Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0000282

Physical Location & Seq

Genomic Coordinates Forward (+)

30526719 .. 30529710

Gene Structure 2992 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1224300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1521 bp

ATGAGTCCTTCAGAAATAACCATCCTAATCCTTGTCTTCCTTACCTTCTTATGGTCTCTTCTCCGCATCCTAATCAACGCCTCCTCAAGGCAAAGCCAAAAACTGCCTCCTGGCCCTATGGCTCTGCCAATCATTGGCAATCTTCATATGCTAGGCAACCTCCCACACCGCAGCCTCCAGAACCTAGCCAAAAAGTACGGACCCATAATGTCCATGCGCCTAGGAAGCAAAACCACCATAGTAGTCTCATCCCCCAAAGCCGCTGAGTTATTCCTTAAAACCCACGACACCATTTTCGCTAGCCGCCCCCAAGTCCAAGCCTCCAAGTACATGTCGTACGACACGAAGGCCATGGCCTTTTCTGAATATGGTCCATATTGGCGCCATGTTCGAAAGCTCTGCACGCTTCAACTTCTTTCTCCTTTGAGAATCGAGGCTTTTGCTCCGCTGAGAAGGGAGGAGGTAGGGTTGTTGGTGCAATCCCTAAAGGTAGCCGCAGAGGCGGGTGAGGTGGTAGATCTTAGTGAGAAAGTCGGTGAGCTTGTTGTGGGAATCACGTACAGGATGGTGTTGGGGAGAAAAAACAATGATATGTTTGATCTCAAGGGGATTATAGAGGAGGTCATGTTCTTGTCAGGAGCTTTCAACATTGGTGATTATGTGCCTTTCCTTATTCCACTTGATGTTCAGGGATTGACCAAGCGCATGAAGAGAATTAGCAAGACTGTGGACCAACTATTCGAAAAGATAATTCAGGATCATGAACAAGTTTCTAGAAGTGAGCAAGTACAAGGCAATCATCATAAGGACTTTGTAGACGTGCTGCTTTCATTAATCCACCAACCATTAAACCCCAACGATGAGGAAGTATACATGTTCGAACGAACAAATGCCAAAGCCATTTTACTCGATATGATTTCTGGTTCCTTTGACACTTCAGCCACGGCAATCGTCTGGACCCTGGCCGAACTCTTGAGGCACCCGGAAGTTATGAAACGTCTCCAAAAAGAGCTCCAAAGTGTGGTCGGGATGGATCGAATGGTTGAAGAAAGTGATTTGCCAAAGCTGGATTACTTGAGCATGGTGGTCAAGGAGAGCTTCAGGCTACACCCTGTTGGACCATTACTTATCCCTCATCAATCCATGGAGGACGTTATAGTTGATGGATATCACATACCCAAGAAATCAAGAGTCTTCATCAATATTTGGACCATTGGGAGAGATCCAAATGTATGGTCAGATAATGTGGAAGAATTCTACCCTGAAAGGTTTATGAATAGTAACGTTGACCTGCAAGGACATGACTTCCAGCTCATACCATTTGGGTCTGGTCGAAGAGGGTGCCCTGCTATGCAGTTAGGGTTGACCACAGTTCGTCTAGCTCTGGGAAACTTGGTGAACTGCTTCAACTGGGAGCTCCCAACTGGCATGTTGCCTAAAGACTTGGACATGACTGAAAAGTTCGGCTTATCGTTGTCGAAAGCCAAACACTTGCTTGCCATGCCGACGCACCGCCTATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004497 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0008150 GO:0008152 GO:0009058 GO:0012505 GO:0016020 GO:0016491 GO:0016705 GO:0016709 GO:0019748 GO:0031090 GO:0031984 GO:0042175 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0044550 GO:0055114 GO:0098827

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

p450

Protein Analysis

507

Amino Acids

57.33

Weight (kDa)

7.77

Isoelectric Point (pI)

50.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
p450	PF00067	36 - 496	2.9e-100	Cytochrome P450

