Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000003

Orthogroup ID

OG0000282

Physical Location & Seq

Genomic Coordinates Reverse (-)

62086878 .. 62091954

Gene Structure 5077 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_3G00269170.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1584 bp

ATGAGTCCTTCAACAATAATAGCCATCCTCCTGGTTCTCCTCACATGCCTTTGGTCACTCATCTCTGCTTCCTCCAAATCAAAACACAAAAAACTACCACCCGGCCCTCGGTCACTGCCAATAATCGGAAATCTCCACATGCTAGGCAACCTCCCCCATCGAAGCCTCCAACACCTGGCCAAAAAATATGGACACATCATGTCCATCCGTCTAGGCAGTGTTCCGACCATAGTAGTCTCCTCCCCCAAAGCCGCAGAGCTATTCCTCAAAACTCATGACACTAATTTCGCCAGCCGGCCCAAAATCCAAGCCTCGGAGTACATATCCTACGGCACAAAGGGCATGGCCTTTTCCGAATGTGGTCCCTATTGGCGCCATGTAAGGAAGCTCTGCACGCTTCAGCTTTTCTGTCCAGCAAAAATAGAGGCTTTCGCGCCGCTGAGAAAGGATGAGCTGGGAGTGCTGCTGAGAAAGCTCAAGAAAGCTGCAGAGGAAGGTGGTGTTGTGGATGTTAATGAGGATATTGGTGTGATGAATGAGGACATTACGTACAGGATGGTGTTGGGTTGTAAAAGGGATGATCAGTTTGATTTTACGCCGAAAGTCCTTGCAACAAAGGCAAGAGAACCCGCCGGCCGAGACGGTTCCTTCCTCGGCCCACGCTCACTTGAAAGAAGTCAAATCAAGCGCAAATCTTATCAAAACCTCGCTTGGCCAATTGGCCGCGAGTTGGCACGCCTGCGTATCAAGAAGGACAATTGGACTTCAAATCCTCGGCCCTCATTCGGCCGAGACGATTTTGAGGCAAACGATTTGAAAGCCATTGTTGAGGAAACGTTTTTCTTGGCCGGGGCTTTCAATATATCTGATTTTGTTCCTTCCCTTGCTGCACTTGATATTCAGGGGTTGACTAAGCGAATGAAGAAAGTTAGCAAGACGATCGATCTGCTCTTGGAGAAGATAATTGACGACCACGAACAAGTTGCTAAAACTACGAGTGGGAAACAAGGCAAGCATGAGGACTTTCCTTTCCGAGCTCTTAAGGCATCCAAGGGTCATGAAGAACTCCAAAAAGAGCTCCAAACTGTGGTTGGCATGGACCGAATGGTGGAAGAGACTGATTTGCCGAAGCTGGAATACTTGAGTATGGTGGTCAAGGAGAGCTTGAGACTACACCCGGTGGCACCATTACTAGTCCCACATGAATCCATCGAGGACATTACAATTGATGGATACGACATCCCCAAGAAGTCGCGAATCATAGTGAATTTCTGGAGCATTGGAAGAGATCCTAATGTTTGGTCAGAAAATGTTGAGGAATTTTATCCTGAAAGGTTCATGAACAGCAATATAGACCTCCGGGGACATGACTTTCAGCTCATCCCATTTGGGTCAGGCCGAAGAGGTTGTCCGGGTCTGCAATTAGGGCTAACCACAGTTCGGCTAGTTCTGGCGCAGCTGGTGCACTGCTTTAACTGGGAGCTCCCCGCTGTTGAGGTAGACTTGGACATGTCTGAGAAATTCGGTTTATCAATGTCAAAGGCCACACACTTGCTTGCCAAGCCGACCTACCGTCTTCTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004497 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0008150 GO:0008152 GO:0009058 GO:0012505 GO:0016020 GO:0016491 GO:0016705 GO:0016709 GO:0019748 GO:0031090 GO:0031984 GO:0042175 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0044550 GO:0055114 GO:0098827

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

p450

Protein Analysis

527

Amino Acids

59.38

Weight (kDa)

9.12

Isoelectric Point (pI)

44.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
p450	PF00067	33 - 193	1.2e-24	Cytochrome P450
p450	PF00067	354 - 514	6.7e-49	Cytochrome P450

