Rh2CG048300

B3 DNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0002871
Physical Location & Seq
Forward (+)
3849745 .. 3851572
1828 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG048300.1

Sequence Viewer

Length: 798 bp
ATGGGTTCTTGCAGCAGAAGAAGAGAGGATGAAGGCTCTGAAAATCCAGGCTTCTACAAGTGTGTTGATTACTCTGTTCTTTCAACAGGGAAGCTTAAAGTCCCCCAAGATTTTTGTGAGAATTTTGAAGTAAAGGTGCCTCATCAATGCCACCTTGAGACTTTTTCTGGATCTTGGCTTGTTGGTTTGAAAAGGATTAGTAGTGAGTTCTTCTTCAAAAAGGGTTGGAGGGAATTTGTAGAGGGAAATTGTTTAAAACTAGGTGAATATATGGTCTTGCAGTACATTGGACATGCAAACTTCAATGTTGAAATTTATGGGCTCAATCGCTGCAATAAGATGTCAAAGGCTAGGAAAAGTGACAGGCTTCTTGATGATTTTGATGTGATTGTTGTGGACTCTGATTCAGATTCTGAAATTGAGTTTCCTAGGGAACCGGTTTGTGGAAGAAAATTGTCAAGGAGAGGTAGACCTCGTAATTTAGCCACCGTAAACCGTTCTGGAAATCCCTGCTTCAGTGTCCAGATGTGCCAGACTCATGTGACCAAGGGACCATTGCTCACACCAAGGAGCTTCATGAGGAACACACACAAGCCAAAGAAAGTTGAGCTTCAAGTTGGAGACATGGTCTGGGAGGTGAACTGGATCCATCCTTACCAGAAGTCTAGTAGGTTCAGTGGAGGCTGGCTACGTTTTGTGAGCCATAACGGATTGCAAGAGGGAGATTTATGTGACTTTGAGCTAATATCGAAGACAAATGAGGTTGCGGTGATGAACGTTTCTATTAGGAAAATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

265

Amino Acids

30.51

Weight (kDa)

8.87

Isoelectric Point (pI)