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000282)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_5g12350 FvH4_5g12380 FvH4_5g12392 FvH4_5g12400 FvH4_5g12430
malus_domestica	MD06G1213300.v1.1 MD06G1213500.v1.1 MD06G1213700.v1.1 MD06G1213800.v1.1 MD06G1213900.v1.1 MD06G1214000.v1.1 MD06G1214400.v1.1 MD14G1224300.v1.1 MD14G1224400.v1.1 MD14G1224600.v1.1 MD14G1224700.v1.1 MD14G1224800.v1.1 MD14G1224900.v1.1 MD14G1225100.v1.1 MD14G1225400.v1.1
prunus_persica	Prupe.5G219700_v2.0.a1 Prupe.5G219800_v2.0.a1 Prupe.5G219900_v2.0.a1 Prupe.8G078500_v2.0.a1
pyrus_communis	pycom06g19100 pycom14g18590 pycom14g18600 pycom14g18610 pycom14g18660
rosa_chinensis	RchiOBHm_Chr0c12g0499591 RchiOBHm_Chr2g0140591 RchiOBHm_Chr2g0140631 RchiOBHm_Chr2g0140641 RchiOBHm_Chr2g0140651 RchiOBHm_Chr4g0417991 RchiOBHm_Chr4g0418001 RchiOBHm_Chr4g0418101 RchiOBHm_Chr4g0418111 RchiOBHm_Chr4g0418121 RchiOBHm_Chr6g0280191 RchiOBHm_Chr6g0280241 RchiOBHm_Chr6g0280251 RchiOBHm_Chr7g0185031 RchiOBHm_Chr7g0185051 RchiOBHm_Chr7g0185081 RchiOBHm_Chr7g0185091 RchiOBHm_Chr7g0185111 RchiOBHm_Chr7g0185131 RchiOBHm_Chr7g0185151 RchiOBHm_Chr7g0185161 RchiOBHm_Chr7g0185191 RchiOBHm_Chr7g0222431
rosa_laevigata	RLG00000002061 RLG00000004934 RLG00000004935 RLG00000004937 RLG00000013071 RLG00000013077 RLG00000019621 RLG00000019881 RLG00000019884 RLG00000019887 RLG00000019888 RLG00000028839
rosa_multiflora	Rmu_co8014370.1_g000001 Rmu_co8334683.1_g000001 Rmu_co8440383.1_g000001 Rmu_sc0002826.1_g000008 Rmu_sc0002826.1_g000009 Rmu_sc0002826.1_g000030 Rmu_sc0004483.1_g000005 Rmu_sc0004638.1_g000003 Rmu_sc0004638.1_g000004 Rmu_sc0005756.1_g000003 Rmu_sc0006493.1_g000023 Rmu_sc0006493.1_g000024 Rmu_sc0007137.1_g000017 Rmu_sc0008496.1_g000022 Rmu_sc0008769.1_g000003 Rmu_sc0013612.1_g000026 Rmu_sc0013864.1_g000001 Rmu_sc0013864.1_g000007 Rmu_sc0013864.1_g000008 Rmu_sc0030128.1_g000001 Rmu_sc0041660.1_g000001 Rmu_ssc0000289.1_g000001
rosa_roxburghii	Rroxscaffold_2G00104640 Rroxscaffold_3G00253970 Rroxscaffold_3G00269170 Rroxscaffold_3G00269200 Rroxscaffold_3G00269210 Rroxscaffold_3G00269220 Rroxscaffold_3G00269230 Rroxscaffold_7G00188320
rosa_rugosa	Rorug01G0052300 Rorug05G0212000 Rorug06G0130700 Rorug06G0131100 Rorug06G0466200 Rorug06G0466300 Rorug06G0466400 Rorug06G0466500 Rorug06G0466700 Rorug07G0208500 Rorug07G0294600
rosa_samantha	Rh4AG137600 Rh6DG237300 Rh6DG237800 Rh7AG076200 Rh7AG076300 Rh7AG076500 Rh7AG076600 Rh7AG350700 Rh7BG070800 Rh7BG070900 Rh7BG071000 Rh7BG071100
rosa_wichuraiana	Rw6G020990 Rw6G021010 Rw7G005900 Rw7G005910 Rw7G005920 Rw7G005940 Rw7G005950 Rw7G005970 Rw7G029600