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000282)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_5g12350 FvH4_5g12380 FvH4_5g12392 FvH4_5g12400 FvH4_5g12430
malus_domestica	MD06G1213300.v1.1 MD06G1213500.v1.1 MD06G1213700.v1.1 MD06G1213800.v1.1 MD06G1213900.v1.1 MD06G1214000.v1.1 MD06G1214400.v1.1 MD14G1224300.v1.1 MD14G1224400.v1.1 MD14G1224600.v1.1 MD14G1224700.v1.1 MD14G1224800.v1.1 MD14G1224900.v1.1 MD14G1225100.v1.1 MD14G1225400.v1.1
prunus_persica	Prupe.5G219700_v2.0.a1 Prupe.5G219800_v2.0.a1 Prupe.5G219900_v2.0.a1 Prupe.8G078500_v2.0.a1
pyrus_communis	pycom06g19100 pycom14g18590 pycom14g18600 pycom14g18610 pycom14g18660
rosa_chinensis	RchiOBHm_Chr0c12g0499591 RchiOBHm_Chr2g0140591 RchiOBHm_Chr2g0140631 RchiOBHm_Chr2g0140641 RchiOBHm_Chr2g0140651 RchiOBHm_Chr4g0417991 RchiOBHm_Chr4g0418001 RchiOBHm_Chr4g0418101 RchiOBHm_Chr4g0418111 RchiOBHm_Chr4g0418121 RchiOBHm_Chr6g0280191 RchiOBHm_Chr6g0280241 RchiOBHm_Chr6g0280251 RchiOBHm_Chr7g0185031 RchiOBHm_Chr7g0185051 RchiOBHm_Chr7g0185081 RchiOBHm_Chr7g0185091 RchiOBHm_Chr7g0185111 RchiOBHm_Chr7g0185131 RchiOBHm_Chr7g0185151 RchiOBHm_Chr7g0185161 RchiOBHm_Chr7g0185191 RchiOBHm_Chr7g0222431
rosa_laevigata	RLG00000002061 RLG00000004934 RLG00000004935 RLG00000004937 RLG00000013071 RLG00000013077 RLG00000019621 RLG00000019881 RLG00000019884 RLG00000019887 RLG00000019888 RLG00000028839
rosa_multiflora	Rmu_co8014370.1_g000001 Rmu_co8334683.1_g000001 Rmu_co8440383.1_g000001 Rmu_sc0002826.1_g000008 Rmu_sc0002826.1_g000009 Rmu_sc0002826.1_g000030 Rmu_sc0004483.1_g000005 Rmu_sc0004638.1_g000003 Rmu_sc0004638.1_g000004 Rmu_sc0005756.1_g000003 Rmu_sc0006493.1_g000023 Rmu_sc0006493.1_g000024 Rmu_sc0007137.1_g000017 Rmu_sc0008496.1_g000022 Rmu_sc0008769.1_g000003 Rmu_sc0013612.1_g000026 Rmu_sc0013864.1_g000001 Rmu_sc0013864.1_g000007 Rmu_sc0013864.1_g000008 Rmu_sc0030128.1_g000001 Rmu_sc0041660.1_g000001 Rmu_ssc0000289.1_g000001
rosa_roxburghii	Rroxscaffold_2G00104640 Rroxscaffold_3G00253970 Rroxscaffold_3G00269170 Rroxscaffold_3G00269200 Rroxscaffold_3G00269210 Rroxscaffold_3G00269220 Rroxscaffold_3G00269230 Rroxscaffold_7G00188320
rosa_rugosa	Rorug01G0052300 Rorug05G0212000 Rorug06G0130700 Rorug06G0131100 Rorug06G0466200 Rorug06G0466300 Rorug06G0466400 Rorug06G0466500 Rorug06G0466700 Rorug07G0208500 Rorug07G0294600
rosa_samantha	Rh4AG137600 Rh6DG237300 Rh6DG237800 Rh7AG076200 Rh7AG076300 Rh7AG076500 Rh7AG076600 Rh7AG350700 Rh7BG070800 Rh7BG070900 Rh7BG071000 Rh7BG071100
rosa_wichuraiana	Rw6G020990 Rw6G021010 Rw7G005900 Rw7G005910 Rw7G005920 Rw7G005940 Rw7G005950 Rw7G005970 Rw7G029600

