Rroxscaffold_2G00151690

B3 DNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0002871
Physical Location & Seq
Reverse (-)
88880576 .. 88885772
5197 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00151690.1

Sequence Viewer

Length: 1005 bp
ATGGTCGCTCCGGCGGCCGGGCCACCACCTCCATCAAGTCTCCACCGCCATCGAGAGATGATCTCAACGATCATGGGTCCCCCAAGCCGGCCCCAAGATCGGAGCACCGTCGATTCGAACTCAACCTCCGAGCGCCGCCCCTTGCTGTTTCTGGTGCCCAAAGGAGCAAAGATCCCAAAAAGAAGAGGCAGCAAAGCCAACTCTGTGATGGGTTCTTGCAGCAGAAGAAGAGAGGATGAAGGCTCTGAAAATCCAGGCTTCTACAAGTGTGTTGATTACTCTGTTCTTTCAACAGGGAAGCTTAAAATCCCCCAAGATTTTTGTGAGAATTTTGAAGTAAAGGTGCCTCATCAGTGCCACCTTGAGACTTTTTCCGGATCTTGGCTTGTTGGTTTGAAAAGGATTAGTGGAGAGTTCTTCTTCAAAAAGAGTTGGAGGGAATTTGTAGAGGGAAATTGTTTGAAACTAGGTGAATATATGATCTTGCAGTACATTGGACGTGCAAAGTTCAATGTTGAAATTTATGGGCTCAATCGTTGCAATAAGATGTCAAAGGCTAGGAAAAGTGACAGGCTTATTGATGATCATGATGTGATTGTTGTGGACTCTGATTCAGATTCTGAAATTGAGTTTCCTAGGAAACCGGTTTGTGGAAGAAAATTGTCAAGGAGAGGTAGACCTCGTAATCTAGCCACCGTAAACCGTTCGGGAAATCCCTGCTTCAGTGTCCAGATGTGCCAGACTCATGTGACCAAGGGACCTTTGCTCACACCAAGGAGCTTCATGAGGAACACACACAAGCCAAAGAAAGTTGAGCTTCAAGTTGGAGACATGGTCTGGGATGTGAACTGGATCCATCCTTACCAGAAGTCTAGTAGGTTCAGTGGAGGCTGGCTACGTTTCGTGAGCCATAACGAATTGCAAGAGGGAGATTTCTGTGACTTTGAGCTGATATCGAAGACAAATGAGGTTGCGGTGATGAACGTTTCTATTAGGAAAATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

334

Amino Acids

38.09

Weight (kDa)

9.6

Isoelectric Point (pI)

