RLG00000017994

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0003968
Physical Location & Seq
Reverse (-)
24219897 .. 24223922
4026 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017994

Sequence Viewer

Length: 1887 bp
ATGGTCATAGAGGAGTCAGCTGATGAGTACTGGAGATATGAATTTAGGGTTTATGGGACCCCTGAATTTCATAGTGAGGGTTTTACTCTAGAAATACACCATGGAAGGAGTTTTGTTGATGCAGGTGGTGGGCAACATAAATACATTGGAGGTGAAGTTCATTGGCTTGAAAAGCTTGATCCAGAAAAGACTTCATGGTTGGAGCTCAACACATTTGCTTGGAGACTTGGTTATAGGGAACCACCAGTTCACTACTGGTTCAAGCACCCATATTTGTCATGGTACATTCCCGTAAAAGATGACAAAGATACAGCCAAGATGATTAAATCTTTGCCAAAAAATAGAATTGTGATCATTTATTTTGTTGGTGGAGGTGTTAGGAGGGTAGAAGAAGCAGTTGCAGAGTTAGAGAAGGATGACTATGAGAAAATAATACCGCACATAAGGTTCAAGCCAGAGATAATTATGGCTAGACTAAGGGCTGTTGACGTTGGTCAAAGTTCTTTTGGTCATACAAGTGCATTTGAATATCCAATTTCTCAATTGACTGAAGGTTCATTTGGTGGTCTAAGTAGTCAAACAATGGGGCATGGTGTTCTTTACCTTACTGATGAGCTAAATGGAGTAAAAGGTGTAGAACAAGACAAAACAGATGATGTAGGAGACTTGAGCAAGTTTGTGGATCATGTTGAAGAAGAATTTGAAGATGAAGACTCACAAGATAAGGACTATGTACCTCCAGACCCTTCGGAACCACCAAAGGAAGTGCTAGTTGATAGTGATTATGAAATCTTGCCAGAAGATGATAATGAGTTAGTACATGTGGTGGATGAAAGACAAGATGATGCAGGCTTTAATAAACATGTTGACAATGAAGATGTCTTTGAAGAGTTCCATGACATGGGATATGCAGGTTACATCTCAGACCATTCTAATGATAGTGAAGAGTTGAGGGAGAAAGATGAATTAGATTCAGATTCAGATGAGGCAGCAAGTAACAGAAAGAAGAAGAAAAAGGTTAAGCTAGGTAGGGCATGGGTTCTAGAAAGGGATATGAAAGACCCAAAATTTGAGGTTGGCCAACATTTTGCCAACTCCAAACAGTTCAAGGAAGCTGTGAAGAAATACGCAGTGAAGAATGGGTTGAATTTATCTTTCAATAAGAACACAGCTAAGAAAGGTAAGTTGGAAGATAGGCCTTGTCAATTTTGGATATATGTTGGATTTGTAAACAAGATATCTCCAGAGTTGATGATTAAAACTTTGAACTTGGAACATGGTGTTTGTGAGACGCTTCCAAGGGTTAAGATGTGCACATCTACATTTCTTGCAAAAGAGTATGTTAACCATTTCAAGGCAGATCCATATATGTCCAAGAAGTTGTTTCAAGAATTGGTGAATGAAGATTTTGGGCAGTCTATTTCACACAAGCAAGAACCACCTGCCGCAAGTGAAGCAAGGCCAACCAAGCTTCCTAGGACTAGATATGTGACTATGAGTTGTACAGTTTGTGGATCTGGGGATCACAACAGAAGAACCTGCCATATTGAACTTGCAAGGAAGGCCAGAGGAATTGGGGGACAAAGTAGTAGAGTTTCTGGAAATGTAAGGGTGAATGTGGATGTGAGTGTTGGAAAAAAGGGTGCTAACAATGTGAATGTACAAGTACAAACAGAAGCCGCACCAAAGGCCAAGAGAGCACCAAAGGCATCAAGGGCACCTTCACCAAGAGTACCTAGGAGGGGAGGCCATACATTAGCCTCTGTTGCCTCTTCTCAAAGGTCAAGTAGTACTGCCCCTGCATCATCTTCTCAAGCAACAGATTCATCACATGCAAGTAAAAGGGCAACCAGACAACCTGTTAAAAAGGCAATATGGAAGAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DBD_Tnp_Mut MULE SWIM