Restriction Enzyme Sites

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Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	372, 1183
AccB7I	CCANNNNNTGG	2 cut(s)	175, 1087
AccI	GTMKAC	1 cut(s)	1500
AccII	CGCG	3 cut(s)	434, 726, 1255
AciI	CCGC	5 cut(s)	252, 437, 630, 724, 1488
AclI	AACGTT	1 cut(s)	836
AclWI	GGATC	1 cut(s)	1283
AcoI	YGGCCR	6 cut(s)	177, 634, 713, 721, 787, 846
AcsI	RAATTY	3 cut(s)	1267, 1319, 1520
AcuI	CTGAAG	1 cut(s)	383
AcyI	GRCGYC	1 cut(s)	373
AdeI	CACNNNGTG	1 cut(s)	1180
AfaI	GTAC	2 cut(s)	320, 551
AfiI	CCNNNNNNNGG	9 cut(s)	108, 125, 175, 313, 730, 785, 1087, 1108, 1440
AflII	CTTAAG	1 cut(s)	1040
AflIII	ACRYGT	1 cut(s)	1509
AgsI	TTSAA	5 cut(s)	12, 671, 768, 817, 859
AhdI	GACNNNNNGTC	1 cut(s)	1572
AhlI	ACTAGT	1 cut(s)	1192
AjnI	CCWGG	2 cut(s)	30, 174
Alw21I	GWGCWC	4 cut(s)	1039, 1080, 1467, 1485
Alw26I	GTCTC	5 cut(s)	241, 633, 786, 1109, 1162
Alw44I	GTGCAC	1 cut(s)	1463
AlwI	GGATC	1 cut(s)	1283
ApaLI	GTGCAC	1 cut(s)	1463
ApeKI	GCWGC	4 cut(s)	463, 485, 887, 1456
ApoI	RAATTY	3 cut(s)	1267, 1319, 1520
ArsI	GACNNNNNNTTYG	4 cut(s)	269, 301, 1393, 1425
AspLEI	GCGC	4 cut(s)	375, 436, 690, 1456
AspS9I	GGNCC	6 cut(s)	104, 297, 362, 656, 777, 1099
AsuC2I	CCSGG	5 cut(s)	102, 850, 1178, 1361, 1413
AvaII	GGWCC	2 cut(s)	362, 1099
BaeGI	GKGCMC	1 cut(s)	1467
BalI	TGGCCA	2 cut(s)	179, 715
BanI	GGYRCC	2 cut(s)	372, 1183
BanII	GRGCYC	3 cut(s)	1039, 1080, 1485
BarI	GAAGNNNNNNTAC	2 cut(s)	1121, 1153
BbsI	GAAGAC	1 cut(s)	1568
Bbv12I	GWGCWC	4 cut(s)	1039, 1080, 1467, 1485
BbvI	GCAGC	4 cut(s)	450, 472, 874, 1468
BccI	CCATC	6 cut(s)	32, 165, 212, 550, 1217, 1223
BceAI	ACGGC	1 cut(s)	346
BcgI	CGANNNNNNTGC	6 cut(s)	922, 928, 956, 962, 965, 999
BciT130I	CCWGG	2 cut(s)	32, 176
BciVI	GTATCC	1 cut(s)	1226
BclI	TGATCA	1 cut(s)	580
BcnI	CCSGG	5 cut(s)	102, 850, 1178, 1361, 1413
BcoDI	GTCTC	5 cut(s)	241, 633, 786, 1109, 1162
BcuI	ACTAGT	1 cut(s)	1192
BfaI	CTAG	4 cut(s)	143, 212, 1193, 1445
BfmI	CTRYAG	2 cut(s)	486, 1580
BfoI	RGCGCY	1 cut(s)	376
BfrI	CTTAAG	1 cut(s)	1040
BfuI	GTATCC	1 cut(s)	1226
BisI	GCNGC	7 cut(s)	252, 437, 464, 486, 724, 888, 1457
BlsI	GCNGC	7 cut(s)	253, 438, 465, 487, 725, 889, 1458
Bme1390I	CCNGG	7 cut(s)	32, 102, 176, 850, 1178, 1361, 1413
