RLG00000004937

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0000282
Physical Location & Seq
Reverse (-)
63837607 .. 63843318
5712 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000004937

Sequence Viewer

Length: 1515 bp
ATGAGTCCTTCAACAATGACCATCCTTCTCCTCTTCGTTGCCCTCATATGGTTTCTCATCGCTGCAGCCTTCTCAAAACCAAAGCACAAGAAGCTGCCTCCTGGACCTTTGGCACTACCGGTCATTGGTCACCTTCATATGCTAGGCAATCTCCCACATCGCAGCCTCCAACACTTGGCCAAAAAATATGGACCCATAATGTCTATTCGTCTTGGGAATGTACCTACCATACTAGTCTCGTCCCCGAAAGCTGCAGAGCTATTCCTCAAAACCCATGACGCCAATTTCGCGAGCAGGCCGAGAGTCCAGGCCTCAGAGCACCTAGCTTACGGCACAATGGGCCTGGCGTTTGCCGAGTACGGCCCCTATTGGCGCCATGTGAGGAAGCTCTGCACCCTCGAGCTTTTTTGTCCCTCGAAAGCAGAGGCTTTTGCACCGCTGCGAAGGGAGGAGATAGGGCTGTTGGTGCAGTCGCTGAAGAAAGCTGGAGAGGGAGGTGAGGTGGTGGATGTTAGTGACAAGATTTATGGCGTTGGTGAGGATATAACGTATAGGATGATATTCGGTTGCATAAAGGATGATGCTAGGTTTGGTTTGAAGGAGATTGTTCACGAAATTTCATTGTTAACCGGAGCATTTAATGCAGCTGATTTTGTGCCCTTCCTCGGTCCATTTGATTTTCAGGGATTGACCAAGCGCTTAAAGAAAGTTAGCAAGACGATTGATCGACTCCTGGAGAAGATAATAGACGAGCATGAACAAGTTGCCGACACTAGGAGTGGGAAACAAGGCCATGAGGACTTTGTAGACGTGTTGCTTTCGTTAACGAACCAACCGTTGAGCCCGAATGATGAGCAAGTACACTTCCTTGATCGAACAAATGTGAAAGCCATCTTACTAGACATCATAACGGCGGCTTTTGATACTTCGGCCACATCAATTGTTTGGAGCCTTGCCGAGCTCTTGAGGCATCCAAGGATCATGAAGAATCTCCAAGAAGAGATCCAAAGTGTGGTTGGTATCGACCGAATGGTGGAAGAATCTGATTTGCCCAAGCTGGATTACTTGAGTATGGTGGTGAAGGAGAGCTTCAGACTACACCCAGTTGCACCGTTACTAGTCCCACATGAATCCATCGAGGACATTACAATCGATGGATATGACATACCCAAGAAGTCGCGGATCATAGTAAACATCTGGAGCATTGGAAGAGATCCTAATGTTTGGTCGGAAAATGTTGAGGAGTTTTATCCTGAAAGGTTCATGAACAGCAATATAGACCTCCGGGGACATGACTTTCAGCTCATCCCATTTGGGTCTGGGCGAAGAGGGTGTCCGGGTATGCAATTAGGGCTAACCACAGTTCGGCTAGTTCTGGCACAGCTGGTGCATTGCTTTAACTGGGAGCTCCCAAATGGCTTGCTACCTCAAGACTTGGACATGTCCGAGGATTTCGGCTTATCACTGTCAAAAGCCAAACACTTGCTTACTAAGCCAACGTACCGTCTTATGTAG

Protein Analysis

505

Amino Acids

56.62

Weight (kDa)

6.53

Isoelectric Point (pI)

39.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 33 - 491 9.6e-103 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000282)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g12350 FvH4_5g12380 FvH4_5g12392 FvH4_5g12400 FvH4_5g12430
malus_domestica MD06G1213300.v1.1 MD06G1213500.v1.1 MD06G1213700.v1.1 MD06G1213800.v1.1 MD06G1213900.v1.1 MD06G1214000.v1.1 MD06G1214400.v1.1 MD14G1224300.v1.1 MD14G1224400.v1.1 MD14G1224600.v1.1 MD14G1224700.v1.1 MD14G1224800.v1.1 MD14G1224900.v1.1 MD14G1225100.v1.1 MD14G1225400.v1.1
