RchiOBHm_Chr7g0185031

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0000282
Physical Location & Seq
Forward (+)
5309837 .. 5314679
4843 bp
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UTR
Exon/CDS
Intron
PRQ16510

Sequence Viewer

Length: 1644 bp
ATGCCTCAGAAAAGCACGCAGAATCTAACTGCAGATACATGCACTGATACACGTTTATTAATCTATAAATATCTATCTAGTTTCACATGTTTTCCAAAAGAAAAAATCTATCTAGTTTCAACAGACACAATGAGTCCTTCAACAATTACCATCCTTCTCCTCTTCGTTGCCCTCATCTGGTTTCTCATCGCTGCAGCCTTCTCAAAACCAAAGCACAAGAAGCTACCTCCTGGACCTTTGGCACTACCGGTCATTGGTCACCTTCATATGCTAGGCAATCTCCCACATCGCAGCCTCCAACACTTGGCCAAAAAATATGGACCCATAATGTCTATTCGCCTTGGGAATGTACCCACCATACTAGTCTCGTCCCCGAAAGCTGCAGAGCTATTCCTCAAAACTCATGACGCCAATTTCGCGAGCAGGCCGACAGTCCAGGCCTCAGAGCACCTAGCTTACGGCACAATGGGCCTGGCGTTTGCCAAGTACGGCCCCTATTGGCGCCATGTGAGGAAGCTCTGTACCCTCGAGCTTCTTTGTCCCTCGAAAGCGGAGGCTTTTGCGCCGCTGCGAAGGGAGGAGATAGAACTGTTGGTGCAGTCGCTGAAGAAAGCTGGAGAGGGAGGTGAGGTGGTGGATGTTAGTGACAAGATTTATGGCGTTGGTGAGGATATAACGTATAGGATGATATTGGGTTGCACAAGGGATGATGCTAGGTTTGGTTTGAAGGAGATTGTTCACGAAGTTTCGTTGTTAACCGGGGCATTTAACGTAGCTGATTTTGTGCCCTGCCTCGGTCCATTTGATTTTCAGGGATTGACCAAGCGCTTAAAGAAAGTTAGCAAGACGATCAATCAACTCTTGGAGAAGATAATAGATGAGCATGAACAAGTTGCCAACACCAAGAGTGGGAAACAAGGCCATGAGGACTTTGTAGACGTGTTGCTTGCGTTAACGAACCGACCGTTGAACCAGAATGATGAGCAAGTACACTTCTTTGATCGAACAAATGTGAAAGCCATCTTACTAGACCTGATAACGGCGGCTTTTGATACTTCGGCCACATCGATTGTTTGGAGCCTTGCCGAGCTCTTGAGGCATCCAAGGATCATGAAGAATCTCCAAGAAGAGATCCAAAGTGTGGTTGGTATGGACCGAATGGTGGAAGAATCTGATTTGCCCAAGCTGGATTACTTGAGTATGGTGGTAAAGGAGAGCTTCAGACTACACCCAGTTGCACCGTTACTAGTCCCACATGAATCCATCGAGGACATTACCATCGATGGATATGACATACCCAAGAAGTCGCGGATCATAGTAAACATCTGGAGCATTGGAAGAGATCCTAATGTTTGGTCAGAAAATGTTGAGGAGTTTTATCCTGAAAGGTTCATGAACAACAATATAGACCTCCGGGGACATAACTTTCAGCTCATCCCATTTGGGTCTGGGCGAAGAGGGTGTCCGGGTATGCAATTAGGGCTAACCACAGTTCGACTAGTTCTGGCACAACTGGTTCATTGCTTTAACTGGGAGCTCCCAAATGGCTTGCTACCTCAAGACTTGGACATGTCCGAGGATTTTGGCTTATCACTGTCAAAAGCCAAACACTTGCTTGCTAAGCCAACGTACCGTCTTACGTAG

Protein Analysis

547

Amino Acids

61.52

Weight (kDa)

7.29

Isoelectric Point (pI)

39.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 76 - 534 2.1e-103 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000282)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g12350 FvH4_5g12380 FvH4_5g12392 FvH4_5g12400 FvH4_5g12430
