RchiOBHm_Chr6g0280251

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000282
Physical Location & Seq
Forward (+)
43562369 .. 43565415
3047 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ25132

Sequence Viewer

Length: 876 bp
ATGCCAACAATTGTCTACACCCTAGCCAACTCCGCTGAGCTCCAGGGATTGACCAAGCGCTTAAAGAAAATTAGCAAGACGATCGATCAACTCCTGGAGAAGATAATAGACGAGCATGAACAAGTTGCCAACACTAGGAGTGGGAAACAAGGCCATGAGTATTTTTTAGACGTGTTGCTTTCATTAACGAACCAACAGTTGAACCCGAATGATGAGCAAGTACACTTTCTTGATCAAACAAATGTGAAAGCCATCTTACTAGATATGATCACGGCGGCTTTTGATACTTCGGCCACATTGATTGTTTGGAGCCTTGCCGAGCTCTTGAGGCATCCAAGGATCATGAAGAATCTCCAAGAAGAGCTCCAAAGTGTGGTTGGTATGGACCGAATGGTGGAAGAATCTGATTTGCCCAAGCTGGATTACTTGAGTATGGTGGTGAAGGAGAGCTTCAGACTACACCCAGTTGCACCGTTACTAATCCCACATGAACCCATCGAGGACATTACAATCAATGGATATGACATTCCCAAGAAATCGCGGATCATAGTAAACATATGGAGCATTGGAAGAGATCCTAATGTTTGGTCCGAAAATGTTGAGGAATTTTATTCTGAAAGGCTCATGAACAGCAATATAGACCTCCGGGGACATGACTTTCAGCTCATCCCATTTGGGTCTGGGCGTAGAGGGTGTCCTGGTATGCAATTAGGACTAACCACAGTTCGGCTAGTTTTGGCACAGCTGGTGCACTGCTTTAACTGGGAGCTCCCAAATGGCTTGGTACCTCAAGACTTGGACATGTCTGAGGATTTCGTCTTATCACTGTCAAAAGCCAAACACTTGCTTGCTAAGCCAACGTACCGTCTTATGTAG

Protein Analysis

291

Amino Acids

33.21

Weight (kDa)

5.5

Isoelectric Point (pI)