prunus_persica Prupe.5G219700_v2.0.a1 Prupe.5G219800_v2.0.a1 Prupe.5G219900_v2.0.a1 Prupe.8G078500_v2.0.a1
pyrus_communis pycom06g19100 pycom14g18590 pycom14g18600 pycom14g18610 pycom14g18660
rosa_chinensis RchiOBHm_Chr0c12g0499591 RchiOBHm_Chr2g0140591 RchiOBHm_Chr2g0140631 RchiOBHm_Chr2g0140641 RchiOBHm_Chr2g0140651 RchiOBHm_Chr4g0417991 RchiOBHm_Chr4g0418001 RchiOBHm_Chr4g0418101 RchiOBHm_Chr4g0418111 RchiOBHm_Chr4g0418121 RchiOBHm_Chr6g0280191 RchiOBHm_Chr6g0280241 RchiOBHm_Chr6g0280251 RchiOBHm_Chr7g0185031 RchiOBHm_Chr7g0185051 RchiOBHm_Chr7g0185081 RchiOBHm_Chr7g0185091 RchiOBHm_Chr7g0185111 RchiOBHm_Chr7g0185131 RchiOBHm_Chr7g0185151 RchiOBHm_Chr7g0185161 RchiOBHm_Chr7g0185191 RchiOBHm_Chr7g0222431
rosa_laevigata RLG00000002061 RLG00000004934 RLG00000004935 RLG00000004937 RLG00000013071 RLG00000013077 RLG00000019621 RLG00000019881 RLG00000019884 RLG00000019887 RLG00000019888 RLG00000028839
rosa_multiflora Rmu_co8014370.1_g000001 Rmu_co8334683.1_g000001 Rmu_co8440383.1_g000001 Rmu_sc0002826.1_g000008 Rmu_sc0002826.1_g000009 Rmu_sc0002826.1_g000030 Rmu_sc0004483.1_g000005 Rmu_sc0004638.1_g000003 Rmu_sc0004638.1_g000004 Rmu_sc0005756.1_g000003 Rmu_sc0006493.1_g000023 Rmu_sc0006493.1_g000024 Rmu_sc0007137.1_g000017 Rmu_sc0008496.1_g000022 Rmu_sc0008769.1_g000003 Rmu_sc0013612.1_g000026 Rmu_sc0013864.1_g000001 Rmu_sc0013864.1_g000007 Rmu_sc0013864.1_g000008 Rmu_sc0030128.1_g000001 Rmu_sc0041660.1_g000001 Rmu_ssc0000289.1_g000001
rosa_roxburghii Rroxscaffold_2G00104640 Rroxscaffold_3G00253970 Rroxscaffold_3G00269170 Rroxscaffold_3G00269200 Rroxscaffold_3G00269210 Rroxscaffold_3G00269220 Rroxscaffold_3G00269230 Rroxscaffold_7G00188320
rosa_rugosa Rorug01G0052300 Rorug05G0212000 Rorug06G0130700 Rorug06G0131100 Rorug06G0466200 Rorug06G0466300 Rorug06G0466400 Rorug06G0466500 Rorug06G0466700 Rorug07G0208500 Rorug07G0294600
rosa_samantha Rh4AG137600 Rh6DG237300 Rh6DG237800 Rh7AG076200 Rh7AG076300 Rh7AG076500 Rh7AG076600 Rh7AG350700 Rh7BG070800 Rh7BG070900 Rh7BG071000 Rh7BG071100