Protein Analysis

629

Amino Acids

71.03

Weight (kDa)

6.25

Isoelectric Point (pI)

48.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1-like PF26130 26 - 121 4.9e-15 PB1-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 113, 1450
Acc36I ACCTGC 4 cut(s) 113, 902, 1450, 1547
AccB1I GGYRCC 1 cut(s) 1717
AccB7I CCANNNNNTGG 1 cut(s) 901
AciI CCGC 3 cut(s) 437, 1446, 1680
AclWI GGATC 5 cut(s) 173, 690, 1355, 1522, 1530
AcoI YGGCCR 1 cut(s) 1078
AcsI RAATTY 5 cut(s) 41, 65, 698, 1067, 1147
AcuI CTGAAG 1 cut(s) 570
AfaI GTAC 9 cut(s) 29, 284, 735, 819, 1504, 1662, 1668, 1734, 1792
AfiI CCNNNNNNNGG 3 cut(s) 901, 1354, 1742
AflIII ACRYGT 2 cut(s) 820, 862
AjuI GAANNNNNNNTTGG 4 cut(s) 543, 575, 1169, 1201
AloI GAACNNNNNNTCC 2 cut(s) 141, 173
AluBI AGCT 8 cut(s) 20, 175, 205, 616, 1024, 1115, 1172, 1471
AluI AGCT 8 cut(s) 20, 175, 205, 616, 1024, 1115, 1172, 1471
Alw21I GWGCWC 3 cut(s) 207, 1316, 1702
Alw26I GTCTC 3 cut(s) 217, 657, 1283
Alw44I GTGCAC 1 cut(s) 1312
AlwI GGATC 5 cut(s) 173, 690, 1355, 1522, 1530
AoxI GGCC 6 cut(s) 1078, 1196, 1460, 1563, 1689, 1747
ApaLI GTGCAC 1 cut(s) 1312
ApeKI GCWGC 1 cut(s) 989
ApoI RAATTY 5 cut(s) 41, 65, 698, 1067, 1147
ArsI GACNNNNNNTTYG 2 cut(s) 1052, 1084
AspA2I CCTAGG 2 cut(s) 1475, 1736
AspS9I GGNCC 1 cut(s) 57
AsuHPI GGTGA 4 cut(s) 164, 1408, 1624, 1716
AvaII GGWCC 1 cut(s) 57
AvrII CCTAGG 2 cut(s) 1475, 1736
BaeGI GKGCMC 2 cut(s) 1316, 1720
BalI TGGCCA 1 cut(s) 1080
BanI GGYRCC 1 cut(s) 1717
BanII GRGCYC 1 cut(s) 207
BbsI GAAGAC 1 cut(s) 717
Bbv12I GWGCWC 3 cut(s) 207, 1316, 1702
BbvI GCAGC 1 cut(s) 1001
BclI TGATCA 1 cut(s) 351
BcoDI GTCTC 3 cut(s) 217, 657, 1283
BfaI CTAG 8 cut(s) 89, 471, 770, 1025, 1043, 1476, 1482, 1737
BfuAI ACCTGC 4 cut(s) 113, 902, 1450, 1547
BisI GCNGC 3 cut(s) 990, 1446, 1680
BlnI CCTAGG 2 cut(s) 1475, 1736
BlsI GCNGC 3 cut(s) 991, 1447, 1681
BmcAI AGTACT 2 cut(s) 29, 1792
Bme18I GGWCC 1 cut(s) 57
BmgT120I GGNCC 1 cut(s) 57
BmiI GGNNCC 5 cut(s) 58, 59, 240, 753, 1719