45.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 24 - 105 1.9e-11 B3 DNA binding domain
B3 PF02362 174 - 254 1.9e-11 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 136
AccI GTMKAC 1 cut(s) 469
AciI CCGC 1 cut(s) 767
AclI AACGTT 1 cut(s) 777
AclWI GGATC 3 cut(s) 178, 640, 653
AcsI RAATTY 3 cut(s) 121, 233, 312
AcuI CTGAAG 1 cut(s) 499
AfaI GTAC 1 cut(s) 284
AfiI CCNNNNNNNGG 1 cut(s) 443
AgeI ACCGGT 1 cut(s) 436
AgsI TTSAA 7 cut(s) 84, 128, 190, 217, 304, 311, 614
AjnI CCWGG 1 cut(s) 46
AluBI AGCT 4 cut(s) 94, 573, 610, 742
AluI AGCT 4 cut(s) 94, 573, 610, 742
Alw26I GTCTC 2 cut(s) 152, 615
AlwI GGATC 3 cut(s) 178, 640, 653
AlwNI CAGNNNCTG 1 cut(s) 413
ApeKI GCWGC 2 cut(s) 12, 330
ApoI RAATTY 3 cut(s) 121, 233, 312
AsiGI ACCGGT 1 cut(s) 436
AspA2I CCTAGG 1 cut(s) 428
AspS9I GGNCC 1 cut(s) 551
AsuHPI GGTGA 3 cut(s) 275, 649, 781
AvaII GGWCC 1 cut(s) 551
AvrII CCTAGG 1 cut(s) 428
BamHI GGATCC 1 cut(s) 645
BanI GGYRCC 1 cut(s) 136
BanII GRGCYC 1 cut(s) 324
BbsI GAAGAC 1 cut(s) 758
BbvI GCAGC 2 cut(s) 24, 317
BccI CCATC 1 cut(s) 657
BciT130I CCWGG 1 cut(s) 48
BcoDI GTCTC 2 cut(s) 152, 615
BfaI CTAG 4 cut(s) 260, 351, 429, 666
BisI GCNGC 2 cut(s) 13, 331
BlnI CCTAGG 1 cut(s) 428
BlsI GCNGC 2 cut(s) 14, 332
Bme1390I CCNGG 1 cut(s) 48
Bme18I GGWCC 1 cut(s) 551
BmgT120I GGNCC 1 cut(s) 551
BmiI GGNNCC 4 cut(s) 138, 435, 552, 647
BmrFI CCNGG 1 cut(s) 48
BpiI GAAGAC 1 cut(s) 758
BpuEI CTTGAG 1 cut(s) 176
BsaJI CCNNGG 3 cut(s) 428, 546, 566
BsaWI WCCGGW 1 cut(s) 436
BsaXI ACNNNNNCTCC 2 cut(s) 612, 642
Bsc4I CCNNNNNNNGG 1 cut(s) 443
Bse118I RCCGGY 1 cut(s) 436
Bse1I ACTGG 1 cut(s) 647
Bse3DI GCAATG 1 cut(s) 554
BseBI CCWGG 1 cut(s) 48
BseDI CCNNGG 3 cut(s) 428, 546, 566
BseGI GGATG 2 cut(s) 34, 649
BseLI CCNNNNNNNGG 1 cut(s) 443
BseMI GCAATG 1 cut(s) 554
BseNI ACTGG 1 cut(s) 647
BseXI GCAGC 2 cut(s) 24, 317
BshNI GGYRCC 1 cut(s) 136
BshTI ACCGGT 1 cut(s) 436
BsiSI CCGG 1 cut(s) 437
BslFI GGGAC 2 cut(s) 86, 564
BslI CCNNNNNNNGG 1 cut(s) 443
BsmAI GTCTC 2 cut(s) 152, 615
BsmFI GGGAC 2 cut(s) 86, 564
Bsp1286I GDGCHC 1 cut(s) 324
Bsp143I GATC 2 cut(s) 170, 645
BspACI CCGC 1 cut(s) 767
BspHI TCATGA 1 cut(s) 576
BspLI GGNNCC 4 cut(s) 138, 435, 552, 647
BspPI GGATC 3 cut(s) 178, 640, 653
BspT107I GGYRCC 1 cut(s) 136
BsrDI GCAATG 1 cut(s) 554
BsrFI RCCGGY 1 cut(s) 436
BsrI ACTGG 1 cut(s) 647
BssAI RCCGGY 1 cut(s) 436
BssECI CCNNGG 3 cut(s) 428, 546, 566
BssMI GATC 2 cut(s) 170, 645
BssT1I CCWWGG 3 cut(s) 428, 546, 566
Bst2UI CCWGG 1 cut(s) 48
Bst4CI ACNGT 2 cut(s) 490, 497
Bst6I CTCTTC 1 cut(s) 16
BstC8I GCNNGC 1 cut(s) 686
BstF5I GGATG 2 cut(s) 34, 649
BstKTI GATC 2 cut(s) 173, 648
BstMAI GTCTC 2 cut(s) 152, 615
BstMBI GATC 2 cut(s) 170, 645
BstNI CCWGG 1 cut(s) 48
BstNSI RCATGY 1 cut(s) 296
BstSCI CCNGG 1 cut(s) 46
BstV1I GCAGC 2 cut(s) 24, 317
BstV2I GAAGAC 1 cut(s) 758
BstX2I RGATCY 2 cut(s) 170, 645
BstYI RGATCY 2 cut(s) 170, 645
BtsCI GGATG 2 cut(s) 34, 649
BtsIMutI CAGTG 2 cut(s) 523, 682
Cac8I GCNNGC 1 cut(s) 686
CaiI CAGNNNCTG 1 cut(s) 413
CciI TCATGA 1 cut(s) 576
Cfr10I RCCGGY 1 cut(s) 436
Cfr13I GGNCC 1 cut(s) 551
Csp6I GTAC 1 cut(s) 283
CspAI ACCGGT 1 cut(s) 436
CviAII CATG 4 cut(s) 293, 539, 577, 625
CviQI GTAC 1 cut(s) 283
DpnI GATC 2 cut(s) 172, 647
DpnII GATC 2 cut(s) 170, 645
DraI TTTAAA 1 cut(s) 255
Eam1104I CTCTTC 1 cut(s) 16
EarI CTCTTC 1 cut(s) 16
Eco130I CCWWGG 3 cut(s) 428, 546, 566
Eco24I GRGCYC 1 cut(s) 324
Eco47I GGWCC 1 cut(s) 551
Eco57I CTGAAG 1 cut(s) 499
EcoRII CCWGG 1 cut(s) 46
