Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr6

Orthogroup ID

OG0000282

Physical Location & Seq

Genomic Coordinates Forward (+)

41789237 .. 41791656

Gene Structure 2420 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw6G020990.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1512 bp

ATGAGTCCTTCAACAATAACGACCATCCTCCTGGTTCTCCTCACATGCCTTTGGTCACTCATCTCTGCCTCCTCAAAATCAAAACACAGAAAACTACCACCCGGCCCTTGGTCACTGCCAATAACGGGAAACCTCCATATGCTAGGCAACCTCCCCCATCGAAGCCTCCAACAATTGGCCAAAAAATATGGACACATCATGTCCATTCGTCTAGGCAATGTTTCTACCATAGTAGTCTCCTCCCCACAAGCCGCAGAGCTATTCCTCAAAACTCATGACACTAATTTCGCCAGCCGGCCCAAAATCCAAGCCTCAGAGTACCTGTCCTACGGCACAAAGGGCATGGCCTTTTCCCAATACGGTCCCTATTGGCGCCATATGAGGAAGCTCTGCTCGCTTCATCTCTTCTGTCCAGCAAAAATGGAGGCTTTCAGGCCGCTGCGAAAGGAGGAACTGGGAGTGCTGGTAAGAAAGCTGAAGCGAGCTGCAGAGGAAGGTGGAGTCGTGGATGTTAGTGAGAAGATTGGTGTGATGAATGAGGACATAACGTATAGGATGGTGTTGGGCTGTAAAAGGGATGATAAGTTTGATTTGAAAGCCATTGTTAAGGAAGCGTTTTTCTTGACTGGGGCTTTCAATATATCTGATTTTGTTCCTTCCCTTGCTGCACTTGATATTCAGGGATTGACCAAGCGCTTAAAGAAAGTTAGCAAGACGATCGATCAACTCCTGGAGAAGATAATAGACGAGCACGAACAAGTTGCCAACACTAGGAGTGGGAAACAAGGCCATGAGGACTTTGTAGACGTGTTGCTTTCGTTAACGAACCAACGGTTGAACCCGAATGATGAGCAAGTACACTTTCTTGATCGAACAAATGTGAAAGCCATCTTACTAGACATGATCGCGGCGGCTTTTGATACTTCGGCCGCATCGATTGTTTGGAGCCTTGCCGAGCTCTTGAGGCATCCAAGGATCATGAAGAATCTCCAAGAAGAGCTCCAAAGTGTGGTTGGTATGGACCGAATGGTGGAAGAATCTGATTTGCCCAAGCTGGATTACTTGAGTATGGTGGTGAAGGAGAGCTTCAGACTACACCCAGTTGCACCGTTACTAATCCCACATGAATCCATCGAGGACATTACAATCGATGGATATGACATTCCCAAGAAGTCGCGGATCATAGTAAACATCTGGAGCATTGGAAGAGATCCTAATGTTTGGTCCGAAAATGTTGAAGAATTTTATCCTGAAAGGTTCATGAACAGCAATATAGACCTCCGGGGACATGACTTTCAGCTCATCCCATTTGGGTCTGGGCGCAGAGGGTGTCCGGGTATGCAATTAGGACTAACCACAGTTCGGCTAGTTTTGGCACAGCTGGTGCACTGCTTTAACTGGGAGCTCCCAAATGGCTTTGTACCTCAAGACTTGGACATGTCTGAGGATTTCGGCTTATCACTGTCAAAAGCCAAACACTTGCTTGCTAAGCCAACGTACCGTCTTATGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004497 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0008150 GO:0008152 GO:0009058 GO:0012505 GO:0016020 GO:0016491 GO:0016705 GO:0016709 GO:0019748 GO:0031090 GO:0031984 GO:0042175 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0044550 GO:0055114 GO:0098827

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

p450

Protein Analysis

503

Amino Acids

56.86

Weight (kDa)

8.55

Isoelectric Point (pI)

40.11

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
p450	PF00067	33 - 491	4.2e-104	Cytochrome P450