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1299
AccB1I	GGYRCC	3 cut(s)	381, 978, 1341
AccB7I	CCANNNNNTGG	2 cut(s)	134, 1021
AccI	GTMKAC	2 cut(s)	816, 870
AciI	CCGC	8 cut(s)	64, 169, 261, 304, 446, 495, 503, 1513
AclI	AACGTT	1 cut(s)	1284
AclWI	GGATC	3 cut(s)	765, 1041, 1217
AcoI	YGGCCR	1 cut(s)	963
AcsI	RAATTY	1 cut(s)	1253
AcuI	CTGAAG	2 cut(s)	921, 1084
AcyI	GRCGYC	1 cut(s)	382
AfaI	GTAC	5 cut(s)	197, 329, 338, 560, 789
AfiI	CCNNNNNNNGG	4 cut(s)	51, 87, 134, 1021
AflIII	ACRYGT	2 cut(s)	330, 873
AgsI	TTSAA	4 cut(s)	410, 646, 1046, 1408
AjiI	CACGTC	1 cut(s)	820
AjnI	CCWGG	2 cut(s)	109, 960
AloI	GAACNNNNNNTCC	2 cut(s)	611, 643
AluBI	AGCT	9 cut(s)	397, 541, 641, 1012, 1066, 1098, 1312, 1382, 1417
AluI	AGCT	9 cut(s)	397, 541, 641, 1012, 1066, 1098, 1312, 1382, 1417
Alw21I	GWGCWC	2 cut(s)	1014, 1419
Alw26I	GTCTC	3 cut(s)	60, 250, 1004
AlwI	GGATC	3 cut(s)	765, 1041, 1217
AoxI	GGCC	4 cut(s)	112, 348, 354, 963
ApeKI	GCWGC	2 cut(s)	171, 823
ApoI	RAATTY	1 cut(s)	1253
ArsI	GACNNNNNNTTYG	6 cut(s)	278, 310, 722, 754, 1220, 1252
AseI	ATTAAT	1 cut(s)	833
Asp700I	GAANNNNTTC	1 cut(s)	1403
AspA2I	CCTAGG	1 cut(s)	220
AspLEI	GCGC	3 cut(s)	219, 384, 705
AspS9I	GGNCC	7 cut(s)	113, 200, 371, 730, 957, 1118, 1209
AsuC2I	CCSGG	1 cut(s)	983
AsuHPI	GGTGA	4 cut(s)	518, 548, 665, 1408
AsuII	TTCGAA	3 cut(s)	391, 741, 879
AsuNHI	GCTAGC	1 cut(s)	299
AvaII	GGWCC	6 cut(s)	200, 371, 730, 957, 1118, 1209
AvrII	CCTAGG	1 cut(s)	220
BaeGI	GKGCMC	1 cut(s)	1346
BanI	GGYRCC	3 cut(s)	381, 978, 1341
BanII	GRGCYC	2 cut(s)	1014, 1419
BbsI	GAAGAC	2 cut(s)	28, 1186
Bbv12I	GWGCWC	2 cut(s)	1014, 1419
BbvI	GCAGC	2 cut(s)	183, 810
BccI	CCATC	4 cut(s)	29, 559, 1024, 1157
BceAI	ACGGC	1 cut(s)	960
BcgI	CGANNNNNNTGC	6 cut(s)	381, 415, 422, 456, 1323, 1357
BciT130I	CCWGG	2 cut(s)	111, 962
BcnI	CCSGG	1 cut(s)	983
BcoDI	GTCTC	3 cut(s)	60, 250, 1004
BfaI	CTAG	6 cut(s)	152, 185, 221, 300, 774, 1379
BfoI	RGCGCY	1 cut(s)	385
BfuAI	ACCTGC	1 cut(s)	1299
BglI	GCCNNNNNGGC	1 cut(s)	500
