Rorug07G0294600

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0000282
Physical Location & Seq
Forward (+)
28828642 .. 28834735
6094 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0294600.1

Sequence Viewer

Length: 3351 bp
ATGGAAGAGAGCAAACATAAATCTAGGAAGGAAGGGGAAGCTAAGAGGAGTCATCGAGATCGGGATCGGGATCGTGAGAAGGAGAAGAAAGTAGATAGACACAGAGAAAAAGATAGAGGAGATAAGAGTCGTGACAGCCGACGATATGAGCGAGAAAAGAGTTCTGATTCTGAGGAGAGGTACGTGAGAGAGAAGCATCGAGATAAGGAGGTTGATAGCATACGAAGCAGAGATGATGAACGAGAGAGAAGCAAAGATAGGAAAAGGGATAAAGATAAAGAGAGGGACAAGGAGAGGGAGAGAGTCAAAGAAAAGGAGAAGAGGGAGCGGAAGGAGAGGGAGAGAAGGGAACGTGAAAGGGAGGCAGAAGAGAGGGCGAGGGAGAAGAGGGAGAGGGAAAAGGAAAAAGATCGAGAGAGGAGGATAAGGGAGAGGGAGAGGCATAGGGATGTTGATACTGATGATTCTGAGGATGATCTTAGGGAGCGTGGTAGGAAGCGGCATAAGAAGAGTGATAGCGAGTACAAGGAGAGAGATCGGGAAAGGAGTATTAGTAGGTCAAACAGACATAGGGATGATGGAATTGAGAGCCCAAGAAAGAGGAGTGTTGAAGATGATTCAGATAAAAAGGAGAAGCCAACCCGTGAGGAGGAGTTAGAGGACGAACAACGGAAATTGGATGAGGAAATGGAAAAGCGGAGGAGGAGAGTTCAGGAGTGGCAAGAGTTAAAGAGAAAGAAGGAAGAAGATGAGAGAGAGAAGCGTGGGGAAGATAATGTTGATGACACCAAGTCTGGAAAGACCTGGACTCTTGATGGAGAATCTGATGACGAAGAAGCAGGCAAATCAGAGACAGGTATGGACATTGATAGGGAGGTCAATGGGGATGATCTTACTGATGGAGAAGTTGTAGATGCCATCGTGCCAGATATTAATACAACAGCTGCTGCAGGGAATGAGGAAGTTGATCCATTAGAAGCTTTTATGCAATCTGTGGTGATGCCCGAAGTTGCAAAGCTGAATGCTACTGTGGAACCCTCAATTGTTGATGACAAGAACAAGGAGAAAAAAGTTGATACAAGTAATGGTGAAAAGCTGAGGGCAGGTTCAAATAAATCTATTGGCAGAATAATTCCTGGGGAGGATTCTGACTCGGATTACGGGGACCTTGAGAATGATGACGATCCTTTAGAAAATGAAGATGATGATGAGTTCATAAAAAGGGTGAAAAAGACGAAAGCTGAGAAACTTTCCTTAGTTGACCACTCGAAGATAGATTATATGCCATTCCGTAAAAAATTTTATATCGAGGTGAAGGAGATCTCAAGGATGACGCCAGATGAGGTTGGTGCATACCGGAAACAATTGGAATTGAAGATACATGGTAAGGATGTGCCAAAGCCCATAAAGAGCTGGCACCAAACTGGACTTACAAGTAAAATCTTGGATATAATAAAGAAGCTCAACTATGAAAAGCCAATGCCAATTCAAGCTCAGGCACTGCCCATAATTATGAGTGGTCGAGACTGCATTGGCGTTGGAAAAACTGGATCAGGCAAAACCCTTGCATTTGTACTGCCAATGCTGAGGCATATCAAGGACCAGCCACCTGTGGTAGCTGGGGATGGGCCTATTGGGCTTATTATGGCACCAACCAGGGAGCTTGTTCAGCAGATTCACAGTGATATCAAAAAGTTTGCCAAGGTTCTGGGTCTCAGGTGTGTGCCTGTATACGGAGGTTCAGGTGTTGCACAACAAATTAGTGAACTGAAGCGGGGTGCTGAAATTGTTGTCTGTACTCCTGGTAGGATGATTGACATACTTTGCACAAGTGGAGGAAAAATTACAAATCTGCGTAGAGTAACTTATTTGGTCATGGATGAAGCTGATCGAATGTTTGACATGGGTTTTGAACCTCAAATTACTCGAATTGTTCAAAATATTCGTCCAGATCGCCAAACCGTATTATTTTCTGCCACTTTCCCTCGGCAGGTTGAAGTTTTGGCTCGTAAAGTCTTAGATAAACCTGTGGAAATACAAGTTGGCGGAAGGAGTGTTGTGAATAAGGACATCATGCAATTGGTCGAAGTGAGGCAGGAACATGAAAGGTTCTTGAGATTGTTAGAGTTACTTGGAGAATGGTATGAAAAAGGAAAAATTTTGATATTTGTACAATCGCAGGACAAATGTGATGCTTTATTTAGAGATCTGCTTAAGCATGGATATCCTTGTCTCTCACTTCATGGGGCCAAAGATCAAACAGATCGTGAGTCCACCATAGCTGATTTTAAGAGCAATGTTTGTAATTTGTTGATTGCAACCAGTGTTGCTGCTAGAGGGCTAGATGTCAAGGAGCTTGAACTGGTGATAAATTTTGATGTCCCAAATCACTATGAGGACTATGTTCACCGTGTTGGACGTACTGGCCGTGCTGGCCGGAAAGGCTGTGCTATAACTTTTGTCTCTGAGGAAGATGCTAGATATGCTCCAGATCTTGTGAAAGCATTGGAACTTTCTGAGCAAGTTGTTCCAGATGACTTGAAATCTCTTGCTGATGATTTTATGTCAAAAGTAAATCAGGGACTTGCACAAGCCCATGGGACTGGTTATGGGGGAAGTGGCTTTAAATTTAATGTAGAGGAAGATGAAGTAAGGAGAGCGGCAAAGAAAGCACAGGCCAAAGAATATGGTTTTGAGGAAGACAAGTCAGATTCAGAAGATGAAGATGAAGGTATTCGAAAAGCAGGGGGTGATATCTCTCAGCAGGCTGCTCTTGCTCAGATAGCTGCTATTGCTGCTGCCTCCAAAGCTACTACAGCAATGCAGACTCCTGCTTCTGCTGCCCAACTGCTTCCCAACGGTGGACTGCCTGTCGCTCTTCCAGGTGTCGGTGGTGTAGGTGCATTACCAGGAACAGCAGCAGTTGTGCCTGGGACTGGTCTGCCTCTTGTTGGCAGTGATGGGGCAGCTCGGGCTGCAGCACTTGCGGCTGCCATGAACTTACAGCATAACCTGGCAAAGATCCACGCTGATGCAATGCCTGAACACTATGAAGCAGAGTTGGAGATAAATGATTTTCCCCAGAATGCTAGATGGAAAGTCACCCACAAGGAAACGTTGGGCCCTATATCAGAGTGGACTGGAGCTGCGATTACTACCAGGGGTCAGTATTTCCCACCAGGGAAGGTTCCAGGACCAGGAGATCGCAAGCTCTACTTGTTTATTGAGGGCCCTACCGAACAGTCTGTCAAGAGAGCTAAAGCAGAATTAAAAGGTGTTTTGGAAGAGATCTCAAATCAGGCATTATCACTCCCTGGAGGAAATCAGCAAGGCAGATATCAAGTTATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2 PPR_3

Protein Analysis

1116

Amino Acids

126.53

Weight (kDa)

6.01

Isoelectric Point (pI)

41.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 495 - 666 1.6e-49 DEAD/DEAH box helicase
Helicase_C PF00271 706 - 812 2e-29 Helicase conserved C-terminal domain
KH_12 PF23469 1018 - 1094 4.6e-14 KH-domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000282)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g12350 FvH4_5g12380 FvH4_5g12392 FvH4_5g12400 FvH4_5g12430
malus_domestica MD06G1213300.v1.1 MD06G1213500.v1.1 MD06G1213700.v1.1 MD06G1213800.v1.1 MD06G1213900.v1.1 MD06G1214000.v1.1 MD06G1214400.v1.1 MD14G1224300.v1.1 MD14G1224400.v1.1 MD14G1224600.v1.1 MD14G1224700.v1.1 MD14G1224800.v1.1 MD14G1224900.v1.1 MD14G1225100.v1.1 MD14G1225400.v1.1
prunus_persica Prupe.5G219700_v2.0.a1 Prupe.5G219800_v2.0.a1 Prupe.5G219900_v2.0.a1 Prupe.8G078500_v2.0.a1