50.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 93 - 174 1.6e-10 B3 DNA binding domain
B3 PF02362 243 - 323 2.4e-11 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 154, 343
AccI GTMKAC 1 cut(s) 676
AccIII TCCGGA 1 cut(s) 374
AciI CCGC 4 cut(s) 14, 46, 136, 974
AclI AACGTT 1 cut(s) 984
AclWI GGATC 4 cut(s) 166, 385, 847, 860
AcoI YGGCCR 1 cut(s) 15
AcsI RAATTY 3 cut(s) 328, 440, 519
AcuI CTGAAG 1 cut(s) 706
AfaI GTAC 1 cut(s) 491
AfiI CCNNNNNNNGG 5 cut(s) 17, 87, 99, 381, 650
AgeI ACCGGT 1 cut(s) 643
AgsI TTSAA 8 cut(s) 291, 335, 397, 424, 463, 511, 518, 821
AjiI CACGTC 1 cut(s) 500
AjnI CCWGG 1 cut(s) 253
AloI GAACNNNNNNTCC 2 cut(s) 110, 142
AluBI AGCT 4 cut(s) 301, 780, 817, 949
AluI AGCT 4 cut(s) 301, 780, 817, 949
Alw21I GWGCWC 1 cut(s) 107
Alw26I GTCTC 3 cut(s) 44, 359, 822
AlwI GGATC 4 cut(s) 166, 385, 847, 860
AlwNI CAGNNNCTG 1 cut(s) 620
Aor13HI TCCGGA 1 cut(s) 374
AoxI GGCC 3 cut(s) 15, 20, 89
ApeKI GCWGC 2 cut(s) 189, 219
ApoI RAATTY 3 cut(s) 328, 440, 519
AsiGI ACCGGT 1 cut(s) 643
AspA2I CCTAGG 1 cut(s) 635
AspLEI GCGC 1 cut(s) 135
AspS9I GGNCC 4 cut(s) 20, 77, 90, 758
AsuC2I CCSGG 1 cut(s) 19
AsuHPI GGTGA 2 cut(s) 482, 988
AsuII TTCGAA 1 cut(s) 116
AvaII GGWCC 2 cut(s) 77, 758
AvrII CCTAGG 1 cut(s) 635
BaeGI GKGCMC 1 cut(s) 159
BamHI GGATCC 1 cut(s) 852
BanI GGYRCC 2 cut(s) 154, 343
BanII GRGCYC 1 cut(s) 531
BbsI GAAGAC 1 cut(s) 965
Bbv12I GWGCWC 1 cut(s) 107
BbvI GCAGC 2 cut(s) 201, 231
BccI CCATC 4 cut(s) 40, 57, 202, 864
BciT130I CCWGG 1 cut(s) 255
BclI TGATCA 1 cut(s) 583
BcnI CCSGG 1 cut(s) 19
BcoDI GTCTC 3 cut(s) 44, 359, 822
BfaI CTAG 5 cut(s) 467, 558, 636, 689, 873
BfoI RGCGCY 1 cut(s) 136
BisI GCNGC 4 cut(s) 15, 136, 190, 220
BlnI CCTAGG 1 cut(s) 635
BlsI GCNGC 4 cut(s) 16, 137, 191, 221
Bme1390I CCNGG 2 cut(s) 19, 255
Bme18I GGWCC 2 cut(s) 77, 758
BmgBI CACGTC 1 cut(s) 500
BmgT120I GGNCC 4 cut(s) 20, 77, 90, 758
BmiI GGNNCC 7 cut(s) 78, 79, 92, 156, 345, 759, 854
BmrFI CCNGG 2 cut(s) 19, 255
BpiI GAAGAC 1 cut(s) 965
BplI GAGNNNNNCTC 2 cut(s) 47, 79
Bpu14I TTCGAA 1 cut(s) 116
BpuEI CTTGAG 1 cut(s) 383
BpuMI CCSGG 1 cut(s) 19
BsaJI CCNNGG 3 cut(s) 635, 753, 773
BsaWI WCCGGW 2 cut(s) 374, 643
BsaXI ACNNNNNCTCC 4 cut(s) 110, 140, 819, 849
Bsc4I CCNNNNNNNGG 5 cut(s) 17, 87, 99, 381, 650
Bse118I RCCGGY 2 cut(s) 87, 643
Bse1I ACTGG 1 cut(s) 854
BseAI TCCGGA 1 cut(s) 374
BseBI CCWGG 1 cut(s) 255
BseDI CCNNGG 3 cut(s) 635, 753, 773
BseGI GGATG 3 cut(s) 241, 847, 856
BseLI CCNNNNNNNGG 5 cut(s) 17, 87, 99, 381, 650
