Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0000282

Physical Location & Seq

Genomic Coordinates Forward (+)

34714412 .. 34719017

Gene Structure 4606 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1214400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1875 bp

ATGTGTGTGTCTAATGTCCCATTTATCGATCTCATCCAGAAATTATATAAAATGAGTCCTTCAGAAATAGCCATCCTCATCCTTGTATTCCTCACATTCCTATGGTCTCTCATCCGCCTTATCAATGCCTCCTCAAGGCAAAGCCGAAAACTTCCTCCTGGCCCTACGGCACTGCCAATCATTGGCAATCTTCATATGCTAGGCGACCTCCCACACCGTAGCCTCCAAAACCTAGCAAAAAAATATGGACCTATAATGTCCATGCGTCTAGGGTTCATACCAACCATAGTGGTCTCATCTCCCAAAACAGCAGAGCTATTCCTCAAAACCCACGACACCATTTTCGCTAGCCGCCCCAAACTCCAAGCCTCCGAGTACATGGCCTACGGCACGAAGGCCATGGCCTTAACTGAATATGGCCCATATTGGCGCCGTATTCGGAAACTCTGCACGCTTCAACTTCTTTGTCCCTCGAAAATCGAGGGTTTTGCTCCGCTAAGAAGGGAGGAGGTAGGGTTGTTGGTGCAGTCCCTGAAGGTAGCTGCGGAGGCAGGCGAGGTGGTGGATTTTAGTGAGAAGGTCGGTGAGCTTGTTGAGGGAATCACGTATAGGATGGTGTTGGGGAGAAAAAATGATGATATGTTTGATCTCAAAGGGATTATTGAGGAAGCCCTATTCTTGACAGGAGCTTTCAACATTAGTGATTATGTGCCTTTCCTTAGTCCACTTGATCTTCAGGGATTGACCAAGCGCATGAAGAGAGTTAGCAAAACTGTCGACCAACTACTCGAAAAGATAATCCAGGATCATGAACAAGTTTCCAGAAGTGAGGTACAAGGCAATCATCATAAGGACTTTGTAGACGTGCTGCTTTCATCAATCCACCAACCATTAAACCCCAACGATGAGGAAGTCTACATGCTCGAGCGAACAAATGTCAAAGCCATTTTACTCGACATGATTGCGGGTGCCTTTGACACCTCAGCCACAGCGATTGTCTGGACCCTGGCCGAACTCTTGAGGCACCCGAAAGTAATGAAACGTCTCCAAGAAGAGCTCCAGAGTGTGATCGGGATGGATCGAATGGTGGAAGAAAGTGATTTGCCAAAGCTGGATTACTTGAGCATGGTGGTCAAGGAGAGCTTAAGGTTACACCCAGTTGCCCCATTACTAGTCCCTCATCAATCCATGGAGGACATTACGGTTGATGGATATCACATACCCAAGAAATCAAGAGTCTTTATCAATATTTGGACCATTGGGAGGGATCCAAGTGTATGGTCAGATAATGTGGAAGAATTCTACCCTGAAAGGTTTATGAATAATAACGTTGACCTACGTGGACATGACTTCCAGCTCATACCATTTGGGTCCGGTCGAAGAGGGTGCCCTGCTATGCAATTAGGGTTAACCACAGTTCGTCTTGTTCTGGGAAACTTGTTGCACTGCTTCAACTGGGAGCTCCCTAGTGGAATGCTACCTAAAGACTTGGACATGACTGAGAAATTCGGCTTATCGTTGTCGAAAGCCAAACACTTGCTTGCCATGCCGACCTGCCGCCTGTACAATGGAAACGGGACGGGACGGAACGGAATGCGACAGGACGGGACAGGACAGGACTGGACGGAACGGAGAGGAAGCAAAGATGCCCTCAGATGGAAACAAGGAGAAAGAAGAATGAGACAGAGAGATTATAATTTTGTGTTCCACGAATGTGGAACAAGTTGTTCCGGGGGGGTGAGGTGGAACGAAAATTCACCCAAAACTCGTCACGTGGAACAGCGCGTTCCACCTGTTTTAGGCGCACCAAACGTGGGACGGAACGTCTCGTCCCACTCTGTTCCGTCCCGTCCCATGTACCAAACGGTACCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004497 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0008150 GO:0008152 GO:0009058 GO:0012505 GO:0016020 GO:0016491 GO:0016705 GO:0016709 GO:0019748 GO:0031090 GO:0031984 GO:0042175 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0044550 GO:0055114 GO:0098827

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

p450

Protein Analysis

625

Amino Acids

70.69

Weight (kDa)