BmsI GCATC 4 cut(s) 109, 835, 1718, 1811
BoxI GACNNNNGTC 1 cut(s) 492
BpiI GAAGAC 1 cut(s) 717
BpmI CTGGAG 3 cut(s) 52, 723, 1227
BpuEI CTTGAG 2 cut(s) 688, 1797
BsaJI CCNNGG 4 cut(s) 100, 1298, 1475, 1736
BsaXI ACNNNNNCTCC 4 cut(s) 141, 171, 214, 244
Bsc4I CCNNNNNNNGG 3 cut(s) 901, 1354, 1742
Bse1I ACTGG 3 cut(s) 35, 245, 260
BseDI CCNNGG 4 cut(s) 100, 1298, 1475, 1736
BseGI GGATG 3 cut(s) 421, 835, 1627
BseLI CCNNNNNNNGG 3 cut(s) 901, 1354, 1742
BseMII CTCAG 1 cut(s) 936
BseNI ACTGG 3 cut(s) 35, 245, 260
BseRI GAGGAG 1 cut(s) 26
BseSI GKGCMC 2 cut(s) 1316, 1720
BseXI GCAGC 1 cut(s) 1001
BshFI GGCC 6 cut(s) 1080, 1198, 1462, 1565, 1691, 1749
BshNI GGYRCC 1 cut(s) 1717
BsiHKAI GWGCWC 3 cut(s) 207, 1316, 1702
BslFI GGGAC 2 cut(s) 70, 1593
BslI CCNNNNNNNGG 3 cut(s) 901, 1354, 1742
BsmAI GTCTC 3 cut(s) 217, 657, 1283
BsmBI CGTCTC 1 cut(s) 1283
BsmFI GGGAC 2 cut(s) 70, 1593
BsnI GGCC 6 cut(s) 1080, 1198, 1462, 1565, 1691, 1749
Bsp1286I GDGCHC 4 cut(s) 207, 1316, 1702, 1720
Bsp1407I TGTACA 2 cut(s) 1502, 1660
Bsp143I GATC 6 cut(s) 178, 351, 682, 1360, 1514, 1522
Bsp19I CCATGG 1 cut(s) 100
BspACI CCGC 3 cut(s) 437, 1446, 1680
BspANI GGCC 6 cut(s) 1080, 1198, 1462, 1565, 1691, 1749
BspCNI CTCAG 1 cut(s) 935
BspLI GGNNCC 5 cut(s) 58, 59, 240, 753, 1719
BspMI ACCTGC 4 cut(s) 113, 902, 1450, 1547
BspPI GGATC 5 cut(s) 173, 690, 1355, 1522, 1530
BspT107I GGYRCC 1 cut(s) 1717
BsrGI TGTACA 2 cut(s) 1502, 1660
BsrI ACTGG 3 cut(s) 35, 245, 260
BssECI CCNNGG 4 cut(s) 100, 1298, 1475, 1736
BssMI GATC 6 cut(s) 178, 351, 682, 1360, 1514, 1522
BssT1I CCWWGG 4 cut(s) 100, 1298, 1475, 1736
Bst4CI ACNGT 2 cut(s) 1104, 1507
Bst6I CTCTTC 3 cut(s) 882, 939, 1777
BstAUI TGTACA 2 cut(s) 1502, 1660
BstC8I GCNNGC 1 cut(s) 850
BstDEI CTNAG 4 cut(s) 476, 569, 922, 1173
BstDSI CCRYGG 1 cut(s) 100
BstF5I GGATG 3 cut(s) 421, 835, 1627
BstKTI GATC 6 cut(s) 181, 354, 685, 1363, 1517, 1525
BstMAI GTCTC 3 cut(s) 217, 657, 1283
BstMBI GATC 6 cut(s) 178, 351, 682, 1360, 1514, 1522
