Cytochrome p450
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 3 cut(s) | 429, 1026, 1317 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 182, 1069 |
| AccI | GTMKAC | 2 cut(s) | 864, 918 |
| AciI | CCGC | 8 cut(s) | 112, 217, 309, 352, 494, 543, 551, 1489 |
| AclWI | GGATC | 3 cut(s) | 813, 1089, 1265 |
| AcoI | YGGCCR | 1 cut(s) | 1011 |
| AcuI | CTGAAG | 3 cut(s) | 42, 969, 1132 |
| AcyI | GRCGYC | 1 cut(s) | 430 |
| AfaI | GTAC | 5 cut(s) | 245, 377, 386, 608, 837 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 99, 135, 182, 1069 |
| AflIII | ACRYGT | 2 cut(s) | 378, 921 |
| AgsI | TTSAA | 4 cut(s) | 458, 694, 1094, 1384 |
| AjiI | CACGTC | 1 cut(s) | 868 |
| AjnI | CCWGG | 2 cut(s) | 157, 1008 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 659, 691 |
| AluBI | AGCT | 9 cut(s) | 445, 589, 689, 1060, 1114, 1146, 1288, 1358, 1393 |
| AluI | AGCT | 9 cut(s) | 445, 589, 689, 1060, 1114, 1146, 1288, 1358, 1393 |
| Alw21I | GWGCWC | 3 cut(s) | 1062, 1377, 1395 |
| Alw26I | GTCTC | 3 cut(s) | 108, 298, 1052 |
| Alw44I | GTGCAC | 1 cut(s) | 1373 |
| AlwI | GGATC | 3 cut(s) | 813, 1089, 1265 |
| AoxI | GGCC | 4 cut(s) | 160, 396, 402, 1011 |
| ApaLI | GTGCAC | 1 cut(s) | 1373 |
| ApeKI | GCWGC | 3 cut(s) | 219, 871, 1408 |
| ArsI | GACNNNNNNTTYG | 4 cut(s) | 326, 358, 770, 802 |
| AseI | ATTAAT | 1 cut(s) | 881 |
| AspA2I | CCTAGG | 1 cut(s) | 268 |
| AspLEI | GCGC | 3 cut(s) | 267, 432, 753 |
| AspS9I | GGNCC | 7 cut(s) | 161, 248, 419, 778, 1005, 1166, 1257 |
| AsuC2I | CCSGG | 1 cut(s) | 1031 |
| AsuHPI | GGTGA | 4 cut(s) | 20, 566, 596, 713 |
| AsuII | TTCGAA | 3 cut(s) | 439, 789, 927 |
| AsuNHI | GCTAGC | 1 cut(s) | 347 |
| AvaII | GGWCC | 6 cut(s) | 248, 419, 778, 1005, 1166, 1257 |
| AvrII | CCTAGG | 1 cut(s) | 268 |
| BaeGI | GKGCMC | 2 cut(s) | 1322, 1377 |
| BanI | GGYRCC | 3 cut(s) | 429, 1026, 1317 |
| BanII | GRGCYC | 2 cut(s) | 1062, 1395 |
| BbsI | GAAGAC | 2 cut(s) | 76, 1234 |
| Bbv12I | GWGCWC | 3 cut(s) | 1062, 1377, 1395 |
| BbvI | GCAGC | 3 cut(s) | 231, 858, 1395 |
| BccI | CCATC | 4 cut(s) | 77, 607, 1072, 1205 |
| BceAI | ACGGC | 1 cut(s) | 1008 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 429, 463, 1299, 1333 |
| BciT130I | CCWGG | 2 cut(s) | 159, 1010 |
| BcnI | CCSGG | 1 cut(s) | 1031 |
| BcoDI | GTCTC | 3 cut(s) | 108, 298, 1052 |
| BfaI | CTAG | 6 cut(s) | 200, 233, 269, 348, 822, 1355 |
| BfoI | RGCGCY | 1 cut(s) | 433 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 548 |
| BglII | AGATCT | 1 cut(s) | 565 |
| BisI | GCNGC | 6 cut(s) | 220, 309, 352, 543, 872, 1409 |
