Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0000282

Physical Location & Seq

Genomic Coordinates Forward (+)

30636094 .. 30636543

Gene Structure 450 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1225400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 450 bp

ATGGAGGACATCACGGTTGATGGATATCATATACCCAAGAAATCCACCATCTTTGTGAACATCTGGACGATTGGGCGAGATCCAAGTGTATGGTCAGATAATGTGGAAGAGTTCTACTCTGAAAGGTTTAATGACAGTGACGTTGACCTTAAGGGGCATGACTTCCAGCTTTTACCATTTGGGTCCGGAAGAAGGGGGTGCCCTGGCATGCAACTAGGGTTAACCACGGTTCGTCTAGTTCTGGTAAATTTGGTCCACTGCTTCAATTTTGATCTCCCAAGTGGCCTCCTTCCTAACGAGTTGGACATGACTGAGACTTTTAGGCAGTCGCTGTCAAAAACCAAACACTTGCTTGTCGTGCCCACGTATCACCTACACGATTTAACATTCATAGAGATTAGGTTTTGCATATGGTGTGTATATACCATTGTTCTAAAAATTCTCGCCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004497 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0008150 GO:0008152 GO:0009058 GO:0012505 GO:0016020 GO:0016491 GO:0016705 GO:0016709 GO:0019748 GO:0031090 GO:0031984 GO:0042175 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0044550 GO:0055114 GO:0098827

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

p450

Protein Analysis

150

Amino Acids

17.16

Weight (kDa)

5.59

Isoelectric Point (pI)

42.48

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
p450	PF00067	2 - 102	1e-26	Cytochrome P450

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready

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Orthologous Genes (Group: OG0000282)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_5g12350 FvH4_5g12380 FvH4_5g12392 FvH4_5g12400 FvH4_5g12430
malus_domestica	MD06G1213300.v1.1 MD06G1213500.v1.1 MD06G1213700.v1.1 MD06G1213800.v1.1 MD06G1213900.v1.1 MD06G1214000.v1.1 MD06G1214400.v1.1 MD14G1224300.v1.1 MD14G1224400.v1.1 MD14G1224600.v1.1 MD14G1224700.v1.1 MD14G1224800.v1.1 MD14G1224900.v1.1 MD14G1225100.v1.1 MD14G1225400.v1.1
prunus_persica	Prupe.5G219700_v2.0.a1 Prupe.5G219800_v2.0.a1 Prupe.5G219900_v2.0.a1 Prupe.8G078500_v2.0.a1
pyrus_communis	pycom06g19100 pycom14g18590 pycom14g18600 pycom14g18610 pycom14g18660
rosa_chinensis	RchiOBHm_Chr0c12g0499591 RchiOBHm_Chr2g0140591 RchiOBHm_Chr2g0140631 RchiOBHm_Chr2g0140641 RchiOBHm_Chr2g0140651 RchiOBHm_Chr4g0417991 RchiOBHm_Chr4g0418001 RchiOBHm_Chr4g0418101 RchiOBHm_Chr4g0418111 RchiOBHm_Chr4g0418121 RchiOBHm_Chr6g0280191 RchiOBHm_Chr6g0280241 RchiOBHm_Chr6g0280251 RchiOBHm_Chr7g0185031 RchiOBHm_Chr7g0185051 RchiOBHm_Chr7g0185081 RchiOBHm_Chr7g0185091 RchiOBHm_Chr7g0185111 RchiOBHm_Chr7g0185131 RchiOBHm_Chr7g0185151 RchiOBHm_Chr7g0185161 RchiOBHm_Chr7g0185191 RchiOBHm_Chr7g0222431
rosa_laevigata	RLG00000002061 RLG00000004934 RLG00000004935 RLG00000004937 RLG00000013071 RLG00000013077 RLG00000019621 RLG00000019881 RLG00000019884 RLG00000019887 RLG00000019888 RLG00000028839
rosa_multiflora	Rmu_co8014370.1_g000001 Rmu_co8334683.1_g000001 Rmu_co8440383.1_g000001 Rmu_sc0002826.1_g000008 Rmu_sc0002826.1_g000009 Rmu_sc0002826.1_g000030 Rmu_sc0004483.1_g000005 Rmu_sc0004638.1_g000003 Rmu_sc0004638.1_g000004 Rmu_sc0005756.1_g000003 Rmu_sc0006493.1_g000023 Rmu_sc0006493.1_g000024 Rmu_sc0007137.1_g000017 Rmu_sc0008496.1_g000022 Rmu_sc0008769.1_g000003 Rmu_sc0013612.1_g000026 Rmu_sc0013864.1_g000001 Rmu_sc0013864.1_g000007 Rmu_sc0013864.1_g000008 Rmu_sc0030128.1_g000001 Rmu_sc0041660.1_g000001 Rmu_ssc0000289.1_g000001
rosa_roxburghii	Rroxscaffold_2G00104640 Rroxscaffold_3G00253970 Rroxscaffold_3G00269170 Rroxscaffold_3G00269200 Rroxscaffold_3G00269210 Rroxscaffold_3G00269220 Rroxscaffold_3G00269230 Rroxscaffold_7G00188320
rosa_rugosa	Rorug01G0052300 Rorug05G0212000 Rorug06G0130700 Rorug06G0131100 Rorug06G0466200 Rorug06G0466300 Rorug06G0466400 Rorug06G0466500 Rorug06G0466700 Rorug07G0208500 Rorug07G0294600
rosa_samantha	Rh4AG137600 Rh6DG237300 Rh6DG237800 Rh7AG076200 Rh7AG076300 Rh7AG076500 Rh7AG076600 Rh7AG350700 Rh7BG070800 Rh7BG070900 Rh7BG071000 Rh7BG071100
rosa_wichuraiana	Rw6G020990 Rw6G021010 Rw7G005900 Rw7G005910 Rw7G005920 Rw7G005940 Rw7G005950 Rw7G005970 Rw7G029600