malus_domestica MD06G1213300.v1.1 MD06G1213500.v1.1 MD06G1213700.v1.1 MD06G1213800.v1.1 MD06G1213900.v1.1 MD06G1214000.v1.1 MD06G1214400.v1.1 MD14G1224300.v1.1 MD14G1224400.v1.1 MD14G1224600.v1.1 MD14G1224700.v1.1 MD14G1224800.v1.1 MD14G1224900.v1.1 MD14G1225100.v1.1 MD14G1225400.v1.1
prunus_persica Prupe.5G219700_v2.0.a1 Prupe.5G219800_v2.0.a1 Prupe.5G219900_v2.0.a1 Prupe.8G078500_v2.0.a1
pyrus_communis pycom06g19100 pycom14g18590 pycom14g18600 pycom14g18610 pycom14g18660
rosa_chinensis RchiOBHm_Chr0c12g0499591 RchiOBHm_Chr2g0140591 RchiOBHm_Chr2g0140631 RchiOBHm_Chr2g0140641 RchiOBHm_Chr2g0140651 RchiOBHm_Chr4g0417991 RchiOBHm_Chr4g0418001 RchiOBHm_Chr4g0418101 RchiOBHm_Chr4g0418111 RchiOBHm_Chr4g0418121 RchiOBHm_Chr6g0280191 RchiOBHm_Chr6g0280241 RchiOBHm_Chr6g0280251 RchiOBHm_Chr7g0185031 RchiOBHm_Chr7g0185051 RchiOBHm_Chr7g0185081 RchiOBHm_Chr7g0185091 RchiOBHm_Chr7g0185111 RchiOBHm_Chr7g0185131 RchiOBHm_Chr7g0185151 RchiOBHm_Chr7g0185161 RchiOBHm_Chr7g0185191 RchiOBHm_Chr7g0222431
rosa_laevigata RLG00000002061 RLG00000004934 RLG00000004935 RLG00000004937 RLG00000013071 RLG00000013077 RLG00000019621 RLG00000019881 RLG00000019884 RLG00000019887 RLG00000019888 RLG00000028839
rosa_multiflora Rmu_co8014370.1_g000001 Rmu_co8334683.1_g000001 Rmu_co8440383.1_g000001 Rmu_sc0002826.1_g000008 Rmu_sc0002826.1_g000009 Rmu_sc0002826.1_g000030 Rmu_sc0004483.1_g000005 Rmu_sc0004638.1_g000003 Rmu_sc0004638.1_g000004 Rmu_sc0005756.1_g000003 Rmu_sc0006493.1_g000023 Rmu_sc0006493.1_g000024 Rmu_sc0007137.1_g000017 Rmu_sc0008496.1_g000022 Rmu_sc0008769.1_g000003 Rmu_sc0013612.1_g000026 Rmu_sc0013864.1_g000001 Rmu_sc0013864.1_g000007 Rmu_sc0013864.1_g000008 Rmu_sc0030128.1_g000001 Rmu_sc0041660.1_g000001 Rmu_ssc0000289.1_g000001
rosa_roxburghii Rroxscaffold_2G00104640 Rroxscaffold_3G00253970 Rroxscaffold_3G00269170 Rroxscaffold_3G00269200 Rroxscaffold_3G00269210 Rroxscaffold_3G00269220 Rroxscaffold_3G00269230 Rroxscaffold_7G00188320
rosa_rugosa Rorug01G0052300 Rorug05G0212000 Rorug06G0130700 Rorug06G0131100 Rorug06G0466200 Rorug06G0466300 Rorug06G0466400 Rorug06G0466500 Rorug06G0466700 Rorug07G0208500 Rorug07G0294600
rosa_samantha Rh4AG137600 Rh6DG237300 Rh6DG237800 Rh7AG076200 Rh7AG076300 Rh7AG076500 Rh7AG076600 Rh7AG350700 Rh7BG070800 Rh7BG070900 Rh7BG071000 Rh7BG071100