| BlnI | CCTAGG | 1 cut(s) | 268 |
| BlsI | GCNGC | 6 cut(s) | 221, 310, 353, 544, 873, 1410 |
| Bme1390I | CCNGG | 3 cut(s) | 159, 1010, 1031 |
| Bme18I | GGWCC | 6 cut(s) | 248, 419, 778, 1005, 1166, 1257 |
| BmgBI | CACGTC | 1 cut(s) | 868 |
| BmgT120I | GGNCC | 7 cut(s) | 161, 248, 419, 778, 1005, 1166, 1257 |
| BmiI | GGNNCC | 6 cut(s) | 250, 431, 973, 1007, 1028, 1319 |
| BmrFI | CCNGG | 3 cut(s) | 159, 1010, 1031 |
| BmrI | ACTGGG | 1 cut(s) | 1396 |
| BmsI | GCATC | 1 cut(s) | 123 |
| BmtI | GCTAGC | 1 cut(s) | 351 |
| BmuI | ACTGGG | 1 cut(s) | 1396 |
| BpiI | GAAGAC | 2 cut(s) | 76, 1234 |
| BpmI | CTGGAG | 1 cut(s) | 209 |
| Bpu14I | TTCGAA | 3 cut(s) | 439, 789, 927 |
| BpuEI | CTTGAG | 4 cut(s) | 118, 635, 1042, 1144 |
| BpuMI | CCSGG | 1 cut(s) | 1031 |
| BsaAI | YACGTR | 1 cut(s) | 606 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1215 |
| BsaHI | GRCGYC | 1 cut(s) | 430 |
| BsaI | GGTCTC | 1 cut(s) | 108 |
| BsaJI | CCNNGG | 5 cut(s) | 268, 399, 990, 1008, 1191 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 500, 530, 659, 689 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 99, 135, 182, 1069 |
| Bse1I | ACTGG | 2 cut(s) | 1391, 1405 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1215 |
| BseBI | CCWGG | 2 cut(s) | 159, 1010 |
| BseDI | CCNNGG | 5 cut(s) | 268, 399, 990, 1008, 1191 |
| BseGI | GGATG | 5 cut(s) | 69, 114, 296, 618, 1083 |
| BseJI | GATNNNNATC | 1 cut(s) | 1215 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 99, 135, 182, 1069 |
| BseMII | CTCAG | 2 cut(s) | 303, 488 |
| BseNI | ACTGG | 2 cut(s) | 1391, 1405 |
| BseRI | GAGGAG | 3 cut(s) | 121, 521, 680 |
| BseSI | GKGCMC | 2 cut(s) | 1322, 1377 |
| BseXI | GCAGC | 3 cut(s) | 231, 858, 1395 |
| BsgI | GTGCAG | 2 cut(s) | 433, 545 |
| BshFI | GGCC | 4 cut(s) | 162, 398, 404, 1013 |
| BshNI | GGYRCC | 3 cut(s) | 429, 1026, 1317 |
| BsiHKAI | GWGCWC | 3 cut(s) | 1062, 1377, 1395 |
| BsiSI | CCGG | 1 cut(s) | 1031 |
| BsiWI | CGTACG | 1 cut(s) | 384 |
| BslFI | GGGAC | 1 cut(s) | 514 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 99, 135, 182, 1069 |
| BsmAI | GTCTC | 3 cut(s) | 108, 298, 1052 |
| BsmBI | CGTCTC | 1 cut(s) | 1052 |
| BsmFI | GGGAC | 1 cut(s) | 514 |
| BsnI | GGCC | 4 cut(s) | 162, 398, 404, 1013 |
| Bso31I | GGTCTC | 1 cut(s) | 108 |
| Bsp119I | TTCGAA | 3 cut(s) | 439, 789, 927 |
| Bsp1286I | GDGCHC | 4 cut(s) | 1062, 1322, 1377, 1395 |
| Bsp143I | GATC | 6 cut(s) | 23, 565, 646, 805, 1081, 1270 |
| Bsp19I | CCATGG | 2 cut(s) | 399, 1191 |
| BspACI | CCGC | 8 cut(s) | 112, 217, 309, 352, 494, 543, 551, 1489 |
