Rh4CG153500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0003968
Physical Location & Seq
Forward (+)
33800306 .. 33801629
1324 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG153500.1

Sequence Viewer

Length: 582 bp
ATGGTGGAAATCAAAGATATGGAAGAGTTTTGGGGAGAATTTCGGATTTCGGGTCAACCCACATATAACAGTCAATGTTTCGCTCTTCAAATACATCATGGAGGTGATTTTGTCAAAGAGGCTGCTGGGCATAAATATGAGGGTGGTCAACTGTGTTGGGCAAAGTTGCTGGACCCAGACAAGATTTGTTGGATAGAGCTCAATACTTTTGCTTGGAGGTTGGGATATAGGAAGCCACTAGTGCACTATTGGTTCAAGCATCCCAACATGTCAGTATTCTTGACGATCACCAAAGATGAGAATGTACAAAAGATGATAAGGCTACTGCCAAGTTCCAGGATTATTCAAATTTATTATGTTGGTAGAGGGCAGAGGCAAGTCGAACTTGTGGAAATGGAGTATATGGATCCCAAACATGAGGAGATTATACCACATGTAGATTTCATTCCTGAGATTGTCTTAAAGCCAATAAAAGCATATGCAAGGAGTGAATGTAGTATAGAAGAAAATGCAATTGGTAAAAGAAAGGCAAGAGACATAGATGAGTGTGAATGTTATAATGAAGATAATGATGACAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DBD_Tnp_Mut MULE SWIM

Protein Analysis

193

Amino Acids

22.76

Weight (kDa)

5.41

Isoelectric Point (pI)