8.97

Isoelectric Point (pI)

55.67

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
p450	PF00067	52 - 511	3e-101	Cytochrome P450

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000282)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_5g12350 FvH4_5g12380 FvH4_5g12392 FvH4_5g12400 FvH4_5g12430
malus_domestica	MD06G1213300.v1.1 MD06G1213500.v1.1 MD06G1213700.v1.1 MD06G1213800.v1.1 MD06G1213900.v1.1 MD06G1214000.v1.1 MD06G1214400.v1.1 MD14G1224300.v1.1 MD14G1224400.v1.1 MD14G1224600.v1.1 MD14G1224700.v1.1 MD14G1224800.v1.1 MD14G1224900.v1.1 MD14G1225100.v1.1 MD14G1225400.v1.1
prunus_persica	Prupe.5G219700_v2.0.a1 Prupe.5G219800_v2.0.a1 Prupe.5G219900_v2.0.a1 Prupe.8G078500_v2.0.a1
pyrus_communis	pycom06g19100 pycom14g18590 pycom14g18600 pycom14g18610 pycom14g18660
rosa_chinensis	RchiOBHm_Chr0c12g0499591 RchiOBHm_Chr2g0140591 RchiOBHm_Chr2g0140631 RchiOBHm_Chr2g0140641 RchiOBHm_Chr2g0140651 RchiOBHm_Chr4g0417991 RchiOBHm_Chr4g0418001 RchiOBHm_Chr4g0418101 RchiOBHm_Chr4g0418111 RchiOBHm_Chr4g0418121 RchiOBHm_Chr6g0280191 RchiOBHm_Chr6g0280241 RchiOBHm_Chr6g0280251 RchiOBHm_Chr7g0185031 RchiOBHm_Chr7g0185051 RchiOBHm_Chr7g0185081 RchiOBHm_Chr7g0185091 RchiOBHm_Chr7g0185111 RchiOBHm_Chr7g0185131 RchiOBHm_Chr7g0185151 RchiOBHm_Chr7g0185161 RchiOBHm_Chr7g0185191 RchiOBHm_Chr7g0222431
rosa_laevigata	RLG00000002061 RLG00000004934 RLG00000004935 RLG00000004937 RLG00000013071 RLG00000013077 RLG00000019621 RLG00000019881 RLG00000019884 RLG00000019887 RLG00000019888 RLG00000028839
rosa_multiflora	Rmu_co8014370.1_g000001 Rmu_co8334683.1_g000001 Rmu_co8440383.1_g000001 Rmu_sc0002826.1_g000008 Rmu_sc0002826.1_g000009 Rmu_sc0002826.1_g000030 Rmu_sc0004483.1_g000005 Rmu_sc0004638.1_g000003 Rmu_sc0004638.1_g000004 Rmu_sc0005756.1_g000003 Rmu_sc0006493.1_g000023 Rmu_sc0006493.1_g000024 Rmu_sc0007137.1_g000017 Rmu_sc0008496.1_g000022 Rmu_sc0008769.1_g000003 Rmu_sc0013612.1_g000026 Rmu_sc0013864.1_g000001 Rmu_sc0013864.1_g000007 Rmu_sc0013864.1_g000008 Rmu_sc0030128.1_g000001 Rmu_sc0041660.1_g000001 Rmu_ssc0000289.1_g000001
rosa_roxburghii	Rroxscaffold_2G00104640 Rroxscaffold_3G00253970 Rroxscaffold_3G00269170 Rroxscaffold_3G00269200 Rroxscaffold_3G00269210 Rroxscaffold_3G00269220 Rroxscaffold_3G00269230 Rroxscaffold_7G00188320
rosa_rugosa	Rorug01G0052300 Rorug05G0212000 Rorug06G0130700 Rorug06G0131100 Rorug06G0466200 Rorug06G0466300 Rorug06G0466400 Rorug06G0466500 Rorug06G0466700 Rorug07G0208500 Rorug07G0294600
rosa_samantha	Rh4AG137600 Rh6DG237300 Rh6DG237800 Rh7AG076200 Rh7AG076300 Rh7AG076500 Rh7AG076600 Rh7AG350700 Rh7BG070800 Rh7BG070900 Rh7BG071000 Rh7BG071100
rosa_wichuraiana	Rw6G020990 Rw6G021010 Rw7G005900 Rw7G005910 Rw7G005920 Rw7G005940 Rw7G005950 Rw7G005970 Rw7G029600