| BspANI | GGCC | 4 cut(s) | 162, 398, 404, 1013 |
| BspCNI | CTCAG | 2 cut(s) | 304, 489 |
| BspHI | TCATGA | 1 cut(s) | 808 |
| BspLI | GGNNCC | 6 cut(s) | 250, 431, 973, 1007, 1028, 1319 |
| BspOI | GCTAGC | 1 cut(s) | 351 |
| BspPI | GGATC | 3 cut(s) | 813, 1089, 1265 |
| BspT104I | TTCGAA | 3 cut(s) | 439, 789, 927 |
| BspT107I | GGYRCC | 3 cut(s) | 429, 1026, 1317 |
| BspTNI | GGTCTC | 1 cut(s) | 108 |
| BsrI | ACTGG | 2 cut(s) | 1391, 1405 |
| BssECI | CCNNGG | 5 cut(s) | 268, 399, 990, 1008, 1191 |
| BssMI | GATC | 6 cut(s) | 23, 565, 646, 805, 1081, 1270 |
| BssNAI | GTATAC | 1 cut(s) | 919 |
| BssNI | GRCGYC | 1 cut(s) | 430 |
| BssT1I | CCWWGG | 3 cut(s) | 268, 399, 1191 |
| Bst1107I | GTATAC | 1 cut(s) | 919 |
| Bst2UI | CCWGG | 2 cut(s) | 159, 1010 |
| Bst4CI | ACNGT | 2 cut(s) | 775, 1348 |
| Bst6I | CTCTTC | 3 cut(s) | 111, 752, 1306 |
| BstACI | GRCGYC | 1 cut(s) | 430 |
| BstBAI | YACGTR | 1 cut(s) | 606 |
| BstBI | TTCGAA | 3 cut(s) | 439, 789, 927 |
| BstC8I | GCNNGC | 4 cut(s) | 349, 452, 1406, 1473 |
| BstDEI | CTNAG | 3 cut(s) | 312, 497, 569 |
| BstDSI | CCRYGG | 3 cut(s) | 399, 990, 1191 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 133 |
| BstF5I | GGATG | 5 cut(s) | 69, 114, 296, 618, 1083 |
| BstH2I | RGCGCY | 1 cut(s) | 433 |
| BstHHI | GCGC | 3 cut(s) | 267, 432, 753 |
| BstKTI | GATC | 6 cut(s) | 26, 568, 649, 808, 1084, 1273 |
| BstMAI | GTCTC | 3 cut(s) | 108, 298, 1052 |
| BstMBI | GATC | 6 cut(s) | 23, 565, 646, 805, 1081, 1270 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 273, 451, 548, 1477 |
| BstNI | CCWGG | 2 cut(s) | 159, 1010 |
| BstNSI | RCATGY | 3 cut(s) | 382, 925, 1408 |
| BstSCI | CCNGG | 3 cut(s) | 157, 1008, 1029 |
| BstSLI | GKGCMC | 2 cut(s) | 1322, 1377 |
| BstV1I | GCAGC | 3 cut(s) | 231, 858, 1395 |
| BstV2I | GAAGAC | 2 cut(s) | 76, 1234 |
| BstX2I | RGATCY | 2 cut(s) | 565, 1270 |
| BstYI | RGATCY | 2 cut(s) | 565, 1270 |
| BstZ17I | GTATAC | 1 cut(s) | 919 |
| BsuRI | GGCC | 4 cut(s) | 162, 398, 404, 1013 |
| BtgI | CCRYGG | 3 cut(s) | 399, 990, 1191 |
| BtrI | CACGTC | 1 cut(s) | 868 |
| BtsCI | GGATG | 5 cut(s) | 69, 114, 296, 618, 1083 |
| BtsI | GCAGTG | 1 cut(s) | 1375 |
| BtsIMutI | CAGTG | 1 cut(s) | 1375 |
| Cac8I | GCNNGC | 4 cut(s) | 349, 452, 1406, 1473 |
| CciI | TCATGA | 1 cut(s) | 808 |
| CfoI | GCGC | 3 cut(s) | 267, 432, 753 |
| Cfr13I | GGNCC | 7 cut(s) | 161, 248, 419, 778, 1005, 1166, 1257 |
| CseI | GACGC | 1 cut(s) | 1492 |
| Csp6I | GTAC | 5 cut(s) | 244, 376, 385, 607, 836 |
| CviQI | GTAC | 5 cut(s) | 244, 376, 385, 607, 836 |