rosa_wichuraiana Rw6G020990 Rw6G021010 Rw7G005900 Rw7G005910 Rw7G005920 Rw7G005940 Rw7G005950 Rw7G005970 Rw7G029600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 372
AccB7I CCANNNNNTGG 2 cut(s) 175, 1012
AccI GTMKAC 1 cut(s) 807
AccII CGCG 2 cut(s) 290, 1180
AciI CCGC 3 cut(s) 437, 914, 1180
AclWI GGATC 4 cut(s) 986, 997, 1190, 1208
AcoI YGGCCR 2 cut(s) 177, 930
AcsI RAATTY 1 cut(s) 615
AcuI CTGAAG 2 cut(s) 497, 1075
AcyI GRCGYC 2 cut(s) 279, 373
AfaI GTAC 4 cut(s) 222, 359, 861, 1502
AfeI AGCGCT 1 cut(s) 698
AfiI CCNNNNNNNGG 8 cut(s) 48, 125, 175, 665, 774, 1012, 1033, 1365
AflIII ACRYGT 2 cut(s) 810, 1440
AgeI ACCGGT 1 cut(s) 118
AgsI TTSAA 2 cut(s) 12, 598
AhlI ACTAGT 2 cut(s) 232, 1117
AjiI CACGTC 1 cut(s) 811
AjnI CCWGG 4 cut(s) 100, 306, 342, 732
Alw21I GWGCWC 3 cut(s) 321, 963, 1410
Alw26I GTCTC 1 cut(s) 241
AlwI GGATC 4 cut(s) 986, 997, 1190, 1208
AlwNI CAGNNNCTG 1 cut(s) 475
Ama87I CYCGRG 1 cut(s) 398
Aor51HI AGCGCT 1 cut(s) 698
AoxI GGCC 7 cut(s) 177, 296, 309, 340, 361, 790, 930
ApeKI GCWGC 7 cut(s) 62, 65, 94, 162, 251, 439, 644
ApoI RAATTY 1 cut(s) 615
ArsI GACNNNNNNTTYG 4 cut(s) 269, 301, 1318, 1350
AsiGI ACCGGT 1 cut(s) 118
AspLEI GCGC 2 cut(s) 375, 699
AspS9I GGNCC 5 cut(s) 104, 191, 340, 362, 668
AsuC2I CCSGG 2 cut(s) 1286, 1338
AsuHPI GGTGA 4 cut(s) 122, 509, 548, 1090
AvaI CYCGRG 1 cut(s) 398
AvaII GGWCC 3 cut(s) 104, 191, 668
BaeGI GKGCMC 1 cut(s) 660
BalI TGGCCA 1 cut(s) 179
BanI GGYRCC 1 cut(s) 372
BanII GRGCYC 3 cut(s) 845, 963, 1410
Bbv12I GWGCWC 3 cut(s) 321, 963, 1410
BbvI GCAGC 7 cut(s) 49, 77, 81, 174, 238, 426, 656
BccI CCATC 4 cut(s) 29, 899, 1142, 1148
BceAI ACGGC 3 cut(s) 346, 376, 927
BciT130I CCWGG 4 cut(s) 102, 308, 344, 734
BcnI CCSGG 2 cut(s) 1286, 1338
BcoDI GTCTC 1 cut(s) 241
BcuI ACTAGT 2 cut(s) 232, 1117
BfaI CTAG 8 cut(s) 143, 233, 323, 585, 774, 899, 1118, 1370
BfmI CTRYAG 2 cut(s) 63, 252
BfoI RGCGCY 2 cut(s) 376, 700
BisI GCNGC 8 cut(s) 63, 66, 95, 163, 252, 440, 645, 915
BlsI GCNGC 8 cut(s) 64, 67, 96, 164, 253, 441, 646, 916
Bme1390I CCNGG 6 cut(s) 102, 308, 344, 734, 1286, 1338
Bme18I GGWCC 3 cut(s) 104, 191, 668
BmeT110I CYCGRG 1 cut(s) 398
BmgBI CACGTC 1 cut(s) 811
BmgT120I GGNCC 5 cut(s) 104, 191, 340, 362, 668
BmiI GGNNCC 4 cut(s) 193, 364, 374, 950
BmrFI CCNGG 6 cut(s) 102, 308, 344, 734, 1286, 1338
BmrI ACTGGG 2 cut(s) 1097, 1411
