pycom14g18600

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0000282
Physical Location & Seq
Forward (+)
20190403 .. 20193472
3070 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g18600.1

Sequence Viewer

Length: 1560 bp
ATGTCACATTTCTCGATCTCACCTAGAAATCAAGAAAAAATGAGTCCTTCAGAAATAGCCATCCTAATCTTTGTCTTCCTTGCCTTCTTATGGTCCCTCCTCCACATCCTAATCAACGCCTCCTCAAGGCAAAGCCAAAAACTGCCTCCAGGCCCTAGGGCTCTACCAATCATTGGCAATCTTCATATGCTAGGCAACCTCCCACACCGCAGCCTCCAGAACCTAGCCAAAAAGTACGGACCCATAATGTCCATGCGCCTAGGCAGCAAAACCACCATAGTGGTCTCATCCCCCAAAGCCGCTGAACTATTCCTTAAAACCCACGACACCATTTTCGCTAGCCGCCCCCAACTCCAATCCTCCAAGCACATGTCCTACGGCACGAAGGCCATGGCCTTTTCTGAATATGGTCCATATTGGCGCCATGTTCGAAAGCTCTGCACACTTCAACTTCTTTCTCCTTCGAGAATCGAGGCTTTTGCTCCGTTGAGAAGGGAGGAGGTAGGGTTGTTGGTGCAGTCCCTGAAGGTAGCTGCGGAGGCGGGTGAGGTGGTTGATCTTAGTGAGAAGGTCAGTGATCTTGTTGTGGGAATCACGTACAGGATGGTGTTGGGGAGAAAAAATAATGATATGTTTGATCTCAAAGGGATTATAGAGGAGGTCATGTTCTTGTCAGGAGCTTTCAACATTGGTGATTATGTGCCTTTCCTTATTCCACTTGATCTTCAGGGATTGACCAAGCGCATGAAGAGAATTAGCAAGACTGTCGACCAACTATTCGAAAAGATAATTCAGGATCATGAACAAGTTTCTAGAAGTGAGCAAGTACAAGGCAATCATCATAAGGACTTTGTAGACGTGCTGCTTTCATTAATCCACCAACCATTAAACCCCAACGATGAGGAAGTATACATGTTCGAACGAACAAATGCCAAAGCCATTTTACTCGATATGATTTCTGGTTCCTTTGACACTTCCGCCACGGCAATCGTCTGGACCCTGGCCGAACTCTTGAGGCACCCGGAAGTTATGAAACGTCTCCAAAAAGAGCTCCAGAGTGTGGTCGGGATGGATCGAATGGTTGAAGAAAGTGATTTGCCAAAGCTGGATTACTTGAGCATGGTGGTCAAGGAGAGCTTAAGGCTACACCCAGTTGGACCATTACTTATCCCTCATCAATCCATGGAGGACGTTATAGTTGATGGATATCACATACCCAAGAAATCAAGAGTCTTCATCAATATTTGGACCATTGGGAGAGATCCAAATGTATGGTCAGATACTGTGGAAGAATTCTACCCTGAAAGGTTTATGAATAGTAACGTTGACCTGCAGGGACATGACTTCCAGCTCATACCATTTGGCTCTGGTCGAAGAGGGTGCCCTGCTATGCAGTTAGGGTTAACCACAGTTCGTCTAGCTCTGGGAAACTTGGTGCACTGCTTCAACTGGGAGCTCCCAACTGGCATGCTACCTACAGACTTGGACATGACTGAAAAGTTCGGCTTATCGTTGTCGAAAGCCAAACACTTGCTTGCCATGCCAACGCGCCGCCTATAA

Protein Analysis

520

Amino Acids

58.77

Weight (kDa)

8.54

Isoelectric Point (pI)

54.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 49 - 508 2.8e-101 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000282)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g12350 FvH4_5g12380 FvH4_5g12392 FvH4_5g12400 FvH4_5g12430