| DdeI | CTNAG | 3 cut(s) | 312, 497, 569 |
| DinI | GGCGCC | 1 cut(s) | 431 |
| DpnI | GATC | 6 cut(s) | 25, 567, 648, 807, 1083, 1272 |
| DpnII | GATC | 6 cut(s) | 23, 565, 646, 805, 1081, 1270 |
| EaeI | YGGCCR | 1 cut(s) | 1011 |
| Eam1104I | CTCTTC | 3 cut(s) | 111, 752, 1306 |
| EarI | CTCTTC | 3 cut(s) | 111, 752, 1306 |
| Ecl136II | GAGCTC | 2 cut(s) | 1060, 1393 |
| Eco130I | CCWWGG | 3 cut(s) | 268, 399, 1191 |
| Eco24I | GRGCYC | 2 cut(s) | 1062, 1395 |
| Eco31I | GGTCTC | 1 cut(s) | 108 |
| Eco32I | GATATC | 1 cut(s) | 1217 |
| Eco47I | GGWCC | 6 cut(s) | 248, 419, 778, 1005, 1166, 1257 |
| Eco53kI | GAGCTC | 2 cut(s) | 1060, 1393 |
| Eco57I | CTGAAG | 3 cut(s) | 42, 969, 1132 |
| EcoICRI | GAGCTC | 2 cut(s) | 1060, 1393 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 133 |
| EcoRII | CCWGG | 2 cut(s) | 157, 1008 |
| EcoRV | GATATC | 1 cut(s) | 1217 |
| EcoT14I | CCWWGG | 3 cut(s) | 268, 399, 1191 |
| EcoT38I | GRGCYC | 2 cut(s) | 1062, 1395 |
| EgeI | GGCGCC | 1 cut(s) | 431 |
| EheI | GGCGCC | 1 cut(s) | 431 |
| ErhI | CCWWGG | 3 cut(s) | 268, 399, 1191 |
| Esp3I | CGTCTC | 1 cut(s) | 1052 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 1130, 1162, 1427, 1459 |
| FaqI | GGGAC | 1 cut(s) | 514 |
| FauI | CCCGC | 1 cut(s) | 544 |
| FauNDI | CATATG | 1 cut(s) | 195 |
| FblI | GTMKAC | 2 cut(s) | 864, 918 |
| Fnu4HI | GCNGC | 6 cut(s) | 220, 309, 352, 543, 872, 1409 |
| FokI | GGATG | 5 cut(s) | 56, 101, 283, 625, 1090 |
| FriOI | GRGCYC | 2 cut(s) | 1062, 1395 |
| Fsp4HI | GCNGC | 6 cut(s) | 220, 309, 352, 543, 872, 1409 |
| FspBI | CTAG | 6 cut(s) | 200, 233, 269, 348, 822, 1355 |
| GlaI | GCGC | 3 cut(s) | 266, 431, 752 |
| GluI | GCNGC | 6 cut(s) | 220, 309, 352, 543, 872, 1409 |
| GsuI | CTGGAG | 1 cut(s) | 209 |
| HaeII | RGCGCY | 1 cut(s) | 433 |
| HaeIII | GGCC | 4 cut(s) | 162, 398, 404, 1013 |
| HapII | CCGG | 1 cut(s) | 1031 |
| HgaI | GACGC | 1 cut(s) | 1492 |
| HhaI | GCGC | 3 cut(s) | 267, 432, 753 |
| Hin1I | GRCGYC | 1 cut(s) | 430 |
| Hin6I | GCGC | 3 cut(s) | 265, 430, 751 |
| HinP1I | GCGC | 3 cut(s) | 265, 430, 751 |
| HincII | GTYRAC | 1 cut(s) | 1341 |
| HindII | GTYRAC | 1 cut(s) | 1341 |
| HinfI | GANTC | 4 cut(s) | 52, 477, 600, 1239 |
| HpaII | CCGG | 1 cut(s) | 1031 |
| HphI | GGTGA | 4 cut(s) | 20, 566, 596, 713 |
| Hpy166II | GTNNAC | 5 cut(s) | 778, 865, 919, 1341, 1375 |
| Hpy188I | TCNGA | 2 cut(s) | 61, 412 |
| Hpy8I | GTNNAC | 5 cut(s) | 778, 865, 919, 1341, 1375 |
| Hpy99I | CGWCG | 1 cut(s) | 1486 |
| HpyAV | CCTTC | 4 cut(s) | 66, 103, 388, 495 |