BseNI ACTGG 1 cut(s) 854
BseSI GKGCMC 1 cut(s) 159
BseX3I CGGCCG 1 cut(s) 15
BseXI GCAGC 2 cut(s) 201, 231
Bsh1285I CGRYCG 1 cut(s) 18
BshFI GGCC 3 cut(s) 17, 22, 91
BshNI GGYRCC 2 cut(s) 154, 343
BshTI ACCGGT 1 cut(s) 643
BsiEI CGRYCG 1 cut(s) 18
BsiHKAI GWGCWC 1 cut(s) 107
BsiSI CCGG 5 cut(s) 11, 18, 88, 375, 644
BslFI GGGAC 2 cut(s) 63, 771
BslI CCNNNNNNNGG 5 cut(s) 17, 87, 99, 381, 650
BsmAI GTCTC 3 cut(s) 44, 359, 822
BsmFI GGGAC 2 cut(s) 63, 771
BsnI GGCC 3 cut(s) 17, 22, 91
Bsp119I TTCGAA 1 cut(s) 116
Bsp1286I GDGCHC 3 cut(s) 107, 159, 531
Bsp13I TCCGGA 1 cut(s) 374
Bsp143I GATC 8 cut(s) 60, 69, 97, 171, 377, 480, 583, 852
BspACI CCGC 4 cut(s) 14, 46, 136, 974
BspANI GGCC 3 cut(s) 17, 22, 91
BspEI TCCGGA 1 cut(s) 374
BspHI TCATGA 2 cut(s) 586, 783
BspLI GGNNCC 7 cut(s) 78, 79, 92, 156, 345, 759, 854
BspPI GGATC 4 cut(s) 166, 385, 847, 860
BspT104I TTCGAA 1 cut(s) 116
BspT107I GGYRCC 2 cut(s) 154, 343
BsrFI RCCGGY 2 cut(s) 87, 643
BsrI ACTGG 1 cut(s) 854
BssAI RCCGGY 2 cut(s) 87, 643
BssECI CCNNGG 3 cut(s) 635, 753, 773
BssMI GATC 8 cut(s) 60, 69, 97, 171, 377, 480, 583, 852
BssT1I CCWWGG 3 cut(s) 635, 753, 773
Bst2UI CCWGG 1 cut(s) 255
Bst4CI ACNGT 3 cut(s) 109, 697, 704
Bst6I CTCTTC 2 cut(s) 178, 223
BstBI TTCGAA 1 cut(s) 116
BstC8I GCNNGC 2 cut(s) 89, 893
BstF5I GGATG 3 cut(s) 241, 847, 856
BstH2I RGCGCY 1 cut(s) 136
BstHHI GCGC 1 cut(s) 135
BstKTI GATC 8 cut(s) 63, 72, 100, 174, 380, 483, 586, 855
BstMAI GTCTC 3 cut(s) 44, 359, 822
BstMBI GATC 8 cut(s) 60, 69, 97, 171, 377, 480, 583, 852
BstMCI CGRYCG 1 cut(s) 18
BstMWI GCNNNNNNNGC 1 cut(s) 14
BstNI CCWGG 1 cut(s) 255
BstSCI CCNGG 2 cut(s) 17, 253
BstSLI GKGCMC 1 cut(s) 159
BstV1I GCAGC 2 cut(s) 201, 231
BstV2I GAAGAC 1 cut(s) 965
BstX2I RGATCY 3 cut(s) 171, 377, 852
BstYI RGATCY 3 cut(s) 171, 377, 852
BstZI CGGCCG 1 cut(s) 15
BsuRI GGCC 3 cut(s) 17, 22, 91
BtrI CACGTC 1 cut(s) 500
BtsCI GGATG 3 cut(s) 241, 847, 856
BtsIMutI CAGTG 3 cut(s) 359, 730, 889
Cac8I GCNNGC 2 cut(s) 89, 893
CaiI CAGNNNCTG 1 cut(s) 620
CciI TCATGA 2 cut(s) 586, 783
CfoI GCGC 1 cut(s) 135
Cfr10I RCCGGY 2 cut(s) 87, 643
Cfr13I GGNCC 4 cut(s) 20, 77, 90, 758
Csp6I GTAC 1 cut(s) 490
CspAI ACCGGT 1 cut(s) 643
CviAII CATG 5 cut(s) 73, 587, 746, 784, 832
CviQI GTAC 1 cut(s) 490
DpnI GATC 8 cut(s) 62, 71, 99, 173, 379, 482, 585, 854
DpnII GATC 8 cut(s) 60, 69, 97, 171, 377, 480, 583, 852
EaeI YGGCCR 1 cut(s) 15
EagI CGGCCG 1 cut(s) 15
Eam1104I CTCTTC 2 cut(s) 178, 223
EarI CTCTTC 2 cut(s) 178, 223