47.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1-like PF26130 27 - 119 1e-14 PB1-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 558
AclWI GGATC 2 cut(s) 401, 414
AcsI RAATTY 2 cut(s) 38, 348
AfaI GTAC 1 cut(s) 306
AflIII ACRYGT 2 cut(s) 267, 433
AgsI TTSAA 3 cut(s) 89, 256, 347
AhlI ACTAGT 1 cut(s) 238
AjnI CCWGG 1 cut(s) 335
AjuI GAANNNNNNNTTGG 2 cut(s) 498, 530
AluBI AGCT 1 cut(s) 199
AluI AGCT 1 cut(s) 199
Alw21I GWGCWC 2 cut(s) 201, 246
Alw26I GTCTC 1 cut(s) 528
Alw44I GTGCAC 1 cut(s) 242
AlwI GGATC 2 cut(s) 401, 414
ApaLI GTGCAC 1 cut(s) 242
ApeKI GCWGC 1 cut(s) 122
ApoI RAATTY 2 cut(s) 38, 348
AspS9I GGNCC 1 cut(s) 172
AsuHPI GGTGA 2 cut(s) 116, 280
AvaII GGWCC 1 cut(s) 172
BaeGI GKGCMC 1 cut(s) 246
BamHI GGATCC 1 cut(s) 406
BanII GRGCYC 1 cut(s) 201
Bbv12I GWGCWC 2 cut(s) 201, 246
BbvI GCAGC 1 cut(s) 109
BciT130I CCWGG 1 cut(s) 337
BcoDI GTCTC 1 cut(s) 528
BcuI ACTAGT 1 cut(s) 238
BfaI CTAG 1 cut(s) 239
BisI GCNGC 1 cut(s) 123
BlsI GCNGC 1 cut(s) 124
Bme1390I CCNGG 1 cut(s) 337
Bme18I GGWCC 1 cut(s) 172
BmgT120I GGNCC 1 cut(s) 172
BmiI GGNNCC 2 cut(s) 174, 408
BmrFI CCNGG 1 cut(s) 337
BmsI GCATC 1 cut(s) 268
BsaXI ACNNNNNCTCC 2 cut(s) 478, 508
BseBI CCWGG 1 cut(s) 337
BseGI GGATG 1 cut(s) 259
BseMII CTCAG 1 cut(s) 441
BseRI GAGGAG 1 cut(s) 434
BseSI GKGCMC 1 cut(s) 246
BseXI GCAGC 1 cut(s) 109
BseYI CCCAGC 1 cut(s) 125
BsiHKAI GWGCWC 2 cut(s) 201, 246
BsmAI GTCTC 1 cut(s) 528
Bsp1286I GDGCHC 2 cut(s) 201, 246
Bsp1407I TGTACA 1 cut(s) 304
Bsp143I GATC 2 cut(s) 285, 406
BspCNI CTCAG 1 cut(s) 442
BspLI GGNNCC 2 cut(s) 174, 408
BspPI GGATC 2 cut(s) 401, 414
BspQI GCTCTTC 1 cut(s) 90
BsrGI TGTACA 1 cut(s) 304
BssMI GATC 2 cut(s) 285, 406
Bst2UI CCWGG 1 cut(s) 337
Bst4CI ACNGT 2 cut(s) 71, 153
Bst6I CTCTTC 2 cut(s) 18, 90
BstAUI TGTACA 1 cut(s) 304
BstDEI CTNAG 1 cut(s) 450
BstF5I GGATG 1 cut(s) 259
BstKTI GATC 2 cut(s) 288, 409
BstMAI GTCTC 1 cut(s) 528
BstMBI GATC 2 cut(s) 285, 406
BstMWI GCNNNNNNNGC 1 cut(s) 241
BstNI CCWGG 1 cut(s) 337
BstNSI RCATGY 2 cut(s) 271, 437
BstSCI CCNGG 1 cut(s) 335
BstSLI GKGCMC 1 cut(s) 246
BstV1I GCAGC 1 cut(s) 109
BstX2I RGATCY 1 cut(s) 406
BstYI RGATCY 1 cut(s) 406
BtsCI GGATG 1 cut(s) 259
Cfr13I GGNCC 1 cut(s) 172
Csp6I GTAC 1 cut(s) 305
CviAII CATG 4 cut(s) 98, 268, 416, 434
CviJI RGCY 5 cut(s) 122, 199, 235, 322, 466
CviKI_1 RGCY 5 cut(s) 122, 199, 235, 322, 466
CviQI GTAC 1 cut(s) 305
DdeI CTNAG 1 cut(s) 450
DpnI GATC 2 cut(s) 287, 408
DpnII GATC 2 cut(s) 285, 406
Eam1104I CTCTTC 2 cut(s) 18, 90
EarI CTCTTC 2 cut(s) 18, 90
Ecl136II GAGCTC 1 cut(s) 199
Eco24I GRGCYC 1 cut(s) 201
Eco47I GGWCC 1 cut(s) 172
Eco53kI GAGCTC 1 cut(s) 199
EcoICRI GAGCTC 1 cut(s) 199
EcoRII CCWGG 1 cut(s) 335
EcoT38I GRGCYC 1 cut(s) 201
FaeI CATG 4 cut(s) 101, 271, 419, 437
FalI AAGNNNNNCTT 2 cut(s) 369, 401