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000282)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_5g12350 FvH4_5g12380 FvH4_5g12392 FvH4_5g12400 FvH4_5g12430
malus_domestica	MD06G1213300.v1.1 MD06G1213500.v1.1 MD06G1213700.v1.1 MD06G1213800.v1.1 MD06G1213900.v1.1 MD06G1214000.v1.1 MD06G1214400.v1.1 MD14G1224300.v1.1 MD14G1224400.v1.1 MD14G1224600.v1.1 MD14G1224700.v1.1 MD14G1224800.v1.1 MD14G1224900.v1.1 MD14G1225100.v1.1 MD14G1225400.v1.1
prunus_persica	Prupe.5G219700_v2.0.a1 Prupe.5G219800_v2.0.a1 Prupe.5G219900_v2.0.a1 Prupe.8G078500_v2.0.a1
pyrus_communis	pycom06g19100 pycom14g18590 pycom14g18600 pycom14g18610 pycom14g18660
rosa_chinensis	RchiOBHm_Chr0c12g0499591 RchiOBHm_Chr2g0140591 RchiOBHm_Chr2g0140631 RchiOBHm_Chr2g0140641 RchiOBHm_Chr2g0140651 RchiOBHm_Chr4g0417991 RchiOBHm_Chr4g0418001 RchiOBHm_Chr4g0418101 RchiOBHm_Chr4g0418111 RchiOBHm_Chr4g0418121 RchiOBHm_Chr6g0280191 RchiOBHm_Chr6g0280241 RchiOBHm_Chr6g0280251 RchiOBHm_Chr7g0185031 RchiOBHm_Chr7g0185051 RchiOBHm_Chr7g0185081 RchiOBHm_Chr7g0185091 RchiOBHm_Chr7g0185111 RchiOBHm_Chr7g0185131 RchiOBHm_Chr7g0185151 RchiOBHm_Chr7g0185161 RchiOBHm_Chr7g0185191 RchiOBHm_Chr7g0222431
rosa_laevigata	RLG00000002061 RLG00000004934 RLG00000004935 RLG00000004937 RLG00000013071 RLG00000013077 RLG00000019621 RLG00000019881 RLG00000019884 RLG00000019887 RLG00000019888 RLG00000028839
rosa_multiflora	Rmu_co8014370.1_g000001 Rmu_co8334683.1_g000001 Rmu_co8440383.1_g000001 Rmu_sc0002826.1_g000008 Rmu_sc0002826.1_g000009 Rmu_sc0002826.1_g000030 Rmu_sc0004483.1_g000005 Rmu_sc0004638.1_g000003 Rmu_sc0004638.1_g000004 Rmu_sc0005756.1_g000003 Rmu_sc0006493.1_g000023 Rmu_sc0006493.1_g000024 Rmu_sc0007137.1_g000017 Rmu_sc0008496.1_g000022 Rmu_sc0008769.1_g000003 Rmu_sc0013612.1_g000026 Rmu_sc0013864.1_g000001 Rmu_sc0013864.1_g000007 Rmu_sc0013864.1_g000008 Rmu_sc0030128.1_g000001 Rmu_sc0041660.1_g000001 Rmu_ssc0000289.1_g000001
rosa_roxburghii	Rroxscaffold_2G00104640 Rroxscaffold_3G00253970 Rroxscaffold_3G00269170 Rroxscaffold_3G00269200 Rroxscaffold_3G00269210 Rroxscaffold_3G00269220 Rroxscaffold_3G00269230 Rroxscaffold_7G00188320
rosa_rugosa	Rorug01G0052300 Rorug05G0212000 Rorug06G0130700 Rorug06G0131100 Rorug06G0466200 Rorug06G0466300 Rorug06G0466400 Rorug06G0466500 Rorug06G0466700 Rorug07G0208500 Rorug07G0294600
rosa_samantha	Rh4AG137600 Rh6DG237300 Rh6DG237800 Rh7AG076200 Rh7AG076300 Rh7AG076500 Rh7AG076600 Rh7AG350700 Rh7BG070800 Rh7BG070900 Rh7BG071000 Rh7BG071100
rosa_wichuraiana	Rw6G020990 Rw6G021010 Rw7G005900 Rw7G005910 Rw7G005920 Rw7G005940 Rw7G005950 Rw7G005970 Rw7G029600