pyrus_communis pycom06g19100 pycom14g18590 pycom14g18600 pycom14g18610 pycom14g18660
rosa_chinensis RchiOBHm_Chr0c12g0499591 RchiOBHm_Chr2g0140591 RchiOBHm_Chr2g0140631 RchiOBHm_Chr2g0140641 RchiOBHm_Chr2g0140651 RchiOBHm_Chr4g0417991 RchiOBHm_Chr4g0418001 RchiOBHm_Chr4g0418101 RchiOBHm_Chr4g0418111 RchiOBHm_Chr4g0418121 RchiOBHm_Chr6g0280191 RchiOBHm_Chr6g0280241 RchiOBHm_Chr6g0280251 RchiOBHm_Chr7g0185031 RchiOBHm_Chr7g0185051 RchiOBHm_Chr7g0185081 RchiOBHm_Chr7g0185091 RchiOBHm_Chr7g0185111 RchiOBHm_Chr7g0185131 RchiOBHm_Chr7g0185151 RchiOBHm_Chr7g0185161 RchiOBHm_Chr7g0185191 RchiOBHm_Chr7g0222431
rosa_laevigata RLG00000002061 RLG00000004934 RLG00000004935 RLG00000004937 RLG00000013071 RLG00000013077 RLG00000019621 RLG00000019881 RLG00000019884 RLG00000019887 RLG00000019888 RLG00000028839
rosa_multiflora Rmu_co8014370.1_g000001 Rmu_co8334683.1_g000001 Rmu_co8440383.1_g000001 Rmu_sc0002826.1_g000008 Rmu_sc0002826.1_g000009 Rmu_sc0002826.1_g000030 Rmu_sc0004483.1_g000005 Rmu_sc0004638.1_g000003 Rmu_sc0004638.1_g000004 Rmu_sc0005756.1_g000003 Rmu_sc0006493.1_g000023 Rmu_sc0006493.1_g000024 Rmu_sc0007137.1_g000017 Rmu_sc0008496.1_g000022 Rmu_sc0008769.1_g000003 Rmu_sc0013612.1_g000026 Rmu_sc0013864.1_g000001 Rmu_sc0013864.1_g000007 Rmu_sc0013864.1_g000008 Rmu_sc0030128.1_g000001 Rmu_sc0041660.1_g000001 Rmu_ssc0000289.1_g000001
rosa_roxburghii Rroxscaffold_2G00104640 Rroxscaffold_3G00253970 Rroxscaffold_3G00269170 Rroxscaffold_3G00269200 Rroxscaffold_3G00269210 Rroxscaffold_3G00269220 Rroxscaffold_3G00269230 Rroxscaffold_7G00188320
rosa_rugosa Rorug01G0052300 Rorug05G0212000 Rorug06G0130700 Rorug06G0131100 Rorug06G0466200 Rorug06G0466300 Rorug06G0466400 Rorug06G0466500 Rorug06G0466700 Rorug07G0208500 Rorug07G0294600
rosa_samantha Rh4AG137600 Rh6DG237300 Rh6DG237800 Rh7AG076200 Rh7AG076300 Rh7AG076500 Rh7AG076600 Rh7AG350700 Rh7BG070800 Rh7BG070900 Rh7BG071000 Rh7BG071100
rosa_wichuraiana Rw6G020990 Rw6G021010 Rw7G005900 Rw7G005910 Rw7G005920 Rw7G005940 Rw7G005950 Rw7G005970 Rw7G029600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 1094, 1981
AccB1I GGYRCC 2 cut(s) 1416, 1648
AccBSI CCGCTC 2 cut(s) 328, 2660
AccI GTMKAC 1 cut(s) 1731
AciI CCGC 7 cut(s) 328, 499, 697, 1774, 2046, 2660, 2987
AclI AACGTT 1 cut(s) 3116
AclWI GGATC 6 cut(s) 72, 78, 962, 1178, 1558, 3016