malus_domestica MD06G1213300.v1.1 MD06G1213500.v1.1 MD06G1213700.v1.1 MD06G1213800.v1.1 MD06G1213900.v1.1 MD06G1214000.v1.1 MD06G1214400.v1.1 MD14G1224300.v1.1 MD14G1224400.v1.1 MD14G1224600.v1.1 MD14G1224700.v1.1 MD14G1224800.v1.1 MD14G1224900.v1.1 MD14G1225100.v1.1 MD14G1225400.v1.1
prunus_persica Prupe.5G219700_v2.0.a1 Prupe.5G219800_v2.0.a1 Prupe.5G219900_v2.0.a1 Prupe.8G078500_v2.0.a1
pyrus_communis pycom06g19100 pycom14g18590 pycom14g18600 pycom14g18610 pycom14g18660
rosa_chinensis RchiOBHm_Chr0c12g0499591 RchiOBHm_Chr2g0140591 RchiOBHm_Chr2g0140631 RchiOBHm_Chr2g0140641 RchiOBHm_Chr2g0140651 RchiOBHm_Chr4g0417991 RchiOBHm_Chr4g0418001 RchiOBHm_Chr4g0418101 RchiOBHm_Chr4g0418111 RchiOBHm_Chr4g0418121 RchiOBHm_Chr6g0280191 RchiOBHm_Chr6g0280241 RchiOBHm_Chr6g0280251 RchiOBHm_Chr7g0185031 RchiOBHm_Chr7g0185051 RchiOBHm_Chr7g0185081 RchiOBHm_Chr7g0185091 RchiOBHm_Chr7g0185111 RchiOBHm_Chr7g0185131 RchiOBHm_Chr7g0185151 RchiOBHm_Chr7g0185161 RchiOBHm_Chr7g0185191 RchiOBHm_Chr7g0222431
rosa_laevigata RLG00000002061 RLG00000004934 RLG00000004935 RLG00000004937 RLG00000013071 RLG00000013077 RLG00000019621 RLG00000019881 RLG00000019884 RLG00000019887 RLG00000019888 RLG00000028839
rosa_multiflora Rmu_co8014370.1_g000001 Rmu_co8334683.1_g000001 Rmu_co8440383.1_g000001 Rmu_sc0002826.1_g000008 Rmu_sc0002826.1_g000009 Rmu_sc0002826.1_g000030 Rmu_sc0004483.1_g000005 Rmu_sc0004638.1_g000003 Rmu_sc0004638.1_g000004 Rmu_sc0005756.1_g000003 Rmu_sc0006493.1_g000023 Rmu_sc0006493.1_g000024 Rmu_sc0007137.1_g000017 Rmu_sc0008496.1_g000022 Rmu_sc0008769.1_g000003 Rmu_sc0013612.1_g000026 Rmu_sc0013864.1_g000001 Rmu_sc0013864.1_g000007 Rmu_sc0013864.1_g000008 Rmu_sc0030128.1_g000001 Rmu_sc0041660.1_g000001 Rmu_ssc0000289.1_g000001
rosa_roxburghii Rroxscaffold_2G00104640 Rroxscaffold_3G00253970 Rroxscaffold_3G00269170 Rroxscaffold_3G00269200 Rroxscaffold_3G00269210 Rroxscaffold_3G00269220 Rroxscaffold_3G00269230 Rroxscaffold_7G00188320
rosa_rugosa Rorug01G0052300 Rorug05G0212000 Rorug06G0130700 Rorug06G0131100 Rorug06G0466200 Rorug06G0466300 Rorug06G0466400 Rorug06G0466500 Rorug06G0466700 Rorug07G0208500 Rorug07G0294600
rosa_samantha Rh4AG137600 Rh6DG237300 Rh6DG237800 Rh7AG076200 Rh7AG076300 Rh7AG076500 Rh7AG076600 Rh7AG350700 Rh7BG070800 Rh7BG070900 Rh7BG071000 Rh7BG071100