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	372
AccB7I	CCANNNNNTGG	2 cut(s)	175, 1009
AccI	GTMKAC	1 cut(s)	804
AccII	CGCG	2 cut(s)	908, 1177
AciI	CCGC	6 cut(s)	252, 437, 908, 911, 930, 1177
AclWI	GGATC	3 cut(s)	983, 1187, 1205
AcoI	YGGCCR	2 cut(s)	177, 927
AcsI	RAATTY	1 cut(s)	1241
AcuI	CTGAAG	2 cut(s)	497, 1072
AcyI	GRCGYC	1 cut(s)	373
AfaI	GTAC	4 cut(s)	320, 858, 1422, 1499
AfeI	AGCGCT	1 cut(s)	695
AfiI	CCNNNNNNNGG	7 cut(s)	108, 125, 175, 771, 1009, 1030, 1362
AflIII	ACRYGT	2 cut(s)	807, 1437
AgsI	TTSAA	5 cut(s)	12, 595, 637, 838, 1238
AjiI	CACGTC	1 cut(s)	808
AjnI	CCWGG	2 cut(s)	30, 729
Alw21I	GWGCWC	5 cut(s)	753, 960, 1002, 1389, 1407
Alw26I	GTCTC	1 cut(s)	241
Alw44I	GTGCAC	1 cut(s)	1385
AlwI	GGATC	3 cut(s)	983, 1187, 1205
Aor51HI	AGCGCT	1 cut(s)	695
AoxI	GGCC	7 cut(s)	103, 177, 296, 345, 434, 787, 927
ApaLI	GTGCAC	1 cut(s)	1385
ApeKI	GCWGC	3 cut(s)	439, 485, 665
ApoI	RAATTY	1 cut(s)	1241
ArsI	GACNNNNNNTTYG	2 cut(s)	269, 301
AspLEI	GCGC	3 cut(s)	375, 696, 1323
AspS9I	GGNCC	5 cut(s)	104, 297, 362, 1021, 1224
AsuC2I	CCSGG	3 cut(s)	102, 1283, 1335
AsuHPI	GGTGA	1 cut(s)	1087
AvaII	GGWCC	3 cut(s)	362, 1021, 1224
BaeGI	GKGCMC	1 cut(s)	1389
BalI	TGGCCA	1 cut(s)	179
BanI	GGYRCC	1 cut(s)	372
BanII	GRGCYC	3 cut(s)	960, 1002, 1407
Bbv12I	GWGCWC	5 cut(s)	753, 960, 1002, 1389, 1407
BbvI	GCAGC	3 cut(s)	426, 472, 652
BccI	CCATC	6 cut(s)	32, 165, 550, 896, 1139, 1145
BceAI	ACGGC	1 cut(s)	346
BcgI	CGANNNNNNTGC	4 cut(s)	700, 734, 743, 777
BciT130I	CCWGG	2 cut(s)	32, 731
BcnI	CCSGG	3 cut(s)	102, 1283, 1335
BcoDI	GTCTC	1 cut(s)	241
BfaI	CTAG	5 cut(s)	143, 212, 771, 896, 1367
BfmI	CTRYAG	1 cut(s)	486
BfoI	RGCGCY	2 cut(s)	376, 697
BisI	GCNGC	8 cut(s)	252, 437, 440, 486, 666, 909, 912, 930
BlpI	GCTNAGC	1 cut(s)	1488
BlsI	GCNGC	8 cut(s)	253, 438, 441, 487, 667, 910, 913, 931
Bme1390I	CCNGG	5 cut(s)	32, 102, 731, 1283, 1335
Bme18I	GGWCC	3 cut(s)	362, 1021, 1224
BmgBI	CACGTC	1 cut(s)	808
BmgT120I	GGNCC	5 cut(s)	104, 297, 362, 1021, 1224
BmiI	GGNNCC	3 cut(s)	364, 374, 947
BmrFI	CCNGG	5 cut(s)	32, 102, 731, 1283, 1335
BmrI	ACTGGG	4 cut(s)	464, 636, 1094, 1408