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1695
Acc36I	ACCTGC	1 cut(s)	1562
Acc65I	GGTACC	1 cut(s)	1867
AccB1I	GGYRCC	5 cut(s)	429, 968, 1023, 1386, 1867
AccB7I	CCANNNNNTGG	1 cut(s)	182
AccI	GTMKAC	3 cut(s)	777, 861, 915
AccII	CGCG	1 cut(s)	1785
AciI	CCGC	6 cut(s)	115, 352, 494, 545, 965, 1558
AclI	AACGTT	1 cut(s)	1329
AclWI	GGATC	4 cut(s)	813, 1086, 1262, 1275
AcoI	YGGCCR	1 cut(s)	1008
AcsI	RAATTY	3 cut(s)	1298, 1505, 1753
AcuI	CTGAAG	3 cut(s)	45, 554, 719
AcvI	CACGTG	1 cut(s)	1774
AcyI	GRCGYC	1 cut(s)	430
AfaI	GTAC	5 cut(s)	377, 834, 1565, 1859, 1869
AfiI	CCNNNNNNNGG	6 cut(s)	135, 182, 1263, 1656, 1799, 1814
AflII	CTTAAG	1 cut(s)	1144
AgsI	TTSAA	3 cut(s)	458, 694, 1453
AhdI	GACNNNNNGTC	1 cut(s)	1823
AhlI	ACTAGT	1 cut(s)	1171
AjiI	CACGTC	1 cut(s)	865
AjnI	CCWGG	3 cut(s)	157, 801, 1005
AleI	CACNNNNGTG	1 cut(s)	1713
AloI	GAACNNNNNNTCC	2 cut(s)	1814, 1846
AluBI	AGCT	9 cut(s)	316, 542, 589, 689, 1057, 1111, 1143, 1357, 1462
AluI	AGCT	9 cut(s)	316, 542, 589, 689, 1057, 1111, 1143, 1357, 1462
Alw21I	GWGCWC	2 cut(s)	1059, 1464
Alw26I	GTCTC	5 cut(s)	111, 298, 1049, 1675, 1831
AlwI	GGATC	4 cut(s)	813, 1086, 1262, 1275
AlwNI	CAGNNNCTG	1 cut(s)	532
Ama87I	CYCGRG	1 cut(s)	923
AoxI	GGCC	6 cut(s)	160, 381, 396, 402, 418, 1008
ApeKI	GCWGC	2 cut(s)	542, 868
ApoI	RAATTY	3 cut(s)	1298, 1505, 1753
ArsI	GACNNNNNNTTYG	2 cut(s)	326, 358
Asp718I	GGTACC	1 cut(s)	1867
AspLEI	GCGC	4 cut(s)	432, 753, 1785, 1805
AspS9I	GGNCC	6 cut(s)	161, 248, 419, 1002, 1254, 1371
AsuC2I	CCSGG	1 cut(s)	1732
AsuHPI	GGTGA	3 cut(s)	596, 1749, 1750
AsuNHI	GCTAGC	1 cut(s)	347
AvaI	CYCGRG	1 cut(s)	923
AvaII	GGWCC	4 cut(s)	248, 1002, 1254, 1371
BaeGI	GKGCMC	1 cut(s)	1391
BamHI	GGATCC	1 cut(s)	1267
BanI	GGYRCC	5 cut(s)	429, 968, 1023, 1386, 1867
BanII	GRGCYC	2 cut(s)	1059, 1464
BbrPI	CACGTG	1 cut(s)	1774
Bbv12I	GWGCWC	2 cut(s)	1059, 1464
BbvCI	CCTCAGC	1 cut(s)	982
BbvI	GCAGC	2 cut(s)	529, 855
BccI	CCATC	5 cut(s)	80, 607, 1069, 1202, 1650
BceAI	ACGGC	3 cut(s)	183, 403, 417
BcgI	CGANNNNNNTGC	8 cut(s)	470, 504, 757, 791, 944, 978, 1368, 1402
BciT130I	CCWGG	3 cut(s)	159, 803, 1007
BcnI	CCSGG	1 cut(s)	1732
BcoDI	GTCTC	5 cut(s)	111, 298, 1049, 1675, 1831
BcuI	ACTAGT	1 cut(s)	1171
BfaI	CTAG	6 cut(s)	200, 233, 269, 348, 1172, 1467
BfoI	RGCGCY	1 cut(s)	433
BfrI	CTTAAG	1 cut(s)	1144
BfuAI	ACCTGC	1 cut(s)	1562
BisI	GCNGC	4 cut(s)	352, 543, 869, 1558