AcoI YGGCCR 2 cut(s) 2425, 2434
AcsI RAATTY 4 cut(s) 1298, 2157, 2371, 2627
AcuI CTGAAG 1 cut(s) 1790
AcyI GRCGYC 1 cut(s) 1334
AfaI GTAC 6 cut(s) 182, 524, 1575, 1798, 2172, 2422
AfiI CCNNNNNNNGG 8 cut(s) 649, 1733, 1990, 2861, 2888, 2936, 2951, 3197
AflII CTTAAG 1 cut(s) 2213
AgsI TTSAA 9 cut(s) 611, 1110, 1375, 1490, 1913, 1937, 1997, 2360, 2542
AhdI GACNNNNNGTC 2 cut(s) 790, 2870
AjuI GAANNNNNNNTTGG 2 cut(s) 2025, 2057
Alw26I GTCTC 5 cut(s) 845, 1518, 1718, 2237, 2467
AlwI GGATC 6 cut(s) 72, 78, 962, 1178, 1558, 3016
AlwNI CAGNNNCTG 2 cut(s) 947, 1501
Ama87I CYCGRG 1 cut(s) 2970
AoxI GGCC 7 cut(s) 1628, 2247, 2425, 2434, 2676, 3121, 3229
ApaI GGGCCC 2 cut(s) 3125, 3233
ApoI RAATTY 4 cut(s) 1298, 2157, 2371, 2627
ArsI GACNNNNNNTTYG 6 cut(s) 1892, 1924, 1930, 1962, 3231, 3263
AseI ATTAAT 1 cut(s) 933
Asp700I GAANNNNTTC 1 cut(s) 2733
AspS9I GGNCC 9 cut(s) 1165, 1600, 1628, 2247, 3121, 3122, 3194, 3229, 3230
AsuHPI GGTGA 8 cut(s) 1009, 1100, 1237, 1324, 2377, 2399, 2762, 3094
AsuII TTCGAA 1 cut(s) 2737
AvaI CYCGRG 1 cut(s) 2970
AvaII GGWCC 3 cut(s) 1165, 1600, 3194
BaeGI GKGCMC 2 cut(s) 3125, 3233
BanI GGYRCC 2 cut(s) 1416, 1648
BanII GRGCYC 3 cut(s) 593, 3125, 3233
BbsI GAAGAC 1 cut(s) 2706
BbvCI CCTCAGC 2 cut(s) 1097, 1586
BccI CCATC 7 cut(s) 572, 809, 893, 926, 1619, 2954, 3087
BceAI ACGGC 1 cut(s) 2412
BcoDI GTCTC 5 cut(s) 845, 1518, 1718, 2237, 2467
BfaI CTAG 5 cut(s) 24, 2334, 2342, 2478, 3090
BfmI CTRYAG 3 cut(s) 948, 2814, 2976
BfrI CTTAAG 1 cut(s) 2213
BfuAI ACCTGC 2 cut(s) 1094, 1981
BglI GCCNNNNNGGC 3 cut(s) 1636, 2433, 2442
BglII AGATCT 4 cut(s) 1320, 2206, 2491, 3288
Bme18I GGWCC 3 cut(s) 1165, 1600, 3194
BmeRI GACNNNNNGTC 2 cut(s) 790, 2870
BmeT110I CYCGRG 1 cut(s) 2970
BmgT120I GGNCC 9 cut(s) 1165, 1600, 1628, 2247, 3121, 3122, 3194, 3229, 3230
BmiI GGNNCC 8 cut(s) 1035, 1166, 1418, 1650, 2248, 3123, 3189, 3231
BmsI GCATC 6 cut(s) 205, 904, 990, 2182, 2464, 3022
BpiI GAAGAC 1 cut(s) 2706
BpmI CTGGAG 3 cut(s) 2472, 3162, 3336
Bpu10I CCTNAGC 3 cut(s) 1097, 1494, 1586
Bpu14I TTCGAA 1 cut(s) 2737
BpuEI CTTGAG 3 cut(s) 1190, 1309, 2134
BsaAI YACGTR 1 cut(s) 184
BsaBI GATNNNNATC 3 cut(s) 63, 69, 1182
BsaHI GRCGYC 1 cut(s) 1334
BsaI GGTCTC 1 cut(s) 1718
BsaJI CCNNGG 9 cut(s) 1136, 1656, 1701, 1985, 2596, 2930, 3159, 3179, 3313