BmsI	GCATC	2 cut(s)	941, 976
BmuI	ACTGGG	4 cut(s)	464, 636, 1094, 1408
BpmI	CTGGAG	2 cut(s)	752, 1216
Bpu1102I	GCTNAGC	1 cut(s)	1488
BpuEI	CTTGAG	3 cut(s)	982, 1084, 1410
BpuMI	CCSGG	3 cut(s)	102, 1283, 1335
Bsa29I	ATCGAT	3 cut(s)	720, 935, 1149
BsaHI	GRCGYC	1 cut(s)	373
BsaJI	CCNNGG	3 cut(s)	107, 971, 1282
BsaXI	ACNNNNNCTCC	2 cut(s)	450, 480
Bsc4I	CCNNNNNNNGG	7 cut(s)	108, 125, 175, 771, 1009, 1030, 1362
Bse118I	RCCGGY	1 cut(s)	294
Bse1I	ACTGG	4 cut(s)	459, 631, 1100, 1403
Bse3DI	GCAATG	1 cut(s)	223
BseBI	CCWGG	2 cut(s)	32, 731
BseCI	ATCGAT	3 cut(s)	720, 935, 1149
BseDI	CCNNGG	3 cut(s)	107, 971, 1282
BseGI	GGATG	6 cut(s)	24, 514, 561, 583, 967, 1302
BseLI	CCNNNNNNNGG	7 cut(s)	108, 125, 175, 771, 1009, 1030, 1362
BseMI	GCAATG	1 cut(s)	223
BseMII	CTCAG	2 cut(s)	327, 1434
BseNI	ACTGG	4 cut(s)	459, 631, 1100, 1403
BseRI	GAGGAG	3 cut(s)	29, 61, 229
BseSI	GKGCMC	1 cut(s)	1389
BseX3I	CGGCCG	1 cut(s)	927
BseXI	GCAGC	3 cut(s)	426, 472, 652
BsgI	GTGCAG	1 cut(s)	651
Bsh1236I	CGCG	2 cut(s)	908, 1177
Bsh1285I	CGRYCG	2 cut(s)	720, 930
BshFI	GGCC	7 cut(s)	105, 179, 298, 347, 436, 789, 929
BshNI	GGYRCC	1 cut(s)	372
BshVI	ATCGAT	3 cut(s)	720, 935, 1149
BsiEI	CGRYCG	2 cut(s)	720, 930
BsiHKAI	GWGCWC	5 cut(s)	753, 960, 1002, 1389, 1407
BsiSI	CCGG	4 cut(s)	102, 295, 1282, 1334
BslFI	GGGAC	2 cut(s)	348, 1299
BslI	CCNNNNNNNGG	7 cut(s)	108, 125, 175, 771, 1009, 1030, 1362
BsmAI	GTCTC	1 cut(s)	241
BsmFI	GGGAC	2 cut(s)	348, 1299
BsnI	GGCC	7 cut(s)	105, 179, 298, 347, 436, 789, 929
Bsp1286I	GDGCHC	5 cut(s)	753, 960, 1002, 1389, 1407
Bsp143I	GATC	7 cut(s)	717, 721, 868, 903, 975, 1179, 1210
Bsp1720I	GCTNAGC	1 cut(s)	1488
BspACI	CCGC	6 cut(s)	252, 437, 908, 911, 930, 1177
BspANI	GGCC	7 cut(s)	105, 179, 298, 347, 436, 789, 929
BspCNI	CTCAG	2 cut(s)	326, 1435
BspDI	ATCGAT	3 cut(s)	720, 935, 1149
BspFNI	CGCG	2 cut(s)	908, 1177
BspHI	TCATGA	3 cut(s)	274, 978, 1260
BspLI	GGNNCC	3 cut(s)	364, 374, 947
BspMAI	CTGCAG	1 cut(s)	490
BspPI	GGATC	3 cut(s)	983, 1187, 1205
BspQI	GCTCTTC	1 cut(s)	990
BspT107I	GGYRCC	1 cut(s)	372
BsrDI	GCAATG	1 cut(s)	223
BsrFI	RCCGGY	1 cut(s)	294