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	198
AccIII	TCCGGA	1 cut(s)	185
AclWI	GGATC	1 cut(s)	74
AcsI	RAATTY	2 cut(s)	247, 438
AfiI	CCNNNNNNNGG	1 cut(s)	192
AflII	CTTAAG	1 cut(s)	149
AgsI	TTSAA	1 cut(s)	265
AjnI	CCWGG	1 cut(s)	202
AluBI	AGCT	1 cut(s)	169
AluI	AGCT	1 cut(s)	169
Alw26I	GTCTC	1 cut(s)	308
AlwI	GGATC	1 cut(s)	74
AlwNI	CAGNNNCTG	1 cut(s)	331
Aor13HI	TCCGGA	1 cut(s)	185
AoxI	GGCC	1 cut(s)	283
ApoI	RAATTY	2 cut(s)	247, 438
AspS9I	GGNCC	2 cut(s)	183, 253
AsuHPI	GGTGA	1 cut(s)	362
AvaII	GGWCC	2 cut(s)	183, 253
BaeGI	GKGCMC	2 cut(s)	203, 363
BanI	GGYRCC	1 cut(s)	198
BccI	CCATC	2 cut(s)	14, 56
BciT130I	CCWGG	1 cut(s)	204
BcoDI	GTCTC	1 cut(s)	308
BfaI	CTAG	3 cut(s)	215, 236, 448
BfrI	CTTAAG	1 cut(s)	149
Bme1390I	CCNGG	1 cut(s)	204
Bme18I	GGWCC	2 cut(s)	183, 253
BmgT120I	GGNCC	2 cut(s)	183, 253
BmiI	GGNNCC	2 cut(s)	184, 200
BmrFI	CCNGG	1 cut(s)	204
BplI	GAGNNNNNCTC	2 cut(s)	101, 133
BsaAI	YACGTR	1 cut(s)	366
BsaBI	GATNNNNATC	1 cut(s)	24
BsaJI	CCNNGG	2 cut(s)	202, 225
BsaWI	WCCGGW	1 cut(s)	185
Bsc4I	CCNNNNNNNGG	1 cut(s)	192
Bse8I	GATNNNNATC	1 cut(s)	24
BseAI	TCCGGA	1 cut(s)	185
BseBI	CCWGG	1 cut(s)	204
BseDI	CCNNGG	2 cut(s)	202, 225
BseJI	GATNNNNATC	1 cut(s)	24
BseLI	CCNNNNNNNGG	1 cut(s)	192
BseMII	CTCAG	1 cut(s)	303
BseSI	GKGCMC	2 cut(s)	203, 363
BshFI	GGCC	1 cut(s)	285
BshNI	GGYRCC	1 cut(s)	198
BsiSI	CCGG	1 cut(s)	186
BslI	CCNNNNNNNGG	1 cut(s)	192
BsmAI	GTCTC	1 cut(s)	308
BsnI	GGCC	1 cut(s)	285
Bsp1286I	GDGCHC	2 cut(s)	203, 363
Bsp13I	TCCGGA	1 cut(s)	185
Bsp143I	GATC	2 cut(s)	79, 271
BspANI	GGCC	1 cut(s)	285
BspCNI	CTCAG	1 cut(s)	304
BspEI	TCCGGA	1 cut(s)	185
BspLI	GGNNCC	2 cut(s)	184, 200
BspPI	GGATC	1 cut(s)	74
BspT107I	GGYRCC	1 cut(s)	198
BspTI	CTTAAG	1 cut(s)	149
BssECI	CCNNGG	2 cut(s)	202, 225
BssMI	GATC	2 cut(s)	79, 271
Bst2UI	CCWGG	1 cut(s)	204
Bst4CI	ACNGT	3 cut(s)	16, 137, 229
Bst6I	CTCTTC	1 cut(s)	102
BstAFI	CTTAAG	1 cut(s)	149