rosa_wichuraiana Rw6G020990 Rw6G021010 Rw7G005900 Rw7G005910 Rw7G005920 Rw7G005940 Rw7G005950 Rw7G005970 Rw7G029600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1338
AccB1I GGYRCC 3 cut(s) 420, 1017, 1380
AccB7I CCANNNNNTGG 2 cut(s) 173, 1060
AccI GTMKAC 3 cut(s) 768, 855, 909
AccII CGCG 1 cut(s) 1549
AciI CCGC 7 cut(s) 208, 300, 343, 536, 542, 978, 1552
AclI AACGTT 1 cut(s) 1323
AclWI GGATC 3 cut(s) 804, 1080, 1256
AcoI YGGCCR 1 cut(s) 1002
AcsI RAATTY 1 cut(s) 1292
AcuI CTGAAG 3 cut(s) 33, 545, 710
AcyI GRCGYC 1 cut(s) 421
AfaI GTAC 3 cut(s) 236, 599, 828
AfiI CCNNNNNNNGG 4 cut(s) 90, 126, 173, 1060
AflII CTTAAG 1 cut(s) 1138
AflIII ACRYGT 2 cut(s) 369, 912
AgsI TTSAA 4 cut(s) 449, 685, 1085, 1447
AjiI CACGTC 1 cut(s) 859
AjnI CCWGG 2 cut(s) 148, 999
AleI CACNNNNGTG 1 cut(s) 278
AloI GAACNNNNNNTCC 2 cut(s) 650, 682
AluBI AGCT 9 cut(s) 436, 533, 680, 1051, 1105, 1137, 1351, 1421, 1456
AluI AGCT 9 cut(s) 436, 533, 680, 1051, 1105, 1137, 1351, 1421, 1456
Alw21I GWGCWC 3 cut(s) 1053, 1440, 1458
Alw26I GTCTC 2 cut(s) 289, 1043
Alw44I GTGCAC 1 cut(s) 1436
AlwI GGATC 3 cut(s) 804, 1080, 1256
AlwNI CAGNNNCTG 2 cut(s) 523, 1283
AoxI GGCC 4 cut(s) 151, 387, 393, 1002
ApaLI GTGCAC 1 cut(s) 1436
ApeKI GCWGC 4 cut(s) 210, 264, 533, 862
ApoI RAATTY 1 cut(s) 1292
ArsI GACNNNNNNTTYG 6 cut(s) 317, 349, 761, 793, 1259, 1291
AseI ATTAAT 1 cut(s) 872
AspA2I CCTAGG 2 cut(s) 155, 259
AspLEI GCGC 4 cut(s) 258, 423, 744, 1551
AspS9I GGNCC 7 cut(s) 93, 152, 239, 410, 996, 1157, 1248
AsuC2I CCSGG 1 cut(s) 1022
AsuHPI GGTGA 3 cut(s) 12, 557, 704
AsuII TTCGAA 3 cut(s) 430, 780, 918
AsuNHI GCTAGC 1 cut(s) 338
AvaII GGWCC 6 cut(s) 93, 239, 410, 996, 1157, 1248
AvrII CCTAGG 2 cut(s) 155, 259
BaeGI GKGCMC 2 cut(s) 1385, 1440
BanI GGYRCC 3 cut(s) 420, 1017, 1380
BanII GRGCYC 3 cut(s) 163, 1053, 1458
BbsI GAAGAC 2 cut(s) 67, 1225
Bbv12I GWGCWC 3 cut(s) 1053, 1440, 1458
BbvI GCAGC 4 cut(s) 222, 276, 520, 849
BccI CCATC 4 cut(s) 68, 598, 1063, 1196
BceAI ACGGC 2 cut(s) 394, 999
BcgI CGANNNNNNTGC 8 cut(s) 420, 454, 461, 495, 748, 782, 1362, 1396
BciT130I CCWGG 2 cut(s) 150, 1001
BcnI CCSGG 1 cut(s) 1022
BcoDI GTCTC 2 cut(s) 289, 1043
BfaI CTAG 8 cut(s) 24, 156, 191, 224, 260, 339, 813, 1418
BfmI CTRYAG 2 cut(s) 1331, 1476