BsrI	ACTGG	4 cut(s)	459, 631, 1100, 1403
BssAI	RCCGGY	1 cut(s)	294
BssECI	CCNNGG	3 cut(s)	107, 971, 1282
BssMI	GATC	7 cut(s)	717, 721, 868, 903, 975, 1179, 1210
BssNI	GRCGYC	1 cut(s)	373
BssT1I	CCWWGG	2 cut(s)	107, 971
Bst2UI	CCWGG	2 cut(s)	32, 731
Bst4CI	ACNGT	6 cut(s)	362, 834, 1110, 1360, 1464, 1502
Bst6I	CTCTTC	3 cut(s)	410, 990, 1201
BstACI	GRCGYC	1 cut(s)	373
BstC8I	GCNNGC	5 cut(s)	292, 296, 395, 483, 1485
BstDEI	CTNAG	3 cut(s)	313, 1443, 1488
BstF5I	GGATG	6 cut(s)	24, 514, 561, 583, 967, 1302
BstFNI	CGCG	2 cut(s)	908, 1177
BstH2I	RGCGCY	2 cut(s)	376, 697
BstHHI	GCGC	3 cut(s)	375, 696, 1323
BstKTI	GATC	7 cut(s)	720, 724, 871, 906, 978, 1182, 1213
BstMAI	GTCTC	1 cut(s)	241
BstMBI	GATC	7 cut(s)	717, 721, 868, 903, 975, 1179, 1210
BstMCI	CGRYCG	2 cut(s)	720, 930
BstMWI	GCNNNNNNNGC	4 cut(s)	339, 394, 964, 1489
BstNI	CCWGG	2 cut(s)	32, 731
BstNSI	RCATGY	2 cut(s)	48, 1441
BstSCI	CCNGG	5 cut(s)	30, 100, 729, 1281, 1333
BstSFI	CTRYAG	1 cut(s)	486
BstSLI	GKGCMC	1 cut(s)	1389
BstUI	CGCG	2 cut(s)	908, 1177
BstV1I	GCAGC	3 cut(s)	426, 472, 652
BstX2I	RGATCY	1 cut(s)	1210
BstXI	CCANNNNNNTGG	1 cut(s)	31
BstYI	RGATCY	1 cut(s)	1210
BstZI	CGGCCG	1 cut(s)	927
Bsu15I	ATCGAT	3 cut(s)	720, 935, 1149
BsuRI	GGCC	7 cut(s)	105, 179, 298, 347, 436, 789, 929
BsuTUI	ATCGAT	3 cut(s)	720, 935, 1149
BtrI	CACGTC	1 cut(s)	808
BtsCI	GGATG	6 cut(s)	24, 514, 561, 583, 967, 1302
BtsI	GCAGTG	2 cut(s)	113, 1387
BtsIMutI	CAGTG	3 cut(s)	113, 1387, 1460
Cac8I	GCNNGC	5 cut(s)	292, 296, 395, 483, 1485
CciI	TCATGA	3 cut(s)	274, 978, 1260
CfoI	GCGC	3 cut(s)	375, 696, 1323
Cfr10I	RCCGGY	1 cut(s)	294
Cfr13I	GGNCC	5 cut(s)	104, 297, 362, 1021, 1224
ClaI	ATCGAT	3 cut(s)	720, 935, 1149
Csp6I	GTAC	4 cut(s)	319, 857, 1421, 1498
CviQI	GTAC	4 cut(s)	319, 857, 1421, 1498
DdeI	CTNAG	3 cut(s)	313, 1443, 1488
DinI	GGCGCC	1 cut(s)	374
DpnI	GATC	7 cut(s)	719, 723, 870, 905, 977, 1181, 1212
DpnII	GATC	7 cut(s)	717, 721, 868, 903, 975, 1179, 1210
EaeI	YGGCCR	2 cut(s)	177, 927
EagI	CGGCCG	1 cut(s)	927
Eam1104I	CTCTTC	3 cut(s)	410, 990, 1201
EarI	CTCTTC	3 cut(s)	410, 990, 1201