BstBAI	YACGTR	1 cut(s)	366
BstC8I	GCNNGC	1 cut(s)	209
BstDEI	CTNAG	1 cut(s)	312
BstDSI	CCRYGG	1 cut(s)	225
BstKTI	GATC	2 cut(s)	82, 274
BstMAI	GTCTC	1 cut(s)	308
BstMBI	GATC	2 cut(s)	79, 271
BstMWI	GCNNNNNNNGC	1 cut(s)	358
BstNI	CCWGG	1 cut(s)	204
BstNSI	RCATGY	1 cut(s)	211
BstSCI	CCNGG	1 cut(s)	202
BstSLI	GKGCMC	2 cut(s)	203, 363
BstX2I	RGATCY	1 cut(s)	79
BstXI	CCANNNNNNTGG	1 cut(s)	90
BstYI	RGATCY	1 cut(s)	79
BsuRI	GGCC	1 cut(s)	285
BtgI	CCRYGG	1 cut(s)	225
BtsI	GCAGTG	1 cut(s)	256
BtsIMutI	CAGTG	2 cut(s)	142, 256
Cac8I	GCNNGC	1 cut(s)	209
CaiI	CAGNNNCTG	1 cut(s)	331
Cfr13I	GGNCC	2 cut(s)	183, 253
CspCI	CAANNNNNGTGG	2 cut(s)	34, 69
CviAII	CATG	3 cut(s)	158, 208, 307
CviJI	RGCY	2 cut(s)	169, 285
CviKI_1	RGCY	2 cut(s)	169, 285
DdeI	CTNAG	1 cut(s)	312
DpnI	GATC	2 cut(s)	81, 273
DpnII	GATC	2 cut(s)	79, 271
Eam1104I	CTCTTC	1 cut(s)	102
EarI	CTCTTC	1 cut(s)	102
Eco32I	GATATC	1 cut(s)	26
Eco47I	GGWCC	2 cut(s)	183, 253
EcoRII	CCWGG	1 cut(s)	202
EcoRV	GATATC	1 cut(s)	26
FaeI	CATG	3 cut(s)	161, 211, 310
FatI	CATG	3 cut(s)	157, 207, 306
FauNDI	CATATG	1 cut(s)	410
FspBI	CTAG	3 cut(s)	215, 236, 448
HaeIII	GGCC	1 cut(s)	285
HapII	CCGG	1 cut(s)	186
Hin1II	CATG	3 cut(s)	161, 211, 310
HincII	GTYRAC	2 cut(s)	145, 222
HindII	GTYRAC	2 cut(s)	145, 222
HpaI	GTTAAC	1 cut(s)	222
HpaII	CCGG	1 cut(s)	186
HphI	GGTGA	1 cut(s)	362
Hpy166II	GTNNAC	4 cut(s)	58, 145, 222, 256
Hpy188I	TCNGA	2 cut(s)	97, 121
Hpy188III	TCNNGA	2 cut(s)	64, 186
Hpy8I	GTNNAC	4 cut(s)	58, 145, 222, 256
HpyAV	CCTTC	2 cut(s)	186, 299
HpyCH4III	ACNGT	3 cut(s)	16, 137, 229
HpyCH4IV	ACGT	2 cut(s)	141, 365
HpyCH4V	TGCA	2 cut(s)	211, 408
HpyF10VI	GCNNNNNNNGC	1 cut(s)	358
HpyF3I	CTNAG	1 cut(s)	312
HpySE526I	ACGT	2 cut(s)	141, 365
Hsp92II	CATG	3 cut(s)	161, 211, 310
Kpn2I	TCCGGA	1 cut(s)	185
KspAI	GTTAAC	1 cut(s)	222
Kzo9I	GATC	2 cut(s)	79, 271
LpnPI	CCDG	6 cut(s)	49, 179, 189, 199, 216, 227