BstMWI GCNNNNNNNGC 9 cut(s) 172, 1454, 1468, 1562, 1688, 1697, 1706, 1715, 1766
BstNSI RCATGY 3 cut(s) 824, 866, 1835
BstPAI GACNNNNGTC 1 cut(s) 492
BstSLI GKGCMC 2 cut(s) 1316, 1720
BstV1I GCAGC 1 cut(s) 1001
BstV2I GAAGAC 1 cut(s) 717
BstX2I RGATCY 2 cut(s) 1360, 1514
BstYI RGATCY 2 cut(s) 1360, 1514
BsuRI GGCC 6 cut(s) 1080, 1198, 1462, 1565, 1691, 1749
BtgI CCRYGG 1 cut(s) 100
BtsCI GGATG 3 cut(s) 421, 835, 1627
BtsI GCAGTG 1 cut(s) 1137
BtsIMutI CAGTG 1 cut(s) 1137
BveI ACCTGC 4 cut(s) 113, 902, 1450, 1547
Cac8I GCNNGC 1 cut(s) 850
Cfr13I GGNCC 1 cut(s) 57
CseI GACGC 1 cut(s) 1300
Csp6I GTAC 9 cut(s) 28, 283, 734, 818, 1503, 1661, 1667, 1733, 1791
CviQI GTAC 9 cut(s) 28, 283, 734, 818, 1503, 1661, 1667, 1733, 1791
DdeI CTNAG 4 cut(s) 476, 569, 922, 1173
DpnI GATC 6 cut(s) 180, 353, 684, 1362, 1516, 1524
DpnII GATC 6 cut(s) 178, 351, 682, 1360, 1514, 1522
EaeI YGGCCR 1 cut(s) 1078
Eam1104I CTCTTC 3 cut(s) 882, 939, 1777
EarI CTCTTC 3 cut(s) 882, 939, 1777
Ecl136II GAGCTC 1 cut(s) 205
Eco130I CCWWGG 4 cut(s) 100, 1298, 1475, 1736
Eco147I AGGCCT 1 cut(s) 1198
Eco24I GRGCYC 1 cut(s) 207
Eco32I GATATC 1 cut(s) 1239
Eco47I GGWCC 1 cut(s) 57
Eco53kI GAGCTC 1 cut(s) 205
Eco57I CTGAAG 1 cut(s) 570
EcoICRI GAGCTC 1 cut(s) 205
EcoO109I RGGNCCY 1 cut(s) 57
EcoRV GATATC 1 cut(s) 1239
EcoT14I CCWWGG 4 cut(s) 100, 1298, 1475, 1736
EcoT38I GRGCYC 1 cut(s) 207
ErhI CCWWGG 4 cut(s) 100, 1298, 1475, 1736
Esp3I CGTCTC 1 cut(s) 1283
FalI AAGNNNNNCTT 2 cut(s) 1705, 1737
FaqI GGGAC 2 cut(s) 70, 1593
FbaI TGATCA 1 cut(s) 351
Fnu4HI GCNGC 3 cut(s) 990, 1446, 1680
FokI GGATG 3 cut(s) 428, 842, 1634
FriOI GRGCYC 1 cut(s) 207
Fsp4HI GCNGC 3 cut(s) 990, 1446, 1680
FspBI CTAG 8 cut(s) 89, 471, 770, 1025, 1043, 1476, 1482, 1737
GluI GCNGC 3 cut(s) 990, 1446, 1680
GsuI CTGGAG 3 cut(s) 52, 723, 1227
HaeIII GGCC 6 cut(s) 1080, 1198, 1462, 1565, 1691, 1749
HgaI GACGC 1 cut(s) 1300
HincII GTYRAC 3 cut(s) 487, 868, 1345
HindII GTYRAC 3 cut(s) 487, 868, 1345
HindIII AAGCTT 2 cut(s) 173, 1469