Bme18I	GGWCC	2 cut(s)	362, 1099
BmeRI	GACNNNNNGTC	1 cut(s)	1572
BmgT120I	GGNCC	6 cut(s)	104, 297, 362, 656, 777, 1099
BmiI	GGNNCC	4 cut(s)	364, 374, 646, 1185
BmrFI	CCNGG	7 cut(s)	32, 102, 176, 850, 1178, 1361, 1413
BmrI	ACTGGG	1 cut(s)	1486
BmsI	GCATC	1 cut(s)	1055
BmuI	ACTGGG	1 cut(s)	1486
BpiI	GAAGAC	1 cut(s)	1568
BpmI	CTGGAG	1 cut(s)	1294
BpuEI	CTTGAG	3 cut(s)	461, 1162, 1186
BpuMI	CCSGG	5 cut(s)	102, 850, 1178, 1361, 1413
Bsa29I	ATCGAT	1 cut(s)	942
BsaAI	YACGTR	1 cut(s)	549
BsaHI	GRCGYC	1 cut(s)	373
BsaJI	CCNNGG	7 cut(s)	107, 312, 652, 773, 849, 1050, 1360
Bsc4I	CCNNNNNNNGG	9 cut(s)	108, 125, 175, 313, 730, 785, 1087, 1108, 1440
Bse118I	RCCGGY	2 cut(s)	294, 632
Bse1I	ACTGG	1 cut(s)	1481
BseBI	CCWGG	2 cut(s)	32, 176
BseCI	ATCGAT	1 cut(s)	942
BseDI	CCNNGG	7 cut(s)	107, 312, 652, 773, 849, 1050, 1360
BseGI	GGATG	9 cut(s)	24, 204, 454, 514, 561, 583, 1046, 1239, 1380
BseLI	CCNNNNNNNGG	9 cut(s)	108, 125, 175, 313, 730, 785, 1087, 1108, 1440
BseMII	CTCAG	3 cut(s)	431, 458, 1506
BseNI	ACTGG	1 cut(s)	1481
BseRI	GAGGAG	2 cut(s)	29, 229
BseSI	GKGCMC	1 cut(s)	1467
BseX3I	CGGCCG	2 cut(s)	634, 787
BseXI	GCAGC	4 cut(s)	450, 472, 874, 1468
BseYI	CCCAGC	1 cut(s)	454
BsgI	GTGCAG	2 cut(s)	376, 873
Bsh1236I	CGCG	3 cut(s)	434, 726, 1255
Bsh1285I	CGRYCG	3 cut(s)	637, 790, 942
BshNI	GGYRCC	2 cut(s)	372, 1183
BshVI	ATCGAT	1 cut(s)	942
BsiEI	CGRYCG	3 cut(s)	637, 790, 942
BsiHKAI	GWGCWC	4 cut(s)	1039, 1080, 1467, 1485
BsiSI	CCGG	7 cut(s)	102, 295, 633, 849, 1178, 1360, 1412
BslFI	GGGAC	3 cut(s)	348, 1181, 1377
BslI	CCNNNNNNNGG	9 cut(s)	108, 125, 175, 313, 730, 785, 1087, 1108, 1440
BsmAI	GTCTC	5 cut(s)	241, 633, 786, 1109, 1162
BsmBI	CGTCTC	2 cut(s)	633, 786
BsmFI	GGGAC	3 cut(s)	348, 1181, 1377
Bsp1286I	GDGCHC	4 cut(s)	1039, 1080, 1467, 1485
Bsp143I	GATC	4 cut(s)	580, 939, 943, 1288
Bsp68I	TCGCGA	1 cut(s)	1255
BspACI	CCGC	5 cut(s)	252, 437, 630, 724, 1488
BspCNI	CTCAG	3 cut(s)	432, 459, 1507
BspDI	ATCGAT	1 cut(s)	942
BspFNI	CGCG	3 cut(s)	434, 726, 1255
BspHI	TCATGA	3 cut(s)	274, 1057, 1338
BspLI	GGNNCC	4 cut(s)	364, 374, 646, 1185
BspMAI	CTGCAG	1 cut(s)	490
BspPI	GGATC	1 cut(s)	1283
BspT107I	GGYRCC	2 cut(s)	372, 1183
BspTI	CTTAAG	1 cut(s)	1040
BsrFI	RCCGGY	2 cut(s)	294, 632
BsrI	ACTGG	1 cut(s)	1481