rosa_wichuraiana Rw6G020990 Rw6G021010 Rw7G005900 Rw7G005910 Rw7G005920 Rw7G005940 Rw7G005950 Rw7G005970 Rw7G029600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 501
AccB7I CCANNNNNTGG 2 cut(s) 304, 1141
AccI GTMKAC 1 cut(s) 936
AccII CGCG 2 cut(s) 419, 1309
AciI CCGC 4 cut(s) 551, 566, 1043, 1309
AclWI GGATC 4 cut(s) 1115, 1126, 1319, 1337
AcoI YGGCCR 2 cut(s) 306, 1059
AcuI CTGAAG 2 cut(s) 626, 1204
AcyI GRCGYC 2 cut(s) 408, 502
AfaI GTAC 5 cut(s) 351, 488, 523, 990, 1631
AfeI AGCGCT 1 cut(s) 827
AfiI CCNNNNNNNGG 8 cut(s) 177, 254, 304, 794, 909, 1039, 1141, 1162
AflIII ACRYGT 4 cut(s) 50, 86, 939, 1569
AgeI ACCGGT 1 cut(s) 247
AgsI TTSAA 4 cut(s) 120, 141, 727, 970
AhlI ACTAGT 3 cut(s) 361, 1246, 1498
AjiI CACGTC 1 cut(s) 940
AjnI CCWGG 3 cut(s) 229, 435, 471
Alw21I GWGCWC 3 cut(s) 450, 1092, 1539
Alw26I GTCTC 1 cut(s) 370
AlwI GGATC 4 cut(s) 1115, 1126, 1319, 1337
AlwNI CAGNNNCTG 1 cut(s) 604
Ama87I CYCGRG 1 cut(s) 527
Aor51HI AGCGCT 1 cut(s) 827
AoxI GGCC 7 cut(s) 306, 425, 438, 469, 490, 919, 1059
ApeKI GCWGC 5 cut(s) 191, 194, 291, 380, 568
ArsI GACNNNNNNTTYG 4 cut(s) 398, 430, 1447, 1479
AseI ATTAAT 1 cut(s) 59
AsiGI ACCGGT 1 cut(s) 247
AspLEI GCGC 3 cut(s) 504, 565, 828
AspS9I GGNCC 6 cut(s) 233, 320, 469, 491, 797, 1153
AsuC2I CCSGG 3 cut(s) 760, 1415, 1467
AsuHPI GGTGA 3 cut(s) 251, 638, 677
AvaI CYCGRG 1 cut(s) 527
AvaII GGWCC 4 cut(s) 233, 320, 797, 1153
BaeGI GKGCMC 1 cut(s) 789
BalI TGGCCA 1 cut(s) 308
BanI GGYRCC 1 cut(s) 501
BanII GRGCYC 2 cut(s) 1092, 1539
Bbv12I GWGCWC 3 cut(s) 450, 1092, 1539
BbvI GCAGC 5 cut(s) 178, 206, 303, 367, 555
BccI CCATC 5 cut(s) 158, 1028, 1271, 1277, 1286
BceAI ACGGC 3 cut(s) 475, 505, 1056
BciT130I CCWGG 3 cut(s) 231, 437, 473
BcnI CCSGG 3 cut(s) 760, 1415, 1467
BcoDI GTCTC 1 cut(s) 370
BcuI ACTAGT 3 cut(s) 361, 1246, 1498
BfaI CTAG 9 cut(s) 78, 113, 272, 362, 452, 714, 1028, 1247, 1499
BfmI CTRYAG 3 cut(s) 30, 192, 381
BfoI RGCGCY 2 cut(s) 505, 829
BisI GCNGC 7 cut(s) 192, 195, 292, 381, 566, 569, 1044
BlpI GCTNAGC 1 cut(s) 1620
BlsI GCNGC 7 cut(s) 193, 196, 293, 382, 567, 570, 1045
Bme1390I CCNGG 6 cut(s) 231, 437, 473, 760, 1415, 1467
Bme18I GGWCC 4 cut(s) 233, 320, 797, 1153
BmeT110I CYCGRG 1 cut(s) 527
BmgBI CACGTC 1 cut(s) 940
BmgT120I GGNCC 6 cut(s) 233, 320, 469, 491, 797, 1153
BmiI GGNNCC 4 cut(s) 322, 493, 503, 1079