39.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 16 - 281 3e-68 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000282)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g12350 FvH4_5g12380 FvH4_5g12392 FvH4_5g12400 FvH4_5g12430
malus_domestica MD06G1213300.v1.1 MD06G1213500.v1.1 MD06G1213700.v1.1 MD06G1213800.v1.1 MD06G1213900.v1.1 MD06G1214000.v1.1 MD06G1214400.v1.1 MD14G1224300.v1.1 MD14G1224400.v1.1 MD14G1224600.v1.1 MD14G1224700.v1.1 MD14G1224800.v1.1 MD14G1224900.v1.1 MD14G1225100.v1.1 MD14G1225400.v1.1
prunus_persica Prupe.5G219700_v2.0.a1 Prupe.5G219800_v2.0.a1 Prupe.5G219900_v2.0.a1 Prupe.8G078500_v2.0.a1
pyrus_communis pycom06g19100 pycom14g18590 pycom14g18600 pycom14g18610 pycom14g18660
rosa_chinensis RchiOBHm_Chr0c12g0499591 RchiOBHm_Chr2g0140591 RchiOBHm_Chr2g0140631 RchiOBHm_Chr2g0140641 RchiOBHm_Chr2g0140651 RchiOBHm_Chr4g0417991 RchiOBHm_Chr4g0418001 RchiOBHm_Chr4g0418101 RchiOBHm_Chr4g0418111 RchiOBHm_Chr4g0418121 RchiOBHm_Chr6g0280191 RchiOBHm_Chr6g0280241 RchiOBHm_Chr6g0280251 RchiOBHm_Chr7g0185031 RchiOBHm_Chr7g0185051 RchiOBHm_Chr7g0185081 RchiOBHm_Chr7g0185091 RchiOBHm_Chr7g0185111 RchiOBHm_Chr7g0185131 RchiOBHm_Chr7g0185151 RchiOBHm_Chr7g0185161 RchiOBHm_Chr7g0185191 RchiOBHm_Chr7g0222431
rosa_laevigata RLG00000002061 RLG00000004934 RLG00000004935 RLG00000004937 RLG00000013071 RLG00000013077 RLG00000019621 RLG00000019881 RLG00000019884 RLG00000019887 RLG00000019888 RLG00000028839
rosa_multiflora Rmu_co8014370.1_g000001 Rmu_co8334683.1_g000001 Rmu_co8440383.1_g000001 Rmu_sc0002826.1_g000008 Rmu_sc0002826.1_g000009 Rmu_sc0002826.1_g000030 Rmu_sc0004483.1_g000005 Rmu_sc0004638.1_g000003 Rmu_sc0004638.1_g000004 Rmu_sc0005756.1_g000003 Rmu_sc0006493.1_g000023 Rmu_sc0006493.1_g000024 Rmu_sc0007137.1_g000017 Rmu_sc0008496.1_g000022 Rmu_sc0008769.1_g000003 Rmu_sc0013612.1_g000026 Rmu_sc0013864.1_g000001 Rmu_sc0013864.1_g000007 Rmu_sc0013864.1_g000008 Rmu_sc0030128.1_g000001 Rmu_sc0041660.1_g000001 Rmu_ssc0000289.1_g000001
rosa_roxburghii Rroxscaffold_2G00104640 Rroxscaffold_3G00253970 Rroxscaffold_3G00269170 Rroxscaffold_3G00269200 Rroxscaffold_3G00269210 Rroxscaffold_3G00269220 Rroxscaffold_3G00269230 Rroxscaffold_7G00188320
rosa_rugosa Rorug01G0052300 Rorug05G0212000 Rorug06G0130700 Rorug06G0131100 Rorug06G0466200 Rorug06G0466300 Rorug06G0466400 Rorug06G0466500 Rorug06G0466700 Rorug07G0208500 Rorug07G0294600