Ecl136II	GAGCTC	3 cut(s)	958, 1000, 1405
EclXI	CGGCCG	1 cut(s)	927
Eco130I	CCWWGG	2 cut(s)	107, 971
Eco24I	GRGCYC	3 cut(s)	960, 1002, 1407
Eco47I	GGWCC	3 cut(s)	362, 1021, 1224
Eco47III	AGCGCT	1 cut(s)	695
Eco52I	CGGCCG	1 cut(s)	927
Eco53kI	GAGCTC	3 cut(s)	958, 1000, 1405
Eco57I	CTGAAG	2 cut(s)	497, 1072
EcoICRI	GAGCTC	3 cut(s)	958, 1000, 1405
EcoRII	CCWGG	2 cut(s)	30, 729
EcoT14I	CCWWGG	2 cut(s)	107, 971
EcoT38I	GRGCYC	3 cut(s)	960, 1002, 1407
EgeI	GGCGCC	1 cut(s)	374
EheI	GGCGCC	1 cut(s)	374
ErhI	CCWWGG	2 cut(s)	107, 971
FalI	AAGNNNNNCTT	2 cut(s)	1070, 1102
FaqI	GGGAC	2 cut(s)	348, 1299
FauNDI	CATATG	2 cut(s)	138, 378
FblI	GTMKAC	1 cut(s)	804
Fnu4HI	GCNGC	8 cut(s)	252, 437, 440, 486, 666, 909, 912, 930
FokI	GGATG	6 cut(s)	11, 521, 568, 590, 954, 1289
FriOI	GRGCYC	3 cut(s)	960, 1002, 1407
Fsp4HI	GCNGC	8 cut(s)	252, 437, 440, 486, 666, 909, 912, 930
FspBI	CTAG	5 cut(s)	143, 212, 771, 896, 1367
GlaI	GCGC	3 cut(s)	374, 695, 1322
GluI	GCNGC	8 cut(s)	252, 437, 440, 486, 666, 909, 912, 930
GsuI	CTGGAG	2 cut(s)	752, 1216
HaeII	RGCGCY	2 cut(s)	376, 697
HaeIII	GGCC	7 cut(s)	105, 179, 298, 347, 436, 789, 929
HapII	CCGG	4 cut(s)	102, 295, 1282, 1334
HhaI	GCGC	3 cut(s)	375, 696, 1323
Hin1I	GRCGYC	1 cut(s)	373
Hin6I	GCGC	3 cut(s)	373, 694, 1321
HinP1I	GCGC	3 cut(s)	373, 694, 1321
HincII	GTYRAC	1 cut(s)	822
HindII	GTYRAC	1 cut(s)	822
HinfI	GANTC	5 cut(s)	4, 501, 985, 1037, 1127
HpaI	GTTAAC	1 cut(s)	822
HpaII	CCGG	4 cut(s)	102, 295, 1282, 1334
HphI	GGTGA	1 cut(s)	1087
Hpy166II	GTNNAC	5 cut(s)	805, 822, 859, 1189, 1387
Hpy188I	TCNGA	6 cut(s)	316, 646, 1042, 1091, 1228, 1444
Hpy188III	TCNNGA	9 cut(s)	275, 622, 866, 961, 979, 1195, 1250, 1261, 1427
Hpy8I	GTNNAC	5 cut(s)	805, 822, 859, 1189, 1387
HpyAV	CCTTC	4 cut(s)	18, 488, 666, 1072
HpyCH4III	ACNGT	6 cut(s)	362, 834, 1110, 1360, 1464, 1502
HpyCH4IV	ACGT	3 cut(s)	548, 807, 1496
HpyCH4V	TGCA	5 cut(s)	488, 668, 1106, 1342, 1387
HpyF10VI	GCNNNNNNNGC	4 cut(s)	339, 394, 964, 1489
HpyF3I	CTNAG	3 cut(s)	313, 1443, 1488
HpySE526I	ACGT	3 cut(s)	548, 807, 1496
Hsp92I	GRCGYC	1 cut(s)	373
HspAI	GCGC	3 cut(s)	373, 694, 1321
KasI	GGCGCC	1 cut(s)	372