| HpyCH4III | ACNGT | 2 cut(s) | 775, 1348 |
| HpyCH4IV | ACGT | 5 cut(s) | 10, 605, 867, 1045, 1200 |
| HpyCH4V | TGCA | 4 cut(s) | 450, 526, 1330, 1375 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 273, 451, 548, 1477 |
| HpyF3I | CTNAG | 3 cut(s) | 312, 497, 569 |
| HpySE526I | ACGT | 5 cut(s) | 10, 605, 867, 1045, 1200 |
| Hsp92I | GRCGYC | 1 cut(s) | 430 |
| HspAI | GCGC | 3 cut(s) | 265, 430, 751 |
| KasI | GGCGCC | 1 cut(s) | 429 |
| Kzo9I | GATC | 6 cut(s) | 23, 565, 646, 805, 1081, 1270 |
| LmnI | GCTCC | 5 cut(s) | 496, 686, 1065, 1390, 1398 |
| Lsp1109I | GCAGC | 3 cut(s) | 231, 858, 1395 |
| LweI | GCATC | 1 cut(s) | 123 |
| MaeI | CTAG | 6 cut(s) | 200, 233, 269, 348, 822, 1355 |
| MaeII | ACGT | 5 cut(s) | 10, 605, 867, 1045, 1200 |
| MalI | GATC | 6 cut(s) | 25, 567, 648, 807, 1083, 1272 |
| MboI | GATC | 6 cut(s) | 23, 565, 646, 805, 1081, 1270 |
| MboII | GAAGA | 7 cut(s) | 76, 98, 182, 769, 1106, 1234, 1323 |
| MflI | RGATCY | 2 cut(s) | 565, 1270 |
| MhlI | GDGCHC | 4 cut(s) | 1062, 1322, 1377, 1395 |
| MluCI | AATT | 2 cut(s) | 762, 798 |
| Mly113I | GGCGCC | 1 cut(s) | 430 |
| MlyI | GAGTC | 2 cut(s) | 61, 1248 |
| MmeI | TCCRAC | 1 cut(s) | 1144 |
| MseI | TTAA | 3 cut(s) | 324, 881, 896 |
| MspA1I | CMGCKG | 2 cut(s) | 311, 496 |
| MspI | CCGG | 1 cut(s) | 1031 |
| MspR9I | CCNGG | 3 cut(s) | 159, 1010, 1031 |
| MvaI | CCWGG | 2 cut(s) | 159, 1010 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 273, 451, 548, 1477 |
| NarI | GGCGCC | 1 cut(s) | 430 |
| NciI | CCSGG | 1 cut(s) | 1031 |
| NcoI | CCATGG | 2 cut(s) | 399, 1191 |
| NdeI | CATATG | 1 cut(s) | 195 |
| NdeII | GATC | 6 cut(s) | 23, 565, 646, 805, 1081, 1270 |
| NheI | GCTAGC | 1 cut(s) | 347 |
| NlaIV | GGNNCC | 6 cut(s) | 250, 431, 973, 1007, 1028, 1319 |
| NspI | RCATGY | 3 cut(s) | 382, 925, 1408 |
| NspV | TTCGAA | 3 cut(s) | 439, 789, 927 |
| PaeI | GCATGC | 1 cut(s) | 1408 |
| PagI | TCATGA | 1 cut(s) | 808 |
| PciI | ACATGT | 2 cut(s) | 378, 921 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 389 |
| PfeI | GAWTC | 2 cut(s) | 477, 600 |
| Pfl23II | CGTACG | 1 cut(s) | 384 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 182, 1069 |
| PkrI | GCNGC | 6 cut(s) | 221, 310, 353, 544, 873, 1410 |
| PleI | GAGTC | 2 cut(s) | 60, 1247 |
| PluTI | GGCGCC | 1 cut(s) | 433 |
| PpsI | GAGTC | 2 cut(s) | 60, 1247 |
| Ppu21I | YACGTR | 1 cut(s) | 606 |
| PscI | ACATGT | 2 cut(s) | 378, 921 |
| PshBI | ATTAAT | 1 cut(s) | 881 |
| Psp124BI | GAGCTC | 2 cut(s) | 1062, 1395 |
| Psp6I | CCWGG | 2 cut(s) | 157, 1008 |