BmsI GCATC 2 cut(s) 571, 979
BmuI ACTGGG 2 cut(s) 1097, 1411
BpmI CTGGAG 3 cut(s) 507, 755, 1219
BpuEI CTTGAG 3 cut(s) 985, 1087, 1413
BpuMI CCSGG 2 cut(s) 1286, 1338
Bsa29I ATCGAT 1 cut(s) 1152
BsaHI GRCGYC 2 cut(s) 279, 373
BsaJI CCNNGG 4 cut(s) 664, 974, 1285, 1446
BsaWI WCCGGW 2 cut(s) 118, 629
BsaXI ACNNNNNCTCC 2 cut(s) 486, 516
Bsc4I CCNNNNNNNGG 8 cut(s) 48, 125, 175, 665, 774, 1012, 1033, 1365
Bse118I RCCGGY 1 cut(s) 118
Bse1I ACTGG 2 cut(s) 1103, 1406
Bse3DI GCAATG 1 cut(s) 1390
BseBI CCWGG 4 cut(s) 102, 308, 344, 734
BseCI ATCGAT 1 cut(s) 1152
BseDI CCNNGG 4 cut(s) 664, 974, 1285, 1446
BseGI GGATG 6 cut(s) 21, 514, 561, 583, 970, 1305
BseLI CCNNNNNNNGG 8 cut(s) 48, 125, 175, 665, 774, 1012, 1033, 1365
BseMI GCAATG 1 cut(s) 1390
BseMII CTCAG 1 cut(s) 327
BseNI ACTGG 2 cut(s) 1103, 1406
BseRI GAGGAG 3 cut(s) 20, 464, 1256
BseSI GKGCMC 1 cut(s) 660
BseXI GCAGC 7 cut(s) 49, 77, 81, 174, 238, 426, 656
BsgI GTGCAG 2 cut(s) 376, 488
Bsh1236I CGCG 2 cut(s) 290, 1180
Bsh1285I CGRYCG 1 cut(s) 1027
BshFI GGCC 7 cut(s) 179, 298, 311, 342, 363, 792, 932
BshNI GGYRCC 1 cut(s) 372
BshTI ACCGGT 1 cut(s) 118
BshVI ATCGAT 1 cut(s) 1152
BsiEI CGRYCG 1 cut(s) 1027
BsiHKAI GWGCWC 3 cut(s) 321, 963, 1410
BsiHKCI CYCGRG 1 cut(s) 398
BsiSI CCGG 4 cut(s) 119, 630, 1285, 1337
BslFI GGGAC 4 cut(s) 226, 396, 1106, 1302
BslI CCNNNNNNNGG 8 cut(s) 48, 125, 175, 665, 774, 1012, 1033, 1365
BsmAI GTCTC 1 cut(s) 241
BsmFI GGGAC 4 cut(s) 226, 396, 1106, 1302
BsnI GGCC 7 cut(s) 179, 298, 311, 342, 363, 792, 932
BsoBI CYCGRG 1 cut(s) 398
Bsp1286I GDGCHC 5 cut(s) 321, 660, 845, 963, 1410
Bsp143I GATC 6 cut(s) 724, 871, 978, 1002, 1182, 1213
Bsp68I TCGCGA 1 cut(s) 290
BspACI CCGC 3 cut(s) 437, 914, 1180
BspANI GGCC 7 cut(s) 179, 298, 311, 342, 363, 792, 932
BspCNI CTCAG 1 cut(s) 326
BspDI ATCGAT 1 cut(s) 1152
BspFNI CGCG 2 cut(s) 290, 1180
BspHI TCATGA 2 cut(s) 981, 1263
BspLI GGNNCC 4 cut(s) 193, 364, 374, 950
BspMAI CTGCAG 2 cut(s) 67, 256
BspPI GGATC 4 cut(s) 986, 997, 1190, 1208
BspT107I GGYRCC 1 cut(s) 372
BsrDI GCAATG 1 cut(s) 1390
BsrFI RCCGGY 1 cut(s) 118
BsrI ACTGG 2 cut(s) 1103, 1406
BssAI RCCGGY 1 cut(s) 118
BssECI CCNNGG 4 cut(s) 664, 974, 1285, 1446
BssMI GATC 6 cut(s) 724, 871, 978, 1002, 1182, 1213