BmrFI CCNGG 6 cut(s) 231, 437, 473, 760, 1415, 1467
BmrI ACTGGG 2 cut(s) 1226, 1540
BmsI GCATC 2 cut(s) 700, 1108
BmuI ACTGGG 2 cut(s) 1226, 1540
BpmI CTGGAG 2 cut(s) 636, 1348
Bpu1102I GCTNAGC 1 cut(s) 1620
BpuEI CTTGAG 3 cut(s) 1114, 1216, 1542
BpuMI CCSGG 3 cut(s) 760, 1415, 1467
Bsa29I ATCGAT 2 cut(s) 1067, 1281
BsaAI YACGTR 1 cut(s) 1641
BsaHI GRCGYC 2 cut(s) 408, 502
BsaJI CCNNGG 6 cut(s) 340, 759, 793, 1103, 1414, 1575
BsaWI WCCGGW 1 cut(s) 247
BsaXI ACNNNNNCTCC 2 cut(s) 615, 645
Bsc4I CCNNNNNNNGG 8 cut(s) 177, 254, 304, 794, 909, 1039, 1141, 1162
Bse118I RCCGGY 1 cut(s) 247
Bse1I ACTGG 3 cut(s) 1232, 1518, 1535
Bse3DI GCAATG 1 cut(s) 1519
BseBI CCWGG 3 cut(s) 231, 437, 473
BseCI ATCGAT 2 cut(s) 1067, 1281
BseDI CCNNGG 6 cut(s) 340, 759, 793, 1103, 1414, 1575
BseGI GGATG 6 cut(s) 150, 643, 690, 712, 1099, 1434
BseLI CCNNNNNNNGG 8 cut(s) 177, 254, 304, 794, 909, 1039, 1141, 1162
BseMI GCAATG 1 cut(s) 1519
BseMII CTCAG 2 cut(s) 20, 456
BseNI ACTGG 3 cut(s) 1232, 1518, 1535
BseRI GAGGAG 3 cut(s) 149, 593, 1385
BseSI GKGCMC 1 cut(s) 789
BseXI GCAGC 5 cut(s) 178, 206, 303, 367, 555
BsgI GTGCAG 1 cut(s) 617
Bsh1236I CGCG 2 cut(s) 419, 1309
Bsh1285I CGRYCG 1 cut(s) 965
BshFI GGCC 7 cut(s) 308, 427, 440, 471, 492, 921, 1061
BshNI GGYRCC 1 cut(s) 501
BshTI ACCGGT 1 cut(s) 247
BshVI ATCGAT 2 cut(s) 1067, 1281
BsiEI CGRYCG 1 cut(s) 965
BsiHKAI GWGCWC 3 cut(s) 450, 1092, 1539
BsiHKCI CYCGRG 1 cut(s) 527
BsiSI CCGG 4 cut(s) 248, 759, 1414, 1466
BslFI GGGAC 4 cut(s) 355, 525, 1235, 1431
BslI CCNNNNNNNGG 8 cut(s) 177, 254, 304, 794, 909, 1039, 1141, 1162
BsmAI GTCTC 1 cut(s) 370
BsmFI GGGAC 4 cut(s) 355, 525, 1235, 1431
BsnI GGCC 7 cut(s) 308, 427, 440, 471, 492, 921, 1061
BsoBI CYCGRG 1 cut(s) 527
Bsp1286I GDGCHC 4 cut(s) 450, 789, 1092, 1539
Bsp143I GATC 6 cut(s) 849, 1000, 1107, 1131, 1311, 1342
Bsp1720I GCTNAGC 1 cut(s) 1620
Bsp68I TCGCGA 1 cut(s) 419
BspACI CCGC 4 cut(s) 551, 566, 1043, 1309
BspANI GGCC 7 cut(s) 308, 427, 440, 471, 492, 921, 1061
BspCNI CTCAG 2 cut(s) 19, 455
BspDI ATCGAT 2 cut(s) 1067, 1281
BspFNI CGCG 2 cut(s) 419, 1309
BspHI TCATGA 3 cut(s) 403, 1110, 1392
BspLI GGNNCC 4 cut(s) 322, 493, 503, 1079
BspMAI CTGCAG 3 cut(s) 34, 196, 385
BspPI GGATC 4 cut(s) 1115, 1126, 1319, 1337
BspT107I GGYRCC 1 cut(s) 501