| PspGI | CCWGG | 2 cut(s) | 157, 1008 |
| PspLI | CGTACG | 1 cut(s) | 384 |
| PspN4I | GGNNCC | 6 cut(s) | 250, 431, 973, 1007, 1028, 1319 |
| PspPI | GGNCC | 7 cut(s) | 161, 248, 419, 778, 1005, 1166, 1257 |
| PsuI | RGATCY | 2 cut(s) | 565, 1270 |
| RsaI | GTAC | 5 cut(s) | 245, 377, 386, 608, 837 |
| RsaNI | GTAC | 5 cut(s) | 244, 376, 385, 607, 836 |
| SacI | GAGCTC | 2 cut(s) | 1062, 1395 |
| SaqAI | TTAA | 3 cut(s) | 324, 881, 896 |
| SatI | GCNGC | 6 cut(s) | 220, 309, 352, 543, 872, 1409 |
| Sau3AI | GATC | 6 cut(s) | 23, 565, 646, 805, 1081, 1270 |
| Sau96I | GGNCC | 7 cut(s) | 161, 248, 419, 778, 1005, 1166, 1257 |
| SchI | GAGTC | 2 cut(s) | 61, 1248 |
| ScrFI | CCNGG | 3 cut(s) | 159, 1010, 1031 |
| SduI | GDGCHC | 4 cut(s) | 1062, 1322, 1377, 1395 |
| SfaNI | GCATC | 1 cut(s) | 123 |
| SfoI | GGCGCC | 1 cut(s) | 431 |
| SfuI | TTCGAA | 3 cut(s) | 439, 789, 927 |
| SinI | GGWCC | 6 cut(s) | 248, 419, 778, 1005, 1166, 1257 |
| SmlI | CTYRAG | 4 cut(s) | 133, 650, 1021, 1123 |
| SmoI | CTYRAG | 4 cut(s) | 133, 650, 1021, 1123 |
| SphI | GCATGC | 1 cut(s) | 1408 |
| Sse9I | AATT | 2 cut(s) | 762, 798 |
| SsiI | CCGC | 8 cut(s) | 112, 217, 309, 352, 494, 543, 551, 1489 |
| SspDI | GGCGCC | 1 cut(s) | 429 |
| SspI | AATATT | 1 cut(s) | 1252 |
| SspMI | CTAG | 6 cut(s) | 200, 233, 269, 348, 822, 1355 |
| SstI | GAGCTC | 2 cut(s) | 1062, 1395 |
| StyD4I | CCNGG | 3 cut(s) | 157, 1008, 1029 |
| StyI | CCWWGG | 3 cut(s) | 268, 399, 1191 |
| TaaI | ACNGT | 2 cut(s) | 775, 1348 |
| TaiI | ACGT | 5 cut(s) | 13, 608, 870, 1048, 1203 |
| TaqI | TCGA | 9 cut(s) | 22, 439, 480, 789, 927, 957, 1084, 1309, 1454 |
| TasI | AATT | 2 cut(s) | 762, 798 |
| TatI | WGTACW | 2 cut(s) | 375, 835 |
| TauI | GCSGC | 3 cut(s) | 311, 354, 545 |
| TfiI | GAWTC | 2 cut(s) | 477, 600 |
| Tru1I | TTAA | 3 cut(s) | 324, 881, 896 |
| Tru9I | TTAA | 3 cut(s) | 324, 881, 896 |
| TscAI | CASTG | 1 cut(s) | 1382 |
| TseI | GCWGC | 3 cut(s) | 219, 871, 1408 |
| TspDTI | ATGAA | 6 cut(s) | 182, 770, 825, 867, 1055, 1234 |
| TspGWI | ACGGA | 1 cut(s) | 261 |
| TspRI | CASTG | 1 cut(s) | 1382 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 182, 1069 |
| VneI | GTGCAC | 1 cut(s) | 1373 |
| VpaK11BI | GGWCC | 6 cut(s) | 248, 419, 778, 1005, 1166, 1257 |
| VspI | ATTAAT | 1 cut(s) | 881 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 133 |
| XbaI | TCTAGA | 1 cut(s) | 821 |
| XceI | RCATGY | 3 cut(s) | 382, 925, 1408 |
| XmaJI | CCTAGG | 1 cut(s) | 268 |
| XmiI | GTMKAC | 2 cut(s) | 864, 918 |
| XspI | CTAG | 6 cut(s) | 200, 233, 269, 348, 822, 1355 |