BglII	AGATCT	1 cut(s)	517
BisI	GCNGC	5 cut(s)	172, 261, 304, 495, 824
BlnI	CCTAGG	1 cut(s)	220
BlsI	GCNGC	5 cut(s)	173, 262, 305, 496, 825
Bme1390I	CCNGG	3 cut(s)	111, 962, 983
Bme18I	GGWCC	6 cut(s)	200, 371, 730, 957, 1118, 1209
BmgBI	CACGTC	1 cut(s)	820
BmgT120I	GGNCC	7 cut(s)	113, 200, 371, 730, 957, 1118, 1209
BmiI	GGNNCC	6 cut(s)	202, 383, 925, 959, 980, 1343
BmrFI	CCNGG	3 cut(s)	111, 962, 983
BmrI	ACTGGG	1 cut(s)	1420
BmsI	GCATC	1 cut(s)	75
BmtI	GCTAGC	1 cut(s)	303
BmuI	ACTGGG	1 cut(s)	1420
BpiI	GAAGAC	2 cut(s)	28, 1186
BpmI	CTGGAG	1 cut(s)	161
Bpu14I	TTCGAA	3 cut(s)	391, 741, 879
BpuEI	CTTGAG	4 cut(s)	70, 587, 994, 1096
BpuMI	CCSGG	1 cut(s)	983
BsaAI	YACGTR	1 cut(s)	558
BsaBI	GATNNNNATC	1 cut(s)	1167
BsaHI	GRCGYC	1 cut(s)	382
BsaI	GGTCTC	1 cut(s)	60
BsaJI	CCNNGG	5 cut(s)	220, 351, 942, 960, 1143
BsaXI	ACNNNNNCTCC	4 cut(s)	452, 482, 611, 641
Bsc4I	CCNNNNNNNGG	4 cut(s)	51, 87, 134, 1021
Bse1I	ACTGG	2 cut(s)	1415, 1429
Bse8I	GATNNNNATC	1 cut(s)	1167
BseBI	CCWGG	2 cut(s)	111, 962
BseDI	CCNNGG	5 cut(s)	220, 351, 942, 960, 1143
BseGI	GGATG	5 cut(s)	21, 66, 248, 570, 1035
BseJI	GATNNNNATC	1 cut(s)	1167
BseLI	CCNNNNNNNGG	4 cut(s)	51, 87, 134, 1021
BseMII	CTCAG	2 cut(s)	255, 440
BseNI	ACTGG	2 cut(s)	1415, 1429
BseRI	GAGGAG	3 cut(s)	73, 473, 632
BseSI	GKGCMC	1 cut(s)	1346
BseXI	GCAGC	2 cut(s)	183, 810
BsgI	GTGCAG	1 cut(s)	385
BshFI	GGCC	4 cut(s)	114, 350, 356, 965
BshNI	GGYRCC	3 cut(s)	381, 978, 1341
BsiHKAI	GWGCWC	2 cut(s)	1014, 1419
BsiSI	CCGG	1 cut(s)	983
BsiWI	CGTACG	1 cut(s)	336
BslI	CCNNNNNNNGG	4 cut(s)	51, 87, 134, 1021
BsmAI	GTCTC	3 cut(s)	60, 250, 1004
BsmBI	CGTCTC	1 cut(s)	1004
BsnI	GGCC	4 cut(s)	114, 350, 356, 965
Bso31I	GGTCTC	1 cut(s)	60
Bsp119I	TTCGAA	3 cut(s)	391, 741, 879
Bsp1286I	GDGCHC	3 cut(s)	1014, 1346, 1419
Bsp143I	GATC	5 cut(s)	517, 598, 757, 1033, 1222
Bsp19I	CCATGG	2 cut(s)	351, 1143
BspACI	CCGC	8 cut(s)	64, 169, 261, 304, 446, 495, 503, 1513
BspANI	GGCC	4 cut(s)	114, 350, 356, 965
BspCNI	CTCAG	2 cut(s)	256, 441