EclXI CGGCCG 1 cut(s) 15
Eco130I CCWWGG 3 cut(s) 635, 753, 773
Eco24I GRGCYC 1 cut(s) 531
Eco32I GATATC 1 cut(s) 954
Eco47I GGWCC 2 cut(s) 77, 758
Eco52I CGGCCG 1 cut(s) 15
Eco57I CTGAAG 1 cut(s) 706
EcoO109I RGGNCCY 2 cut(s) 77, 758
EcoRII CCWGG 1 cut(s) 253
EcoRV GATATC 1 cut(s) 954
EcoT14I CCWWGG 3 cut(s) 635, 753, 773
EcoT38I GRGCYC 1 cut(s) 531
ErhI CCWWGG 3 cut(s) 635, 753, 773
FaeI CATG 5 cut(s) 76, 590, 749, 787, 835
FaiI YATR 9 cut(s) 74, 477, 479, 525, 588, 747, 785, 833, 912
FalI AAGNNNNNCTT 2 cut(s) 801, 833
FaqI GGGAC 2 cut(s) 63, 771
FatI CATG 5 cut(s) 72, 586, 745, 783, 831
FbaI TGATCA 1 cut(s) 583
FblI GTMKAC 1 cut(s) 676
Fnu4HI GCNGC 4 cut(s) 15, 136, 190, 220
FokI GGATG 3 cut(s) 248, 843, 854
FriOI GRGCYC 1 cut(s) 531
Fsp4HI GCNGC 4 cut(s) 15, 136, 190, 220
FspBI CTAG 5 cut(s) 467, 558, 636, 689, 873
GlaI GCGC 1 cut(s) 134
GluI GCNGC 4 cut(s) 15, 136, 190, 220
HaeII RGCGCY 1 cut(s) 136
HaeIII GGCC 3 cut(s) 17, 22, 91
HapII CCGG 5 cut(s) 11, 18, 88, 375, 644
HhaI GCGC 1 cut(s) 135
Hin1II CATG 5 cut(s) 76, 590, 749, 787, 835
Hin6I GCGC 1 cut(s) 133
HinP1I GCGC 1 cut(s) 133
HindIII AAGCTT 1 cut(s) 299
HinfI GANTC 5 cut(s) 113, 605, 611, 617, 742
HpaII CCGG 5 cut(s) 11, 18, 88, 375, 644
HphI GGTGA 2 cut(s) 482, 988
Hpy166II GTNNAC 4 cut(s) 604, 677, 700, 847
Hpy188I TCNGA 6 cut(s) 102, 130, 247, 610, 616, 622
Hpy188III TCNNGA 7 cut(s) 53, 375, 587, 708, 730, 784, 904
Hpy8I GTNNAC 4 cut(s) 604, 677, 700, 847
Hpy99I CGWCG 1 cut(s) 113
HpyAV CCTTC 1 cut(s) 233
HpyCH4III ACNGT 3 cut(s) 109, 697, 704
HpyCH4IV ACGT 3 cut(s) 499, 898, 984
HpyCH4V TGCA 5 cut(s) 219, 487, 503, 540, 922
HpyF10VI GCNNNNNNNGC 1 cut(s) 14
HpySE526I ACGT 3 cut(s) 499, 898, 984
Hsp92II CATG 5 cut(s) 76, 590, 749, 787, 835
HspAI GCGC 1 cut(s) 133
KflI GGGWCCC 1 cut(s) 77
Kpn2I TCCGGA 1 cut(s) 374
KroI GCCGGC 1 cut(s) 87
KroNI GCCGGC 1 cut(s) 89
Ksp22I TGATCA 1 cut(s) 583
Kzo9I GATC 8 cut(s) 60, 69, 97, 171, 377, 480, 583, 852
LmnI GCTCC 4 cut(s) 13, 102, 164, 777
Lsp1109I GCAGC 2 cut(s) 201, 231
MaeI CTAG 5 cut(s) 467, 558, 636, 689, 873
MaeII ACGT 3 cut(s) 499, 898, 984
MaeIII GTNAC 3 cut(s) 566, 748, 938
MalI GATC 8 cut(s) 62, 71, 99, 173, 379, 482, 585, 854
MboI GATC 8 cut(s) 60, 69, 97, 171, 377, 480, 583, 852
MboII GAAGA 7 cut(s) 195, 237, 240, 409, 412, 666, 970
MflI RGATCY 3 cut(s) 171, 377, 852
MhlI GDGCHC 3 cut(s) 107, 159, 531
MluCI AATT 7 cut(s) 328, 440, 454, 519, 624, 659, 917
MlyI GAGTC 2 cut(s) 599, 736