BsaWI WCCGGW 1 cut(s) 1356
BsaXI ACNNNNNCTCC 4 cut(s) 538, 568, 894, 924
Bsc4I CCNNNNNNNGG 8 cut(s) 649, 1733, 1990, 2861, 2888, 2936, 2951, 3197
Bse1I ACTGG 8 cut(s) 1429, 1552, 2322, 2367, 2428, 2608, 2941, 3145
Bse3DI GCAATG 3 cut(s) 2302, 2826, 3042
Bse8I GATNNNNATC 3 cut(s) 63, 69, 1182
BseDI CCNNGG 9 cut(s) 1136, 1656, 1701, 1985, 2596, 2930, 3159, 3179, 3313
BseJI GATNNNNATC 3 cut(s) 63, 69, 1182
BseLI CCNNNNNNNGG 8 cut(s) 649, 1733, 1990, 2861, 2888, 2936, 2951, 3197
BseMI GCAATG 3 cut(s) 2302, 2826, 3042
BseNI ACTGG 8 cut(s) 1429, 1552, 2322, 2367, 2428, 2608, 2941, 3145
BseRI GAGGAG 9 cut(s) 61, 132, 188, 433, 616, 662, 665, 715, 718
BseSI GKGCMC 2 cut(s) 3125, 3233
BseYI CCCAGC 1 cut(s) 1619
BshFI GGCC 7 cut(s) 1630, 2249, 2427, 2436, 2678, 3123, 3231
BshNI GGYRCC 2 cut(s) 1416, 1648
BsiHKCI CYCGRG 1 cut(s) 2970
BsiSI CCGG 2 cut(s) 1357, 2437
BslFI GGGAC 6 cut(s) 299, 1178, 2366, 2595, 2614, 2947
BslI CCNNNNNNNGG 8 cut(s) 649, 1733, 1990, 2861, 2888, 2936, 2951, 3197
BsmAI GTCTC 5 cut(s) 845, 1518, 1718, 2237, 2467
BsmFI GGGAC 6 cut(s) 299, 1178, 2366, 2595, 2614, 2947
BsmI GAATGC 2 cut(s) 1027, 3091
BsnI GGCC 7 cut(s) 1630, 2249, 2427, 2436, 2678, 3123, 3231
Bso31I GGTCTC 1 cut(s) 1718
BsoBI CYCGRG 1 cut(s) 2970
Bsp119I TTCGAA 1 cut(s) 2737
Bsp120I GGGCCC 2 cut(s) 3121, 3229
Bsp1286I GDGCHC 3 cut(s) 593, 3125, 3233
Bsp1407I TGTACA 1 cut(s) 2170
Bsp19I CCATGG 1 cut(s) 2596
BspACI CCGC 7 cut(s) 328, 499, 697, 1774, 2046, 2660, 2987
BspANI GGCC 7 cut(s) 1630, 2249, 2427, 2436, 2678, 3123, 3231
BspLI GGNNCC 8 cut(s) 1035, 1166, 1418, 1650, 2248, 3123, 3189, 3231
BspMAI CTGCAG 2 cut(s) 952, 2980
BspMI ACCTGC 2 cut(s) 1094, 1981
BspPI GGATC 6 cut(s) 72, 78, 962, 1178, 1558, 3016
BspQI GCTCTTC 1 cut(s) 2883
BspT104I TTCGAA 1 cut(s) 2737
BspT107I GGYRCC 2 cut(s) 1416, 1648
BspTI CTTAAG 1 cut(s) 2213
BspTNI GGTCTC 1 cut(s) 1718
BsrBI CCGCTC 2 cut(s) 328, 2660
BsrDI GCAATG 3 cut(s) 2302, 2826, 3042
BsrGI TGTACA 1 cut(s) 2170
BsrI ACTGG 8 cut(s) 1429, 1552, 2322, 2367, 2428, 2608, 2941, 3145
BssECI CCNNGG 9 cut(s) 1136, 1656, 1701, 1985, 2596, 2930, 3159, 3179, 3313
BssNAI GTATAC 1 cut(s) 1732
BssNI GRCGYC 1 cut(s) 1334
BssT1I CCWWGG 2 cut(s) 1701, 2596
Bst1107I GTATAC 1 cut(s) 1732