BfoI RGCGCY 1 cut(s) 424
BfrI CTTAAG 1 cut(s) 1138
BfuAI ACCTGC 1 cut(s) 1338
BisI GCNGC 7 cut(s) 211, 265, 300, 343, 534, 863, 1552
BlnI CCTAGG 2 cut(s) 155, 259
BlsI GCNGC 7 cut(s) 212, 266, 301, 344, 535, 864, 1553
Bme1390I CCNGG 3 cut(s) 150, 1001, 1022
Bme18I GGWCC 6 cut(s) 93, 239, 410, 996, 1157, 1248
BmgBI CACGTC 1 cut(s) 859
BmgT120I GGNCC 7 cut(s) 93, 152, 239, 410, 996, 1157, 1248
BmiI GGNNCC 7 cut(s) 95, 241, 422, 964, 998, 1019, 1382
BmrFI CCNGG 3 cut(s) 150, 1001, 1022
BmrI ACTGGG 2 cut(s) 1145, 1459
BmtI GCTAGC 1 cut(s) 342
BmuI ACTGGG 2 cut(s) 1145, 1459
BpiI GAAGAC 2 cut(s) 67, 1225
BpmI CTGGAG 3 cut(s) 132, 200, 1037
Bpu14I TTCGAA 3 cut(s) 430, 780, 918
BpuEI CTTGAG 3 cut(s) 109, 1033, 1135
BpuMI CCSGG 1 cut(s) 1022
BsaAI YACGTR 1 cut(s) 597
BsaBI GATNNNNATC 1 cut(s) 1206
BsaHI GRCGYC 1 cut(s) 421
BsaI GGTCTC 1 cut(s) 289
BsaJI CCNNGG 6 cut(s) 155, 259, 390, 981, 999, 1182
BsaXI ACNNNNNCTCC 4 cut(s) 491, 521, 650, 680
Bsc4I CCNNNNNNNGG 4 cut(s) 90, 126, 173, 1060
Bse1I ACTGG 3 cut(s) 1151, 1454, 1468
Bse8I GATNNNNATC 1 cut(s) 1206
BseBI CCWGG 2 cut(s) 150, 1001
BseDI CCNNGG 6 cut(s) 155, 259, 390, 981, 999, 1182
BseGI GGATG 5 cut(s) 60, 105, 287, 609, 1074
BseJI GATNNNNATC 1 cut(s) 1206
BseLI CCNNNNNNNGG 4 cut(s) 90, 126, 173, 1060
BseNI ACTGG 3 cut(s) 1151, 1454, 1468
BseRI GAGGAG 4 cut(s) 89, 112, 512, 671
BseSI GKGCMC 2 cut(s) 1385, 1440
BseXI GCAGC 4 cut(s) 222, 276, 520, 849
BsgI GTGCAG 2 cut(s) 424, 536
Bsh1236I CGCG 1 cut(s) 1549
BshFI GGCC 4 cut(s) 153, 389, 395, 1004
BshNI GGYRCC 3 cut(s) 420, 1017, 1380
BsiHKAI GWGCWC 3 cut(s) 1053, 1440, 1458
BsiSI CCGG 1 cut(s) 1022
BslFI GGGAC 3 cut(s) 79, 505, 1350
BslI CCNNNNNNNGG 4 cut(s) 90, 126, 173, 1060
BsmAI GTCTC 2 cut(s) 289, 1043
BsmBI CGTCTC 1 cut(s) 1043
BsmFI GGGAC 3 cut(s) 79, 505, 1350
BsnI GGCC 4 cut(s) 153, 389, 395, 1004
Bso31I GGTCTC 1 cut(s) 289
Bsp119I TTCGAA 3 cut(s) 430, 780, 918
Bsp1286I GDGCHC 5 cut(s) 163, 1053, 1385, 1440, 1458
Bsp143I GATC 8 cut(s) 15, 556, 577, 637, 721, 796, 1072, 1261
Bsp19I CCATGG 2 cut(s) 390, 1182
BspACI CCGC 7 cut(s) 208, 300, 343, 536, 542, 978, 1552
BspANI GGCC 4 cut(s) 153, 389, 395, 1004
BspFNI CGCG 1 cut(s) 1549
BspHI TCATGA 1 cut(s) 799