BlsI	GCNGC	4 cut(s)	353, 544, 870, 1559
Bme1390I	CCNGG	4 cut(s)	159, 803, 1007, 1732
Bme18I	GGWCC	4 cut(s)	248, 1002, 1254, 1371
BmeRI	GACNNNNNGTC	1 cut(s)	1823
BmeT110I	CYCGRG	1 cut(s)	923
BmgBI	CACGTC	1 cut(s)	865
BmgT120I	GGNCC	6 cut(s)	161, 248, 419, 1002, 1254, 1371
BmiI	GGNNCC	8 cut(s)	431, 970, 1004, 1025, 1269, 1372, 1388, 1869
BmrFI	CCNGG	4 cut(s)	159, 803, 1007, 1732
BmrI	ACTGGG	2 cut(s)	1151, 1465
BmsI	GCATC	1 cut(s)	1636
BmtI	GCTAGC	1 cut(s)	351
BmuI	ACTGGG	2 cut(s)	1151, 1465
BpmI	CTGGAG	1 cut(s)	1043
Bpu10I	CCTNAGC	1 cut(s)	982
BpuEI	CTTGAG	3 cut(s)	118, 1039, 1141
BpuMI	CCSGG	1 cut(s)	1732
Bsa29I	ATCGAT	1 cut(s)	27
BsaAI	YACGTR	3 cut(s)	606, 1340, 1774
BsaBI	GATNNNNATC	1 cut(s)	1212
BsaHI	GRCGYC	1 cut(s)	430
BsaI	GGTCTC	2 cut(s)	111, 298
BsaJI	CCNNGG	4 cut(s)	399, 1005, 1188, 1731
BsaWI	WCCGGW	1 cut(s)	1373
BsaXI	ACNNNNNCTCC	2 cut(s)	500, 530
Bsc4I	CCNNNNNNNGG	6 cut(s)	135, 182, 1263, 1656, 1799, 1814
Bse1I	ACTGG	3 cut(s)	1157, 1460, 1625
Bse8I	GATNNNNATC	1 cut(s)	1212
BseBI	CCWGG	3 cut(s)	159, 803, 1007
BseCI	ATCGAT	1 cut(s)	27
BseDI	CCNNGG	4 cut(s)	399, 1005, 1188, 1731
BseGI	GGATG	6 cut(s)	33, 72, 78, 111, 618, 1080
BseJI	GATNNNNATC	1 cut(s)	1212
BseLI	CCNNNNNNNGG	6 cut(s)	135, 182, 1263, 1656, 1799, 1814
BseMII	CTCAG	3 cut(s)	996, 1491, 1666
BseNI	ACTGG	3 cut(s)	1157, 1460, 1625
BseRI	GAGGAG	2 cut(s)	121, 521
BseSI	GKGCMC	1 cut(s)	1391
BseXI	GCAGC	2 cut(s)	529, 855
BsgI	GTGCAG	2 cut(s)	433, 545
Bsh1236I	CGCG	1 cut(s)	1785
Bsh1285I	CGRYCG	1 cut(s)	1378
BshFI	GGCC	6 cut(s)	162, 383, 398, 404, 420, 1010
BshNI	GGYRCC	5 cut(s)	429, 968, 1023, 1386, 1867
BshVI	ATCGAT	1 cut(s)	27
BsiEI	CGRYCG	1 cut(s)	1378
BsiHKAI	GWGCWC	2 cut(s)	1059, 1464
BsiHKCI	CYCGRG	1 cut(s)	923
BsiSI	CCGG	2 cut(s)	1374, 1731
BslI	CCNNNNNNNGG	6 cut(s)	135, 182, 1263, 1656, 1799, 1814
BsmAI	GTCTC	5 cut(s)	111, 298, 1049, 1675, 1831
BsmBI	CGTCTC	2 cut(s)	1049, 1831
BsmI	GAATGC	2 cut(s)	1479, 1599
BsnI	GGCC	6 cut(s)	162, 383, 398, 404, 420, 1010
Bso31I	GGTCTC	2 cut(s)	111, 298
BsoBI	CYCGRG	1 cut(s)	923
Bsp1286I	GDGCHC	3 cut(s)	1059, 1391, 1464
Bsp1407I	TGTACA	1 cut(s)	1563
Bsp143I	GATC	7 cut(s)	28, 646, 730, 805, 1068, 1078, 1267
Bsp19I	CCATGG	2 cut(s)	399, 1188
BspACI	CCGC	6 cut(s)	115, 352, 494, 545, 965, 1558
BspANI	GGCC	6 cut(s)	162, 383, 398, 404, 420, 1010
BspCNI	CTCAG	3 cut(s)	995, 1492, 1665