rosa_samantha Rh4AG137600 Rh6DG237300 Rh6DG237800 Rh7AG076200 Rh7AG076300 Rh7AG076500 Rh7AG076600 Rh7AG350700 Rh7BG070800 Rh7BG070900 Rh7BG071000 Rh7BG071100
rosa_wichuraiana Rw6G020990 Rw6G021010 Rw7G005900 Rw7G005910 Rw7G005920 Rw7G005940 Rw7G005950 Rw7G005970 Rw7G029600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 784
AccB1I GGYRCC 1 cut(s) 784
AccB7I CCANNNNNTGG 1 cut(s) 373
AccI GTMKAC 1 cut(s) 15
AccII CGCG 1 cut(s) 541
AciI CCGC 3 cut(s) 33, 275, 541
AclWI GGATC 3 cut(s) 347, 551, 569
AcoI YGGCCR 1 cut(s) 291
AcsI RAATTY 1 cut(s) 605
AcuI CTGAAG 1 cut(s) 436
AfaI GTAC 3 cut(s) 222, 786, 863
AfeI AGCGCT 1 cut(s) 59
AfiI CCNNNNNNNGG 4 cut(s) 135, 373, 394, 726
AflIII ACRYGT 2 cut(s) 171, 801
AgsI TTSAA 1 cut(s) 202
AjiI CACGTC 1 cut(s) 172
AjnI CCWGG 3 cut(s) 42, 93, 697
AluBI AGCT 8 cut(s) 40, 322, 364, 418, 450, 664, 745, 769
AluI AGCT 8 cut(s) 40, 322, 364, 418, 450, 664, 745, 769
Alw21I GWGCWC 5 cut(s) 42, 324, 366, 753, 771
Alw44I GTGCAC 1 cut(s) 749
AlwI GGATC 3 cut(s) 347, 551, 569
Aor51HI AGCGCT 1 cut(s) 59
AoxI GGCC 2 cut(s) 151, 291
ApaLI GTGCAC 1 cut(s) 749
ApoI RAATTY 1 cut(s) 605
Asp718I GGTACC 1 cut(s) 784
AspLEI GCGC 1 cut(s) 60
AspS9I GGNCC 2 cut(s) 385, 588
AsuC2I CCSGG 1 cut(s) 647
AsuHPI GGTGA 1 cut(s) 451
AvaII GGWCC 2 cut(s) 385, 588
BaeGI GKGCMC 1 cut(s) 753
BanI GGYRCC 1 cut(s) 784
BanII GRGCYC 4 cut(s) 42, 324, 366, 771
Bbv12I GWGCWC 5 cut(s) 42, 324, 366, 753, 771
BccI CCATC 2 cut(s) 260, 503
BceAI ACGGC 1 cut(s) 288
BcgI CGANNNNNNTGC 2 cut(s) 64, 98
BciT130I CCWGG 3 cut(s) 44, 95, 699
BclI TGATCA 2 cut(s) 232, 267
BcnI CCSGG 1 cut(s) 647
BfaI CTAG 4 cut(s) 23, 135, 260, 731
BfoI RGCGCY 1 cut(s) 61
BisI GCNGC 1 cut(s) 276
BlpI GCTNAGC 2 cut(s) 36, 852
BlsI GCNGC 1 cut(s) 277
Bme1390I CCNGG 4 cut(s) 44, 95, 647, 699
Bme18I GGWCC 2 cut(s) 385, 588
BmgBI CACGTC 1 cut(s) 172
BmgT120I GGNCC 2 cut(s) 385, 588
BmiI GGNNCC 2 cut(s) 311, 786
BmrFI CCNGG 4 cut(s) 44, 95, 647, 699
BmrI ACTGGG 2 cut(s) 458, 772
BmsI GCATC 1 cut(s) 340
BmuI ACTGGG 2 cut(s) 458, 772
BpmI CTGGAG 2 cut(s) 26, 116
Bpu1102I GCTNAGC 2 cut(s) 36, 852
BpuEI CTTGAG 3 cut(s) 346, 448, 774
BpuMI CCSGG 1 cut(s) 647
Bsa29I ATCGAT 1 cut(s) 84
BsaJI CCNNGG 3 cut(s) 43, 335, 646
Bsc4I CCNNNNNNNGG 4 cut(s) 135, 373, 394, 726
Bse1I ACTGG 2 cut(s) 464, 767
BseBI CCWGG 3 cut(s) 44, 95, 699
BseCI ATCGAT 1 cut(s) 84
BseDI CCNNGG 3 cut(s) 43, 335, 646