BspLI GGNNCC 7 cut(s) 95, 241, 422, 964, 998, 1019, 1382
BspMAI CTGCAG 1 cut(s) 1335
BspMI ACCTGC 1 cut(s) 1338
BspOI GCTAGC 1 cut(s) 342
BspPI GGATC 3 cut(s) 804, 1080, 1256
BspT104I TTCGAA 3 cut(s) 430, 780, 918
BspT107I GGYRCC 3 cut(s) 420, 1017, 1380
BspTI CTTAAG 1 cut(s) 1138
BspTNI GGTCTC 1 cut(s) 289
BsrI ACTGG 3 cut(s) 1151, 1454, 1468
BssECI CCNNGG 6 cut(s) 155, 259, 390, 981, 999, 1182
BssMI GATC 8 cut(s) 15, 556, 577, 637, 721, 796, 1072, 1261
BssNAI GTATAC 1 cut(s) 910
BssNI GRCGYC 1 cut(s) 421
BssT1I CCWWGG 4 cut(s) 155, 259, 390, 1182
Bst1107I GTATAC 1 cut(s) 910
Bst2UI CCWGG 2 cut(s) 150, 1001
Bst4CI ACNGT 3 cut(s) 766, 1285, 1411
Bst6I CTCTTC 2 cut(s) 743, 1369
BstACI GRCGYC 1 cut(s) 421
BstAFI CTTAAG 1 cut(s) 1138
BstBAI YACGTR 1 cut(s) 597
BstBI TTCGAA 3 cut(s) 430, 780, 918
BstC8I GCNNGC 3 cut(s) 340, 1469, 1536
BstDEI CTNAG 1 cut(s) 560
BstDSI CCRYGG 3 cut(s) 390, 981, 1182
BstENI CCTNNNNNAGG 1 cut(s) 124
BstF5I GGATG 5 cut(s) 60, 105, 287, 609, 1074
BstFNI CGCG 1 cut(s) 1549
BstH2I RGCGCY 1 cut(s) 424
BstHHI GCGC 4 cut(s) 258, 423, 744, 1551
BstKTI GATC 8 cut(s) 18, 559, 580, 640, 724, 799, 1075, 1264
BstMAI GTCTC 2 cut(s) 289, 1043
BstMBI GATC 8 cut(s) 15, 556, 577, 637, 721, 796, 1072, 1261
BstMWI GCNNNNNNNGC 3 cut(s) 264, 539, 1540
BstNI CCWGG 2 cut(s) 150, 1001
BstNSI RCATGY 3 cut(s) 373, 916, 1471
BstSCI CCNGG 3 cut(s) 148, 999, 1020
BstSFI CTRYAG 2 cut(s) 1331, 1476
BstSLI GKGCMC 2 cut(s) 1385, 1440
BstUI CGCG 1 cut(s) 1549
BstV1I GCAGC 4 cut(s) 222, 276, 520, 849
BstV2I GAAGAC 2 cut(s) 67, 1225
BstX2I RGATCY 1 cut(s) 1261
BstXI CCANNNNNNTGG 2 cut(s) 280, 1272
BstYI RGATCY 1 cut(s) 1261
BstZ17I GTATAC 1 cut(s) 910
BsuRI GGCC 4 cut(s) 153, 389, 395, 1004
BtgI CCRYGG 3 cut(s) 390, 981, 1182
BtrI CACGTC 1 cut(s) 859
BtsCI GGATG 5 cut(s) 60, 105, 287, 609, 1074
BtsI GCAGTG 1 cut(s) 1438
BtsIMutI CAGTG 2 cut(s) 580, 1438
BveI ACCTGC 1 cut(s) 1338
Cac8I GCNNGC 3 cut(s) 340, 1469, 1536
CaiI CAGNNNCTG 2 cut(s) 523, 1283
CciI TCATGA 1 cut(s) 799
CfoI GCGC 4 cut(s) 258, 423, 744, 1551
Cfr13I GGNCC 7 cut(s) 93, 152, 239, 410, 996, 1157, 1248
Csp6I GTAC 3 cut(s) 235, 598, 827
CviQI GTAC 3 cut(s) 235, 598, 827
DdeI CTNAG 1 cut(s) 560
DinI GGCGCC 1 cut(s) 422