HinfI GANTC 5 cut(s) 14, 713, 971, 977, 1823
HpaI GTTAAC 1 cut(s) 1345
HphI GGTGA 4 cut(s) 164, 1408, 1624, 1716
Hpy166II GTNNAC 6 cut(s) 250, 487, 868, 1231, 1314, 1345
Hpy188I TCNGA 4 cut(s) 751, 925, 976, 982
Hpy188III TCNNGA 7 cut(s) 89, 182, 740, 1043, 1244, 1388, 1599
Hpy8I GTNNAC 6 cut(s) 250, 487, 868, 1231, 1314, 1345
HpyAV CCTTC 6 cut(s) 99, 406, 545, 756, 1555, 1731
HpyCH4III ACNGT 2 cut(s) 1104, 1507
HpyCH4IV ACGT 1 cut(s) 489
HpyF10VI GCNNNNNNNGC 9 cut(s) 172, 1454, 1468, 1562, 1688, 1697, 1706, 1715, 1766
HpyF3I CTNAG 4 cut(s) 476, 569, 922, 1173
HpySE526I ACGT 1 cut(s) 489
KflI GGGWCCC 1 cut(s) 57
Ksp22I TGATCA 1 cut(s) 351
KspAI GTTAAC 1 cut(s) 1345
Kzo9I GATC 6 cut(s) 178, 351, 682, 1360, 1514, 1522
LmnI GCTCC 1 cut(s) 202
Lsp1109I GCAGC 1 cut(s) 1001
LweI GCATC 4 cut(s) 109, 835, 1718, 1811
MaeI CTAG 8 cut(s) 89, 471, 770, 1025, 1043, 1476, 1482, 1737
MaeII ACGT 1 cut(s) 489
MaeIII GTNAC 3 cut(s) 914, 995, 1489
MalI GATC 6 cut(s) 180, 353, 684, 1362, 1516, 1524
MboI GATC 6 cut(s) 178, 351, 682, 1360, 1514, 1522
MfeI CAATTG 1 cut(s) 542
MflI RGATCY 2 cut(s) 1360, 1514
MhlI GDGCHC 4 cut(s) 207, 1316, 1702, 1720
MlsI TGGCCA 1 cut(s) 1080
MluNI TGGCCA 1 cut(s) 1080
MlyI GAGTC 2 cut(s) 23, 707
MmeI TCCRAC 4 cut(s) 180, 1167, 1201, 1612
Mox20I TGGCCA 1 cut(s) 1080
MscI TGGCCA 1 cut(s) 1080
MseI TTAA 7 cut(s) 324, 855, 1020, 1257, 1305, 1344, 1863
MslI CAYNNNNRTG 2 cut(s) 516, 933
Msp20I TGGCCA 1 cut(s) 1080
MspA1I CMGCKG 1 cut(s) 20
MunI CAATTG 1 cut(s) 542
MwoI GCNNNNNNNGC 9 cut(s) 172, 1454, 1468, 1562, 1688, 1697, 1706, 1715, 1766
NcoI CCATGG 1 cut(s) 100
NdeII GATC 6 cut(s) 178, 351, 682, 1360, 1514, 1522
NlaIV GGNNCC 5 cut(s) 58, 59, 240, 753, 1719
NmuCI GTSAC 1 cut(s) 1489
NspI RCATGY 3 cut(s) 824, 866, 1835
PaqCI CACCTGC 2 cut(s) 113, 1450
PceI AGGCCT 1 cut(s) 1198
PciI ACATGT 2 cut(s) 820, 862
PfeI GAWTC 3 cut(s) 971, 977, 1823
PflMI CCANNNNNTGG 1 cut(s) 901
PkrI GCNGC 3 cut(s) 991, 1447, 1681
PleI GAGTC 2 cut(s) 22, 707
PpsI GAGTC 2 cut(s) 22, 707