BsrDI GCAATG 1 cut(s) 1519
BsrFI RCCGGY 1 cut(s) 247
BsrI ACTGG 3 cut(s) 1232, 1518, 1535
BssAI RCCGGY 1 cut(s) 247
BssECI CCNNGG 6 cut(s) 340, 759, 793, 1103, 1414, 1575
BssMI GATC 6 cut(s) 849, 1000, 1107, 1131, 1311, 1342
BssNI GRCGYC 2 cut(s) 408, 502
BssT1I CCWWGG 2 cut(s) 340, 1103
Bst2UI CCWGG 3 cut(s) 231, 437, 473
Bst4CI ACNGT 7 cut(s) 433, 591, 966, 1242, 1492, 1596, 1634
Bst6I CTCTTC 4 cut(s) 167, 1122, 1333, 1450
BstACI GRCGYC 2 cut(s) 408, 502
BstBAI YACGTR 1 cut(s) 1641
BstC8I GCNNGC 6 cut(s) 17, 421, 425, 948, 1550, 1617
BstDEI CTNAG 3 cut(s) 6, 442, 1620
BstEII GGTNACC 1 cut(s) 257
BstF5I GGATG 6 cut(s) 150, 643, 690, 712, 1099, 1434
BstFNI CGCG 2 cut(s) 419, 1309
BstH2I RGCGCY 2 cut(s) 505, 829
BstHHI GCGC 3 cut(s) 504, 565, 828
BstKTI GATC 6 cut(s) 852, 1003, 1110, 1134, 1314, 1345
BstMAI GTCTC 1 cut(s) 370
BstMBI GATC 6 cut(s) 849, 1000, 1107, 1131, 1311, 1342
BstMCI CGRYCG 1 cut(s) 965
BstMWI GCNNNNNNNGC 6 cut(s) 220, 416, 468, 1096, 1480, 1621
BstNI CCWGG 3 cut(s) 231, 437, 473
BstNSI RCATGY 3 cut(s) 42, 90, 1573
BstPI GGTNACC 1 cut(s) 257
BstSCI CCNGG 6 cut(s) 229, 435, 471, 758, 1413, 1465
BstSFI CTRYAG 3 cut(s) 30, 192, 381
BstSLI GKGCMC 1 cut(s) 789
BstSNI TACGTA 1 cut(s) 1641
BstUI CGCG 2 cut(s) 419, 1309
BstV1I GCAGC 5 cut(s) 178, 206, 303, 367, 555
BstX2I RGATCY 2 cut(s) 1131, 1342
BstYI RGATCY 2 cut(s) 1131, 1342
Bsu15I ATCGAT 2 cut(s) 1067, 1281
BsuRI GGCC 7 cut(s) 308, 427, 440, 471, 492, 921, 1061
BsuTUI ATCGAT 2 cut(s) 1067, 1281
BtgZI GCGATG 2 cut(s) 172, 272
BtrI CACGTC 1 cut(s) 940
BtsCI GGATG 6 cut(s) 150, 643, 690, 712, 1099, 1434
BtsIMutI CAGTG 2 cut(s) 42, 1592
BtuMI TCGCGA 1 cut(s) 419
Cac8I GCNNGC 6 cut(s) 17, 421, 425, 948, 1550, 1617
CaiI CAGNNNCTG 1 cut(s) 604
CciI TCATGA 3 cut(s) 403, 1110, 1392
CfoI GCGC 3 cut(s) 504, 565, 828
Cfr10I RCCGGY 1 cut(s) 247
Cfr13I GGNCC 6 cut(s) 233, 320, 469, 491, 797, 1153
ClaI ATCGAT 2 cut(s) 1067, 1281
CseI GACGC 1 cut(s) 416
Csp6I GTAC 5 cut(s) 350, 487, 522, 989, 1630
CspAI ACCGGT 1 cut(s) 247
CspCI CAANNNNNGTGG 2 cut(s) 1051, 1086
CviQI GTAC 5 cut(s) 350, 487, 522, 989, 1630
DdeI CTNAG 3 cut(s) 6, 442, 1620
DinI GGCGCC 1 cut(s) 503
DpnI GATC 6 cut(s) 851, 1002, 1109, 1133, 1313, 1344
DpnII GATC 6 cut(s) 849, 1000, 1107, 1131, 1311, 1342
EaeI YGGCCR 2 cut(s) 306, 1059