MaeI	CTAG	3 cut(s)	215, 236, 448
MaeII	ACGT	2 cut(s)	141, 365
MaeIII	GTNAC	1 cut(s)	137
MalI	GATC	2 cut(s)	81, 273
MboI	GATC	2 cut(s)	79, 271
MboII	GAAGA	2 cut(s)	119, 201
MflI	RGATCY	1 cut(s)	79
MhlI	GDGCHC	2 cut(s)	203, 363
MluCI	AATT	3 cut(s)	247, 265, 438
MmeI	TCCRAC	1 cut(s)	282
MnlI	CCTC	1 cut(s)	296
MroI	TCCGGA	1 cut(s)	185
MseI	TTAA	4 cut(s)	129, 150, 221, 383
MslI	CAYNNNNRTG	1 cut(s)	53
MspCI	CTTAAG	1 cut(s)	149
MspI	CCGG	1 cut(s)	186
MspR9I	CCNGG	1 cut(s)	204
MvaI	CCWGG	1 cut(s)	204
MwoI	GCNNNNNNNGC	1 cut(s)	358
NdeI	CATATG	1 cut(s)	410
NdeII	GATC	2 cut(s)	79, 271
NlaIII	CATG	3 cut(s)	161, 211, 310
NlaIV	GGNNCC	2 cut(s)	184, 200
NmuCI	GTSAC	1 cut(s)	137
NspI	RCATGY	1 cut(s)	211
PaeI	GCATGC	1 cut(s)	211
Ppu21I	YACGTR	1 cut(s)	366
Psp6I	CCWGG	1 cut(s)	202
PspGI	CCWGG	1 cut(s)	202
PspN4I	GGNNCC	2 cut(s)	184, 200
PspPI	GGNCC	2 cut(s)	183, 253
PstNI	CAGNNNCTG	1 cut(s)	331
PsuI	RGATCY	1 cut(s)	79
RseI	CAYNNNNRTG	1 cut(s)	53
SaqAI	TTAA	4 cut(s)	129, 150, 221, 383
Sau3AI	GATC	2 cut(s)	79, 271
Sau96I	GGNCC	2 cut(s)	183, 253
ScrFI	CCNGG	1 cut(s)	204
SduI	GDGCHC	2 cut(s)	203, 363
SetI	ASST	7 cut(s)	128, 144, 150, 171, 368, 375, 404
SinI	GGWCC	2 cut(s)	183, 253
SmiMI	CAYNNNNRTG	1 cut(s)	53
SmlI	CTYRAG	1 cut(s)	149
SmoI	CTYRAG	1 cut(s)	149
SphI	GCATGC	1 cut(s)	211
Sse9I	AATT	3 cut(s)	247, 265, 438
SspMI	CTAG	3 cut(s)	215, 236, 448
StyD4I	CCNGG	1 cut(s)	202
TaaI	ACNGT	3 cut(s)	16, 137, 229
TaiI	ACGT	2 cut(s)	144, 368
TasI	AATT	3 cut(s)	247, 265, 438
Tru1I	TTAA	4 cut(s)	129, 150, 221, 383
Tru9I	TTAA	4 cut(s)	129, 150, 221, 383
TscAI	CASTG	2 cut(s)	142, 263
TseFI	GTSAC	1 cut(s)	137
Tsp45I	GTSAC	1 cut(s)	137
TspDTI	ATGAA	1 cut(s)	379
TspRI	CASTG	2 cut(s)	142, 263
Vha464I	CTTAAG	1 cut(s)	149
VpaK11BI	GGWCC	2 cut(s)	183, 253
XapI	RAATTY	2 cut(s)	247, 438
XceI	RCATGY	1 cut(s)	211
XspI	CTAG	3 cut(s)	215, 236, 448

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.