PpuMI RGGWCCY 1 cut(s) 57
PscI ACATGT 2 cut(s) 820, 862
PshAI GACNNNNGTC 1 cut(s) 492
Psp124BI GAGCTC 1 cut(s) 207
Psp5II RGGWCCY 1 cut(s) 57
PspN4I GGNNCC 5 cut(s) 58, 59, 240, 753, 1719
PspPI GGNCC 1 cut(s) 57
PspPPI RGGWCCY 1 cut(s) 57
PsuI RGATCY 2 cut(s) 1360, 1514
PvuII CAGCTG 1 cut(s) 20
RsaI GTAC 9 cut(s) 29, 284, 735, 819, 1504, 1662, 1668, 1734, 1792
RsaNI GTAC 9 cut(s) 28, 283, 734, 818, 1503, 1661, 1667, 1733, 1791
RseI CAYNNNNRTG 2 cut(s) 516, 933
SacI GAGCTC 1 cut(s) 207
SaqAI TTAA 7 cut(s) 324, 855, 1020, 1257, 1305, 1344, 1863
SatI GCNGC 3 cut(s) 990, 1446, 1680
Sau3AI GATC 6 cut(s) 178, 351, 682, 1360, 1514, 1522
Sau96I GGNCC 1 cut(s) 57
ScaI AGTACT 2 cut(s) 29, 1792
SchI GAGTC 2 cut(s) 23, 707
SduI GDGCHC 4 cut(s) 207, 1316, 1702, 1720
SfaNI GCATC 4 cut(s) 109, 835, 1718, 1811
SinI GGWCC 1 cut(s) 57
SmiMI CAYNNNNRTG 2 cut(s) 516, 933
SmlI CTYRAG 2 cut(s) 667, 1812
SmoI CTYRAG 2 cut(s) 667, 1812
SseBI AGGCCT 1 cut(s) 1198
SsiI CCGC 3 cut(s) 437, 1446, 1680
SspMI CTAG 8 cut(s) 89, 471, 770, 1025, 1043, 1476, 1482, 1737
SstI GAGCTC 1 cut(s) 207
StuI AGGCCT 1 cut(s) 1198
StyI CCWWGG 4 cut(s) 100, 1298, 1475, 1736
TaaI ACNGT 2 cut(s) 1104, 1507
TaiI ACGT 1 cut(s) 492
TatI WGTACW 6 cut(s) 27, 817, 1502, 1660, 1666, 1790
TauI GCSGC 2 cut(s) 1448, 1682
TfiI GAWTC 3 cut(s) 971, 977, 1823
Tru1I TTAA 7 cut(s) 324, 855, 1020, 1257, 1305, 1344, 1863
Tru9I TTAA 7 cut(s) 324, 855, 1020, 1257, 1305, 1344, 1863
TscAI CASTG 1 cut(s) 1137
TseFI GTSAC 1 cut(s) 1489
TseI GCWGC 1 cut(s) 989
Tsp45I GTSAC 1 cut(s) 1489
TspRI CASTG 1 cut(s) 1137
Van91I CCANNNNNTGG 1 cut(s) 901
VneI GTGCAC 1 cut(s) 1312
VpaK11BI GGWCC 1 cut(s) 57
XapI RAATTY 5 cut(s) 41, 65, 698, 1067, 1147
XbaI TCTAGA 2 cut(s) 88, 1042
XceI RCATGY 3 cut(s) 824, 866, 1835
XcmI CCANNNNNNNNNTGG 2 cut(s) 252, 276
XmaJI CCTAGG 2 cut(s) 1475, 1736
XspI CTAG 8 cut(s) 89, 471, 770, 1025, 1043, 1476, 1482, 1737
ZrmI AGTACT 2 cut(s) 29, 1792
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.