BssNI GRCGYC 2 cut(s) 279, 373
BssT1I CCWWGG 1 cut(s) 974
Bst2UI CCWGG 4 cut(s) 102, 308, 344, 734
Bst4CI ACNGT 5 cut(s) 837, 1113, 1363, 1467, 1505
Bst6I CTCTTC 4 cut(s) 38, 993, 1204, 1321
BstACI GRCGYC 2 cut(s) 279, 373
BstAPI GCANNNNNTGC 1 cut(s) 641
BstC8I GCNNGC 3 cut(s) 292, 296, 1421
BstDEI CTNAG 2 cut(s) 313, 1491
BstEII GGTNACC 1 cut(s) 128
BstF5I GGATG 6 cut(s) 21, 514, 561, 583, 970, 1305
BstFNI CGCG 2 cut(s) 290, 1180
BstH2I RGCGCY 2 cut(s) 376, 700
BstHHI GCGC 2 cut(s) 375, 699
BstKTI GATC 6 cut(s) 727, 874, 981, 1005, 1185, 1216
BstMAI GTCTC 1 cut(s) 241
BstMBI GATC 6 cut(s) 724, 871, 978, 1002, 1182, 1213
BstMCI CGRYCG 1 cut(s) 1027
BstMWI GCNNNNNNNGC 8 cut(s) 91, 287, 339, 466, 641, 967, 1351, 1492
BstNI CCWGG 4 cut(s) 102, 308, 344, 734
BstNSI RCATGY 1 cut(s) 1444
BstPI GGTNACC 1 cut(s) 128
BstSCI CCNGG 6 cut(s) 100, 306, 342, 732, 1284, 1336
BstSFI CTRYAG 2 cut(s) 63, 252
BstSLI GKGCMC 1 cut(s) 660
BstUI CGCG 2 cut(s) 290, 1180
BstV1I GCAGC 7 cut(s) 49, 77, 81, 174, 238, 426, 656
BstX2I RGATCY 2 cut(s) 1002, 1213
BstYI RGATCY 2 cut(s) 1002, 1213
Bsu15I ATCGAT 1 cut(s) 1152
BsuRI GGCC 7 cut(s) 179, 298, 311, 342, 363, 792, 932
BsuTUI ATCGAT 1 cut(s) 1152
BtgZI GCGATG 2 cut(s) 43, 143
BtrI CACGTC 1 cut(s) 811
BtsCI GGATG 6 cut(s) 21, 514, 561, 583, 970, 1305
BtsIMutI CAGTG 1 cut(s) 1463
BtuMI TCGCGA 1 cut(s) 290
Cac8I GCNNGC 3 cut(s) 292, 296, 1421
CaiI CAGNNNCTG 1 cut(s) 475
CciI TCATGA 2 cut(s) 981, 1263
CfoI GCGC 2 cut(s) 375, 699
Cfr10I RCCGGY 1 cut(s) 118
Cfr13I GGNCC 5 cut(s) 104, 191, 340, 362, 668
ClaI ATCGAT 1 cut(s) 1152
CseI GACGC 1 cut(s) 287
Csp6I GTAC 4 cut(s) 221, 358, 860, 1501
CspAI ACCGGT 1 cut(s) 118
CspCI CAANNNNNGTGG 2 cut(s) 922, 957
CviAII CATG 9 cut(s) 275, 377, 755, 794, 982, 1127, 1264, 1292, 1441
CviQI GTAC 4 cut(s) 221, 358, 860, 1501
DdeI CTNAG 2 cut(s) 313, 1491
DinI GGCGCC 1 cut(s) 374
DpnI GATC 6 cut(s) 726, 873, 980, 1004, 1184, 1215
DpnII GATC 6 cut(s) 724, 871, 978, 1002, 1182, 1213
EaeI YGGCCR 2 cut(s) 177, 930
Eam1104I CTCTTC 4 cut(s) 38, 993, 1204, 1321
EarI CTCTTC 4 cut(s) 38, 993, 1204, 1321
Ecl136II GAGCTC 2 cut(s) 961, 1408
Eco130I CCWWGG 1 cut(s) 974
Eco147I AGGCCT 1 cut(s) 311
Eco24I GRGCYC 3 cut(s) 845, 963, 1410
Eco47I GGWCC 3 cut(s) 104, 191, 668