Eam1104I CTCTTC 4 cut(s) 167, 1122, 1333, 1450
EarI CTCTTC 4 cut(s) 167, 1122, 1333, 1450
Ecl136II GAGCTC 2 cut(s) 1090, 1537
Eco105I TACGTA 1 cut(s) 1641
Eco130I CCWWGG 2 cut(s) 340, 1103
Eco147I AGGCCT 1 cut(s) 440
Eco24I GRGCYC 2 cut(s) 1092, 1539
Eco47I GGWCC 4 cut(s) 233, 320, 797, 1153
Eco47III AGCGCT 1 cut(s) 827
Eco53kI GAGCTC 2 cut(s) 1090, 1537
Eco57I CTGAAG 2 cut(s) 626, 1204
Eco88I CYCGRG 1 cut(s) 527
Eco91I GGTNACC 1 cut(s) 257
EcoICRI GAGCTC 2 cut(s) 1090, 1537
EcoO65I GGTNACC 1 cut(s) 257
EcoRII CCWGG 3 cut(s) 229, 435, 471
EcoT14I CCWWGG 2 cut(s) 340, 1103
EcoT38I GRGCYC 2 cut(s) 1092, 1539
EgeI GGCGCC 1 cut(s) 503
EheI GGCGCC 1 cut(s) 503
ErhI CCWWGG 2 cut(s) 340, 1103
FalI AAGNNNNNCTT 2 cut(s) 1202, 1234
FaqI GGGAC 4 cut(s) 355, 525, 1235, 1431
FauNDI CATATG 1 cut(s) 267
FblI GTMKAC 1 cut(s) 936
Fnu4HI GCNGC 7 cut(s) 192, 195, 292, 381, 566, 569, 1044
FokI GGATG 6 cut(s) 137, 650, 697, 719, 1086, 1421
FriOI GRGCYC 2 cut(s) 1092, 1539
Fsp4HI GCNGC 7 cut(s) 192, 195, 292, 381, 566, 569, 1044
FspBI CTAG 9 cut(s) 78, 113, 272, 362, 452, 714, 1028, 1247, 1499
GlaI GCGC 3 cut(s) 503, 564, 827
GluI GCNGC 7 cut(s) 192, 195, 292, 381, 566, 569, 1044
GsuI CTGGAG 2 cut(s) 636, 1348
HaeII RGCGCY 2 cut(s) 505, 829
HaeIII GGCC 7 cut(s) 308, 427, 440, 471, 492, 921, 1061
HapII CCGG 4 cut(s) 248, 759, 1414, 1466
HgaI GACGC 1 cut(s) 416
HhaI GCGC 3 cut(s) 504, 565, 828
Hin1I GRCGYC 2 cut(s) 408, 502
Hin6I GCGC 3 cut(s) 502, 563, 826
HinP1I GCGC 3 cut(s) 502, 563, 826
HincII GTYRAC 2 cut(s) 756, 954
HindII GTYRAC 2 cut(s) 756, 954
HinfI GANTC 5 cut(s) 22, 133, 1117, 1169, 1259
HpaI GTTAAC 2 cut(s) 756, 954
HpaII CCGG 4 cut(s) 248, 759, 1414, 1466
HphI GGTGA 3 cut(s) 251, 638, 677
Hpy166II GTNNAC 6 cut(s) 739, 756, 937, 954, 991, 1321
Hpy188I TCNGA 6 cut(s) 9, 445, 1174, 1223, 1360, 1576
Hpy188III TCNNGA 9 cut(s) 404, 418, 740, 1093, 1111, 1327, 1382, 1393, 1559
Hpy8I GTNNAC 6 cut(s) 739, 756, 937, 954, 991, 1321
HpyAV CCTTC 6 cut(s) 147, 164, 208, 272, 567, 721
HpyCH4III ACNGT 7 cut(s) 433, 591, 966, 1242, 1492, 1596, 1634
HpyCH4IV ACGT 6 cut(s) 52, 677, 771, 939, 1628, 1640
HpyCH4V TGCA 8 cut(s) 32, 42, 194, 383, 598, 699, 1238, 1474
HpyF10VI GCNNNNNNNGC 6 cut(s) 220, 416, 468, 1096, 1480, 1621
HpyF3I CTNAG 3 cut(s) 6, 442, 1620