KroI	GCCGGC	1 cut(s)	294
KroNI	GCCGGC	1 cut(s)	296
KspAI	GTTAAC	1 cut(s)	822
Kzo9I	GATC	7 cut(s)	717, 721, 868, 903, 975, 1179, 1210
LguI	GCTCTTC	1 cut(s)	990
LmnI	GCTCC	5 cut(s)	945, 1005, 1197, 1402, 1410
Lsp1109I	GCAGC	3 cut(s)	426, 472, 652
LweI	GCATC	2 cut(s)	941, 976
MaeI	CTAG	5 cut(s)	143, 212, 771, 896, 1367
MaeII	ACGT	3 cut(s)	548, 807, 1496
MaeIII	GTNAC	3 cut(s)	54, 111, 1110
MalI	GATC	7 cut(s)	719, 723, 870, 905, 977, 1181, 1212
MboI	GATC	7 cut(s)	717, 721, 868, 903, 975, 1179, 1210
MboII	GAAGA	8 cut(s)	397, 532, 748, 994, 1007, 1046, 1218, 1250
MfeI	CAATTG	1 cut(s)	173
MflI	RGATCY	1 cut(s)	1210
MhlI	GDGCHC	5 cut(s)	753, 960, 1002, 1389, 1407
MlsI	TGGCCA	1 cut(s)	179
MluCI	AATT	4 cut(s)	173, 283, 1241, 1343
MluNI	TGGCCA	1 cut(s)	179
Mly113I	GGCGCC	1 cut(s)	373
MlyI	GAGTC	2 cut(s)	13, 510
MmeI	TCCRAC	1 cut(s)	193
Mox20I	TGGCCA	1 cut(s)	179
MroNI	GCCGGC	1 cut(s)	294
MscI	TGGCCA	1 cut(s)	179
MseI	TTAA	4 cut(s)	606, 698, 821, 1395
Msp20I	TGGCCA	1 cut(s)	179
MspA1I	CMGCKG	2 cut(s)	439, 1381
MspI	CCGG	4 cut(s)	102, 295, 1282, 1334
MspR9I	CCNGG	5 cut(s)	32, 102, 731, 1283, 1335
MunI	CAATTG	1 cut(s)	173
MvaI	CCWGG	2 cut(s)	32, 731
MvnI	CGCG	2 cut(s)	908, 1177
MwoI	GCNNNNNNNGC	4 cut(s)	339, 394, 964, 1489
NaeI	GCCGGC	1 cut(s)	296
NarI	GGCGCC	1 cut(s)	373
NciI	CCSGG	3 cut(s)	102, 1283, 1335
NdeI	CATATG	2 cut(s)	138, 378
NdeII	GATC	7 cut(s)	717, 721, 868, 903, 975, 1179, 1210
NgoMIV	GCCGGC	1 cut(s)	294
NlaIV	GGNNCC	3 cut(s)	364, 374, 947
NmeAIII	GCCGAG	1 cut(s)	979
NmuCI	GTSAC	2 cut(s)	54, 111
NspI	RCATGY	2 cut(s)	48, 1441
PagI	TCATGA	3 cut(s)	274, 978, 1260
PciI	ACATGT	1 cut(s)	1437
PciSI	GCTCTTC	1 cut(s)	990
PcsI	WCGNNNNNNNCGW	1 cut(s)	932
PdiI	GCCGGC	1 cut(s)	296
PfeI	GAWTC	3 cut(s)	985, 1037, 1127
PflMI	CCANNNNNTGG	2 cut(s)	175, 1009
PfoI	TCCNGGA	1 cut(s)	729
PkrI	GCNGC	8 cut(s)	253, 438, 441, 487, 667, 910, 913, 931
Ple19I	CGATCG	1 cut(s)	720
PleI	GAGTC	2 cut(s)	12, 509
PluTI	GGCGCC	1 cut(s)	376
PpsI	GAGTC	2 cut(s)	12, 509
PscI	ACATGT	1 cut(s)	1437
Psp124BI	GAGCTC	3 cut(s)	960, 1002, 1407
Psp6I	CCWGG	2 cut(s)	30, 729