BspDI	ATCGAT	1 cut(s)	27
BspFNI	CGCG	1 cut(s)	1785
BspHI	TCATGA	1 cut(s)	808
BspLI	GGNNCC	8 cut(s)	431, 970, 1004, 1025, 1269, 1372, 1388, 1869
BspMI	ACCTGC	1 cut(s)	1562
BspOI	GCTAGC	1 cut(s)	351
BspPI	GGATC	4 cut(s)	813, 1086, 1262, 1275
BspQI	GCTCTTC	1 cut(s)	1047
BspT107I	GGYRCC	5 cut(s)	429, 968, 1023, 1386, 1867
BspTI	CTTAAG	1 cut(s)	1144
BspTNI	GGTCTC	2 cut(s)	111, 298
BsrGI	TGTACA	1 cut(s)	1563
BsrI	ACTGG	3 cut(s)	1157, 1460, 1625
BssECI	CCNNGG	4 cut(s)	399, 1005, 1188, 1731
BssMI	GATC	7 cut(s)	28, 646, 730, 805, 1068, 1078, 1267
BssNI	GRCGYC	1 cut(s)	430
BssT1I	CCWWGG	2 cut(s)	399, 1188
Bst2UI	CCWGG	3 cut(s)	159, 803, 1007
Bst4CI	ACNGT	5 cut(s)	218, 775, 1204, 1417, 1867
Bst6I	CTCTTC	3 cut(s)	752, 1047, 1375
BstACI	GRCGYC	1 cut(s)	430
BstAFI	CTTAAG	1 cut(s)	1144
BstAUI	TGTACA	1 cut(s)	1563
BstBAI	YACGTR	3 cut(s)	606, 1340, 1774
BstC8I	GCNNGC	4 cut(s)	349, 452, 553, 1542
BstDEI	CTNAG	6 cut(s)	497, 719, 982, 1500, 1652, 1872
BstDSI	CCRYGG	2 cut(s)	399, 1188
BstENI	CCTNNNNNAGG	2 cut(s)	133, 1797
BstF5I	GGATG	6 cut(s)	33, 72, 78, 111, 618, 1080
BstFNI	CGCG	1 cut(s)	1785
BstH2I	RGCGCY	1 cut(s)	433
BstHHI	GCGC	4 cut(s)	432, 753, 1785, 1805
BstKTI	GATC	7 cut(s)	31, 649, 733, 808, 1071, 1081, 1270
BstMAI	GTCTC	5 cut(s)	111, 298, 1049, 1675, 1831
BstMBI	GATC	7 cut(s)	28, 646, 730, 805, 1068, 1078, 1267
BstMCI	CGRYCG	1 cut(s)	1378
BstMWI	GCNNNNNNNGC	2 cut(s)	548, 1546
BstNI	CCWGG	3 cut(s)	159, 803, 1007
BstNSI	RCATGY	1 cut(s)	922
BstSCI	CCNGG	4 cut(s)	157, 801, 1005, 1730
BstSLI	GKGCMC	1 cut(s)	1391
BstUI	CGCG	1 cut(s)	1785
BstV1I	GCAGC	2 cut(s)	529, 855
BstX2I	RGATCY	1 cut(s)	1267
BstXI	CCANNNNNNTGG	2 cut(s)	1278, 1715
BstYI	RGATCY	1 cut(s)	1267
Bsu15I	ATCGAT	1 cut(s)	27
BsuRI	GGCC	6 cut(s)	162, 383, 398, 404, 420, 1010
BsuTUI	ATCGAT	1 cut(s)	27
BtgI	CCRYGG	2 cut(s)	399, 1188
BtrI	CACGTC	1 cut(s)	865
BtsCI	GGATG	6 cut(s)	33, 72, 78, 111, 618, 1080
BtsI	GCAGTG	2 cut(s)	170, 1444
BtsIMutI	CAGTG	2 cut(s)	170, 1444
BveI	ACCTGC	1 cut(s)	1562
Cac8I	GCNNGC	4 cut(s)	349, 452, 553, 1542
CaiI	CAGNNNCTG	1 cut(s)	532
CciI	TCATGA	1 cut(s)	808
CfoI	GCGC	4 cut(s)	432, 753, 1785, 1805
Cfr13I	GGNCC	6 cut(s)	161, 248, 419, 1002, 1254, 1371
ClaI	ATCGAT	1 cut(s)	27
CseI	GACGC	1 cut(s)	254
Csp6I	GTAC	5 cut(s)	376, 833, 1564, 1858, 1868
CspCI	CAANNNNNGTGG	4 cut(s)	270, 305, 976, 1011
CviQI	GTAC	5 cut(s)	376, 833, 1564, 1858, 1868