HpySE526I ACGT 6 cut(s) 52, 677, 771, 939, 1628, 1640
Hsp92I GRCGYC 2 cut(s) 408, 502
HspAI GCGC 3 cut(s) 502, 563, 826
KasI GGCGCC 1 cut(s) 501
KspAI GTTAAC 2 cut(s) 756, 954
Kzo9I GATC 6 cut(s) 849, 1000, 1107, 1131, 1311, 1342
LmnI GCTCC 4 cut(s) 1077, 1329, 1534, 1542
Lsp1109I GCAGC 5 cut(s) 178, 206, 303, 367, 555
LweI GCATC 2 cut(s) 700, 1108
MaeI CTAG 9 cut(s) 78, 113, 272, 362, 452, 714, 1028, 1247, 1499
MaeII ACGT 6 cut(s) 52, 677, 771, 939, 1628, 1640
MaeIII GTNAC 3 cut(s) 257, 644, 1242
MalI GATC 6 cut(s) 851, 1002, 1109, 1133, 1313, 1344
MboI GATC 6 cut(s) 849, 1000, 1107, 1131, 1311, 1342
MboII GAAGA 8 cut(s) 154, 619, 880, 1126, 1139, 1178, 1350, 1467
MflI RGATCY 2 cut(s) 1131, 1342
MhlI GDGCHC 4 cut(s) 450, 789, 1092, 1539
MlsI TGGCCA 1 cut(s) 308
MluCI AATT 3 cut(s) 144, 412, 1475
MluNI TGGCCA 1 cut(s) 308
Mly113I GGCGCC 1 cut(s) 502
MlyI GAGTC 1 cut(s) 142
MmeI TCCRAC 1 cut(s) 322
Mox20I TGGCCA 1 cut(s) 308
MscI TGGCCA 1 cut(s) 308
MseI TTAA 6 cut(s) 59, 755, 768, 830, 953, 1527
Msp20I TGGCCA 1 cut(s) 308
MspA1I CMGCKG 1 cut(s) 568
MspI CCGG 4 cut(s) 248, 759, 1414, 1466
MspR9I CCNGG 6 cut(s) 231, 437, 473, 760, 1415, 1467
MvaI CCWGG 3 cut(s) 231, 437, 473
MvnI CGCG 2 cut(s) 419, 1309
MwoI GCNNNNNNNGC 6 cut(s) 220, 416, 468, 1096, 1480, 1621
NarI GGCGCC 1 cut(s) 502
NciI CCSGG 3 cut(s) 760, 1415, 1467
NdeI CATATG 1 cut(s) 267
NdeII GATC 6 cut(s) 849, 1000, 1107, 1131, 1311, 1342
NlaIV GGNNCC 4 cut(s) 322, 493, 503, 1079
NmeAIII GCCGAG 1 cut(s) 1111
NmuCI GTSAC 2 cut(s) 257, 644
NruI TCGCGA 1 cut(s) 419
NspI RCATGY 3 cut(s) 42, 90, 1573
PaeR7I CTCGAG 1 cut(s) 527
PagI TCATGA 3 cut(s) 403, 1110, 1392
PceI AGGCCT 1 cut(s) 440
PciI ACATGT 2 cut(s) 86, 1569
PcsI WCGNNNNNNNCGW 2 cut(s) 962, 1064
PfeI GAWTC 4 cut(s) 22, 1117, 1169, 1259
PflMI CCANNNNNTGG 2 cut(s) 304, 1141
PfoI TCCNGGA 1 cut(s) 229
PinAI ACCGGT 1 cut(s) 247
PkrI GCNGC 7 cut(s) 193, 196, 293, 382, 567, 570, 1045
PleI GAGTC 1 cut(s) 141
PluTI GGCGCC 1 cut(s) 505
PpsI GAGTC 1 cut(s) 141
Ppu21I YACGTR 1 cut(s) 1641
PscI ACATGT 2 cut(s) 86, 1569
PshBI ATTAAT 1 cut(s) 59
Psp124BI GAGCTC 2 cut(s) 1092, 1539
Psp6I CCWGG 3 cut(s) 229, 435, 471
PspEI GGTNACC 1 cut(s) 257
PspGI CCWGG 3 cut(s) 229, 435, 471
PspN4I GGNNCC 4 cut(s) 322, 493, 503, 1079
PspPI GGNCC 6 cut(s) 233, 320, 469, 491, 797, 1153