Eco47III AGCGCT 1 cut(s) 698
Eco53kI GAGCTC 2 cut(s) 961, 1408
Eco57I CTGAAG 2 cut(s) 497, 1075
Eco88I CYCGRG 1 cut(s) 398
Eco91I GGTNACC 1 cut(s) 128
EcoICRI GAGCTC 2 cut(s) 961, 1408
EcoO65I GGTNACC 1 cut(s) 128
EcoRII CCWGG 4 cut(s) 100, 306, 342, 732
EcoT14I CCWWGG 1 cut(s) 974
EcoT38I GRGCYC 3 cut(s) 845, 963, 1410
EgeI GGCGCC 1 cut(s) 374
EheI GGCGCC 1 cut(s) 374
ErhI CCWWGG 1 cut(s) 974
FaeI CATG 9 cut(s) 278, 380, 758, 797, 985, 1130, 1267, 1295, 1444
FalI AAGNNNNNCTT 2 cut(s) 1073, 1105
FaqI GGGAC 4 cut(s) 226, 396, 1106, 1302
FatI CATG 9 cut(s) 274, 376, 754, 793, 981, 1126, 1263, 1291, 1440
FauNDI CATATG 2 cut(s) 47, 138
FblI GTMKAC 1 cut(s) 807
Fnu4HI GCNGC 8 cut(s) 63, 66, 95, 163, 252, 440, 645, 915
FokI GGATG 6 cut(s) 8, 521, 568, 590, 957, 1292
FriOI GRGCYC 3 cut(s) 845, 963, 1410
Fsp4HI GCNGC 8 cut(s) 63, 66, 95, 163, 252, 440, 645, 915
FspBI CTAG 8 cut(s) 143, 233, 323, 585, 774, 899, 1118, 1370
GlaI GCGC 2 cut(s) 374, 698
GluI GCNGC 8 cut(s) 63, 66, 95, 163, 252, 440, 645, 915
GsuI CTGGAG 3 cut(s) 507, 755, 1219
HaeII RGCGCY 2 cut(s) 376, 700
HaeIII GGCC 7 cut(s) 179, 298, 311, 342, 363, 792, 932
HapII CCGG 4 cut(s) 119, 630, 1285, 1337
HgaI GACGC 1 cut(s) 287
HhaI GCGC 2 cut(s) 375, 699
Hin1I GRCGYC 2 cut(s) 279, 373
Hin1II CATG 9 cut(s) 278, 380, 758, 797, 985, 1130, 1267, 1295, 1444
Hin6I GCGC 2 cut(s) 373, 697
HinP1I GCGC 2 cut(s) 373, 697
HincII GTYRAC 2 cut(s) 627, 825
HindII GTYRAC 2 cut(s) 627, 825
HinfI GANTC 6 cut(s) 4, 303, 729, 988, 1040, 1130
HpaI GTTAAC 2 cut(s) 627, 825
HpaII CCGG 4 cut(s) 119, 630, 1285, 1337
HphI GGTGA 4 cut(s) 122, 509, 548, 1090
Hpy166II GTNNAC 6 cut(s) 610, 627, 808, 825, 862, 1192
Hpy188I TCNGA 5 cut(s) 316, 1045, 1094, 1231, 1447
Hpy188III TCNNGA 8 cut(s) 289, 611, 964, 982, 1198, 1253, 1264, 1430
Hpy8I GTNNAC 6 cut(s) 610, 627, 808, 825, 862, 1192
HpyAV CCTTC 8 cut(s) 18, 35, 79, 143, 438, 592, 670, 1075
HpyCH4III ACNGT 5 cut(s) 837, 1113, 1363, 1467, 1505
HpyCH4IV ACGT 3 cut(s) 548, 810, 1499
HpyF10VI GCNNNNNNNGC 8 cut(s) 91, 287, 339, 466, 641, 967, 1351, 1492
HpyF3I CTNAG 2 cut(s) 313, 1491
HpySE526I ACGT 3 cut(s) 548, 810, 1499
Hsp92I GRCGYC 2 cut(s) 279, 373
Hsp92II CATG 9 cut(s) 278, 380, 758, 797, 985, 1130, 1267, 1295, 1444