Bst4CI ACNGT 6 cut(s) 1030, 1682, 1963, 2411, 2861, 3243
Bst6I CTCTTC 6 cut(s) 314, 363, 380, 503, 2883, 3279
BstACI GRCGYC 1 cut(s) 1334
BstAFI CTTAAG 1 cut(s) 2213
BstAPI GCANNNNNTGC 1 cut(s) 2984
BstAUI TGTACA 1 cut(s) 2170
BstBAI YACGTR 1 cut(s) 184
BstBI TTCGAA 1 cut(s) 2737
BstC8I GCNNGC 5 cut(s) 841, 1415, 2434, 2766, 3209
BstDSI CCRYGG 1 cut(s) 2596
BstMAI GTCTC 5 cut(s) 845, 1518, 1718, 2237, 2467
BstSFI CTRYAG 3 cut(s) 948, 2814, 2976
BstSLI GKGCMC 2 cut(s) 3125, 3233
BstV2I GAAGAC 1 cut(s) 2706
BstX2I RGATCY 5 cut(s) 1320, 2206, 2491, 3021, 3288
BstXI CCANNNNNNTGG 2 cut(s) 1708, 2603
BstYI RGATCY 5 cut(s) 1320, 2206, 2491, 3021, 3288
BstZ17I GTATAC 1 cut(s) 1732
BsuRI GGCC 7 cut(s) 1630, 2249, 2427, 2436, 2678, 3123, 3231
BtgI CCRYGG 1 cut(s) 2596
BtsI GCAGTG 2 cut(s) 1499, 2962
BtsIMutI CAGTG 4 cut(s) 1499, 1687, 2329, 2962
BveI ACCTGC 2 cut(s) 1094, 1981
Cac8I GCNNGC 5 cut(s) 841, 1415, 2434, 2766, 3209
CaiI CAGNNNCTG 2 cut(s) 947, 1501
Cfr13I GGNCC 9 cut(s) 1165, 1600, 1628, 2247, 3121, 3122, 3194, 3229, 3230
CseI GACGC 1 cut(s) 1342
Csp6I GTAC 6 cut(s) 181, 523, 1574, 1797, 2171, 2421
CviAII CATG 9 cut(s) 1382, 1876, 1903, 2074, 2102, 2219, 2243, 2597, 2995
CviQI GTAC 6 cut(s) 181, 523, 1574, 1797, 2171, 2421
DraI TTTAAA 1 cut(s) 2626
DriI GACNNNNNGTC 2 cut(s) 790, 2870
EaeI YGGCCR 2 cut(s) 2425, 2434
Eam1104I CTCTTC 6 cut(s) 314, 363, 380, 503, 2883, 3279
Eam1105I GACNNNNNGTC 2 cut(s) 790, 2870
EarI CTCTTC 6 cut(s) 314, 363, 380, 503, 2883, 3279
EciI GGCGGA 1 cut(s) 2061
Eco130I CCWWGG 2 cut(s) 1701, 2596
Eco24I GRGCYC 3 cut(s) 593, 3125, 3233
Eco31I GGTCTC 1 cut(s) 1718
Eco32I GATATC 4 cut(s) 1687, 2225, 2755, 3338
Eco47I GGWCC 3 cut(s) 1165, 1600, 3194
Eco57I CTGAAG 1 cut(s) 1790
Eco88I CYCGRG 1 cut(s) 2970
EcoO109I RGGNCCY 4 cut(s) 1165, 3122, 3229, 3230
EcoRV GATATC 4 cut(s) 1687, 2225, 2755, 3338
EcoT14I CCWWGG 2 cut(s) 1701, 2596
EcoT38I GRGCYC 3 cut(s) 593, 3125, 3233
ErhI CCWWGG 2 cut(s) 1701, 2596
FaeI CATG 9 cut(s) 1385, 1879, 1906, 2077, 2105, 2222, 2246, 2600, 2998
FalI AAGNNNNNCTT 2 cut(s) 3200, 3232
FaqI GGGAC 6 cut(s) 299, 1178, 2366, 2595, 2614, 2947
FatI CATG 9 cut(s) 1381, 1875, 1902, 2073, 2101, 2218, 2242, 2596, 2994
FauI CCCGC 1 cut(s) 1767
FblI GTMKAC 1 cut(s) 1731
FriOI GRGCYC 3 cut(s) 593, 3125, 3233