DpnI GATC 8 cut(s) 17, 558, 579, 639, 723, 798, 1074, 1263
DpnII GATC 8 cut(s) 15, 556, 577, 637, 721, 796, 1072, 1261
EaeI YGGCCR 1 cut(s) 1002
Eam1104I CTCTTC 2 cut(s) 743, 1369
EarI CTCTTC 2 cut(s) 743, 1369
EciI GGCGGA 1 cut(s) 967
Ecl136II GAGCTC 2 cut(s) 1051, 1456
Eco130I CCWWGG 4 cut(s) 155, 259, 390, 1182
Eco24I GRGCYC 3 cut(s) 163, 1053, 1458
Eco31I GGTCTC 1 cut(s) 289
Eco32I GATATC 1 cut(s) 1208
Eco47I GGWCC 6 cut(s) 93, 239, 410, 996, 1157, 1248
Eco53kI GAGCTC 2 cut(s) 1051, 1456
Eco57I CTGAAG 3 cut(s) 33, 545, 710
EcoICRI GAGCTC 2 cut(s) 1051, 1456
EcoNI CCTNNNNNAGG 1 cut(s) 124
EcoO109I RGGNCCY 1 cut(s) 152
EcoRI GAATTC 1 cut(s) 1292
EcoRII CCWGG 2 cut(s) 148, 999
EcoRV GATATC 1 cut(s) 1208
EcoT14I CCWWGG 4 cut(s) 155, 259, 390, 1182
EcoT38I GRGCYC 3 cut(s) 163, 1053, 1458
EgeI GGCGCC 1 cut(s) 422
EheI GGCGCC 1 cut(s) 422
ErhI CCWWGG 4 cut(s) 155, 259, 390, 1182
Esp3I CGTCTC 1 cut(s) 1043
FalI AAGNNNNNCTT 4 cut(s) 1121, 1153, 1490, 1522
FaqI GGGAC 3 cut(s) 79, 505, 1350
FauI CCCGC 1 cut(s) 535
FauNDI CATATG 1 cut(s) 186
FblI GTMKAC 3 cut(s) 768, 855, 909
Fnu4HI GCNGC 7 cut(s) 211, 265, 300, 343, 534, 863, 1552
FokI GGATG 5 cut(s) 47, 92, 274, 616, 1081
FriOI GRGCYC 3 cut(s) 163, 1053, 1458
Fsp4HI GCNGC 7 cut(s) 211, 265, 300, 343, 534, 863, 1552
FspBI CTAG 8 cut(s) 24, 156, 191, 224, 260, 339, 813, 1418
GlaI GCGC 4 cut(s) 257, 422, 743, 1550
GluI GCNGC 7 cut(s) 211, 265, 300, 343, 534, 863, 1552
GsuI CTGGAG 3 cut(s) 132, 200, 1037
HaeII RGCGCY 1 cut(s) 424
HaeIII GGCC 4 cut(s) 153, 389, 395, 1004
HapII CCGG 1 cut(s) 1022
HhaI GCGC 4 cut(s) 258, 423, 744, 1551
Hin1I GRCGYC 1 cut(s) 421
Hin6I GCGC 4 cut(s) 256, 421, 742, 1549
HinP1I GCGC 4 cut(s) 256, 421, 742, 1549
HincII GTYRAC 3 cut(s) 769, 1327, 1404
HindII GTYRAC 3 cut(s) 769, 1327, 1404
HinfI GANTC 4 cut(s) 43, 468, 591, 1230
HpaI GTTAAC 1 cut(s) 1404
HpaII CCGG 1 cut(s) 1022
HphI GGTGA 3 cut(s) 12, 557, 704
Hpy166II GTNNAC 6 cut(s) 769, 856, 910, 1327, 1404, 1438
Hpy188I TCNGA 3 cut(s) 52, 403, 1279
Hpy8I GTNNAC 6 cut(s) 769, 856, 910, 1327, 1404, 1438
HpyAV CCTTC 7 cut(s) 57, 94, 379, 471, 486, 520, 562
HpyCH4III ACNGT 3 cut(s) 766, 1285, 1411
HpyCH4IV ACGT 5 cut(s) 596, 858, 1036, 1191, 1323
HpyCH4V TGCA 5 cut(s) 441, 517, 1333, 1393, 1438