FatI CATG 4 cut(s) 97, 267, 415, 433
FauNDI CATATG 1 cut(s) 478
Fnu4HI GCNGC 1 cut(s) 123
FokI GGATG 1 cut(s) 246
FriOI GRGCYC 1 cut(s) 201
Fsp4HI GCNGC 1 cut(s) 123
FspBI CTAG 1 cut(s) 239
GluI GCNGC 1 cut(s) 123
GsaI CCCAGC 1 cut(s) 129
Hin1II CATG 4 cut(s) 101, 271, 419, 437
HincII GTYRAC 2 cut(s) 56, 149
HindII GTYRAC 2 cut(s) 56, 149
HphI GGTGA 2 cut(s) 116, 280
Hpy166II GTNNAC 3 cut(s) 56, 149, 244
Hpy188I TCNGA 1 cut(s) 45
Hpy188III TCNNGA 2 cut(s) 280, 449
Hpy8I GTNNAC 3 cut(s) 56, 149, 244
HpyCH4III ACNGT 2 cut(s) 71, 153
HpyCH4V TGCA 3 cut(s) 244, 482, 512
HpyF10VI GCNNNNNNNGC 1 cut(s) 241
HpyF3I CTNAG 1 cut(s) 450
Hsp92II CATG 4 cut(s) 101, 271, 419, 437
Kzo9I GATC 2 cut(s) 285, 406
LguI GCTCTTC 1 cut(s) 90
LpnPI CCDG 6 cut(s) 111, 155, 189, 322, 349, 462
Lsp1109I GCAGC 1 cut(s) 109
LweI GCATC 1 cut(s) 268
MaeI CTAG 1 cut(s) 239
MalI GATC 2 cut(s) 287, 408
MboI GATC 2 cut(s) 285, 406
MboII GAAGA 4 cut(s) 35, 77, 515, 575
MfeI CAATTG 1 cut(s) 513
MflI RGATCY 1 cut(s) 406
MhlI GDGCHC 2 cut(s) 201, 246
MluCI AATT 4 cut(s) 38, 348, 513, 577
MmeI TCCRAC 1 cut(s) 170
MnlI CCTC 7 cut(s) 95, 112, 133, 210, 359, 366, 412
MseI TTAA 2 cut(s) 461, 580
MslI CAYNNNNRTG 2 cut(s) 102, 135
MspR9I CCNGG 1 cut(s) 337
MunI CAATTG 1 cut(s) 513
MvaI CCWGG 1 cut(s) 337
MwoI GCNNNNNNNGC 1 cut(s) 241
NdeI CATATG 1 cut(s) 478
NdeII GATC 2 cut(s) 285, 406
NlaIII CATG 4 cut(s) 101, 271, 419, 437
NlaIV GGNNCC 2 cut(s) 174, 408
NspI RCATGY 2 cut(s) 271, 437
PciI ACATGT 2 cut(s) 267, 433
PciSI GCTCTTC 1 cut(s) 90
PfoI TCCNGGA 1 cut(s) 335
PkrI GCNGC 1 cut(s) 124
PscI ACATGT 2 cut(s) 267, 433
PsiI TTATAA 1 cut(s) 558
Psp124BI GAGCTC 1 cut(s) 201
Psp6I CCWGG 1 cut(s) 335
PspFI CCCAGC 1 cut(s) 125
PspGI CCWGG 1 cut(s) 335
PspN4I GGNNCC 2 cut(s) 174, 408
PspPI GGNCC 1 cut(s) 172
PsuI RGATCY 1 cut(s) 406
RsaI GTAC 1 cut(s) 306
RsaNI GTAC 1 cut(s) 305
RseI CAYNNNNRTG 2 cut(s) 102, 135
SacI GAGCTC 1 cut(s) 201
SapI GCTCTTC 1 cut(s) 90
SaqAI TTAA 2 cut(s) 461, 580
SatI GCNGC 1 cut(s) 123
Sau3AI GATC 2 cut(s) 285, 406
Sau96I GGNCC 1 cut(s) 172
ScrFI CCNGG 1 cut(s) 337
SduI GDGCHC 2 cut(s) 201, 246
SetI ASST 3 cut(s) 106, 201, 221
SfaNI GCATC 1 cut(s) 268
SinI GGWCC 1 cut(s) 172
SmiMI CAYNNNNRTG 2 cut(s) 102, 135
SpeI ACTAGT 1 cut(s) 238
Sse9I AATT 4 cut(s) 38, 348, 513, 577
SspMI CTAG 1 cut(s) 239
SstI GAGCTC 1 cut(s) 201
StyD4I CCNGG 1 cut(s) 335
TaaI ACNGT 2 cut(s) 71, 153
TaqI TCGA 1 cut(s) 381
TasI AATT 4 cut(s) 38, 348, 513, 577
TatI WGTACW 1 cut(s) 304
Tru1I TTAA 2 cut(s) 461, 580
Tru9I TTAA 2 cut(s) 461, 580
TseI GCWGC 1 cut(s) 122
TspDTI ATGAA 2 cut(s) 433, 576
VneI GTGCAC 1 cut(s) 242
VpaK11BI GGWCC 1 cut(s) 172
XapI RAATTY 2 cut(s) 38, 348
XceI RCATGY 2 cut(s) 271, 437
XspI CTAG 1 cut(s) 239
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.