BspHI	TCATGA	1 cut(s)	760
BspLI	GGNNCC	6 cut(s)	202, 383, 925, 959, 980, 1343
BspMI	ACCTGC	1 cut(s)	1299
BspOI	GCTAGC	1 cut(s)	303
BspPI	GGATC	3 cut(s)	765, 1041, 1217
BspT104I	TTCGAA	3 cut(s)	391, 741, 879
BspT107I	GGYRCC	3 cut(s)	381, 978, 1341
BspTNI	GGTCTC	1 cut(s)	60
BsrI	ACTGG	2 cut(s)	1415, 1429
BssECI	CCNNGG	5 cut(s)	220, 351, 942, 960, 1143
BssMI	GATC	5 cut(s)	517, 598, 757, 1033, 1222
BssNAI	GTATAC	1 cut(s)	871
BssNI	GRCGYC	1 cut(s)	382
BssT1I	CCWWGG	3 cut(s)	220, 351, 1143
Bst1107I	GTATAC	1 cut(s)	871
Bst2UI	CCWGG	2 cut(s)	111, 962
Bst4CI	ACNGT	2 cut(s)	727, 1372
Bst6I	CTCTTC	3 cut(s)	63, 704, 1330
BstACI	GRCGYC	1 cut(s)	382
BstBAI	YACGTR	1 cut(s)	558
BstBI	TTCGAA	3 cut(s)	391, 741, 879
BstC8I	GCNNGC	3 cut(s)	301, 404, 1497
BstDEI	CTNAG	3 cut(s)	264, 449, 521
BstDSI	CCRYGG	3 cut(s)	351, 942, 1143
BstENI	CCTNNNNNAGG	1 cut(s)	85
BstF5I	GGATG	5 cut(s)	21, 66, 248, 570, 1035
BstH2I	RGCGCY	1 cut(s)	385
BstHHI	GCGC	3 cut(s)	219, 384, 705
BstKTI	GATC	5 cut(s)	520, 601, 760, 1036, 1225
BstMAI	GTCTC	3 cut(s)	60, 250, 1004
BstMBI	GATC	5 cut(s)	517, 598, 757, 1033, 1222
BstMWI	GCNNNNNNNGC	4 cut(s)	225, 403, 500, 1501
BstNI	CCWGG	2 cut(s)	111, 962
BstNSI	RCATGY	3 cut(s)	334, 877, 1432
BstSCI	CCNGG	3 cut(s)	109, 960, 981
BstSLI	GKGCMC	1 cut(s)	1346
BstV1I	GCAGC	2 cut(s)	183, 810
BstV2I	GAAGAC	2 cut(s)	28, 1186
BstX2I	RGATCY	2 cut(s)	517, 1222
BstXI	CCANNNNNNTGG	1 cut(s)	1233
BstYI	RGATCY	2 cut(s)	517, 1222
BstZ17I	GTATAC	1 cut(s)	871
BsuRI	GGCC	4 cut(s)	114, 350, 356, 965
BtgI	CCRYGG	3 cut(s)	351, 942, 1143
BtrI	CACGTC	1 cut(s)	820
BtsCI	GGATG	5 cut(s)	21, 66, 248, 570, 1035
BveI	ACCTGC	1 cut(s)	1299
Cac8I	GCNNGC	3 cut(s)	301, 404, 1497
CciI	TCATGA	1 cut(s)	760
CfoI	GCGC	3 cut(s)	219, 384, 705
Cfr13I	GGNCC	7 cut(s)	113, 200, 371, 730, 957, 1118, 1209
CseI	GACGC	1 cut(s)	1516
Csp6I	GTAC	5 cut(s)	196, 328, 337, 559, 788
CviQI	GTAC	5 cut(s)	196, 328, 337, 559, 788
DdeI	CTNAG	3 cut(s)	264, 449, 521
DinI	GGCGCC	1 cut(s)	383
DpnI	GATC	5 cut(s)	519, 600, 759, 1035, 1224