HpyF10VI GCNNNNNNNGC 3 cut(s) 264, 539, 1540
HpyF3I CTNAG 1 cut(s) 560
HpySE526I ACGT 5 cut(s) 596, 858, 1036, 1191, 1323
Hsp92I GRCGYC 1 cut(s) 421
HspAI GCGC 4 cut(s) 256, 421, 742, 1549
KasI GGCGCC 1 cut(s) 420
KspAI GTTAAC 1 cut(s) 1404
Kzo9I GATC 8 cut(s) 15, 556, 577, 637, 721, 796, 1072, 1261
LmnI GCTCC 5 cut(s) 487, 677, 1056, 1453, 1461
Lsp1109I GCAGC 4 cut(s) 222, 276, 520, 849
MaeI CTAG 8 cut(s) 24, 156, 191, 224, 260, 339, 813, 1418
MaeII ACGT 5 cut(s) 596, 858, 1036, 1191, 1323
MaeIII GTNAC 2 cut(s) 3, 1319
MalI GATC 8 cut(s) 17, 558, 579, 639, 723, 798, 1074, 1263
MboI GATC 8 cut(s) 15, 556, 577, 637, 721, 796, 1072, 1261
MboII GAAGA 8 cut(s) 67, 173, 716, 760, 1097, 1225, 1301, 1386
MflI RGATCY 1 cut(s) 1261
MhlI GDGCHC 5 cut(s) 163, 1053, 1385, 1440, 1458
MluCI AATT 3 cut(s) 753, 789, 1292
Mly113I GGCGCC 1 cut(s) 421
MlyI GAGTC 2 cut(s) 52, 1239
MmeI TCCRAC 1 cut(s) 1135
MseI TTAA 5 cut(s) 315, 872, 887, 1139, 1403
MslI CAYNNNNRTG 1 cut(s) 278
MspA1I CMGCKG 1 cut(s) 302
MspCI CTTAAG 1 cut(s) 1138
MspI CCGG 1 cut(s) 1022
MspR9I CCNGG 3 cut(s) 150, 1001, 1022
MvaI CCWGG 2 cut(s) 150, 1001
MvnI CGCG 1 cut(s) 1549
MwoI GCNNNNNNNGC 3 cut(s) 264, 539, 1540
NarI GGCGCC 1 cut(s) 421
NciI CCSGG 1 cut(s) 1022
NcoI CCATGG 2 cut(s) 390, 1182
NdeI CATATG 1 cut(s) 186
NdeII GATC 8 cut(s) 15, 556, 577, 637, 721, 796, 1072, 1261
NheI GCTAGC 1 cut(s) 338
NlaIV GGNNCC 7 cut(s) 95, 241, 422, 964, 998, 1019, 1382
NmuCI GTSAC 1 cut(s) 3
NspI RCATGY 3 cut(s) 373, 916, 1471
NspV TTCGAA 3 cut(s) 430, 780, 918
OliI CACNNNNGTG 1 cut(s) 278
PaeI GCATGC 1 cut(s) 1471
PagI TCATGA 1 cut(s) 799
PciI ACATGT 2 cut(s) 369, 912
PfeI GAWTC 2 cut(s) 468, 591
PflMI CCANNNNNTGG 2 cut(s) 173, 1060
PkrI GCNGC 7 cut(s) 212, 266, 301, 344, 535, 864, 1553
PleI GAGTC 2 cut(s) 51, 1238
PluTI GGCGCC 1 cut(s) 424
PpsI GAGTC 2 cut(s) 51, 1238
Ppu21I YACGTR 1 cut(s) 597
PscI ACATGT 2 cut(s) 369, 912
PshBI ATTAAT 1 cut(s) 872
Psp124BI GAGCTC 2 cut(s) 1053, 1458
Psp1406I AACGTT 1 cut(s) 1323
Psp6I CCWGG 2 cut(s) 148, 999
PspGI CCWGG 2 cut(s) 148, 999
PspN4I GGNNCC 7 cut(s) 95, 241, 422, 964, 998, 1019, 1382
PspPI GGNCC 7 cut(s) 93, 152, 239, 410, 996, 1157, 1248
PstI CTGCAG 1 cut(s) 1335