BssAI	RCCGGY	2 cut(s)	294, 632
BssECI	CCNNGG	7 cut(s)	107, 312, 652, 773, 849, 1050, 1360
BssMI	GATC	4 cut(s)	580, 939, 943, 1288
BssNI	GRCGYC	1 cut(s)	373
BssT1I	CCWWGG	1 cut(s)	1050
Bst2UI	CCWGG	2 cut(s)	32, 176
Bst4CI	ACNGT	4 cut(s)	644, 1087, 1438, 1574
Bst6I	CTCTTC	3 cut(s)	1107, 1279, 1396
BstACI	GRCGYC	1 cut(s)	373
BstAFI	CTTAAG	1 cut(s)	1040
BstAPI	GCANNNNNTGC	1 cut(s)	1462
BstBAI	YACGTR	1 cut(s)	549
BstC8I	GCNNGC	8 cut(s)	292, 296, 395, 634, 736, 740, 1013, 1557
BstDEI	CTNAG	4 cut(s)	440, 467, 912, 1515
BstF5I	GGATG	9 cut(s)	24, 204, 454, 514, 561, 583, 1046, 1239, 1380
BstFNI	CGCG	3 cut(s)	434, 726, 1255
BstH2I	RGCGCY	1 cut(s)	376
BstHHI	GCGC	4 cut(s)	375, 436, 690, 1456
BstKTI	GATC	4 cut(s)	583, 942, 946, 1291
BstMAI	GTCTC	5 cut(s)	241, 633, 786, 1109, 1162
BstMBI	GATC	4 cut(s)	580, 939, 943, 1288
BstMCI	CGRYCG	3 cut(s)	637, 790, 942
BstMWI	GCNNNNNNNGC	9 cut(s)	339, 394, 460, 472, 617, 1043, 1426, 1462, 1561
BstNI	CCWGG	2 cut(s)	32, 176
BstNSI	RCATGY	3 cut(s)	48, 142, 1513
BstSCI	CCNGG	7 cut(s)	30, 100, 174, 848, 1176, 1359, 1411
BstSFI	CTRYAG	2 cut(s)	486, 1580
BstSLI	GKGCMC	1 cut(s)	1467
BstSNI	TACGTA	1 cut(s)	549
BstUI	CGCG	3 cut(s)	434, 726, 1255
BstV1I	GCAGC	4 cut(s)	450, 472, 874, 1468
BstV2I	GAAGAC	1 cut(s)	1568
BstX2I	RGATCY	1 cut(s)	1288
BstXI	CCANNNNNNTGG	1 cut(s)	31
BstYI	RGATCY	1 cut(s)	1288
BstZI	CGGCCG	2 cut(s)	634, 787
Bsu15I	ATCGAT	1 cut(s)	942
BsuI	GTATCC	1 cut(s)	1226
BsuTUI	ATCGAT	1 cut(s)	942
BtsCI	GGATG	9 cut(s)	24, 204, 454, 514, 561, 583, 1046, 1239, 1380
BtsI	GCAGTG	3 cut(s)	113, 223, 1465
BtsIMutI	CAGTG	3 cut(s)	113, 223, 1465
BtuMI	TCGCGA	1 cut(s)	1255
Cac8I	GCNNGC	8 cut(s)	292, 296, 395, 634, 736, 740, 1013, 1557
CciI	TCATGA	3 cut(s)	274, 1057, 1338
CfoI	GCGC	4 cut(s)	375, 436, 690, 1456
Cfr10I	RCCGGY	2 cut(s)	294, 632
Cfr13I	GGNCC	6 cut(s)	104, 297, 362, 656, 777, 1099
ClaI	ATCGAT	1 cut(s)	942
Csp6I	GTAC	2 cut(s)	319, 550
CviQI	GTAC	2 cut(s)	319, 550
DdeI	CTNAG	4 cut(s)	440, 467, 912, 1515
DinI	GGCGCC	1 cut(s)	374
DpnI	GATC	4 cut(s)	582, 941, 945, 1290
DpnII	GATC	4 cut(s)	580, 939, 943, 1288
DraIII	CACNNNGTG	1 cut(s)	1180
DriI	GACNNNNNGTC	1 cut(s)	1572
EaeI	YGGCCR	6 cut(s)	177, 634, 713, 721, 787, 846
EagI	CGGCCG	2 cut(s)	634, 787