HspAI GCGC 2 cut(s) 373, 697
KasI GGCGCC 1 cut(s) 372
KspAI GTTAAC 2 cut(s) 627, 825
Kzo9I GATC 6 cut(s) 724, 871, 978, 1002, 1182, 1213
LmnI GCTCC 5 cut(s) 632, 948, 1200, 1405, 1413
Lsp1109I GCAGC 7 cut(s) 49, 77, 81, 174, 238, 426, 656
LweI GCATC 2 cut(s) 571, 979
MaeI CTAG 8 cut(s) 143, 233, 323, 585, 774, 899, 1118, 1370
MaeII ACGT 3 cut(s) 548, 810, 1499
MaeIII GTNAC 3 cut(s) 128, 515, 1113
MalI GATC 6 cut(s) 726, 873, 980, 1004, 1184, 1215
MboI GATC 6 cut(s) 724, 871, 978, 1002, 1182, 1213
MboII GAAGA 8 cut(s) 25, 490, 751, 997, 1010, 1049, 1221, 1338
MfeI CAATTG 1 cut(s) 939
MflI RGATCY 2 cut(s) 1002, 1213
MhlI GDGCHC 5 cut(s) 321, 660, 845, 963, 1410
MlsI TGGCCA 1 cut(s) 179
MluCI AATT 4 cut(s) 283, 615, 939, 1346
MluNI TGGCCA 1 cut(s) 179
Mly113I GGCGCC 1 cut(s) 373
MlyI GAGTC 3 cut(s) 13, 312, 723
MmeI TCCRAC 2 cut(s) 193, 1209
Mox20I TGGCCA 1 cut(s) 179
MscI TGGCCA 1 cut(s) 179
MseI TTAA 5 cut(s) 626, 639, 701, 824, 1398
Msp20I TGGCCA 1 cut(s) 179
MspA1I CMGCKG 3 cut(s) 439, 647, 1384
MspI CCGG 4 cut(s) 119, 630, 1285, 1337
MspR9I CCNGG 6 cut(s) 102, 308, 344, 734, 1286, 1338
MunI CAATTG 1 cut(s) 939
MvaI CCWGG 4 cut(s) 102, 308, 344, 734
MvnI CGCG 2 cut(s) 290, 1180
MwoI GCNNNNNNNGC 8 cut(s) 91, 287, 339, 466, 641, 967, 1351, 1492
NarI GGCGCC 1 cut(s) 373
NciI CCSGG 2 cut(s) 1286, 1338
NdeI CATATG 2 cut(s) 47, 138
NdeII GATC 6 cut(s) 724, 871, 978, 1002, 1182, 1213
NlaIII CATG 9 cut(s) 278, 380, 758, 797, 985, 1130, 1267, 1295, 1444
NlaIV GGNNCC 4 cut(s) 193, 364, 374, 950
NmeAIII GCCGAG 3 cut(s) 324, 379, 982
NmuCI GTSAC 2 cut(s) 128, 515
NruI TCGCGA 1 cut(s) 290
NspI RCATGY 1 cut(s) 1444
PaeR7I CTCGAG 1 cut(s) 398
PagI TCATGA 2 cut(s) 981, 1263
PceI AGGCCT 1 cut(s) 311
PciI ACATGT 1 cut(s) 1440
PcsI WCGNNNNNNNCGW 1 cut(s) 833
PfeI GAWTC 3 cut(s) 988, 1040, 1130
PflMI CCANNNNNTGG 2 cut(s) 175, 1012
PfoI TCCNGGA 2 cut(s) 100, 732
PinAI ACCGGT 1 cut(s) 118
PkrI GCNGC 8 cut(s) 64, 67, 96, 164, 253, 441, 646, 916
PleI GAGTC 3 cut(s) 12, 311, 723
PluTI GGCGCC 1 cut(s) 376
PpsI GAGTC 3 cut(s) 12, 311, 723
PscI ACATGT 1 cut(s) 1440
Psp124BI GAGCTC 2 cut(s) 963, 1410
Psp6I CCWGG 4 cut(s) 100, 306, 342, 732
PspEI GGTNACC 1 cut(s) 128
PspGI CCWGG 4 cut(s) 100, 306, 342, 732