PstNI CAGNNNCTG 2 cut(s) 523, 1283
PsuI RGATCY 1 cut(s) 1261
RsaI GTAC 3 cut(s) 236, 599, 828
RsaNI GTAC 3 cut(s) 235, 598, 827
RseI CAYNNNNRTG 1 cut(s) 278
SacI GAGCTC 2 cut(s) 1053, 1458
SalI GTCGAC 1 cut(s) 767
SaqAI TTAA 5 cut(s) 315, 872, 887, 1139, 1403
SatI GCNGC 7 cut(s) 211, 265, 300, 343, 534, 863, 1552
Sau3AI GATC 8 cut(s) 15, 556, 577, 637, 721, 796, 1072, 1261
Sau96I GGNCC 7 cut(s) 93, 152, 239, 410, 996, 1157, 1248
SbfI CCTGCAGG 1 cut(s) 1335
SchI GAGTC 2 cut(s) 52, 1239
ScrFI CCNGG 3 cut(s) 150, 1001, 1022
SdaI CCTGCAGG 1 cut(s) 1335
SduI GDGCHC 5 cut(s) 163, 1053, 1385, 1440, 1458
SfcI CTRYAG 2 cut(s) 1331, 1476
SfoI GGCGCC 1 cut(s) 422
SfuI TTCGAA 3 cut(s) 430, 780, 918
SinI GGWCC 6 cut(s) 93, 239, 410, 996, 1157, 1248
SmiMI CAYNNNNRTG 1 cut(s) 278
SmlI CTYRAG 4 cut(s) 124, 1012, 1114, 1138
SmoI CTYRAG 4 cut(s) 124, 1012, 1114, 1138
SphI GCATGC 1 cut(s) 1471
Sse8387I CCTGCAGG 1 cut(s) 1335
Sse9I AATT 3 cut(s) 753, 789, 1292
SsiI CCGC 7 cut(s) 208, 300, 343, 536, 542, 978, 1552
SspDI GGCGCC 1 cut(s) 420
SspI AATATT 1 cut(s) 1243
SspMI CTAG 8 cut(s) 24, 156, 191, 224, 260, 339, 813, 1418
SstI GAGCTC 2 cut(s) 1053, 1458
StyD4I CCNGG 3 cut(s) 148, 999, 1020
StyI CCWWGG 4 cut(s) 155, 259, 390, 1182
TaaI ACNGT 3 cut(s) 766, 1285, 1411
TaiI ACGT 5 cut(s) 599, 861, 1039, 1194, 1326
TasI AATT 3 cut(s) 753, 789, 1292
TatI WGTACW 1 cut(s) 826
TauI GCSGC 3 cut(s) 302, 345, 1554
TfiI GAWTC 2 cut(s) 468, 591
Tru1I TTAA 5 cut(s) 315, 872, 887, 1139, 1403
Tru9I TTAA 5 cut(s) 315, 872, 887, 1139, 1403
TscAI CASTG 2 cut(s) 580, 1445
TseFI GTSAC 1 cut(s) 3
TseI GCWGC 4 cut(s) 210, 264, 533, 862
Tsp45I GTSAC 1 cut(s) 3
TspDTI ATGAA 7 cut(s) 173, 761, 816, 858, 1046, 1225, 1328
TspGWI ACGGA 2 cut(s) 252, 474
TspRI CASTG 2 cut(s) 580, 1445
Van91I CCANNNNNTGG 2 cut(s) 173, 1060
Vha464I CTTAAG 1 cut(s) 1138
VneI GTGCAC 1 cut(s) 1436
VpaK11BI GGWCC 6 cut(s) 93, 239, 410, 996, 1157, 1248
VspI ATTAAT 1 cut(s) 872
XagI CCTNNNNNAGG 1 cut(s) 124
XapI RAATTY 1 cut(s) 1292
XbaI TCTAGA 1 cut(s) 812
XceI RCATGY 3 cut(s) 373, 916, 1471
XmaJI CCTAGG 2 cut(s) 155, 259
XmiI GTMKAC 3 cut(s) 768, 855, 909
XspI CTAG 8 cut(s) 24, 156, 191, 224, 260, 339, 813, 1418
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.