FspBI CTAG 5 cut(s) 24, 2334, 2342, 2478, 3090
GsaI CCCAGC 1 cut(s) 1623
GsuI CTGGAG 3 cut(s) 2472, 3162, 3336
HaeIII GGCC 7 cut(s) 1630, 2249, 2427, 2436, 2678, 3123, 3231
HapII CCGG 2 cut(s) 1357, 2437
HgaI GACGC 1 cut(s) 1342
Hin1I GRCGYC 1 cut(s) 1334
Hin1II CATG 9 cut(s) 1385, 1879, 1906, 2077, 2105, 2222, 2246, 2600, 2998
HincII GTYRAC 1 cut(s) 1261
HindII GTYRAC 1 cut(s) 1261
HindIII AAGCTT 1 cut(s) 978
HpaII CCGG 2 cut(s) 1357, 2437
HphI GGTGA 8 cut(s) 1009, 1100, 1237, 1324, 2377, 2399, 2762, 3094
Hpy166II GTNNAC 7 cut(s) 1261, 1732, 1766, 2274, 2407, 2864, 3138
Hpy8I GTNNAC 7 cut(s) 1261, 1732, 1766, 2274, 2407, 2864, 3138
Hpy99I CGWCG 1 cut(s) 144
HpyCH4III ACNGT 6 cut(s) 1030, 1682, 1963, 2411, 2861, 3243
HpyCH4IV ACGT 4 cut(s) 183, 352, 2419, 3116
HpySE526I ACGT 4 cut(s) 183, 352, 2419, 3116
Hsp92I GRCGYC 1 cut(s) 1334
Hsp92II CATG 9 cut(s) 1385, 1879, 1906, 2077, 2105, 2222, 2246, 2600, 2998
LguI GCTCTTC 1 cut(s) 2883
LmnI GCTCC 6 cut(s) 325, 484, 1660, 2353, 2491, 3143
LweI GCATC 6 cut(s) 205, 904, 990, 2182, 2464, 3022
MaeI CTAG 5 cut(s) 24, 2334, 2342, 2478, 3090
MaeII ACGT 4 cut(s) 183, 352, 2419, 3116
MaeIII GTNAC 4 cut(s) 131, 1861, 2127, 3100
MbiI CCGCTC 2 cut(s) 328, 2660
MfeI CAATTG 3 cut(s) 1041, 1364, 2078
MflI RGATCY 5 cut(s) 1320, 2206, 2491, 3021, 3288
MhlI GDGCHC 3 cut(s) 593, 3125, 3233
MlyI GAGTC 7 cut(s) 58, 136, 312, 802, 1145, 2279, 2821
MmeI TCCRAC 3 cut(s) 1519, 2395, 3042
MroXI GAANNNNTTC 1 cut(s) 2733
MseI TTAA 7 cut(s) 728, 933, 2214, 2289, 2625, 2631, 3269
MslI CAYNNNNRTG 4 cut(s) 447, 573, 1511, 3030
MspA1I CMGCKG 1 cut(s) 944
MspCI CTTAAG 1 cut(s) 2213
MspI CCGG 2 cut(s) 1357, 2437
MunI CAATTG 3 cut(s) 1041, 1364, 2078
Mva1269I GAATGC 2 cut(s) 1027, 3091
NcoI CCATGG 1 cut(s) 2596
NlaIII CATG 9 cut(s) 1385, 1879, 1906, 2077, 2105, 2222, 2246, 2600, 2998
NlaIV GGNNCC 8 cut(s) 1035, 1166, 1418, 1650, 2248, 3123, 3189, 3231
NmeAIII GCCGAG 1 cut(s) 1966
NmuCI GTSAC 2 cut(s) 131, 3100
NspV TTCGAA 1 cut(s) 2737
PciSI GCTCTTC 1 cut(s) 2883
PcsI WCGNNNNNNNCGW 3 cut(s) 136, 148, 2425
PctI GAATGC 2 cut(s) 1027, 3091
PdmI GAANNNNTTC 1 cut(s) 2733
PfeI GAWTC 7 cut(s) 167, 464, 617, 821, 1145, 1675, 2711
PfoI TCCNGGA 1 cut(s) 3190
PleI GAGTC 7 cut(s) 57, 135, 311, 802, 1145, 2278, 2821