MmeI TCCRAC 2 cut(s) 413, 805
MroI TCCGGA 1 cut(s) 374
MroNI GCCGGC 1 cut(s) 87
MseI TTAA 1 cut(s) 303
MspI CCGG 5 cut(s) 11, 18, 88, 375, 644
MspR9I CCNGG 2 cut(s) 19, 255
MvaI CCWGG 1 cut(s) 255
MwoI GCNNNNNNNGC 1 cut(s) 14
NaeI GCCGGC 1 cut(s) 89
NciI CCSGG 1 cut(s) 19
NdeII GATC 8 cut(s) 60, 69, 97, 171, 377, 480, 583, 852
NgoMIV GCCGGC 1 cut(s) 87
NlaIII CATG 5 cut(s) 76, 590, 749, 787, 835
NlaIV GGNNCC 7 cut(s) 78, 79, 92, 156, 345, 759, 854
NmuCI GTSAC 3 cut(s) 566, 748, 938
NspV TTCGAA 1 cut(s) 116
PagI TCATGA 2 cut(s) 586, 783
PdiI GCCGGC 1 cut(s) 89
PfeI GAWTC 3 cut(s) 113, 611, 617
PflFI GACNNNGTC 1 cut(s) 833
PinAI ACCGGT 1 cut(s) 643
PkrI GCNGC 4 cut(s) 16, 137, 191, 221
PleI GAGTC 2 cut(s) 599, 736
PpsI GAGTC 2 cut(s) 599, 736
PpuMI RGGWCCY 2 cut(s) 77, 758
Psp1406I AACGTT 1 cut(s) 984
Psp5II RGGWCCY 2 cut(s) 77, 758
Psp6I CCWGG 1 cut(s) 253
PspGI CCWGG 1 cut(s) 253
PspN4I GGNNCC 7 cut(s) 78, 79, 92, 156, 345, 759, 854
PspPI GGNCC 4 cut(s) 20, 77, 90, 758
PspPPI RGGWCCY 2 cut(s) 77, 758
PstNI CAGNNNCTG 1 cut(s) 620
PsuI RGATCY 3 cut(s) 171, 377, 852
PsyI GACNNNGTC 1 cut(s) 833
RsaI GTAC 1 cut(s) 491
RsaNI GTAC 1 cut(s) 490
SaqAI TTAA 1 cut(s) 303
SatI GCNGC 4 cut(s) 15, 136, 190, 220
Sau3AI GATC 8 cut(s) 60, 69, 97, 171, 377, 480, 583, 852
Sau96I GGNCC 4 cut(s) 20, 77, 90, 758
SchI GAGTC 2 cut(s) 599, 736
ScrFI CCNGG 2 cut(s) 19, 255
SduI GDGCHC 3 cut(s) 107, 159, 531
SfuI TTCGAA 1 cut(s) 116
SinI GGWCC 2 cut(s) 77, 758
SmlI CTYRAG 1 cut(s) 362
SmoI CTYRAG 1 cut(s) 362
Sse9I AATT 7 cut(s) 328, 440, 454, 519, 624, 659, 917
SsiI CCGC 4 cut(s) 14, 46, 136, 974
SspMI CTAG 5 cut(s) 467, 558, 636, 689, 873
StyD4I CCNGG 2 cut(s) 17, 253
StyI CCWWGG 3 cut(s) 635, 753, 773
TaaI ACNGT 3 cut(s) 109, 697, 704
TaiI ACGT 3 cut(s) 502, 901, 987
TaqI TCGA 4 cut(s) 52, 111, 116, 956
TasI AATT 7 cut(s) 328, 440, 454, 519, 624, 659, 917
TatI WGTACW 1 cut(s) 489
TauI GCSGC 2 cut(s) 17, 138
TfiI GAWTC 3 cut(s) 113, 611, 617
Tru1I TTAA 1 cut(s) 303
Tru9I TTAA 1 cut(s) 303
TscAI CASTG 3 cut(s) 359, 730, 889
TseFI GTSAC 3 cut(s) 566, 748, 938
TseI GCWGC 2 cut(s) 189, 219
Tsp45I GTSAC 3 cut(s) 566, 748, 938
TspDTI ATGAA 3 cut(s) 252, 772, 995
TspRI CASTG 3 cut(s) 359, 730, 889
Tth111I GACNNNGTC 1 cut(s) 833
VpaK11BI GGWCC 2 cut(s) 77, 758
XapI RAATTY 3 cut(s) 328, 440, 519
XcmI CCANNNNNNNNNTGG 1 cut(s) 205
XmaJI CCTAGG 1 cut(s) 635
XmiI GTMKAC 1 cut(s) 676
XspI CTAG 5 cut(s) 467, 558, 636, 689, 873
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.