DpnII	GATC	5 cut(s)	517, 598, 757, 1033, 1222
EaeI	YGGCCR	1 cut(s)	963
Eam1104I	CTCTTC	3 cut(s)	63, 704, 1330
EarI	CTCTTC	3 cut(s)	63, 704, 1330
Ecl136II	GAGCTC	2 cut(s)	1012, 1417
Eco130I	CCWWGG	3 cut(s)	220, 351, 1143
Eco24I	GRGCYC	2 cut(s)	1014, 1419
Eco31I	GGTCTC	1 cut(s)	60
Eco32I	GATATC	1 cut(s)	1169
Eco47I	GGWCC	6 cut(s)	200, 371, 730, 957, 1118, 1209
Eco53kI	GAGCTC	2 cut(s)	1012, 1417
Eco57I	CTGAAG	2 cut(s)	921, 1084
EcoICRI	GAGCTC	2 cut(s)	1012, 1417
EcoNI	CCTNNNNNAGG	1 cut(s)	85
EcoRI	GAATTC	1 cut(s)	1253
EcoRII	CCWGG	2 cut(s)	109, 960
EcoRV	GATATC	1 cut(s)	1169
EcoT14I	CCWWGG	3 cut(s)	220, 351, 1143
EcoT38I	GRGCYC	2 cut(s)	1014, 1419
EgeI	GGCGCC	1 cut(s)	383
EheI	GGCGCC	1 cut(s)	383
ErhI	CCWWGG	3 cut(s)	220, 351, 1143
Esp3I	CGTCTC	1 cut(s)	1004
FalI	AAGNNNNNCTT	4 cut(s)	1082, 1114, 1451, 1483
FauI	CCCGC	1 cut(s)	496
FauNDI	CATATG	1 cut(s)	147
FblI	GTMKAC	2 cut(s)	816, 870
Fnu4HI	GCNGC	5 cut(s)	172, 261, 304, 495, 824
FokI	GGATG	5 cut(s)	8, 53, 235, 577, 1042
FriOI	GRGCYC	2 cut(s)	1014, 1419
Fsp4HI	GCNGC	5 cut(s)	172, 261, 304, 495, 824
FspBI	CTAG	6 cut(s)	152, 185, 221, 300, 774, 1379
GlaI	GCGC	3 cut(s)	218, 383, 704
GluI	GCNGC	5 cut(s)	172, 261, 304, 495, 824
GsuI	CTGGAG	1 cut(s)	161
HaeII	RGCGCY	1 cut(s)	385
HaeIII	GGCC	4 cut(s)	114, 350, 356, 965
HapII	CCGG	1 cut(s)	983
HgaI	GACGC	1 cut(s)	1516
HhaI	GCGC	3 cut(s)	219, 384, 705
Hin1I	GRCGYC	1 cut(s)	382
Hin6I	GCGC	3 cut(s)	217, 382, 703
HinP1I	GCGC	3 cut(s)	217, 382, 703
HincII	GTYRAC	2 cut(s)	1288, 1365
HindII	GTYRAC	2 cut(s)	1288, 1365
HinfI	GANTC	4 cut(s)	4, 429, 552, 1191
HpaII	CCGG	1 cut(s)	983
HphI	GGTGA	4 cut(s)	518, 548, 665, 1408
Hpy166II	GTNNAC	6 cut(s)	730, 817, 871, 1288, 1365, 1399
Hpy188I	TCNGA	3 cut(s)	13, 364, 1240
Hpy188III	TCNNGA	9 cut(s)	178, 636, 755, 761, 774, 955, 973, 1027, 1188
Hpy8I	GTNNAC	6 cut(s)	730, 817, 871, 1288, 1365, 1399
Hpy99I	CGWCG	1 cut(s)	1510
HpyAV	CCTTC	4 cut(s)	18, 55, 340, 447
HpyCH4III	ACNGT	2 cut(s)	727, 1372