EcoT14I CCWWGG 3 cut(s) 428, 546, 566
EcoT38I GRGCYC 1 cut(s) 324
ErhI CCWWGG 3 cut(s) 428, 546, 566
FaeI CATG 4 cut(s) 296, 542, 580, 628
FaiI YATR 9 cut(s) 270, 272, 294, 318, 540, 578, 626, 705, 730
FalI AAGNNNNNCTT 2 cut(s) 594, 626
FaqI GGGAC 2 cut(s) 86, 564
FatI CATG 4 cut(s) 292, 538, 576, 624
FblI GTMKAC 1 cut(s) 469
Fnu4HI GCNGC 2 cut(s) 13, 331
FokI GGATG 2 cut(s) 41, 636
FriOI GRGCYC 1 cut(s) 324
Fsp4HI GCNGC 2 cut(s) 13, 331
FspBI CTAG 4 cut(s) 260, 351, 429, 666
GluI GCNGC 2 cut(s) 13, 331
HapII CCGG 1 cut(s) 437
Hin1II CATG 4 cut(s) 296, 542, 580, 628
HindIII AAGCTT 1 cut(s) 92
HinfI GANTC 4 cut(s) 398, 404, 410, 535
HpaII CCGG 1 cut(s) 437
HphI GGTGA 3 cut(s) 275, 649, 781
Hpy166II GTNNAC 4 cut(s) 397, 470, 493, 640
Hpy188I TCNGA 4 cut(s) 40, 403, 409, 415
Hpy188III TCNNGA 5 cut(s) 168, 371, 501, 523, 577
Hpy8I GTNNAC 4 cut(s) 397, 470, 493, 640
HpyAV CCTTC 1 cut(s) 26
HpyCH4III ACNGT 2 cut(s) 490, 497
HpyCH4IV ACGT 2 cut(s) 691, 777
HpyCH4V TGCA 5 cut(s) 12, 280, 296, 333, 715
HpySE526I ACGT 2 cut(s) 691, 777
Hsp92II CATG 4 cut(s) 296, 542, 580, 628
Kzo9I GATC 2 cut(s) 170, 645
LmnI GCTCC 1 cut(s) 570
Lsp1109I GCAGC 2 cut(s) 24, 317
MaeI CTAG 4 cut(s) 260, 351, 429, 666
MaeII ACGT 2 cut(s) 691, 777
MaeIII GTNAC 3 cut(s) 359, 541, 731
MalI GATC 2 cut(s) 172, 647
MboI GATC 2 cut(s) 170, 645
MboII GAAGA 6 cut(s) 30, 33, 202, 205, 459, 763
MflI RGATCY 2 cut(s) 170, 645
MhlI GDGCHC 1 cut(s) 324
MluCI AATT 7 cut(s) 121, 233, 247, 312, 417, 452, 478
MlyI GAGTC 2 cut(s) 392, 529
MmeI TCCRAC 2 cut(s) 206, 598
MseI TTAA 2 cut(s) 96, 254
MspI CCGG 1 cut(s) 437
MspR9I CCNGG 1 cut(s) 48
MvaI CCWGG 1 cut(s) 48
NdeII GATC 2 cut(s) 170, 645
NlaIII CATG 4 cut(s) 296, 542, 580, 628
NlaIV GGNNCC 4 cut(s) 138, 435, 552, 647
NmuCI GTSAC 3 cut(s) 359, 541, 731
NspI RCATGY 1 cut(s) 296
PagI TCATGA 1 cut(s) 576
PfeI GAWTC 2 cut(s) 404, 410
PflFI GACNNNGTC 1 cut(s) 626
PinAI ACCGGT 1 cut(s) 436
PkrI GCNGC 2 cut(s) 14, 332
PleI GAGTC 2 cut(s) 392, 529
PpsI GAGTC 2 cut(s) 392, 529
Psp1406I AACGTT 1 cut(s) 777
Psp6I CCWGG 1 cut(s) 46
PspGI CCWGG 1 cut(s) 46
PspN4I GGNNCC 4 cut(s) 138, 435, 552, 647
PspPI GGNCC 1 cut(s) 551
PstNI CAGNNNCTG 1 cut(s) 413
PsuI RGATCY 2 cut(s) 170, 645
PsyI GACNNNGTC 1 cut(s) 626
RsaI GTAC 1 cut(s) 284
RsaNI GTAC 1 cut(s) 283
SaqAI TTAA 2 cut(s) 96, 254
SatI GCNGC 2 cut(s) 13, 331
Sau3AI GATC 2 cut(s) 170, 645
Sau96I GGNCC 1 cut(s) 551
SchI GAGTC 2 cut(s) 392, 529
ScrFI CCNGG 1 cut(s) 48
SduI GDGCHC 1 cut(s) 324
SinI GGWCC 1 cut(s) 551
SmlI CTYRAG 1 cut(s) 155
SmoI CTYRAG 1 cut(s) 155
Sse9I AATT 7 cut(s) 121, 233, 247, 312, 417, 452, 478
SsiI CCGC 1 cut(s) 767
SspMI CTAG 4 cut(s) 260, 351, 429, 666
StyD4I CCNGG 1 cut(s) 46
StyI CCWWGG 3 cut(s) 428, 546, 566
TaaI ACNGT 2 cut(s) 490, 497
TaiI ACGT 2 cut(s) 694, 780
TaqI TCGA 1 cut(s) 749
TasI AATT 7 cut(s) 121, 233, 247, 312, 417, 452, 478
TatI WGTACW 1 cut(s) 282
TfiI GAWTC 2 cut(s) 404, 410
Tru1I TTAA 2 cut(s) 96, 254
Tru9I TTAA 2 cut(s) 96, 254
TscAI CASTG 2 cut(s) 523, 682
TseFI GTSAC 3 cut(s) 359, 541, 731
TseI GCWGC 2 cut(s) 12, 330
Tsp45I GTSAC 3 cut(s) 359, 541, 731
TspDTI ATGAA 3 cut(s) 45, 565, 788
TspGWI ACGGA 1 cut(s) 723
TspRI CASTG 2 cut(s) 523, 682
Tth111I GACNNNGTC 1 cut(s) 626
VpaK11BI GGWCC 1 cut(s) 551
XapI RAATTY 3 cut(s) 121, 233, 312
XceI RCATGY 1 cut(s) 296
XmaJI CCTAGG 1 cut(s) 428
XmiI GTMKAC 1 cut(s) 469
XspI CTAG 4 cut(s) 260, 351, 429, 666
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.