296, 587, 617
FaqI GGGAC 1 cut(s) 609
FatI CATG 4 cut(s) 178, 292, 583, 613
FauI CCCGC 1 cut(s) 194
FbaI TGATCA 1 cut(s) 388
Fnu4HI GCNGC 2 cut(s) 13, 334
FokI GGATG 1 cut(s) 41
Fsp4HI GCNGC 2 cut(s) 13, 334
FspBI CTAG 2 cut(s) 260, 474
GluI GCNGC 2 cut(s) 13, 334
Hin1II CATG 4 cut(s) 182, 296, 587, 617
HincII GTYRAC 1 cut(s) 409
HindII GTYRAC 1 cut(s) 409
HindIII AAGCTT 1 cut(s) 92
HinfI GANTC 7 cut(s) 35, 160, 380, 410, 416, 422, 455
HphI GGTGA 1 cut(s) 275
Hpy166II GTNNAC 1 cut(s) 409
Hpy188I TCNGA 8 cut(s) 40, 159, 385, 415, 421, 427, 460, 656
Hpy188III TCNNGA 3 cut(s) 168, 214, 371
Hpy8I GTNNAC 1 cut(s) 409
HpyAV CCTTC 2 cut(s) 122, 223
HpyCH4III ACNGT 1 cut(s) 440
HpyCH4IV ACGT 1 cut(s) 541
HpyCH4V TGCA 4 cut(s) 12, 296, 333, 634
HpyF3I CTNAG 3 cut(s) 141, 465, 526
HpySE526I ACGT 1 cut(s) 541
Hsp92II CATG 4 cut(s) 182, 296, 587, 617
Ksp22I TGATCA 1 cut(s) 388
Kzo9I GATC 2 cut(s) 312, 388
LpnPI CCDG 9 cut(s) 33, 60, 153, 211, 273, 300, 468, 504, 587
Lsp1109I GCAGC 2 cut(s) 24, 345
LweI GCATC 2 cut(s) 126, 621
MaeI CTAG 2 cut(s) 260, 474
MaeII ACGT 1 cut(s) 541
MaeIII GTNAC 2 cut(s) 521, 586
MalI GATC 2 cut(s) 314, 390
MboI GATC 2 cut(s) 312, 388
MboII GAAGA 6 cut(s) 30, 33, 44, 71, 146, 202
MflI RGATCY 1 cut(s) 312
MluCI AATT 4 cut(s) 41, 233, 497, 643
MlyI GAGTC 5 cut(s) 29, 154, 374, 404, 464
MnlI CCTC 5 cut(s) 19, 150, 357, 441, 503
Mph1103I ATGCAT 1 cut(s) 636
MseI TTAA 2 cut(s) 96, 567
MslI CAYNNNNRTG 2 cut(s) 183, 604
MspR9I CCNGG 2 cut(s) 48, 288
MvaI CCWGG 2 cut(s) 48, 288
NdeII GATC 2 cut(s) 312, 388
NlaIII CATG 4 cut(s) 182, 296, 587, 617
NlaIV GGNNCC 1 cut(s) 597
NmuCI GTSAC 1 cut(s) 586
NsiI ATGCAT 1 cut(s) 636
NspI RCATGY 2 cut(s) 182, 296
PfeI GAWTC 2 cut(s) 416, 422
PkrI GCNGC 2 cut(s) 14, 335
PleI GAGTC 5 cut(s) 29, 154, 374, 404, 463
PpsI GAGTC 5 cut(s) 29, 154, 374, 404, 463
Psp6I CCWGG 2 cut(s) 46, 286
PspGI CCWGG 2 cut(s) 46, 286
PspN4I GGNNCC 1 cut(s) 597
PspPI GGNCC 1 cut(s) 596
PstI CTGCAG 1 cut(s) 335
PsuI RGATCY 1 cut(s) 312
RseI CAYNNNNRTG 2 cut(s) 183, 604
SaqAI TTAA 2 cut(s) 96, 567
SatI GCNGC 2 cut(s) 13, 334
Sau3AI GATC 2 cut(s) 312, 388
Sau96I GGNCC 1 cut(s) 596
SchI GAGTC 5 cut(s) 29, 154, 374, 404, 464
ScrFI CCNGG 2 cut(s) 48, 288
SetI ASST 9 cut(s) 96, 261, 265, 433, 452, 487, 514, 520, 544
SfaNI GCATC 2 cut(s) 126, 621
SfcI CTRYAG 1 cut(s) 331
SinI GGWCC 1 cut(s) 596
SmiMI CAYNNNNRTG 2 cut(s) 183, 604
Sse9I AATT 4 cut(s) 41, 233, 497, 643
SsiI CCGC 1 cut(s) 201
SspI AATATT 1 cut(s) 196
SspMI CTAG 2 cut(s) 260, 474
StyD4I CCNGG 2 cut(s) 46, 286
StyI CCWWGG 2 cut(s) 473, 591
TaaI ACNGT 1 cut(s) 440
TaiI ACGT 1 cut(s) 544
TasI AATT 4 cut(s) 41, 233, 497, 643
TfiI GAWTC 2 cut(s) 416, 422
Tru1I TTAA 2 cut(s) 96, 567
Tru9I TTAA 2 cut(s) 96, 567
TscAI CASTG 5 cut(s) 152, 335, 445, 568, 606
TseFI GTSAC 1 cut(s) 586
TseI GCWGC 2 cut(s) 12, 333
Tsp45I GTSAC 1 cut(s) 586
TspDTI ATGAA 2 cut(s) 45, 630
TspRI CASTG 5 cut(s) 152, 335, 445, 568, 606
VpaK11BI GGWCC 1 cut(s) 596
XapI RAATTY 3 cut(s) 41, 233, 643
XceI RCATGY 2 cut(s) 182, 296
XcmI CCANNNNNNNNNTGG 1 cut(s) 598
XmaJI CCTAGG 1 cut(s) 473
XspI CTAG 2 cut(s) 260, 474
Zsp2I ATGCAT 1 cut(s) 636
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.