DdeI	CTNAG	6 cut(s)	497, 719, 982, 1500, 1652, 1872
DinI	GGCGCC	1 cut(s)	431
DpnI	GATC	7 cut(s)	30, 648, 732, 807, 1070, 1080, 1269
DpnII	GATC	7 cut(s)	28, 646, 730, 805, 1068, 1078, 1267
DriI	GACNNNNNGTC	1 cut(s)	1823
EaeI	YGGCCR	1 cut(s)	1008
Eam1104I	CTCTTC	3 cut(s)	752, 1047, 1375
Eam1105I	GACNNNNNGTC	1 cut(s)	1823
EarI	CTCTTC	3 cut(s)	752, 1047, 1375
EciI	GGCGGA	1 cut(s)	104
Ecl136II	GAGCTC	2 cut(s)	1057, 1462
Eco130I	CCWWGG	2 cut(s)	399, 1188
Eco24I	GRGCYC	2 cut(s)	1059, 1464
Eco31I	GGTCTC	2 cut(s)	111, 298
Eco32I	GATATC	1 cut(s)	1214
Eco47I	GGWCC	4 cut(s)	248, 1002, 1254, 1371
Eco53kI	GAGCTC	2 cut(s)	1057, 1462
Eco57I	CTGAAG	3 cut(s)	45, 554, 719
Eco72I	CACGTG	1 cut(s)	1774
Eco88I	CYCGRG	1 cut(s)	923
EcoICRI	GAGCTC	2 cut(s)	1057, 1462
EcoNI	CCTNNNNNAGG	2 cut(s)	133, 1797
EcoRI	GAATTC	1 cut(s)	1298
EcoRII	CCWGG	3 cut(s)	157, 801, 1005
EcoRV	GATATC	1 cut(s)	1214
EcoT14I	CCWWGG	2 cut(s)	399, 1188
EcoT38I	GRGCYC	2 cut(s)	1059, 1464
EgeI	GGCGCC	1 cut(s)	431
EheI	GGCGCC	1 cut(s)	431
ErhI	CCWWGG	2 cut(s)	399, 1188
Esp3I	CGTCTC	2 cut(s)	1049, 1831
FalI	AAGNNNNNCTT	2 cut(s)	1127, 1159
FauI	CCCGC	1 cut(s)	958
FauNDI	CATATG	1 cut(s)	195
FblI	GTMKAC	3 cut(s)	777, 861, 915
Fnu4HI	GCNGC	4 cut(s)	352, 543, 869, 1558
FokI	GGATG	6 cut(s)	20, 59, 65, 98, 625, 1087
FriOI	GRGCYC	2 cut(s)	1059, 1464
Fsp4HI	GCNGC	4 cut(s)	352, 543, 869, 1558
FspBI	CTAG	6 cut(s)	200, 233, 269, 348, 1172, 1467
GlaI	GCGC	4 cut(s)	431, 752, 1784, 1804
GluI	GCNGC	4 cut(s)	352, 543, 869, 1558
GsuI	CTGGAG	1 cut(s)	1043
HaeII	RGCGCY	1 cut(s)	433
HaeIII	GGCC	6 cut(s)	162, 383, 398, 404, 420, 1010
HapII	CCGG	2 cut(s)	1374, 1731
HgaI	GACGC	1 cut(s)	254
HhaI	GCGC	4 cut(s)	432, 753, 1785, 1805
Hin1I	GRCGYC	1 cut(s)	430
Hin6I	GCGC	4 cut(s)	430, 751, 1783, 1803
HinP1I	GCGC	4 cut(s)	430, 751, 1783, 1803
HincII	GTYRAC	3 cut(s)	778, 1333, 1410
HindII	GTYRAC	3 cut(s)	778, 1333, 1410
HinfI	GANTC	3 cut(s)	55, 600, 1236
HpaI	GTTAAC	1 cut(s)	1410
HpaII	CCGG	2 cut(s)	1374, 1731
HphI	GGTGA	3 cut(s)	596, 1749, 1750
Hpy166II	GTNNAC	7 cut(s)	725, 778, 862, 916, 1333, 1343, 1410
Hpy188I	TCNGA	5 cut(s)	64, 373, 441, 1285, 1655
Hpy188III	TCNNGA	9 cut(s)	37, 679, 809, 822, 1000, 1018, 1060, 1072, 1233
Hpy8I	GTNNAC	7 cut(s)	725, 778, 862, 916, 1333, 1343, 1410
HpyAV	CCTTC	5 cut(s)	69, 388, 495, 529, 571
HpyCH4III	ACNGT	5 cut(s)	218, 775, 1204, 1417, 1867