BseGI GGATG 2 cut(s) 331, 666
BseLI CCNNNNNNNGG 4 cut(s) 135, 373, 394, 726
BseMII CTCAG 2 cut(s) 27, 798
BseNI ACTGG 2 cut(s) 464, 767
BseSI GKGCMC 1 cut(s) 753
Bsh1236I CGCG 1 cut(s) 541
Bsh1285I CGRYCG 1 cut(s) 84
BshFI GGCC 2 cut(s) 153, 293
BshNI GGYRCC 1 cut(s) 784
BshVI ATCGAT 1 cut(s) 84
BsiEI CGRYCG 1 cut(s) 84
BsiHKAI GWGCWC 5 cut(s) 42, 324, 366, 753, 771
BsiSI CCGG 1 cut(s) 646
BslFI GGGAC 1 cut(s) 663
BslI CCNNNNNNNGG 4 cut(s) 135, 373, 394, 726
BsmFI GGGAC 1 cut(s) 663
BsnI GGCC 2 cut(s) 153, 293
Bsp1286I GDGCHC 5 cut(s) 42, 324, 366, 753, 771
Bsp143I GATC 7 cut(s) 81, 85, 232, 267, 339, 543, 574
Bsp1720I GCTNAGC 2 cut(s) 36, 852
BspACI CCGC 3 cut(s) 33, 275, 541
BspANI GGCC 2 cut(s) 153, 293
BspCNI CTCAG 2 cut(s) 28, 799
BspDI ATCGAT 1 cut(s) 84
BspFNI CGCG 1 cut(s) 541
BspHI TCATGA 2 cut(s) 342, 624
BspLI GGNNCC 2 cut(s) 311, 786
BspPI GGATC 3 cut(s) 347, 551, 569
BspQI GCTCTTC 1 cut(s) 354
BspT107I GGYRCC 1 cut(s) 784
BsrI ACTGG 2 cut(s) 464, 767
BssECI CCNNGG 3 cut(s) 43, 335, 646
BssMI GATC 7 cut(s) 81, 85, 232, 267, 339, 543, 574
BssT1I CCWWGG 1 cut(s) 335
Bst2UI CCWGG 3 cut(s) 44, 95, 699
Bst4CI ACNGT 5 cut(s) 198, 474, 724, 828, 866
Bst6I CTCTTC 2 cut(s) 354, 565
BstC8I GCNNGC 1 cut(s) 849
BstDEI CTNAG 3 cut(s) 36, 807, 852
BstF5I GGATG 2 cut(s) 331, 666
BstFNI CGCG 1 cut(s) 541
BstH2I RGCGCY 1 cut(s) 61
BstHHI GCGC 1 cut(s) 60
BstKTI GATC 7 cut(s) 84, 88, 235, 270, 342, 546, 577
BstMBI GATC 7 cut(s) 81, 85, 232, 267, 339, 543, 574
BstMCI CGRYCG 1 cut(s) 84
BstMWI GCNNNNNNNGC 3 cut(s) 32, 328, 853
BstNI CCWGG 3 cut(s) 44, 95, 699
BstNSI RCATGY 1 cut(s) 805
BstSCI CCNGG 4 cut(s) 42, 93, 645, 697
BstSLI GKGCMC 1 cut(s) 753
BstUI CGCG 1 cut(s) 541
BstX2I RGATCY 1 cut(s) 574
BstYI RGATCY 1 cut(s) 574
Bsu15I ATCGAT 1 cut(s) 84
BsuRI GGCC 2 cut(s) 153, 293
BsuTUI ATCGAT 1 cut(s) 84
BtrI CACGTC 1 cut(s) 172
BtsCI GGATG 2 cut(s) 331, 666
BtsI GCAGTG 1 cut(s) 751
BtsIMutI CAGTG 2 cut(s) 751, 824
Cac8I GCNNGC 1 cut(s) 849
CciI TCATGA 2 cut(s) 342, 624
CfoI GCGC 1 cut(s) 60
Cfr13I GGNCC 2 cut(s) 385, 588
ClaI ATCGAT 1 cut(s) 84
Csp6I GTAC 3 cut(s) 221, 785, 862
CspCI CAANNNNNGTGG 2 cut(s) 283, 318
CviAII CATG 7 cut(s) 116, 155, 343, 488, 625, 653, 802
CviQI GTAC 3 cut(s) 221, 785, 862
DdeI CTNAG 3 cut(s) 36, 807, 852
DpnI GATC 7 cut(s) 83, 87, 234, 269, 341, 545, 576
DpnII GATC 7 cut(s) 81, 85, 232, 267, 339, 543, 574