PpsI GAGTC 7 cut(s) 57, 135, 311, 802, 1145, 2278, 2821
Ppu21I YACGTR 1 cut(s) 184
PpuMI RGGWCCY 1 cut(s) 1165
PshBI ATTAAT 1 cut(s) 933
Psp1406I AACGTT 1 cut(s) 3116
Psp5II RGGWCCY 1 cut(s) 1165
PspFI CCCAGC 1 cut(s) 1619
PspN4I GGNNCC 8 cut(s) 1035, 1166, 1418, 1650, 2248, 3123, 3189, 3231
PspOMI GGGCCC 2 cut(s) 3121, 3229
PspPI GGNCC 9 cut(s) 1165, 1600, 1628, 2247, 3121, 3122, 3194, 3229, 3230
PspPPI RGGWCCY 1 cut(s) 1165
PstI CTGCAG 2 cut(s) 952, 2980
PstNI CAGNNNCTG 2 cut(s) 947, 1501
PsuI RGATCY 5 cut(s) 1320, 2206, 2491, 3021, 3288
PvuII CAGCTG 1 cut(s) 944
RsaI GTAC 6 cut(s) 182, 524, 1575, 1798, 2172, 2422
RsaNI GTAC 6 cut(s) 181, 523, 1574, 1797, 2171, 2421
RseI CAYNNNNRTG 4 cut(s) 447, 573, 1511, 3030
SapI GCTCTTC 1 cut(s) 2883
SaqAI TTAA 7 cut(s) 728, 933, 2214, 2289, 2625, 2631, 3269
Sau96I GGNCC 9 cut(s) 1165, 1600, 1628, 2247, 3121, 3122, 3194, 3229, 3230
SchI GAGTC 7 cut(s) 58, 136, 312, 802, 1145, 2279, 2821
SduI GDGCHC 3 cut(s) 593, 3125, 3233
SfaNI GCATC 6 cut(s) 205, 904, 990, 2182, 2464, 3022
SfcI CTRYAG 3 cut(s) 948, 2814, 2976
SfiI GGCCNNNNNGGCC 1 cut(s) 2433
SfuI TTCGAA 1 cut(s) 2737
SinI GGWCC 3 cut(s) 1165, 1600, 3194
SmiMI CAYNNNNRTG 4 cut(s) 447, 573, 1511, 3030
SmlI CTYRAG 4 cut(s) 1169, 1324, 2113, 2213
SmoI CTYRAG 4 cut(s) 1169, 1324, 2113, 2213
SsiI CCGC 7 cut(s) 328, 499, 697, 1774, 2046, 2660, 2987
SspI AATATT 1 cut(s) 1942
SspMI CTAG 5 cut(s) 24, 2334, 2342, 2478, 3090
StyI CCWWGG 2 cut(s) 1701, 2596
TaaI ACNGT 6 cut(s) 1030, 1682, 1963, 2411, 2861, 3243
TaiI ACGT 4 cut(s) 186, 355, 2422, 3119
TatI WGTACW 4 cut(s) 522, 1573, 1796, 2170
TauI GCSGC 3 cut(s) 502, 2663, 2990
TfiI GAWTC 7 cut(s) 167, 464, 617, 821, 1145, 1675, 2711
Tru1I TTAA 7 cut(s) 728, 933, 2214, 2289, 2625, 2631, 3269
Tru9I TTAA 7 cut(s) 728, 933, 2214, 2289, 2625, 2631, 3269
TscAI CASTG 4 cut(s) 1506, 1687, 2329, 2962
TseFI GTSAC 2 cut(s) 131, 3100
Tsp45I GTSAC 2 cut(s) 131, 3100
TspGWI ACGGA 3 cut(s) 685, 1280, 1749
TspRI CASTG 4 cut(s) 1506, 1687, 2329, 2962
Vha464I CTTAAG 1 cut(s) 2213
VpaK11BI GGWCC 3 cut(s) 1165, 1600, 3194
VspI ATTAAT 1 cut(s) 933
XapI RAATTY 4 cut(s) 1298, 2157, 2371, 2627
XmiI GTMKAC 1 cut(s) 1731
XmnI GAANNNNTTC 1 cut(s) 2733
XspI CTAG 5 cut(s) 24, 2334, 2342, 2478, 3090
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.