HpyCH4IV	ACGT	8 cut(s)	605, 864, 1042, 1329, 1339, 1773, 1812, 1824
HpyCH4V	TGCA	4 cut(s)	450, 526, 1399, 1444
HpyF10VI	GCNNNNNNNGC	2 cut(s)	548, 1546
HpyF3I	CTNAG	6 cut(s)	497, 719, 982, 1500, 1652, 1872
HpySE526I	ACGT	8 cut(s)	605, 864, 1042, 1329, 1339, 1773, 1812, 1824
Hsp92I	GRCGYC	1 cut(s)	430
HspAI	GCGC	4 cut(s)	430, 751, 1783, 1803
KasI	GGCGCC	1 cut(s)	429
KpnI	GGTACC	1 cut(s)	1871
KspAI	GTTAAC	1 cut(s)	1410
Kzo9I	GATC	7 cut(s)	28, 646, 730, 805, 1068, 1078, 1267
LguI	GCTCTTC	1 cut(s)	1047
LmnI	GCTCC	5 cut(s)	496, 686, 1062, 1459, 1467
Lsp1109I	GCAGC	2 cut(s)	529, 855
LweI	GCATC	1 cut(s)	1636
MaeI	CTAG	6 cut(s)	200, 233, 269, 348, 1172, 1467
MaeII	ACGT	8 cut(s)	605, 864, 1042, 1329, 1339, 1773, 1812, 1824
MaeIII	GTNAC	2 cut(s)	1149, 1769
MalI	GATC	7 cut(s)	30, 648, 732, 807, 1070, 1080, 1269
MboI	GATC	7 cut(s)	28, 646, 730, 805, 1068, 1078, 1267
MboII	GAAGA	8 cut(s)	182, 725, 769, 1064, 1103, 1307, 1392, 1686
MflI	RGATCY	1 cut(s)	1267
MhlI	GDGCHC	3 cut(s)	1059, 1391, 1464
MluCI	AATT	6 cut(s)	41, 1298, 1400, 1505, 1696, 1753
Mly113I	GGCGCC	1 cut(s)	430
MlyI	GAGTC	2 cut(s)	64, 1245
MseI	TTAA	4 cut(s)	407, 893, 1145, 1409
MslI	CAYNNNNRTG	2 cut(s)	100, 1713
MspCI	CTTAAG	1 cut(s)	1144
MspI	CCGG	2 cut(s)	1374, 1731
MspR9I	CCNGG	4 cut(s)	159, 803, 1007, 1732
Mva1269I	GAATGC	2 cut(s)	1479, 1599
MvaI	CCWGG	3 cut(s)	159, 803, 1007
MvnI	CGCG	1 cut(s)	1785
MwoI	GCNNNNNNNGC	2 cut(s)	548, 1546
NarI	GGCGCC	1 cut(s)	430
NciI	CCSGG	1 cut(s)	1732
NcoI	CCATGG	2 cut(s)	399, 1188
NdeI	CATATG	1 cut(s)	195
NdeII	GATC	7 cut(s)	28, 646, 730, 805, 1068, 1078, 1267
NheI	GCTAGC	1 cut(s)	347
NlaIV	GGNNCC	8 cut(s)	431, 970, 1004, 1025, 1269, 1372, 1388, 1869
NmuCI	GTSAC	1 cut(s)	1769
NspI	RCATGY	1 cut(s)	922
OliI	CACNNNNGTG	1 cut(s)	1713
PaeR7I	CTCGAG	1 cut(s)	923
PagI	TCATGA	1 cut(s)	808
PciSI	GCTCTTC	1 cut(s)	1047
PctI	GAATGC	2 cut(s)	1479, 1599
PfeI	GAWTC	1 cut(s)	600
PflMI	CCANNNNNTGG	1 cut(s)	182
PfoI	TCCNGGA	1 cut(s)	801
PkrI	GCNGC	4 cut(s)	353, 544, 870, 1559
PleI	GAGTC	2 cut(s)	63, 1244
PluTI	GGCGCC	1 cut(s)	433
PmaCI	CACGTG	1 cut(s)	1774
PmlI	CACGTG	1 cut(s)	1774
PpsI	GAGTC	2 cut(s)	63, 1244
Ppu21I	YACGTR	3 cut(s)	606, 1340, 1774
PsiI	TTATAA	1 cut(s)	1695
Psp124BI	GAGCTC	2 cut(s)	1059, 1464
Psp1406I	AACGTT	1 cut(s)	1329
Psp6I	CCWGG	3 cut(s)	157, 801, 1005
PspCI	CACGTG	1 cut(s)	1774