Eam1104I	CTCTTC	3 cut(s)	1107, 1279, 1396
Eam1105I	GACNNNNNGTC	1 cut(s)	1572
EarI	CTCTTC	3 cut(s)	1107, 1279, 1396
Ecl136II	GAGCTC	3 cut(s)	1037, 1078, 1483
EclXI	CGGCCG	2 cut(s)	634, 787
Eco105I	TACGTA	1 cut(s)	549
Eco130I	CCWWGG	1 cut(s)	1050
Eco24I	GRGCYC	3 cut(s)	1039, 1080, 1485
Eco47I	GGWCC	2 cut(s)	362, 1099
Eco52I	CGGCCG	2 cut(s)	634, 787
Eco53kI	GAGCTC	3 cut(s)	1037, 1078, 1483
Eco57I	CTGAAG	1 cut(s)	383
EcoICRI	GAGCTC	3 cut(s)	1037, 1078, 1483
EcoRII	CCWGG	2 cut(s)	30, 174
EcoT14I	CCWWGG	1 cut(s)	1050
EcoT38I	GRGCYC	3 cut(s)	1039, 1080, 1485
EgeI	GGCGCC	1 cut(s)	374
EheI	GGCGCC	1 cut(s)	374
ErhI	CCWWGG	1 cut(s)	1050
Esp3I	CGTCTC	2 cut(s)	633, 786
FalI	AAGNNNNNCTT	2 cut(s)	1148, 1180
FaqI	GGGAC	3 cut(s)	348, 1181, 1377
FauI	CCCGC	2 cut(s)	637, 1495
FbaI	TGATCA	1 cut(s)	580
FblI	GTMKAC	1 cut(s)	1500
Fnu4HI	GCNGC	7 cut(s)	252, 437, 464, 486, 724, 888, 1457
FokI	GGATG	9 cut(s)	11, 191, 461, 521, 568, 590, 1033, 1226, 1367
FriOI	GRGCYC	3 cut(s)	1039, 1080, 1485
Fsp4HI	GCNGC	7 cut(s)	252, 437, 464, 486, 724, 888, 1457
FspBI	CTAG	4 cut(s)	143, 212, 1193, 1445
GlaI	GCGC	4 cut(s)	374, 435, 689, 1455
GluI	GCNGC	7 cut(s)	252, 437, 464, 486, 724, 888, 1457
GsaI	CCCAGC	1 cut(s)	458
GsuI	CTGGAG	1 cut(s)	1294
HaeII	RGCGCY	1 cut(s)	376
HapII	CCGG	7 cut(s)	102, 295, 633, 849, 1178, 1360, 1412
HhaI	GCGC	4 cut(s)	375, 436, 690, 1456
Hin1I	GRCGYC	1 cut(s)	373
Hin6I	GCGC	4 cut(s)	373, 434, 688, 1454
HinP1I	GCGC	4 cut(s)	373, 434, 688, 1454
HincII	GTYRAC	1 cut(s)	909
HindII	GTYRAC	1 cut(s)	909
HinfI	GANTC	3 cut(s)	4, 1205, 1257
HpaII	CCGG	7 cut(s)	102, 295, 633, 849, 1178, 1360, 1412
Hpy166II	GTNNAC	3 cut(s)	909, 1465, 1501
Hpy188I	TCNGA	8 cut(s)	128, 225, 316, 355, 868, 1034, 1306, 1516
Hpy188III	TCNNGA	8 cut(s)	275, 478, 748, 1058, 1254, 1273, 1328, 1339
Hpy8I	GTNNAC	3 cut(s)	909, 1465, 1501
HpyAV	CCTTC	5 cut(s)	18, 488, 658, 745, 888
HpyCH4III	ACNGT	4 cut(s)	644, 1087, 1438, 1574
HpyCH4IV	ACGT	2 cut(s)	548, 836
HpyCH4V	TGCA	6 cut(s)	393, 488, 611, 890, 1420, 1465
HpyF10VI	GCNNNNNNNGC	9 cut(s)	339, 394, 460, 472, 617, 1043, 1426, 1462, 1561
HpyF3I	CTNAG	4 cut(s)	440, 467, 912, 1515
HpySE526I	ACGT	2 cut(s)	548, 836
Hsp92I	GRCGYC	1 cut(s)	373
HspAI	GCGC	4 cut(s)	373, 434, 688, 1454