PspN4I GGNNCC 4 cut(s) 193, 364, 374, 950
PspPI GGNCC 5 cut(s) 104, 191, 340, 362, 668
PspXI VCTCGAGB 1 cut(s) 398
PstI CTGCAG 2 cut(s) 67, 256
PstNI CAGNNNCTG 1 cut(s) 475
PsuI RGATCY 2 cut(s) 1002, 1213
PvuII CAGCTG 2 cut(s) 647, 1384
RruI TCGCGA 1 cut(s) 290
RsaI GTAC 4 cut(s) 222, 359, 861, 1502
RsaNI GTAC 4 cut(s) 221, 358, 860, 1501
SacI GAGCTC 2 cut(s) 963, 1410
SaqAI TTAA 5 cut(s) 626, 639, 701, 824, 1398
SatI GCNGC 8 cut(s) 63, 66, 95, 163, 252, 440, 645, 915
Sau3AI GATC 6 cut(s) 724, 871, 978, 1002, 1182, 1213
Sau96I GGNCC 5 cut(s) 104, 191, 340, 362, 668
SchI GAGTC 3 cut(s) 13, 312, 723
ScrFI CCNGG 6 cut(s) 102, 308, 344, 734, 1286, 1338
SduI GDGCHC 5 cut(s) 321, 660, 845, 963, 1410
SfaNI GCATC 2 cut(s) 571, 979
SfcI CTRYAG 2 cut(s) 63, 252
SfoI GGCGCC 1 cut(s) 374
Sfr274I CTCGAG 1 cut(s) 398
SinI GGWCC 3 cut(s) 104, 191, 668
SlaI CTCGAG 1 cut(s) 398
SmlI CTYRAG 4 cut(s) 398, 964, 1066, 1428
SmoI CTYRAG 4 cut(s) 398, 964, 1066, 1428
SpeI ACTAGT 2 cut(s) 232, 1117
Sse9I AATT 4 cut(s) 283, 615, 939, 1346
SseBI AGGCCT 1 cut(s) 311
SsiI CCGC 3 cut(s) 437, 914, 1180
SspDI GGCGCC 1 cut(s) 372
SspMI CTAG 8 cut(s) 143, 233, 323, 585, 774, 899, 1118, 1370
SstI GAGCTC 2 cut(s) 963, 1410
StuI AGGCCT 1 cut(s) 311
StyD4I CCNGG 6 cut(s) 100, 306, 342, 732, 1284, 1336
StyI CCWWGG 1 cut(s) 974
TaaI ACNGT 5 cut(s) 837, 1113, 1363, 1467, 1505
TaiI ACGT 3 cut(s) 551, 813, 1502
TaqI TCGA 7 cut(s) 399, 416, 727, 874, 1023, 1137, 1152
TaqII GACCGA 2 cut(s) 656, 1041
TasI AATT 4 cut(s) 283, 615, 939, 1346
TatI WGTACW 1 cut(s) 859
TauI GCSGC 1 cut(s) 917
TfiI GAWTC 3 cut(s) 988, 1040, 1130
Tru1I TTAA 5 cut(s) 626, 639, 701, 824, 1398
Tru9I TTAA 5 cut(s) 626, 639, 701, 824, 1398
TscAI CASTG 1 cut(s) 1470
TseFI GTSAC 2 cut(s) 128, 515
TseI GCWGC 7 cut(s) 62, 65, 94, 162, 251, 439, 644
Tsp45I GTSAC 2 cut(s) 128, 515
TspDTI ATGAA 7 cut(s) 125, 609, 771, 998, 1143, 1252, 1280
TspRI CASTG 1 cut(s) 1470
Van91I CCANNNNNTGG 2 cut(s) 175, 1012
VpaK11BI GGWCC 3 cut(s) 104, 191, 668
XapI RAATTY 1 cut(s) 615
XceI RCATGY 1 cut(s) 1444
XcmI CCANNNNNNNNNTGG 1 cut(s) 1013
XhoI CTCGAG 1 cut(s) 398
XmiI GTMKAC 1 cut(s) 807
XspI CTAG 8 cut(s) 143, 233, 323, 585, 774, 899, 1118, 1370
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.