HpyCH4IV	ACGT	5 cut(s)	557, 819, 997, 1152, 1284
HpyCH4V	TGCA	4 cut(s)	402, 478, 1294, 1354
HpyF10VI	GCNNNNNNNGC	4 cut(s)	225, 403, 500, 1501
HpyF3I	CTNAG	3 cut(s)	264, 449, 521
HpySE526I	ACGT	5 cut(s)	557, 819, 997, 1152, 1284
Hsp92I	GRCGYC	1 cut(s)	382
HspAI	GCGC	3 cut(s)	217, 382, 703
KasI	GGCGCC	1 cut(s)	381
Kzo9I	GATC	5 cut(s)	517, 598, 757, 1033, 1222
LmnI	GCTCC	5 cut(s)	448, 638, 1017, 1414, 1422
Lsp1109I	GCAGC	2 cut(s)	183, 810
LweI	GCATC	1 cut(s)	75
MaeI	CTAG	6 cut(s)	152, 185, 221, 300, 774, 1379
MaeII	ACGT	5 cut(s)	557, 819, 997, 1152, 1284
MaeIII	GTNAC	1 cut(s)	1280
MalI	GATC	5 cut(s)	519, 600, 759, 1035, 1224
MboI	GATC	5 cut(s)	517, 598, 757, 1033, 1222
MboII	GAAGA	8 cut(s)	28, 50, 134, 721, 1058, 1186, 1262, 1347
MflI	RGATCY	2 cut(s)	517, 1222
MhlI	GDGCHC	3 cut(s)	1014, 1346, 1419
MluCI	AATT	3 cut(s)	714, 750, 1253
Mly113I	GGCGCC	1 cut(s)	382
MlyI	GAGTC	2 cut(s)	13, 1200
MmeI	TCCRAC	1 cut(s)	1096
MroXI	GAANNNNTTC	1 cut(s)	1403
MseI	TTAA	3 cut(s)	276, 833, 848
MspA1I	CMGCKG	2 cut(s)	263, 448
MspI	CCGG	1 cut(s)	983
MspR9I	CCNGG	3 cut(s)	111, 962, 983
MvaI	CCWGG	2 cut(s)	111, 962
MwoI	GCNNNNNNNGC	4 cut(s)	225, 403, 500, 1501
NarI	GGCGCC	1 cut(s)	382
NciI	CCSGG	1 cut(s)	983
NcoI	CCATGG	2 cut(s)	351, 1143
NdeI	CATATG	1 cut(s)	147
NdeII	GATC	5 cut(s)	517, 598, 757, 1033, 1222
NheI	GCTAGC	1 cut(s)	299
NlaIV	GGNNCC	6 cut(s)	202, 383, 925, 959, 980, 1343
NspI	RCATGY	3 cut(s)	334, 877, 1432
NspV	TTCGAA	3 cut(s)	391, 741, 879
PagI	TCATGA	1 cut(s)	760
PciI	ACATGT	2 cut(s)	330, 873
PcsI	WCGNNNNNNNCGW	1 cut(s)	341
PdmI	GAANNNNTTC	1 cut(s)	1403
PfeI	GAWTC	2 cut(s)	429, 552
Pfl23II	CGTACG	1 cut(s)	336
PflMI	CCANNNNNTGG	2 cut(s)	134, 1021
PkrI	GCNGC	5 cut(s)	173, 262, 305, 496, 825
PleI	GAGTC	2 cut(s)	12, 1199
PluTI	GGCGCC	1 cut(s)	385
PpsI	GAGTC	2 cut(s)	12, 1199
Ppu21I	YACGTR	1 cut(s)	558
PscI	ACATGT	2 cut(s)	330, 873
PshBI	ATTAAT	1 cut(s)	833
Psp124BI	GAGCTC	2 cut(s)	1014, 1419
Psp1406I	AACGTT	1 cut(s)	1284
Psp6I	CCWGG	2 cut(s)	109, 960