PspGI	CCWGG	3 cut(s)	157, 801, 1005
PspN4I	GGNNCC	8 cut(s)	431, 970, 1004, 1025, 1269, 1372, 1388, 1869
PspPI	GGNCC	6 cut(s)	161, 248, 419, 1002, 1254, 1371
PspXI	VCTCGAGB	1 cut(s)	923
PstNI	CAGNNNCTG	1 cut(s)	532
PsuI	RGATCY	1 cut(s)	1267
RsaI	GTAC	5 cut(s)	377, 834, 1565, 1859, 1869
RsaNI	GTAC	5 cut(s)	376, 833, 1564, 1858, 1868
RseI	CAYNNNNRTG	2 cut(s)	100, 1713
SacI	GAGCTC	2 cut(s)	1059, 1464
SalI	GTCGAC	1 cut(s)	776
SapI	GCTCTTC	1 cut(s)	1047
SaqAI	TTAA	4 cut(s)	407, 893, 1145, 1409
SatI	GCNGC	4 cut(s)	352, 543, 869, 1558
Sau3AI	GATC	7 cut(s)	28, 646, 730, 805, 1068, 1078, 1267
Sau96I	GGNCC	6 cut(s)	161, 248, 419, 1002, 1254, 1371
SchI	GAGTC	2 cut(s)	64, 1245
ScrFI	CCNGG	4 cut(s)	159, 803, 1007, 1732
SduI	GDGCHC	3 cut(s)	1059, 1391, 1464
SfaNI	GCATC	1 cut(s)	1636
SfoI	GGCGCC	1 cut(s)	431
Sfr274I	CTCGAG	1 cut(s)	923
SinI	GGWCC	4 cut(s)	248, 1002, 1254, 1371
SlaI	CTCGAG	1 cut(s)	923
SmiMI	CAYNNNNRTG	2 cut(s)	100, 1713
SmlI	CTYRAG	5 cut(s)	133, 923, 1018, 1120, 1144
SmoI	CTYRAG	5 cut(s)	133, 923, 1018, 1120, 1144
SpeI	ACTAGT	1 cut(s)	1171
Sse9I	AATT	6 cut(s)	41, 1298, 1400, 1505, 1696, 1753
SsiI	CCGC	6 cut(s)	115, 352, 494, 545, 965, 1558
SspDI	GGCGCC	1 cut(s)	429
SspI	AATATT	1 cut(s)	1249
SspMI	CTAG	6 cut(s)	200, 233, 269, 348, 1172, 1467
SstI	GAGCTC	2 cut(s)	1059, 1464
StyD4I	CCNGG	4 cut(s)	157, 801, 1005, 1730
StyI	CCWWGG	2 cut(s)	399, 1188
TaaI	ACNGT	5 cut(s)	218, 775, 1204, 1417, 1867
TaiI	ACGT	8 cut(s)	608, 867, 1045, 1332, 1342, 1776, 1815, 1827
TasI	AATT	6 cut(s)	41, 1298, 1400, 1505, 1696, 1753
TatI	WGTACW	2 cut(s)	375, 1563
TauI	GCSGC	2 cut(s)	354, 1560
TfiI	GAWTC	1 cut(s)	600
Tru1I	TTAA	4 cut(s)	407, 893, 1145, 1409
Tru9I	TTAA	4 cut(s)	407, 893, 1145, 1409
TscAI	CASTG	2 cut(s)	177, 1451
TseFI	GTSAC	1 cut(s)	1769
TseI	GCWGC	2 cut(s)	542, 868
Tsp45I	GTSAC	1 cut(s)	1769
TspDTI	ATGAA	7 cut(s)	182, 265, 770, 825, 864, 1052, 1334
TspGWI	ACGGA	6 cut(s)	1600, 1605, 1640, 1645, 1833, 1834
TspRI	CASTG	2 cut(s)	177, 1451
Van91I	CCANNNNNTGG	1 cut(s)	182
Vha464I	CTTAAG	1 cut(s)	1144
VpaK11BI	GGWCC	4 cut(s)	248, 1002, 1254, 1371
XagI	CCTNNNNNAGG	2 cut(s)	133, 1797
XapI	RAATTY	3 cut(s)	1298, 1505, 1753
XceI	RCATGY	1 cut(s)	922
XhoI	CTCGAG	1 cut(s)	923
XmiI	GTMKAC	3 cut(s)	777, 861, 915
XspI	CTAG	6 cut(s)	200, 233, 269, 348, 1172, 1467

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.