KasI	GGCGCC	1 cut(s)	372
KroI	GCCGGC	2 cut(s)	294, 632
KroNI	GCCGGC	2 cut(s)	296, 634
Ksp22I	TGATCA	1 cut(s)	580
Kzo9I	GATC	4 cut(s)	580, 939, 943, 1288
LmnI	GCTCC	4 cut(s)	1083, 1275, 1480, 1488
Lsp1109I	GCAGC	4 cut(s)	450, 472, 874, 1468
LweI	GCATC	1 cut(s)	1055
MaeI	CTAG	4 cut(s)	143, 212, 1193, 1445
MaeII	ACGT	2 cut(s)	548, 836
MaeIII	GTNAC	2 cut(s)	54, 111
MalI	GATC	4 cut(s)	582, 941, 945, 1290
MboI	GATC	4 cut(s)	580, 939, 943, 1288
MboII	GAAGA	7 cut(s)	934, 970, 1073, 1124, 1296, 1413, 1568
MfeI	CAATTG	3 cut(s)	717, 757, 1224
MflI	RGATCY	1 cut(s)	1288
MhlI	GDGCHC	4 cut(s)	1039, 1080, 1467, 1485
MlsI	TGGCCA	2 cut(s)	179, 715
MluCI	AATT	9 cut(s)	283, 717, 757, 963, 1224, 1267, 1319, 1421, 1520
MluNI	TGGCCA	2 cut(s)	179, 715
Mly113I	GGCGCC	1 cut(s)	373
MlyI	GAGTC	1 cut(s)	13
MmeI	TCCRAC	2 cut(s)	193, 248
Mox20I	TGGCCA	2 cut(s)	179, 715
MroNI	GCCGGC	2 cut(s)	294, 632
MscI	TGGCCA	2 cut(s)	179, 715
MseI	TTAA	3 cut(s)	513, 1041, 1473
Msp20I	TGGCCA	2 cut(s)	179, 715
MspA1I	CMGCKG	3 cut(s)	439, 1459, 1490
MspCI	CTTAAG	1 cut(s)	1040
MspI	CCGG	7 cut(s)	102, 295, 633, 849, 1178, 1360, 1412
MspR9I	CCNGG	7 cut(s)	32, 102, 176, 850, 1178, 1361, 1413
MunI	CAATTG	3 cut(s)	717, 757, 1224
MvaI	CCWGG	2 cut(s)	32, 176
MvnI	CGCG	3 cut(s)	434, 726, 1255
MwoI	GCNNNNNNNGC	9 cut(s)	339, 394, 460, 472, 617, 1043, 1426, 1462, 1561
NaeI	GCCGGC	2 cut(s)	296, 634
NarI	GGCGCC	1 cut(s)	373
NciI	CCSGG	5 cut(s)	102, 850, 1178, 1361, 1413
NdeII	GATC	4 cut(s)	580, 939, 943, 1288
NgoMIV	GCCGGC	2 cut(s)	294, 632
NlaIV	GGNNCC	4 cut(s)	364, 374, 646, 1185
NmeAIII	GCCGAG	4 cut(s)	633, 662, 754, 815
NmuCI	GTSAC	2 cut(s)	54, 111
NruI	TCGCGA	1 cut(s)	1255
NspI	RCATGY	3 cut(s)	48, 142, 1513
PagI	TCATGA	3 cut(s)	274, 1057, 1338
PciI	ACATGT	1 cut(s)	1509
PcsI	WCGNNNNNNNCGW	1 cut(s)	792
PdiI	GCCGGC	2 cut(s)	296, 634
PfeI	GAWTC	2 cut(s)	1205, 1257
PflMI	CCANNNNNTGG	2 cut(s)	175, 1087
PkrI	GCNGC	7 cut(s)	253, 438, 465, 487, 725, 889, 1458
Ple19I	CGATCG	1 cut(s)	942
PleI	GAGTC	1 cut(s)	12
PluTI	GGCGCC	1 cut(s)	376
PpsI	GAGTC	1 cut(s)	12
Ppu21I	YACGTR	1 cut(s)	549
PscI	ACATGT	1 cut(s)	1509
Psp124BI	GAGCTC	3 cut(s)	1039, 1080, 1485
Psp1406I	AACGTT	1 cut(s)	836
Psp6I	CCWGG	2 cut(s)	30, 174
PspFI	CCCAGC	1 cut(s)	454