PspGI	CCWGG	2 cut(s)	109, 960
PspLI	CGTACG	1 cut(s)	336
PspN4I	GGNNCC	6 cut(s)	202, 383, 925, 959, 980, 1343
PspPI	GGNCC	7 cut(s)	113, 200, 371, 730, 957, 1118, 1209
PsuI	RGATCY	2 cut(s)	517, 1222
RsaI	GTAC	5 cut(s)	197, 329, 338, 560, 789
RsaNI	GTAC	5 cut(s)	196, 328, 337, 559, 788
SacI	GAGCTC	2 cut(s)	1014, 1419
SaqAI	TTAA	3 cut(s)	276, 833, 848
SatI	GCNGC	5 cut(s)	172, 261, 304, 495, 824
Sau3AI	GATC	5 cut(s)	517, 598, 757, 1033, 1222
Sau96I	GGNCC	7 cut(s)	113, 200, 371, 730, 957, 1118, 1209
SchI	GAGTC	2 cut(s)	13, 1200
ScrFI	CCNGG	3 cut(s)	111, 962, 983
SduI	GDGCHC	3 cut(s)	1014, 1346, 1419
SfaNI	GCATC	1 cut(s)	75
SfoI	GGCGCC	1 cut(s)	383
SfuI	TTCGAA	3 cut(s)	391, 741, 879
SinI	GGWCC	6 cut(s)	200, 371, 730, 957, 1118, 1209
SmlI	CTYRAG	4 cut(s)	85, 602, 973, 1075
SmoI	CTYRAG	4 cut(s)	85, 602, 973, 1075
Sse9I	AATT	3 cut(s)	714, 750, 1253
SsiI	CCGC	8 cut(s)	64, 169, 261, 304, 446, 495, 503, 1513
SspDI	GGCGCC	1 cut(s)	381
SspI	AATATT	1 cut(s)	1204
SspMI	CTAG	6 cut(s)	152, 185, 221, 300, 774, 1379
SstI	GAGCTC	2 cut(s)	1014, 1419
StyD4I	CCNGG	3 cut(s)	109, 960, 981
StyI	CCWWGG	3 cut(s)	220, 351, 1143
TaaI	ACNGT	2 cut(s)	727, 1372
TaiI	ACGT	5 cut(s)	560, 822, 1000, 1155, 1287
TaqI	TCGA	8 cut(s)	391, 432, 741, 879, 909, 1036, 1333, 1478
TasI	AATT	3 cut(s)	714, 750, 1253
TatI	WGTACW	2 cut(s)	327, 787
TauI	GCSGC	3 cut(s)	263, 306, 497
TfiI	GAWTC	2 cut(s)	429, 552
Tru1I	TTAA	3 cut(s)	276, 833, 848
Tru9I	TTAA	3 cut(s)	276, 833, 848
TseI	GCWGC	2 cut(s)	171, 823
TspDTI	ATGAA	7 cut(s)	134, 722, 777, 819, 1007, 1186, 1289
TspGWI	ACGGA	1 cut(s)	213
Van91I	CCANNNNNTGG	2 cut(s)	134, 1021
VpaK11BI	GGWCC	6 cut(s)	200, 371, 730, 957, 1118, 1209
VspI	ATTAAT	1 cut(s)	833
XagI	CCTNNNNNAGG	1 cut(s)	85
XapI	RAATTY	1 cut(s)	1253
XbaI	TCTAGA	1 cut(s)	773
XceI	RCATGY	3 cut(s)	334, 877, 1432
XmaJI	CCTAGG	1 cut(s)	220
XmiI	GTMKAC	2 cut(s)	816, 870
XmnI	GAANNNNTTC	1 cut(s)	1403
XspI	CTAG	6 cut(s)	152, 185, 221, 300, 774, 1379

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.