EaeI YGGCCR 1 cut(s) 291
Eam1104I CTCTTC 2 cut(s) 354, 565
EarI CTCTTC 2 cut(s) 354, 565
Ecl136II GAGCTC 4 cut(s) 40, 322, 364, 769
Eco130I CCWWGG 1 cut(s) 335
Eco24I GRGCYC 4 cut(s) 42, 324, 366, 771
Eco47I GGWCC 2 cut(s) 385, 588
Eco47III AGCGCT 1 cut(s) 59
Eco53kI GAGCTC 4 cut(s) 40, 322, 364, 769
Eco57I CTGAAG 1 cut(s) 436
EcoICRI GAGCTC 4 cut(s) 40, 322, 364, 769
EcoRII CCWGG 3 cut(s) 42, 93, 697
EcoT14I CCWWGG 1 cut(s) 335
EcoT38I GRGCYC 4 cut(s) 42, 324, 366, 771
ErhI CCWWGG 1 cut(s) 335
FaeI CATG 7 cut(s) 119, 158, 346, 491, 628, 656, 805
FalI AAGNNNNNCTT 2 cut(s) 434, 466
FaqI GGGAC 1 cut(s) 663
FatI CATG 7 cut(s) 115, 154, 342, 487, 624, 652, 801
FauNDI CATATG 1 cut(s) 557
FbaI TGATCA 2 cut(s) 232, 267
FblI GTMKAC 1 cut(s) 15
Fnu4HI GCNGC 1 cut(s) 276
FokI GGATG 2 cut(s) 318, 653
FriOI GRGCYC 4 cut(s) 42, 324, 366, 771
Fsp4HI GCNGC 1 cut(s) 276
FspBI CTAG 4 cut(s) 23, 135, 260, 731
GlaI GCGC 1 cut(s) 59
GluI GCNGC 1 cut(s) 276
GsuI CTGGAG 2 cut(s) 26, 116
HaeII RGCGCY 1 cut(s) 61
HaeIII GGCC 2 cut(s) 153, 293
HapII CCGG 1 cut(s) 646
HhaI GCGC 1 cut(s) 60
Hin1II CATG 7 cut(s) 119, 158, 346, 491, 628, 656, 805
Hin6I GCGC 1 cut(s) 58
HinP1I GCGC 1 cut(s) 58
HinfI GANTC 2 cut(s) 349, 401
HpaII CCGG 1 cut(s) 646
HphI GGTGA 1 cut(s) 451
Hpy166II GTNNAC 4 cut(s) 16, 223, 553, 751
Hpy188I TCNGA 5 cut(s) 406, 455, 592, 616, 808
Hpy188III TCNNGA 5 cut(s) 230, 325, 343, 625, 791
Hpy8I GTNNAC 4 cut(s) 16, 223, 553, 751
HpyAV CCTTC 1 cut(s) 436
HpyCH4III ACNGT 5 cut(s) 198, 474, 724, 828, 866
HpyCH4IV ACGT 2 cut(s) 171, 860
HpyCH4V TGCA 3 cut(s) 470, 706, 751
HpyF10VI GCNNNNNNNGC 3 cut(s) 32, 328, 853
HpyF3I CTNAG 3 cut(s) 36, 807, 852
HpySE526I ACGT 2 cut(s) 171, 860
Hsp92II CATG 7 cut(s) 119, 158, 346, 491, 628, 656, 805
HspAI GCGC 1 cut(s) 58
KpnI GGTACC 1 cut(s) 788
Ksp22I TGATCA 2 cut(s) 232, 267
Kzo9I GATC 7 cut(s) 81, 85, 232, 267, 339, 543, 574
LguI GCTCTTC 1 cut(s) 354
LmnI GCTCC 6 cut(s) 45, 309, 369, 561, 766, 774
LweI GCATC 1 cut(s) 340
MaeI CTAG 4 cut(s) 23, 135, 260, 731
MaeII ACGT 2 cut(s) 171, 860
MaeIII GTNAC 1 cut(s) 474
MalI GATC 7 cut(s) 83, 87, 234, 269, 341, 545, 576
MboI GATC 7 cut(s) 81, 85, 232, 267, 339, 543, 574
MboII GAAGA 5 cut(s) 112, 358, 371, 410, 582
MfeI CAATTG 1 cut(s) 9
MflI RGATCY 1 cut(s) 574
MhlI GDGCHC 5 cut(s) 42, 324, 366, 753, 771
MluCI AATT 4 cut(s) 9, 69, 605, 707
MnlI CCTC 7 cut(s) 321, 493, 595, 653, 683, 798, 802