PspGI	CCWGG	2 cut(s)	30, 729
PspN4I	GGNNCC	3 cut(s)	364, 374, 947
PspPI	GGNCC	5 cut(s)	104, 297, 362, 1021, 1224
PstI	CTGCAG	1 cut(s)	490
PsuI	RGATCY	1 cut(s)	1210
PvuI	CGATCG	1 cut(s)	720
PvuII	CAGCTG	1 cut(s)	1381
RsaI	GTAC	4 cut(s)	320, 858, 1422, 1499
RsaNI	GTAC	4 cut(s)	319, 857, 1421, 1498
SacI	GAGCTC	3 cut(s)	960, 1002, 1407
SapI	GCTCTTC	1 cut(s)	990
SaqAI	TTAA	4 cut(s)	606, 698, 821, 1395
SatI	GCNGC	8 cut(s)	252, 437, 440, 486, 666, 909, 912, 930
Sau3AI	GATC	7 cut(s)	717, 721, 868, 903, 975, 1179, 1210
Sau96I	GGNCC	5 cut(s)	104, 297, 362, 1021, 1224
SchI	GAGTC	2 cut(s)	13, 510
ScrFI	CCNGG	5 cut(s)	32, 102, 731, 1283, 1335
SduI	GDGCHC	5 cut(s)	753, 960, 1002, 1389, 1407
SfaNI	GCATC	2 cut(s)	941, 976
SfcI	CTRYAG	1 cut(s)	486
SfoI	GGCGCC	1 cut(s)	374
SinI	GGWCC	3 cut(s)	362, 1021, 1224
SmlI	CTYRAG	3 cut(s)	961, 1063, 1425
SmoI	CTYRAG	3 cut(s)	961, 1063, 1425
Sse9I	AATT	4 cut(s)	173, 283, 1241, 1343
SsiI	CCGC	6 cut(s)	252, 437, 908, 911, 930, 1177
SspDI	GGCGCC	1 cut(s)	372
SspMI	CTAG	5 cut(s)	143, 212, 771, 896, 1367
SstI	GAGCTC	3 cut(s)	960, 1002, 1407
StyD4I	CCNGG	5 cut(s)	30, 100, 729, 1281, 1333
StyI	CCWWGG	2 cut(s)	107, 971
TaaI	ACNGT	6 cut(s)	362, 834, 1110, 1360, 1464, 1502
TaiI	ACGT	3 cut(s)	551, 810, 1499
TaqI	TCGA	6 cut(s)	160, 720, 871, 935, 1134, 1149
TaqII	GACCGA	1 cut(s)	1038
TasI	AATT	4 cut(s)	173, 283, 1241, 1343
TatI	WGTACW	1 cut(s)	856
TauI	GCSGC	5 cut(s)	254, 439, 911, 914, 932
TfiI	GAWTC	3 cut(s)	985, 1037, 1127
Tru1I	TTAA	4 cut(s)	606, 698, 821, 1395
Tru9I	TTAA	4 cut(s)	606, 698, 821, 1395
TscAI	CASTG	3 cut(s)	120, 1394, 1467
TseFI	GTSAC	2 cut(s)	54, 111
TseI	GCWGC	3 cut(s)	439, 485, 665
Tsp45I	GTSAC	2 cut(s)	54, 111
TspDTI	ATGAA	6 cut(s)	389, 548, 995, 1140, 1249, 1277
TspRI	CASTG	3 cut(s)	120, 1394, 1467
Van91I	CCANNNNNTGG	2 cut(s)	175, 1009
VneI	GTGCAC	1 cut(s)	1385
VpaK11BI	GGWCC	3 cut(s)	362, 1021, 1224
XapI	RAATTY	1 cut(s)	1241
XceI	RCATGY	2 cut(s)	48, 1441
XcmI	CCANNNNNNNNNTGG	2 cut(s)	105, 1010
XmiI	GTMKAC	1 cut(s)	804
XspI	CTAG	5 cut(s)	143, 212, 771, 896, 1367

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.