PspXI VCTCGAGB 1 cut(s) 527
PstI CTGCAG 3 cut(s) 34, 196, 385
PstNI CAGNNNCTG 1 cut(s) 604
PsuI RGATCY 2 cut(s) 1131, 1342
RruI TCGCGA 1 cut(s) 419
RsaI GTAC 5 cut(s) 351, 488, 523, 990, 1631
RsaNI GTAC 5 cut(s) 350, 487, 522, 989, 1630
SacI GAGCTC 2 cut(s) 1092, 1539
SaqAI TTAA 6 cut(s) 59, 755, 768, 830, 953, 1527
SatI GCNGC 7 cut(s) 192, 195, 292, 381, 566, 569, 1044
Sau3AI GATC 6 cut(s) 849, 1000, 1107, 1131, 1311, 1342
Sau96I GGNCC 6 cut(s) 233, 320, 469, 491, 797, 1153
SchI GAGTC 1 cut(s) 142
ScrFI CCNGG 6 cut(s) 231, 437, 473, 760, 1415, 1467
SduI GDGCHC 4 cut(s) 450, 789, 1092, 1539
SfaNI GCATC 2 cut(s) 700, 1108
SfcI CTRYAG 3 cut(s) 30, 192, 381
SfoI GGCGCC 1 cut(s) 503
Sfr274I CTCGAG 1 cut(s) 527
SinI GGWCC 4 cut(s) 233, 320, 797, 1153
SlaI CTCGAG 1 cut(s) 527
SmlI CTYRAG 4 cut(s) 527, 1093, 1195, 1557
SmoI CTYRAG 4 cut(s) 527, 1093, 1195, 1557
SnaBI TACGTA 1 cut(s) 1641
SpeI ACTAGT 3 cut(s) 361, 1246, 1498
Sse9I AATT 3 cut(s) 144, 412, 1475
SseBI AGGCCT 1 cut(s) 440
SsiI CCGC 4 cut(s) 551, 566, 1043, 1309
SspDI GGCGCC 1 cut(s) 501
SspMI CTAG 9 cut(s) 78, 113, 272, 362, 452, 714, 1028, 1247, 1499
SstI GAGCTC 2 cut(s) 1092, 1539
StuI AGGCCT 1 cut(s) 440
StyD4I CCNGG 6 cut(s) 229, 435, 471, 758, 1413, 1465
StyI CCWWGG 2 cut(s) 340, 1103
TaaI ACNGT 7 cut(s) 433, 591, 966, 1242, 1492, 1596, 1634
TaiI ACGT 6 cut(s) 55, 680, 774, 942, 1631, 1643
TaqI TCGA 7 cut(s) 528, 545, 1003, 1067, 1266, 1281, 1495
TaqII GACCGA 2 cut(s) 785, 1170
TasI AATT 3 cut(s) 144, 412, 1475
TatI WGTACW 1 cut(s) 988
TauI GCSGC 2 cut(s) 568, 1046
TfiI GAWTC 4 cut(s) 22, 1117, 1169, 1259
Tru1I TTAA 6 cut(s) 59, 755, 768, 830, 953, 1527
Tru9I TTAA 6 cut(s) 59, 755, 768, 830, 953, 1527
TscAI CASTG 2 cut(s) 49, 1599
TseFI GTSAC 2 cut(s) 257, 644
TseI GCWGC 5 cut(s) 191, 194, 291, 380, 568
Tsp45I GTSAC 2 cut(s) 257, 644
TspDTI ATGAA 7 cut(s) 254, 900, 1127, 1272, 1381, 1409, 1508
TspRI CASTG 2 cut(s) 49, 1599
Van91I CCANNNNNTGG 2 cut(s) 304, 1141
VpaK11BI GGWCC 4 cut(s) 233, 320, 797, 1153
VspI ATTAAT 1 cut(s) 59
XceI RCATGY 3 cut(s) 42, 90, 1573
XcmI CCANNNNNNNNNTGG 1 cut(s) 1142
XhoI CTCGAG 1 cut(s) 527
XmiI GTMKAC 1 cut(s) 936
XspI CTAG 9 cut(s) 78, 113, 272, 362, 452, 714, 1028, 1247, 1499
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.