MseI TTAA 3 cut(s) 62, 185, 759
MspA1I CMGCKG 2 cut(s) 35, 745
MspI CCGG 1 cut(s) 646
MspR9I CCNGG 4 cut(s) 44, 95, 647, 699
MunI CAATTG 1 cut(s) 9
MvaI CCWGG 3 cut(s) 44, 95, 699
MvnI CGCG 1 cut(s) 541
MwoI GCNNNNNNNGC 3 cut(s) 32, 328, 853
NciI CCSGG 1 cut(s) 647
NdeI CATATG 1 cut(s) 557
NdeII GATC 7 cut(s) 81, 85, 232, 267, 339, 543, 574
NlaIII CATG 7 cut(s) 119, 158, 346, 491, 628, 656, 805
NlaIV GGNNCC 2 cut(s) 311, 786
NmeAIII GCCGAG 1 cut(s) 343
NspI RCATGY 1 cut(s) 805
PagI TCATGA 2 cut(s) 342, 624
PciI ACATGT 1 cut(s) 801
PciSI GCTCTTC 1 cut(s) 354
PfeI GAWTC 2 cut(s) 349, 401
PflMI CCANNNNNTGG 1 cut(s) 373
PfoI TCCNGGA 1 cut(s) 93
PkrI GCNGC 1 cut(s) 277
Ple19I CGATCG 1 cut(s) 84
PscI ACATGT 1 cut(s) 801
Psp124BI GAGCTC 4 cut(s) 42, 324, 366, 771
Psp6I CCWGG 3 cut(s) 42, 93, 697
PspGI CCWGG 3 cut(s) 42, 93, 697
PspN4I GGNNCC 2 cut(s) 311, 786
PspPI GGNCC 2 cut(s) 385, 588
PsuI RGATCY 1 cut(s) 574
PvuI CGATCG 1 cut(s) 84
PvuII CAGCTG 1 cut(s) 745
RsaI GTAC 3 cut(s) 222, 786, 863
RsaNI GTAC 3 cut(s) 221, 785, 862
SacI GAGCTC 4 cut(s) 42, 324, 366, 771
SapI GCTCTTC 1 cut(s) 354
SaqAI TTAA 3 cut(s) 62, 185, 759
SatI GCNGC 1 cut(s) 276
Sau3AI GATC 7 cut(s) 81, 85, 232, 267, 339, 543, 574
Sau96I GGNCC 2 cut(s) 385, 588
ScrFI CCNGG 4 cut(s) 44, 95, 647, 699
SduI GDGCHC 5 cut(s) 42, 324, 366, 753, 771
SfaNI GCATC 1 cut(s) 340
SinI GGWCC 2 cut(s) 385, 588
SmlI CTYRAG 3 cut(s) 325, 427, 789
SmoI CTYRAG 3 cut(s) 325, 427, 789
Sse9I AATT 4 cut(s) 9, 69, 605, 707
SsiI CCGC 3 cut(s) 33, 275, 541
SspMI CTAG 4 cut(s) 23, 135, 260, 731
SstI GAGCTC 4 cut(s) 42, 324, 366, 771
StyD4I CCNGG 4 cut(s) 42, 93, 645, 697
StyI CCWWGG 1 cut(s) 335
TaaI ACNGT 5 cut(s) 198, 474, 724, 828, 866
TaiI ACGT 2 cut(s) 174, 863
TaqI TCGA 2 cut(s) 84, 498
TaqII GACCGA 1 cut(s) 402
TasI AATT 4 cut(s) 9, 69, 605, 707
TatI WGTACW 1 cut(s) 220
TauI GCSGC 1 cut(s) 278
TfiI GAWTC 2 cut(s) 349, 401
Tru1I TTAA 3 cut(s) 62, 185, 759
Tru9I TTAA 3 cut(s) 62, 185, 759
TscAI CASTG 2 cut(s) 758, 831
TspDTI ATGAA 5 cut(s) 132, 171, 359, 504, 641
TspRI CASTG 2 cut(s) 758, 831
Van91I CCANNNNNTGG 1 cut(s) 373
VneI GTGCAC 1 cut(s) 749
VpaK11BI GGWCC 2 cut(s) 385, 588
XapI RAATTY 1 cut(s) 605
XceI RCATGY 1 cut(s) 805
XcmI CCANNNNNNNNNTGG 1 cut(s) 374
XmiI GTMKAC 1 cut(s) 15
XspI CTAG 4 cut(s) 23, 135, 260, 731
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.