PspGI	CCWGG	2 cut(s)	30, 174
PspN4I	GGNNCC	4 cut(s)	364, 374, 646, 1185
PspPI	GGNCC	6 cut(s)	104, 297, 362, 656, 777, 1099
PstI	CTGCAG	1 cut(s)	490
PsuI	RGATCY	1 cut(s)	1288
PvuI	CGATCG	1 cut(s)	942
PvuII	CAGCTG	1 cut(s)	1459
RruI	TCGCGA	1 cut(s)	1255
RsaI	GTAC	2 cut(s)	320, 551
RsaNI	GTAC	2 cut(s)	319, 550
SacI	GAGCTC	3 cut(s)	1039, 1080, 1485
SaqAI	TTAA	3 cut(s)	513, 1041, 1473
SatI	GCNGC	7 cut(s)	252, 437, 464, 486, 724, 888, 1457
Sau3AI	GATC	4 cut(s)	580, 939, 943, 1288
Sau96I	GGNCC	6 cut(s)	104, 297, 362, 656, 777, 1099
SchI	GAGTC	1 cut(s)	13
ScrFI	CCNGG	7 cut(s)	32, 102, 176, 850, 1178, 1361, 1413
SduI	GDGCHC	4 cut(s)	1039, 1080, 1467, 1485
SfaNI	GCATC	1 cut(s)	1055
SfcI	CTRYAG	2 cut(s)	486, 1580
SfoI	GGCGCC	1 cut(s)	374
SinI	GGWCC	2 cut(s)	362, 1099
SmlI	CTYRAG	4 cut(s)	476, 1040, 1141, 1165
SmoI	CTYRAG	4 cut(s)	476, 1040, 1141, 1165
SnaBI	TACGTA	1 cut(s)	549
SpeI	ACTAGT	1 cut(s)	1192
Sse9I	AATT	9 cut(s)	283, 717, 757, 963, 1224, 1267, 1319, 1421, 1520
SsiI	CCGC	5 cut(s)	252, 437, 630, 724, 1488
SspDI	GGCGCC	1 cut(s)	372
SspMI	CTAG	4 cut(s)	143, 212, 1193, 1445
SstI	GAGCTC	3 cut(s)	1039, 1080, 1485
StyD4I	CCNGG	7 cut(s)	30, 100, 174, 848, 1176, 1359, 1411
StyI	CCWWGG	1 cut(s)	1050
TaaI	ACNGT	4 cut(s)	644, 1087, 1438, 1574
TaiI	ACGT	2 cut(s)	551, 839
TaqI	TCGA	3 cut(s)	160, 942, 1212
TaqII	GACCGA	2 cut(s)	99, 1116
TasI	AATT	9 cut(s)	283, 717, 757, 963, 1224, 1267, 1319, 1421, 1520
TatI	WGTACW	1 cut(s)	318
TauI	GCSGC	3 cut(s)	254, 439, 726
TfiI	GAWTC	2 cut(s)	1205, 1257
Tru1I	TTAA	3 cut(s)	513, 1041, 1473
Tru9I	TTAA	3 cut(s)	513, 1041, 1473
TscAI	CASTG	3 cut(s)	120, 223, 1472
TseFI	GTSAC	2 cut(s)	54, 111
TseI	GCWGC	4 cut(s)	463, 485, 887, 1456
Tsp45I	GTSAC	2 cut(s)	54, 111
TspDTI	ATGAA	6 cut(s)	548, 935, 1074, 1218, 1327, 1355
TspGWI	ACGGA	1 cut(s)	197
TspRI	CASTG	3 cut(s)	120, 223, 1472
Van91I	CCANNNNNTGG	2 cut(s)	175, 1087
Vha464I	CTTAAG	1 cut(s)	1040
VneI	GTGCAC	1 cut(s)	1463
VpaK11BI	GGWCC	2 cut(s)	362, 1099
XapI	RAATTY	3 cut(s)	1267, 1319, 1520
XceI	RCATGY	3 cut(s)	48, 142, 1513
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1088
XmiI	GTMKAC	1 cut(s)	1500
XspI	CTAG	4 cut(s)	143, 212, 1193, 1445

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.